AT2G40760

rhodanese-like domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0012988
Physical Location & Seq
Forward (+)
17009951 .. 17012116
2166 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G40760.1

Sequence Viewer

Length: 1425 bp
ATGCTACGGTTTTACCACTGGAGATTTCCGCCGTCCCTCGCCGTCGTTAGAATGCTCTCATCACCACCCCCTCCTCACTCACACTCTCCATTCTCCGGCGGCGGCGTGAATTCGAACTCAGTCGGTGGAAACTCCAAACCCGAGTTACAATTCCCTCAATCCCAACCCCATAAACTCTCTTCCTCTCCTTCCTCTTCTCTGAAATCCACGGTGGCTTGTTCAAACGCCGGAGCTATACGGAGAAGCATGGCTACTGTTTCTCAGGCATTCTCAGAGAGAACAGAGTCTATTGATTCGGATTTAGGCTCGCTCGTTGTAGTTTCATTCTACAAATTCGCTGATTTTCCTGAGCATGCTGATTTCAGGAAGCCTCTCAAGGATCTCTGCGAAAAATTGCGTGTTTCAGGTGGAATCATTCTTGCACCAGAAGGGATAAATGGAAGTATCTGTGGGATTCGTGAATCAGTGGAGGAAGTGTTGGCATTTATACAGAGAGATGTCCGGTTAAATGGTTTGAGGCAAGTGGAGACACCTGTGAGTCCTGAGCAAGAAGCAATTCACCATGGACATAGTAGTAGCTCTCCTCTTGCAGCTGGTGAAGATGCTCCATTTAGATGGGATCATGTTAGGGTCAAACTTAAGAAAGAGATTGTTACGCTTGGAATTCCTAGTGTCTCGCCAATTGAAAGAGTTGGAACATATGTGAGTCCCGAAGAATGGAACGAACTGATCAGTGATCCAGAAACTGTAGTGATTGATGTGCGTAATACCTATGAGACTAGAATTGGGAAGTTTAAAGGAGCTGTGGATCCATGTACCACAGCTTTCAGAAATTTCCCATCTTGGGTGGAGAATCAATTTGCATTGAAGCAAGAAGGCAATGAAACGCAAGCAAAGGTGGAGAAAGAGGATTTTTCTGAAATCACCCATAAAGAAGACAAAGCTGAAAAACCGAAGACGCTGCCACGAATTGCTATGTATTGCACTGGAGGAATCAGGTGTGAGAAAGCTTCAAGCCTCCTTCTTAGCCAAGGTTTCGAAGAGGTATATCATCTGAAAGGTGGGATATTAAAGTACCTTGAAGAAGTCCCTAAAACCGAGAGCTTATGGGAAGGGGAATGCTTTGTGTTCGACAAACGTGTATCTGTGGAACATGGTCTAGCTCAAGGAACACATAAACTCTGCTATGGATGCAAGCAACCTATAAGTGATGAAGACATGGAAGCTCCAGAGTACGAGTATGGAGTTTCTTGCCCTTACTGTTACTCCAAGAAATCAGAAGAGGAGAAAGAAAGGGCAAGAGCAAGGCAGACACAGTTTGAGGAATGGGGTGTAATTGGTGGTCCGGACAAAGGTCGTCGACCCGCCACTAAACCTGATAGTCCAAGGAAGAAGATCAATGCAAAGCTTGGTTCTTCAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

474

Amino Acids

52.68

Weight (kDa)

6.39

Isoelectric Point (pI)

