FvH4_7g25960

Putative tRNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0012158
Physical Location & Seq
Forward (+)
19746331 .. 19750941
4611 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25960.t1

Sequence Viewer

Length: 1194 bp
ATGGCAGCTAATGGAAGCATCGGCCTAAGCAGAAAGCAATTGATAGCTTCAGCACTATGCAAATACTTTGCCGTCGATCCTAAAACATTCCCAGAAGACATTGCTGAAGTTGAGATTGCAAGTTCCTACTTGACCATCTTGAAATCAGCCAAAAAGGAAGCCCCGGAGAAAGTGAATGATGAGGTGCTTAAATGGATAAACTATGCAGAGGGATTTCCTAGTGATCCCCAGGCATGCGATGGAGCTCTCAAAGAGTTGAATAAAACCTTGGTTGACAGGACTGTTCTTTTGGGCAATGGGTACACACCATCTGAAGCCGACGTTAGTGTGTTTCCTGCTGTGCATTCATCCGTGATTGGCCTTTCGAATTCAGACAGGAAAAAGTTTCAGCATGTATTTAGATGGGCGACTTACATCCAGCACAACGTGGATTTTGGAAACATGCTCGAGAAGATAGAGGTAGAGGAAGTTGAATTTGATTTCCAGAAGTTAGATGGTTTAGTGAAACCAAAAAGTGCAGCTAATCAGGAAGTAGATTCAAATGCAAAGAAGACAGTTCAAAGCACAAAAAGTGCTGACAAGCCAGAAGCAGGCTCAAGCACAAAGAAAAGTGAAACAGGGACAAAGACTGCAGGAGATAAGAAAGCTACTCAAGAGAAGAAAAAAGCACCTGAGGTAGAGGCAGTTGAGAAAGATAAAGAAGTTAGCGTCAGTTTGCTGAATATACAGGTTGGCCTCATTCGAAAAGCTTGGAAGCATCCATCTGCTGACAGTTTACTGGTTGAGGAGATAGATGTGGGAGATGCAAAAGTGAGGCAAGTGGTAAGCGGCCTGGCAAAGTTTTGCAGTCCAGAGGAATTGACAAACCGTCGTGTTGTGCTGATTACAAATGTGAAACCTGGGAAGTTACGTGACGTGATGTCAGAAGGACTGGTGCTGTGTGCTTCTAGTGCAGATCACACCCTTGTAGAGCCTTTGCTTCCACCGGAAGGGGCCAAAATCGGGGAACGTGTCTCATTTTCTGGGATTGATGGAAAGCCAGAAGATGTACTCAACCCAAAAAAGAAGCAGTTGGATAAGATCACACCGCATCTCTTCACTGATGACAAAGGTGTTGCTACATTCAAGGCTATTCCGTTCATGACATCTGCCGGGCCTTGCACATCATCCATTTGCAAAGGAAATGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C tRNA_bind

Protein Analysis

398

Amino Acids

43.11

Weight (kDa)

7.01

Isoelectric Point (pI)

32.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arc1p_N_like PF21972 81 - 142 1.2e-11 Nuclear-export cofactor Arc1p
tRNA_bind PF01588 242 - 337 2.8e-26 Putative tRNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012158)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40660
fragaria_vesca FvH4_7g25960
malus_domestica MD01G1168000.v1.1 MD07G1234000.v1.1
prunus_persica Prupe.2G264400_v2.0.a1
pyrus_communis pycom01g18100 pycom07g21180
rosa_chinensis RchiOBHm_Chr1g0372341
rosa_laevigata RLG00000026906
rosa_multiflora Rmu_sc0001755.1_g000007
rosa_roxburghii Rroxscaffold_4G00285030
rosa_rugosa Rorug01G0371300 Rorug01G0371300 Rorug01G0371300
rosa_samantha Rh1AG379700 Rh1BG343200 Rh1CG356700 Rh1DG374400
rosa_wichuraiana Rw1G033380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 828, 1088
AclWI GGATC 2 cut(s) 71, 218
AcsI RAATTY 2 cut(s) 367, 473
AcuI CTGAAG 3 cut(s) 33, 126, 333
AdeI CACNNNGTG 1 cut(s) 427
AfaI GTAC 2 cut(s) 302, 1050
AfiI CCNNNNNNNGG 3 cut(s) 590, 989, 1002
AflIII ACRYGT 1 cut(s) 1009
AgsI TTSAA 6 cut(s) 142, 259, 473, 540, 560, 1126
AhdI GACNNNNNGTC 2 cut(s) 867, 919
AjiI CACGTC 1 cut(s) 916
AjnI CCWGG 3 cut(s) 228, 831, 898
AjuI GAANNNNNNNTTGG 2 cut(s) 251, 283
AluBI AGCT 6 cut(s) 8, 47, 245, 521, 647, 749
AluI AGCT 6 cut(s) 8, 47, 245, 521, 647, 749
Alw21I GWGCWC 1 cut(s) 247
Alw26I GTCTC 1 cut(s) 1018
AlwI GGATC 2 cut(s) 71, 218
Ama87I CYCGRG 1 cut(s) 446
AoxI GGCC 6 cut(s) 22, 358, 733, 829, 993, 1154
ApeKI GCWGC 2 cut(s) 5, 518
ApoI RAATTY 2 cut(s) 367, 473
ArsI GACNNNNNNTTYG 3 cut(s) 271, 303, 1170
AspS9I GGNCC 2 cut(s) 993, 1154
AsuC2I CCSGG 2 cut(s) 164, 1153
AsuII TTCGAA 2 cut(s) 365, 742
AvaI CYCGRG 1 cut(s) 446
AxyI CCTNAGG 1 cut(s) 672
BanII GRGCYC 1 cut(s) 247
BbsI GAAGAC 2 cut(s) 102, 557
Bbv12I GWGCWC 1 cut(s) 247
BbvI GCAGC 2 cut(s) 17, 530
BccI CCATC 7 cut(s) 143, 233, 316, 396, 488, 769, 1025
BceAI ACGGC 1 cut(s) 56
BciT130I CCWGG 3 cut(s) 230, 833, 900
BcnI CCSGG 2 cut(s) 164, 1153
BcoDI GTCTC 1 cut(s) 1018
BfaI CTAG 2 cut(s) 219, 948
BfmI CTRYAG 1 cut(s) 630
BisI GCNGC 3 cut(s) 6, 519, 829
BlsI GCNGC 3 cut(s) 7, 520, 830
Bme1390I CCNGG 5 cut(s) 164, 230, 833, 900, 1153
BmeRI GACNNNNNGTC 2 cut(s) 867, 919
BmeT110I CYCGRG 1 cut(s) 446
BmgBI CACGTC 1 cut(s) 916
BmgT120I GGNCC 2 cut(s) 993, 1154
BmiI GGNNCC 1 cut(s) 994
BmrFI CCNGG 5 cut(s) 164, 230, 833, 900, 1153
BmsI GCATC 4 cut(s) 27, 766, 793, 1099
