AT2G40660

Putative tRNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0012158
Physical Location & Seq
Forward (+)
16965909 .. 16969119
3211 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G40660.1

Sequence Viewer

Length: 1170 bp
ATGGATTCAAAGGCGAAGCAGATGATTCTTTCAGCTCTCTGCAAACACTTCTCTTTGGATCCTGAACCTTTTGTGGGCGGAGCGGGTGAAAGTGATATCAAGACACTTTATTCTAATGTATTGAAGGCATCTGGAAAGGAAGTATCTGCACAAAACAACGATGTTTTGAAGTGGCTAGATTTTGCGGAAGGTTTCTCTTCAGATTCAAAGGATTGGTTTTCAGCTCTTGAAAAGTTGAATTTAGAGTTAGCTACCAAGTCAGTGCTCTTGGGAAACGGATTGACGCCATCGGCAGCTGATGTTGCTGTGTTTTCAGCTCTGCATTCTTCAGTGCTTGGCCTCTCAGATTCAGACAAGGAGAAAGTACCACATGTCATAAGATGGGTGAATTACATCCAGAATAAAGAAGAGTTGTCGACATTATTTGCACCAATACCTGTGAAGCTACCAGAATTTAGCTTCGAGGTACCAAAACCTGCCATTAAGGTGGAAACAAATTCTAATTCAAAAAAAGCTGCAGAAGGTGTAAAGCCTGTTGACAAGCCAGATGTGCAGCCGCAGCTGGGCACAAAGAAAACTGAGCCAGAGGAACCAAAGAAAAATGCAGCAAAAGAGAAGGATGCCAAAAAGGAAAAGAAGAAGCCTGCTGAACCAGAACCAGCTAAGAAAGAGGCAGAGCTTAGTGTCAGTTTGCTTAACATTCAGGTTGGTCTAATTCGTAAAGCATGGAAGCACCCATCGGCGGATAGTTTGCTGGTGGAAGAAATAGATGTCGGGGAGGATAAAGTGAGACAAGTAGTTAGTGGCTTAGCAAAGTTCTGCAGTCCTGAGGATCTAACGAACCGCCTCGTTGCACTCATCACAAATGTCAAACCTGGAAAGCTAAGAGATGTAATGTCACAAGGACTGGTTCTTTGTGCTTCAAGTGAAGATCACTCGGTGGTAGAGCCGTTGCTACCACCTGCTGGTGCTAAACCAGGAGAGCGTGTTTCATTTTCAGGGATTGAAGGAAAGCCAGAAGATGTACTGAACCCAAAAAAGAAGCAACTAGAGAAGATAACACCGGGTCTCTACACTGATGAAAATGGCGTAGCCACATACAAAGGCATACAATTCATGACCTCGGCTGGACCTTGCACTTCCAGCATCCCCAAGGCGACCATCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C tRNA_bind

Protein Analysis

389

Amino Acids

42.09

Weight (kDa)

6.98

Isoelectric Point (pI)

