Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0014328

Physical Location & Seq

Genomic Coordinates Forward (+)

32195099 .. 32206834

Gene Structure 11736 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG183700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2658 bp

ATGCTCCGGCTCCGATGCCTCCTTCTCTGCCTCACTATCCACTTCGCTGCGGTTGCCGCCGCGTTCTTCAAGCCCTTCAACGTCAGCTACGACCACCGCGCTCTCATCATCGATGGCAGCCGCCGCATGCTCATCTCCGCCGGCATCCACTACCCTCGCGCCACTCCCCAGATGTGGCCTGATTTGATTGCAAATAGCAAAGAAGGCGGGGCGGATGTGATCCAGACCTATGCATTTTGGAGCGGCCACGAGCCGCAGAGAGGACAGTACTATTTTGAAGGGAGATATGATATCGTCAAGTTTGTGAAGTTAGTGGGAGCTAGTGGACTCTATCTCCATCTTCGCATAGGGCCTTACGTCTGCGCTGAGTGGAACTTTGGTGGCTTCCCGGTGTGGTTGCGGGACATACCTGGAATAGAATTCCGAACAAACAATGCACCTTTTAAGGAAGAGATGCAGCGGTTTGTTAAAAAGATGGTGGATCTGATGCGAGAGGAAGAGCTATTTTCTTGGCAAGGTGGTCCAGTAATTATGCTACAGATTGAAAATGAATATGGAAATATGGAAAGCTCATATGGACAGAGAGGGAAGGAATATGTTAAGTGGGCTGCTACGATGGCTCTAGGTCTCGGTGCTGGGGTCCCATGGGTCATGTGCAAGCAAGTTGATGCTCCAGGAAGTGTTATAGATGCATGCAATGGATACTACTGTGATGGATACAAGTCAAATTCATACAACAAGCCAATTCTTTGGACAGAAGATTGGGATGGATGGTATGCATCATGGGGTGGAAGATTGCCTCACAGGCCTGTTGAAGATCTTGCATTTGCAGTAGCACGCTTTTTCCAACGTGGAGGGAGCTTTCAGAATTATTATATGTATTTTGGTGGTACAAACTTTGGCCGGACTTCTGGGGGGCCATTCTATATTACCAGTTATGATTATGATGCTCCAATTGATGAATATGGACTGCTGAGTGAACCTAAATGGGGACATCTGAAGGATCTACATGCTGCTATAAAGCTTTGTGAGCCTGCTCTTGTTGCTGCTGATTCACCGCATTACATTAAATTGGGACCAAAACAGGAGTTTGAGCCAAAACCTTTAGCACATGTATACAGTACAACAGTTCACACTAAAAGCTTAAACTTTACTTGGTTTGGTAGAAGTCAAAGCCAGTGCTCCGCATTCCTCGCAAACATTGATGAACATAAATCAGCCTCTGTAACATTCCTAGGGCAAAAATATAATCTGCCACCATGGTCAGTCAGTATATTGCCAGACTGTAGGAACGTTGTGTTCAACACTGCCAAGGTTGGGGCGCAAACTTCCATAAAAAGTGTAAACTCTGATTTGCCTCTTCCAGTTATTTCAGAGAACAAAAATTTGTATATCATGAAACCCTGGATGACTTTAGAAGAGCCAATTGGTATTTGGAGTGAGAACAATTTCACAGTCCGAGGAATATTGGAGCATTTAAATGTGACAAAGGACCATTCTGATTACCTGTGGCATATAACCAGAATATTTGTTTCAGATGAGGACATCTCTTTTTGGGAGGAAAGCAATATTAGTCCAGCAATTACAATCGATAGTATGCGAGATGTGTTGCGTGTATTTGTTAATGGCCAGCTTACAGGCAGTGTGATCGGTCATTGGGTCAAGGTAGTGCAGCCTGTTAGGTTTGTCAAGGGATATAATGACTTGTTGCTGTTAACTCAGACAGTTGGCTTGCAGAACTATGGTGCTTTCCTTGAGAAAGACGGGGCAGGATTTAGAGGACATGTCAAACTGACTGGGTTCAAAAATGGGGATATCGACCTCTCAACATTGTTATGGACGTACCAGGTTGGGCTTAAAGGAGAGTTTTTCAAAATATACACCATAGAAGAAAATGAGAAGGCAGGTTGGGCTGAGTTGTCGCTAGATGCCGATCCATCCACTTTTACCTGGTACAAGACATACTTTGATAGCCCTGCTGGCACAGATCCAGTTGCTCTAAATTTAGGAAGCATGGGGAAGGGCCAGGCTTGGGTCAATGGCCACCATATAGGAAGATATTGGACCCTTGTTGCTCCAAAAGATGGATGCCAAGAAATTTGTGATTATCGAGGGGCATACAACTCTGATAAGTGCTCAACAAACTGTGGGAAGCCTACTCAAACCTGGTACCATATTCCACGGTCATGGTTGCAGGCTTCAAGTAATTTACTTGTTATCTTTGAGGAAACTGGAGGGAATCCATTTGAGATTTCAATCAACTCGCGTGCAGTTGGAACAATCTGTGCTCAAGTGTCAGAGTCTCACTACCCACCTGTCCAGAAGTGGTTCAATCCGGATTTTATTGATGAAAAAGGCAGAGTAAATGATCGGACACCAGAAATTCAGTTGCAATGTCAAGACGGGTTTATAATATCTTCCATAGAATTTGCTAGCTATGGAAGTCCTCAAGGTCGTTGCCAGAAGTTTTCTCAAGGGAATTGCCATGCTTCAAATTCATCCTCCATAGTTTCTGAGGCTTGCCTGGGAAAGAATAGCTGTTCAGTAGGAGTATCAAATCTTGTATTTGGAGGTGACCCATGCCGGGGTATTGTTAAGACATTGGCTGTTGAGGCAAGATGCAGGTCGCTACCAACTGTTGGTTTTTCTCAGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0009505 GO:0015925 GO:0016020 GO:0016787 GO:0016798 GO:0030312 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

885

Amino Acids

99.37

Weight (kDa)

6.28

Isoelectric Point (pI)

38.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	35 - 339	3.9e-116	Glycosyl hydrolases family 35
GHD	PF17834	385 - 439	6e-25	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	650 - 736	1.5e-25	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	797 - 874	1.4e-22	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014328)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32810 AT2G32810
fragaria_vesca	FvH4_4g13490
malus_domestica	MD13G1283100.v1.1
prunus_persica	Prupe.1G160600_v2.0.a1 Prupe.1G160600_v2.0.a1
pyrus_communis	pycom420g01190
rosa_chinensis	RchiOBHm_Chr4g0414111
rosa_laevigata	RLG00000008175
rosa_multiflora	Rmu_sc0003264.1_g000008
rosa_roxburghii	Rroxscaffold_5G00358040
rosa_rugosa	Rorug04G0125600 Rorug04G0125700
rosa_samantha	Rh4AG184200 Rh4BG183700 Rh4DG179600
rosa_wichuraiana	Rw4G015680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2411
Acc36I	ACCTGC	2 cut(s)	1895, 2613
Acc65I	GGTACC	1 cut(s)	2169
AccB1I	GGYRCC	1 cut(s)	2169
AccB7I	CCANNNNNTGG	2 cut(s)	2084, 2639
AccBSI	CCGCTC	1 cut(s)	243
AccI	GTMKAC	1 cut(s)	1116
AccII	CGCG	4 cut(s)	62, 99, 159, 2266
AccIII	TCCGGA	1 cut(s)	2335
AclI	AACGTT	1 cut(s)	1293
AclWI	GGATC	5 cut(s)	214, 489, 1011, 1928, 1982
AcoI	YGGCCR	4 cut(s)	244, 901, 1627, 2041
AcsI	RAATTY	8 cut(s)	419, 727, 1384, 2002, 2097, 2382, 2426, 2494