BpiI GAAGAC 2 cut(s) 102, 557
Bpu10I CCTNAGC 1 cut(s) 26
Bpu14I TTCGAA 2 cut(s) 365, 742
BpuEI CTTGAG 2 cut(s) 580, 636
BpuMI CCSGG 2 cut(s) 164, 1153
BsaAI YACGTR 1 cut(s) 911
BsaJI CCNNGG 4 cut(s) 162, 228, 267, 899
BsaWI WCCGGW 1 cut(s) 985
Bsc4I CCNNNNNNNGG 3 cut(s) 590, 989, 1002
Bse1I ACTGG 2 cut(s) 783, 936
Bse21I CCTNAGG 1 cut(s) 672
Bse3DI GCAATG 2 cut(s) 99, 301
BseBI CCWGG 3 cut(s) 230, 833, 900
BseDI CCNNGG 4 cut(s) 162, 228, 267, 899
BseGI GGATG 4 cut(s) 347, 414, 757, 1166
BseLI CCNNNNNNNGG 3 cut(s) 590, 989, 1002
BseMI GCAATG 2 cut(s) 99, 301
BseMII CTCAG 1 cut(s) 663
BseNI ACTGG 2 cut(s) 783, 936
BseRI GAGGAG 1 cut(s) 800
BseXI GCAGC 2 cut(s) 17, 530
BsgI GTGCAG 2 cut(s) 537, 972
BshFI GGCC 6 cut(s) 24, 360, 735, 831, 995, 1156
BsiHKAI GWGCWC 1 cut(s) 247
BsiHKCI CYCGRG 1 cut(s) 446
BsiSI CCGG 3 cut(s) 164, 986, 1152
BslFI GGGAC 1 cut(s) 634
BslI CCNNNNNNNGG 3 cut(s) 590, 989, 1002
BsmAI GTCTC 1 cut(s) 1018
BsmFI GGGAC 1 cut(s) 634
BsmI GAATGC 1 cut(s) 343
BsnI GGCC 6 cut(s) 24, 360, 735, 831, 995, 1156
BsoBI CYCGRG 1 cut(s) 446
Bsp119I TTCGAA 2 cut(s) 365, 742
Bsp1286I GDGCHC 1 cut(s) 247
Bsp143I GATC 4 cut(s) 76, 223, 955, 1080
BspACI CCGC 2 cut(s) 828, 1088
BspANI GGCC 6 cut(s) 24, 360, 735, 831, 995, 1156
BspCNI CTCAG 1 cut(s) 664
BspHI TCATGA 1 cut(s) 1140
BspLI GGNNCC 1 cut(s) 994
BspMAI CTGCAG 1 cut(s) 634
BspPI GGATC 2 cut(s) 71, 218
BspT104I TTCGAA 2 cut(s) 365, 742
BsrDI GCAATG 2 cut(s) 99, 301
BsrI ACTGG 2 cut(s) 783, 936
BssECI CCNNGG 4 cut(s) 162, 228, 267, 899
BssMI GATC 4 cut(s) 76, 223, 955, 1080
BssT1I CCWWGG 1 cut(s) 267
Bst2UI CCWGG 3 cut(s) 230, 833, 900
Bst4CI ACNGT 4 cut(s) 283, 556, 773, 869
Bst6I CTCTTC 1 cut(s) 1100
BstBAI YACGTR 1 cut(s) 911
BstBI TTCGAA 2 cut(s) 365, 742
BstC8I GCNNGC 2 cut(s) 235, 592
BstDEI CTNAG 2 cut(s) 26, 672
BstF5I GGATG 4 cut(s) 347, 414, 757, 1166
BstKTI GATC 4 cut(s) 79, 226, 958, 1083
BstMAI GTCTC 1 cut(s) 1018
BstMBI GATC 4 cut(s) 76, 223, 955, 1080
BstMWI GCNNNNNNNGC 1 cut(s) 950
BstNI CCWGG 3 cut(s) 230, 833, 900
BstNSI RCATGY 3 cut(s) 237, 395, 445
BstSCI CCNGG 5 cut(s) 162, 228, 831, 898, 1151
BstSFI CTRYAG 1 cut(s) 630
BstV1I GCAGC 2 cut(s) 17, 530
BstV2I GAAGAC 2 cut(s) 102, 557
Bsu36I CCTNAGG 1 cut(s) 672
BsuRI GGCC 6 cut(s) 24, 360, 735, 831, 995, 1156
BtgZI GCGATG 1 cut(s) 252
BtrI CACGTC 1 cut(s) 916
BtsCI GGATG 4 cut(s) 347, 414, 757, 1166
