RLG00000026897

rhodanese-like domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0012988
Physical Location & Seq
Reverse (-)
6841262 .. 6843869
2608 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026897

Sequence Viewer

Length: 1398 bp
ATGCTAAGGTACAGAGCACCTCCATCACCCTCCCTGAGGATGCTATCTTCTTCTTCACCCTCACTGCACTCCCTAAACCCTAACCCTAAATCACACCCCGCCTCTTCAATTCCCGATTCCTCCCCCAATCGCCGTCTCCAACTCTTCTCTAATCCAATTCCAAACCCAATTGGGATTTTTAAACACTGTAACCCTCAAAGTATGACTCTTTCTAGATGCTTATCCGGGTCGACCAACCCAGTACCCATTTCGAGCTTACCCGAATCGGATTATCCGGATTCGAAAGTTCTGGTGGTGGTCTCCTTCTACAAGTTCGCTGATTTTCCTGACTGTGCTCGTTTGCGGCAACCCTTGAAGCAACTCTGCGAGGAATTGCGCGTTTCAGGTGGGATCATACTTGCACCGGAAGGGATCAATGGCAGCATATGCGGGAGCATAGAGTCGGTGGAGAAAGTTCTAGAGTTCATTCAAAGTGATGAGCGCCTAAGGGGGCTGAGAAGATTGGAGTCACCTGTGAGTCCGGAGGATGAAGCCATTCATCACGGACACAGTAGCAGTTCTCCTCTGGCAGCAGGGGAGGATGCACCCTTCCGATGGGATCATGTGAGGGTCAAGTTGAAGAAGGAGATTGTTACTCTTGGCATGCCTGCTGTATCACCTACTGAAAAAGTTGGGAAGTATGTGAGTCCAAGTGAATGGAATGAACTGATTAGTGATCCAGATACGGTTGTAATTGATGTGCGCAATAACTATGAAACTAGAATTGGGAAGTTTAAGGGAGCAGTTGATCCGTGTACTAAAGCATTCCGGGAGTTCCCGTCTTGGGTAGAGGATCAGTTCCAACATTCTGAACCGGACAATGGGCATACAGAAACAAATGGCAACAGTCCAGATGGAAGCACCAAAAATCAACCAGGGATTTCAAAACCAAAAACACCACCACGAGTTGCCATGTACTGTACTGGGGGCATTAGATGTGAGAAAGCTTCTAGTTATCTCCTTAGCAAAGGTTTTGAAGAGGTTTATCATCTAAAAGGTGGGATTCTGAAGTATCTTGAGGAAGTTCCAGAGAAAGAGAGCCTGTGGGAGGGAGAGTGCTTTGTCTTTGACAAGAGAGTCTCTGTTGAGCACGGTTTAGTACAAGGAAATCACAAATTATGCTATGGGTGTAAGCAACCAGTCAGTGATGCTGATATGGAGTCTCCTCAGTGGGAATACGGGGTTTCATGTCCCTATTGTTACTCGTCAAAAACCATCGAAGAAAAGGAAAGGGCAAGAGCTCGACAAAGGCAGTTTGAATCATGGGGTATCATTGGCGGTCCAGACAAGGGCTGTAAGCCAGGTACTATCAAACAGAGGCCTACTCAACTGTCAAATTCAATTTTGATGGAGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

466

Amino Acids

51.65

Weight (kDa)

6.65

Isoelectric Point (pI)

56.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0176_N PF17773 98 - 210 8.1e-22 UPF0176 acylphosphatase like domain
Rhodanese PF00581 230 - 353 2.3e-07 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40760
fragaria_vesca FvH4_7g26040
malus_domestica MD01G1168900.v1.1 MD07G1234800.v1.1
prunus_persica Prupe.2G265100_v2.0.a1
pyrus_communis pycom01g18170 pycom07g21240
