Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0014328

Physical Location & Seq

Genomic Coordinates Forward (+)

17081348 .. 17096168

Gene Structure 14821 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g13490.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2775 bp

ATGCGTAACAGATGCGTCCAACAACCGGATAGCTACAACGTCTCTATCCTCCATTTTCCGTCTTTGCTTTCTCCGATACTTCCCTCTCTCTCTCTCTCTCTCAGTCTCTCTCACCAAGTCACTATGCTGCTCCGGCTCCGGCTCCGATGCCTCTTTCTCTGCCTCACTATTCACTTCGCTCTAGCCGCCGCCGCCTTCTTCAAGCCCTTCAACGTCAGCTACGACCACCGCGCTCTCATCATCGATGGCCGCCGCCGTATGCTCGTCTCCGCCGGCATCCACTACCCCCGCGCCACTCCCGAGATGTGGTCTGATTTGATTGCGAAGAGCAAGGAAGGCGGGGCGGATGTGATCCAGACCTATGCGTTTTGGAGCGGCCACGAGCCGCAGAGAGGACAGTACTACTTTGGAGGGAGATATGATATCGTCAAGTTTGTCAAGTTAGTGGGAGCCAGTGGGCTCTATTTCCATCTTCGCATAGGGCCTTACGTCTGCGCTGAGTGGAACTTTGGTGGCTTCCCAGTGTGGCTGCGGGACATACCTGGAATAGAATTTCGAACAGACAATGCACCTTTTAAGGAAGAGATGCAGCGGTTTGTTAAAAAGATGGTGGATCTGATGCGAGAGGAAGAGCTATTTTCTTGGCAAGGTGGTCCAGTAATTATGCTACAGATTGAAAATGAATATGGAAATATCGAAAGCTCATTTGGCCAGAGAGGGAAGGAATATGTTAAGTGGGCTGCTAACATGGCTCTAGGTCTCGGTGCTGGGGTCCCATGGGTCATGTGCAGGCAAGTTGATGCTCCAGGAAGTATTATAGATGCATGCAATGGATACTACTGTGATGGATACAAGTCAAATTCGTATAACAAGCCAATTCTATGGACAGAAGATTGGGACGGATGGTATGCAACATGGGGTGGAAGATTGCCTCACAGGCCTGTTGAAGATCTTGCATTTGCCGTAGCACGCTTTTACCAGCGTGGAGGAAGCTTTCAGAATTATTATATGTATTTTGGTGGTACAAACTTTGGGCGGACTTCTGGGGGGCCATTCTATATTACCAGTTATGATTATGATGCTCCAATTGATGAATATGGACTGCTGAGTGAACCTAAATGGGGACATCTGAAGGATCTACATGCTGCTATAAAGCTTTGTGAGCCTGCTCTTGTTGCTGCTGATTCACCGCATTACATCAAATTGGGACCAAAACAGGAGGCACATGTATATGGCACAACAGTTCTCACTAAAGGCTTAAACTTTACTTGGATTGGTAGAAGTCAAAGCCAGTGCTCCGCGTTCCTGGCAAACATTGATGAGCATAAATCAGCCTCTGTAACATTCCTTGGTCAAAAATATAATCTCCCACCATGGTCGGTCAGTATCTTGCCAGACTGTAGGAATGTTGTGTTCAACACTGCCAAGGTTGGGACGCAGATTTCCATAAAAAGAGTAAACTTTGATTTGCCTCTTTCTTCAGTTATTTCCGATAACAAAATTTTGCATATCACAAAACCCTGGATGACTGTGGAAGAGCCAATTGGTATTTGGAGTGAGAACAAATTCACAGCCCCCGGAATATTGGAGCATTTAAATGTGACAAAGGATCATTCTGATTACCTATGGCATCTAACCAGAATATTTGTTTCAGATGAAGACATCTCTTTTTGGGAGGAAAACAAGATTAGTCCAGCAATTACGATTGATAGCATGCGTGATGTGTTGCGTGTTTTTGTTAATGGCCAGCTTACAGGCAGTGTGATTGGTCATTGGGTCAAAGTAGTGCAGCCTGTTAGGTTTGTCAAGGGATATAATGACTTGTTGCTGTTAACTCAGACAGTTGGCTTGCAGAACTATGGCGCTTTCCTTGAGAAAGACGGGGCAGGATTTAGAGGACATGTCAAACTTACTGGGTTCAAAAATGGGGATATCGACCTCTCAACATTGTTATGGACTTACCAGGTTGGGCTTAAAGGAGAGTTTTTCAAAATATACACCATAGAAGAAAATGAGAAGGCAGGCTGGGCTGAGTTGTCAGTAGATGCTGATCCATCCACTTTTACCTGGTACAAGACATACTTTGATAGCCCTGCTGGCACAGATCCAGTTGCTCTAAATTTAGGAAGCATGGGAAAAGGCCAGGCTTGGGTCAATGGTCACCATATAGGAAGATATTGGACACTTGTTGCTCCAAAAGATGGATGCCAAGAAATATGTGATTATCGAGGAGCATACAACTCTGATAAGTGCTCAACTAACTGCGGGAAGCCTACTCAAACCTGGTACCATATTCCACGTTCATGGCTGCAGGCTTCAAGTAATTTACTTGTAATCTTTGAGGAAACTGGAGGGAATCCATTCGAGATATCAATCAACTCACGTGCAGTTGGAACCATCTGTGCTCAAGTATCAGAGTCTCACTATCCACCTGTCCAGAAGTGGTTCAATCCAGATTTTATTGATAAAAAAGGCAGAGTAAATGATCGGACACCAGAAATTCACTTGCATTGTCAAGACGGGTTTATAATATCTTCGATAGAATTTGCTAGTTATGGAAGTCCTCAAGGTCATTGCCAGAAGTTTTCTCAAGGGAATTGCCATGCTTCGAATTCCTTCTCCATAGTTTCTGAGGCTTGCCTGGGAAAGAATAACTGTTCAATTGGAGTATCAAATCTTGTATTCGGAGGTGACCCATGCCGGGGTATTGTTAAGACGCTAGCTATTGAGGCAAGCTGCAGGTCACTACCAACTGTTGGTTTTTCTCAGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0009505 GO:0015925 GO:0016020 GO:0016787 GO:0016798 GO:0030312 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

925

Amino Acids

103.77

Weight (kDa)

6.83

Isoelectric Point (pI)

40.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	79 - 383	2.6e-114	Glycosyl hydrolases family 35
GHD	PF17834	428 - 477	2.1e-24	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	689 - 775	1.6e-25	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	836 - 913	2.3e-22	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014328)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32810 AT2G32810
fragaria_vesca	FvH4_4g13490
malus_domestica	MD13G1283100.v1.1
prunus_persica	Prupe.1G160600_v2.0.a1 Prupe.1G160600_v2.0.a1
pyrus_communis	pycom420g01190
rosa_chinensis	RchiOBHm_Chr4g0414111
rosa_laevigata	RLG00000008175
rosa_multiflora	Rmu_sc0003264.1_g000008
rosa_roxburghii	Rroxscaffold_5G00358040
rosa_rugosa	Rorug04G0125600 Rorug04G0125700
rosa_samantha	Rh4AG184200 Rh4BG183700 Rh4DG179600
rosa_wichuraiana	Rw4G015680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2528
Acc36I	ACCTGC	1 cut(s)	2730
Acc65I	GGTACC	1 cut(s)	2286
AccB1I	GGYRCC	1 cut(s)	2286
AccB7I	CCANNNNNTGG	2 cut(s)	2201, 2756
AccBSI	CCGCTC	1 cut(s)	375
AccII	CGCG	3 cut(s)	231, 291, 1301
AclWI	GGATC	6 cut(s)	346, 621, 1143, 1617, 2045, 2099
AcoI	YGGCCR	4 cut(s)	247, 376, 709, 1744
AcsI	RAATTY	8 cut(s)	551, 859, 1500, 1565, 2119, 2499, 2543, 2611
AcuI	CTGAAG	2 cut(s)	1151, 1464
AcvI	CACGTG	1 cut(s)	2384
AfaI	GTAC	4 cut(s)	401, 1024, 2072, 2288
AflIII	ACRYGT	2 cut(s)	1225, 1900
AjnI	CCWGG	9 cut(s)	541, 805, 1305, 1520, 1962, 2066, 2142, 2282, 2640
AjuI	GAANNNNNNNTTGG	4 cut(s)	690, 722, 1527, 1559
Alw21I	GWGCWC	3 cut(s)	1298, 2255, 2407
Alw26I	GTCTC	5 cut(s)	46, 110, 271, 764, 2424
AlwI	GGATC	6 cut(s)	346, 621, 1143, 1617, 2045, 2099
AlwNI	CAGNNNCTG	1 cut(s)	1337
Ama87I	CYCGRG	1 cut(s)	299
AoxI	GGCC	8 cut(s)	247, 376, 482, 709, 938, 1049, 1744, 2140
ApeKI	GCWGC	9 cut(s)	127, 529, 589, 740, 1145, 1178, 1789, 2308, 2736
ApoI	RAATTY	8 cut(s)	551, 859, 1500, 1565, 2119, 2499, 2543, 2611
Asp700I	GAANNNNTTC	4 cut(s)	1565, 2360, 2444, 2615
Asp718I	GGTACC	1 cut(s)	2286
AspLEI	GCGC	4 cut(s)	233, 293, 497, 1865
AspS9I	GGNCC	5 cut(s)	482, 653, 772, 1049, 1208
AsuC2I	CCSGG	2 cut(s)	1578, 2702
AsuHPI	GGTGA	4 cut(s)	104, 1179, 2153, 2702
AsuII	TTCGAA	2 cut(s)	556, 2609
AsuNHI	GCTAGC	1 cut(s)	2719
AvaI	CYCGRG	1 cut(s)	299
AvaII	GGWCC	3 cut(s)	653, 772, 1208
BaeI	ACNNNNGTAYC	2 cut(s)	826, 859
BalI	TGGCCA	2 cut(s)	711, 1746
BanI	GGYRCC	1 cut(s)	2286
BanII	GRGCYC	1 cut(s)	462
BauI	CACGAG	1 cut(s)	380
BbrPI	CACGTG	1 cut(s)	2384
BbsI	GAAGAC	1 cut(s)	1665
Bbv12I	GWGCWC	3 cut(s)	1298, 2255, 2407
BbvI	GCAGC	9 cut(s)	114, 516, 601, 727, 1132, 1165, 1801, 2295, 2723
BccI	CCATC	8 cut(s)	239, 477, 601, 839, 897, 2062, 2195, 2405
BceAI	ACGGC	2 cut(s)	240, 947
BciT130I	CCWGG	9 cut(s)	543, 807, 1307, 1522, 1964, 2068, 2144, 2284, 2642
BciVI	GTATCC	2 cut(s)	827, 842
BcnI	CCSGG	2 cut(s)	1578, 2702
BcoDI	GTCTC	5 cut(s)	46, 110, 271, 764, 2424
BfaI	CTAG	4 cut(s)	182, 755, 2550, 2720
BfmI	CTRYAG	4 cut(s)	668, 1399, 2309, 2737
BfoI	RGCGCY	1 cut(s)	1866
BfuAI	ACCTGC	1 cut(s)	2730
BfuI	GTATCC	2 cut(s)	827, 842
BglI	GCCNNNNNGGC	2 cut(s)	937, 2097
BglII	AGATCT	1 cut(s)	949
BmcAI	AGTACT	1 cut(s)	401
Bme18I	GGWCC	3 cut(s)	653, 772, 1208
BmeT110I	CYCGRG	1 cut(s)	299
BmgT120I	GGNCC	5 cut(s)	482, 653, 772, 1049, 1208
BmiI	GGNNCC	9 cut(s)	137, 143, 451, 773, 774, 1050, 1209, 2288, 2395
BmrI	ACTGGG	2 cut(s)	515, 1923
BmtI	GCTAGC	1 cut(s)	2723
BmuI	ACTGGG	2 cut(s)	515, 1923
BpiI	GAAGAC	1 cut(s)	1665
BpmI	CTGGAG	2 cut(s)	789, 2370
Bpu14I	TTCGAA	2 cut(s)	556, 2609
BpuEI	CTTGAG	4 cut(s)	1892, 2391, 2550, 2574
BpuMI	CCSGG	2 cut(s)	1578, 2702
Bsa29I	ATCGAT	1 cut(s)	243
BsaAI	YACGTR	1 cut(s)	2384
BsaBI	GATNNNNATC	2 cut(s)	2049, 2372
BsaI	GGTCTC	1 cut(s)	764
BsaJI	CCNNGG	8 cut(s)	776, 1348, 1373, 1425, 1520, 1576, 2641, 2701
BsaWI	WCCGGW	1 cut(s)	25
Bse118I	RCCGGY	1 cut(s)	272
