pycom07g21180

Putative tRNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0012158
Physical Location & Seq
Forward (+)
23003700 .. 23006321
2622 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g21180.9

Sequence Viewer

Length: 621 bp
ATGCTGGGTTTAAATCACCATAAGCTGAATAACCATATTGCGCTTAATCAACTAAAGAAAGCAGATAAAAAAGCTACTAATGAGAAGACAAAATCACCAGAAACAGAGGCAGTTGAGAAAGACAAAGAAGTTTGTGTCAGTTTGTTGAATATACAGGTTGGCCTCGTGCGCAAAGCTTGGAAGCATCCGTCTGCTGATAGTTTACTGGTCGAGGAGATAGATGTCGGAGAAGCTAAATTACGGCAAGTGGTCAGCGGCTTGGCAAAATATATCAGTCCAGAGGAGTTAATAAACCGTCATGTTGTGCTGATTACGAATGTGAAACCTGGGAAATTACGGGATGTGATGTCACAAGGACTGGTGCTATGTGCTTCTAGTGAAGATCACACCCTAGTAGAGCCCTTGCTACCTCCACAAGGAGCCAAACCGGGGGAACGTGTATCATTTTCTGGGGTTGATGGAAAGCCAGAAGATGTGCTGAACCCAAAAAAGAAACAGCTGGACAAGATCACACCGAATCTTTTCACTGACGATAAGGGTGTGGCTACCTTCAGGGGTATCCCGTTTATGACCTCGGCCGGGCCTTGTACATCATCCATCCCCAAGGGAACTATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C tRNA_bind

Protein Analysis

207

Amino Acids

22.44

Weight (kDa)

9.04

Isoelectric Point (pI)

