pycom01g18100

Putative tRNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0012158
Physical Location & Seq
Forward (+)
17224374 .. 17228580
4207 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g18100.6

Sequence Viewer

Length: 1248 bp
ATGGCAGTTAATGGAAGCACCAGCGTAAGCAGAAAGCAGCTGATACTATCAGCTCTCTGCAAATACTTCTCTCTCGATCCTAAATCGTTGCCGGAAAACATCGCTGGCGATGAAGTCACGAGCTTGTACTCAACCGTACTCAAATCAGCCAAAAACGGCGCCGCAGGGGAACTGAAACATGGGGTGGTGAATTGGATAAGATATGCAGAGGGTTTTCCTACAGATTCCCAGGCATGCTCTGGAGCTCTCAAGGGGTTGAATGAAACCTTGGCTGATGTGTCAGTGCTTTTGGGCAATGGGTTCACCCCCTCTGAAGCTGATGCTGTTGTGTTTGCTGCAGTGCATTCTTCTGTGATTGGACTTTCAAATTCAGATAGGCAAAAGTTGCCGCATTTGTTGAGATGGGTGGATTACATCCAGAACAAGGTGGATTTCGGGGACATACTTGAGAAGATAGTGATAGAGAAGGATAAATTTGATTTTCGAAAGTTGGATGAATTGCTGGGAACAAAAAGTGCGGGTAAGGTGGAAATTGATTCAAATGCAAAGAAGACTGTTCAAAGCGCAAAAAGTGCAGACAAGCCAGAGGCAGACCTGAACACAAAGAAAAGTGATGCTGGGGTAAAGGCAGCAGGAGATGAGAAAATTATTTCGGATAAGAAAGCAGATAAGAAAGCAGATAAGAAAGCAGATAAGAAAGCTACTGCAGAGAAGAAAAAATTGCCAGAAACAGAGGCAGTTGAGAAAGACAAAGAAGTAAGTGTCAGTTTATTGAATATACAGGTTGGCCTCGTGCGCAAAGCTTGGAAGCATCCGTCTGCTGATAGTTTACTGGTTGAGGAGATAGATGTCGGAGAAGCGAAATTACGGCAAGTGGTTAGCGGCTTGGCAAAATATATCAGTCCAGAGGAGTTGACAAACCGTCATGTTGTGCTGATTACGAATGTGAAACCTGGGAAGTTACGGGATGTGATGTCACAAGGACTGGTGCTATGTGCTTCTAAGGAAGATCACACCCTTGTAGAGCCTTTGCTCCCTCCACAAGGGGCTAAACCAGGGGAACGTGTGTCATTTTGTGGGATTGATGGAAAGCCAGAAGATGTTCTGAACCCAAAAAAGAAACAGCTGGACAAGATCACACCACATCTTTTCACTGACGATAAGGGCGTGGCTACCTTCAAGGGTATTCCGTTTATGACCTCGGCTGGGCCTTGTACATCATCCATCCCCAAGGCAACCATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C tRNA_bind

Protein Analysis

416

Amino Acids

44.75

Weight (kDa)

8.76

Isoelectric Point (pI)