AcuI	CTGAAG	1 cut(s)	1019
AfaI	GTAC	6 cut(s)	269, 892, 1123, 1844, 1955, 2171
AfiI	CCNNNNNNNGG	9 cut(s)	174, 260, 989, 1317, 2032, 2084, 2584, 2585, 2639
AflIII	ACRYGT	2 cut(s)	1111, 1783
AjnI	CCWGG	8 cut(s)	409, 673, 1403, 1845, 1949, 2025, 2165, 2523
AjuI	GAANNNNNNNTTGG	2 cut(s)	1410, 1442
Alw21I	GWGCWC	3 cut(s)	1184, 2138, 2290
Alw26I	GTCTC	2 cut(s)	632, 2307
AlwI	GGATC	5 cut(s)	214, 489, 1011, 1928, 1982
AlwNI	CAGNNNCTG	1 cut(s)	1223
Aor13HI	TCCGGA	1 cut(s)	2335
AoxI	GGCC	9 cut(s)	176, 244, 350, 806, 901, 917, 1627, 2023, 2041
ApeKI	GCWGC	7 cut(s)	47, 117, 457, 608, 1013, 1046, 1672
ApoI	RAATTY	8 cut(s)	419, 727, 1384, 2002, 2097, 2382, 2426, 2494
Asp700I	GAANNNNTTC	2 cut(s)	1448, 2327
Asp718I	GGTACC	1 cut(s)	2169
AspA2I	CCTAGG	1 cut(s)	1234
AspLEI	GCGC	4 cut(s)	101, 161, 365, 1324
AspS9I	GGNCC	8 cut(s)	350, 521, 640, 917, 1076, 1492, 2023, 2064
AsuC2I	CCSGG	2 cut(s)	389, 2585
AsuHPI	GGTGA	2 cut(s)	1047, 2585
AsuNHI	GCTAGC	1 cut(s)	2432
AvaII	GGWCC	5 cut(s)	521, 640, 1076, 1492, 2064
AvrII	CCTAGG	1 cut(s)	1234
BaeI	ACNNNNGTAYC	2 cut(s)	694, 727
BalI	TGGCCA	2 cut(s)	1629, 2043
BanI	GGYRCC	1 cut(s)	2169
BauI	CACGAG	1 cut(s)	248
Bbv12I	GWGCWC	3 cut(s)	1184, 2138, 2290
BbvI	GCAGC	7 cut(s)	34, 129, 469, 595, 1000, 1033, 1684
BccI	CCATC	9 cut(s)	107, 345, 469, 610, 707, 761, 765, 1945, 2078
BcgI	CGANNNNNNTGC	2 cut(s)	1630, 1664
BciT130I	CCWGG	8 cut(s)	411, 675, 1405, 1847, 1951, 2027, 2167, 2525
BciVI	GTATCC	2 cut(s)	695, 710
BcnI	CCSGG	2 cut(s)	389, 2585
BcoDI	GTCTC	2 cut(s)	632, 2307
BfaI	CTAG	5 cut(s)	321, 623, 1235, 1925, 2433
BfmI	CTRYAG	2 cut(s)	536, 1285
BfuAI	ACCTGC	2 cut(s)	1895, 2613
BfuI	GTATCC	2 cut(s)	695, 710
BglI	GCCNNNNNGGC	2 cut(s)	805, 1980
BglII	AGATCT	1 cut(s)	817
BlnI	CCTAGG	1 cut(s)	1234
BmcAI	AGTACT	1 cut(s)	269
Bme18I	GGWCC	5 cut(s)	521, 640, 1076, 1492, 2064
BmgT120I	GGNCC	8 cut(s)	350, 521, 640, 917, 1076, 1492, 2023, 2064
BmiI	GGNNCC	7 cut(s)	11, 641, 642, 918, 1077, 2066, 2171
BmrI	ACTGGG	1 cut(s)	1806
BmtI	GCTAGC	1 cut(s)	2436
BmuI	ACTGGG	1 cut(s)	1806
BplI	GAGNNNNNCTC	2 cut(s)	1532, 1564
BpmI	CTGGAG	2 cut(s)	657, 2253
BpuEI	CTTGAG	4 cut(s)	1775, 2274, 2433, 2457
BpuMI	CCSGG	2 cut(s)	389, 2585
Bsa29I	ATCGAT	2 cut(s)	111, 1590
BsaBI	GATNNNNATC	2 cut(s)	1932, 2255
BsaI	GGTCTC	1 cut(s)	632