56.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0176_N PF17773 105 - 217 2.2e-19 UPF0176 acylphosphatase like domain
Rhodanese PF00581 236 - 361 7.5e-09 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40760
fragaria_vesca FvH4_7g26040
malus_domestica MD01G1168900.v1.1 MD07G1234800.v1.1
prunus_persica Prupe.2G265100_v2.0.a1
pyrus_communis pycom01g18170 pycom07g21240
rosa_chinensis RchiOBHm_Chr1g0372451
rosa_laevigata RLG00000026897
rosa_multiflora Rmu_sc0011709.1_g000010 Rmu_sc0019374.1_g000001
rosa_roxburghii Rroxscaffold_4G00284910
rosa_rugosa Rorug01G0372000
rosa_samantha Rh1AG380500 Rh1BG344100 Rh1CG357500 Rh1DG375300
rosa_wichuraiana Rw1G033460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1360
AccIII TCCGGA 1 cut(s) 1345
AciI CCGC 4 cut(s) 29, 99, 102, 1365
AclWI GGATC 5 cut(s) 387, 627, 731, 803, 816
AcsI RAATTY 4 cut(s) 109, 332, 663, 832
AfaI GTAC 3 cut(s) 817, 1076, 1235
AfiI CCNNNNNNNGG 4 cut(s) 95, 717, 844, 1352
AflII CTTAAG 1 cut(s) 638
AflIII ACRYGT 1 cut(s) 1138
AgsI TTSAA 6 cut(s) 222, 686, 868, 1014, 1082, 1419
Alw26I GTCTC 3 cut(s) 521, 679, 770
AlwI GGATC 5 cut(s) 387, 627, 731, 803, 816
AlwNI CAGNNNCTG 1 cut(s) 746
Ama87I CYCGRG 1 cut(s) 140
Aor13HI TCCGGA 1 cut(s) 1345
ApeKI GCWGC 2 cut(s) 590, 961
ApoI RAATTY 4 cut(s) 109, 332, 663, 832
Asp700I GAANNNNTTC 1 cut(s) 555
AspS9I GGNCC 1 cut(s) 1343
AsuHPI GGTGA 4 cut(s) 54, 551, 608, 916
AsuII TTCGAA 2 cut(s) 113, 1038
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 1 cut(s) 1343
BamHI GGATCC 1 cut(s) 808
BarI GAAGNNNNNNTAC 2 cut(s) 235, 267
BbsI GAAGAC 3 cut(s) 942, 962, 1221
BbvI GCAGC 2 cut(s) 602, 948
BccI CCATC 2 cut(s) 609, 847
BceAI ACGGC 2 cut(s) 16, 26
BcgI CGANNNNNNTGC 2 cut(s) 943, 977
BclI TGATCA 1 cut(s) 729
BcoDI GTCTC 3 cut(s) 521, 679, 770
BfaI CTAG 3 cut(s) 669, 780, 1160
BfmI CTRYAG 1 cut(s) 747
BfrI CTTAAG 1 cut(s) 638
BisI GCNGC 4 cut(s) 100, 103, 591, 962
BlsI GCNGC 4 cut(s) 101, 104, 592, 963
Bme18I GGWCC 1 cut(s) 1343
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 1343
BmiI GGNNCC 1 cut(s) 810
BmsI GCATC 2 cut(s) 592, 1181
BoxI GACNNNNGTC 1 cut(s) 1353
BpiI GAAGAC 3 cut(s) 942, 962, 1221
BpmI CTGGAG 3 cut(s) 40, 1008, 1212
Bpu10I CCTNAGC 2 cut(s) 348, 543
Bpu14I TTCGAA 2 cut(s) 113, 1038
BpuEI CTTGAG 2 cut(s) 359, 1149
BsaJI CCNNGG 4 cut(s) 207, 562, 1030, 1385
BsaWI WCCGGW 2 cut(s) 501, 1345
