Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0014328

Physical Location & Seq

Genomic Coordinates Forward (+)

41723865 .. 41750627

Gene Structure 26763 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1283100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2775 bp

ATGATACAGTCAGTCTTCTTCCCCAACTCTCTCTCCTCTATCTCTTTTCCACGCACAGAGAGAAAGCACAGCGGAGGCAGTGAGTGGCAAATGGTTGCCCAAAGAGGCAGCCCATGGGGGCTCCGATGTTTCTTCCTCTGCCTGGCAGTTCAATTTGCTCTGGAGGCGGCCGCCGAGTACTTCAAGCCATTCAACGTCAGCTACGACCACCGAGCTCTGATCATCGACGGCAAGCGCCGCATGCTCGTCTCCGCCGGAATCCACTACCCCCGCGCCACTCCTGAGATGTGGCCTGATCTGATCGCAAAAAGCAAGGAAGGCGGGGTGGATGTGATCCAAACTTATGCATTTTGGAGCGGCCATGAGCCAGTGAGAGGACAGTACAACTTTGAAGGGAGATATGATATTGTCAAGTTTGCAAATTTAGTGGGAGCCAGTGGACTCTATCTCCATCTACGCATAGGGCCCTATGTCTGCGCTGAGTGGAACTTTGGTGGCTTCCCCGTGTGGTTGCGTGACATCCCTGGAATAGAGTTTCGAACAAACAATGCGCTTTTTAAGGAGGAGATGCAGCGGTTTGTTAAAAAGATGGTGGATCTGATGCAAGAGGAAGAACTATTATCTTGGCAAGGTGGTCCAATTATCATGCTGCAGATTGAAAATGAATATGGAAATATTGAAGCGCAATTTGGTCAGAAAGGGAAAGAATATATTAAGTGGGCTGCTGAGATGGCTTTAGGTCTCGGTGCTGGGGTTCCATGGGTCATGTGCAAGCAAGTTGATGCTCCAGGAAGTATTATAGATGCATGCAACGGCTACTATTGTGATGGTTACAAGCCAAATTCCTATAACAAACCAACAATGTGGACAGAAGATTGGGATGGATGGTATGCATCATGGGGTGGAAGATTGCCTCACAGGCCTGTTGAAGATCTTGCATTTGCAGTAGCACGCTTTTACCAACGTGGAGGGAGCTTTCAGAATTATTATATGTATTTTGGTGGAACAAACTTTGGGCGGACTTCTGGGGGCCCGTTTTATATTACTAGTTATGATTATGATGCTCCAATTGATGAATATGGTCTGCTGAGTGAGCCAAAATGGGGACATTTGAAGGATCTACATGCTGCTATAAAGCTCTGCGAACCTGCTCTTGTTGCTGCTGATTCTCCGAATTATATAAAGTTGGGACCAAAACAAGAGGCACACGTGTACCGCATGAATTCTCACACCAAAGGCCTGAACATTACTTCGTATGGAAGTCAAATCAGTTGCTCCGCATTCCTTGCAAACATTGATGAACATAAAGCAGCTTCTGTAACATTCCTTGGTCAAAAGTATAATCTACCACCATGGTCAGTCAGTATTTTGCCAGACTGCCGGAACGTGGTGTACAACACTGCCAAGGTTGGTGCACAAACTTCCATCAAAACAGTAGAGTTTGATTTGCCCCTTTATTCAGGTATTTCTTCACAGCAACAATTCATTACCAAGAATGATGATTTGTTTATCACCGAATCATGGATGACTGTAAACGAGCCAGTTGGTGTTTGGAGTGAAAACAATTTTACGGTCCAAGGAATATTGGAGCATTTGAATGTAACAAAAGACCAGTCTGATTACTTATGGCATATAACCAGGATTTTTGTTTCAGAGGATGACATATCTTTTTGGGAGAAAAACAATATTAGCCCAGCAGTCTCAATTGATAGCATGCGCGACGTGTTGCGTGTATTTGTTAATGGCCAGCTTACAGGCAGTGTCATTGGTCACTGGGTCAAGGTGGAGCAGCCTGTTAAGTTTCTGAAGGGATACAATGATTTGGTGTTGTTAACTCAGACAGTGGGCTTGCAGAACTATGGTGCTTTCCTGGAGAAAGATGGGGCAGGATTTAGAGGACAGATTAAGCTTACTGGCTTTAAAAATGGTGATATTAACTTGTCAAATTTGCTATGGACTTACCAGGTTGGGCTTAAGGGCGAGTTTTTGAAAATATACACCATAGAAGAAAATGAAAAGGCAAGTTGGGCTGAGATATCGCCAGATGATGATCCATCCACGTTTATATGGTACAAGACATACTTTGATAGCCCTGCTGGCACAGATCCAGTTGCTCTTGATTTAGGAAGCATGGGTAAGGGCCAGGCTTGGGTCAATGGCCACCATATAGGACGATATTGGACACTTGTTGCTCCAGAAGATGGATGTCCAGAAATCTGTGATTACCGTGGGGCATACAACTCCGATAAGTGCTCATTTAATTGTGGGAAGCCTACTCAAACCTTGTACCATGTACCGAGATCATGGTTACAGTCTTCAAGTAATTTACTTGTTATTTTGGAGGAAACTGGCGGGAATCCATTTGATATTTCAATCAAGTTGCGATCAGCCAGAGTCCTCTGTGCTCAAGTGTCAGAGTCTCACTACCCGCCTGTTCAGAAGTGGTTCAATCCAGACTCTGCGGATGAAAAAATTACAGTAAACGATCTGACACCAGAAATGCACTTGCAATGTCAAGATGGGTTTACGATATCTTCCATAGAATTTGCCAGCTATGGAACACCTCAAGGTAGCTGCCAGAAGTTTTCTATGGGAAATTGCCATGCAACCAATTCATTGTCCATTGTCTCCAAGAGTTGCCTGGGAAAGAATAGCTGTTCAGTTGAAATATCAAATATCAGTTTTGGAGGTGACCCATGCCGAGGCGTTGTTAAGACGTTGGCAGTTGAGGCAAGATGCAGGTCATCATCATTAGTTGGTTTGTCTCAGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0009505 GO:0015925 GO:0016020 GO:0016787 GO:0016798 GO:0030312 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

925

Amino Acids

103.57

Weight (kDa)

5.69

Isoelectric Point (pI)

40.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	73 - 377	2e-115	Glycosyl hydrolases family 35
GHD	PF17834	419 - 470	7.5e-24	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	689 - 775	1.1e-24	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	836 - 913	6.2e-23	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014328)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32810 AT2G32810
fragaria_vesca	FvH4_4g13490
malus_domestica	MD13G1283100.v1.1
prunus_persica	Prupe.1G160600_v2.0.a1 Prupe.1G160600_v2.0.a1
pyrus_communis	pycom420g01190
rosa_chinensis	RchiOBHm_Chr4g0414111
rosa_laevigata	RLG00000008175
rosa_multiflora	Rmu_sc0003264.1_g000008
rosa_roxburghii	Rroxscaffold_5G00358040
rosa_rugosa	Rorug04G0125600 Rorug04G0125700
rosa_samantha	Rh4AG184200 Rh4BG183700 Rh4DG179600
rosa_wichuraiana	Rw4G015680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1156, 2730
AccB7I	CCANNNNNTGG	1 cut(s)	2201
AccBSI	CCGCTC	1 cut(s)	357
AccII	CGCG	2 cut(s)	273, 1719
AclWI	GGATC	5 cut(s)	328, 603, 1125, 2045, 2099
AcoI	YGGCCR	4 cut(s)	168, 358, 1744, 2158
AcsI	RAATTY	5 cut(s)	421, 841, 1222, 1945, 2543
AcuI	CTGAAG	1 cut(s)	1826
