AT2G36660

polyadenylate-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0010442
Physical Location & Seq
Reverse (-)
15361321 .. 15364398
3078 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G36660.1

Sequence Viewer

Length: 1830 bp
ATGGCGACTGTTCATGCTGCATTACATGCGGCGGATGCTTCTTCTTCGGGATCGAGTTCTCCAGTGACGGCGTCGTTATATGTCGGAGACTTACACCCTTCCGTCACCGAAGGTATACTCTACGACGCGTTCGCCGAGTTCAAGAGCTTAACTTCTGTTCGTCTCTGTAAAGACGCTTCGTCTGGTCGCTCTCTCTGTTATGGCTACGCTAACTTTCTCTCGCGTCAAGACGCTAACCTTGCCATTGAGAAAAAAAATAACAGTTTGTTGAATGGAAAAATGATTCGAGTAATGTGGTCTGTTCGAGCACCTGATGCTAGGAGAAATGGGGTTGGAAATGTCTTTGTTAAGAATTTGCCTGAGTCAGTCACCAATGCGGTTCTGCAAGATATGTTCAAGAAATTTGGGAACATTGTTTCCTGTAAGGTTGCAACTCTTGAAGATGGAAAGAGTCGGGGATATGGTTTTGTTCAATTTGAGCAAGAAGACGCTGCACATGCTGCTATTCAGACCTTGAATAGTACCATTGTTGCTGACAAGGAGATCTATGTTGGAAAATTCATGAAAAAGACTGATCGAGTGAAACCTGAGGAAAAGTACACGAACTTGTACATGAAGAATCTCGATGCAGATGTCAGTGAGGATCTTTTAAGGGAAAAGTTTGCAGAGTTTGGGAAAATTGTTAGCCTAGCAATTGCAAAAGATGAGAACCGATTATGTAGAGGATATGCATTTGTCAACTTTGACAACCCAGAGGATGCAAGACGGGCAGCTGAAACAGTGAATGGAACAAAATTTGGTTCGAAGTGTCTATATGTAGGAAGGGCGCAAAAGAAAGCAGAACGGGAGCAACTGCTAAGGGAACAATTTAAAGAGAAACATGAAGAACAGAAGATGATAGCCAAGGTGTCAAACATATATGTGAAAAACGTCAATGTTGCTGTTACTGAGGAGGAGCTGAGAAAACACTTCAGTCAATGTGGCACAATCACTTCGACAAAGCTGATGTGTGATGAGAAAGGAAAAAGCAAGGGGTTTGGATTTGTGTGCTTCTCAACACCTGAAGAAGCTATTGATGCTGTGAAAACCTTTCATGGGCAAATGTTTCACGGGAAACCACTATATGTGGCTATTGCTCAAAAGAAAGAGGACAGAAAGATGCAGTTACAGGTTCAATTTGGGAACCGTGTGGAAGCTAGAAAAAGCAGCTCTTCAGCTTCTGTCAATCCAGGGACATACGCTCCACTTTACTACACAAACACTCATCCCGGGATGGTATATCAATCATACCCGCTGATGTGGAAGTCTGCTAACATGATTGGTTCATCATACCCAAACTCTGAAGCAGTCACATATCCCCCAATGGTTGCTAATGCGCCAAGTAAGAATAGGCAGAACAGAATAGGAAAGTTGGATAGGAATGCTGTTTCGTATGTGCCAAATGTGTATCAGTCTACTCAAATGCTTCCTTTGTCAAGAGATTTTTCGAAGCAGCAGCATAGCAGAACCTATGGTCGTGGGAAAGAAATGAAGAAATCAATCCAACAGAGACAGTCTGAAACAGTTGGGATGGAGATGCAATTGCTCGGAGAGTTGCTACACCCTCTTGTCGAGAAACTGGAGCCCCAGCTTGCAAACAAAATCACAGGGATGCTTCTGGAAATGGACAAGTCGGAATTGCTTCTTTTGCTGAAATCACCAGAAGACTTGGCTGTTAGAGTTGATGAAGCGTTTGAAGTTCTGAAATCATCCAAGACAAATCTGACCGCTCCAAACACTCATCGTTCTGATTACTTGGCGTCTGGCATTGCTGGCGTTTCGATTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03018 map03013 map03015 map03018
Pfam Domains
Protein Families
PABP RRM_1

