MD07G1234000.v1.1

Putative tRNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012158
Physical Location & Seq
Forward (+)
30756197 .. 30760844
4648 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1234000.v1.1.491

Sequence Viewer

Length: 1224 bp
ATGGCAGCTAAGGGAAGTACCAGCGTAAGCAGAAAGCAATTGATTCTATCAGCTCTCTGCAAACACTTCTCTCTCGATCCTAAATCGTTGCCGGAAAACATCGCTGGCGATGAAGTCACGAGCTTGTACTCAACGGTACTGAAATCAGCCAAACACGGCACCTCAGCGCAAGTGAACGATGGGGTGGTGAATTGGGTGAGATATGCAGAGGGTTTTCCTACAGATTCCCAGGCATGCTCTGCAGCTCTCGAAGGGTTGAATGAAACCTTGGCTGACGTTTCAGTGCTTTTGGGCAATGGGTTCACACCCTCTGAAGCTGATGCTGTTGTGTTTTCTGCGGTGCATTCTTCTGTGATTGGTCTTTCAAATACAGATAGGCAAAAGTTGCCGCATTTGTTGAGATGGGTGGATTACATCCAGAACAAGGTAGATTTCGGGGACGTACCTGAGAAGATAGCGGTAGAGAAGGATAAATTTGATTTTCGAAAGTTGGATGAATTGCTGGGAGCAAAAAGTGCAGGTAAGGTGGAAATTGATTCAAATGCAAAGAAGACTGTCCAAAGCACAAAAACCGCAGACAAACTAGGGGCAGACCTGAACACAAAGAAAAGTGATGCTGGGGTAAAGGCTGCAGGGGAAGAGAAAGTTACGTCGGATACGAAAGCAGATAAAAAAGCTACTAATGAGAAGACAAAATCACCAGAAACAGAGGCAGTTGAGAAAGACAAAGAAGTTAGTGTCAGTTTATTGAATATACAGGTTGGCCTTGTGCGCAAAGCTTGGAAGCATCCGTCTGCTGATAGTTTACTGGTCGAGGAGATAGATGTTGGAGAAGCTAAATTACGGCAAGTGGTCAGCGGCTTGGCAAAATATATCAGTCCAGAGGAGTTAACAAACCGTCATGTTGTGCTGATTACGAATGTGAAACCTGGGAAATTACGGGATGTGATGTCACAAGGACTGGTGCTATGTGCTTCTAGTGAAGATCACACCCTAGTAGAGCCTTTGCTTCCTCCACAAGGAGCCAAACCGGGGGAACGTGTATCATTTTCTGGGGTTGATGGAAAGCCAGAAGATGTGCTGAACCCAAAAAAGAAACAGCTGGACAAGATCACACCGAATCTTTTCACTGACGATAAGGGCGTGGCTACCTTCAAGGGTATCCCGTTTATGACCTCGGCCGGGCCTTGTACATCATCCATCCCCAAGGGAACTATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C tRNA_bind

Protein Analysis

408

Amino Acids

43.62

Weight (kDa)

7.06

Isoelectric Point (pI)