BsaXI ACNNNNNCTCC 4 cut(s) 461, 491, 1250, 1280
Bsc4I CCNNNNNNNGG 4 cut(s) 95, 717, 844, 1352
Bse1I ACTGG 2 cut(s) 23, 991
Bse3DI GCAATG 1 cut(s) 886
BseAI TCCGGA 1 cut(s) 1345
BseDI CCNNGG 4 cut(s) 207, 562, 1030, 1385
BseGI GGATG 1 cut(s) 1196
BseLI CCNNNNNNNGG 4 cut(s) 95, 717, 844, 1352
BseMI GCAATG 1 cut(s) 886
BseMII CTCAG 5 cut(s) 132, 275, 285, 339, 534
BseNI ACTGG 2 cut(s) 23, 991
BseRI GAGGAG 3 cut(s) 63, 573, 1298
BseXI GCAGC 2 cut(s) 602, 948
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 4 cut(s) 96, 228, 502, 1346
BslFI GGGAC 3 cut(s) 19, 693, 1073
BslI CCNNNNNNNGG 4 cut(s) 95, 717, 844, 1352
BsmAI GTCTC 3 cut(s) 521, 679, 770
BsmFI GGGAC 3 cut(s) 19, 693, 1073
BsmI GAATGC 3 cut(s) 57, 266, 1124
BsoBI CYCGRG 1 cut(s) 140
Bsp119I TTCGAA 2 cut(s) 113, 1038
Bsp13I TCCGGA 1 cut(s) 1345
Bsp143I GATC 6 cut(s) 379, 619, 729, 736, 808, 1395
Bsp19I CCATGG 1 cut(s) 562
BspACI CCGC 4 cut(s) 29, 99, 102, 1365
BspCNI CTCAG 5 cut(s) 131, 274, 284, 340, 535
BspEI TCCGGA 1 cut(s) 1345
BspLI GGNNCC 1 cut(s) 810
BspPI GGATC 5 cut(s) 387, 627, 731, 803, 816
BspT104I TTCGAA 2 cut(s) 113, 1038
BspTI CTTAAG 1 cut(s) 638
BsrDI GCAATG 1 cut(s) 886
BsrI ACTGG 2 cut(s) 23, 991
BssECI CCNNGG 4 cut(s) 207, 562, 1030, 1385
BssMI GATC 6 cut(s) 379, 619, 729, 736, 808, 1395
BssT1I CCWWGG 3 cut(s) 562, 1030, 1385
Bst4CI ACNGT 6 cut(s) 9, 211, 256, 748, 1262, 1317
Bst6I CTCTTC 4 cut(s) 184, 199, 1035, 1275
BstAFI CTTAAG 1 cut(s) 638
BstBI TTCGAA 2 cut(s) 113, 1038
BstC8I GCNNGC 4 cut(s) 308, 354, 891, 1196
BstDEI CTNAG 6 cut(s) 118, 261, 271, 348, 543, 1025
BstDSI CCRYGG 2 cut(s) 207, 562
BstF5I GGATG 1 cut(s) 1196
BstKTI GATC 6 cut(s) 382, 622, 732, 739, 811, 1398
BstMAI GTCTC 3 cut(s) 521, 679, 770
BstMBI GATC 6 cut(s) 379, 619, 729, 736, 808, 1395
BstMWI GCNNNNNNNGC 1 cut(s) 1191
BstNSI RCATGY 1 cut(s) 356
BstPAI GACNNNNGTC 1 cut(s) 1353
BstSFI CTRYAG 1 cut(s) 747
BstV1I GCAGC 2 cut(s) 602, 948
BstV2I GAAGAC 3 cut(s) 942, 962, 1221
BstX2I RGATCY 2 cut(s) 379, 808
BstXI CCANNNNNNTGG 1 cut(s) 615
BstYI RGATCY 2 cut(s) 379, 808
BtgI CCRYGG 2 cut(s) 207, 562
BtsCI GGATG 1 cut(s) 1196
BtsIMutI CAGTG 4 cut(s) 16, 471, 739, 984
Cac8I GCNNGC 4 cut(s) 308, 354, 891, 1196
CaiI CAGNNNCTG 1 cut(s) 746
Cfr13I GGNCC 1 cut(s) 1343
CseI GACGC 1 cut(s) 967
Csp6I GTAC 3 cut(s) 816, 1075, 1234
CviAII CATG 7 cut(s) 247, 353, 563, 623, 813, 1154, 1219
CviQI GTAC 3 cut(s) 816, 1075, 1234
