Prupe.2G264400_v2.0.a1

Putative tRNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0012158
Physical Location & Seq
Forward (+)
27192829 .. 27197340
4512 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G264400.1

Sequence Viewer

Length: 1206 bp
ATGGCAGCTAATGGAAGCACCAGCCTAGACAGAAAGCGATTGATAGCTTCAGCTCTATGCAAACACTTCTATTTAGATCCTAAAACGTTTCCGGAAAACATTGCTGAAGATGAAATCACGAGTTTCTATACAACCATCTTGAAGTCAGCCAAAAACGACGCCTCAGTGGAAGTGCAAGATGGGGTGCTTCAATGGATAAGATATGCAGACGGTTTTCCTACAGATCCCCAGGCATTCTTTGAAGTTCTCAAAGGGTTAAATGATACCTTGGCTGACGTGTCTGTGCTTTTGGGAAATGGTTTCACACCCTCTGAAGCTGATGTTATTGTGTTCGCTGTGGTGCATTCTTCTGTGATTGGCCTTTCGAATTCAGATAGGCAAAAGTTGCCGCATCTATTGAGATGGGTGGATCACATCCAGAACAAGGTGGATTTCGGGGACTTACTTAAGAAGATAGTGATAGAGAAGGTTCCATTTGACTTTCTAAAGTTGTATGCATTGCGGGGAGCAAAAAGTGCAGGTAAGGTGGAAATAGATTCAAATGCAAAGAAGACTGTTCAAAGTGCAAAAAGCGCAGACAAGCCAGAGGCGGACCTGAACACAAAGAAAAGTGATGCTGAGATAAAGGCTACAGGAGATAAGAAAACAGATAAGAAAGCTACTCAAGAGAAGAAAAAATCTCCAGAAACAGAGGCAGTTGAGAAAGACAAAGAACTTAGTGTCAGTTTGTTGAATATACAGGTTGGCCTCATTCGCAAAGCTTGGAAGCATCCGTCTGCTGATAGTTTACTGGTTGAGGAGATTGATGTTGGAGATGCTAAATTGCGACAAGTGGTCAGTGGCCTGGCAAAATATTGCAGTCCTGAGGAGTTGACGAACCGTAAGGTTGTGCTGATTACAAATGTGAAACCTGGAAAGTTACGCGATGTGGCGTCAGAAGGACTGGTGCTATGTGCTTCTAATGAAGATCACACCCGTGTAGAGCCTTTGCTCCCTCCAGAAGGAGCCAGACCTGGGGAACGTGTGTCATTTTCTGGGGTTGATGGAAAGCCAGAAGATGTCCTCAACCCAAAAAAGAAACAGTTGGAGAAGATCACACCGCATCTTTTCACTGATGATAAGGGTGTGGCTACGTTCAAGGGTATTCCTTTTATGACCTCAGCCGGGCCTTGTACATCATCCATTCCCAAGGCAAGCATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C tRNA_bind

Protein Analysis

402

Amino Acids

43.82

Weight (kDa)

8.12

Isoelectric Point (pI)