AcvI	CACGTG	1 cut(s)	1210
AfaI	GTAC	7 cut(s)	179, 383, 1214, 1394, 2072, 2288, 2295
AfiI	CCNNNNNNNGG	8 cut(s)	142, 374, 1103, 1387, 1521, 2149, 2201, 2702
AflII	CTTAAG	1 cut(s)	1973
AflIII	ACRYGT	3 cut(s)	1207, 1209, 1722
AhlI	ACTAGT	1 cut(s)	1046
AjiI	CACGTC	1 cut(s)	1723
AjnI	CCWGG	8 cut(s)	141, 523, 787, 1637, 1869, 1962, 2142, 2640
AjuI	GAANNNNNNNTTGG	2 cut(s)	672, 704
Alw21I	GWGCWC	4 cut(s)	217, 1417, 2255, 2407
Alw26I	GTCTC	6 cut(s)	253, 746, 1705, 2424, 2632, 2769
Alw44I	GTGCAC	1 cut(s)	1413
AlwI	GGATC	5 cut(s)	328, 603, 1125, 2045, 2099
AlwNI	CAGNNNCTG	2 cut(s)	1316, 2459
ApaI	GGGCCC	2 cut(s)	468, 1034
ApaLI	GTGCAC	1 cut(s)	1413
ApeKI	GCWGC	9 cut(s)	108, 571, 649, 722, 1127, 1160, 1310, 1789, 2574
ApoI	RAATTY	5 cut(s)	421, 841, 1222, 1945, 2543
ArsI	GACNNNNNNTTYG	2 cut(s)	1598, 1630
Asp700I	GAANNNNTTC	1 cut(s)	2444
AspLEI	GCGC	6 cut(s)	237, 275, 479, 553, 685, 1719
AspS9I	GGNCC	8 cut(s)	464, 465, 635, 1030, 1031, 1190, 1573, 2140
AsuHPI	GGTGA	3 cut(s)	1504, 1940, 2702
AsuII	TTCGAA	1 cut(s)	538
AvaII	GGWCC	3 cut(s)	635, 1190, 1573
BaeGI	GKGCMC	3 cut(s)	468, 1034, 1417
BaeI	ACNNNNGTAYC	2 cut(s)	1196, 1229
BalI	TGGCCA	2 cut(s)	1746, 2160
BanII	GRGCYC	4 cut(s)	123, 217, 468, 1034
BbrPI	CACGTG	1 cut(s)	1210
BbsI	GAAGAC	2 cut(s)	7, 2307
Bbv12I	GWGCWC	4 cut(s)	217, 1417, 2255, 2407
BbvI	GCAGC	9 cut(s)	120, 583, 636, 709, 1114, 1147, 1322, 1801, 2561
BceAI	ACGGC	2 cut(s)	244, 829
BciT130I	CCWGG	8 cut(s)	143, 525, 789, 1639, 1871, 1964, 2144, 2642
BciVI	GTATCC	1 cut(s)	1805
BclI	TGATCA	1 cut(s)	219
BcoDI	GTCTC	6 cut(s)	253, 746, 1705, 2424, 2632, 2769
BcuI	ACTAGT	1 cut(s)	1046
BfaI	CTAG	1 cut(s)	1047
BfmI	CTRYAG	1 cut(s)	650
BfoI	RGCGCY	1 cut(s)	238
BfrI	CTTAAG	1 cut(s)	1973
BfuAI	ACCTGC	2 cut(s)	1156, 2730
BfuI	GTATCC	1 cut(s)	1805
BglI	GCCNNNNNGGC	2 cut(s)	919, 2097
BglII	AGATCT	1 cut(s)	931
BmcAI	AGTACT	1 cut(s)	179
Bme1390I	CCNGG	8 cut(s)	143, 525, 789, 1639, 1871, 1964, 2144, 2642
Bme18I	GGWCC	3 cut(s)	635, 1190, 1573
BmgBI	CACGTC	1 cut(s)	1723
BmgT120I	GGNCC	8 cut(s)	464, 465, 635, 1030, 1031, 1190, 1573, 2140
BmiI	GGNNCC	7 cut(s)	122, 433, 466, 756, 1031, 1032, 1191
BmrFI	CCNGG	8 cut(s)	143, 525, 789, 1639, 1871, 1964, 2144, 2642
BmrI	ACTGGG	1 cut(s)	1783
