Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0014328

Physical Location & Seq

Genomic Coordinates Forward (+)

38012308 .. 38026175

Gene Structure 13868 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00358040.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2508 bp

ATGCTCCGGCTCCGATGCCTCCTTCTCTGCCTCACTATCCACTTCGCCGCGGTTGCCGCCGCGTTCTTCAAGCCGTTCAACGTCAGCTACGACCACCGCGCTCTCATCATCGATGGCAACCGCCGCATGCTCATCTCCGCCGGCATCCACTACCCTCGCGCCACTCCTCAGATGTGGCCTGATTTGATTGCAAAGAGCAAGGAAGGCGGGGCGGATGTGATCCAGACCTATGCATTTTGGAGCGGCCACGAGCCGCAGAGAGGACAGTACTATTTTGAAGGGAGATATGATATCGTCAAGTTTGTGAATGGCTTCCCGGTGTGGTTGCGGGACATACCTGGAATAGAATTCCGAACAAACAATGCACCTTTTAAGGAAGAGATGCAGCGGTTTGTTAAAAAGATGGTGGATCTAATGCGAGAGGAAGAGCTGTTTTGTTGGCAAGGGGGTCCAGTAATTATGCTACAGGTTGGCCTAGTTTGGACTGTGGTAGTTATTGAAAATGAATATGGAAATATGGAAAGCTCATATGGACAGAGAGGGAAGGAATATGTTAAGTGGGCTGCTGAGATGGCTCTAGGTCTCGGTGCTGGGGTCCCATGGGTCATGTGCAAGCAAGTTGATGCTCCAGGAAGTATTATAGATGCATGCAATGGATACTACTGTGATGGATACAAGTCAAATTCATATAATAAGCCAATTCTTTGGACAGAAGATTGGGATGGATGGTATGCATCATGGGGTGGAAGATTGCCTCACAGGCCTGTTGAAGATCTTGCATTTGCAGTAGCACGATTTTTCCAACGTGGAGGGAGCTTTCAGAATTATTATATGTATTTTGGTGGTACGAACTTTGGCCGGACTTCTGGGGGGCCATTTTATATTACCAGTTATGATTATGATGCTCCAATTGATGAATATGGACTGCTGAGTGAACCTAAATGGGGACATCTGAAGGATCTGCATGCTGCTATAAAGCTTTGTGAGCCTGCTCTTGTTGCTGCTGATTCACCGCATTACATTAAATTGGGACCAAAACAGGAGGCACATGTATATGGTACAACAGTTCACACTAAAGGCTTAAACTTTACTTGGTTTGGTAGAAGTCAAAGCCAGTGTTCTGCATTCCTCGCAAACATTGATGAGCATAAATCAGCCTCTGTAACATTCCTAGGTCAAAAATATAATCTGCCACCATGGTCAGTCAGTATATTGCCAGACTGTAGGAACGTTGTGTTCAACACTGCCAAGGTTGGGGCGCAAACTTCCATAAAAAGAGTAAACTCTGATTTGCCTCTTCCAGTTATTTCCGAGAACAAAAATTTGTATACCACGGAACCCTGGATGACTTTAGAAGAGCCAATTAGTATTTGGAGTGAGAACAATTTCACAGTCCAAGGAATATTGGAGCATTTAAATGTGACAAAGGACCATTCTGATTACCTGTGGCATATAACCAGAATATTTGTTTCAGATGAGGACATCTCTTTTTGGGAGGAAAGCAATATTAGTCCAGCAATTACAATCGATAGCATGCGAGATGTGTTGCGTGTATTTGTTAATGGCCAGCTTACAGGCAGTGTGATCGGTCATTGGGTCAAGGTAGTGCAGCCTGTTAGGTTTGTCAAGGGATATAATGACTTGTTGCTGTTAACTCAGACAGTTGGCTTGCAGAACTATGGTGCTTTCCTTGAGAAAGATGGGGCAGGATTTAGAGGACATGTCAAACTGACTGGGTTTAAAAATGGGGATATTGACCTCTCAACATTGTTATGGACTTACCAGGTTGGGCTTAAAGGAGAGTTTTTCAAAATATACACCATAGAAGAAAATGACAAGGCAGGTTGGGCTGAGTTGTCGCTAGATGCCGATCCATCCACTTTCACCTGGTACAAGCAGACATACTTTGATAGCCCTGCTGGCACAGATCCAGTTGCTCTAAATTTAGGAAGCATGGGAAAGGGCCAGGCTTGGGTCAATGGCCACCATATAGGAAGATATTGGACACTTGTTGCTCCAAAAGATGGATGCCAAGAAATCTGTGATTATCGAGGGGCATACAACTCCGATAAGTGCTCAACAAATTGTGGGAAGCCTACTCAAACCTGGTACCATATTCCACGGTCATGGTTGCAGGCTTCAAGTAATTTACTTGTTATCTTTGAGGAAATTGAGGGAATCCGTTTGAGATTTCAATCAACTCGCTTGCAGTTGGAACCATCTGTGCTCAAGTGTCAGAGTCTCACTACCCACCTGTTTATAATATCTTCCATAGAATTTGCCAGCTATGGAAGCCCTCAAGGTCGTTGCCAGAAGTTTTCTCAAGGGAACTGCCATGCTTCGAATTCTTTCTCCATAGTTTCTGAGGCTTGCCTGGGAAAGAATAGCTGTTCAGTTGGAGTATCAAATCTTGTATTTGGAGGTGACCCATGCCGGGGTATTGTTAAGACATTGGCTGTTGAGGCAAGATGCAGGTCACTACCAACTGTTGGTTTTTCTCAGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0009505 GO:0015925 GO:0016020 GO:0016787 GO:0016798 GO:0030312 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

835

Amino Acids

94.0

Weight (kDa)

6.41

Isoelectric Point (pI)

40.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	35 - 102	9.8e-25	Glycosyl hydrolases family 35
Glyco_hydro_35	PF01301	104 - 324	1.1e-63	Glycosyl hydrolases family 35
GHD	PF17834	364 - 418	5.6e-25	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	629 - 716	4.1e-23	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	754 - 824	1.3e-20	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014328)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32810 AT2G32810
fragaria_vesca	FvH4_4g13490
malus_domestica	MD13G1283100.v1.1
prunus_persica	Prupe.1G160600_v2.0.a1 Prupe.1G160600_v2.0.a1
pyrus_communis	pycom420g01190
rosa_chinensis	RchiOBHm_Chr4g0414111
rosa_laevigata	RLG00000008175
rosa_multiflora	Rmu_sc0003264.1_g000008
rosa_roxburghii	Rroxscaffold_5G00358040
rosa_rugosa	Rorug04G0125600 Rorug04G0125700
rosa_samantha	Rh4AG184200 Rh4BG183700 Rh4DG179600
rosa_wichuraiana	Rw4G015680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2261
Acc36I	ACCTGC	2 cut(s)	1832, 2463
Acc65I	GGTACC	1 cut(s)	2109
AccB1I	GGYRCC	1 cut(s)	2109
AccB7I	CCANNNNNTGG	2 cut(s)	2024, 2489
AccBSI	CCGCTC	1 cut(s)	243
AccI	GTMKAC	1 cut(s)	1328
AccII	CGCG	4 cut(s)	50, 62, 99, 159
AclI	AACGTT	1 cut(s)	1230
AclWI	GGATC	5 cut(s)	214, 417, 966, 1865, 1922
AcoI	YGGCCR	4 cut(s)	244, 856, 1564, 1981
AcsI	RAATTY	6 cut(s)	347, 682, 1321, 1942, 2276, 2344