31.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012158)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40660
fragaria_vesca FvH4_7g25960
malus_domestica MD01G1168000.v1.1 MD07G1234000.v1.1
prunus_persica Prupe.2G264400_v2.0.a1
pyrus_communis pycom01g18100 pycom07g21180
rosa_chinensis RchiOBHm_Chr1g0372341
rosa_laevigata RLG00000026906
rosa_multiflora Rmu_sc0001755.1_g000007
rosa_roxburghii Rroxscaffold_4G00285030
rosa_rugosa Rorug01G0371300 Rorug01G0371300 Rorug01G0371300
rosa_samantha Rh1AG379700 Rh1BG343200 Rh1CG356700 Rh1DG374400
rosa_wichuraiana Rw1G033380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 509
AccII CGCG 1 cut(s) 924
AccIII TCCGGA 1 cut(s) 91
AciI CCGC 4 cut(s) 389, 502, 590, 1100
AclI AACGTT 1 cut(s) 86
AclWI GGATC 3 cut(s) 71, 218, 417
AcsI RAATTY 1 cut(s) 367
AcuI CTGAAG 3 cut(s) 33, 126, 333
AcyI GRCGYC 2 cut(s) 159, 932
AfaI GTAC 1 cut(s) 1174
AfiI CCNNNNNNNGG 4 cut(s) 424, 1001, 1014, 1164
AflII CTTAAG 1 cut(s) 446
AflIII ACRYGT 2 cut(s) 276, 1021
AgsI TTSAA 7 cut(s) 142, 191, 242, 540, 560, 733, 1138
AhdI GACNNNNNGTC 1 cut(s) 833
AjiI CACGTC 1 cut(s) 277
AjnI CCWGG 4 cut(s) 228, 843, 910, 1012
AleI CACNNNNGTG 1 cut(s) 975
AluBI AGCT 6 cut(s) 8, 47, 53, 317, 659, 761
AluI AGCT 6 cut(s) 8, 47, 53, 317, 659, 761
AlwI GGATC 3 cut(s) 71, 218, 417
Aor13HI TCCGGA 1 cut(s) 91
AoxI GGCC 4 cut(s) 358, 745, 841, 1166
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 367
AspLEI GCGC 1 cut(s) 575
AspS9I GGNCC 2 cut(s) 592, 1166
AsuC2I CCSGG 1 cut(s) 1165
AsuII TTCGAA 1 cut(s) 365
AvaII GGWCC 1 cut(s) 592
AxyI CCTNAGG 1 cut(s) 864
BauI CACGAG 1 cut(s) 118
BbsI GAAGAC 1 cut(s) 557
BbvCI CCTCAGC 1 cut(s) 1159
BbvI GCAGC 1 cut(s) 17
BccI CCATC 4 cut(s) 143, 173, 396, 1037
BciT130I CCWGG 4 cut(s) 230, 845, 912, 1014
BcnI CCSGG 1 cut(s) 1165
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 2 cut(s) 219, 630
BfrI CTTAAG 1 cut(s) 446
BfuAI ACCTGC 1 cut(s) 509
BisI GCNGC 2 cut(s) 6, 389
BlsI GCNGC 2 cut(s) 7, 390
Bme1390I CCNGG 5 cut(s) 230, 845, 912, 1014, 1165
Bme18I GGWCC 1 cut(s) 592
BmeRI GACNNNNNGTC 1 cut(s) 833
BmgBI CACGTC 1 cut(s) 277
BmgT120I GGNCC 2 cut(s) 592, 1166
BmiI GGNNCC 2 cut(s) 471, 1006
BmrFI CCNGG 5 cut(s) 230, 845, 912, 1014, 1165
BmsI GCATC 5 cut(s) 400, 604, 778, 805, 1111
BpiI GAAGAC 1 cut(s) 557
BpmI CTGGAG 2 cut(s) 666, 981
Bpu10I CCTNAGC 1 cut(s) 1159
Bpu14I TTCGAA 1 cut(s) 365
BpuEI CTTGAG 1 cut(s) 648
BpuMI CCSGG 1 cut(s) 1165
BsaHI GRCGYC 2 cut(s) 159, 932
BsaJI CCNNGG 4 cut(s) 228, 267, 1013, 1188
BsaWI WCCGGW 1 cut(s) 91
Bsc4I CCNNNNNNNGG 4 cut(s) 424, 1001, 1014, 1164
Bse1I ACTGG 2 cut(s) 795, 948
Bse21I CCTNAGG 1 cut(s) 864
Bse3DI GCAATG 2 cut(s) 99, 497
BseAI TCCGGA 1 cut(s) 91
BseBI CCWGG 4 cut(s) 230, 845, 912, 1014
BseDI CCNNGG 4 cut(s) 228, 267, 1013, 1188
BseGI GGATG 3 cut(s) 414, 769, 1178
BseLI CCNNNNNNNGG 4 cut(s) 424, 1001, 1014, 1164
BseMI GCAATG 2 cut(s) 99, 497
BseMII CTCAG 4 cut(s) 177, 609, 855, 1173
BseNI ACTGG 2 cut(s) 795, 948
BseRI GAGGAG 2 cut(s) 812, 881
BseXI GCAGC 1 cut(s) 17
BsgI GTGCAG 1 cut(s) 537
Bsh1236I CGCG 1 cut(s) 924
BshFI GGCC 4 cut(s) 360, 747, 843, 1168
BsiSI CCGG 2 cut(s) 92, 1164
BslFI GGGAC 1 cut(s) 452
BslI CCNNNNNNNGG 4 cut(s) 424, 1001, 1014, 1164
BsmFI GGGAC 1 cut(s) 452
BsmI GAATGC 2 cut(s) 233, 343
BsnI GGCC 4 cut(s) 360, 747, 843, 1168
Bsp119I TTCGAA 1 cut(s) 365
Bsp13I TCCGGA 1 cut(s) 91
Bsp1407I TGTACA 1 cut(s) 1172
Bsp143I GATC 5 cut(s) 76, 223, 409, 967, 1092
BspACI CCGC 4 cut(s) 389, 502, 590, 1100
BspANI GGCC 4 cut(s) 360, 747, 843, 1168
BspCNI CTCAG 4 cut(s) 176, 610, 856, 1172
BspEI TCCGGA 1 cut(s) 91
BspFNI CGCG 1 cut(s) 924
BspLI GGNNCC 2 cut(s) 471, 1006
BspMI ACCTGC 1 cut(s) 509
BspPI GGATC 3 cut(s) 71, 218, 417
BspT104I TTCGAA 1 cut(s) 365
BspTI CTTAAG 1 cut(s) 446
BsrDI GCAATG 2 cut(s) 99, 497
BsrGI TGTACA 1 cut(s) 1172
BsrI ACTGG 2 cut(s) 795, 948
BssECI CCNNGG 4 cut(s) 228, 267, 1013, 1188
BssMI GATC 5 cut(s) 76, 223, 409, 967, 1092
BssNI GRCGYC 2 cut(s) 159, 932
BssSI CACGAG 1 cut(s) 118
BssT1I CCWWGG 2 cut(s) 267, 1188
Bst2BI CACGAG 1 cut(s) 118
Bst2UI CCWGG 4 cut(s) 230, 845, 912, 1014
Bst4CI ACNGT 4 cut(s) 212, 556, 881, 1083
BstACI GRCGYC 2 cut(s) 159, 932
BstAFI CTTAAG 1 cut(s) 446
BstAPI GCANNNNNTGC 2 cut(s) 385, 515
BstAUI TGTACA 1 cut(s) 1172
BstBI TTCGAA 1 cut(s) 365
BstC8I GCNNGC 1 cut(s) 1195
BstDEI CTNAG 5 cut(s) 163, 618, 716, 864, 1159
BstENI CCTNNNNNAGG 1 cut(s) 999
BstF5I GGATG 3 cut(s) 414, 769, 1178
BstFNI CGCG 1 cut(s) 924
BstHHI GCGC 1 cut(s) 575
BstKTI GATC 5 cut(s) 79, 226, 412, 970, 1095
BstMBI GATC 5 cut(s) 76, 223, 409, 967, 1092
BstMWI GCNNNNNNNGC 4 cut(s) 385, 515, 572, 753
BstNI CCWGG 4 cut(s) 230, 845, 912, 1014
BstSCI CCNGG 5 cut(s) 228, 843, 910, 1012, 1163
BstSFI CTRYAG 2 cut(s) 219, 630
BstUI CGCG 1 cut(s) 924
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 557