rosa_chinensis RchiOBHm_Chr1g0372451
rosa_laevigata RLG00000026897
rosa_multiflora Rmu_sc0011709.1_g000010 Rmu_sc0019374.1_g000001
rosa_roxburghii Rroxscaffold_4G00284910
rosa_rugosa Rorug01G0372000
rosa_samantha Rh1AG380500 Rh1BG344100 Rh1CG357500 Rh1DG375300
rosa_wichuraiana Rw1G033460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1115
Acc16I TGCGCA 1 cut(s) 743
AccI GTMKAC 1 cut(s) 230
AccII CGCG 1 cut(s) 378
AccIII TCCGGA 2 cut(s) 274, 520
AciI CCGC 4 cut(s) 99, 343, 429, 1317
AclWI GGATC 6 cut(s) 398, 419, 606, 710, 782, 840
AcsI RAATTY 1 cut(s) 1375
AcuI CTGAAG 1 cut(s) 1067
AfaI GTAC 7 cut(s) 11, 243, 796, 956, 961, 1140, 1345
AfiI CCNNNNNNNGG 6 cut(s) 36, 594, 823, 860, 1087, 1328
AgsI TTSAA 8 cut(s) 108, 355, 470, 619, 924, 1016, 1298, 1380
AjnI CCWGG 2 cut(s) 913, 1339
AjuI GAANNNNNNNTTGG 2 cut(s) 747, 779
AluBI AGCT 3 cut(s) 255, 986, 1280
AluI AGCT 3 cut(s) 255, 986, 1280
Alw21I GWGCWC 4 cut(s) 19, 337, 1131, 1282
Alw26I GTCTC 5 cut(s) 140, 304, 1123, 1206, 1385
AlwI GGATC 6 cut(s) 398, 419, 606, 710, 782, 840
Aor13HI TCCGGA 2 cut(s) 274, 520
AoxI GGCC 1 cut(s) 1358
ApeKI GCWGC 2 cut(s) 420, 569
ApoI RAATTY 1 cut(s) 1375
Asp700I GAANNNNTTC 1 cut(s) 534
AspLEI GCGC 3 cut(s) 378, 483, 744
AspS9I GGNCC 1 cut(s) 1319
AsuC2I CCSGG 2 cut(s) 226, 809
AsuHPI GGTGA 4 cut(s) 18, 48, 501, 648
AsuII TTCGAA 1 cut(s) 281
AvaII GGWCC 1 cut(s) 1319
AxyI CCTNAGG 2 cut(s) 35, 485
BaeI ACNNNNGTAYC 2 cut(s) 714, 747
BanII GRGCYC 1 cut(s) 1282
BauI CACGAG 1 cut(s) 942
Bbv12I GWGCWC 4 cut(s) 19, 337, 1131, 1282
BbvI GCAGC 2 cut(s) 432, 581
BccI CCATC 5 cut(s) 31, 588, 887, 1262, 1381
BceAI ACGGC 1 cut(s) 117
BciT130I CCWGG 2 cut(s) 915, 1341
BcnI CCSGG 2 cut(s) 226, 809
BcoDI GTCTC 5 cut(s) 140, 304, 1123, 1206, 1385
BfaI CTAG 4 cut(s) 213, 458, 759, 990
BfoI RGCGCY 1 cut(s) 484
BisI GCNGC 3 cut(s) 344, 421, 570
BlsI GCNGC 3 cut(s) 345, 422, 571
Bme1390I CCNGG 4 cut(s) 226, 809, 915, 1341
Bme18I GGWCC 1 cut(s) 1319
BmgT120I GGNCC 1 cut(s) 1319
BmrFI CCNGG 4 cut(s) 226, 809, 915, 1341
BmrI ACTGGG 2 cut(s) 233, 972
BmsI GCATC 4 cut(s) 30, 206, 571, 1177
BmuI ACTGGG 2 cut(s) 233, 972
BplI GAGNNNNNCTC 2 cut(s) 1348, 1380