26.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arc1p_N_like PF21972 80 - 142 7e-11 Nuclear-export cofactor Arc1p
tRNA_bind PF01588 252 - 347 1.4e-27 Putative tRNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012158)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40660
fragaria_vesca FvH4_7g25960
malus_domestica MD01G1168000.v1.1 MD07G1234000.v1.1
prunus_persica Prupe.2G264400_v2.0.a1
pyrus_communis pycom01g18100 pycom07g21180
rosa_chinensis RchiOBHm_Chr1g0372341
rosa_laevigata RLG00000026906
rosa_multiflora Rmu_sc0001755.1_g000007
rosa_roxburghii Rroxscaffold_4G00285030
rosa_rugosa Rorug01G0371300 Rorug01G0371300 Rorug01G0371300
rosa_samantha Rh1AG379700 Rh1BG343200 Rh1CG356700 Rh1DG374400
rosa_wichuraiana Rw1G033380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 773
Acc36I ACCTGC 1 cut(s) 509
AccB1I GGYRCC 1 cut(s) 158
AciI CCGC 5 cut(s) 338, 389, 458, 573, 858
AclWI GGATC 1 cut(s) 71
AcoI YGGCCR 1 cut(s) 1179
AcsI RAATTY 1 cut(s) 473
AcuI CTGAAG 1 cut(s) 333
AfaI GTAC 5 cut(s) 19, 128, 138, 444, 1192
AfiI CCNNNNNNNGG 4 cut(s) 424, 1019, 1032, 1182
AflIII ACRYGT 1 cut(s) 1039
AgsI TTSAA 5 cut(s) 259, 366, 540, 751, 1156
AjnI CCWGG 2 cut(s) 228, 928
AjuI GAANNNNNNNTTGG 2 cut(s) 251, 283
AluBI AGCT 9 cut(s) 8, 53, 123, 245, 317, 677, 779, 836, 1102
AluI AGCT 9 cut(s) 8, 53, 123, 245, 317, 677, 779, 836, 1102
AlwI GGATC 1 cut(s) 71
AoxI GGCC 3 cut(s) 763, 1179, 1184
ApeKI GCWGC 3 cut(s) 5, 242, 629
ApoI RAATTY 1 cut(s) 473
Asp700I GAANNNNTTC 1 cut(s) 1124
AspLEI GCGC 2 cut(s) 169, 774
AspS9I GGNCC 1 cut(s) 1184
AsuC2I CCSGG 2 cut(s) 1032, 1183
AsuHPI GGTGA 3 cut(s) 199, 208, 690
AsuII TTCGAA 1 cut(s) 484
BanI GGYRCC 1 cut(s) 158
BarI GAAGNNNNNNTAC 2 cut(s) 825, 857
BauI CACGAG 1 cut(s) 118
BbsI GAAGAC 2 cut(s) 557, 695
BbvCI CCTCAGC 1 cut(s) 163
BbvI GCAGC 3 cut(s) 17, 254, 616
BccI CCATC 4 cut(s) 173, 396, 1055, 1208
BceAI ACGGC 2 cut(s) 172, 860
BciT130I CCWGG 2 cut(s) 230, 930
BciVI GTATCC 2 cut(s) 649, 1172
BcnI CCSGG 2 cut(s) 1032, 1183
BfaI CTAG 3 cut(s) 584, 978, 995
BfmI CTRYAG 3 cut(s) 219, 240, 630
BfuAI ACCTGC 1 cut(s) 509
BfuI GTATCC 2 cut(s) 649, 1172
BisI GCNGC 5 cut(s) 6, 243, 389, 630, 859
BlsI GCNGC 5 cut(s) 7, 244, 390, 631, 860
Bme1390I CCNGG 4 cut(s) 230, 930, 1032, 1183
BmgT120I GGNCC 1 cut(s) 1184
BmiI GGNNCC 2 cut(s) 160, 1024
BmrFI CCNGG 4 cut(s) 230, 930, 1032, 1183
BmsI GCATC 3 cut(s) 310, 604, 796
BpiI GAAGAC 2 cut(s) 557, 695
Bpu10I CCTNAGC 2 cut(s) 9, 163
Bpu14I TTCGAA 1 cut(s) 484