32.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012158)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40660
fragaria_vesca FvH4_7g25960
malus_domestica MD01G1168000.v1.1 MD07G1234000.v1.1
prunus_persica Prupe.2G264400_v2.0.a1
pyrus_communis pycom01g18100 pycom07g21180
rosa_chinensis RchiOBHm_Chr1g0372341
rosa_laevigata RLG00000026906
rosa_multiflora Rmu_sc0001755.1_g000007
rosa_roxburghii Rroxscaffold_4G00285030
rosa_rugosa Rorug01G0371300 Rorug01G0371300 Rorug01G0371300
rosa_samantha Rh1AG379700 Rh1BG343200 Rh1CG356700 Rh1DG374400
rosa_wichuraiana Rw1G033380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 170
AciI CCGC 1 cut(s) 255
AcoI YGGCCR 1 cut(s) 576
AcuI CTGAAG 1 cut(s) 535
AfaI GTAC 1 cut(s) 589
AfiI CCNNNNNNNGG 3 cut(s) 416, 429, 579
AflIII ACRYGT 1 cut(s) 436
AgsI TTSAA 1 cut(s) 148
AjnI CCWGG 1 cut(s) 325
AluBI AGCT 5 cut(s) 25, 74, 176, 233, 499
AluI AGCT 5 cut(s) 25, 74, 176, 233, 499
AoxI GGCC 3 cut(s) 160, 576, 581
Asp700I GAANNNNTTC 1 cut(s) 521
AspLEI GCGC 2 cut(s) 43, 171
AspS9I GGNCC 1 cut(s) 581
AsuC2I CCSGG 2 cut(s) 429, 580
AsuHPI GGTGA 2 cut(s) 8, 87
BanII GRGCYC 1 cut(s) 402
BarI GAAGNNNNNNTAC 2 cut(s) 222, 254
BauI CACGAG 1 cut(s) 164
BbsI GAAGAC 1 cut(s) 92
BccI CCATC 2 cut(s) 452, 605
BceAI ACGGC 1 cut(s) 257
BciT130I CCWGG 1 cut(s) 327
BciVI GTATCC 1 cut(s) 569
BcnI CCSGG 2 cut(s) 429, 580
BfaI CTAG 2 cut(s) 375, 392
BfuI GTATCC 1 cut(s) 569
BisI GCNGC 1 cut(s) 256
BlsI GCNGC 1 cut(s) 257
Bme1390I CCNGG 3 cut(s) 327, 429, 580
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 1 cut(s) 421
BmrFI CCNGG 3 cut(s) 327, 429, 580
BmsI GCATC 1 cut(s) 193
BpiI GAAGAC 1 cut(s) 92
BpuMI CCSGG 2 cut(s) 429, 580
BsaJI CCNNGG 4 cut(s) 326, 428, 573, 603
Bsc4I CCNNNNNNNGG 3 cut(s) 416, 429, 579
Bse1I ACTGG 2 cut(s) 210, 363
BseBI CCWGG 1 cut(s) 327
BseDI CCNNGG 4 cut(s) 326, 428, 573, 603
BseGI GGATG 4 cut(s) 184, 346, 593, 597
BseLI CCNNNNNNNGG 3 cut(s) 416, 429, 579
BseNI ACTGG 2 cut(s) 210, 363
BseRI GAGGAG 2 cut(s) 227, 296
BseX3I CGGCCG 1 cut(s) 576
BseYI CCCAGC 1 cut(s) 4
Bsh1285I CGRYCG 1 cut(s) 579
BshFI GGCC 3 cut(s) 162, 578, 583
BsiEI CGRYCG 1 cut(s) 579
BsiSI CCGG 2 cut(s) 428, 579
BslI CCNNNNNNNGG 3 cut(s) 416, 429, 579
BsnI GGCC 3 cut(s) 162, 578, 583
Bsp1286I GDGCHC 1 cut(s) 402
Bsp1407I TGTACA 1 cut(s) 587
Bsp143I GATC 2 cut(s) 382, 507
BspACI CCGC 1 cut(s) 255
BspANI GGCC 3 cut(s) 162, 578, 583
BspLI GGNNCC 1 cut(s) 421
BsrGI TGTACA 1 cut(s) 587
BsrI ACTGG 2 cut(s) 210, 363
BssECI CCNNGG 4 cut(s) 326, 428, 573, 603
BssMI GATC 2 cut(s) 382, 507
BssSI CACGAG 1 cut(s) 164
BssT1I CCWWGG 1 cut(s) 603
Bst2BI CACGAG 1 cut(s) 164
Bst2UI CCWGG 1 cut(s) 327
Bst4CI ACNGT 1 cut(s) 296
BstAUI TGTACA 1 cut(s) 587
BstENI CCTNNNNNAGG 1 cut(s) 414
BstF5I GGATG 4 cut(s) 184, 346, 593, 597
BstHHI GCGC 2 cut(s) 43, 171
BstKTI GATC 2 cut(s) 385, 510
BstMBI GATC 2 cut(s) 382, 507
BstMCI CGRYCG 1 cut(s) 579
BstMWI GCNNNNNNNGC 1 cut(s) 168
BstNI CCWGG 1 cut(s) 327
BstSCI CCNGG 3 cut(s) 325, 427, 578
BstV2I GAAGAC 1 cut(s) 92
BstZI CGGCCG 1 cut(s) 576
BsuI GTATCC 1 cut(s) 569
BsuRI GGCC 3 cut(s) 162, 578, 583
BtsCI GGATG 4 cut(s) 184, 346, 593, 597
BtsIMutI CAGTG 1 cut(s) 525
CfoI GCGC 2 cut(s) 43, 171
Cfr13I GGNCC 1 cut(s) 581
Csp6I GTAC 1 cut(s) 588
CviAII CATG 1 cut(s) 299
CviQI GTAC 1 cut(s) 588