Bpu10I CCTNAGC 2 cut(s) 5, 1001
Bpu14I TTCGAA 1 cut(s) 281
BpuEI CTTGAG 1 cut(s) 1076
BpuMI CCSGG 2 cut(s) 226, 809
BsaBI GATNNNNATC 1 cut(s) 220
BsaI GGTCTC 1 cut(s) 304
BsaJI CCNNGG 1 cut(s) 914
BsaWI WCCGGW 4 cut(s) 274, 403, 520, 853
BsaXI ACNNNNNCTCC 4 cut(s) 803, 833, 1079, 1109
Bsc4I CCNNNNNNNGG 6 cut(s) 36, 594, 823, 860, 1087, 1328
Bse1I ACTGG 3 cut(s) 239, 967, 1178
Bse21I CCTNAGG 2 cut(s) 35, 485
Bse8I GATNNNNATC 1 cut(s) 220
BseAI TCCGGA 2 cut(s) 274, 520
BseBI CCWGG 2 cut(s) 915, 1341
BseDI CCNNGG 1 cut(s) 914
BseGI GGATG 3 cut(s) 45, 532, 586
BseJI GATNNNNATC 1 cut(s) 220
BseLI CCNNNNNNNGG 6 cut(s) 36, 594, 823, 860, 1087, 1328
BseMII CTCAG 3 cut(s) 26, 485, 1220
BseNI ACTGG 3 cut(s) 239, 967, 1178
BseRI GAGGAG 2 cut(s) 552, 1194
BseXI GCAGC 2 cut(s) 432, 581
BsgI GTGCAG 1 cut(s) 50
Bsh1236I CGCG 1 cut(s) 378
BshFI GGCC 1 cut(s) 1360
BsiHKAI GWGCWC 4 cut(s) 19, 337, 1131, 1282
BsiSI CCGG 6 cut(s) 225, 275, 404, 521, 808, 854
BslFI GGGAC 1 cut(s) 1215
BslI CCNNNNNNNGG 6 cut(s) 36, 594, 823, 860, 1087, 1328
BsmAI GTCTC 5 cut(s) 140, 304, 1123, 1206, 1385
BsmBI CGTCTC 1 cut(s) 140
BsmFI GGGAC 1 cut(s) 1215
BsmI GAATGC 1 cut(s) 803
BsnI GGCC 1 cut(s) 1360
Bso31I GGTCTC 1 cut(s) 304
Bsp119I TTCGAA 1 cut(s) 281
Bsp1286I GDGCHC 4 cut(s) 19, 337, 1131, 1282
Bsp13I TCCGGA 2 cut(s) 274, 520
Bsp143I GATC 6 cut(s) 390, 411, 598, 715, 787, 832
BspACI CCGC 4 cut(s) 99, 343, 429, 1317
BspANI GGCC 1 cut(s) 1360
BspCNI CTCAG 3 cut(s) 27, 486, 1219
BspEI TCCGGA 2 cut(s) 274, 520
BspFNI CGCG 1 cut(s) 378
BspPI GGATC 6 cut(s) 398, 419, 606, 710, 782, 840
BspT104I TTCGAA 1 cut(s) 281
BspTNI GGTCTC 1 cut(s) 304
BsrI ACTGG 3 cut(s) 239, 967, 1178
BssECI CCNNGG 1 cut(s) 914
BssMI GATC 6 cut(s) 390, 411, 598, 715, 787, 832
BssSI CACGAG 1 cut(s) 942
Bst2BI CACGAG 1 cut(s) 942
Bst2UI CCWGG 2 cut(s) 915, 1341
Bst4CI ACNGT 8 cut(s) 188, 332, 551, 727, 887, 959, 1133, 1371
Bst6I CTCTTC 3 cut(s) 109, 149, 1011
BstAPI GCANNNNNTGC 1 cut(s) 426
BstBI TTCGAA 1 cut(s) 281
BstC8I GCNNGC 2 cut(s) 644, 648
BstDEI CTNAG 6 cut(s) 5, 35, 485, 494, 1001, 1206
BstENI CCTNNNNNAGG 2 cut(s) 34, 1085
BstF5I GGATG 3 cut(s) 45, 532, 586