Protein Analysis

609

Amino Acids

67.92

Weight (kDa)

9.18

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 26 - 95 4.5e-12 RNA recognition motif
RRM_1 PF00076 114 - 182 1.4e-21 RNA recognition motif
RRM_1 PF00076 203 - 271 3e-19 RNA recognition motif
RRM_1 PF00076 306 - 374 8.2e-20 RNA recognition motif
MLLE PF00658 526 - 581 3.9e-20 MLLE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1769
AccI GTMKAC 2 cut(s) 115, 1454
AccII CGCG 2 cut(s) 128, 223
AciI CCGC 5 cut(s) 29, 32, 377, 1292, 1767
AclWI GGATC 2 cut(s) 58, 651
AcsI RAATTY 4 cut(s) 352, 401, 557, 794
AcuI CTGAAG 4 cut(s) 955, 1083, 1197, 1362
AcyI GRCGYC 2 cut(s) 71, 1799
AfaI GTAC 3 cut(s) 523, 599, 611
AfiI CCNNNNNNNGG 1 cut(s) 1095
AflIII ACRYGT 1 cut(s) 126
AgsI TTSAA 8 cut(s) 142, 271, 397, 440, 473, 517, 1175, 1736
AhdI GACNNNNNGTC 1 cut(s) 178
AjnI CCWGG 1 cut(s) 1228
AloI GAACNNNNNNTCC 2 cut(s) 401, 433
AluBI AGCT 9 cut(s) 147, 773, 958, 1003, 1070, 1196, 1209, 1217, 1630
AluI AGCT 9 cut(s) 147, 773, 958, 1003, 1070, 1196, 1209, 1217, 1630
Alw21I GWGCWC 1 cut(s) 310
Alw26I GTCTC 3 cut(s) 81, 167, 1543
AlwI GGATC 2 cut(s) 58, 651
AlwNI CAGNNNCTG 1 cut(s) 1220
Ama87I CYCGRG 1 cut(s) 1268
ApeKI GCWGC 7 cut(s) 17, 491, 500, 770, 1206, 1492, 1495
ApoI RAATTY 4 cut(s) 352, 401, 557, 794
Asp700I GAANNNNTTC 1 cut(s) 1680
AspLEI GCGC 2 cut(s) 829, 1378
AsuC2I CCSGG 2 cut(s) 1269, 1270
AsuHPI GGTGA 3 cut(s) 97, 361, 1689
AsuII TTCGAA 2 cut(s) 803, 1487
AvaI CYCGRG 1 cut(s) 1268
AxyI CCTNAGG 1 cut(s) 588
BanII GRGCYC 1 cut(s) 1626
BarI GAAGNNNNNNTAC 2 cut(s) 160, 192
BbsI GAAGAC 2 cut(s) 492, 1710
Bbv12I GWGCWC 1 cut(s) 310
BbvI GCAGC 7 cut(s) 4, 478, 487, 782, 1218, 1504, 1507
BccI CCATC 3 cut(s) 437, 1267, 1564
BceAI ACGGC 1 cut(s) 84
BciT130I CCWGG 1 cut(s) 1230
BcnI CCSGG 2 cut(s) 1269, 1270
BcoDI GTCTC 3 cut(s) 81, 167, 1543
BfaI CTAG 3 cut(s) 318, 689, 1197
BglII AGATCT 1 cut(s) 543
BisI GCNGC 8 cut(s) 18, 30, 492, 501, 771, 1207, 1493, 1496
BlsI GCNGC 8 cut(s) 19, 31, 493, 502, 772, 1208, 1494, 1497
Bme1390I CCNGG 3 cut(s) 1230, 1269, 1270
BmeRI GACNNNNNGTC 1 cut(s) 178
BmeT110I CYCGRG 1 cut(s) 1268
BmiI GGNNCC 2 cut(s) 1184, 1623
BmrFI CCNGG 3 cut(s) 1230, 1269, 1270
