MD01G1168000.v1.1

Putative tRNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012158
Physical Location & Seq
Forward (+)
27245445 .. 27249900
4456 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1168000.v1.1.491

Sequence Viewer

Length: 1236 bp
ATGGCAGCTAATGCAAGCACCAGCGTAAGCAGAAAGCAGCTGATACTATCGGCTCTCTGCAAATACTTCTCTCTCGATCCTAAATCGTTGCCGGAAAACATCGCTGGCGATGAAGTCACGAGCTTGTACTCAACCGTACTGAAATCAGCCAAAAAGGACGCTGCAGGGGAAGTGAAAGATGGGGTGGTGAATTGGATAAGATATGCAGAGGGTTTTCCTACAGATTCCCAGGCATGCTCTGGAGCTCTCAAAGGGTTGAATGAAACCTTGGTTGATGTGTCAGTGCTTTTGGGCAATGGGTTCACCCCCTCTGAAGCTGATGCTGTTGTGTTTGCTGCAGTGCATTCTTCTGTGATTGGACTTTCAAATTCAGATAGGCAAAAGTTGCCGCATTTGTTGAGATGGGTGGATTACATCCAGAACAAGGTGGATTTTGGGGACATACTTGAGAAGATAGTGATAGAGAAGGATAAATTTGATTTTCGAAAGTTGGATGAATTGATGGGAGCAAAAAATGCAGGTAAGGTAGAAATTGATTCAAATGCAAAGAAGACTGTTCAAGGCGCAAAAAGTGCAGAAAAGCCAGAGGCAGACCTGAACACAAAGAAAAGTGATGCTGGGGTAAAGGCAGCAGGAGATGAGAAAATTACTTCGGATAAGAAAGCGGATAAGAAAGCAGATAAGAAAGCTACTGAAGAGAAGAAAAAATCACCAGAAACAGAGGCAGTTGAGAAAGACAAAGAAGTTAGTGTCAGTTTATTGAATATACAGGTTGGCCTCGTGCGCAAAGCTTGGAAGCATCCGTCTGCCGATAGTTTACTGGTTGAGGAGATAGATGTTGGAGAAGCTAAATTACGGCAAGTGGTCAGCGGCTTGGCAAAATATATCAGTCCAGAGGAGTTGACAAACCGTCATGTTGTGCTGATTACGAATGTGAAACCTGGGAAGTTACGGGATGTGATGTCACAAGGACTGGTGCTATGTGCTTCTAACGAAGATCACACCCTTGTAGAGCCTTTGCTCCCTCCACAAGGGGCTAAACCGGGGGAACGTGTATCATTTTCTGGGATTGATGGAAAGCCAGAAGATGTTCTGAACCCAAAAAAGAAACAGCTGGACAAGATCACTCCGCATCTTTTCACTGACGACAAGGGCGTGGCTACCTTCAAGGGTATTCCGTTTATGACCTCGGCTGGGCCTTGTACATCATCCATCCCCAAGGCAACCATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C tRNA_bind

Protein Analysis

412

Amino Acids

44.35

Weight (kDa)

8.09

Isoelectric Point (pI)

