AT2G34530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004483
Physical Location & Seq
Reverse (-)
14548905 .. 14550354
1450 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G34530.3

Sequence Viewer

Length: 849 bp
ATGAAAACCAAGTATGGAGCAGCAGTTGCACCTGTATTCCTCCTTGCTGCTGCATATGTCGCTTGGAACTACATAAATCGTGGTTTATGGAGAAAGAAAGACGATGAAAATGGTCGAGATACAAGAAAAAGCATCGGTAAAAACATTTCAGAACAAAGAAGAGATGGTAATGTTAAAAACATGGGTAAGGAGGCTTCTTCAGGAAGAGATGAAAGAACCCTCTCAAAATCTGTTTCAATGGGAGCTATTCGAGGAGGAAAATTAGCACTACAGAGATTGCTTGATTTGCACTCATACCGCGGTGACACTTCTTCCCTTGCGAATGCTGAACTCGAATTTGAAGCATTGCTTTCCAAGGAAAAGCCGGACTTCGGGTTGCTTCAGAGAGACATTGTAAAAATGGAAATGAGTGGAAAAGAAGCAAAAGGAGCTGAGCTATTGAAAAAGGCATTGGAGAAAGCAAGAAAAGAAGGGAAAGGTCATGAGGCTTATGAGATTGAGATGTTGCTTGTGGAGATGTTGATCTACTTGGGAAACATGGAAGAGGCTTCAAAATGTAAGTGTTTAGAGGATGAAGTCATAACAGATGCAAGACGTCCTCTATATCAGACTATAATCCATTACCTTCAAGGTCATCCTATGAAGCAAGTCGAGGAGACATTCAACAGGTTCAGAGAGATTCAAATTGGTCTACAATGGCCTGGAAGCTCAGAAGAATGTGAAATTGACCAAGTCACATTTGATGAGTTCAAGAAAGTTATGGAATCTCTCAAGCATGAAATCGAAGACGGCAACAAAAGAAAGTTGAAGTTAGTCAAAGATAAAACTATGAGAGATAGTCTTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

282

Amino Acids

32.23

Weight (kDa)

8.53

Isoelectric Point (pI)

