Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0014328

Physical Location & Seq

Genomic Coordinates Forward (+)

45777097 .. 45788820

Gene Structure 11724 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG184200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2640 bp

ATGCTCCGGCTCCGATGCCTCCTTCTCTGCCTCACTATCCACTTCGCCGCGGTTGCCGCTGCGTTCTTCAAGCCGTTCAACGTCAGCTATGACCACCGCGCTCTCATCATCGATGGCAACCGCCGCATGCTCATCTCCGCCGGCATCCACTACCCTCGCGCCACTCCCCAGATGTGGCCTGATTTGATTGCAAAGAGCAAGGAAGGCGGGGCGGATGTGATCCAGACCTATGCATTTTGGAGCGGCCACGAGCCGCAGAGAGGACAGTACTATTTTGAAGGGAGATATGATATCGTCAAGTTTGTGAAGTTAGTGGGAGCTAGTGGACTCTATCTCCATCTTCGCATAGGGCCTTACGTCTGCGCTGAGTGGAACTTTGGTGGCTTCCCGGTGTGGTTGCGGGACATACCTGGAATAGAATTCCGAACAAACAATGCACCTTTTAAGGAAGAGATGCAGCGGTTTGTTAAAAAGATGGTGGATCTGATGCAAGAGGAAGAGCTGTTTTGTTGGCAAGGTGGTCCAGTAATTATGCTACAGATTGAAAATGAATATGGAAATATGGAAAGCTCATATGGACAGAGAGGGAAGGAATATGTTAAGTGGGCTGCTGAGATGGCTCTAGGTCTCGGTGCTGGGGTCCCATGGGTCATGTGCAAGCAAGTTGATGCTCCAGGAAGTATTATAGATGCATGCAATGGATACTACTGTGATGGATACAAGTCAAATTCATATAATAAGCCAATTCTTTGGACAGAAGATTGGGATGGATGGTATGCATCATGGGGTGGAAGATTGCCTCACAGGCCTGTTGAAGATCTTGCATTTGCAGTAGCACGCTTTTTCCAACGTGGAGGGAGCTTTCAGAATTATTATATGTATTTTGGTGGTACGAACTTTGGCCGGACTTCTGGGGGGCCATTTTATATTACCAGTTATGATTATGATGCTCCAATTGATGAATATGGACTGCTGAGTGAACCTAAATGGGGACATCTGAAGGATCTACATGCTGCTATAAAGCTTTGTGAGCCTGCTCTTGTTGCTGCTGATTCACCGGATTACATTAAATTGGGACCAAAACAGGAGGCACATGTATACGGTTCAACAGTTCACACTAAAGGCTTAAACTTTACTTGGTTTGGTAGAAGTCAAAGCCAGTGTTCCGCATTCCTCGCAAACATTGATGAACATAGATCAGCTTCTGTAACATTCCTAGGTCAAACATATAATCTGCCACCATGGTCAGTCAGTATATTGCCAGACTGTAGGAACGTTGTGTTCAACACTGCCAAGGTTGGGGCGCAAACTTCCATAAAAAGAGTAAACTCTGATTTGCCTCTTCCAGTTATTTCCGAGAACAAAAATTTGTATACCATGAAACCCTGGATGACTTTAGAAGAGCCAATTGGTATTTGGAGTGAGAATAATTTTACAGTCCTAGGAATATTGGAGCATTTAAATGTGACAAAGGACCATTCTGATTACCTGTGGCATATAACCAGAATATTTGTTTCAGATGAGGACATCTCTTTTTGGGAGGAAAGCAATATTAGTCCAGCAATTACAATCGATAGCATGCGAGATGTGTTGCGTGTATTTGTTAATGGCCAGCTTACAGGCAGTGTGATCGGTCATTGGGTCAAGGTAGTGCAGCCTGTTAGGTTTGTCAAGGGATATAATGACTTGTTGCTGTTAACTCAGACAGTTGGCTTGCAGAACTATGGTGCTTTCCTTGAGAAAGATGGGGCAGGATTTAGAGGACATGTCAAACTGACTGGGTTTAAAAATGGGGATATCGACCTCTCAACATTGTTATGGACTTACCAGGTTGGGCTTAAAGGAGAGTTTTTCAAAATATACACCATAGAAGAAAATGAGAAGGCAGGTTGGGCTGAGTTGTCACTGGAAGCTGATCCATCCACTTTTACCTGGTACAAGACATACTTTGATAGCCCTGCTGGCACAGATCCAGTTGCTCTAAATTTAGGAAGCATGGGAAAGGGCCAGGCTTGGGTCAATGGCCACCATATAGGAAGGTATTGGACACTTGTTGCTCCAAAAGATGGATGCCAAGAAATCTGTGATTATCGAGGGGCATACAACTCCGATAAGTGCTCAACAAATTGTGGGAAGCCTACTCAAACCTGGTACCATATTCCACGGTCATGGTTGCAGGCTTCAAGTAATTTACTTGTTATCTTTGAGGAAATTGGAGGGAATCCATTTGAGATTTCAATCAACTCGCGTGCAGTTGGAACCATCTGTGCTCAAGTGTCAGAATCTCACTACCCACCTGTCCAGAAGTGGTTCAGTCCAGATTTTATTGATGAAAAAGGCAGAGTAAATGATCGGACACCAGAAATTCACTTGCAATGTCAAGACGGGTTTATAATATCTTCCATAGAATTTGCTAGCTATGGAAGTCCTCAAGGTCGTTGCCAGAAGTTTTCTCAAGGGAATTGCCATGCTTTGAATTCTTTCTCCATAGTTTCCGAGGCTTGCCTGGGAAAGAATAGCTGTTCAGTCGGAGTATCAAATCTTGTATTTGGAGATGACCCATGCCGGGGTATTGTTAAGACGTTGGCTGTTGAGGCAAGATGCAGGTCACTACCAACCGTTGGTTTTTCTCAGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0009505 GO:0015925 GO:0016020 GO:0016787 GO:0016798 GO:0030312 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

879

Amino Acids

98.81

Weight (kDa)

6.02

Isoelectric Point (pI)

38.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	35 - 339	5.4e-117	Glycosyl hydrolases family 35
GHD	PF17834	379 - 433	1.3e-24	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	644 - 730	1.5e-25	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	791 - 868	3.1e-22	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014328)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32810 AT2G32810
fragaria_vesca	FvH4_4g13490
malus_domestica	MD13G1283100.v1.1
prunus_persica	Prupe.1G160600_v2.0.a1 Prupe.1G160600_v2.0.a1
pyrus_communis	pycom420g01190
rosa_chinensis	RchiOBHm_Chr4g0414111
rosa_laevigata	RLG00000008175
rosa_multiflora	Rmu_sc0003264.1_g000008
rosa_roxburghii	Rroxscaffold_5G00358040
rosa_rugosa	Rorug04G0125600 Rorug04G0125700
rosa_samantha	Rh4AG184200 Rh4BG183700 Rh4DG179600
rosa_wichuraiana	Rw4G015680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2393
Acc36I	ACCTGC	2 cut(s)	1877, 2595
Acc65I	GGTACC	1 cut(s)	2151
AccB1I	GGYRCC	1 cut(s)	2151
AccB7I	CCANNNNNTGG	2 cut(s)	2066, 2621
AccBSI	CCGCTC	1 cut(s)	243
AccI	GTMKAC	2 cut(s)	1098, 1373
AccII	CGCG	4 cut(s)	50, 99, 159, 2248
AclI	AACGTT	1 cut(s)	1275
AclWI	GGATC	5 cut(s)	214, 489, 1011, 1910, 1964
AcoI	YGGCCR	4 cut(s)	244, 901, 1609, 2023