32.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arc1p_N_like PF21972 75 - 135 1.2e-10 Nuclear-export cofactor Arc1p
tRNA_bind PF01588 234 - 329 4.7e-26 Putative tRNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012158)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40660
fragaria_vesca FvH4_7g25960
malus_domestica MD01G1168000.v1.1 MD07G1234000.v1.1
prunus_persica Prupe.2G264400_v2.0.a1
pyrus_communis pycom01g18100 pycom07g21180
rosa_chinensis RchiOBHm_Chr1g0372341
rosa_laevigata RLG00000026906
rosa_multiflora Rmu_sc0001755.1_g000007
rosa_roxburghii Rroxscaffold_4G00285030
rosa_rugosa Rorug01G0371300 Rorug01G0371300 Rorug01G0371300
rosa_samantha Rh1AG379700 Rh1BG343200 Rh1CG356700 Rh1DG374400
rosa_wichuraiana Rw1G033380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 970
Acc36I ACCTGC 2 cut(s) 484, 970
Acc65I GGTACC 1 cut(s) 466
AccB1I GGYRCC 1 cut(s) 466
AccB7I CCANNNNNTGG 1 cut(s) 965
AccBSI CCGCTC 1 cut(s) 83
AccI GTMKAC 1 cut(s) 416
AciI CCGC 6 cut(s) 78, 83, 185, 557, 743, 844
AclWI GGATC 3 cut(s) 53, 66, 840
AcsI RAATTY 3 cut(s) 238, 452, 496
AcuI CTGAAG 2 cut(s) 183, 312
AcyI GRCGYC 1 cut(s) 284
AdeI CACNNNGTG 1 cut(s) 940
AfaI GTAC 3 cut(s) 366, 468, 1026
AfiI CCNNNNNNNGG 4 cut(s) 74, 563, 742, 965
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 9 cut(s) 9, 124, 169, 207, 230, 238, 507, 924, 1007
AjnI CCWGG 2 cut(s) 874, 976
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 2 cut(s) 784, 1073
AlwI GGATC 3 cut(s) 53, 66, 840
AoxI GGCC 1 cut(s) 337
ApeKI GCWGC 5 cut(s) 293, 515, 553, 559, 605
ApoI RAATTY 3 cut(s) 238, 452, 496
ArsI GACNNNNNNTTYG 2 cut(s) 897, 929
Asp718I GGTACC 1 cut(s) 466
AspS9I GGNCC 1 cut(s) 1130
AsuC2I CCSGG 1 cut(s) 1065
AsuHPI GGTGA 2 cut(s) 98, 397
AvaII GGWCC 1 cut(s) 1130
AxyI CCTNAGG 1 cut(s) 828
BaeGI GKGCMC 1 cut(s) 569
BamHI GGATCC 1 cut(s) 58
BanI GGYRCC 1 cut(s) 466
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 5 cut(s) 305, 502, 565, 571, 617
BccI CCATC 4 cut(s) 295, 375, 745, 1169
BceAI ACGGC 1 cut(s) 934
BciT130I CCWGG 2 cut(s) 876, 978
BcnI CCSGG 1 cut(s) 1065
BcoDI GTCTC 2 cut(s) 784, 1073
BfaI CTAG 2 cut(s) 176, 1049
BfmI CTRYAG 2 cut(s) 516, 820
BfuAI ACCTGC 2 cut(s) 484, 970
BisI GCNGC 6 cut(s) 294, 516, 554, 557, 560, 606
BlpI GCTNAGC 1 cut(s) 808
BlsI GCNGC 6 cut(s) 295, 517, 555, 558, 561, 607
Bme1390I CCNGG 3 cut(s) 876, 978, 1065