BpuMI CCSGG 2 cut(s) 1032, 1183
BsaJI CCNNGG 6 cut(s) 228, 267, 929, 1031, 1176, 1206
Bsc4I CCNNNNNNNGG 4 cut(s) 424, 1019, 1032, 1182
Bse1I ACTGG 2 cut(s) 813, 966
Bse3DI GCAATG 1 cut(s) 301
BseBI CCWGG 2 cut(s) 230, 930
BseDI CCNNGG 6 cut(s) 228, 267, 929, 1031, 1176, 1206
BseGI GGATG 6 cut(s) 414, 499, 787, 949, 1196, 1200
BseLI CCNNNNNNNGG 4 cut(s) 424, 1019, 1032, 1182
BseMI GCAATG 1 cut(s) 301
BseMII CTCAG 2 cut(s) 177, 438
BseNI ACTGG 2 cut(s) 813, 966
BseRI GAGGAG 2 cut(s) 830, 899
BseX3I CGGCCG 1 cut(s) 1179
BseXI GCAGC 3 cut(s) 17, 254, 616
BseYI CCCAGC 2 cut(s) 502, 617
BsgI GTGCAG 1 cut(s) 537
Bsh1285I CGRYCG 1 cut(s) 1182
BshFI GGCC 3 cut(s) 765, 1181, 1186
BshNI GGYRCC 1 cut(s) 158
BsiEI CGRYCG 1 cut(s) 1182
BsiSI CCGG 3 cut(s) 92, 1031, 1182
BslFI GGGAC 1 cut(s) 452
BslI CCNNNNNNNGG 4 cut(s) 424, 1019, 1032, 1182
BsmFI GGGAC 1 cut(s) 452
BsmI GAATGC 1 cut(s) 343
BsnI GGCC 3 cut(s) 765, 1181, 1186
Bsp119I TTCGAA 1 cut(s) 484
Bsp1407I TGTACA 1 cut(s) 1190
Bsp143I GATC 3 cut(s) 76, 985, 1110
BspACI CCGC 5 cut(s) 338, 389, 458, 573, 858
BspANI GGCC 3 cut(s) 765, 1181, 1186
BspCNI CTCAG 2 cut(s) 176, 439
BspLI GGNNCC 2 cut(s) 160, 1024
BspMAI CTGCAG 2 cut(s) 244, 634
BspMI ACCTGC 1 cut(s) 509
BspPI GGATC 1 cut(s) 71
BspT104I TTCGAA 1 cut(s) 484
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 301
BsrGI TGTACA 1 cut(s) 1190
BsrI ACTGG 2 cut(s) 813, 966
BssECI CCNNGG 6 cut(s) 228, 267, 929, 1031, 1176, 1206
BssMI GATC 3 cut(s) 76, 985, 1110
BssSI CACGAG 1 cut(s) 118
BssT1I CCWWGG 2 cut(s) 267, 1206
Bst2BI CACGAG 1 cut(s) 118
Bst2UI CCWGG 2 cut(s) 230, 930
Bst4CI ACNGT 3 cut(s) 136, 556, 899
Bst6I CTCTTC 1 cut(s) 633
BstAPI GCANNNNNTGC 3 cut(s) 239, 385, 515
BstAUI TGTACA 1 cut(s) 1190
BstBI TTCGAA 1 cut(s) 484
BstC8I GCNNGC 2 cut(s) 106, 235
BstDEI CTNAG 3 cut(s) 9, 163, 447
BstENI CCTNNNNNAGG 1 cut(s) 1017
BstF5I GGATG 6 cut(s) 414, 499, 787, 949, 1196, 1200
BstHHI GCGC 2 cut(s) 169, 774
BstKTI GATC 3 cut(s) 79, 988, 1113
BstMBI GATC 3 cut(s) 76, 985, 1110
BstMCI CGRYCG 1 cut(s) 1182
BstMWI GCNNNNNNNGC 4 cut(s) 239, 385, 515, 771
BstNI CCWGG 2 cut(s) 230, 930
BstNSI RCATGY 1 cut(s) 237
BstSCI CCNGG 4 cut(s) 228, 928, 1030, 1181
BstSFI CTRYAG 3 cut(s) 219, 240, 630
BstV1I GCAGC 3 cut(s) 17, 254, 616
BstV2I GAAGAC 2 cut(s) 557, 695
BstZI CGGCCG 1 cut(s) 1179
BsuI GTATCC 2 cut(s) 649, 1172
BsuRI GGCC 3 cut(s) 765, 1181, 1186
BtgZI GCGATG 2 cut(s) 85, 123
BtsCI GGATG 6 cut(s) 414, 499, 787, 949, 1196, 1200