27.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arc1p_N_like PF21972 81 - 142 4.4e-10 Nuclear-export cofactor Arc1p
tRNA_bind PF01588 256 - 351 9.4e-28 Putative tRNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012158)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40660
fragaria_vesca FvH4_7g25960
malus_domestica MD01G1168000.v1.1 MD07G1234000.v1.1
prunus_persica Prupe.2G264400_v2.0.a1
pyrus_communis pycom01g18100 pycom07g21180
rosa_chinensis RchiOBHm_Chr1g0372341
rosa_laevigata RLG00000026906
rosa_multiflora Rmu_sc0001755.1_g000007
rosa_roxburghii Rroxscaffold_4G00285030
rosa_rugosa Rorug01G0371300 Rorug01G0371300 Rorug01G0371300
rosa_samantha Rh1AG379700 Rh1BG343200 Rh1CG356700 Rh1DG374400
rosa_wichuraiana Rw1G033380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 785
Acc36I ACCTGC 1 cut(s) 509
AciI CCGC 4 cut(s) 389, 665, 870, 1130
AclWI GGATC 1 cut(s) 71
AcsI RAATTY 2 cut(s) 367, 473
AcuI CTGAAG 2 cut(s) 333, 714
AfaI GTAC 3 cut(s) 128, 138, 1204
AfiI CCNNNNNNNGG 3 cut(s) 424, 1031, 1194
AflIII ACRYGT 1 cut(s) 1051
AgsI TTSAA 6 cut(s) 259, 366, 540, 560, 763, 1168
AhdI GACNNNNNGTC 1 cut(s) 909
AjnI CCWGG 2 cut(s) 228, 940
AjuI GAANNNNNNNTTGG 2 cut(s) 251, 283
AluBI AGCT 9 cut(s) 8, 40, 123, 245, 317, 689, 791, 848, 1114
AluI AGCT 9 cut(s) 8, 40, 123, 245, 317, 689, 791, 848, 1114
Alw21I GWGCWC 1 cut(s) 247
AlwI GGATC 1 cut(s) 71
AoxI GGCC 2 cut(s) 775, 1196
ApeKI GCWGC 5 cut(s) 5, 37, 161, 335, 629
ApoI RAATTY 2 cut(s) 367, 473
Asp700I GAANNNNTTC 1 cut(s) 1089
AspLEI GCGC 2 cut(s) 566, 786
AspS9I GGNCC 1 cut(s) 1196
AsuC2I CCSGG 1 cut(s) 1044
AsuHPI GGTGA 3 cut(s) 199, 295, 702
AsuII TTCGAA 1 cut(s) 484
BanII GRGCYC 1 cut(s) 247
BarI GAAGNNNNNNTAC 2 cut(s) 837, 869
BauI CACGAG 2 cut(s) 118, 779
BbsI GAAGAC 1 cut(s) 557
Bbv12I GWGCWC 1 cut(s) 247
BbvI GCAGC 5 cut(s) 17, 49, 148, 322, 641
BccI CCATC 6 cut(s) 173, 396, 496, 1067, 1220, 1235
BceAI ACGGC 1 cut(s) 872
BciT130I CCWGG 2 cut(s) 230, 942
BcnI CCSGG 1 cut(s) 1044
BfmI CTRYAG 3 cut(s) 162, 219, 336
BfuAI ACCTGC 1 cut(s) 509
BisI GCNGC 7 cut(s) 6, 38, 162, 336, 389, 630, 871
BlsI GCNGC 7 cut(s) 7, 39, 163, 337, 390, 631, 872
Bme1390I CCNGG 3 cut(s) 230, 942, 1044
BmeRI GACNNNNNGTC 1 cut(s) 909
BmgT120I GGNCC 1 cut(s) 1196
BmrFI CCNGG 3 cut(s) 230, 942, 1044
BmsI GCATC 4 cut(s) 310, 604, 808, 1141
BpiI GAAGAC 1 cut(s) 557
BpmI CTGGAG 1 cut(s) 261