Bme18I GGWCC 1 cut(s) 1130
BmgT120I GGNCC 1 cut(s) 1130
BmiI GGNNCC 3 cut(s) 60, 468, 591
BmrFI CCNGG 3 cut(s) 876, 978, 1065
BmsI GCATC 3 cut(s) 137, 610, 1155
Bpu1102I GCTNAGC 1 cut(s) 808
BpuMI CCSGG 1 cut(s) 1065
BsaHI GRCGYC 1 cut(s) 284
BsaI GGTCTC 1 cut(s) 1073
BsaJI CCNNGG 2 cut(s) 1122, 1152
BsaXI ACNNNNNCTCC 2 cut(s) 972, 1002
Bsc4I CCNNNNNNNGG 4 cut(s) 74, 563, 742, 965
Bse1I ACTGG 1 cut(s) 912
Bse21I CCTNAGG 1 cut(s) 828
BseBI CCWGG 2 cut(s) 876, 978
BseDI CCNNGG 2 cut(s) 1122, 1152
BseGI GGATG 3 cut(s) 393, 625, 1146
BseLI CCNNNNNNNGG 4 cut(s) 74, 563, 742, 965
BseMII CTCAG 3 cut(s) 357, 570, 819
BseNI ACTGG 1 cut(s) 912
BseSI GKGCMC 1 cut(s) 569
BseXI GCAGC 5 cut(s) 305, 502, 565, 571, 617
BseYI CCCAGC 1 cut(s) 562
BsgI GTGCAG 2 cut(s) 132, 572
BshFI GGCC 1 cut(s) 339
BshNI GGYRCC 1 cut(s) 466
BsiHKAI GWGCWC 1 cut(s) 267
BsiSI CCGG 1 cut(s) 1064
BslI CCNNNNNNNGG 4 cut(s) 74, 563, 742, 965
BsmAI GTCTC 2 cut(s) 784, 1073
BsmI GAATGC 1 cut(s) 322
BsnI GGCC 1 cut(s) 339
Bso31I GGTCTC 1 cut(s) 1073
Bsp1286I GDGCHC 2 cut(s) 267, 569
Bsp143I GATC 3 cut(s) 58, 832, 931
Bsp1720I GCTNAGC 1 cut(s) 808
BspACI CCGC 6 cut(s) 78, 83, 185, 557, 743, 844
BspANI GGCC 1 cut(s) 339
BspCNI CTCAG 3 cut(s) 356, 571, 820
BspHI TCATGA 1 cut(s) 1116
BspLI GGNNCC 3 cut(s) 60, 468, 591
BspMAI CTGCAG 2 cut(s) 520, 824
BspMI ACCTGC 2 cut(s) 484, 970
BspPI GGATC 3 cut(s) 53, 66, 840
BspT107I GGYRCC 1 cut(s) 466
BspTNI GGTCTC 1 cut(s) 1073
BsrBI CCGCTC 1 cut(s) 83
BsrI ACTGG 1 cut(s) 912
BssECI CCNNGG 2 cut(s) 1122, 1152
BssMI GATC 3 cut(s) 58, 832, 931
BssNI GRCGYC 1 cut(s) 284
BssT1I CCWWGG 1 cut(s) 1152
Bst2UI CCWGG 2 cut(s) 876, 978
Bst6I CTCTTC 2 cut(s) 202, 402
BstACI GRCGYC 1 cut(s) 284
BstC8I GCNNGC 1 cut(s) 645
BstDEI CTNAG 7 cut(s) 343, 579, 663, 680, 808, 828, 884
BstF5I GGATG 3 cut(s) 393, 625, 1146
BstKTI GATC 3 cut(s) 61, 835, 934
BstMAI GTCTC 2 cut(s) 784, 1073
BstMBI GATC 3 cut(s) 58, 832, 931
BstMWI GCNNNNNNNGC 4 cut(s) 302, 550, 559, 1143
BstNI CCWGG 2 cut(s) 876, 978
BstNSI RCATGY 1 cut(s) 374
BstSCI CCNGG 3 cut(s) 874, 976, 1063
BstSFI CTRYAG 2 cut(s) 516, 820
BstSLI GKGCMC 1 cut(s) 569
BstV1I GCAGC 5 cut(s) 305, 502, 565, 571, 617
BstX2I RGATCY 2 cut(s) 58, 832
BstXI CCANNNNNNTGG 1 cut(s) 487
BstYI RGATCY 2 cut(s) 58, 832
Bsu36I CCTNAGG 1 cut(s) 828
BsuRI GGCC 1 cut(s) 339
BtsCI GGATG 3 cut(s) 393, 625, 1146