AcuI	CTGAAG	1 cut(s)	974
AfaI	GTAC	5 cut(s)	269, 847, 1060, 1892, 2111
AfiI	CCNNNNNNNGG	8 cut(s)	260, 944, 1254, 1972, 2024, 2434, 2435, 2489
AflIII	ACRYGT	2 cut(s)	1048, 1720
AgsI	TTSAA	9 cut(s)	70, 79, 278, 500, 770, 1240, 1810, 2142, 2195
AjnI	CCWGG	8 cut(s)	337, 628, 1340, 1782, 1886, 1965, 2105, 2373
AluBI	AGCT	9 cut(s)	87, 430, 525, 816, 979, 1570, 2286, 2388, 2503
AluI	AGCT	9 cut(s)	87, 430, 525, 816, 979, 1570, 2286, 2388, 2503
Alw21I	GWGCWC	2 cut(s)	2078, 2229
Alw26I	GTCTC	2 cut(s)	587, 2246
AlwI	GGATC	5 cut(s)	214, 417, 966, 1865, 1922
AlwNI	CAGNNNCTG	1 cut(s)	1160
AoxI	GGCC	9 cut(s)	176, 244, 472, 761, 856, 872, 1564, 1963, 1981
ApeKI	GCWGC	5 cut(s)	385, 563, 968, 1001, 1609
ApoI	RAATTY	6 cut(s)	347, 682, 1321, 1942, 2276, 2344
Asp700I	GAANNNNTTC	3 cut(s)	311, 1385, 2348
Asp718I	GGTACC	1 cut(s)	2109
AspA2I	CCTAGG	1 cut(s)	1171
AspLEI	GCGC	3 cut(s)	101, 161, 1261
AspS9I	GGNCC	6 cut(s)	449, 595, 872, 1031, 1429, 1963
AsuC2I	CCSGG	2 cut(s)	317, 2435
AsuHPI	GGTGA	3 cut(s)	1002, 1876, 2435
AsuII	TTCGAA	1 cut(s)	2342
AvaII	GGWCC	4 cut(s)	449, 595, 1031, 1429
AvrII	CCTAGG	1 cut(s)	1171
BaeI	ACNNNNGTAYC	4 cut(s)	649, 682, 1050, 1083
BalI	TGGCCA	2 cut(s)	1566, 1983
BanI	GGYRCC	1 cut(s)	2109
BauI	CACGAG	1 cut(s)	248
Bbv12I	GWGCWC	2 cut(s)	2078, 2229
BbvI	GCAGC	5 cut(s)	397, 550, 955, 988, 1621
BceAI	ACGGC	1 cut(s)	58
BcgI	CGANNNNNNTGC	2 cut(s)	1567, 1601
BciT130I	CCWGG	8 cut(s)	339, 630, 1342, 1784, 1888, 1967, 2107, 2375
BciVI	GTATCC	2 cut(s)	650, 665
BcnI	CCSGG	2 cut(s)	317, 2435
BcoDI	GTCTC	2 cut(s)	587, 2246
BfaI	CTAG	4 cut(s)	476, 578, 1172, 1862
BfmI	CTRYAG	2 cut(s)	464, 1222
BfuAI	ACCTGC	2 cut(s)	1832, 2463
BfuI	GTATCC	2 cut(s)	650, 665
BglI	GCCNNNNNGGC	2 cut(s)	760, 1920
BglII	AGATCT	1 cut(s)	772
BlnI	CCTAGG	1 cut(s)	1171
BmcAI	AGTACT	1 cut(s)	269
Bme18I	GGWCC	4 cut(s)	449, 595, 1031, 1429
BmgT120I	GGNCC	6 cut(s)	449, 595, 872, 1031, 1429, 1963
BmiI	GGNNCC	9 cut(s)	11, 450, 596, 597, 873, 1032, 1338, 2111, 2217
BmrI	ACTGGG	1 cut(s)	1743
BmuI	ACTGGG	1 cut(s)	1743
BplI	GAGNNNNNCTC	2 cut(s)	1469, 1501
BpmI	CTGGAG	1 cut(s)	612
Bpu14I	TTCGAA	1 cut(s)	2342
BpuEI	CTTGAG	4 cut(s)	1712, 2213, 2283, 2307
BpuMI	CCSGG	2 cut(s)	317, 2435
Bsa29I	ATCGAT	2 cut(s)	111, 1527