BsaJI	CCNNGG	9 cut(s)	644, 1234, 1259, 1311, 1403, 1459, 2180, 2524, 2584
BsaWI	WCCGGW	1 cut(s)	2335
BsaXI	ACNNNNNCTCC	2 cut(s)	318, 348
Bsc4I	CCNNNNNNNGG	9 cut(s)	174, 260, 989, 1317, 2032, 2084, 2584, 2585, 2639
Bse118I	RCCGGY	1 cut(s)	140
Bse1I	ACTGG	7 cut(s)	524, 933, 1177, 1364, 1801, 1991, 2236
Bse3DI	GCAATG	2 cut(s)	703, 2399
Bse8I	GATNNNNATC	2 cut(s)	1932, 2255
BseAI	TCCGGA	1 cut(s)	2335
BseBI	CCWGG	8 cut(s)	411, 675, 1405, 1847, 1951, 2027, 2167, 2525
BseCI	ATCGAT	2 cut(s)	111, 1590
BseDI	CCNNGG	9 cut(s)	644, 1234, 1259, 1311, 1403, 1459, 2180, 2524, 2584
BseGI	GGATG	8 cut(s)	144, 220, 772, 776, 1413, 1937, 2093, 2498
BseJI	GATNNNNATC	2 cut(s)	1932, 2255
BseLI	CCNNNNNNNGG	9 cut(s)	174, 260, 989, 1317, 2032, 2084, 2584, 2585, 2639
BseMI	GCAATG	2 cut(s)	703, 2399
BseMII	CTCAG	5 cut(s)	357, 965, 1733, 1905, 2505
BseNI	ACTGG	7 cut(s)	524, 933, 1177, 1364, 1801, 1991, 2236
BseXI	GCAGC	7 cut(s)	34, 129, 469, 595, 1000, 1033, 1684
BseYI	CCCAGC	1 cut(s)	635
BsgI	GTGCAG	2 cut(s)	1691, 2289
Bsh1236I	CGCG	4 cut(s)	62, 99, 159, 2266
BshFI	GGCC	9 cut(s)	178, 246, 352, 808, 903, 919, 1629, 2025, 2043
BshNI	GGYRCC	1 cut(s)	2169
BshVI	ATCGAT	2 cut(s)	111, 1590
BsiHKAI	GWGCWC	3 cut(s)	1184, 2138, 2290
BsiSI	CCGG	6 cut(s)	7, 141, 389, 904, 2336, 2584
BslFI	GGGAC	4 cut(s)	416, 626, 1005, 1089
BslI	CCNNNNNNNGG	9 cut(s)	174, 260, 989, 1317, 2032, 2084, 2584, 2585, 2639
BsmAI	GTCTC	2 cut(s)	632, 2307
BsmFI	GGGAC	4 cut(s)	416, 626, 1005, 1089
BsmI	GAATGC	1 cut(s)	1187
BsnI	GGCC	9 cut(s)	178, 246, 352, 808, 903, 919, 1629, 2025, 2043
Bso31I	GGTCTC	1 cut(s)	632
Bsp1286I	GDGCHC	3 cut(s)	1184, 2138, 2290
Bsp13I	TCCGGA	1 cut(s)	2335
Bsp143I	GATC	8 cut(s)	219, 481, 817, 1003, 1647, 1933, 1987, 2368
Bsp19I	CCATGG	2 cut(s)	644, 1259
BspANI	GGCC	9 cut(s)	178, 246, 352, 808, 903, 919, 1629, 2025, 2043
BspCNI	CTCAG	5 cut(s)	358, 966, 1732, 1906, 2506
BspDI	ATCGAT	2 cut(s)	111, 1590
BspEI	TCCGGA	1 cut(s)	2335
BspFNI	CGCG	4 cut(s)	62, 99, 159, 2266
BspHI	TCATGA	1 cut(s)	1395
BspLI	GGNNCC	7 cut(s)	11, 641, 642, 918, 1077, 2066, 2171
BspMI	ACCTGC	2 cut(s)	1895, 2613
BspOI	GCTAGC	1 cut(s)	2436
BspPI	GGATC	5 cut(s)	214, 489, 1011, 1928, 1982
BspQI	GCTCTTC	2 cut(s)	492, 1413
BspT107I	GGYRCC	1 cut(s)	2169
BspTNI	GGTCTC	1 cut(s)	632