BmsI	GCATC	7 cut(s)	558, 591, 772, 793, 902, 1051, 2726
BmuI	ACTGGG	1 cut(s)	1783
BpiI	GAAGAC	2 cut(s)	7, 2307
BpmI	CTGGAG	4 cut(s)	182, 771, 1892, 2178
Bpu14I	TTCGAA	1 cut(s)	538
BpuEI	CTTGAG	2 cut(s)	2391, 2550
BsaAI	YACGTR	1 cut(s)	1210
BsaBI	GATNNNNATC	1 cut(s)	2049
BsaI	GGTCTC	1 cut(s)	746
BsaXI	ACNNNNNCTCC	4 cut(s)	17, 47, 432, 462
Bsc4I	CCNNNNNNNGG	8 cut(s)	142, 374, 1103, 1387, 1521, 2149, 2201, 2702
Bse1I	ACTGG	8 cut(s)	368, 435, 1541, 1612, 1778, 1918, 2108, 2353
Bse3DI	GCAATG	1 cut(s)	2516
Bse8I	GATNNNNATC	1 cut(s)	2049
BseBI	CCWGG	8 cut(s)	143, 525, 789, 1639, 1871, 1964, 2144, 2642
BseGI	GGATG	9 cut(s)	334, 519, 886, 890, 1530, 1663, 2054, 2210, 2470
BseJI	GATNNNNATC	1 cut(s)	2049
BseLI	CCNNNNNNNGG	8 cut(s)	142, 374, 1103, 1387, 1521, 2149, 2201, 2702
BseMI	GCAATG	1 cut(s)	2516
BseMII	CTCAG	6 cut(s)	273, 471, 717, 1079, 1850, 2022
BseNI	ACTGG	8 cut(s)	368, 435, 1541, 1612, 1778, 1918, 2108, 2353
BseRI	GAGGAG	2 cut(s)	25, 578
BseSI	GKGCMC	3 cut(s)	468, 1034, 1417
BseX3I	CGGCCG	1 cut(s)	168
BseXI	GCAGC	9 cut(s)	120, 583, 636, 709, 1114, 1147, 1322, 1801, 2561
BseYI	CCCAGC	2 cut(s)	749, 1693
Bsh1236I	CGCG	2 cut(s)	273, 1719
Bsh1285I	CGRYCG	1 cut(s)	171
BsiEI	CGRYCG	1 cut(s)	171
BsiHKAI	GWGCWC	4 cut(s)	217, 1417, 2255, 2407
BsiSI	CCGG	2 cut(s)	255, 1381
BslFI	GGGAC	2 cut(s)	1119, 1203
BslI	CCNNNNNNNGG	8 cut(s)	142, 374, 1103, 1387, 1521, 2149, 2201, 2702
BsmAI	GTCTC	6 cut(s)	253, 746, 1705, 2424, 2632, 2769
BsmBI	CGTCTC	1 cut(s)	253
BsmFI	GGGAC	2 cut(s)	1119, 1203
BsmI	GAATGC	1 cut(s)	1280
Bso31I	GGTCTC	1 cut(s)	746
Bsp119I	TTCGAA	1 cut(s)	538
Bsp120I	GGGCCC	2 cut(s)	464, 1030
Bsp1286I	GDGCHC	7 cut(s)	123, 217, 468, 1034, 1417, 2255, 2407
Bsp1407I	TGTACA	1 cut(s)	1392
Bsp19I	CCATGG	3 cut(s)	113, 758, 1352
BspCNI	CTCAG	6 cut(s)	274, 472, 718, 1080, 1849, 2023
BspFNI	CGCG	2 cut(s)	273, 1719
BspLI	GGNNCC	7 cut(s)	122, 433, 466, 756, 1031, 1032, 1191
BspMAI	CTGCAG	1 cut(s)	654
BspMI	ACCTGC	2 cut(s)	1156, 2730
BspPI	GGATC	5 cut(s)	328, 603, 1125, 2045, 2099
BspT104I	TTCGAA	1 cut(s)	538
BspTI	CTTAAG	1 cut(s)	1973
BspTNI	GGTCTC	1 cut(s)	746
BsrBI	CCGCTC	1 cut(s)	357
BsrDI	GCAATG	1 cut(s)	2516
BsrGI	TGTACA	1 cut(s)	1392
BsrI	ACTGG	8 cut(s)	368, 435, 1541, 1612, 1778, 1918, 2108, 2353