24.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012158)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40660
fragaria_vesca FvH4_7g25960
malus_domestica MD01G1168000.v1.1 MD07G1234000.v1.1
prunus_persica Prupe.2G264400_v2.0.a1
pyrus_communis pycom01g18100 pycom07g21180
rosa_chinensis RchiOBHm_Chr1g0372341
rosa_laevigata RLG00000026906
rosa_multiflora Rmu_sc0001755.1_g000007
rosa_roxburghii Rroxscaffold_4G00285030
rosa_rugosa Rorug01G0371300 Rorug01G0371300 Rorug01G0371300
rosa_samantha Rh1AG379700 Rh1BG343200 Rh1CG356700 Rh1DG374400
rosa_wichuraiana Rw1G033380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 797
AccB1I GGYRCC 1 cut(s) 158
AciI CCGC 4 cut(s) 162, 389, 518, 882
AclWI GGATC 1 cut(s) 71
AcsI RAATTY 2 cut(s) 367, 473
AcuI CTGAAG 1 cut(s) 333
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 3 cut(s) 128, 138, 1216
AfiI CCNNNNNNNGG 3 cut(s) 424, 1043, 1206
AflIII ACRYGT 1 cut(s) 1063
AgsI TTSAA 6 cut(s) 259, 366, 540, 560, 775, 1180
AhdI GACNNNNNGTC 1 cut(s) 921
AjnI CCWGG 3 cut(s) 228, 952, 1054
AjuI GAANNNNNNNTTGG 2 cut(s) 251, 283
AluBI AGCT 8 cut(s) 40, 53, 123, 245, 317, 701, 803, 1126
AluI AGCT 8 cut(s) 40, 53, 123, 245, 317, 701, 803, 1126
Alw21I GWGCWC 1 cut(s) 247
AlwI GGATC 1 cut(s) 71
AoxI GGCC 2 cut(s) 787, 1208
ApeKI GCWGC 3 cut(s) 37, 335, 629
ApoI RAATTY 2 cut(s) 367, 473
Asp700I GAANNNNTTC 1 cut(s) 1101
AspLEI GCGC 3 cut(s) 161, 566, 798
AspS9I GGNCC 1 cut(s) 1208
AsuHPI GGTGA 2 cut(s) 199, 295
AsuII TTCGAA 1 cut(s) 484
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 247
BarI GAAGNNNNNNTAC 2 cut(s) 849, 881
BauI CACGAG 2 cut(s) 118, 791
BbsI GAAGAC 1 cut(s) 557
Bbv12I GWGCWC 1 cut(s) 247
BbvI GCAGC 3 cut(s) 49, 322, 641
BccI CCATC 4 cut(s) 396, 1079, 1232, 1247
BceAI ACGGC 2 cut(s) 172, 884
BciT130I CCWGG 3 cut(s) 230, 954, 1056
BfmI CTRYAG 3 cut(s) 219, 336, 705
BfoI RGCGCY 1 cut(s) 162
BisI GCNGC 6 cut(s) 38, 162, 336, 389, 630, 883
BlsI GCNGC 6 cut(s) 39, 163, 337, 390, 631, 884
Bme1390I CCNGG 3 cut(s) 230, 954, 1056
BmeRI GACNNNNNGTC 1 cut(s) 921
BmgT120I GGNCC 1 cut(s) 1208
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 3 cut(s) 230, 954, 1056
BmsI GCATC 3 cut(s) 310, 604, 820
BpiI GAAGAC 1 cut(s) 557
BpmI CTGGAG 1 cut(s) 261
Bpu14I TTCGAA 1 cut(s) 484
BpuEI CTTGAG 2 cut(s) 233, 467
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 6 cut(s) 228, 267, 953, 1055, 1200, 1230
Bsc4I CCNNNNNNNGG 3 cut(s) 424, 1043, 1206
Bse1I ACTGG 2 cut(s) 837, 990
Bse3DI GCAATG 1 cut(s) 301