BstFNI CGCG 1 cut(s) 378
BstH2I RGCGCY 1 cut(s) 484
BstHHI GCGC 3 cut(s) 378, 483, 744
BstKTI GATC 6 cut(s) 393, 414, 601, 718, 790, 835
BstMAI GTCTC 5 cut(s) 140, 304, 1123, 1206, 1385
BstMBI GATC 6 cut(s) 390, 411, 598, 715, 787, 832
BstMWI GCNNNNNNNGC 1 cut(s) 426
BstNI CCWGG 2 cut(s) 915, 1341
BstNSI RCATGY 1 cut(s) 646
BstSCI CCNGG 4 cut(s) 224, 807, 913, 1339
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 2 cut(s) 432, 581
BstXI CCANNNNNNTGG 1 cut(s) 696
Bsu36I CCTNAGG 2 cut(s) 35, 485
BsuRI GGCC 1 cut(s) 1360
BtsCI GGATG 3 cut(s) 45, 532, 586
BtsI GCAGTG 1 cut(s) 62
BtsIMutI CAGTG 4 cut(s) 62, 184, 1189, 1214
Cac8I GCNNGC 2 cut(s) 644, 648
CfoI GCGC 3 cut(s) 378, 483, 744
Cfr13I GGNCC 1 cut(s) 1319
Csp6I GTAC 7 cut(s) 10, 242, 795, 955, 960, 1139, 1344
CviAII CATG 5 cut(s) 602, 643, 952, 1227, 1302
CviJI RGCY 9 cut(s) 255, 493, 533, 986, 1080, 1280, 1332, 1339, 1360
CviKI_1 RGCY 9 cut(s) 255, 493, 533, 986, 1080, 1280, 1332, 1339, 1360
CviQI GTAC 7 cut(s) 10, 242, 795, 955, 960, 1139, 1344
DdeI CTNAG 6 cut(s) 5, 35, 485, 494, 1001, 1206
DpnI GATC 6 cut(s) 392, 413, 600, 717, 789, 834
DpnII GATC 6 cut(s) 390, 411, 598, 715, 787, 832
DraI TTTAAA 1 cut(s) 181
DrdI GACNNNNNNGTC 1 cut(s) 1115
DseDI GACNNNNNNGTC 1 cut(s) 1115
Eam1104I CTCTTC 3 cut(s) 109, 149, 1011
EarI CTCTTC 3 cut(s) 109, 149, 1011
Ecl136II GAGCTC 1 cut(s) 1280
Eco147I AGGCCT 1 cut(s) 1360
Eco24I GRGCYC 1 cut(s) 1282
Eco31I GGTCTC 1 cut(s) 304
Eco47I GGWCC 1 cut(s) 1319
Eco53kI GAGCTC 1 cut(s) 1280
Eco57I CTGAAG 1 cut(s) 1067
Eco81I CCTNAGG 2 cut(s) 35, 485
EcoICRI GAGCTC 1 cut(s) 1280
EcoNI CCTNNNNNAGG 2 cut(s) 34, 1085
EcoRII CCWGG 2 cut(s) 913, 1339
EcoT38I GRGCYC 1 cut(s) 1282
Esp3I CGTCTC 1 cut(s) 140
FaeI CATG 5 cut(s) 605, 646, 955, 1230, 1305
FaqI GGGAC 1 cut(s) 1215
FatI CATG 5 cut(s) 601, 642, 951, 1226, 1301
FauI CCCGC 2 cut(s) 106, 422
FauNDI CATATG 1 cut(s) 425
FblI GTMKAC 1 cut(s) 230
Fnu4HI GCNGC 3 cut(s) 344, 421, 570
FokI GGATG 3 cut(s) 52, 539, 593
FriOI GRGCYC 1 cut(s) 1282
Fsp4HI GCNGC 3 cut(s) 344, 421, 570
FspBI CTAG 4 cut(s) 213, 458, 759, 990
FspI TGCGCA 1 cut(s) 743
GlaI GCGC 3 cut(s) 377, 482, 743