BssT1I	CCWWGG	6 cut(s)	113, 758, 1327, 1352, 1404, 1576
Bst2UI	CCWGG	8 cut(s)	143, 525, 789, 1639, 1871, 1964, 2144, 2642
Bst4CI	ACNGT	9 cut(s)	9, 381, 1435, 1531, 1573, 1843, 2228, 2313, 2479
BstAFI	CTTAAG	1 cut(s)	1973
BstAPI	GCANNNNNTGC	1 cut(s)	1286
BstAUI	TGTACA	1 cut(s)	1392
BstBAI	YACGTR	1 cut(s)	1210
BstBI	TTCGAA	1 cut(s)	538
BstDEI	CTNAG	7 cut(s)	282, 480, 726, 1088, 1836, 2031, 2766
BstDSI	CCRYGG	4 cut(s)	113, 758, 1352, 2227
BstEII	GGTNACC	1 cut(s)	2690
BstF5I	GGATG	9 cut(s)	334, 519, 886, 890, 1530, 1663, 2054, 2210, 2470
BstFNI	CGCG	2 cut(s)	273, 1719
BstH2I	RGCGCY	1 cut(s)	238
BstHHI	GCGC	6 cut(s)	237, 275, 479, 553, 685, 1719
BstMAI	GTCTC	6 cut(s)	253, 746, 1705, 2424, 2632, 2769
BstMCI	CGRYCG	1 cut(s)	171
BstNI	CCWGG	8 cut(s)	143, 525, 789, 1639, 1871, 1964, 2144, 2642
BstNSI	RCATGY	4 cut(s)	244, 810, 1127, 1717
BstPI	GGTNACC	1 cut(s)	2690
BstSCI	CCNGG	8 cut(s)	141, 523, 787, 1637, 1869, 1962, 2142, 2640
BstSFI	CTRYAG	1 cut(s)	650
BstSLI	GKGCMC	3 cut(s)	468, 1034, 1417
BstUI	CGCG	2 cut(s)	273, 1719
BstV1I	GCAGC	9 cut(s)	120, 583, 636, 709, 1114, 1147, 1322, 1801, 2561
BstV2I	GAAGAC	2 cut(s)	7, 2307
BstX2I	RGATCY	4 cut(s)	595, 931, 1117, 2104
BstXI	CCANNNNNNTGG	1 cut(s)	864
BstYI	RGATCY	4 cut(s)	595, 931, 1117, 2104
BstZI	CGGCCG	1 cut(s)	168
BsuI	GTATCC	1 cut(s)	1805
BtgI	CCRYGG	4 cut(s)	113, 758, 1352, 2227
BtrI	CACGTC	1 cut(s)	1723
BtsCI	GGATG	9 cut(s)	334, 519, 886, 890, 1530, 1663, 2054, 2210, 2470
BtsI	GCAGTG	3 cut(s)	85, 1398, 1765
BtsIMutI	CAGTG	7 cut(s)	85, 375, 442, 1398, 1765, 1771, 1848
BveI	ACCTGC	2 cut(s)	1156, 2730
CaiI	CAGNNNCTG	2 cut(s)	1316, 2459
CciNI	GCGGCCGC	1 cut(s)	168
CfoI	GCGC	6 cut(s)	237, 275, 479, 553, 685, 1719
Cfr13I	GGNCC	8 cut(s)	464, 465, 635, 1030, 1031, 1190, 1573, 2140
CsiI	ACCWGGT	1 cut(s)	1962
Csp6I	GTAC	7 cut(s)	178, 382, 1213, 1393, 2071, 2287, 2294
CspCI	CAANNNNNGTGG	2 cut(s)	408, 443
CviQI	GTAC	7 cut(s)	178, 382, 1213, 1393, 2071, 2287, 2294
DdeI	CTNAG	7 cut(s)	282, 480, 726, 1088, 1836, 2031, 2766
DraI	TTTAAA	1 cut(s)	1921
EaeI	YGGCCR	4 cut(s)	168, 358, 1744, 2158
EagI	CGGCCG	1 cut(s)	168
EciI	GGCGGA	2 cut(s)	241, 1033
Ecl136II	GAGCTC	1 cut(s)	215
EclXI	CGGCCG	1 cut(s)	168
Eco130I	CCWWGG	6 cut(s)	113, 758, 1327, 1352, 1404, 1576
Eco147I	AGGCCT	2 cut(s)	922, 1239