Bpu14I TTCGAA 1 cut(s) 484
BpuEI CTTGAG 1 cut(s) 467
BpuMI CCSGG 1 cut(s) 1044
BsaJI CCNNGG 6 cut(s) 228, 267, 941, 1043, 1188, 1218
Bsc4I CCNNNNNNNGG 3 cut(s) 424, 1031, 1194
Bse1I ACTGG 2 cut(s) 825, 978
Bse3DI GCAATG 1 cut(s) 301
BseBI CCWGG 2 cut(s) 230, 942
BseDI CCNNGG 6 cut(s) 228, 267, 941, 1043, 1188, 1218
BseGI GGATG 6 cut(s) 414, 499, 799, 961, 1208, 1212
BseLI CCNNNNNNNGG 3 cut(s) 424, 1031, 1194
BseMI GCAATG 1 cut(s) 301
BseNI ACTGG 2 cut(s) 825, 978
BseRI GAGGAG 2 cut(s) 842, 911
BseXI GCAGC 5 cut(s) 17, 49, 148, 322, 641
BseYI CCCAGC 2 cut(s) 617, 1193
BsgI GTGCAG 1 cut(s) 594
BshFI GGCC 2 cut(s) 777, 1198
BsiHKAI GWGCWC 1 cut(s) 247
BsiSI CCGG 2 cut(s) 92, 1043
BslFI GGGAC 1 cut(s) 452
BslI CCNNNNNNNGG 3 cut(s) 424, 1031, 1194
BsmFI GGGAC 1 cut(s) 452
BsmI GAATGC 1 cut(s) 343
BsnI GGCC 2 cut(s) 777, 1198
Bsp119I TTCGAA 1 cut(s) 484
Bsp1286I GDGCHC 1 cut(s) 247
Bsp1407I TGTACA 1 cut(s) 1202
Bsp143I GATC 3 cut(s) 76, 997, 1122
BspACI CCGC 4 cut(s) 389, 665, 870, 1130
BspANI GGCC 2 cut(s) 777, 1198
BspMAI CTGCAG 2 cut(s) 166, 340
BspMI ACCTGC 1 cut(s) 509
BspPI GGATC 1 cut(s) 71
BspT104I TTCGAA 1 cut(s) 484
BsrDI GCAATG 1 cut(s) 301
BsrGI TGTACA 1 cut(s) 1202
BsrI ACTGG 2 cut(s) 825, 978
BssECI CCNNGG 6 cut(s) 228, 267, 941, 1043, 1188, 1218
BssMI GATC 3 cut(s) 76, 997, 1122
BssSI CACGAG 2 cut(s) 118, 779
BssT1I CCWWGG 2 cut(s) 267, 1218
Bst2BI CACGAG 2 cut(s) 118, 779
Bst2UI CCWGG 2 cut(s) 230, 942
Bst4CI ACNGT 3 cut(s) 136, 556, 911
Bst6I CTCTTC 1 cut(s) 690
BstAPI GCANNNNNTGC 4 cut(s) 11, 385, 515, 572
BstAUI TGTACA 1 cut(s) 1202
BstBI TTCGAA 1 cut(s) 484
BstC8I GCNNGC 3 cut(s) 16, 106, 235
BstENI CCTNNNNNAGG 1 cut(s) 1029
BstF5I GGATG 6 cut(s) 414, 499, 799, 961, 1208, 1212
BstHHI GCGC 2 cut(s) 566, 786
BstKTI GATC 3 cut(s) 79, 1000, 1125
BstMBI GATC 3 cut(s) 76, 997, 1122
BstMWI GCNNNNNNNGC 5 cut(s) 11, 385, 515, 572, 783
BstNI CCWGG 2 cut(s) 230, 942
BstNSI RCATGY 1 cut(s) 237
BstSCI CCNGG 3 cut(s) 228, 940, 1042
BstSFI CTRYAG 3 cut(s) 162, 219, 336
BstV1I GCAGC 5 cut(s) 17, 49, 148, 322, 641
BstV2I GAAGAC 1 cut(s) 557
BsuRI GGCC 2 cut(s) 777, 1198
BtgZI GCGATG 2 cut(s) 85, 123
BtsCI GGATG 6 cut(s) 414, 499, 799, 961, 1208, 1212
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 3 cut(s) 288, 345, 1140
BveI ACCTGC 1 cut(s) 509
Cac8I GCNNGC 3 cut(s) 16, 106, 235
CfoI GCGC 2 cut(s) 566, 786