BseBI CCWGG 3 cut(s) 230, 954, 1056
BseDI CCNNGG 6 cut(s) 228, 267, 953, 1055, 1200, 1230
BseGI GGATG 6 cut(s) 414, 499, 811, 973, 1220, 1224
BseLI CCNNNNNNNGG 3 cut(s) 424, 1043, 1206
BseMI GCAATG 1 cut(s) 301
BseNI ACTGG 2 cut(s) 837, 990
BseRI GAGGAG 2 cut(s) 854, 923
BseXI GCAGC 3 cut(s) 49, 322, 641
BseYI CCCAGC 3 cut(s) 502, 617, 1205
BsgI GTGCAG 1 cut(s) 594
BshFI GGCC 2 cut(s) 789, 1210
BshNI GGYRCC 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 247
BsiSI CCGG 1 cut(s) 92
BslFI GGGAC 1 cut(s) 452
BslI CCNNNNNNNGG 3 cut(s) 424, 1043, 1206
BsmFI GGGAC 1 cut(s) 452
BsmI GAATGC 1 cut(s) 343
BsnI GGCC 2 cut(s) 789, 1210
Bsp119I TTCGAA 1 cut(s) 484
Bsp1286I GDGCHC 1 cut(s) 247
Bsp1407I TGTACA 1 cut(s) 1214
Bsp143I GATC 3 cut(s) 76, 1009, 1134
BspACI CCGC 4 cut(s) 162, 389, 518, 882
BspANI GGCC 2 cut(s) 789, 1210
BspLI GGNNCC 1 cut(s) 160
BspMAI CTGCAG 2 cut(s) 340, 709
BspPI GGATC 1 cut(s) 71
BspT104I TTCGAA 1 cut(s) 484
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 301
BsrGI TGTACA 1 cut(s) 1214
BsrI ACTGG 2 cut(s) 837, 990
BssECI CCNNGG 6 cut(s) 228, 267, 953, 1055, 1200, 1230
BssMI GATC 3 cut(s) 76, 1009, 1134
BssNI GRCGYC 1 cut(s) 159
BssSI CACGAG 2 cut(s) 118, 791
BssT1I CCWWGG 2 cut(s) 267, 1230
Bst2BI CACGAG 2 cut(s) 118, 791
Bst2UI CCWGG 3 cut(s) 230, 954, 1056
Bst4CI ACNGT 3 cut(s) 136, 556, 923
BstACI GRCGYC 1 cut(s) 159
BstAPI GCANNNNNTGC 2 cut(s) 385, 572
BstAUI TGTACA 1 cut(s) 1214
BstBI TTCGAA 1 cut(s) 484
BstC8I GCNNGC 2 cut(s) 106, 235
BstDEI CTNAG 1 cut(s) 1002
BstENI CCTNNNNNAGG 1 cut(s) 1041
BstF5I GGATG 6 cut(s) 414, 499, 811, 973, 1220, 1224
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 3 cut(s) 161, 566, 798
BstKTI GATC 3 cut(s) 79, 1012, 1137
BstMBI GATC 3 cut(s) 76, 1009, 1134
BstMWI GCNNNNNNNGC 3 cut(s) 385, 572, 795
BstNI CCWGG 3 cut(s) 230, 954, 1056
BstNSI RCATGY 1 cut(s) 237
BstSCI CCNGG 3 cut(s) 228, 952, 1054
BstSFI CTRYAG 3 cut(s) 219, 336, 705
BstV1I GCAGC 3 cut(s) 49, 322, 641
BstV2I GAAGAC 1 cut(s) 557
BsuRI GGCC 2 cut(s) 789, 1210
BtgZI GCGATG 2 cut(s) 85, 123
BtsCI GGATG 6 cut(s) 414, 499, 811, 973, 1220, 1224
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 3 cut(s) 288, 345, 1152
Cac8I GCNNGC 2 cut(s) 106, 235
CfoI GCGC 3 cut(s) 161, 566, 798
Cfr13I GGNCC 1 cut(s) 1208
Csp6I GTAC 3 cut(s) 127, 137, 1215
CviAII CATG 3 cut(s) 179, 234, 926
CviQI GTAC 3 cut(s) 127, 137, 1215
DdeI CTNAG 1 cut(s) 1002
DinI GGCGCC 1 cut(s) 160
DpnI GATC 3 cut(s) 78, 1011, 1136
DpnII GATC 3 cut(s) 76, 1009, 1134