BtsIMutI CAGTG 2 cut(s) 288, 1128
BveI ACCTGC 1 cut(s) 509
Cac8I GCNNGC 2 cut(s) 106, 235
CfoI GCGC 2 cut(s) 169, 774
Cfr13I GGNCC 1 cut(s) 1184
Csp6I GTAC 5 cut(s) 18, 127, 137, 443, 1191
CviAII CATG 2 cut(s) 234, 902
CviQI GTAC 5 cut(s) 18, 127, 137, 443, 1191
DdeI CTNAG 3 cut(s) 9, 163, 447
DpnI GATC 3 cut(s) 78, 987, 1112
DpnII GATC 3 cut(s) 76, 985, 1110
EaeI YGGCCR 1 cut(s) 1179
EagI CGGCCG 1 cut(s) 1179
Eam1104I CTCTTC 1 cut(s) 633
EarI CTCTTC 1 cut(s) 633
EclXI CGGCCG 1 cut(s) 1179
Eco130I CCWWGG 2 cut(s) 267, 1206
Eco52I CGGCCG 1 cut(s) 1179
Eco57I CTGAAG 1 cut(s) 333
EcoNI CCTNNNNNAGG 1 cut(s) 1017
EcoRII CCWGG 2 cut(s) 228, 928
EcoT14I CCWWGG 2 cut(s) 267, 1206
ErhI CCWWGG 2 cut(s) 267, 1206
FaeI CATG 2 cut(s) 237, 905
FaiI YATR 7 cut(s) 204, 235, 755, 873, 903, 970, 1172
FaqI GGGAC 1 cut(s) 452
FatI CATG 2 cut(s) 233, 901
Fnu4HI GCNGC 5 cut(s) 6, 243, 389, 630, 859
FokI GGATG 6 cut(s) 401, 506, 774, 956, 1183, 1187
Fsp4HI GCNGC 5 cut(s) 6, 243, 389, 630, 859
FspBI CTAG 3 cut(s) 584, 978, 995
FspI TGCGCA 1 cut(s) 773
GlaI GCGC 2 cut(s) 168, 773
GluI GCNGC 5 cut(s) 6, 243, 389, 630, 859
GsaI CCCAGC 2 cut(s) 506, 621
HaeIII GGCC 3 cut(s) 765, 1181, 1186
HapII CCGG 3 cut(s) 92, 1031, 1182
HhaI GCGC 2 cut(s) 169, 774
Hin1II CATG 2 cut(s) 237, 905
Hin6I GCGC 2 cut(s) 167, 772
HinP1I GCGC 2 cut(s) 167, 772
HincII GTYRAC 1 cut(s) 891
HindII GTYRAC 1 cut(s) 891
HindIII AAGCTT 1 cut(s) 777
HinfI GANTC 4 cut(s) 43, 224, 536, 1120
HpaI GTTAAC 1 cut(s) 891
HpaII CCGG 3 cut(s) 92, 1031, 1182
HphI GGTGA 3 cut(s) 199, 208, 690
Hpy166II GTNNAC 4 cut(s) 175, 303, 806, 891
Hpy188I TCNGA 2 cut(s) 313, 655
Hpy188III TCNNGA 5 cut(s) 74, 118, 248, 418, 881
Hpy8I GTNNAC 4 cut(s) 175, 303, 806, 891
Hpy99I CGWCG 1 cut(s) 655
HpyAV CCTTC 3 cut(s) 245, 460, 1162
HpyCH4III ACNGT 3 cut(s) 136, 556, 899
HpyCH4IV ACGT 4 cut(s) 276, 441, 650, 1039
HpyCH4V TGCA 7 cut(s) 60, 206, 242, 343, 518, 545, 632
HpyF10VI GCNNNNNNNGC 4 cut(s) 239, 385, 515, 771
HpyF3I CTNAG 3 cut(s) 9, 163, 447
HpySE526I ACGT 4 cut(s) 276, 441, 650, 1039
Hsp92II CATG 2 cut(s) 237, 905
HspAI GCGC 2 cut(s) 167, 772
KspAI GTTAAC 1 cut(s) 891
Kzo9I GATC 3 cut(s) 76, 985, 1110
LmnI GCTCC 2 cut(s) 506, 1022
Lsp1109I GCAGC 3 cut(s) 17, 254, 616
LweI GCATC 3 cut(s) 310, 604, 796
MaeI CTAG 3 cut(s) 584, 978, 995
MaeII ACGT 4 cut(s) 276, 441, 650, 1039
MaeIII GTNAC 3 cut(s) 115, 646, 951
MalI GATC 3 cut(s) 78, 987, 1112
MboI GATC 3 cut(s) 76, 985, 1110