BsaBI	GATNNNNATC	2 cut(s)	1869, 2194
BsaI	GGTCTC	1 cut(s)	587
Bsc4I	CCNNNNNNNGG	8 cut(s)	260, 944, 1254, 1972, 2024, 2434, 2435, 2489
Bse118I	RCCGGY	1 cut(s)	140
Bse1I	ACTGG	6 cut(s)	452, 888, 1114, 1301, 1738, 1931
Bse3DI	GCAATG	1 cut(s)	658
Bse8I	GATNNNNATC	2 cut(s)	1869, 2194
BseBI	CCWGG	8 cut(s)	339, 630, 1342, 1784, 1888, 1967, 2107, 2375
BseCI	ATCGAT	2 cut(s)	111, 1527
BseGI	GGATG	7 cut(s)	144, 220, 727, 731, 1350, 1874, 2033
BseJI	GATNNNNATC	2 cut(s)	1869, 2194
BseLI	CCNNNNNNNGG	8 cut(s)	260, 944, 1254, 1972, 2024, 2434, 2435, 2489
BseMI	GCAATG	1 cut(s)	658
BseMII	CTCAG	6 cut(s)	182, 558, 920, 1670, 1842, 2355
BseNI	ACTGG	6 cut(s)	452, 888, 1114, 1301, 1738, 1931
BseRI	GAGGAG	1 cut(s)	156
BseXI	GCAGC	5 cut(s)	397, 550, 955, 988, 1621
BseYI	CCCAGC	1 cut(s)	590
BsgI	GTGCAG	1 cut(s)	1628
Bsh1236I	CGCG	4 cut(s)	50, 62, 99, 159
BshFI	GGCC	9 cut(s)	178, 246, 474, 763, 858, 874, 1566, 1965, 1983
BshNI	GGYRCC	1 cut(s)	2109
BshVI	ATCGAT	2 cut(s)	111, 1527
BsiHKAI	GWGCWC	2 cut(s)	2078, 2229
BsiSI	CCGG	5 cut(s)	7, 141, 317, 859, 2434
BslFI	GGGAC	4 cut(s)	344, 581, 960, 1044
BslI	CCNNNNNNNGG	8 cut(s)	260, 944, 1254, 1972, 2024, 2434, 2435, 2489
BsmAI	GTCTC	2 cut(s)	587, 2246
BsmFI	GGGAC	4 cut(s)	344, 581, 960, 1044
BsmI	GAATGC	1 cut(s)	1124
BsnI	GGCC	9 cut(s)	178, 246, 474, 763, 858, 874, 1566, 1965, 1983
Bso31I	GGTCTC	1 cut(s)	587
Bsp119I	TTCGAA	1 cut(s)	2342
Bsp1286I	GDGCHC	2 cut(s)	2078, 2229
Bsp143I	GATC	7 cut(s)	219, 409, 772, 958, 1584, 1870, 1927
Bsp19I	CCATGG	2 cut(s)	599, 1196
BspANI	GGCC	9 cut(s)	178, 246, 474, 763, 858, 874, 1566, 1965, 1983
BspCNI	CTCAG	6 cut(s)	181, 559, 921, 1669, 1843, 2356
BspDI	ATCGAT	2 cut(s)	111, 1527
BspFNI	CGCG	4 cut(s)	50, 62, 99, 159
BspLI	GGNNCC	9 cut(s)	11, 450, 596, 597, 873, 1032, 1338, 2111, 2217
BspMI	ACCTGC	2 cut(s)	1832, 2463
BspPI	GGATC	5 cut(s)	214, 417, 966, 1865, 1922
BspQI	GCTCTTC	2 cut(s)	420, 1350
BspT104I	TTCGAA	1 cut(s)	2342
BspT107I	GGYRCC	1 cut(s)	2109
BspTNI	GGTCTC	1 cut(s)	587
BsrBI	CCGCTC	1 cut(s)	243
BsrDI	GCAATG	1 cut(s)	658
BsrFI	RCCGGY	1 cut(s)	140
BsrI	ACTGG	6 cut(s)	452, 888, 1114, 1301, 1738, 1931
BssAI	RCCGGY	1 cut(s)	140
BssMI	GATC	7 cut(s)	219, 409, 772, 958, 1584, 1870, 1927