GluI GCNGC 3 cut(s) 344, 421, 570
HaeII RGCGCY 1 cut(s) 484
HaeIII GGCC 1 cut(s) 1360
HapII CCGG 6 cut(s) 225, 275, 404, 521, 808, 854
HhaI GCGC 3 cut(s) 378, 483, 744
Hin1II CATG 5 cut(s) 605, 646, 955, 1230, 1305
Hin6I GCGC 3 cut(s) 376, 481, 742
HinP1I GCGC 3 cut(s) 376, 481, 742
HincII GTYRAC 1 cut(s) 231
HindII GTYRAC 1 cut(s) 231
HindIII AAGCTT 1 cut(s) 984
HpaII CCGG 6 cut(s) 225, 275, 404, 521, 808, 854
HphI GGTGA 4 cut(s) 18, 48, 501, 648
Hpy166II GTNNAC 2 cut(s) 231, 795
Hpy188I TCNGA 4 cut(s) 268, 593, 850, 1047
Hpy8I GTNNAC 2 cut(s) 231, 795
HpyAV CCTTC 4 cut(s) 313, 401, 598, 616
HpyCH4III ACNGT 8 cut(s) 188, 332, 551, 727, 887, 959, 1133, 1371
HpyCH4V TGCA 3 cut(s) 67, 401, 584
HpyF10VI GCNNNNNNNGC 1 cut(s) 426
HpyF3I CTNAG 6 cut(s) 5, 35, 485, 494, 1001, 1206
Hsp92II CATG 5 cut(s) 605, 646, 955, 1230, 1305
HspAI GCGC 3 cut(s) 376, 481, 742
Kpn2I TCCGGA 2 cut(s) 274, 520
Kzo9I GATC 6 cut(s) 390, 411, 598, 715, 787, 832
LmnI GCTCC 2 cut(s) 432, 779
Lsp1109I GCAGC 2 cut(s) 432, 581
LweI GCATC 4 cut(s) 30, 206, 571, 1177
MaeI CTAG 4 cut(s) 213, 458, 759, 990
MaeIII GTNAC 4 cut(s) 188, 507, 631, 1238
MalI GATC 6 cut(s) 392, 413, 600, 717, 789, 834
MboI GATC 6 cut(s) 390, 411, 598, 715, 787, 832
MboII GAAGA 9 cut(s) 39, 42, 45, 96, 136, 510, 631, 1028, 1271
MfeI CAATTG 1 cut(s) 168
MhlI GDGCHC 4 cut(s) 19, 337, 1131, 1282
MluCI AATT 9 cut(s) 108, 156, 168, 371, 732, 762, 1154, 1375, 1380
MlyI GAGTC 7 cut(s) 199, 449, 515, 526, 694, 1125, 1208
MmeI TCCRAC 2 cut(s) 163, 865
MroI TCCGGA 2 cut(s) 274, 520
MroXI GAANNNNTTC 1 cut(s) 534
MseI TTAA 3 cut(s) 180, 774, 1396
MspI CCGG 6 cut(s) 225, 275, 404, 521, 808, 854
MspR9I CCNGG 4 cut(s) 226, 809, 915, 1341
MunI CAATTG 1 cut(s) 168
Mva1269I GAATGC 1 cut(s) 803
MvaI CCWGG 2 cut(s) 915, 1341
MvnI CGCG 1 cut(s) 378
MwoI GCNNNNNNNGC 1 cut(s) 426
NciI CCSGG 2 cut(s) 226, 809
NdeI CATATG 1 cut(s) 425
NdeII GATC 6 cut(s) 390, 411, 598, 715, 787, 832
NlaIII CATG 5 cut(s) 605, 646, 955, 1230, 1305
NmuCI GTSAC 1 cut(s) 507
NsbI TGCGCA 1 cut(s) 743
NspI RCATGY 1 cut(s) 646
NspV TTCGAA 1 cut(s) 281
PaeI GCATGC 1 cut(s) 646
PceI AGGCCT 1 cut(s) 1360
PctI GAATGC 1 cut(s) 803