BmsI GCATC 8 cut(s) 25, 304, 616, 748, 1066, 1149, 1566, 1641
BpiI GAAGAC 2 cut(s) 492, 1710
BpmI CTGGAG 2 cut(s) 45, 1640
Bpu10I CCTNAGC 1 cut(s) 857
Bpu14I TTCGAA 2 cut(s) 803, 1487
BpuMI CCSGG 2 cut(s) 1269, 1270
BsaHI GRCGYC 2 cut(s) 71, 1799
BsaJI CCNNGG 3 cut(s) 903, 1229, 1268
BsaXI ACNNNNNCTCC 2 cut(s) 1225, 1255
Bsc4I CCNNNNNNNGG 1 cut(s) 1095
Bse1I ACTGG 2 cut(s) 62, 1623
Bse21I CCTNAGG 1 cut(s) 588
Bse3DI GCAATG 1 cut(s) 1806
BseBI CCWGG 1 cut(s) 1230
BseDI CCNNGG 3 cut(s) 903, 1229, 1268
BseGI GGATG 7 cut(s) 40, 763, 1264, 1278, 1575, 1656, 1748
BseLI CCNNNNNNNGG 1 cut(s) 1095
BseMI GCAATG 1 cut(s) 1806
BseMII CTCAG 4 cut(s) 351, 579, 939, 950
BseNI ACTGG 2 cut(s) 62, 1623
BseRI GAGGAG 2 cut(s) 965, 968
BseXI GCAGC 7 cut(s) 4, 478, 487, 782, 1218, 1504, 1507
BseYI CCCAGC 1 cut(s) 1626
BsgI GTGCAG 1 cut(s) 477
Bsh1236I CGCG 2 cut(s) 128, 223
BsiHKAI GWGCWC 1 cut(s) 310
BsiHKCI CYCGRG 1 cut(s) 1268
BsiSI CCGG 1 cut(s) 1269
BslFI GGGAC 1 cut(s) 1246
BslI CCNNNNNNNGG 1 cut(s) 1095
BsmAI GTCTC 3 cut(s) 81, 167, 1543
BsmBI CGTCTC 1 cut(s) 167
BsmFI GGGAC 1 cut(s) 1246
BsmI GAATGC 1 cut(s) 1426
BsoBI CYCGRG 1 cut(s) 1268
Bsp119I TTCGAA 2 cut(s) 803, 1487
Bsp1286I GDGCHC 2 cut(s) 310, 1626
Bsp1407I TGTACA 1 cut(s) 609
Bsp143I GATC 4 cut(s) 50, 543, 574, 643
BspACI CCGC 5 cut(s) 29, 32, 377, 1292, 1767
BspCNI CTCAG 4 cut(s) 352, 580, 940, 951
BspFNI CGCG 2 cut(s) 128, 223
BspHI TCATGA 1 cut(s) 561
BspLI GGNNCC 2 cut(s) 1184, 1623
BspPI GGATC 2 cut(s) 58, 651
BspQI GCTCTTC 1 cut(s) 1216
BspT104I TTCGAA 2 cut(s) 803, 1487
BsrBI CCGCTC 1 cut(s) 1769
BsrDI GCAATG 1 cut(s) 1806
BsrGI TGTACA 1 cut(s) 609
BsrI ACTGG 2 cut(s) 62, 1623
BssECI CCNNGG 3 cut(s) 903, 1229, 1268
BssMI GATC 4 cut(s) 50, 543, 574, 643
BssNAI GTATAC 1 cut(s) 116
BssNI GRCGYC 2 cut(s) 71, 1799
BssT1I CCWWGG 1 cut(s) 903
Bst1107I GTATAC 1 cut(s) 116
Bst2UI CCWGG 1 cut(s) 1230
Bst4CI ACNGT 6 cut(s) 10, 263, 781, 1187, 1554, 1564
Bst6I CTCTTC 1 cut(s) 1216
BstACI GRCGYC 2 cut(s) 71, 1799
BstAPI GCANNNNNTGC 3 cut(s) 26, 314, 500
BstAUI TGTACA 1 cut(s) 609
BstBI TTCGAA 2 cut(s) 803, 1487
BstC8I GCNNGC 2 cut(s) 1632, 1813
BstDEI CTNAG 5 cut(s) 360, 588, 857, 948, 959
BstF5I GGATG 7 cut(s) 40, 763, 1264, 1278, 1575, 1656, 1748