BtsIMutI CAGTG 3 cut(s) 267, 336, 1074
BveI ACCTGC 2 cut(s) 484, 970
Cac8I GCNNGC 1 cut(s) 645
CciI TCATGA 1 cut(s) 1116
Cfr13I GGNCC 1 cut(s) 1130
CseI GACGC 1 cut(s) 292
Csp6I GTAC 3 cut(s) 365, 467, 1025
CviAII CATG 3 cut(s) 371, 726, 1117
CviQI GTAC 3 cut(s) 365, 467, 1025
DdeI CTNAG 7 cut(s) 343, 579, 663, 680, 808, 828, 884
DpnI GATC 3 cut(s) 60, 834, 933
DpnII GATC 3 cut(s) 58, 832, 931
DraIII CACNNNGTG 1 cut(s) 940
Eam1104I CTCTTC 2 cut(s) 202, 402
EarI CTCTTC 2 cut(s) 202, 402
EciI GGCGGA 2 cut(s) 93, 758
Eco130I CCWWGG 1 cut(s) 1152
Eco31I GGTCTC 1 cut(s) 1073
Eco32I GATATC 1 cut(s) 97
Eco47I GGWCC 1 cut(s) 1130
Eco57I CTGAAG 2 cut(s) 183, 312
Eco81I CCTNAGG 1 cut(s) 828
EcoRII CCWGG 2 cut(s) 874, 976
EcoRV GATATC 1 cut(s) 97
EcoT14I CCWWGG 1 cut(s) 1152
ErhI CCWWGG 1 cut(s) 1152
FaeI CATG 3 cut(s) 374, 729, 1120
FaiI YATR 6 cut(s) 372, 377, 727, 1099, 1109, 1118
FatI CATG 3 cut(s) 370, 725, 1116
FauI CCCGC 1 cut(s) 76
FblI GTMKAC 1 cut(s) 416
Fnu4HI GCNGC 6 cut(s) 294, 516, 554, 557, 560, 606
FokI GGATG 3 cut(s) 380, 632, 1133
Fsp4HI GCNGC 6 cut(s) 294, 516, 554, 557, 560, 606
FspBI CTAG 2 cut(s) 176, 1049
GluI GCNGC 6 cut(s) 294, 516, 554, 557, 560, 606
GsaI CCCAGC 1 cut(s) 566
HaeIII GGCC 1 cut(s) 339
HapII CCGG 1 cut(s) 1064
HgaI GACGC 1 cut(s) 292
Hin1I GRCGYC 1 cut(s) 284
Hin1II CATG 3 cut(s) 374, 729, 1120
HincII GTYRAC 2 cut(s) 417, 538
HindII GTYRAC 2 cut(s) 417, 538
HinfI GANTC 4 cut(s) 5, 25, 203, 347
HpaII CCGG 1 cut(s) 1064
HphI GGTGA 2 cut(s) 98, 397
Hpy166II GTNNAC 2 cut(s) 417, 538
Hpy188I TCNGA 3 cut(s) 202, 346, 352
Hpy188III TCNNGA 7 cut(s) 62, 100, 132, 227, 397, 827, 1117
Hpy8I GTNNAC 2 cut(s) 417, 538
HpyAV CCTTC 5 cut(s) 118, 182, 515, 610, 1001
HpyF10VI GCNNNNNNNGC 4 cut(s) 302, 550, 559, 1143
HpyF3I CTNAG 7 cut(s) 343, 579, 663, 680, 808, 828, 884
Hsp92I GRCGYC 1 cut(s) 284
Hsp92II CATG 3 cut(s) 374, 729, 1120
KpnI GGTACC 1 cut(s) 470
Kzo9I GATC 3 cut(s) 58, 832, 931
LmnI GCTCC 1 cut(s) 80
Lsp1109I GCAGC 5 cut(s) 305, 502, 565, 571, 617
LweI GCATC 3 cut(s) 137, 610, 1155
MaeI CTAG 2 cut(s) 176, 1049
MaeIII GTNAC 1 cut(s) 897
MalI GATC 3 cut(s) 60, 834, 933
MbiI CCGCTC 1 cut(s) 83
MboI GATC 3 cut(s) 58, 832, 931
MboII GAAGA 8 cut(s) 189, 318, 419, 649, 773, 941, 1031, 1066
MflI RGATCY 2 cut(s) 58, 832
MhlI GDGCHC 2 cut(s) 267, 569
MluCI AATT 7 cut(s) 238, 388, 452, 496, 502, 714, 1112