Bse1I	ACTGG	8 cut(s)	453, 521, 656, 1065, 1291, 1918, 2108, 2353
Bse3DI	GCAATG	2 cut(s)	835, 2572
Bse8I	GATNNNNATC	2 cut(s)	2049, 2372
BseBI	CCWGG	9 cut(s)	543, 807, 1307, 1522, 1964, 2068, 2144, 2284, 2642
BseCI	ATCGAT	1 cut(s)	243
BseDI	CCNNGG	8 cut(s)	776, 1348, 1373, 1425, 1520, 1576, 2641, 2701
BseGI	GGATG	6 cut(s)	276, 352, 908, 1530, 2054, 2210
BseJI	GATNNNNATC	2 cut(s)	2049, 2372
BseMI	GCAATG	2 cut(s)	835, 2572
BseMII	CTCAG	6 cut(s)	115, 489, 1097, 1850, 2022, 2622
BseNI	ACTGG	8 cut(s)	453, 521, 656, 1065, 1291, 1918, 2108, 2353
BseRI	GAGGAG	1 cut(s)	2244
BseXI	GCAGC	9 cut(s)	114, 516, 601, 727, 1132, 1165, 1801, 2295, 2723
BseYI	CCCAGC	2 cut(s)	767, 2025
BsgI	GTGCAG	3 cut(s)	808, 1808, 2406
Bsh1236I	CGCG	3 cut(s)	231, 291, 1301
BshFI	GGCC	8 cut(s)	249, 378, 484, 711, 940, 1051, 1746, 2142
BshNI	GGYRCC	1 cut(s)	2286
BshVI	ATCGAT	1 cut(s)	243
BsiHKAI	GWGCWC	3 cut(s)	1298, 2255, 2407
BsiHKCI	CYCGRG	1 cut(s)	299
BsiSI	CCGG	6 cut(s)	26, 133, 139, 273, 1578, 2701
BslFI	GGGAC	6 cut(s)	548, 758, 911, 1137, 1221, 1447
BsmAI	GTCTC	5 cut(s)	46, 110, 271, 764, 2424
BsmBI	CGTCTC	2 cut(s)	46, 271
BsmFI	GGGAC	6 cut(s)	548, 758, 911, 1137, 1221, 1447
BsnI	GGCC	8 cut(s)	249, 378, 484, 711, 940, 1051, 1746, 2142
Bso31I	GGTCTC	1 cut(s)	764
BsoBI	CYCGRG	1 cut(s)	299
Bsp119I	TTCGAA	2 cut(s)	556, 2609
Bsp1286I	GDGCHC	4 cut(s)	462, 1298, 2255, 2407
Bsp143I	GATC	8 cut(s)	351, 613, 949, 1135, 1609, 2050, 2104, 2485
Bsp19I	CCATGG	2 cut(s)	776, 1373
BspANI	GGCC	8 cut(s)	249, 378, 484, 711, 940, 1051, 1746, 2142
BspCNI	CTCAG	6 cut(s)	114, 490, 1098, 1849, 2023, 2623
BspDI	ATCGAT	1 cut(s)	243
BspFNI	CGCG	3 cut(s)	231, 291, 1301
BspLI	GGNNCC	9 cut(s)	137, 143, 451, 773, 774, 1050, 1209, 2288, 2395
BspMAI	CTGCAG	2 cut(s)	2313, 2741
BspMI	ACCTGC	1 cut(s)	2730
BspOI	GCTAGC	1 cut(s)	2723
BspPI	GGATC	6 cut(s)	346, 621, 1143, 1617, 2045, 2099
BspQI	GCTCTTC	3 cut(s)	320, 624, 1530
BspT104I	TTCGAA	2 cut(s)	556, 2609
BspT107I	GGYRCC	1 cut(s)	2286
BspTNI	GGTCTC	1 cut(s)	764
BsrBI	CCGCTC	1 cut(s)	375
BsrDI	GCAATG	2 cut(s)	835, 2572
BsrFI	RCCGGY	1 cut(s)	272
BsrI	ACTGG	8 cut(s)	453, 521, 656, 1065, 1291, 1918, 2108, 2353
BssAI	RCCGGY	1 cut(s)	272
BssECI	CCNNGG	8 cut(s)	776, 1348, 1373, 1425, 1520, 1576, 2641, 2701
BssMI	GATC	8 cut(s)	351, 613, 949, 1135, 1609, 2050, 2104, 2485
BssSI	CACGAG	1 cut(s)	380
BssT1I	CCWWGG	4 cut(s)	776, 1348, 1373, 1425