BstFNI CGCG 2 cut(s) 128, 223
BstHHI GCGC 2 cut(s) 829, 1378
BstKTI GATC 4 cut(s) 53, 546, 577, 646
BstMAI GTCTC 3 cut(s) 81, 167, 1543
BstMBI GATC 4 cut(s) 50, 543, 574, 643
BstNI CCWGG 1 cut(s) 1230
BstNSI RCATGY 2 cut(s) 29, 500
BstSCI CCNGG 3 cut(s) 1228, 1267, 1268
BstUI CGCG 2 cut(s) 128, 223
BstV1I GCAGC 7 cut(s) 4, 478, 487, 782, 1218, 1504, 1507
BstV2I GAAGAC 2 cut(s) 492, 1710
BstX2I RGATCY 2 cut(s) 543, 643
BstYI RGATCY 2 cut(s) 543, 643
BstZ17I GTATAC 1 cut(s) 116
Bsu36I CCTNAGG 1 cut(s) 588
BtsCI GGATG 7 cut(s) 40, 763, 1264, 1278, 1575, 1656, 1748
BtsIMutI CAGTG 3 cut(s) 69, 643, 786
Cac8I GCNNGC 2 cut(s) 1632, 1813
CaiI CAGNNNCTG 1 cut(s) 1220
CciI TCATGA 1 cut(s) 561
CfoI GCGC 2 cut(s) 829, 1378
Cfr9I CCCGGG 1 cut(s) 1268
CseI GACGC 7 cut(s) 60, 134, 182, 212, 239, 497, 1788
Csp6I GTAC 3 cut(s) 522, 598, 610
CviAII CATG 8 cut(s) 14, 26, 497, 562, 613, 881, 1094, 1315
CviQI GTAC 3 cut(s) 522, 598, 610
DdeI CTNAG 5 cut(s) 360, 588, 857, 948, 959
DpnI GATC 4 cut(s) 52, 545, 576, 645
DpnII GATC 4 cut(s) 50, 543, 574, 643
DraI TTTAAA 1 cut(s) 871
DriI GACNNNNNGTC 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 1216
Eam1105I GACNNNNNGTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 1216
EciI GGCGGA 1 cut(s) 47
Eco130I CCWWGG 1 cut(s) 903
Eco24I GRGCYC 1 cut(s) 1626
Eco57I CTGAAG 4 cut(s) 955, 1083, 1197, 1362
Eco81I CCTNAGG 1 cut(s) 588
Eco88I CYCGRG 1 cut(s) 1268
EcoRII CCWGG 1 cut(s) 1228
EcoT14I CCWWGG 1 cut(s) 903
EcoT22I ATGCAT 1 cut(s) 733
EcoT38I GRGCYC 1 cut(s) 1626
ErhI CCWWGG 1 cut(s) 903
Esp3I CGTCTC 1 cut(s) 167
FaeI CATG 8 cut(s) 17, 29, 500, 565, 616, 884, 1097, 1318
FalI AAGNNNNNCTT 2 cut(s) 1195, 1227
FaqI GGGAC 1 cut(s) 1246
FatI CATG 8 cut(s) 13, 25, 496, 561, 612, 880, 1093, 1314
FauI CCCGC 1 cut(s) 1299
FblI GTMKAC 2 cut(s) 115, 1454
Fnu4HI GCNGC 8 cut(s) 18, 30, 492, 501, 771, 1207, 1493, 1496
FokI GGATG 7 cut(s) 47, 770, 1251, 1285, 1582, 1663, 1735
FriOI GRGCYC 1 cut(s) 1626
Fsp4HI GCNGC 8 cut(s) 18, 30, 492, 501, 771, 1207, 1493, 1496
FspBI CTAG 3 cut(s) 318, 689, 1197
GlaI GCGC 2 cut(s) 828, 1377
GluI GCNGC 8 cut(s) 18, 30, 492, 501, 771, 1207, 1493, 1496
GsaI CCCAGC 1 cut(s) 1630
GsuI CTGGAG 2 cut(s) 45, 1640