AcsI	RAATTY	7 cut(s)	419, 727, 1366, 1984, 2364, 2408, 2476
AcuI	CTGAAG	1 cut(s)	1019
AfaI	GTAC	4 cut(s)	269, 892, 1937, 2153
AfiI	CCNNNNNNNGG	9 cut(s)	174, 260, 989, 1299, 2014, 2066, 2566, 2567, 2621
AflIII	ACRYGT	2 cut(s)	1093, 1765
AjnI	CCWGG	8 cut(s)	409, 673, 1385, 1827, 1931, 2007, 2147, 2505
AjuI	GAANNNNNNNTTGG	2 cut(s)	1392, 1424
Alw21I	GWGCWC	2 cut(s)	2120, 2272
Alw26I	GTCTC	1 cut(s)	632
AlwI	GGATC	5 cut(s)	214, 489, 1011, 1910, 1964
AlwNI	CAGNNNCTG	1 cut(s)	1205
AoxI	GGCC	9 cut(s)	176, 244, 350, 806, 901, 917, 1609, 2005, 2023
ApeKI	GCWGC	6 cut(s)	59, 457, 608, 1013, 1046, 1654
ApoI	RAATTY	7 cut(s)	419, 727, 1366, 1984, 2364, 2408, 2476
Asp700I	GAANNNNTTC	2 cut(s)	2309, 2480
Asp718I	GGTACC	1 cut(s)	2151
AspA2I	CCTAGG	2 cut(s)	1216, 1441
AspLEI	GCGC	4 cut(s)	101, 161, 365, 1306
AspS9I	GGNCC	7 cut(s)	350, 521, 640, 917, 1076, 1474, 2005
AsuC2I	CCSGG	2 cut(s)	389, 2567
AsuHPI	GGTGA	1 cut(s)	1047
AsuNHI	GCTAGC	1 cut(s)	2414
AvaII	GGWCC	4 cut(s)	521, 640, 1076, 1474
AvrII	CCTAGG	2 cut(s)	1216, 1441
BaeI	ACNNNNGTAYC	2 cut(s)	694, 727
BalI	TGGCCA	2 cut(s)	1611, 2025
BanI	GGYRCC	1 cut(s)	2151
BauI	CACGAG	1 cut(s)	248
Bbv12I	GWGCWC	2 cut(s)	2120, 2272
BbvI	GCAGC	6 cut(s)	46, 469, 595, 1000, 1033, 1666
BceAI	ACGGC	1 cut(s)	58
BcgI	CGANNNNNNTGC	2 cut(s)	1612, 1646
BciT130I	CCWGG	8 cut(s)	411, 675, 1387, 1829, 1933, 2009, 2149, 2507
BciVI	GTATCC	2 cut(s)	695, 710
BcnI	CCSGG	2 cut(s)	389, 2567
BcoDI	GTCTC	1 cut(s)	632
BfaI	CTAG	5 cut(s)	321, 623, 1217, 1442, 2415
BfmI	CTRYAG	2 cut(s)	536, 1267
BfuAI	ACCTGC	2 cut(s)	1877, 2595
BfuI	GTATCC	2 cut(s)	695, 710
BglI	GCCNNNNNGGC	2 cut(s)	805, 1962
BglII	AGATCT	1 cut(s)	817
BlnI	CCTAGG	2 cut(s)	1216, 1441
BmcAI	AGTACT	1 cut(s)	269
Bme18I	GGWCC	4 cut(s)	521, 640, 1076, 1474
BmgT120I	GGNCC	7 cut(s)	350, 521, 640, 917, 1076, 1474, 2005
BmiI	GGNNCC	7 cut(s)	11, 641, 642, 918, 1077, 2153, 2260
BmrI	ACTGGG	1 cut(s)	1788
BmtI	GCTAGC	1 cut(s)	2418
BmuI	ACTGGG	1 cut(s)	1788
BplI	GAGNNNNNCTC	2 cut(s)	1514, 1546
BpmI	CTGGAG	1 cut(s)	657
BpuEI	CTTGAG	4 cut(s)	1757, 2256, 2415, 2439
BpuMI	CCSGG	2 cut(s)	389, 2567
Bsa29I	ATCGAT	2 cut(s)	111, 1572
BsaBI	GATNNNNATC	1 cut(s)	2237
BsaI	GGTCTC	1 cut(s)	632
BsaWI	WCCGGW	1 cut(s)	1057