Eco24I	GRGCYC	4 cut(s)	123, 217, 468, 1034
Eco31I	GGTCTC	1 cut(s)	746
Eco32I	GATATC	2 cut(s)	2037, 2532
Eco47I	GGWCC	3 cut(s)	635, 1190, 1573
Eco52I	CGGCCG	1 cut(s)	168
Eco53kI	GAGCTC	1 cut(s)	215
Eco57I	CTGAAG	1 cut(s)	1826
Eco72I	CACGTG	1 cut(s)	1210
Eco91I	GGTNACC	1 cut(s)	2690
EcoICRI	GAGCTC	1 cut(s)	215
EcoO109I	RGGNCCY	3 cut(s)	464, 465, 1030
EcoO65I	GGTNACC	1 cut(s)	2690
EcoRI	GAATTC	1 cut(s)	1222
EcoRII	CCWGG	8 cut(s)	141, 523, 787, 1637, 1869, 1962, 2142, 2640
EcoRV	GATATC	2 cut(s)	2037, 2532
EcoT14I	CCWWGG	6 cut(s)	113, 758, 1327, 1352, 1404, 1576
EcoT22I	ATGCAT	3 cut(s)	349, 808, 895
EcoT38I	GRGCYC	4 cut(s)	123, 217, 468, 1034
ErhI	CCWWGG	6 cut(s)	113, 758, 1327, 1352, 1404, 1576
Esp3I	CGTCTC	1 cut(s)	253
FalI	AAGNNNNNCTT	2 cut(s)	2066, 2098
FaqI	GGGAC	2 cut(s)	1119, 1203
FauI	CCCGC	4 cut(s)	278, 314, 2345, 2436
FbaI	TGATCA	1 cut(s)	219
FokI	GGATG	9 cut(s)	341, 506, 893, 897, 1537, 1670, 2041, 2217, 2477
FriOI	GRGCYC	4 cut(s)	123, 217, 468, 1034
FspBI	CTAG	1 cut(s)	1047
GlaI	GCGC	6 cut(s)	236, 274, 478, 552, 684, 1718
GsaI	CCCAGC	2 cut(s)	753, 1697
GsuI	CTGGAG	4 cut(s)	182, 771, 1892, 2178
HaeII	RGCGCY	1 cut(s)	238
HapII	CCGG	2 cut(s)	255, 1381
HhaI	GCGC	6 cut(s)	237, 275, 479, 553, 685, 1719
Hin6I	GCGC	6 cut(s)	235, 273, 477, 551, 683, 1717
HinP1I	GCGC	6 cut(s)	235, 273, 477, 551, 683, 1717
HincII	GTYRAC	1 cut(s)	1833
HindII	GTYRAC	1 cut(s)	1833
HindIII	AAGCTT	1 cut(s)	1907
HinfI	GANTC	8 cut(s)	258, 441, 1166, 1517, 2356, 2394, 2417, 2456
HpaI	GTTAAC	1 cut(s)	1833
HpaII	CCGG	2 cut(s)	255, 1381
HphI	GGTGA	3 cut(s)	1504, 1940, 2702
Hpy166II	GTNNAC	9 cut(s)	440, 867, 1213, 1393, 1415, 1534, 1833, 2482, 2526
Hpy188III	TCNNGA	7 cut(s)	161, 281, 2117, 2195, 2210, 2453, 2516
Hpy8I	GTNNAC	9 cut(s)	440, 867, 1213, 1393, 1415, 1534, 1833, 2482, 2526
Hpy99I	CGWCG	2 cut(s)	230, 1724
HpyAV	CCTTC	4 cut(s)	311, 386, 1108, 1801
HpyCH4III	ACNGT	9 cut(s)	9, 381, 1435, 1531, 1573, 1843, 2228, 2313, 2479
HpyCH4IV	ACGT	7 cut(s)	195, 964, 1209, 1386, 1722, 2060, 2717
HpyF3I	CTNAG	7 cut(s)	282, 480, 726, 1088, 1836, 2031, 2766
HpySE526I	ACGT	7 cut(s)	195, 964, 1209, 1386, 1722, 2060, 2717
HspAI	GCGC	6 cut(s)	235, 273, 477, 551, 683, 1717
Ksp22I	TGATCA	1 cut(s)	219
KspAI	GTTAAC	1 cut(s)	1833