BsrBI	CCGCTC	1 cut(s)	243
BsrDI	GCAATG	2 cut(s)	703, 2399
BsrFI	RCCGGY	1 cut(s)	140
BsrI	ACTGG	7 cut(s)	524, 933, 1177, 1364, 1801, 1991, 2236
BssAI	RCCGGY	1 cut(s)	140
BssECI	CCNNGG	9 cut(s)	644, 1234, 1259, 1311, 1403, 1459, 2180, 2524, 2584
BssMI	GATC	8 cut(s)	219, 481, 817, 1003, 1647, 1933, 1987, 2368
BssNAI	GTATAC	1 cut(s)	1117
BssSI	CACGAG	1 cut(s)	248
BssT1I	CCWWGG	4 cut(s)	644, 1234, 1259, 1311
Bst1107I	GTATAC	1 cut(s)	1117
Bst2BI	CACGAG	1 cut(s)	248
Bst2UI	CCWGG	8 cut(s)	411, 675, 1405, 1847, 1951, 2027, 2167, 2525
Bst6I	CTCTTC	4 cut(s)	444, 492, 1365, 1413
BstDEI	CTNAG	6 cut(s)	366, 974, 1719, 1914, 2514, 2649
BstDSI	CCRYGG	3 cut(s)	644, 1259, 2180
BstEII	GGTNACC	1 cut(s)	2573
BstF5I	GGATG	8 cut(s)	144, 220, 772, 776, 1413, 1937, 2093, 2498
BstFNI	CGCG	4 cut(s)	62, 99, 159, 2266
BstHHI	GCGC	4 cut(s)	101, 161, 365, 1324
BstKTI	GATC	8 cut(s)	222, 484, 820, 1006, 1650, 1936, 1990, 2371
BstMAI	GTCTC	2 cut(s)	632, 2307
BstMBI	GATC	8 cut(s)	219, 481, 817, 1003, 1647, 1933, 1987, 2368
BstNI	CCWGG	8 cut(s)	411, 675, 1405, 1847, 1951, 2027, 2167, 2525
BstNSI	RCATGY	5 cut(s)	130, 696, 1013, 1115, 1787
BstPI	GGTNACC	1 cut(s)	2573
BstSFI	CTRYAG	2 cut(s)	536, 1285
BstUI	CGCG	4 cut(s)	62, 99, 159, 2266
BstV1I	GCAGC	7 cut(s)	34, 129, 469, 595, 1000, 1033, 1684
BstX2I	RGATCY	4 cut(s)	481, 817, 1003, 1987
BstXI	CCANNNNNNTGG	2 cut(s)	750, 2187
BstYI	RGATCY	4 cut(s)	481, 817, 1003, 1987
BstZ17I	GTATAC	1 cut(s)	1117
Bsu15I	ATCGAT	2 cut(s)	111, 1590
BsuI	GTATCC	2 cut(s)	695, 710
BsuRI	GGCC	9 cut(s)	178, 246, 352, 808, 903, 919, 1629, 2025, 2043
BsuTUI	ATCGAT	2 cut(s)	111, 1590
BtgI	CCRYGG	3 cut(s)	644, 1259, 2180
BtsCI	GGATG	8 cut(s)	144, 220, 772, 776, 1413, 1937, 2093, 2498
BtsI	GCAGTG	2 cut(s)	1305, 1648
BtsIMutI	CAGTG	3 cut(s)	1184, 1305, 1648
BveI	ACCTGC	2 cut(s)	1895, 2613
CaiI	CAGNNNCTG	1 cut(s)	1223
CciI	TCATGA	1 cut(s)	1395
CfoI	GCGC	4 cut(s)	101, 161, 365, 1324
Cfr10I	RCCGGY	1 cut(s)	140
Cfr13I	GGNCC	8 cut(s)	350, 521, 640, 917, 1076, 1492, 2023, 2064
ClaI	ATCGAT	2 cut(s)	111, 1590
CsiI	ACCWGGT	3 cut(s)	1845, 1949, 2165
Csp6I	GTAC	6 cut(s)	268, 891, 1122, 1843, 1954, 2170
CviQI	GTAC	6 cut(s)	268, 891, 1122, 1843, 1954, 2170
DdeI	CTNAG	6 cut(s)	366, 974, 1719, 1914, 2514, 2649
DpnI	GATC	8 cut(s)	221, 483, 819, 1005, 1649, 1935, 1989, 2370