MnlI CCTC 8 cut(s) 350, 457, 580, 664, 772, 823, 857, 1132
MseI TTAA 2 cut(s) 483, 696
MslI CAYNNNNRTG 1 cut(s) 485
MspA1I CMGCKG 2 cut(s) 296, 562
MspI CCGG 1 cut(s) 1064
MspR9I CCNGG 3 cut(s) 876, 978, 1065
Mva1269I GAATGC 1 cut(s) 322
MvaI CCWGG 2 cut(s) 876, 978
MwoI GCNNNNNNNGC 4 cut(s) 302, 550, 559, 1143
NciI CCSGG 1 cut(s) 1065
NdeII GATC 3 cut(s) 58, 832, 931
NlaIII CATG 3 cut(s) 374, 729, 1120
NlaIV GGNNCC 3 cut(s) 60, 468, 591
NmeAIII GCCGAG 1 cut(s) 1103
NmuCI GTSAC 1 cut(s) 897
NspI RCATGY 1 cut(s) 374
PagI TCATGA 1 cut(s) 1116
PaqCI CACCTGC 1 cut(s) 970
PciI ACATGT 1 cut(s) 370
PctI GAATGC 1 cut(s) 322
PfeI GAWTC 4 cut(s) 5, 25, 203, 347
PflMI CCANNNNNTGG 1 cut(s) 965
PkrI GCNGC 6 cut(s) 295, 517, 555, 558, 561, 607
PscI ACATGT 1 cut(s) 370
Psp6I CCWGG 2 cut(s) 874, 976
PspFI CCCAGC 1 cut(s) 562
PspGI CCWGG 2 cut(s) 874, 976
PspN4I GGNNCC 3 cut(s) 60, 468, 591
PspPI GGNCC 1 cut(s) 1130
PstI CTGCAG 2 cut(s) 520, 824
PsuI RGATCY 2 cut(s) 58, 832
PvuII CAGCTG 2 cut(s) 296, 562
RsaI GTAC 3 cut(s) 366, 468, 1026
RsaNI GTAC 3 cut(s) 365, 467, 1025
RseI CAYNNNNRTG 1 cut(s) 485
SalI GTCGAC 1 cut(s) 415
SaqAI TTAA 2 cut(s) 483, 696
SatI GCNGC 6 cut(s) 294, 516, 554, 557, 560, 606
Sau3AI GATC 3 cut(s) 58, 832, 931
Sau96I GGNCC 1 cut(s) 1130
ScrFI CCNGG 3 cut(s) 876, 978, 1065
SduI GDGCHC 2 cut(s) 267, 569
SfaNI GCATC 3 cut(s) 137, 610, 1155
SfcI CTRYAG 2 cut(s) 516, 820
SinI GGWCC 1 cut(s) 1130
SmiMI CAYNNNNRTG 1 cut(s) 485
Sse9I AATT 7 cut(s) 238, 388, 452, 496, 502, 714, 1112
SsiI CCGC 6 cut(s) 78, 83, 185, 557, 743, 844
SspMI CTAG 2 cut(s) 176, 1049
StyD4I CCNGG 3 cut(s) 874, 976, 1063
StyI CCWWGG 1 cut(s) 1152
TaqI TCGA 2 cut(s) 416, 462
TasI AATT 7 cut(s) 238, 388, 452, 496, 502, 714, 1112
TatI WGTACW 1 cut(s) 1024
TauI GCSGC 1 cut(s) 559
TfiI GAWTC 4 cut(s) 5, 25, 203, 347
Tru1I TTAA 2 cut(s) 483, 696
Tru9I TTAA 2 cut(s) 483, 696
TscAI CASTG 3 cut(s) 267, 336, 1081
TseFI GTSAC 1 cut(s) 897
TseI GCWGC 5 cut(s) 293, 515, 553, 559, 605
Tsp45I GTSAC 1 cut(s) 897
TspDTI ATGAA 3 cut(s) 981, 1095, 1105
TspGWI ACGGA 1 cut(s) 291
TspRI CASTG 3 cut(s) 267, 336, 1081
Van91I CCANNNNNTGG 1 cut(s) 965
VpaK11BI GGWCC 1 cut(s) 1130
XapI RAATTY 3 cut(s) 238, 452, 496
XceI RCATGY 1 cut(s) 374
XmiI GTMKAC 1 cut(s) 416
XspI CTAG 2 cut(s) 176, 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.