BsaXI	ACNNNNNCTCC	2 cut(s)	318, 348
Bsc4I	CCNNNNNNNGG	9 cut(s)	174, 260, 989, 1299, 2014, 2066, 2566, 2567, 2621
Bse118I	RCCGGY	1 cut(s)	140
Bse1I	ACTGG	7 cut(s)	524, 933, 1159, 1346, 1783, 1911, 1973
Bse3DI	GCAATG	2 cut(s)	703, 2381
Bse8I	GATNNNNATC	1 cut(s)	2237
BseBI	CCWGG	8 cut(s)	411, 675, 1387, 1829, 1933, 2009, 2149, 2507
BseCI	ATCGAT	2 cut(s)	111, 1572
BseGI	GGATG	7 cut(s)	144, 220, 772, 776, 1395, 1919, 2075
BseJI	GATNNNNATC	1 cut(s)	2237
BseLI	CCNNNNNNNGG	9 cut(s)	174, 260, 989, 1299, 2014, 2066, 2566, 2567, 2621
BseMI	GCAATG	2 cut(s)	703, 2381
BseMII	CTCAG	5 cut(s)	357, 603, 965, 1715, 1887
BseNI	ACTGG	7 cut(s)	524, 933, 1159, 1346, 1783, 1911, 1973
BseXI	GCAGC	6 cut(s)	46, 469, 595, 1000, 1033, 1666
BseYI	CCCAGC	1 cut(s)	635
BsgI	GTGCAG	2 cut(s)	1673, 2271
Bsh1236I	CGCG	4 cut(s)	50, 99, 159, 2248
BshFI	GGCC	9 cut(s)	178, 246, 352, 808, 903, 919, 1611, 2007, 2025
BshNI	GGYRCC	1 cut(s)	2151
BshVI	ATCGAT	2 cut(s)	111, 1572
BsiHKAI	GWGCWC	2 cut(s)	2120, 2272
BsiSI	CCGG	6 cut(s)	7, 141, 389, 904, 1058, 2566
BslFI	GGGAC	4 cut(s)	416, 626, 1005, 1089
BslI	CCNNNNNNNGG	9 cut(s)	174, 260, 989, 1299, 2014, 2066, 2566, 2567, 2621
BsmAI	GTCTC	1 cut(s)	632
BsmFI	GGGAC	4 cut(s)	416, 626, 1005, 1089
BsmI	GAATGC	1 cut(s)	1169
BsnI	GGCC	9 cut(s)	178, 246, 352, 808, 903, 919, 1611, 2007, 2025
Bso31I	GGTCTC	1 cut(s)	632
Bsp1286I	GDGCHC	2 cut(s)	2120, 2272
Bsp143I	GATC	9 cut(s)	219, 481, 817, 1003, 1196, 1629, 1915, 1969, 2350
Bsp19I	CCATGG	2 cut(s)	644, 1241
BspANI	GGCC	9 cut(s)	178, 246, 352, 808, 903, 919, 1611, 2007, 2025
BspCNI	CTCAG	5 cut(s)	358, 604, 966, 1714, 1888
BspDI	ATCGAT	2 cut(s)	111, 1572
BspFNI	CGCG	4 cut(s)	50, 99, 159, 2248
BspLI	GGNNCC	7 cut(s)	11, 641, 642, 918, 1077, 2153, 2260
BspMI	ACCTGC	2 cut(s)	1877, 2595
BspOI	GCTAGC	1 cut(s)	2418
BspPI	GGATC	5 cut(s)	214, 489, 1011, 1910, 1964
BspQI	GCTCTTC	2 cut(s)	492, 1395
BspT107I	GGYRCC	1 cut(s)	2151
BspTNI	GGTCTC	1 cut(s)	632
BsrBI	CCGCTC	1 cut(s)	243
BsrDI	GCAATG	2 cut(s)	703, 2381
BsrFI	RCCGGY	1 cut(s)	140
BsrI	ACTGG	7 cut(s)	524, 933, 1159, 1346, 1783, 1911, 1973
BssAI	RCCGGY	1 cut(s)	140
BssMI	GATC	9 cut(s)	219, 481, 817, 1003, 1196, 1629, 1915, 1969, 2350
BssNAI	GTATAC	2 cut(s)	1099, 1374
BssSI	CACGAG	1 cut(s)	248