HapII CCGG 1 cut(s) 1269
HgaI GACGC 7 cut(s) 60, 134, 182, 212, 239, 497, 1788
HhaI GCGC 2 cut(s) 829, 1378
Hin1I GRCGYC 2 cut(s) 71, 1799
Hin1II CATG 8 cut(s) 17, 29, 500, 565, 616, 884, 1097, 1318
Hin6I GCGC 2 cut(s) 827, 1376
HinP1I GCGC 2 cut(s) 827, 1376
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HinfI GANTC 4 cut(s) 283, 362, 451, 619
HpaII CCGG 1 cut(s) 1269
HphI GGTGA 3 cut(s) 97, 361, 1689
Hpy166II GTNNAC 4 cut(s) 116, 600, 739, 1455
Hpy188I TCNGA 9 cut(s) 86, 510, 1342, 1558, 1589, 1675, 1743, 1764, 1789
Hpy8I GTNNAC 4 cut(s) 116, 600, 739, 1455
Hpy99I CGWCG 2 cut(s) 76, 128
HpyAV CCTTC 3 cut(s) 104, 108, 816
HpyCH4III ACNGT 6 cut(s) 10, 263, 781, 1187, 1554, 1564
HpyCH4IV ACGT 1 cut(s) 930
HpyF3I CTNAG 5 cut(s) 360, 588, 857, 948, 959
HpySE526I ACGT 1 cut(s) 930
Hsp92I GRCGYC 2 cut(s) 71, 1799
Hsp92II CATG 8 cut(s) 17, 29, 500, 565, 616, 884, 1097, 1318
HspAI GCGC 2 cut(s) 827, 1376
Kzo9I GATC 4 cut(s) 50, 543, 574, 643
LguI GCTCTTC 1 cut(s) 1216
LmnI GCTCC 5 cut(s) 847, 955, 1246, 1621, 1774
Lsp1109I GCAGC 7 cut(s) 4, 478, 487, 782, 1218, 1504, 1507
LweI GCATC 8 cut(s) 25, 304, 616, 748, 1066, 1149, 1566, 1641
MaeI CTAG 3 cut(s) 318, 689, 1197
MaeII ACGT 1 cut(s) 930
MaeIII GTNAC 6 cut(s) 64, 103, 367, 943, 1164, 1348
MalI GATC 4 cut(s) 52, 545, 576, 645
MbiI CCGCTC 1 cut(s) 1769
MboI GATC 4 cut(s) 50, 543, 574, 643
MfeI CAATTG 2 cut(s) 693, 1580
MflI RGATCY 2 cut(s) 543, 643
MhlI GDGCHC 2 cut(s) 310, 1626
MluI ACGCGT 1 cut(s) 126
MlyI GAGTC 2 cut(s) 371, 460
MmeI TCCRAC 6 cut(s) 64, 313, 532, 1392, 1567, 1653
MnlI CCTC 8 cut(s) 583, 634, 716, 748, 943, 946, 1141, 1614
Mph1103I ATGCAT 1 cut(s) 733
MroXI GAANNNNTTC 1 cut(s) 1680
MseI TTAA 5 cut(s) 149, 348, 650, 870, 1824
MslI CAYNNNNRTG 2 cut(s) 920, 1649
MspA1I CMGCKG 2 cut(s) 773, 1294
MspI CCGG 1 cut(s) 1269
MspR9I CCNGG 3 cut(s) 1230, 1269, 1270
MunI CAATTG 2 cut(s) 693, 1580
Mva1269I GAATGC 1 cut(s) 1426
MvaI CCWGG 1 cut(s) 1230
MvnI CGCG 2 cut(s) 128, 223
NciI CCSGG 2 cut(s) 1269, 1270
NdeII GATC 4 cut(s) 50, 543, 574, 643
NlaIII CATG 8 cut(s) 17, 29, 500, 565, 616, 884, 1097, 1318
NlaIV GGNNCC 2 cut(s) 1184, 1623
NmeAIII GCCGAG 1 cut(s) 160
NmuCI GTSAC 4 cut(s) 64, 103, 367, 1348