DpnII	GATC	8 cut(s)	219, 481, 817, 1003, 1647, 1933, 1987, 2368
DraI	TTTAAA	1 cut(s)	1479
EaeI	YGGCCR	4 cut(s)	244, 901, 1627, 2041
Eam1104I	CTCTTC	4 cut(s)	444, 492, 1365, 1413
EarI	CTCTTC	4 cut(s)	444, 492, 1365, 1413
EciI	GGCGGA	2 cut(s)	127, 227
Eco130I	CCWWGG	4 cut(s)	644, 1234, 1259, 1311
Eco147I	AGGCCT	1 cut(s)	808
Eco31I	GGTCTC	1 cut(s)	632
Eco32I	GATATC	2 cut(s)	292, 1816
Eco47I	GGWCC	5 cut(s)	521, 640, 1076, 1492, 2064
Eco57I	CTGAAG	1 cut(s)	1019
Eco91I	GGTNACC	1 cut(s)	2573
EcoO109I	RGGNCCY	2 cut(s)	350, 640
EcoO65I	GGTNACC	1 cut(s)	2573
EcoRI	GAATTC	1 cut(s)	419
EcoRII	CCWGG	8 cut(s)	409, 673, 1403, 1845, 1949, 2025, 2165, 2523
EcoRV	GATATC	2 cut(s)	292, 1816
EcoT14I	CCWWGG	4 cut(s)	644, 1234, 1259, 1311
EcoT22I	ATGCAT	3 cut(s)	235, 694, 781
ErhI	CCWWGG	4 cut(s)	644, 1234, 1259, 1311
FalI	AAGNNNNNCTT	2 cut(s)	1949, 1981
FaqI	GGGAC	4 cut(s)	416, 626, 1005, 1089
FauI	CCCGC	2 cut(s)	200, 393
FauNDI	CATATG	1 cut(s)	574
FblI	GTMKAC	1 cut(s)	1116
FokI	GGATG	8 cut(s)	131, 227, 779, 783, 1420, 1924, 2100, 2485
FspBI	CTAG	5 cut(s)	321, 623, 1235, 1925, 2433
GlaI	GCGC	4 cut(s)	100, 160, 364, 1323
GsaI	CCCAGC	1 cut(s)	639
GsuI	CTGGAG	2 cut(s)	657, 2253
HaeIII	GGCC	9 cut(s)	178, 246, 352, 808, 903, 919, 1629, 2025, 2043
HapII	CCGG	6 cut(s)	7, 141, 389, 904, 2336, 2584
HhaI	GCGC	4 cut(s)	101, 161, 365, 1324
Hin6I	GCGC	4 cut(s)	99, 159, 363, 1322
HinP1I	GCGC	4 cut(s)	99, 159, 363, 1322
HincII	GTYRAC	1 cut(s)	1716
HindII	GTYRAC	1 cut(s)	1716
HindIII	AAGCTT	2 cut(s)	1022, 1141
HinfI	GANTC	4 cut(s)	327, 1052, 2239, 2300
HpaI	GTTAAC	1 cut(s)	1716
HpaII	CCGG	6 cut(s)	7, 141, 389, 904, 2336, 2584
HphI	GGTGA	2 cut(s)	1047, 2585
Hpy166II	GTNNAC	6 cut(s)	326, 980, 1117, 1132, 1345, 1716
Hpy188III	TCNNGA	5 cut(s)	223, 1396, 2320, 2336, 2399
Hpy8I	GTNNAC	6 cut(s)	326, 980, 1117, 1132, 1345, 1716
HpyAV	CCTTC	8 cut(s)	32, 85, 197, 272, 583, 994, 1894, 2014
HpyCH4IV	ACGT	5 cut(s)	81, 357, 850, 1293, 1841
HpyF3I	CTNAG	6 cut(s)	366, 974, 1719, 1914, 2514, 2649
HpySE526I	ACGT	5 cut(s)	81, 357, 850, 1293, 1841
HspAI	GCGC	4 cut(s)	99, 159, 363, 1322
KflI	GGGWCCC	1 cut(s)	640
Kpn2I	TCCGGA	1 cut(s)	2335
KpnI	GGTACC	1 cut(s)	2173
KroI	GCCGGC	1 cut(s)	140
KroNI	GCCGGC	1 cut(s)	142
KspAI	GTTAAC	1 cut(s)	1716