BstX2I RGATCY 2 cut(s) 76, 223
BstYI RGATCY 2 cut(s) 76, 223
Bsu36I CCTNAGG 1 cut(s) 864
BsuRI GGCC 4 cut(s) 360, 747, 843, 1168
BtgZI GCGATG 1 cut(s) 939
BtrI CACGTC 1 cut(s) 277
BtsCI GGATG 3 cut(s) 414, 769, 1178
BtsIMutI CAGTG 3 cut(s) 171, 844, 1110
BveI ACCTGC 1 cut(s) 509
Cac8I GCNNGC 1 cut(s) 1195
CfoI GCGC 1 cut(s) 575
Cfr13I GGNCC 2 cut(s) 592, 1166
CseI GACGC 2 cut(s) 167, 921
Csp6I GTAC 1 cut(s) 1173
CviQI GTAC 1 cut(s) 1173
DdeI CTNAG 5 cut(s) 163, 618, 716, 864, 1159
DpnI GATC 5 cut(s) 78, 225, 411, 969, 1094
DpnII GATC 5 cut(s) 76, 223, 409, 967, 1092
DriI GACNNNNNGTC 1 cut(s) 833
Eam1105I GACNNNNNGTC 1 cut(s) 833
EciI GGCGGA 1 cut(s) 605
Eco130I CCWWGG 2 cut(s) 267, 1188
Eco47I GGWCC 1 cut(s) 592
Eco57I CTGAAG 3 cut(s) 33, 126, 333
Eco81I CCTNAGG 1 cut(s) 864
EcoNI CCTNNNNNAGG 1 cut(s) 999
EcoRI GAATTC 1 cut(s) 367
EcoRII CCWGG 4 cut(s) 228, 843, 910, 1012
EcoT14I CCWWGG 2 cut(s) 267, 1188
EcoT22I ATGCAT 1 cut(s) 499
ErhI CCWWGG 2 cut(s) 267, 1188
FaiI YATR 7 cut(s) 58, 129, 204, 495, 737, 952, 1154
FaqI GGGAC 1 cut(s) 452
FauI CCCGC 1 cut(s) 495
Fnu4HI GCNGC 2 cut(s) 6, 389
FokI GGATG 3 cut(s) 401, 756, 1165
Fsp4HI GCNGC 2 cut(s) 6, 389
FspBI CTAG 1 cut(s) 26
GlaI GCGC 1 cut(s) 574
GluI GCNGC 2 cut(s) 6, 389
GsuI CTGGAG 2 cut(s) 666, 981
HaeIII GGCC 4 cut(s) 360, 747, 843, 1168
HapII CCGG 2 cut(s) 92, 1164
HgaI GACGC 2 cut(s) 167, 921
HhaI GCGC 1 cut(s) 575
Hin1I GRCGYC 2 cut(s) 159, 932
Hin6I GCGC 1 cut(s) 573
HinP1I GCGC 1 cut(s) 573
HincII GTYRAC 1 cut(s) 873
HindII GTYRAC 1 cut(s) 873
HindIII AAGCTT 1 cut(s) 759
HinfI GANTC 1 cut(s) 536
HpaII CCGG 2 cut(s) 92, 1164
Hpy166II GTNNAC 2 cut(s) 788, 873
Hpy188I TCNGA 3 cut(s) 313, 373, 937
Hpy188III TCNNGA 8 cut(s) 92, 118, 139, 418, 665, 683, 863, 998
Hpy8I GTNNAC 2 cut(s) 788, 873
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 3 cut(s) 460, 932, 995
HpyCH4III ACNGT 4 cut(s) 212, 556, 881, 1083
HpyCH4IV ACGT 4 cut(s) 86, 276, 1021, 1133
HpyCH4V TGCA 9 cut(s) 60, 175, 206, 343, 497, 518, 545, 566, 858
HpyF10VI GCNNNNNNNGC 4 cut(s) 385, 515, 572, 753
HpyF3I CTNAG 5 cut(s) 163, 618, 716, 864, 1159
HpySE526I ACGT 4 cut(s) 86, 276, 1021, 1133
Hsp92I GRCGYC 2 cut(s) 159, 932
HspAI GCGC 1 cut(s) 573
Kpn2I TCCGGA 1 cut(s) 91
Kzo9I GATC 5 cut(s) 76, 223, 409, 967, 1092
LmnI GCTCC 3 cut(s) 506, 996, 1004
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 5 cut(s) 400, 604, 778, 805, 1111
MaeI CTAG 1 cut(s) 26