Lsp1109I	GCAGC	9 cut(s)	120, 583, 636, 709, 1114, 1147, 1322, 1801, 2561
LweI	GCATC	7 cut(s)	558, 591, 772, 793, 902, 1051, 2726
MabI	ACCWGGT	1 cut(s)	1962
MaeI	CTAG	1 cut(s)	1047
MaeII	ACGT	7 cut(s)	195, 964, 1209, 1386, 1722, 2060, 2717
MaeIII	GTNAC	7 cut(s)	515, 830, 1318, 1600, 1769, 2307, 2690
MbiI	CCGCTC	1 cut(s)	357
MfeI	CAATTG	2 cut(s)	1068, 1704
MflI	RGATCY	4 cut(s)	595, 931, 1117, 2104
MhlI	GDGCHC	7 cut(s)	123, 217, 468, 1034, 1417, 2255, 2407
MlsI	TGGCCA	2 cut(s)	1746, 2160
MluNI	TGGCCA	2 cut(s)	1746, 2160
MlyI	GAGTC	4 cut(s)	435, 2403, 2426, 2450
Mox20I	TGGCCA	2 cut(s)	1746, 2160
Mph1103I	ATGCAT	3 cut(s)	349, 808, 895
MroXI	GAANNNNTTC	1 cut(s)	2444
MscI	TGGCCA	2 cut(s)	1746, 2160
MslI	CAYNNNNRTG	1 cut(s)	1596
Msp20I	TGGCCA	2 cut(s)	1746, 2160
MspA1I	CMGCKG	2 cut(s)	72, 574
MspCI	CTTAAG	1 cut(s)	1973
MspI	CCGG	2 cut(s)	255, 1381
MspR9I	CCNGG	8 cut(s)	143, 525, 789, 1639, 1871, 1964, 2144, 2642
MunI	CAATTG	2 cut(s)	1068, 1704
Mva1269I	GAATGC	1 cut(s)	1280
MvaI	CCWGG	8 cut(s)	143, 525, 789, 1639, 1871, 1964, 2144, 2642
MvnI	CGCG	2 cut(s)	273, 1719
NcoI	CCATGG	3 cut(s)	113, 758, 1352
NlaIV	GGNNCC	7 cut(s)	122, 433, 466, 756, 1031, 1032, 1191
NmeAIII	GCCGAG	2 cut(s)	199, 2726
NmuCI	GTSAC	3 cut(s)	515, 1769, 2690
NotI	GCGGCCGC	1 cut(s)	168
NsiI	ATGCAT	3 cut(s)	349, 808, 895
NspI	RCATGY	4 cut(s)	244, 810, 1127, 1717
NspV	TTCGAA	1 cut(s)	538
PaeI	GCATGC	3 cut(s)	244, 810, 1717
PceI	AGGCCT	2 cut(s)	922, 1239
PcsI	WCGNNNNNNNCGW	1 cut(s)	201
PctI	GAATGC	1 cut(s)	1280
PdmI	GAANNNNTTC	1 cut(s)	2444
PfeI	GAWTC	4 cut(s)	258, 1166, 1517, 2356
PflMI	CCANNNNNTGG	1 cut(s)	2201
PfoI	TCCNGGA	2 cut(s)	787, 1869
PleI	GAGTC	4 cut(s)	435, 2402, 2425, 2450
PmaCI	CACGTG	1 cut(s)	1210
PmlI	CACGTG	1 cut(s)	1210
PpsI	GAGTC	4 cut(s)	435, 2402, 2425, 2450
Ppu21I	YACGTR	1 cut(s)	1210
Psp124BI	GAGCTC	1 cut(s)	217
Psp6I	CCWGG	8 cut(s)	141, 523, 787, 1637, 1869, 1962, 2142, 2640
PspCI	CACGTG	1 cut(s)	1210
PspEI	GGTNACC	1 cut(s)	2690
PspFI	CCCAGC	2 cut(s)	749, 1693
PspGI	CCWGG	8 cut(s)	141, 523, 787, 1637, 1869, 1962, 2142, 2640
PspN4I	GGNNCC	7 cut(s)	122, 433, 466, 756, 1031, 1032, 1191
PspOMI	GGGCCC	2 cut(s)	464, 1030
PspPI	GGNCC	8 cut(s)	464, 465, 635, 1030, 1031, 1190, 1573, 2140