Kzo9I	GATC	8 cut(s)	219, 481, 817, 1003, 1647, 1933, 1987, 2368
LguI	GCTCTTC	2 cut(s)	492, 1413
Lsp1109I	GCAGC	7 cut(s)	34, 129, 469, 595, 1000, 1033, 1684
MabI	ACCWGGT	3 cut(s)	1845, 1949, 2165
MaeI	CTAG	5 cut(s)	321, 623, 1235, 1925, 2433
MaeII	ACGT	5 cut(s)	81, 357, 850, 1293, 1841
MaeIII	GTNAC	3 cut(s)	1225, 1483, 2573
MalI	GATC	8 cut(s)	221, 483, 819, 1005, 1649, 1935, 1989, 2370
MbiI	CCGCTC	1 cut(s)	243
MboI	GATC	8 cut(s)	219, 481, 817, 1003, 1647, 1933, 1987, 2368
MfeI	CAATTG	2 cut(s)	954, 1425
MflI	RGATCY	4 cut(s)	481, 817, 1003, 1987
MhlI	GDGCHC	3 cut(s)	1184, 2138, 2290
MlsI	TGGCCA	2 cut(s)	1629, 2043
MluNI	TGGCCA	2 cut(s)	1629, 2043
MlyI	GAGTC	2 cut(s)	321, 2309
MmeI	TCCRAC	2 cut(s)	871, 2254
Mox20I	TGGCCA	2 cut(s)	1629, 2043
Mph1103I	ATGCAT	3 cut(s)	235, 694, 781
MroI	TCCGGA	1 cut(s)	2335
MroNI	GCCGGC	1 cut(s)	140
MroXI	GAANNNNTTC	2 cut(s)	1448, 2327
MscI	TGGCCA	2 cut(s)	1629, 2043
MslI	CAYNNNNRTG	3 cut(s)	1479, 1834, 2185
Msp20I	TGGCCA	2 cut(s)	1629, 2043
MspA1I	CMGCKG	1 cut(s)	460
MspI	CCGG	6 cut(s)	7, 141, 389, 904, 2336, 2584
MunI	CAATTG	2 cut(s)	954, 1425
Mva1269I	GAATGC	1 cut(s)	1187
MvaI	CCWGG	8 cut(s)	411, 675, 1405, 1847, 1951, 2027, 2167, 2525
MvnI	CGCG	4 cut(s)	62, 99, 159, 2266
NaeI	GCCGGC	1 cut(s)	142
NciI	CCSGG	2 cut(s)	389, 2585
NcoI	CCATGG	2 cut(s)	644, 1259
NdeI	CATATG	1 cut(s)	574
NdeII	GATC	8 cut(s)	219, 481, 817, 1003, 1647, 1933, 1987, 2368
NgoMIV	GCCGGC	1 cut(s)	140
NheI	GCTAGC	1 cut(s)	2432
NlaIV	GGNNCC	7 cut(s)	11, 641, 642, 918, 1077, 2066, 2171
NmuCI	GTSAC	2 cut(s)	1483, 2573
NsiI	ATGCAT	3 cut(s)	235, 694, 781
NspI	RCATGY	5 cut(s)	130, 696, 1013, 1115, 1787
PaeI	GCATGC	2 cut(s)	130, 696
PagI	TCATGA	1 cut(s)	1395
PceI	AGGCCT	1 cut(s)	808
PciI	ACATGT	2 cut(s)	1111, 1783
PciSI	GCTCTTC	2 cut(s)	492, 1413
PcsI	WCGNNNNNNNCGW	1 cut(s)	87
PctI	GAATGC	1 cut(s)	1187
PdiI	GCCGGC	1 cut(s)	142
PdmI	GAANNNNTTC	2 cut(s)	1448, 2327
PfeI	GAWTC	2 cut(s)	1052, 2239
PflMI	CCANNNNNTGG	2 cut(s)	2084, 2639
PfoI	TCCNGGA	1 cut(s)	673
PleI	GAGTC	2 cut(s)	321, 2308
PpsI	GAGTC	2 cut(s)	321, 2308
PpuMI	RGGWCCY	1 cut(s)	640
PscI	ACATGT	2 cut(s)	1111, 1783
PsiI	TTATAA	1 cut(s)	2411
Psp1406I	AACGTT	1 cut(s)	1293
Psp5II	RGGWCCY	1 cut(s)	640
Psp6I	CCWGG	8 cut(s)	409, 673, 1403, 1845, 1949, 2025, 2165, 2523