DdeI CTNAG 6 cut(s) 118, 261, 271, 348, 543, 1025
DpnI GATC 6 cut(s) 381, 621, 731, 738, 810, 1397
DpnII GATC 6 cut(s) 379, 619, 729, 736, 808, 1395
DraI TTTAAA 1 cut(s) 796
Eam1104I CTCTTC 4 cut(s) 184, 199, 1035, 1275
EarI CTCTTC 4 cut(s) 184, 199, 1035, 1275
EciI GGCGGA 1 cut(s) 18
Eco130I CCWWGG 3 cut(s) 562, 1030, 1385
Eco47I GGWCC 1 cut(s) 1343
Eco88I CYCGRG 1 cut(s) 140
EcoRI GAATTC 2 cut(s) 109, 663
EcoT14I CCWWGG 3 cut(s) 562, 1030, 1385
ErhI CCWWGG 3 cut(s) 562, 1030, 1385
FaeI CATG 7 cut(s) 250, 356, 566, 626, 816, 1157, 1222
FaqI GGGAC 3 cut(s) 19, 693, 1073
FatI CATG 7 cut(s) 246, 352, 562, 622, 812, 1153, 1218
FauI CCCGC 1 cut(s) 1372
FauNDI CATATG 1 cut(s) 700
FbaI TGATCA 1 cut(s) 729
FblI GTMKAC 1 cut(s) 1360
Fnu4HI GCNGC 4 cut(s) 100, 103, 591, 962
FokI GGATG 1 cut(s) 1203
Fsp4HI GCNGC 4 cut(s) 100, 103, 591, 962
FspBI CTAG 3 cut(s) 669, 780, 1160
GluI GCNGC 4 cut(s) 100, 103, 591, 962
GsuI CTGGAG 3 cut(s) 40, 1008, 1212
HapII CCGG 4 cut(s) 96, 228, 502, 1346
HgaI GACGC 1 cut(s) 967
Hin1II CATG 7 cut(s) 250, 356, 566, 626, 816, 1157, 1222
HincII GTYRAC 1 cut(s) 1361
HindII GTYRAC 1 cut(s) 1361
HindIII AAGCTT 2 cut(s) 1008, 1406
HinfI GANTC 9 cut(s) 284, 293, 411, 454, 461, 538, 706, 853, 993
HpaII CCGG 4 cut(s) 96, 228, 502, 1346
HphI GGTGA 4 cut(s) 54, 551, 608, 916
Hpy166II GTNNAC 1 cut(s) 1361
Hpy188I TCNGA 7 cut(s) 201, 274, 298, 830, 919, 1056, 1279
Hpy188III TCNNGA 8 cut(s) 347, 364, 458, 542, 710, 740, 1229, 1346
Hpy8I GTNNAC 1 cut(s) 1361
Hpy99I CGWCG 2 cut(s) 47, 1362
HpyAV CCTTC 5 cut(s) 198, 422, 869, 1031, 1106
HpyCH4III ACNGT 6 cut(s) 9, 211, 256, 748, 1262, 1317
HpyCH4IV ACGT 1 cut(s) 1138
HpyCH4V TGCA 6 cut(s) 422, 590, 863, 984, 1194, 1403
HpyF10VI GCNNNNNNNGC 1 cut(s) 1191
HpyF3I CTNAG 6 cut(s) 118, 261, 271, 348, 543, 1025
HpySE526I ACGT 1 cut(s) 1138
Hsp92II CATG 7 cut(s) 250, 356, 566, 626, 816, 1157, 1222
Kpn2I TCCGGA 1 cut(s) 1345
Ksp22I TGATCA 1 cut(s) 729
Kzo9I GATC 6 cut(s) 379, 619, 729, 736, 808, 1395
LmnI GCTCC 4 cut(s) 230, 610, 800, 1231
Lsp1109I GCAGC 2 cut(s) 602, 948
LweI GCATC 2 cut(s) 592, 1181
MaeI CTAG 3 cut(s) 669, 780, 1160
MaeII ACGT 1 cut(s) 1138
MaeIII GTNAC 3 cut(s) 144, 652, 1262
MalI GATC 6 cut(s) 381, 621, 731, 738, 810, 1397
MboI GATC 6 cut(s) 379, 619, 729, 736, 808, 1395
MfeI CAATTG 1 cut(s) 681
MflI RGATCY 2 cut(s) 379, 808
MlyI GAGTC 3 cut(s) 293, 547, 715