DpnI GATC 2 cut(s) 384, 509
DpnII GATC 2 cut(s) 382, 507
DraI TTTAAA 1 cut(s) 12
EaeI YGGCCR 1 cut(s) 576
EagI CGGCCG 1 cut(s) 576
EclXI CGGCCG 1 cut(s) 576
Eco130I CCWWGG 1 cut(s) 603
Eco24I GRGCYC 1 cut(s) 402
Eco52I CGGCCG 1 cut(s) 576
Eco57I CTGAAG 1 cut(s) 535
EcoNI CCTNNNNNAGG 1 cut(s) 414
EcoRII CCWGG 1 cut(s) 325
EcoT14I CCWWGG 1 cut(s) 603
EcoT38I GRGCYC 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 603
FaeI CATG 1 cut(s) 302
FaiI YATR 7 cut(s) 21, 36, 152, 270, 300, 367, 569
FatI CATG 1 cut(s) 298
Fnu4HI GCNGC 1 cut(s) 256
FokI GGATG 4 cut(s) 171, 353, 580, 584
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 1 cut(s) 256
FspBI CTAG 2 cut(s) 375, 392
FspI TGCGCA 1 cut(s) 170
GlaI GCGC 2 cut(s) 42, 170
GluI GCNGC 1 cut(s) 256
GsaI CCCAGC 1 cut(s) 8
HaeIII GGCC 3 cut(s) 162, 578, 583
HapII CCGG 2 cut(s) 428, 579
HhaI GCGC 2 cut(s) 43, 171
Hin1II CATG 1 cut(s) 302
Hin6I GCGC 2 cut(s) 41, 169
HinP1I GCGC 2 cut(s) 41, 169
HindIII AAGCTT 1 cut(s) 174
HinfI GANTC 1 cut(s) 517
HpaII CCGG 2 cut(s) 428, 579
HphI GGTGA 2 cut(s) 8, 87
Hpy166II GTNNAC 1 cut(s) 203
Hpy188I TCNGA 1 cut(s) 227
Hpy188III TCNNGA 1 cut(s) 278
Hpy8I GTNNAC 1 cut(s) 203
HpyAV CCTTC 1 cut(s) 559
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4IV ACGT 1 cut(s) 436
HpyF10VI GCNNNNNNNGC 1 cut(s) 168
HpySE526I ACGT 1 cut(s) 436
Hsp92II CATG 1 cut(s) 302
HspAI GCGC 2 cut(s) 41, 169
Kzo9I GATC 2 cut(s) 382, 507
LmnI GCTCC 1 cut(s) 419
LweI GCATC 1 cut(s) 193
MaeI CTAG 2 cut(s) 375, 392
MaeII ACGT 1 cut(s) 436
MaeIII GTNAC 1 cut(s) 348
MalI GATC 2 cut(s) 384, 509
MboI GATC 2 cut(s) 382, 507
MboII GAAGA 3 cut(s) 97, 392, 482
MhlI GDGCHC 1 cut(s) 402
MluCI AATT 2 cut(s) 236, 332
MmeI TCCRAC 1 cut(s) 205
MnlI CCTC 6 cut(s) 100, 173, 205, 274, 420, 583
MroXI GAANNNNTTC 1 cut(s) 521
MseI TTAA 3 cut(s) 11, 45, 287
MspA1I CMGCKG 2 cut(s) 255, 499
MspI CCGG 2 cut(s) 428, 579
MspR9I CCNGG 3 cut(s) 327, 429, 580
MvaI CCWGG 1 cut(s) 327
MwoI GCNNNNNNNGC 1 cut(s) 168
NciI CCSGG 2 cut(s) 429, 580
NdeII GATC 2 cut(s) 382, 507
NlaIII CATG 1 cut(s) 302
NlaIV GGNNCC 1 cut(s) 421
NmeAIII GCCGAG 1 cut(s) 554
NmuCI GTSAC 1 cut(s) 348
NsbI TGCGCA 1 cut(s) 170
PdmI GAANNNNTTC 1 cut(s) 521
PfeI GAWTC 1 cut(s) 517
PkrI GCNGC 1 cut(s) 257
Psp6I CCWGG 1 cut(s) 325
PspFI CCCAGC 1 cut(s) 4
PspGI CCWGG 1 cut(s) 325
PspN4I GGNNCC 1 cut(s) 421
PspPI GGNCC 1 cut(s) 581
PvuII CAGCTG 1 cut(s) 499
RsaI GTAC 1 cut(s) 589
RsaNI GTAC 1 cut(s) 588
SaqAI TTAA 3 cut(s) 11, 45, 287
SatI GCNGC 1 cut(s) 256
Sau3AI GATC 2 cut(s) 382, 507
Sau96I GGNCC 1 cut(s) 581
ScrFI CCNGG 3 cut(s) 327, 429, 580
SduI GDGCHC 1 cut(s) 402
SfaNI GCATC 1 cut(s) 193
Sse9I AATT 2 cut(s) 236, 332
SsiI CCGC 1 cut(s) 255
SspMI CTAG 2 cut(s) 375, 392
StyD4I CCNGG 3 cut(s) 325, 427, 578
StyI CCWWGG 1 cut(s) 603
TaaI ACNGT 1 cut(s) 296
TaiI ACGT 1 cut(s) 439
TaqI TCGA 1 cut(s) 210
TasI AATT 2 cut(s) 236, 332
TatI WGTACW 1 cut(s) 587
TauI GCSGC 1 cut(s) 258
TfiI GAWTC 1 cut(s) 517
Tru1I TTAA 3 cut(s) 11, 45, 287
Tru9I TTAA 3 cut(s) 11, 45, 287
TscAI CASTG 1 cut(s) 532
TseFI GTSAC 1 cut(s) 348
Tsp45I GTSAC 1 cut(s) 348
TspGWI ACGGA 1 cut(s) 177
TspRI CASTG 1 cut(s) 532
XagI CCTNNNNNAGG 1 cut(s) 414
XmnI GAANNNNTTC 1 cut(s) 521
XspI CTAG 2 cut(s) 375, 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.