BtsIMutI CAGTG 1 cut(s) 1098
Cac8I GCNNGC 2 cut(s) 235, 592
CciI TCATGA 1 cut(s) 1140
Cfr13I GGNCC 2 cut(s) 993, 1154
CseI GACGC 1 cut(s) 697
Csp6I GTAC 2 cut(s) 301, 1049
CviAII CATG 4 cut(s) 234, 392, 442, 1141
CviQI GTAC 2 cut(s) 301, 1049
DdeI CTNAG 2 cut(s) 26, 672
DpnI GATC 4 cut(s) 78, 225, 957, 1082
DpnII GATC 4 cut(s) 76, 223, 955, 1080
DraIII CACNNNGTG 1 cut(s) 427
DriI GACNNNNNGTC 2 cut(s) 867, 919
Eam1104I CTCTTC 1 cut(s) 1100
Eam1105I GACNNNNNGTC 2 cut(s) 867, 919
EarI CTCTTC 1 cut(s) 1100
Ecl136II GAGCTC 1 cut(s) 245
Eco130I CCWWGG 1 cut(s) 267
Eco24I GRGCYC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 245
Eco57I CTGAAG 3 cut(s) 33, 126, 333
Eco81I CCTNAGG 1 cut(s) 672
Eco88I CYCGRG 1 cut(s) 446
EcoICRI GAGCTC 1 cut(s) 245
EcoRI GAATTC 1 cut(s) 367
EcoRII CCWGG 3 cut(s) 228, 831, 898
EcoT14I CCWWGG 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 247
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 4 cut(s) 237, 395, 445, 1144
FaiI YATR 7 cut(s) 58, 204, 235, 393, 443, 725, 1142
FaqI GGGAC 1 cut(s) 634
FatI CATG 4 cut(s) 233, 391, 441, 1140
Fnu4HI GCNGC 3 cut(s) 6, 519, 829
FokI GGATG 4 cut(s) 334, 401, 744, 1153
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 3 cut(s) 6, 519, 829
FspBI CTAG 2 cut(s) 219, 948
GluI GCNGC 3 cut(s) 6, 519, 829
HaeIII GGCC 6 cut(s) 24, 360, 735, 831, 995, 1156
HapII CCGG 3 cut(s) 164, 986, 1152
HgaI GACGC 1 cut(s) 697
Hin1II CATG 4 cut(s) 237, 395, 445, 1144
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 747
HinfI GANTC 1 cut(s) 536
HpaII CCGG 3 cut(s) 164, 986, 1152
Hpy166II GTNNAC 3 cut(s) 274, 303, 776
Hpy188I TCNGA 3 cut(s) 313, 373, 925
Hpy188III TCNNGA 7 cut(s) 139, 448, 484, 527, 653, 851, 1141
Hpy8I GTNNAC 3 cut(s) 274, 303, 776
Hpy99I CGWCG 3 cut(s) 77, 323, 873
HpyAV CCTTC 2 cut(s) 920, 983
HpyCH4III ACNGT 4 cut(s) 283, 556, 773, 869
HpyCH4IV ACGT 5 cut(s) 321, 426, 910, 915, 1009
HpyF10VI GCNNNNNNNGC 1 cut(s) 950
HpyF3I CTNAG 2 cut(s) 26, 672
HpySE526I ACGT 5 cut(s) 321, 426, 910, 915, 1009
Hsp92II CATG 4 cut(s) 237, 395, 445, 1144
Kzo9I GATC 4 cut(s) 76, 223, 955, 1080
LmnI GCTCC 1 cut(s) 242
Lsp1109I GCAGC 2 cut(s) 17, 530
LweI GCATC 4 cut(s) 27, 766, 793, 1099
MaeI CTAG 2 cut(s) 219, 948
MaeII ACGT 5 cut(s) 321, 426, 910, 915, 1009
MaeIII GTNAC 2 cut(s) 906, 911
MalI GATC 4 cut(s) 78, 225, 957, 1082
MboI GATC 4 cut(s) 76, 223, 955, 1080
MboII GAAGA 6 cut(s) 107, 463, 562, 670, 1055, 1087