37.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 598
AccI GTMKAC 1 cut(s) 691
AccII CGCG 1 cut(s) 300
AciI CCGC 2 cut(s) 298, 300
AcsI RAATTY 1 cut(s) 335
AcuI CTGAAG 2 cut(s) 183, 365
AcyI GRCGYC 1 cut(s) 595
AfiI CCNNNNNNNGG 1 cut(s) 371
AgsI TTSAA 9 cut(s) 237, 341, 442, 552, 629, 664, 683, 751, 808
AjnI CCWGG 1 cut(s) 700
AjuI GAANNNNNNNTTGG 2 cut(s) 434, 466
AluBI AGCT 4 cut(s) 245, 431, 436, 708
AluI AGCT 4 cut(s) 245, 431, 436, 708
Alw26I GTCTC 2 cut(s) 381, 650
AoxI GGCC 1 cut(s) 698
ApeKI GCWGC 3 cut(s) 20, 47, 50
ApoI RAATTY 1 cut(s) 335
AsuHPI GGTGA 1 cut(s) 314
BarI GAAGNNNNNNTAC 2 cut(s) 178, 210
BbsI GAAGAC 2 cut(s) 792, 833
BbvI GCAGC 3 cut(s) 32, 34, 37
BccI CCATC 1 cut(s) 158
BceAI ACGGC 1 cut(s) 805
BciT130I CCWGG 1 cut(s) 702
BcoDI GTCTC 2 cut(s) 381, 650
BfmI CTRYAG 1 cut(s) 269
BisI GCNGC 3 cut(s) 21, 48, 51
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 3 cut(s) 22, 49, 52
Bme1390I CCNGG 1 cut(s) 702
BmrFI CCNGG 1 cut(s) 702
BmsI GCATC 2 cut(s) 141, 577
BpiI GAAGAC 2 cut(s) 792, 833
Bpu1102I GCTNAGC 1 cut(s) 432
BpuEI CTTGAG 1 cut(s) 755
BsaBI GATNNNNATC 1 cut(s) 521
BsaHI GRCGYC 1 cut(s) 595
BsaJI CCNNGG 2 cut(s) 298, 354
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 1 cut(s) 371
Bse3DI GCAATG 1 cut(s) 344
Bse8I GATNNNNATC 1 cut(s) 521
BseBI CCWGG 1 cut(s) 702
BseDI CCNNGG 2 cut(s) 298, 354
BseGI GGATG 2 cut(s) 577, 634
BseJI GATNNNNATC 1 cut(s) 521
BseLI CCNNNNNNNGG 1 cut(s) 371
BseMI GCAATG 1 cut(s) 344
BseMII CTCAG 2 cut(s) 423, 723
BseRI GAGGAG 2 cut(s) 267, 668
BseXI GCAGC 3 cut(s) 32, 34, 37
Bsh1236I CGCG 1 cut(s) 300
BshFI GGCC 1 cut(s) 700
BsiSI CCGG 1 cut(s) 365
BslI CCNNNNNNNGG 1 cut(s) 371
BsmAI GTCTC 2 cut(s) 381, 650
BsmI GAATGC 1 cut(s) 328
BsnI GGCC 1 cut(s) 700
Bsp143I GATC 1 cut(s) 522
Bsp1720I GCTNAGC 1 cut(s) 432
BspACI CCGC 2 cut(s) 298, 300
BspANI GGCC 1 cut(s) 700
BspCNI CTCAG 2 cut(s) 424, 722
BspFNI CGCG 1 cut(s) 300
BspHI TCATGA 1 cut(s) 481
BsrDI GCAATG 1 cut(s) 344
BssECI CCNNGG 2 cut(s) 298, 354
BssMI GATC 1 cut(s) 522
BssNI GRCGYC 1 cut(s) 595
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 702
Bst6I CTCTTC 3 cut(s) 154, 199, 537
BstACI GRCGYC 1 cut(s) 595
BstAPI GCANNNNNTGC 1 cut(s) 26
BstDEI CTNAG 2 cut(s) 432, 709
BstDSI CCRYGG 1 cut(s) 298
BstF5I GGATG 2 cut(s) 577, 634
BstFNI CGCG 1 cut(s) 300
BstKTI GATC 1 cut(s) 525
BstMAI GTCTC 2 cut(s) 381, 650
BstMBI GATC 1 cut(s) 522
BstMWI GCNNNNNNNGC 4 cut(s) 26, 59, 286, 428
BstNI CCWGG 1 cut(s) 702
BstSCI CCNGG 1 cut(s) 700
BstSFI CTRYAG 1 cut(s) 269
BstUI CGCG 1 cut(s) 300
BstV1I GCAGC 3 cut(s) 32, 34, 37
BstV2I GAAGAC 2 cut(s) 792, 833
BsuRI GGCC 1 cut(s) 700
BtgI CCRYGG 1 cut(s) 298
BtsCI GGATG 2 cut(s) 577, 634
CciI TCATGA 1 cut(s) 481
Cfr42I CCGCGG 1 cut(s) 301
CviAII CATG 4 cut(s) 181, 482, 538, 776
CviJI RGCY 9 cut(s) 194, 245, 364, 431, 436, 488, 548, 700, 708
CviKI_1 RGCY 9 cut(s) 194, 245, 364, 431, 436, 488, 548, 700, 708
DdeI CTNAG 2 cut(s) 432, 709
DpnI GATC 1 cut(s) 524
DpnII GATC 1 cut(s) 522