BssT1I	CCWWGG	5 cut(s)	644, 1216, 1241, 1293, 1441
Bst1107I	GTATAC	2 cut(s)	1099, 1374
Bst2BI	CACGAG	1 cut(s)	248
Bst2UI	CCWGG	8 cut(s)	411, 675, 1387, 1829, 1933, 2009, 2149, 2507
Bst4CI	ACNGT	9 cut(s)	267, 710, 1103, 1111, 1268, 1438, 1708, 2166, 2620
Bst6I	CTCTTC	4 cut(s)	444, 492, 1347, 1395
BstDEI	CTNAG	6 cut(s)	366, 612, 974, 1701, 1896, 2631
BstDSI	CCRYGG	4 cut(s)	48, 644, 1241, 2162
BstF5I	GGATG	7 cut(s)	144, 220, 772, 776, 1395, 1919, 2075
BstFNI	CGCG	4 cut(s)	50, 99, 159, 2248
BstHHI	GCGC	4 cut(s)	101, 161, 365, 1306
BstKTI	GATC	9 cut(s)	222, 484, 820, 1006, 1199, 1632, 1918, 1972, 2353
BstMAI	GTCTC	1 cut(s)	632
BstMBI	GATC	9 cut(s)	219, 481, 817, 1003, 1196, 1629, 1915, 1969, 2350
BstNI	CCWGG	8 cut(s)	411, 675, 1387, 1829, 1933, 2009, 2149, 2507
BstNSI	RCATGY	6 cut(s)	130, 696, 1013, 1097, 1582, 1769
BstSFI	CTRYAG	2 cut(s)	536, 1267
BstUI	CGCG	4 cut(s)	50, 99, 159, 2248
BstV1I	GCAGC	6 cut(s)	46, 469, 595, 1000, 1033, 1666
BstX2I	RGATCY	4 cut(s)	481, 817, 1003, 1969
BstXI	CCANNNNNNTGG	2 cut(s)	750, 2169
BstYI	RGATCY	4 cut(s)	481, 817, 1003, 1969
BstZ17I	GTATAC	2 cut(s)	1099, 1374
Bsu15I	ATCGAT	2 cut(s)	111, 1572
BsuI	GTATCC	2 cut(s)	695, 710
BsuRI	GGCC	9 cut(s)	178, 246, 352, 808, 903, 919, 1611, 2007, 2025
BsuTUI	ATCGAT	2 cut(s)	111, 1572
BtgI	CCRYGG	4 cut(s)	48, 644, 1241, 2162
BtsCI	GGATG	7 cut(s)	144, 220, 772, 776, 1395, 1919, 2075
BtsI	GCAGTG	2 cut(s)	1287, 1630
BtsIMutI	CAGTG	4 cut(s)	1166, 1287, 1630, 1904
BveI	ACCTGC	2 cut(s)	1877, 2595
CaiI	CAGNNNCTG	1 cut(s)	1205
CfoI	GCGC	4 cut(s)	101, 161, 365, 1306
Cfr10I	RCCGGY	1 cut(s)	140
Cfr13I	GGNCC	7 cut(s)	350, 521, 640, 917, 1076, 1474, 2005
Cfr42I	CCGCGG	1 cut(s)	51
ClaI	ATCGAT	2 cut(s)	111, 1572
CsiI	ACCWGGT	3 cut(s)	1827, 1931, 2147
Csp6I	GTAC	4 cut(s)	268, 891, 1936, 2152
CviQI	GTAC	4 cut(s)	268, 891, 1936, 2152
DdeI	CTNAG	6 cut(s)	366, 612, 974, 1701, 1896, 2631
DpnI	GATC	9 cut(s)	221, 483, 819, 1005, 1198, 1631, 1917, 1971, 2352
DpnII	GATC	9 cut(s)	219, 481, 817, 1003, 1196, 1629, 1915, 1969, 2350
DraI	TTTAAA	2 cut(s)	1461, 1786
EaeI	YGGCCR	4 cut(s)	244, 901, 1609, 2023
Eam1104I	CTCTTC	4 cut(s)	444, 492, 1347, 1395
EarI	CTCTTC	4 cut(s)	444, 492, 1347, 1395
EciI	GGCGGA	2 cut(s)	127, 227