Bst2BI	CACGAG	1 cut(s)	380
Bst2UI	CCWGG	9 cut(s)	543, 807, 1307, 1522, 1964, 2068, 2144, 2284, 2642
Bst4CI	ACNGT	8 cut(s)	399, 842, 1243, 1400, 1531, 1843, 2657, 2755
Bst6I	CTCTTC	4 cut(s)	320, 576, 624, 1530
BstBAI	YACGTR	1 cut(s)	2384
BstBI	TTCGAA	2 cut(s)	556, 2609
BstDEI	CTNAG	7 cut(s)	101, 498, 1106, 1836, 2031, 2631, 2766
BstDSI	CCRYGG	2 cut(s)	776, 1373
BstEII	GGTNACC	2 cut(s)	2159, 2690
BstF5I	GGATG	6 cut(s)	276, 352, 908, 1530, 2054, 2210
BstFNI	CGCG	3 cut(s)	231, 291, 1301
BstH2I	RGCGCY	1 cut(s)	1866
BstHHI	GCGC	4 cut(s)	233, 293, 497, 1865
BstKTI	GATC	8 cut(s)	354, 616, 952, 1138, 1612, 2053, 2107, 2488
BstMAI	GTCTC	5 cut(s)	46, 110, 271, 764, 2424
BstMBI	GATC	8 cut(s)	351, 613, 949, 1135, 1609, 2050, 2104, 2485
BstNI	CCWGG	9 cut(s)	543, 807, 1307, 1522, 1964, 2068, 2144, 2284, 2642
BstNSI	RCATGY	5 cut(s)	828, 1145, 1229, 1717, 1904
BstPI	GGTNACC	2 cut(s)	2159, 2690
BstSFI	CTRYAG	4 cut(s)	668, 1399, 2309, 2737
BstUI	CGCG	3 cut(s)	231, 291, 1301
BstV1I	GCAGC	9 cut(s)	114, 516, 601, 727, 1132, 1165, 1801, 2295, 2723
BstV2I	GAAGAC	1 cut(s)	1665
BstX2I	RGATCY	4 cut(s)	613, 949, 1135, 2104
BstXI	CCANNNNNNTGG	2 cut(s)	882, 2304
BstYI	RGATCY	4 cut(s)	613, 949, 1135, 2104
Bsu15I	ATCGAT	1 cut(s)	243
BsuI	GTATCC	2 cut(s)	827, 842
BsuRI	GGCC	8 cut(s)	249, 378, 484, 711, 940, 1051, 1746, 2142
BsuTUI	ATCGAT	1 cut(s)	243
BtgI	CCRYGG	2 cut(s)	776, 1373
BtsCI	GGATG	6 cut(s)	276, 352, 908, 1530, 2054, 2210
BtsI	GCAGTG	2 cut(s)	1419, 1765
BtsIMutI	CAGTG	5 cut(s)	460, 528, 1298, 1419, 1765
BveI	ACCTGC	1 cut(s)	2730
CaiI	CAGNNNCTG	1 cut(s)	1337
CfoI	GCGC	4 cut(s)	233, 293, 497, 1865
Cfr10I	RCCGGY	1 cut(s)	272
Cfr13I	GGNCC	5 cut(s)	482, 653, 772, 1049, 1208
ClaI	ATCGAT	1 cut(s)	243
CseI	GACGC	3 cut(s)	4, 1444, 2725
CsiI	ACCWGGT	3 cut(s)	1962, 2066, 2282
Csp6I	GTAC	4 cut(s)	400, 1023, 2071, 2287
CviQI	GTAC	4 cut(s)	400, 1023, 2071, 2287
DdeI	CTNAG	7 cut(s)	101, 498, 1106, 1836, 2031, 2631, 2766
DpnI	GATC	8 cut(s)	353, 615, 951, 1137, 1611, 2052, 2106, 2487
DpnII	GATC	8 cut(s)	351, 613, 949, 1135, 1609, 2050, 2104, 2485
DraI	TTTAAA	1 cut(s)	1596
EaeI	YGGCCR	4 cut(s)	247, 376, 709, 1744
Eam1104I	CTCTTC	4 cut(s)	320, 576, 624, 1530
EarI	CTCTTC	4 cut(s)	320, 576, 624, 1530
EciI	GGCGGA	3 cut(s)	259, 359, 1051
Eco130I	CCWWGG	4 cut(s)	776, 1348, 1373, 1425
Eco147I	AGGCCT	1 cut(s)	940
Eco24I	GRGCYC	1 cut(s)	462
Eco31I	GGTCTC	1 cut(s)	764