BssNAI	GTATAC	1 cut(s)	1329
BssSI	CACGAG	1 cut(s)	248
BssT1I	CCWWGG	5 cut(s)	599, 1171, 1196, 1248, 1396
Bst1107I	GTATAC	1 cut(s)	1329
Bst2BI	CACGAG	1 cut(s)	248
Bst2UI	CCWGG	8 cut(s)	339, 630, 1342, 1784, 1888, 1967, 2107, 2375
Bst4CI	ACNGT	9 cut(s)	267, 487, 665, 1066, 1223, 1393, 1663, 2124, 2488
Bst6I	CTCTTC	4 cut(s)	372, 420, 1302, 1350
BstBI	TTCGAA	1 cut(s)	2342
BstDEI	CTNAG	7 cut(s)	168, 567, 929, 1656, 1851, 2364, 2499
BstDSI	CCRYGG	5 cut(s)	48, 599, 1196, 1332, 2120
BstEII	GGTNACC	1 cut(s)	2423
BstF5I	GGATG	7 cut(s)	144, 220, 727, 731, 1350, 1874, 2033
BstFNI	CGCG	4 cut(s)	50, 62, 99, 159
BstHHI	GCGC	3 cut(s)	101, 161, 1261
BstKTI	GATC	7 cut(s)	222, 412, 775, 961, 1587, 1873, 1930
BstMAI	GTCTC	2 cut(s)	587, 2246
BstMBI	GATC	7 cut(s)	219, 409, 772, 958, 1584, 1870, 1927
BstNI	CCWGG	8 cut(s)	339, 630, 1342, 1784, 1888, 1967, 2107, 2375
BstNSI	RCATGY	6 cut(s)	130, 651, 968, 1052, 1537, 1724
BstPI	GGTNACC	1 cut(s)	2423
BstSFI	CTRYAG	2 cut(s)	464, 1222
BstUI	CGCG	4 cut(s)	50, 62, 99, 159
BstV1I	GCAGC	5 cut(s)	397, 550, 955, 988, 1621
BstX2I	RGATCY	4 cut(s)	409, 772, 958, 1927
BstXI	CCANNNNNNTGG	2 cut(s)	705, 2127
BstYI	RGATCY	4 cut(s)	409, 772, 958, 1927
BstZ17I	GTATAC	1 cut(s)	1329
Bsu15I	ATCGAT	2 cut(s)	111, 1527
BsuI	GTATCC	2 cut(s)	650, 665
BsuRI	GGCC	9 cut(s)	178, 246, 474, 763, 858, 874, 1566, 1965, 1983
BsuTUI	ATCGAT	2 cut(s)	111, 1527
BtgI	CCRYGG	5 cut(s)	48, 599, 1196, 1332, 2120
BtsCI	GGATG	7 cut(s)	144, 220, 727, 731, 1350, 1874, 2033
BtsI	GCAGTG	2 cut(s)	1242, 1585
BtsIMutI	CAGTG	3 cut(s)	1121, 1242, 1585
BveI	ACCTGC	2 cut(s)	1832, 2463
CaiI	CAGNNNCTG	1 cut(s)	1160
CfoI	GCGC	3 cut(s)	101, 161, 1261
Cfr10I	RCCGGY	1 cut(s)	140
Cfr13I	GGNCC	6 cut(s)	449, 595, 872, 1031, 1429, 1963
Cfr42I	CCGCGG	1 cut(s)	51
ClaI	ATCGAT	2 cut(s)	111, 1527
CsiI	ACCWGGT	3 cut(s)	1782, 1886, 2105
Csp6I	GTAC	5 cut(s)	268, 846, 1059, 1891, 2110
CviQI	GTAC	5 cut(s)	268, 846, 1059, 1891, 2110
DdeI	CTNAG	7 cut(s)	168, 567, 929, 1656, 1851, 2364, 2499
DpnI	GATC	7 cut(s)	221, 411, 774, 960, 1586, 1872, 1929
DpnII	GATC	7 cut(s)	219, 409, 772, 958, 1584, 1870, 1927
DraI	TTTAAA	2 cut(s)	1416, 1741
EaeI	YGGCCR	4 cut(s)	244, 856, 1564, 1981
Eam1104I	CTCTTC	4 cut(s)	372, 420, 1302, 1350