MfeI CAATTG 1 cut(s) 38
MhlI GDGCHC 1 cut(s) 247
MluCI AATT 4 cut(s) 38, 367, 473, 857
MmeI TCCRAC 1 cut(s) 1053
MseI TTAA 1 cut(s) 189
MspI CCGG 3 cut(s) 164, 986, 1152
MspR9I CCNGG 5 cut(s) 164, 230, 833, 900, 1153
MunI CAATTG 1 cut(s) 38
Mva1269I GAATGC 1 cut(s) 343
MvaI CCWGG 3 cut(s) 230, 833, 900
MwoI GCNNNNNNNGC 1 cut(s) 950
NciI CCSGG 2 cut(s) 164, 1153
NdeII GATC 4 cut(s) 76, 223, 955, 1080
NlaIII CATG 4 cut(s) 237, 395, 445, 1144
NlaIV GGNNCC 1 cut(s) 994
NmuCI GTSAC 1 cut(s) 911
NspI RCATGY 3 cut(s) 237, 395, 445
NspV TTCGAA 2 cut(s) 365, 742
PaeI GCATGC 1 cut(s) 237
PaeR7I CTCGAG 1 cut(s) 446
PagI TCATGA 1 cut(s) 1140
PctI GAATGC 1 cut(s) 343
PfeI GAWTC 1 cut(s) 536
PkrI GCNGC 3 cut(s) 7, 520, 830
Ppu21I YACGTR 1 cut(s) 911
Psp124BI GAGCTC 1 cut(s) 247
Psp6I CCWGG 3 cut(s) 228, 831, 898
PspGI CCWGG 3 cut(s) 228, 831, 898
PspN4I GGNNCC 1 cut(s) 994
PspPI GGNCC 2 cut(s) 993, 1154
PstI CTGCAG 1 cut(s) 634
RsaI GTAC 2 cut(s) 302, 1050
RsaNI GTAC 2 cut(s) 301, 1049
SacI GAGCTC 1 cut(s) 247
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 3 cut(s) 6, 519, 829
Sau3AI GATC 4 cut(s) 76, 223, 955, 1080
Sau96I GGNCC 2 cut(s) 993, 1154
ScrFI CCNGG 5 cut(s) 164, 230, 833, 900, 1153
SduI GDGCHC 1 cut(s) 247
SfaNI GCATC 4 cut(s) 27, 766, 793, 1099
SfcI CTRYAG 1 cut(s) 630
Sfr274I CTCGAG 1 cut(s) 446
SfuI TTCGAA 2 cut(s) 365, 742
SlaI CTCGAG 1 cut(s) 446
SmlI CTYRAG 3 cut(s) 446, 595, 651
SmoI CTYRAG 3 cut(s) 446, 595, 651
SphI GCATGC 1 cut(s) 237
Sse9I AATT 4 cut(s) 38, 367, 473, 857
SsiI CCGC 2 cut(s) 828, 1088
SspMI CTAG 2 cut(s) 219, 948
SstI GAGCTC 1 cut(s) 247
StyD4I CCNGG 5 cut(s) 162, 228, 831, 898, 1151
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 4 cut(s) 283, 556, 773, 869
TaiI ACGT 5 cut(s) 324, 429, 913, 918, 1012
TaqI TCGA 4 cut(s) 75, 365, 447, 742
TasI AATT 4 cut(s) 38, 367, 473, 857
TatI WGTACW 1 cut(s) 1048
TauI GCSGC 1 cut(s) 831
TfiI GAWTC 1 cut(s) 536
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 1 cut(s) 1105
TseFI GTSAC 1 cut(s) 911
TseI GCWGC 2 cut(s) 5, 518
Tsp45I GTSAC 1 cut(s) 911
TspDTI ATGAA 2 cut(s) 336, 1129
TspGWI ACGGA 2 cut(s) 340, 1125
TspRI CASTG 1 cut(s) 1105
XapI RAATTY 2 cut(s) 367, 473
XceI RCATGY 3 cut(s) 237, 395, 445
XcmI CCANNNNNNNNNTGG 2 cut(s) 236, 491
XhoI CTCGAG 1 cut(s) 446
XspI CTAG 2 cut(s) 219, 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.