Eco32I	GATATC	3 cut(s)	424, 1933, 2370
Eco47I	GGWCC	3 cut(s)	653, 772, 1208
Eco57I	CTGAAG	2 cut(s)	1151, 1464
Eco72I	CACGTG	1 cut(s)	2384
Eco88I	CYCGRG	1 cut(s)	299
Eco91I	GGTNACC	2 cut(s)	2159, 2690
EcoO109I	RGGNCCY	2 cut(s)	482, 772
EcoO65I	GGTNACC	2 cut(s)	2159, 2690
EcoRI	GAATTC	1 cut(s)	2611
EcoRII	CCWGG	9 cut(s)	541, 805, 1305, 1520, 1962, 2066, 2142, 2282, 2640
EcoRV	GATATC	3 cut(s)	424, 1933, 2370
EcoT14I	CCWWGG	4 cut(s)	776, 1348, 1373, 1425
EcoT22I	ATGCAT	1 cut(s)	826
EcoT38I	GRGCYC	1 cut(s)	462
ErhI	CCWWGG	4 cut(s)	776, 1348, 1373, 1425
Esp3I	CGTCTC	2 cut(s)	46, 271
FalI	AAGNNNNNCTT	2 cut(s)	2066, 2098
FaqI	GGGAC	6 cut(s)	548, 758, 911, 1137, 1221, 1447
FauI	CCCGC	4 cut(s)	296, 332, 525, 2258
FokI	GGATG	6 cut(s)	263, 359, 915, 1537, 2041, 2217
FriOI	GRGCYC	1 cut(s)	462
FspBI	CTAG	4 cut(s)	182, 755, 2550, 2720
GlaI	GCGC	4 cut(s)	232, 292, 496, 1864
GsaI	CCCAGC	2 cut(s)	771, 2029
GsuI	CTGGAG	2 cut(s)	789, 2370
HaeII	RGCGCY	1 cut(s)	1866
HaeIII	GGCC	8 cut(s)	249, 378, 484, 711, 940, 1051, 1746, 2142
HapII	CCGG	6 cut(s)	26, 133, 139, 273, 1578, 2701
HgaI	GACGC	3 cut(s)	4, 1444, 2725
HhaI	GCGC	4 cut(s)	233, 293, 497, 1865
Hin6I	GCGC	4 cut(s)	231, 291, 495, 1863
HinP1I	GCGC	4 cut(s)	231, 291, 495, 1863
HincII	GTYRAC	1 cut(s)	1833
HindII	GTYRAC	1 cut(s)	1833
HindIII	AAGCTT	2 cut(s)	991, 1154
HinfI	GANTC	3 cut(s)	1184, 2356, 2417
HpaI	GTTAAC	1 cut(s)	1833
HpaII	CCGG	6 cut(s)	26, 133, 139, 273, 1578, 2701
HphI	GGTGA	4 cut(s)	104, 1179, 2153, 2702
Hpy166II	GTNNAC	3 cut(s)	1112, 1459, 1833
Hpy188III	TCNNGA	6 cut(s)	299, 355, 2365, 2437, 2453, 2516
Hpy8I	GTNNAC	3 cut(s)	1112, 1459, 1833
HpyAV	CCTTC	7 cut(s)	205, 217, 329, 715, 1126, 2011, 2626
HpyCH4III	ACNGT	8 cut(s)	399, 842, 1243, 1400, 1531, 1843, 2657, 2755
HpyCH4IV	ACGT	5 cut(s)	39, 213, 489, 2299, 2383
HpyF3I	CTNAG	7 cut(s)	101, 498, 1106, 1836, 2031, 2631, 2766
HpySE526I	ACGT	5 cut(s)	39, 213, 489, 2299, 2383
HspAI	GCGC	4 cut(s)	231, 291, 495, 1863
KflI	GGGWCCC	1 cut(s)	772
KpnI	GGTACC	1 cut(s)	2290
KroI	GCCGGC	1 cut(s)	272
KroNI	GCCGGC	1 cut(s)	274
KspAI	GTTAAC	1 cut(s)	1833
Kzo9I	GATC	8 cut(s)	351, 613, 949, 1135, 1609, 2050, 2104, 2485
LguI	GCTCTTC	3 cut(s)	320, 624, 1530
Lsp1109I	GCAGC	9 cut(s)	114, 516, 601, 727, 1132, 1165, 1801, 2295, 2723
MabI	ACCWGGT	3 cut(s)	1962, 2066, 2282
MaeI	CTAG	4 cut(s)	182, 755, 2550, 2720
MaeII	ACGT	5 cut(s)	39, 213, 489, 2299, 2383