EarI	CTCTTC	4 cut(s)	372, 420, 1302, 1350
EciI	GGCGGA	2 cut(s)	127, 227
Eco130I	CCWWGG	5 cut(s)	599, 1171, 1196, 1248, 1396
Eco147I	AGGCCT	1 cut(s)	763
Eco31I	GGTCTC	1 cut(s)	587
Eco32I	GATATC	1 cut(s)	292
Eco47I	GGWCC	4 cut(s)	449, 595, 1031, 1429
Eco57I	CTGAAG	1 cut(s)	974
Eco91I	GGTNACC	1 cut(s)	2423
EcoO109I	RGGNCCY	1 cut(s)	595
EcoO65I	GGTNACC	1 cut(s)	2423
EcoRI	GAATTC	2 cut(s)	347, 2344
EcoRII	CCWGG	8 cut(s)	337, 628, 1340, 1782, 1886, 1965, 2105, 2373
EcoRV	GATATC	1 cut(s)	292
EcoT14I	CCWWGG	5 cut(s)	599, 1171, 1196, 1248, 1396
EcoT22I	ATGCAT	3 cut(s)	235, 649, 736
ErhI	CCWWGG	5 cut(s)	599, 1171, 1196, 1248, 1396
FaqI	GGGAC	4 cut(s)	344, 581, 960, 1044
FauI	CCCGC	2 cut(s)	200, 321
FauNDI	CATATG	1 cut(s)	529
FblI	GTMKAC	1 cut(s)	1328
FokI	GGATG	7 cut(s)	131, 227, 734, 738, 1357, 1861, 2040
FspBI	CTAG	4 cut(s)	476, 578, 1172, 1862
GlaI	GCGC	3 cut(s)	100, 160, 1260
GsaI	CCCAGC	1 cut(s)	594
GsuI	CTGGAG	1 cut(s)	612
HaeIII	GGCC	9 cut(s)	178, 246, 474, 763, 858, 874, 1566, 1965, 1983
HapII	CCGG	5 cut(s)	7, 141, 317, 859, 2434
HhaI	GCGC	3 cut(s)	101, 161, 1261
Hin6I	GCGC	3 cut(s)	99, 159, 1259
HinP1I	GCGC	3 cut(s)	99, 159, 1259
HincII	GTYRAC	1 cut(s)	1653
HindII	GTYRAC	1 cut(s)	1653
HindIII	AAGCTT	1 cut(s)	977
HinfI	GANTC	3 cut(s)	1007, 2178, 2239
HpaI	GTTAAC	1 cut(s)	1653
HpaII	CCGG	5 cut(s)	7, 141, 317, 859, 2434
HphI	GGTGA	3 cut(s)	1002, 1876, 2435
Hpy166II	GTNNAC	5 cut(s)	935, 1069, 1282, 1329, 1653
Hpy188III	TCNNGA	1 cut(s)	223
Hpy8I	GTNNAC	5 cut(s)	935, 1069, 1282, 1329, 1653
HpyAV	CCTTC	5 cut(s)	32, 197, 272, 538, 949
HpyCH4III	ACNGT	9 cut(s)	267, 487, 665, 1066, 1223, 1393, 1663, 2124, 2488
HpyCH4IV	ACGT	3 cut(s)	81, 805, 1230
HpyF3I	CTNAG	7 cut(s)	168, 567, 929, 1656, 1851, 2364, 2499
HpySE526I	ACGT	3 cut(s)	81, 805, 1230
HspAI	GCGC	3 cut(s)	99, 159, 1259
KflI	GGGWCCC	1 cut(s)	595
KpnI	GGTACC	1 cut(s)	2113
KroI	GCCGGC	1 cut(s)	140
KroNI	GCCGGC	1 cut(s)	142
KspAI	GTTAAC	1 cut(s)	1653
KspI	CCGCGG	1 cut(s)	51
Kzo9I	GATC	7 cut(s)	219, 409, 772, 958, 1584, 1870, 1927
LguI	GCTCTTC	2 cut(s)	420, 1350
LmnI	GCTCC	8 cut(s)	9, 15, 240, 631, 813, 910, 1408, 2020
Lsp1109I	GCAGC	5 cut(s)	397, 550, 955, 988, 1621
MabI	ACCWGGT	3 cut(s)	1782, 1886, 2105