Eco130I	CCWWGG	5 cut(s)	644, 1216, 1241, 1293, 1441
Eco147I	AGGCCT	1 cut(s)	808
Eco31I	GGTCTC	1 cut(s)	632
Eco32I	GATATC	2 cut(s)	292, 1798
Eco47I	GGWCC	4 cut(s)	521, 640, 1076, 1474
Eco57I	CTGAAG	1 cut(s)	1019
EcoO109I	RGGNCCY	2 cut(s)	350, 640
EcoRI	GAATTC	2 cut(s)	419, 2476
EcoRII	CCWGG	8 cut(s)	409, 673, 1385, 1827, 1931, 2007, 2147, 2505
EcoRV	GATATC	2 cut(s)	292, 1798
EcoT14I	CCWWGG	5 cut(s)	644, 1216, 1241, 1293, 1441
EcoT22I	ATGCAT	3 cut(s)	235, 694, 781
ErhI	CCWWGG	5 cut(s)	644, 1216, 1241, 1293, 1441
FalI	AAGNNNNNCTT	2 cut(s)	1931, 1963
FaqI	GGGAC	4 cut(s)	416, 626, 1005, 1089
FauI	CCCGC	2 cut(s)	200, 393
FauNDI	CATATG	1 cut(s)	574
FblI	GTMKAC	2 cut(s)	1098, 1373
FokI	GGATG	7 cut(s)	131, 227, 779, 783, 1402, 1906, 2082
FspBI	CTAG	5 cut(s)	321, 623, 1217, 1442, 2415
GlaI	GCGC	4 cut(s)	100, 160, 364, 1305
GsaI	CCCAGC	1 cut(s)	639
GsuI	CTGGAG	1 cut(s)	657
HaeIII	GGCC	9 cut(s)	178, 246, 352, 808, 903, 919, 1611, 2007, 2025
HapII	CCGG	6 cut(s)	7, 141, 389, 904, 1058, 2566
HhaI	GCGC	4 cut(s)	101, 161, 365, 1306
Hin6I	GCGC	4 cut(s)	99, 159, 363, 1304
HinP1I	GCGC	4 cut(s)	99, 159, 363, 1304
HincII	GTYRAC	1 cut(s)	1698
HindII	GTYRAC	1 cut(s)	1698
HindIII	AAGCTT	1 cut(s)	1022
HinfI	GANTC	4 cut(s)	327, 1052, 2221, 2282
HpaI	GTTAAC	1 cut(s)	1698
HpaII	CCGG	6 cut(s)	7, 141, 389, 904, 1058, 2566
HphI	GGTGA	1 cut(s)	1047
Hpy166II	GTNNAC	7 cut(s)	326, 980, 1099, 1114, 1327, 1374, 1698
Hpy188III	TCNNGA	4 cut(s)	223, 2302, 2318, 2381
Hpy8I	GTNNAC	7 cut(s)	326, 980, 1099, 1114, 1327, 1374, 1698
HpyAV	CCTTC	7 cut(s)	32, 197, 272, 583, 994, 1876, 2031
HpyCH4III	ACNGT	9 cut(s)	267, 710, 1103, 1111, 1268, 1438, 1708, 2166, 2620
HpyCH4IV	ACGT	5 cut(s)	81, 357, 850, 1275, 2582
HpyF3I	CTNAG	6 cut(s)	366, 612, 974, 1701, 1896, 2631
HpySE526I	ACGT	5 cut(s)	81, 357, 850, 1275, 2582
HspAI	GCGC	4 cut(s)	99, 159, 363, 1304
KflI	GGGWCCC	1 cut(s)	640
KpnI	GGTACC	1 cut(s)	2155
KroI	GCCGGC	1 cut(s)	140
KroNI	GCCGGC	1 cut(s)	142
KspAI	GTTAAC	1 cut(s)	1698
KspI	CCGCGG	1 cut(s)	51
Kzo9I	GATC	9 cut(s)	219, 481, 817, 1003, 1196, 1629, 1915, 1969, 2350
LguI	GCTCTTC	2 cut(s)	492, 1395
LmnI	GCTCC	9 cut(s)	9, 15, 240, 317, 676, 858, 955, 1453, 2062
Lsp1109I	GCAGC	6 cut(s)	46, 469, 595, 1000, 1033, 1666