MaeIII	GTNAC	7 cut(s)	5, 118, 1339, 1600, 2159, 2690, 2742
MalI	GATC	8 cut(s)	353, 615, 951, 1137, 1611, 2052, 2106, 2487
MbiI	CCGCTC	1 cut(s)	375
MboI	GATC	8 cut(s)	351, 613, 949, 1135, 1609, 2050, 2104, 2485
MfeI	CAATTG	3 cut(s)	1086, 1542, 2661
MflI	RGATCY	4 cut(s)	613, 949, 1135, 2104
MhlI	GDGCHC	4 cut(s)	462, 1298, 2255, 2407
MlsI	TGGCCA	2 cut(s)	711, 1746
MluNI	TGGCCA	2 cut(s)	711, 1746
MlyI	GAGTC	1 cut(s)	2426
MmeI	TCCRAC	2 cut(s)	43, 2371
Mox20I	TGGCCA	2 cut(s)	711, 1746
Mph1103I	ATGCAT	1 cut(s)	826
MroNI	GCCGGC	1 cut(s)	272
MroXI	GAANNNNTTC	4 cut(s)	1565, 2360, 2444, 2615
MscI	TGGCCA	2 cut(s)	711, 1746
MseI	TTAA	9 cut(s)	576, 600, 732, 1259, 1595, 1740, 1832, 1974, 2712
MslI	CAYNNNNRTG	4 cut(s)	1230, 1596, 1951, 2302
Msp20I	TGGCCA	2 cut(s)	711, 1746
MspA1I	CMGCKG	1 cut(s)	592
MspI	CCGG	6 cut(s)	26, 133, 139, 273, 1578, 2701
MunI	CAATTG	3 cut(s)	1086, 1542, 2661
MvaI	CCWGG	9 cut(s)	543, 807, 1307, 1522, 1964, 2068, 2144, 2284, 2642
MvnI	CGCG	3 cut(s)	231, 291, 1301
NaeI	GCCGGC	1 cut(s)	274
NciI	CCSGG	2 cut(s)	1578, 2702
NcoI	CCATGG	2 cut(s)	776, 1373
NdeII	GATC	8 cut(s)	351, 613, 949, 1135, 1609, 2050, 2104, 2485
NgoMIV	GCCGGC	1 cut(s)	272
NheI	GCTAGC	1 cut(s)	2719
NlaIV	GGNNCC	9 cut(s)	137, 143, 451, 773, 774, 1050, 1209, 2288, 2395
NmuCI	GTSAC	5 cut(s)	118, 1600, 2159, 2690, 2742
NsiI	ATGCAT	1 cut(s)	826
NspI	RCATGY	5 cut(s)	828, 1145, 1229, 1717, 1904
NspV	TTCGAA	2 cut(s)	556, 2609
PaeI	GCATGC	2 cut(s)	828, 1717
PceI	AGGCCT	1 cut(s)	940
PciI	ACATGT	2 cut(s)	1225, 1900
PciSI	GCTCTTC	3 cut(s)	320, 624, 1530
PcsI	WCGNNNNNNNCGW	1 cut(s)	219
PdiI	GCCGGC	1 cut(s)	274
PdmI	GAANNNNTTC	4 cut(s)	1565, 2360, 2444, 2615
PfeI	GAWTC	2 cut(s)	1184, 2356
PflMI	CCANNNNNTGG	2 cut(s)	2201, 2756
PfoI	TCCNGGA	1 cut(s)	805
PleI	GAGTC	1 cut(s)	2425
PmaCI	CACGTG	1 cut(s)	2384
PmlI	CACGTG	1 cut(s)	2384
PpsI	GAGTC	1 cut(s)	2425
Ppu21I	YACGTR	1 cut(s)	2384
PpuMI	RGGWCCY	1 cut(s)	772
PscI	ACATGT	2 cut(s)	1225, 1900
PsiI	TTATAA	1 cut(s)	2528
Psp5II	RGGWCCY	1 cut(s)	772
Psp6I	CCWGG	9 cut(s)	541, 805, 1305, 1520, 1962, 2066, 2142, 2282, 2640
PspCI	CACGTG	1 cut(s)	2384
PspEI	GGTNACC	2 cut(s)	2159, 2690
PspFI	CCCAGC	2 cut(s)	767, 2025
PspGI	CCWGG	9 cut(s)	541, 805, 1305, 1520, 1962, 2066, 2142, 2282, 2640
PspN4I	GGNNCC	9 cut(s)	137, 143, 451, 773, 774, 1050, 1209, 2288, 2395
PspPI	GGNCC	5 cut(s)	482, 653, 772, 1049, 1208