MboII GAAGA 7 cut(s) 339, 463, 562, 650, 700, 995, 1085
MfeI CAATTG 1 cut(s) 38
MluCI AATT 7 cut(s) 38, 190, 473, 497, 531, 839, 935
MmeI TCCRAC 3 cut(s) 471, 633, 808
MnlI CCTC 8 cut(s) 172, 202, 319, 703, 808, 877, 1023, 1186
MroXI GAANNNNTTC 1 cut(s) 1124
MseI TTAA 1 cut(s) 890
MspA1I CMGCKG 2 cut(s) 858, 1102
MspI CCGG 3 cut(s) 92, 1031, 1182
MspR9I CCNGG 4 cut(s) 230, 930, 1032, 1183
MunI CAATTG 1 cut(s) 38
Mva1269I GAATGC 1 cut(s) 343
MvaI CCWGG 2 cut(s) 230, 930
MwoI GCNNNNNNNGC 4 cut(s) 239, 385, 515, 771
NciI CCSGG 2 cut(s) 1032, 1183
NdeII GATC 3 cut(s) 76, 985, 1110
NlaIII CATG 2 cut(s) 237, 905
NlaIV GGNNCC 2 cut(s) 160, 1024
NmeAIII GCCGAG 1 cut(s) 1157
NmuCI GTSAC 2 cut(s) 115, 951
NsbI TGCGCA 1 cut(s) 773
NspI RCATGY 1 cut(s) 237
NspV TTCGAA 1 cut(s) 484
PaeI GCATGC 1 cut(s) 237
PcsI WCGNNNNNNNCGW 2 cut(s) 656, 1140
PctI GAATGC 1 cut(s) 343
PdmI GAANNNNTTC 1 cut(s) 1124
PfeI GAWTC 4 cut(s) 43, 224, 536, 1120
PkrI GCNGC 5 cut(s) 7, 244, 390, 631, 860
Psp6I CCWGG 2 cut(s) 228, 928
PspFI CCCAGC 2 cut(s) 502, 617
PspGI CCWGG 2 cut(s) 228, 928
PspN4I GGNNCC 2 cut(s) 160, 1024
PspPI GGNCC 1 cut(s) 1184
PstI CTGCAG 2 cut(s) 244, 634
PvuII CAGCTG 1 cut(s) 1102
RsaI GTAC 5 cut(s) 19, 128, 138, 444, 1192
RsaNI GTAC 5 cut(s) 18, 127, 137, 443, 1191
SaqAI TTAA 1 cut(s) 890
SatI GCNGC 5 cut(s) 6, 243, 389, 630, 859
Sau3AI GATC 3 cut(s) 76, 985, 1110
Sau96I GGNCC 1 cut(s) 1184
ScrFI CCNGG 4 cut(s) 230, 930, 1032, 1183
SfaNI GCATC 3 cut(s) 310, 604, 796
SfcI CTRYAG 3 cut(s) 219, 240, 630
SfuI TTCGAA 1 cut(s) 484
SphI GCATGC 1 cut(s) 237
Sse9I AATT 7 cut(s) 38, 190, 473, 497, 531, 839, 935
SsiI CCGC 5 cut(s) 338, 389, 458, 573, 858
SspMI CTAG 3 cut(s) 584, 978, 995
StyD4I CCNGG 4 cut(s) 228, 928, 1030, 1181
StyI CCWWGG 2 cut(s) 267, 1206
TaaI ACNGT 3 cut(s) 136, 556, 899
TaiI ACGT 4 cut(s) 279, 444, 653, 1042
TaqI TCGA 4 cut(s) 75, 249, 484, 813
TasI AATT 7 cut(s) 38, 190, 473, 497, 531, 839, 935
TatI WGTACW 2 cut(s) 126, 1190
TauI GCSGC 2 cut(s) 391, 861
TfiI GAWTC 4 cut(s) 43, 224, 536, 1120
Tru1I TTAA 1 cut(s) 890
Tru9I TTAA 1 cut(s) 890
TscAI CASTG 2 cut(s) 288, 1135
TseFI GTSAC 2 cut(s) 115, 951
TseI GCWGC 3 cut(s) 5, 242, 629
Tsp45I GTSAC 2 cut(s) 115, 951
TspDTI ATGAA 3 cut(s) 126, 276, 510
TspGWI ACGGA 1 cut(s) 780
TspRI CASTG 2 cut(s) 288, 1135
XagI CCTNNNNNAGG 1 cut(s) 1017
XapI RAATTY 1 cut(s) 473
XceI RCATGY 1 cut(s) 237
XmnI GAANNNNTTC 1 cut(s) 1124
XspI CTAG 3 cut(s) 584, 978, 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.