Cfr13I GGNCC 1 cut(s) 1196
CseI GACGC 1 cut(s) 167
Csp6I GTAC 3 cut(s) 127, 137, 1203
CviAII CATG 2 cut(s) 234, 914
CviQI GTAC 3 cut(s) 127, 137, 1203
DpnI GATC 3 cut(s) 78, 999, 1124
DpnII GATC 3 cut(s) 76, 997, 1122
DriI GACNNNNNGTC 1 cut(s) 909
Eam1104I CTCTTC 1 cut(s) 690
Eam1105I GACNNNNNGTC 1 cut(s) 909
EarI CTCTTC 1 cut(s) 690
Ecl136II GAGCTC 1 cut(s) 245
Eco130I CCWWGG 2 cut(s) 267, 1218
Eco24I GRGCYC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 245
Eco57I CTGAAG 2 cut(s) 333, 714
EcoICRI GAGCTC 1 cut(s) 245
EcoNI CCTNNNNNAGG 1 cut(s) 1029
EcoRII CCWGG 2 cut(s) 228, 940
EcoT14I CCWWGG 2 cut(s) 267, 1218
EcoT38I GRGCYC 1 cut(s) 247
ErhI CCWWGG 2 cut(s) 267, 1218
FaeI CATG 2 cut(s) 237, 917
FaiI YATR 8 cut(s) 204, 235, 443, 767, 885, 915, 982, 1184
FaqI GGGAC 1 cut(s) 452
FatI CATG 2 cut(s) 233, 913
Fnu4HI GCNGC 7 cut(s) 6, 38, 162, 336, 389, 630, 871
FokI GGATG 6 cut(s) 401, 506, 786, 968, 1195, 1199
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 7 cut(s) 6, 38, 162, 336, 389, 630, 871
FspI TGCGCA 1 cut(s) 785
GlaI GCGC 2 cut(s) 565, 785
GluI GCNGC 7 cut(s) 6, 38, 162, 336, 389, 630, 871
GsaI CCCAGC 2 cut(s) 621, 1197
GsuI CTGGAG 1 cut(s) 261
HaeIII GGCC 2 cut(s) 777, 1198
HapII CCGG 2 cut(s) 92, 1043
HgaI GACGC 1 cut(s) 167
HhaI GCGC 2 cut(s) 566, 786
Hin1II CATG 2 cut(s) 237, 917
Hin6I GCGC 2 cut(s) 564, 784
HinP1I GCGC 2 cut(s) 564, 784
HincII GTYRAC 1 cut(s) 903
HindII GTYRAC 1 cut(s) 903
HindIII AAGCTT 1 cut(s) 789
HinfI GANTC 2 cut(s) 224, 536
HpaII CCGG 2 cut(s) 92, 1043
HphI GGTGA 3 cut(s) 199, 295, 702
Hpy166II GTNNAC 3 cut(s) 303, 818, 903
Hpy188I TCNGA 4 cut(s) 313, 373, 655, 1095
Hpy188III TCNNGA 5 cut(s) 74, 118, 240, 418, 893
Hpy8I GTNNAC 3 cut(s) 303, 818, 903
HpyAV CCTTC 2 cut(s) 460, 1174
HpyCH4III ACNGT 3 cut(s) 136, 556, 911
HpyCH4IV ACGT 1 cut(s) 1051
HpyCH4V TGCA 9 cut(s) 14, 60, 164, 206, 338, 343, 518, 545, 575
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 385, 515, 572, 783
HpySE526I ACGT 1 cut(s) 1051
Hsp92II CATG 2 cut(s) 237, 917
HspAI GCGC 2 cut(s) 564, 784
Kzo9I GATC 3 cut(s) 76, 997, 1122
LmnI GCTCC 3 cut(s) 242, 506, 1026
Lsp1109I GCAGC 5 cut(s) 17, 49, 148, 322, 641
LweI GCATC 4 cut(s) 310, 604, 808, 1141
MaeII ACGT 1 cut(s) 1051
MaeIII GTNAC 3 cut(s) 115, 948, 963
MalI GATC 3 cut(s) 78, 999, 1124
MboI GATC 3 cut(s) 76, 997, 1122
MboII GAAGA 7 cut(s) 339, 463, 562, 707, 712, 1007, 1097