MaeI	CTAG	4 cut(s)	476, 578, 1172, 1862
MaeII	ACGT	3 cut(s)	81, 805, 1230
MaeIII	GTNAC	4 cut(s)	1162, 1420, 2423, 2475
MalI	GATC	7 cut(s)	221, 411, 774, 960, 1586, 1872, 1929
MbiI	CCGCTC	1 cut(s)	243
MboI	GATC	7 cut(s)	219, 409, 772, 958, 1584, 1870, 1927
MfeI	CAATTG	1 cut(s)	909
MflI	RGATCY	4 cut(s)	409, 772, 958, 1927
MhlI	GDGCHC	2 cut(s)	2078, 2229
MlsI	TGGCCA	2 cut(s)	1566, 1983
MluNI	TGGCCA	2 cut(s)	1566, 1983
MlyI	GAGTC	1 cut(s)	2248
MmeI	TCCRAC	3 cut(s)	826, 2193, 2377
Mox20I	TGGCCA	2 cut(s)	1566, 1983
Mph1103I	ATGCAT	3 cut(s)	235, 649, 736
MroNI	GCCGGC	1 cut(s)	140
MroXI	GAANNNNTTC	3 cut(s)	311, 1385, 2348
MscI	TGGCCA	2 cut(s)	1566, 1983
MslI	CAYNNNNRTG	4 cut(s)	1053, 1416, 1771, 2125
Msp20I	TGGCCA	2 cut(s)	1566, 1983
MspA1I	CMGCKG	2 cut(s)	50, 388
MspI	CCGG	5 cut(s)	7, 141, 317, 859, 2434
MunI	CAATTG	1 cut(s)	909
Mva1269I	GAATGC	1 cut(s)	1124
MvaI	CCWGG	8 cut(s)	339, 630, 1342, 1784, 1888, 1967, 2107, 2375
MvnI	CGCG	4 cut(s)	50, 62, 99, 159
NaeI	GCCGGC	1 cut(s)	142
NciI	CCSGG	2 cut(s)	317, 2435
NcoI	CCATGG	2 cut(s)	599, 1196
NdeI	CATATG	1 cut(s)	529
NdeII	GATC	7 cut(s)	219, 409, 772, 958, 1584, 1870, 1927
NgoMIV	GCCGGC	1 cut(s)	140
NlaIV	GGNNCC	9 cut(s)	11, 450, 596, 597, 873, 1032, 1338, 2111, 2217
NmuCI	GTSAC	3 cut(s)	1420, 2423, 2475
NsiI	ATGCAT	3 cut(s)	235, 649, 736
NspI	RCATGY	6 cut(s)	130, 651, 968, 1052, 1537, 1724
NspV	TTCGAA	1 cut(s)	2342
PaeI	GCATGC	4 cut(s)	130, 651, 968, 1537
PceI	AGGCCT	1 cut(s)	763
PciI	ACATGT	2 cut(s)	1048, 1720
PciSI	GCTCTTC	2 cut(s)	420, 1350
PcsI	WCGNNNNNNNCGW	1 cut(s)	87
PctI	GAATGC	1 cut(s)	1124
PdiI	GCCGGC	1 cut(s)	142
PdmI	GAANNNNTTC	3 cut(s)	311, 1385, 2348
PfeI	GAWTC	2 cut(s)	1007, 2178
PflMI	CCANNNNNTGG	2 cut(s)	2024, 2489
PfoI	TCCNGGA	1 cut(s)	628
PleI	GAGTC	1 cut(s)	2247
PpsI	GAGTC	1 cut(s)	2247
PpuMI	RGGWCCY	1 cut(s)	595
PscI	ACATGT	2 cut(s)	1048, 1720
PsiI	TTATAA	1 cut(s)	2261
Psp1406I	AACGTT	1 cut(s)	1230
Psp5II	RGGWCCY	1 cut(s)	595
Psp6I	CCWGG	8 cut(s)	337, 628, 1340, 1782, 1886, 1965, 2105, 2373
PspEI	GGTNACC	1 cut(s)	2423
PspFI	CCCAGC	1 cut(s)	590
PspGI	CCWGG	8 cut(s)	337, 628, 1340, 1782, 1886, 1965, 2105, 2373
PspN4I	GGNNCC	9 cut(s)	11, 450, 596, 597, 873, 1032, 1338, 2111, 2217