PspEI	GGTNACC	1 cut(s)	2573
PspFI	CCCAGC	1 cut(s)	635
PspGI	CCWGG	8 cut(s)	409, 673, 1403, 1845, 1949, 2025, 2165, 2523
PspN4I	GGNNCC	7 cut(s)	11, 641, 642, 918, 1077, 2066, 2171
PspPI	GGNCC	8 cut(s)	350, 521, 640, 917, 1076, 1492, 2023, 2064
PspPPI	RGGWCCY	1 cut(s)	640
PstNI	CAGNNNCTG	1 cut(s)	1223
PsuI	RGATCY	4 cut(s)	481, 817, 1003, 1987
RsaI	GTAC	6 cut(s)	269, 892, 1123, 1844, 1955, 2171
RsaNI	GTAC	6 cut(s)	268, 891, 1122, 1843, 1954, 2170
RseI	CAYNNNNRTG	3 cut(s)	1479, 1834, 2185
SapI	GCTCTTC	2 cut(s)	492, 1413
Sau3AI	GATC	8 cut(s)	219, 481, 817, 1003, 1647, 1933, 1987, 2368
Sau96I	GGNCC	8 cut(s)	350, 521, 640, 917, 1076, 1492, 2023, 2064
ScaI	AGTACT	1 cut(s)	269
SchI	GAGTC	2 cut(s)	321, 2309
SduI	GDGCHC	3 cut(s)	1184, 2138, 2290
SexAI	ACCWGGT	3 cut(s)	1845, 1949, 2165
SfcI	CTRYAG	2 cut(s)	536, 1285
SinI	GGWCC	5 cut(s)	521, 640, 1076, 1492, 2064
SmiI	ATTTAAAT	1 cut(s)	1479
SmiMI	CAYNNNNRTG	3 cut(s)	1479, 1834, 2185
SmlI	CTYRAG	4 cut(s)	1754, 2289, 2448, 2472
SmoI	CTYRAG	4 cut(s)	1754, 2289, 2448, 2472
SphI	GCATGC	2 cut(s)	130, 696
SseBI	AGGCCT	1 cut(s)	808
SspI	AATATT	3 cut(s)	1467, 1527, 1570
SspMI	CTAG	5 cut(s)	321, 623, 1235, 1925, 2433
StuI	AGGCCT	1 cut(s)	808
StyI	CCWWGG	4 cut(s)	644, 1234, 1259, 1311
SwaI	ATTTAAAT	1 cut(s)	1479
TaiI	ACGT	5 cut(s)	84, 360, 853, 1296, 1844
TaqI	TCGA	4 cut(s)	111, 1590, 1818, 2110
TaqII	GACCGA	1 cut(s)	1640
TatI	WGTACW	2 cut(s)	267, 1121
TauI	GCSGC	6 cut(s)	59, 62, 123, 126, 246, 256
TfiI	GAWTC	2 cut(s)	1052, 2239
TscAI	CASTG	3 cut(s)	1184, 1312, 1648
TseFI	GTSAC	2 cut(s)	1483, 2573
TseI	GCWGC	7 cut(s)	47, 117, 457, 608, 1013, 1046, 1672
Tsp45I	GTSAC	2 cut(s)	1483, 2573
TspDTI	ATGAA	7 cut(s)	564, 720, 975, 1221, 1412, 2364, 2487
TspRI	CASTG	3 cut(s)	1184, 1312, 1648
Van91I	CCANNNNNTGG	2 cut(s)	2084, 2639
VpaK11BI	GGWCC	5 cut(s)	521, 640, 1076, 1492, 2064
XapI	RAATTY	8 cut(s)	419, 727, 1384, 2002, 2097, 2382, 2426, 2494
XceI	RCATGY	5 cut(s)	130, 696, 1013, 1115, 1787
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1431
XmaJI	CCTAGG	1 cut(s)	1234
XmiI	GTMKAC	1 cut(s)	1116
XmnI	GAANNNNTTC	2 cut(s)	1448, 2327
XspI	CTAG	5 cut(s)	321, 623, 1235, 1925, 2433
ZrmI	AGTACT	1 cut(s)	269
Zsp2I	ATGCAT	3 cut(s)	235, 694, 781

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.