MaeII ACGT 4 cut(s) 86, 276, 1021, 1133
MaeIII GTNAC 1 cut(s) 918
MalI GATC 5 cut(s) 78, 225, 411, 969, 1094
MboI GATC 5 cut(s) 76, 223, 409, 967, 1092
MboII GAAGA 8 cut(s) 119, 339, 463, 562, 682, 977, 1067, 1102
MflI RGATCY 2 cut(s) 76, 223
MluCI AATT 2 cut(s) 367, 821
MmeI TCCRAC 2 cut(s) 790, 1065
Mph1103I ATGCAT 1 cut(s) 499
MroI TCCGGA 1 cut(s) 91
MseI TTAA 2 cut(s) 257, 447
MslI CAYNNNNRTG 1 cut(s) 975
MspCI CTTAAG 1 cut(s) 446
MspI CCGG 2 cut(s) 92, 1164
MspR9I CCNGG 5 cut(s) 230, 845, 912, 1014, 1165
Mva1269I GAATGC 2 cut(s) 233, 343
MvaI CCWGG 4 cut(s) 230, 845, 912, 1014
MvnI CGCG 1 cut(s) 924
MwoI GCNNNNNNNGC 4 cut(s) 385, 515, 572, 753
NciI CCSGG 1 cut(s) 1165
NdeII GATC 5 cut(s) 76, 223, 409, 967, 1092
NlaIV GGNNCC 2 cut(s) 471, 1006
NsiI ATGCAT 1 cut(s) 499
NspV TTCGAA 1 cut(s) 365
OliI CACNNNNGTG 1 cut(s) 975
PctI GAATGC 2 cut(s) 233, 343
PfeI GAWTC 1 cut(s) 536
PkrI GCNGC 2 cut(s) 7, 390
Psp1406I AACGTT 1 cut(s) 86
Psp6I CCWGG 4 cut(s) 228, 843, 910, 1012
PspGI CCWGG 4 cut(s) 228, 843, 910, 1012
PspN4I GGNNCC 2 cut(s) 471, 1006
PspPI GGNCC 2 cut(s) 592, 1166
PsuI RGATCY 2 cut(s) 76, 223
RsaI GTAC 1 cut(s) 1174
RsaNI GTAC 1 cut(s) 1173
RseI CAYNNNNRTG 1 cut(s) 975
SaqAI TTAA 2 cut(s) 257, 447
SatI GCNGC 2 cut(s) 6, 389
Sau3AI GATC 5 cut(s) 76, 223, 409, 967, 1092
Sau96I GGNCC 2 cut(s) 592, 1166
ScrFI CCNGG 5 cut(s) 230, 845, 912, 1014, 1165
SfaNI GCATC 5 cut(s) 400, 604, 778, 805, 1111
SfcI CTRYAG 2 cut(s) 219, 630
SfuI TTCGAA 1 cut(s) 365
SinI GGWCC 1 cut(s) 592
SmiMI CAYNNNNRTG 1 cut(s) 975
SmlI CTYRAG 2 cut(s) 446, 663
SmoI CTYRAG 2 cut(s) 446, 663
Sse9I AATT 2 cut(s) 367, 821
SsiI CCGC 4 cut(s) 389, 502, 590, 1100
SspI AATATT 1 cut(s) 854
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 5 cut(s) 228, 843, 910, 1012, 1163
StyI CCWWGG 2 cut(s) 267, 1188
TaaI ACNGT 4 cut(s) 212, 556, 881, 1083
TaiI ACGT 4 cut(s) 89, 279, 1024, 1136
TaqI TCGA 1 cut(s) 365
TasI AATT 2 cut(s) 367, 821
TatI WGTACW 1 cut(s) 1172
TauI GCSGC 1 cut(s) 391
TfiI GAWTC 1 cut(s) 536
Tru1I TTAA 2 cut(s) 257, 447
Tru9I TTAA 2 cut(s) 257, 447
TscAI CASTG 3 cut(s) 171, 844, 1117
TseI GCWGC 1 cut(s) 5
TspDTI ATGAA 2 cut(s) 126, 978
TspGWI ACGGA 1 cut(s) 762
TspRI CASTG 3 cut(s) 171, 844, 1117
Vha464I CTTAAG 1 cut(s) 446
VpaK11BI GGWCC 1 cut(s) 592
XagI CCTNNNNNAGG 1 cut(s) 999
XapI RAATTY 1 cut(s) 367
XspI CTAG 1 cut(s) 26
Zsp2I ATGCAT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.