PsrI	GAACNNNNNNTAC	2 cut(s)	1376, 1408
PstI	CTGCAG	1 cut(s)	654
PstNI	CAGNNNCTG	2 cut(s)	1316, 2459
PsuI	RGATCY	4 cut(s)	595, 931, 1117, 2104
RsaI	GTAC	7 cut(s)	179, 383, 1214, 1394, 2072, 2288, 2295
RsaNI	GTAC	7 cut(s)	178, 382, 1213, 1393, 2071, 2287, 2294
RseI	CAYNNNNRTG	1 cut(s)	1596
SacI	GAGCTC	1 cut(s)	217
Sau96I	GGNCC	8 cut(s)	464, 465, 635, 1030, 1031, 1190, 1573, 2140
ScaI	AGTACT	1 cut(s)	179
SchI	GAGTC	4 cut(s)	435, 2403, 2426, 2450
ScrFI	CCNGG	8 cut(s)	143, 525, 789, 1639, 1871, 1964, 2144, 2642
SduI	GDGCHC	7 cut(s)	123, 217, 468, 1034, 1417, 2255, 2407
SexAI	ACCWGGT	1 cut(s)	1962
SfaNI	GCATC	7 cut(s)	558, 591, 772, 793, 902, 1051, 2726
SfcI	CTRYAG	1 cut(s)	650
SfuI	TTCGAA	1 cut(s)	538
SinI	GGWCC	3 cut(s)	635, 1190, 1573
SmiMI	CAYNNNNRTG	1 cut(s)	1596
SmlI	CTYRAG	3 cut(s)	1973, 2406, 2565
SmoI	CTYRAG	3 cut(s)	1973, 2406, 2565
SpeI	ACTAGT	1 cut(s)	1046
SphI	GCATGC	3 cut(s)	244, 810, 1717
SseBI	AGGCCT	2 cut(s)	922, 1239
SspI	AATATT	3 cut(s)	676, 1584, 1687
SspMI	CTAG	1 cut(s)	1047
SstI	GAGCTC	1 cut(s)	217
StuI	AGGCCT	2 cut(s)	922, 1239
StyD4I	CCNGG	8 cut(s)	141, 523, 787, 1637, 1869, 1962, 2142, 2640
StyI	CCWWGG	6 cut(s)	113, 758, 1327, 1352, 1404, 1576
TaaI	ACNGT	9 cut(s)	9, 381, 1435, 1531, 1573, 1843, 2228, 2313, 2479
TaiI	ACGT	7 cut(s)	198, 967, 1212, 1389, 1725, 2063, 2720
TaqI	TCGA	2 cut(s)	225, 538
TatI	WGTACW	3 cut(s)	177, 381, 1392
TauI	GCSGC	4 cut(s)	170, 173, 240, 360
TfiI	GAWTC	4 cut(s)	258, 1166, 1517, 2356
TscAI	CASTG	7 cut(s)	85, 375, 442, 1405, 1765, 1778, 1848
TseFI	GTSAC	3 cut(s)	515, 1769, 2690
TseI	GCWGC	9 cut(s)	108, 571, 649, 722, 1127, 1160, 1310, 1789, 2574
Tsp45I	GTSAC	3 cut(s)	515, 1769, 2690
TspDTI	ATGAA	8 cut(s)	678, 1089, 1235, 1314, 1474, 2028, 2481, 2604
TspRI	CASTG	7 cut(s)	85, 375, 442, 1405, 1765, 1778, 1848
Van91I	CCANNNNNTGG	1 cut(s)	2201
Vha464I	CTTAAG	1 cut(s)	1973
VneI	GTGCAC	1 cut(s)	1413
VpaK11BI	GGWCC	3 cut(s)	635, 1190, 1573
XapI	RAATTY	5 cut(s)	421, 841, 1222, 1945, 2543
XceI	RCATGY	4 cut(s)	244, 810, 1127, 1717
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1548, 2638
XmnI	GAANNNNTTC	1 cut(s)	2444
XspI	CTAG	1 cut(s)	1047
ZrmI	AGTACT	1 cut(s)	179
Zsp2I	ATGCAT	3 cut(s)	349, 808, 895

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.