MabI	ACCWGGT	3 cut(s)	1827, 1931, 2147
MaeI	CTAG	5 cut(s)	321, 623, 1217, 1442, 2415
MaeII	ACGT	5 cut(s)	81, 357, 850, 1275, 2582
MaeIII	GTNAC	4 cut(s)	1207, 1465, 1902, 2607
MalI	GATC	9 cut(s)	221, 483, 819, 1005, 1198, 1631, 1917, 1971, 2352
MbiI	CCGCTC	1 cut(s)	243
MboI	GATC	9 cut(s)	219, 481, 817, 1003, 1196, 1629, 1915, 1969, 2350
MfeI	CAATTG	2 cut(s)	954, 1407
MflI	RGATCY	4 cut(s)	481, 817, 1003, 1969
MhlI	GDGCHC	2 cut(s)	2120, 2272
MlsI	TGGCCA	2 cut(s)	1611, 2025
MluNI	TGGCCA	2 cut(s)	1611, 2025
MlyI	GAGTC	1 cut(s)	321
MmeI	TCCRAC	3 cut(s)	871, 2236, 2509
Mox20I	TGGCCA	2 cut(s)	1611, 2025
Mph1103I	ATGCAT	3 cut(s)	235, 694, 781
MroNI	GCCGGC	1 cut(s)	140
MroXI	GAANNNNTTC	2 cut(s)	2309, 2480
MscI	TGGCCA	2 cut(s)	1611, 2025
MslI	CAYNNNNRTG	3 cut(s)	1461, 1816, 2167
Msp20I	TGGCCA	2 cut(s)	1611, 2025
MspA1I	CMGCKG	3 cut(s)	50, 59, 460
MspI	CCGG	6 cut(s)	7, 141, 389, 904, 1058, 2566
MunI	CAATTG	2 cut(s)	954, 1407
Mva1269I	GAATGC	1 cut(s)	1169
MvaI	CCWGG	8 cut(s)	411, 675, 1387, 1829, 1933, 2009, 2149, 2507
MvnI	CGCG	4 cut(s)	50, 99, 159, 2248
NaeI	GCCGGC	1 cut(s)	142
NciI	CCSGG	2 cut(s)	389, 2567
NcoI	CCATGG	2 cut(s)	644, 1241
NdeI	CATATG	1 cut(s)	574
NdeII	GATC	9 cut(s)	219, 481, 817, 1003, 1196, 1629, 1915, 1969, 2350
NgoMIV	GCCGGC	1 cut(s)	140
NheI	GCTAGC	1 cut(s)	2414
NlaIV	GGNNCC	7 cut(s)	11, 641, 642, 918, 1077, 2153, 2260
NmuCI	GTSAC	3 cut(s)	1465, 1902, 2607
NsiI	ATGCAT	3 cut(s)	235, 694, 781
NspI	RCATGY	6 cut(s)	130, 696, 1013, 1097, 1582, 1769
PaeI	GCATGC	3 cut(s)	130, 696, 1582
PceI	AGGCCT	1 cut(s)	808
PciI	ACATGT	2 cut(s)	1093, 1765
PciSI	GCTCTTC	2 cut(s)	492, 1395
PctI	GAATGC	1 cut(s)	1169
PdiI	GCCGGC	1 cut(s)	142
PdmI	GAANNNNTTC	2 cut(s)	2309, 2480
PfeI	GAWTC	3 cut(s)	1052, 2221, 2282
PflMI	CCANNNNNTGG	2 cut(s)	2066, 2621
PfoI	TCCNGGA	1 cut(s)	673
PleI	GAGTC	1 cut(s)	321
PpsI	GAGTC	1 cut(s)	321
PpuMI	RGGWCCY	1 cut(s)	640
PscI	ACATGT	2 cut(s)	1093, 1765
PsiI	TTATAA	1 cut(s)	2393
Psp1406I	AACGTT	1 cut(s)	1275
Psp5II	RGGWCCY	1 cut(s)	640
Psp6I	CCWGG	8 cut(s)	409, 673, 1385, 1827, 1931, 2007, 2147, 2505
PspFI	CCCAGC	1 cut(s)	635
PspGI	CCWGG	8 cut(s)	409, 673, 1385, 1827, 1931, 2007, 2147, 2505
PspN4I	GGNNCC	7 cut(s)	11, 641, 642, 918, 1077, 2153, 2260