PspPI	GGNCC	6 cut(s)	449, 595, 872, 1031, 1429, 1963
PspPPI	RGGWCCY	1 cut(s)	595
PstNI	CAGNNNCTG	1 cut(s)	1160
PsuI	RGATCY	4 cut(s)	409, 772, 958, 1927
RsaI	GTAC	5 cut(s)	269, 847, 1060, 1892, 2111
RsaNI	GTAC	5 cut(s)	268, 846, 1059, 1891, 2110
RseI	CAYNNNNRTG	4 cut(s)	1053, 1416, 1771, 2125
SacII	CCGCGG	1 cut(s)	51
SapI	GCTCTTC	2 cut(s)	420, 1350
Sau3AI	GATC	7 cut(s)	219, 409, 772, 958, 1584, 1870, 1927
Sau96I	GGNCC	6 cut(s)	449, 595, 872, 1031, 1429, 1963
ScaI	AGTACT	1 cut(s)	269
SchI	GAGTC	1 cut(s)	2248
SduI	GDGCHC	2 cut(s)	2078, 2229
SexAI	ACCWGGT	3 cut(s)	1782, 1886, 2105
SfcI	CTRYAG	2 cut(s)	464, 1222
Sfr303I	CCGCGG	1 cut(s)	51
SfuI	TTCGAA	1 cut(s)	2342
SgrBI	CCGCGG	1 cut(s)	51
SinI	GGWCC	4 cut(s)	449, 595, 1031, 1429
SmiI	ATTTAAAT	1 cut(s)	1416
SmiMI	CAYNNNNRTG	4 cut(s)	1053, 1416, 1771, 2125
SmlI	CTYRAG	4 cut(s)	1691, 2228, 2298, 2322
SmoI	CTYRAG	4 cut(s)	1691, 2228, 2298, 2322
SphI	GCATGC	4 cut(s)	130, 651, 968, 1537
SseBI	AGGCCT	1 cut(s)	763
SspI	AATATT	3 cut(s)	1404, 1464, 1507
SspMI	CTAG	4 cut(s)	476, 578, 1172, 1862
StuI	AGGCCT	1 cut(s)	763
StyI	CCWWGG	5 cut(s)	599, 1171, 1196, 1248, 1396
SwaI	ATTTAAAT	1 cut(s)	1416
TaaI	ACNGT	9 cut(s)	267, 487, 665, 1066, 1223, 1393, 1663, 2124, 2488
TaiI	ACGT	3 cut(s)	84, 808, 1233
TaqI	TCGA	4 cut(s)	111, 1527, 2050, 2342
TaqII	GACCGA	1 cut(s)	1577
TatI	WGTACW	1 cut(s)	267
TauI	GCSGC	6 cut(s)	50, 59, 62, 126, 246, 256
TfiI	GAWTC	2 cut(s)	1007, 2178
TscAI	CASTG	3 cut(s)	1121, 1249, 1585
TseFI	GTSAC	3 cut(s)	1420, 2423, 2475
TseI	GCWGC	5 cut(s)	385, 563, 968, 1001, 1609
Tsp45I	GTSAC	3 cut(s)	1420, 2423, 2475
TspDTI	ATGAA	3 cut(s)	519, 675, 930
TspGWI	ACGGA	2 cut(s)	1349, 2171
TspRI	CASTG	3 cut(s)	1121, 1249, 1585
Van91I	CCANNNNNTGG	2 cut(s)	2024, 2489
VpaK11BI	GGWCC	4 cut(s)	449, 595, 1031, 1429
XapI	RAATTY	6 cut(s)	347, 682, 1321, 1942, 2276, 2344
XceI	RCATGY	6 cut(s)	130, 651, 968, 1052, 1537, 1724
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1368
XmaJI	CCTAGG	1 cut(s)	1171
XmiI	GTMKAC	1 cut(s)	1328
XmnI	GAANNNNTTC	3 cut(s)	311, 1385, 2348
XspI	CTAG	4 cut(s)	476, 578, 1172, 1862
ZrmI	AGTACT	1 cut(s)	269
Zsp2I	ATGCAT	3 cut(s)	235, 649, 736

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.