DriI GACNNNNNGTC 1 cut(s) 921
Eam1105I GACNNNNNGTC 1 cut(s) 921
Ecl136II GAGCTC 1 cut(s) 245
Eco130I CCWWGG 2 cut(s) 267, 1230
Eco24I GRGCYC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 245
Eco57I CTGAAG 1 cut(s) 333
EcoICRI GAGCTC 1 cut(s) 245
EcoNI CCTNNNNNAGG 1 cut(s) 1041
EcoRII CCWGG 3 cut(s) 228, 952, 1054
EcoT14I CCWWGG 2 cut(s) 267, 1230
EcoT38I GRGCYC 1 cut(s) 247
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
ErhI CCWWGG 2 cut(s) 267, 1230
FaeI CATG 3 cut(s) 182, 237, 929
FaiI YATR 9 cut(s) 180, 204, 235, 443, 779, 897, 927, 994, 1196
FaqI GGGAC 1 cut(s) 452
FatI CATG 3 cut(s) 178, 233, 925
FauI CCCGC 1 cut(s) 511
Fnu4HI GCNGC 6 cut(s) 38, 162, 336, 389, 630, 883
FokI GGATG 6 cut(s) 401, 506, 798, 980, 1207, 1211
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 6 cut(s) 38, 162, 336, 389, 630, 883
FspI TGCGCA 1 cut(s) 797
GlaI GCGC 3 cut(s) 160, 565, 797
GluI GCNGC 6 cut(s) 38, 162, 336, 389, 630, 883
GsaI CCCAGC 3 cut(s) 506, 621, 1209
GsuI CTGGAG 1 cut(s) 261
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 2 cut(s) 789, 1210
HapII CCGG 1 cut(s) 92
HhaI GCGC 3 cut(s) 161, 566, 798
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 3 cut(s) 182, 237, 929
Hin6I GCGC 3 cut(s) 159, 564, 796
HinP1I GCGC 3 cut(s) 159, 564, 796
HincII GTYRAC 1 cut(s) 915
HindII GTYRAC 1 cut(s) 915
HindIII AAGCTT 1 cut(s) 801
HinfI GANTC 2 cut(s) 224, 536
HpaII CCGG 1 cut(s) 92
HphI GGTGA 2 cut(s) 199, 295
Hpy166II GTNNAC 3 cut(s) 303, 830, 915
Hpy188I TCNGA 5 cut(s) 313, 373, 655, 854, 1107
Hpy188III TCNNGA 5 cut(s) 74, 118, 240, 418, 905
Hpy8I GTNNAC 3 cut(s) 303, 830, 915
HpyAV CCTTC 2 cut(s) 460, 1186
HpyCH4III ACNGT 3 cut(s) 136, 556, 923
HpyCH4IV ACGT 1 cut(s) 1063
HpyCH4V TGCA 7 cut(s) 60, 206, 338, 343, 545, 575, 707
HpyF10VI GCNNNNNNNGC 3 cut(s) 385, 572, 795
HpyF3I CTNAG 1 cut(s) 1002
HpySE526I ACGT 1 cut(s) 1063
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 3 cut(s) 182, 237, 929
HspAI GCGC 3 cut(s) 159, 564, 796
KasI GGCGCC 1 cut(s) 158
Kzo9I GATC 3 cut(s) 76, 1009, 1134
LmnI GCTCC 2 cut(s) 242, 1038
Lsp1109I GCAGC 3 cut(s) 49, 322, 641
LweI GCATC 3 cut(s) 310, 604, 820
MaeII ACGT 1 cut(s) 1063
MaeIII GTNAC 3 cut(s) 115, 960, 975
MalI GATC 3 cut(s) 78, 1011, 1136
MboI GATC 3 cut(s) 76, 1009, 1134
MboII GAAGA 6 cut(s) 339, 463, 562, 724, 1019, 1109
MhlI GDGCHC 1 cut(s) 247
MluCI AATT 8 cut(s) 190, 367, 473, 497, 531, 645, 719, 863
Mly113I GGCGCC 1 cut(s) 159
MmeI TCCRAC 2 cut(s) 471, 832