Eam1104I CTCTTC 3 cut(s) 154, 199, 537
EarI CTCTTC 3 cut(s) 154, 199, 537
Eco130I CCWWGG 1 cut(s) 354
Eco57I CTGAAG 2 cut(s) 183, 365
EcoRII CCWGG 1 cut(s) 700
EcoT14I CCWWGG 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 4 cut(s) 184, 485, 541, 779
FalI AAGNNNNNCTT 4 cut(s) 333, 365, 353, 385
FatI CATG 4 cut(s) 180, 481, 537, 775
FauNDI CATATG 1 cut(s) 55
FblI GTMKAC 1 cut(s) 691
Fnu4HI GCNGC 3 cut(s) 21, 48, 51
FokI GGATG 2 cut(s) 584, 621
Fsp4HI GCNGC 3 cut(s) 21, 48, 51
GluI GCNGC 3 cut(s) 21, 48, 51
HaeIII GGCC 1 cut(s) 700
HapII CCGG 1 cut(s) 365
Hin1I GRCGYC 1 cut(s) 595
Hin1II CATG 4 cut(s) 184, 485, 541, 779
HinfI GANTC 2 cut(s) 679, 764
HpaII CCGG 1 cut(s) 365
HphI GGTGA 1 cut(s) 314
Hpy166II GTNNAC 1 cut(s) 692
Hpy188I TCNGA 5 cut(s) 151, 384, 609, 674, 712
Hpy188III TCNNGA 4 cut(s) 116, 201, 482, 751
Hpy8I GTNNAC 1 cut(s) 692
HpyAV CCTTC 2 cut(s) 464, 635
HpyCH4IV ACGT 1 cut(s) 595
HpyCH4V TGCA 4 cut(s) 29, 53, 289, 590
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 59, 286, 428
HpyF3I CTNAG 2 cut(s) 432, 709
HpySE526I ACGT 1 cut(s) 595
Hsp92I GRCGYC 1 cut(s) 595
Hsp92II CATG 4 cut(s) 184, 485, 541, 779
KspI CCGCGG 1 cut(s) 301
Kzo9I GATC 1 cut(s) 522
LmnI GCTCC 3 cut(s) 17, 242, 428
LpnPI CCDG 6 cut(s) 45, 186, 378, 652, 687, 714
Lsp1109I GCAGC 3 cut(s) 32, 34, 37
LweI GCATC 2 cut(s) 141, 577
MaeII ACGT 1 cut(s) 595
MaeIII GTNAC 2 cut(s) 302, 733
MalI GATC 1 cut(s) 524
MboI GATC 1 cut(s) 522
MboII GAAGA 8 cut(s) 171, 189, 216, 303, 554, 725, 797, 833
MluCI AATT 4 cut(s) 260, 335, 684, 723
MseI TTAA 2 cut(s) 174, 847
MspA1I CMGCKG 1 cut(s) 300
MspI CCGG 1 cut(s) 365
MspR9I CCNGG 1 cut(s) 702
Mva1269I GAATGC 1 cut(s) 328
MvaI CCWGG 1 cut(s) 702
MvnI CGCG 1 cut(s) 300
MwoI GCNNNNNNNGC 4 cut(s) 26, 59, 286, 428
NdeI CATATG 1 cut(s) 55
NdeII GATC 1 cut(s) 522
NlaIII CATG 4 cut(s) 184, 485, 541, 779
NmuCI GTSAC 2 cut(s) 302, 733
PagI TCATGA 1 cut(s) 481
PctI GAATGC 1 cut(s) 328
PfeI GAWTC 2 cut(s) 679, 764
PflFI GACNNNGTC 1 cut(s) 731
PkrI GCNGC 3 cut(s) 22, 49, 52
Psp6I CCWGG 1 cut(s) 700
PspGI CCWGG 1 cut(s) 700
PsyI GACNNNGTC 1 cut(s) 731
SacII CCGCGG 1 cut(s) 301
SaqAI TTAA 2 cut(s) 174, 847
SatI GCNGC 3 cut(s) 21, 48, 51
Sau3AI GATC 1 cut(s) 522
ScrFI CCNGG 1 cut(s) 702
SfaNI GCATC 2 cut(s) 141, 577
SfcI CTRYAG 1 cut(s) 269
Sfr303I CCGCGG 1 cut(s) 301
SgrBI CCGCGG 1 cut(s) 301
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
Sse9I AATT 4 cut(s) 260, 335, 684, 723
SsiI CCGC 2 cut(s) 298, 300
StyD4I CCNGG 1 cut(s) 700
StyI CCWWGG 1 cut(s) 354
TaiI ACGT 1 cut(s) 598
TaqI TCGA 5 cut(s) 115, 250, 333, 651, 783
TasI AATT 4 cut(s) 260, 335, 684, 723
TfiI GAWTC 2 cut(s) 679, 764
Tru1I TTAA 2 cut(s) 174, 847
Tru9I TTAA 2 cut(s) 174, 847
TseFI GTSAC 2 cut(s) 302, 733
TseI GCWGC 3 cut(s) 20, 47, 50
Tsp45I GTSAC 2 cut(s) 302, 733
TspDTI ATGAA 7 cut(s) 17, 120, 225, 588, 656, 792, 833
Tth111I GACNNNGTC 1 cut(s) 731
XapI RAATTY 1 cut(s) 335
XmiI GTMKAC 1 cut(s) 691
ZraI GACGTC 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.