MhlI GDGCHC 1 cut(s) 247
MluCI AATT 7 cut(s) 190, 367, 473, 497, 531, 645, 851
MmeI TCCRAC 2 cut(s) 471, 820
MnlI CCTC 9 cut(s) 202, 319, 580, 715, 788, 820, 889, 1035, 1198
MroXI GAANNNNTTC 1 cut(s) 1089
MspA1I CMGCKG 3 cut(s) 40, 870, 1114
MspI CCGG 2 cut(s) 92, 1043
MspR9I CCNGG 3 cut(s) 230, 942, 1044
Mva1269I GAATGC 1 cut(s) 343
MvaI CCWGG 2 cut(s) 230, 942
MwoI GCNNNNNNNGC 5 cut(s) 11, 385, 515, 572, 783
NciI CCSGG 1 cut(s) 1044
NdeII GATC 3 cut(s) 76, 997, 1122
NlaIII CATG 2 cut(s) 237, 917
NmeAIII GCCGAG 1 cut(s) 1169
NmuCI GTSAC 2 cut(s) 115, 963
NsbI TGCGCA 1 cut(s) 785
NspI RCATGY 1 cut(s) 237
NspV TTCGAA 1 cut(s) 484
PaeI GCATGC 1 cut(s) 237
PcsI WCGNNNNNNNCGW 1 cut(s) 1152
PctI GAATGC 1 cut(s) 343
PdmI GAANNNNTTC 1 cut(s) 1089
PfeI GAWTC 2 cut(s) 224, 536
PkrI GCNGC 7 cut(s) 7, 39, 163, 337, 390, 631, 872
Psp124BI GAGCTC 1 cut(s) 247
Psp6I CCWGG 2 cut(s) 228, 940
PspFI CCCAGC 2 cut(s) 617, 1193
PspGI CCWGG 2 cut(s) 228, 940
PspPI GGNCC 1 cut(s) 1196
PstI CTGCAG 2 cut(s) 166, 340
PvuII CAGCTG 2 cut(s) 40, 1114
RsaI GTAC 3 cut(s) 128, 138, 1204
RsaNI GTAC 3 cut(s) 127, 137, 1203
SacI GAGCTC 1 cut(s) 247
SatI GCNGC 7 cut(s) 6, 38, 162, 336, 389, 630, 871
Sau3AI GATC 3 cut(s) 76, 997, 1122
Sau96I GGNCC 1 cut(s) 1196
ScrFI CCNGG 3 cut(s) 230, 942, 1044
SduI GDGCHC 1 cut(s) 247
SfaNI GCATC 4 cut(s) 310, 604, 808, 1141
SfcI CTRYAG 3 cut(s) 162, 219, 336
SfuI TTCGAA 1 cut(s) 484
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
SphI GCATGC 1 cut(s) 237
Sse9I AATT 7 cut(s) 190, 367, 473, 497, 531, 645, 851
SsiI CCGC 4 cut(s) 389, 665, 870, 1130
SstI GAGCTC 1 cut(s) 247
StyD4I CCNGG 3 cut(s) 228, 940, 1042
StyI CCWWGG 2 cut(s) 267, 1218
TaaI ACNGT 3 cut(s) 136, 556, 911
TaiI ACGT 1 cut(s) 1054
TaqI TCGA 2 cut(s) 75, 484
TasI AATT 7 cut(s) 190, 367, 473, 497, 531, 645, 851
TatI WGTACW 2 cut(s) 126, 1202
TauI GCSGC 2 cut(s) 391, 873
TfiI GAWTC 2 cut(s) 224, 536
TscAI CASTG 3 cut(s) 288, 345, 1147
TseFI GTSAC 2 cut(s) 115, 963
TseI GCWGC 5 cut(s) 5, 37, 161, 335, 629
Tsp45I GTSAC 2 cut(s) 115, 963
TspDTI ATGAA 3 cut(s) 126, 276, 510
TspGWI ACGGA 2 cut(s) 792, 1167
TspRI CASTG 3 cut(s) 288, 345, 1147
XagI CCTNNNNNAGG 1 cut(s) 1029
XapI RAATTY 2 cut(s) 367, 473
XceI RCATGY 1 cut(s) 237
XcmI CCANNNNNNNNNTGG 1 cut(s) 236
XmnI GAANNNNTTC 1 cut(s) 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.