MnlI CCTC 9 cut(s) 202, 319, 580, 727, 800, 832, 901, 1047, 1210
MroXI GAANNNNTTC 1 cut(s) 1101
MseI TTAA 1 cut(s) 9
MspA1I CMGCKG 2 cut(s) 40, 1126
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 3 cut(s) 230, 954, 1056
Mva1269I GAATGC 1 cut(s) 343
MvaI CCWGG 3 cut(s) 230, 954, 1056
MwoI GCNNNNNNNGC 3 cut(s) 385, 572, 795
NarI GGCGCC 1 cut(s) 159
NdeII GATC 3 cut(s) 76, 1009, 1134
NlaIII CATG 3 cut(s) 182, 237, 929
NlaIV GGNNCC 1 cut(s) 160
NmeAIII GCCGAG 1 cut(s) 1181
NmuCI GTSAC 2 cut(s) 115, 975
NsbI TGCGCA 1 cut(s) 797
NspI RCATGY 1 cut(s) 237
NspV TTCGAA 1 cut(s) 484
PaeI GCATGC 1 cut(s) 237
PcsI WCGNNNNNNNCGW 1 cut(s) 1164
PctI GAATGC 1 cut(s) 343
PdmI GAANNNNTTC 1 cut(s) 1101
PfeI GAWTC 2 cut(s) 224, 536
PkrI GCNGC 6 cut(s) 39, 163, 337, 390, 631, 884
PluTI GGCGCC 1 cut(s) 162
Psp124BI GAGCTC 1 cut(s) 247
Psp6I CCWGG 3 cut(s) 228, 952, 1054
PspFI CCCAGC 3 cut(s) 502, 617, 1205
PspGI CCWGG 3 cut(s) 228, 952, 1054
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 1 cut(s) 1208
PstI CTGCAG 2 cut(s) 340, 709
PvuII CAGCTG 2 cut(s) 40, 1126
RsaI GTAC 3 cut(s) 128, 138, 1216
RsaNI GTAC 3 cut(s) 127, 137, 1215
SacI GAGCTC 1 cut(s) 247
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 6 cut(s) 38, 162, 336, 389, 630, 883
Sau3AI GATC 3 cut(s) 76, 1009, 1134
Sau96I GGNCC 1 cut(s) 1208
ScrFI CCNGG 3 cut(s) 230, 954, 1056
SduI GDGCHC 1 cut(s) 247
SfaNI GCATC 3 cut(s) 310, 604, 820
SfcI CTRYAG 3 cut(s) 219, 336, 705
SfoI GGCGCC 1 cut(s) 160
SfuI TTCGAA 1 cut(s) 484
SmlI CTYRAG 2 cut(s) 248, 446
SmoI CTYRAG 2 cut(s) 248, 446
SphI GCATGC 1 cut(s) 237
Sse9I AATT 8 cut(s) 190, 367, 473, 497, 531, 645, 719, 863
SsiI CCGC 4 cut(s) 162, 389, 518, 882
SspDI GGCGCC 1 cut(s) 158
SstI GAGCTC 1 cut(s) 247
StyD4I CCNGG 3 cut(s) 228, 952, 1054
StyI CCWWGG 2 cut(s) 267, 1230
TaaI ACNGT 3 cut(s) 136, 556, 923
TaiI ACGT 1 cut(s) 1066
TaqI TCGA 2 cut(s) 75, 484
TasI AATT 8 cut(s) 190, 367, 473, 497, 531, 645, 719, 863
TatI WGTACW 2 cut(s) 126, 1214
TauI GCSGC 3 cut(s) 164, 391, 885
TfiI GAWTC 2 cut(s) 224, 536
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TscAI CASTG 3 cut(s) 288, 345, 1159
TseFI GTSAC 2 cut(s) 115, 975
TseI GCWGC 3 cut(s) 37, 335, 629
Tsp45I GTSAC 2 cut(s) 115, 975
TspDTI ATGAA 3 cut(s) 126, 276, 510
TspGWI ACGGA 2 cut(s) 804, 1179
TspRI CASTG 3 cut(s) 288, 345, 1159
XagI CCTNNNNNAGG 1 cut(s) 1041
XapI RAATTY 2 cut(s) 367, 473
XceI RCATGY 1 cut(s) 237
XcmI CCANNNNNNNNNTGG 1 cut(s) 236
XmnI GAANNNNTTC 1 cut(s) 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.