NsiI ATGCAT 1 cut(s) 733
NspI RCATGY 2 cut(s) 29, 500
NspV TTCGAA 2 cut(s) 803, 1487
PagI TCATGA 1 cut(s) 561
PciSI GCTCTTC 1 cut(s) 1216
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PctI GAATGC 1 cut(s) 1426
PdmI GAANNNNTTC 1 cut(s) 1680
PfeI GAWTC 2 cut(s) 283, 619
PflFI GACNNNGTC 1 cut(s) 70
PkrI GCNGC 8 cut(s) 19, 31, 493, 502, 772, 1208, 1494, 1497
PleI GAGTC 2 cut(s) 370, 459
PpsI GAGTC 2 cut(s) 370, 459
Psp6I CCWGG 1 cut(s) 1228
PspFI CCCAGC 1 cut(s) 1626
PspGI CCWGG 1 cut(s) 1228
PspN4I GGNNCC 2 cut(s) 1184, 1623
PstNI CAGNNNCTG 1 cut(s) 1220
PsuI RGATCY 2 cut(s) 543, 643
PsyI GACNNNGTC 1 cut(s) 70
PvuII CAGCTG 1 cut(s) 773
RsaI GTAC 3 cut(s) 523, 599, 611
RsaNI GTAC 3 cut(s) 522, 598, 610
RseI CAYNNNNRTG 2 cut(s) 920, 1649
SapI GCTCTTC 1 cut(s) 1216
SaqAI TTAA 5 cut(s) 149, 348, 650, 870, 1824
SatI GCNGC 8 cut(s) 18, 30, 492, 501, 771, 1207, 1493, 1496
Sau3AI GATC 4 cut(s) 50, 543, 574, 643
SchI GAGTC 2 cut(s) 371, 460
ScrFI CCNGG 3 cut(s) 1230, 1269, 1270
SduI GDGCHC 2 cut(s) 310, 1626
SfaNI GCATC 8 cut(s) 25, 304, 616, 748, 1066, 1149, 1566, 1641
SfuI TTCGAA 2 cut(s) 803, 1487
SmaI CCCGGG 1 cut(s) 1270
SmiMI CAYNNNNRTG 2 cut(s) 920, 1649
SsiI CCGC 5 cut(s) 29, 32, 377, 1292, 1767
SspMI CTAG 3 cut(s) 318, 689, 1197
StyD4I CCNGG 3 cut(s) 1228, 1267, 1268
StyI CCWWGG 1 cut(s) 903
TaaI ACNGT 6 cut(s) 10, 263, 781, 1187, 1554, 1564
TaiI ACGT 1 cut(s) 933
TatI WGTACW 2 cut(s) 597, 609
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 2 cut(s) 283, 619
Tru1I TTAA 5 cut(s) 149, 348, 650, 870, 1824
Tru9I TTAA 5 cut(s) 149, 348, 650, 870, 1824
TscAI CASTG 3 cut(s) 69, 643, 786
TseFI GTSAC 4 cut(s) 64, 103, 367, 1348
TseI GCWGC 7 cut(s) 17, 491, 500, 770, 1206, 1492, 1495
Tsp45I GTSAC 4 cut(s) 64, 103, 367, 1348
TspDTI ATGAA 8 cut(s) 550, 578, 629, 897, 1082, 1314, 1544, 1740
TspGWI ACGGA 1 cut(s) 91
TspMI CCCGGG 1 cut(s) 1268
TspRI CASTG 3 cut(s) 69, 643, 786
Tth111I GACNNNGTC 1 cut(s) 70
XapI RAATTY 4 cut(s) 352, 401, 557, 794
XceI RCATGY 2 cut(s) 29, 500
XmaI CCCGGG 1 cut(s) 1268
XmiI GTMKAC 2 cut(s) 115, 1454
XmnI GAANNNNTTC 1 cut(s) 1680
XspI CTAG 3 cut(s) 318, 689, 1197
Zsp2I ATGCAT 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.