PdmI GAANNNNTTC 1 cut(s) 534
PfeI GAWTC 5 cut(s) 116, 263, 278, 1042, 1298
PfoI TCCNGGA 1 cut(s) 807
PkrI GCNGC 3 cut(s) 345, 422, 571
PleI GAGTC 7 cut(s) 199, 448, 514, 525, 693, 1124, 1207
PpsI GAGTC 7 cut(s) 199, 448, 514, 525, 693, 1124, 1207
Psp124BI GAGCTC 1 cut(s) 1282
Psp6I CCWGG 2 cut(s) 913, 1339
PspGI CCWGG 2 cut(s) 913, 1339
PspPI GGNCC 1 cut(s) 1319
RsaI GTAC 7 cut(s) 11, 243, 796, 956, 961, 1140, 1345
RsaNI GTAC 7 cut(s) 10, 242, 795, 955, 960, 1139, 1344
SacI GAGCTC 1 cut(s) 1282
SalI GTCGAC 1 cut(s) 229
SaqAI TTAA 3 cut(s) 180, 774, 1396
SatI GCNGC 3 cut(s) 344, 421, 570
Sau3AI GATC 6 cut(s) 390, 411, 598, 715, 787, 832
Sau96I GGNCC 1 cut(s) 1319
SchI GAGTC 7 cut(s) 199, 449, 515, 526, 694, 1125, 1208
ScrFI CCNGG 4 cut(s) 226, 809, 915, 1341
SduI GDGCHC 4 cut(s) 19, 337, 1131, 1282
SfaNI GCATC 4 cut(s) 30, 206, 571, 1177
SfuI TTCGAA 1 cut(s) 281
SinI GGWCC 1 cut(s) 1319
SmlI CTYRAG 1 cut(s) 1055
SmoI CTYRAG 1 cut(s) 1055
SphI GCATGC 1 cut(s) 646
Sse9I AATT 9 cut(s) 108, 156, 168, 371, 732, 762, 1154, 1375, 1380
SseBI AGGCCT 1 cut(s) 1360
SsiI CCGC 4 cut(s) 99, 343, 429, 1317
SspMI CTAG 4 cut(s) 213, 458, 759, 990
SstI GAGCTC 1 cut(s) 1282
StuI AGGCCT 1 cut(s) 1360
StyD4I CCNGG 4 cut(s) 224, 807, 913, 1339
TaaI ACNGT 8 cut(s) 188, 332, 551, 727, 887, 959, 1133, 1371
TaqI TCGA 5 cut(s) 230, 251, 281, 1257, 1282
TasI AATT 9 cut(s) 108, 156, 168, 371, 732, 762, 1154, 1375, 1380
TatI WGTACW 4 cut(s) 794, 954, 959, 1138
TauI GCSGC 1 cut(s) 346
TfiI GAWTC 5 cut(s) 116, 263, 278, 1042, 1298
Tru1I TTAA 3 cut(s) 180, 774, 1396
Tru9I TTAA 3 cut(s) 180, 774, 1396
TscAI CASTG 4 cut(s) 69, 191, 1189, 1214
TseFI GTSAC 1 cut(s) 507
TseI GCWGC 2 cut(s) 420, 569
Tsp45I GTSAC 1 cut(s) 507
TspDTI ATGAA 6 cut(s) 454, 527, 543, 717, 768, 1215
TspGWI ACGGA 2 cut(s) 558, 780
TspRI CASTG 4 cut(s) 69, 191, 1189, 1214
VpaK11BI GGWCC 1 cut(s) 1319
XagI CCTNNNNNAGG 2 cut(s) 34, 1085
XapI RAATTY 1 cut(s) 1375
XbaI TCTAGA 2 cut(s) 212, 457
XceI RCATGY 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 230
XmnI GAANNNNTTC 1 cut(s) 534
XspI CTAG 4 cut(s) 213, 458, 759, 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.