PspPPI	RGGWCCY	1 cut(s)	772
PstI	CTGCAG	2 cut(s)	2313, 2741
PstNI	CAGNNNCTG	1 cut(s)	1337
PsuI	RGATCY	4 cut(s)	613, 949, 1135, 2104
RsaI	GTAC	4 cut(s)	401, 1024, 2072, 2288
RsaNI	GTAC	4 cut(s)	400, 1023, 2071, 2287
RseI	CAYNNNNRTG	4 cut(s)	1230, 1596, 1951, 2302
SapI	GCTCTTC	3 cut(s)	320, 624, 1530
SaqAI	TTAA	9 cut(s)	576, 600, 732, 1259, 1595, 1740, 1832, 1974, 2712
Sau3AI	GATC	8 cut(s)	351, 613, 949, 1135, 1609, 2050, 2104, 2485
Sau96I	GGNCC	5 cut(s)	482, 653, 772, 1049, 1208
ScaI	AGTACT	1 cut(s)	401
SchI	GAGTC	1 cut(s)	2426
SduI	GDGCHC	4 cut(s)	462, 1298, 2255, 2407
SexAI	ACCWGGT	3 cut(s)	1962, 2066, 2282
SfcI	CTRYAG	4 cut(s)	668, 1399, 2309, 2737
SfuI	TTCGAA	2 cut(s)	556, 2609
SinI	GGWCC	3 cut(s)	653, 772, 1208
SmiI	ATTTAAAT	1 cut(s)	1596
SmiMI	CAYNNNNRTG	4 cut(s)	1230, 1596, 1951, 2302
SmlI	CTYRAG	4 cut(s)	1871, 2406, 2565, 2589
SmoI	CTYRAG	4 cut(s)	1871, 2406, 2565, 2589
SphI	GCATGC	2 cut(s)	828, 1717
SseBI	AGGCCT	1 cut(s)	940
SspI	AATATT	2 cut(s)	1584, 1644
SspMI	CTAG	4 cut(s)	182, 755, 2550, 2720
StuI	AGGCCT	1 cut(s)	940
StyI	CCWWGG	4 cut(s)	776, 1348, 1373, 1425
SwaI	ATTTAAAT	1 cut(s)	1596
TaaI	ACNGT	8 cut(s)	399, 842, 1243, 1400, 1531, 1843, 2657, 2755
TaiI	ACGT	5 cut(s)	42, 216, 492, 2302, 2386
TaqI	TCGA	8 cut(s)	243, 556, 696, 1935, 2227, 2364, 2537, 2609
TaqII	GACCGA	1 cut(s)	1369
TatI	WGTACW	1 cut(s)	399
TauI	GCSGC	7 cut(s)	188, 191, 194, 252, 255, 378, 388
TfiI	GAWTC	2 cut(s)	1184, 2356
Tru1I	TTAA	9 cut(s)	576, 600, 732, 1259, 1595, 1740, 1832, 1974, 2712
Tru9I	TTAA	9 cut(s)	576, 600, 732, 1259, 1595, 1740, 1832, 1974, 2712
TscAI	CASTG	5 cut(s)	460, 528, 1298, 1426, 1765
TseFI	GTSAC	5 cut(s)	118, 1600, 2159, 2690, 2742
TseI	GCWGC	9 cut(s)	127, 529, 589, 740, 1145, 1178, 1789, 2308, 2736
Tsp45I	GTSAC	5 cut(s)	118, 1600, 2159, 2690, 2742
TspDTI	ATGAA	4 cut(s)	696, 1107, 1671, 2292
TspGWI	ACGGA	2 cut(s)	48, 915
TspRI	CASTG	5 cut(s)	460, 528, 1298, 1426, 1765
Van91I	CCANNNNNTGG	2 cut(s)	2201, 2756
VpaK11BI	GGWCC	3 cut(s)	653, 772, 1208
XapI	RAATTY	8 cut(s)	551, 859, 1500, 1565, 2119, 2499, 2543, 2611
XceI	RCATGY	5 cut(s)	828, 1145, 1229, 1717, 1904
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1548
XmnI	GAANNNNTTC	4 cut(s)	1565, 2360, 2444, 2615
XspI	CTAG	4 cut(s)	182, 755, 2550, 2720
ZrmI	AGTACT	1 cut(s)	401
Zsp2I	ATGCAT	1 cut(s)	826

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.