PspPI	GGNCC	7 cut(s)	350, 521, 640, 917, 1076, 1474, 2005
PspPPI	RGGWCCY	1 cut(s)	640
PstNI	CAGNNNCTG	1 cut(s)	1205
PsuI	RGATCY	4 cut(s)	481, 817, 1003, 1969
RsaI	GTAC	4 cut(s)	269, 892, 1937, 2153
RsaNI	GTAC	4 cut(s)	268, 891, 1936, 2152
RseI	CAYNNNNRTG	3 cut(s)	1461, 1816, 2167
SacII	CCGCGG	1 cut(s)	51
SapI	GCTCTTC	2 cut(s)	492, 1395
Sau3AI	GATC	9 cut(s)	219, 481, 817, 1003, 1196, 1629, 1915, 1969, 2350
Sau96I	GGNCC	7 cut(s)	350, 521, 640, 917, 1076, 1474, 2005
ScaI	AGTACT	1 cut(s)	269
SchI	GAGTC	1 cut(s)	321
SduI	GDGCHC	2 cut(s)	2120, 2272
SexAI	ACCWGGT	3 cut(s)	1827, 1931, 2147
SfcI	CTRYAG	2 cut(s)	536, 1267
Sfr303I	CCGCGG	1 cut(s)	51
SgrBI	CCGCGG	1 cut(s)	51
SinI	GGWCC	4 cut(s)	521, 640, 1076, 1474
SmiI	ATTTAAAT	1 cut(s)	1461
SmiMI	CAYNNNNRTG	3 cut(s)	1461, 1816, 2167
SmlI	CTYRAG	4 cut(s)	1736, 2271, 2430, 2454
SmoI	CTYRAG	4 cut(s)	1736, 2271, 2430, 2454
SphI	GCATGC	3 cut(s)	130, 696, 1582
SseBI	AGGCCT	1 cut(s)	808
SspI	AATATT	3 cut(s)	1449, 1509, 1552
SspMI	CTAG	5 cut(s)	321, 623, 1217, 1442, 2415
StuI	AGGCCT	1 cut(s)	808
StyI	CCWWGG	5 cut(s)	644, 1216, 1241, 1293, 1441
SwaI	ATTTAAAT	1 cut(s)	1461
TaaI	ACNGT	9 cut(s)	267, 710, 1103, 1111, 1268, 1438, 1708, 2166, 2620
TaiI	ACGT	5 cut(s)	84, 360, 853, 1278, 2585
TaqI	TCGA	4 cut(s)	111, 1572, 1800, 2092
TaqII	GACCGA	1 cut(s)	1622
TatI	WGTACW	1 cut(s)	267
TauI	GCSGC	5 cut(s)	50, 59, 126, 246, 256
TfiI	GAWTC	3 cut(s)	1052, 2221, 2282
TscAI	CASTG	4 cut(s)	1166, 1294, 1630, 1911
TseFI	GTSAC	3 cut(s)	1465, 1902, 2607
TseI	GCWGC	6 cut(s)	59, 457, 608, 1013, 1046, 1654
Tsp45I	GTSAC	3 cut(s)	1465, 1902, 2607
TspDTI	ATGAA	6 cut(s)	564, 720, 975, 1203, 1394, 2346
TspRI	CASTG	4 cut(s)	1166, 1294, 1630, 1911
Van91I	CCANNNNNTGG	2 cut(s)	2066, 2621
VpaK11BI	GGWCC	4 cut(s)	521, 640, 1076, 1474
XapI	RAATTY	7 cut(s)	419, 727, 1366, 1984, 2364, 2408, 2476
XceI	RCATGY	6 cut(s)	130, 696, 1013, 1097, 1582, 1769
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1413
XmaJI	CCTAGG	2 cut(s)	1216, 1441
XmiI	GTMKAC	2 cut(s)	1098, 1373
XmnI	GAANNNNTTC	2 cut(s)	2309, 2480
XspI	CTAG	5 cut(s)	321, 623, 1217, 1442, 2415
ZrmI	AGTACT	1 cut(s)	269
Zsp2I	ATGCAT	3 cut(s)	235, 694, 781

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.