MmeI TCCRAC 1 cut(s) 673
MroI TCCGGA 1 cut(s) 1345
MroXI GAANNNNTTC 1 cut(s) 555
MseI TTAA 4 cut(s) 506, 639, 795, 1070
MslI CAYNNNNRTG 1 cut(s) 613
MspA1I CMGCKG 1 cut(s) 593
MspCI CTTAAG 1 cut(s) 638
MspI CCGG 4 cut(s) 96, 228, 502, 1346
MunI CAATTG 1 cut(s) 681
Mva1269I GAATGC 3 cut(s) 57, 266, 1124
MwoI GCNNNNNNNGC 1 cut(s) 1191
NcoI CCATGG 1 cut(s) 562
NdeI CATATG 1 cut(s) 700
NdeII GATC 6 cut(s) 379, 619, 729, 736, 808, 1395
NlaIII CATG 7 cut(s) 250, 356, 566, 626, 816, 1157, 1222
NlaIV GGNNCC 1 cut(s) 810
NspI RCATGY 1 cut(s) 356
NspV TTCGAA 2 cut(s) 113, 1038
PaeI GCATGC 1 cut(s) 356
PctI GAATGC 3 cut(s) 57, 266, 1124
PdmI GAANNNNTTC 1 cut(s) 555
PfeI GAWTC 6 cut(s) 293, 411, 454, 461, 853, 993
PkrI GCNGC 4 cut(s) 101, 104, 592, 963
PleI GAGTC 3 cut(s) 292, 546, 714
PpsI GAGTC 3 cut(s) 292, 546, 714
PshAI GACNNNNGTC 1 cut(s) 1353
PspN4I GGNNCC 1 cut(s) 810
PspPI GGNCC 1 cut(s) 1343
PstNI CAGNNNCTG 1 cut(s) 746
PsuI RGATCY 2 cut(s) 379, 808
PvuII CAGCTG 1 cut(s) 593
RsaI GTAC 3 cut(s) 817, 1076, 1235
RsaNI GTAC 3 cut(s) 816, 1075, 1234
RseI CAYNNNNRTG 1 cut(s) 613
SalI GTCGAC 1 cut(s) 1359
SaqAI TTAA 4 cut(s) 506, 639, 795, 1070
SatI GCNGC 4 cut(s) 100, 103, 591, 962
Sau3AI GATC 6 cut(s) 379, 619, 729, 736, 808, 1395
Sau96I GGNCC 1 cut(s) 1343
SchI GAGTC 3 cut(s) 293, 547, 715
SfaNI GCATC 2 cut(s) 592, 1181
SfcI CTRYAG 1 cut(s) 747
SfuI TTCGAA 2 cut(s) 113, 1038
SinI GGWCC 1 cut(s) 1343
SmiMI CAYNNNNRTG 1 cut(s) 613
SmlI CTYRAG 3 cut(s) 374, 638, 1164
SmoI CTYRAG 3 cut(s) 374, 638, 1164
SphI GCATGC 1 cut(s) 356
SsiI CCGC 4 cut(s) 29, 99, 102, 1365
SspMI CTAG 3 cut(s) 669, 780, 1160
StyI CCWWGG 3 cut(s) 562, 1030, 1385
TaaI ACNGT 6 cut(s) 9, 211, 256, 748, 1262, 1317
TaiI ACGT 1 cut(s) 1141
TaqI TCGA 4 cut(s) 113, 1038, 1131, 1360
TauI GCSGC 2 cut(s) 102, 105
TfiI GAWTC 6 cut(s) 293, 411, 454, 461, 853, 993
Tru1I TTAA 4 cut(s) 506, 639, 795, 1070
Tru9I TTAA 4 cut(s) 506, 639, 795, 1070
TscAI CASTG 4 cut(s) 23, 471, 739, 991
TseI GCWGC 2 cut(s) 590, 961
TspDTI ATGAA 3 cut(s) 312, 897, 1227
TspGWI ACGGA 1 cut(s) 253
TspRI CASTG 4 cut(s) 23, 471, 739, 991
Vha464I CTTAAG 1 cut(s) 638
VpaK11BI GGWCC 1 cut(s) 1343
XapI RAATTY 4 cut(s) 109, 332, 663, 832
XceI RCATGY 1 cut(s) 356
XmiI GTMKAC 1 cut(s) 1360
XmnI GAANNNNTTC 1 cut(s) 555
XspI CTAG 3 cut(s) 669, 780, 1160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.