pycom07g21240

rhodanese-like domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0012988
Physical Location & Seq
Forward (+)
23044421 .. 23046937
2517 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g21240.4

Sequence Viewer

Length: 1401 bp
ATGCGAACCTTGCCTCCCGCGGTCGACTCCATGCTAAGCTGGAGGTCACCTCCATCACTGTCGCTGAGGATGCTATGGTTACCTCCACTGCACTCCGTAAACCCTAACCCTAGATTGCATCCCGCCTCTTCAGTTTCGCCGCCCTCCAATCGAGATCCTCAGTCGTTTCTCAACCCTAGGATTCCGGTCTCCGATTCTCGCGGCGTCCCTCAAAGCATGACGCTTTCCAGGTGCTTCTCCGGGCCCACCAGTCCGGTCCCGATTCCGAGCTCGCCCGAGTCGGATAACTCGGATTCGCAGATTCTCGTCGTGGTCTCCTTCTACAAGTTCGCTGATTTTGCCGACCATGCTTCTCTGCGGCAACCATTGAAGCAGCTTTGCGAAGAACTGCGTGTTTCAGGCGGTATCATACTTGCCCGGGAAGGGATAAATGGGAGCATTTGTGGGACAAGAGAATCGGTGGATAAAGTTCTTGAGTTCATCCAAAATGATAACTGCCTTGAGGGACTAAGAGGATTGGAGTCACCTGTGAGTCCTGAGGAGGAAGCTATTCATCACGGGCACACTAGCAGCTCTCCTCTGGCAGCAGGGGAAGACGCGCCCTTCAGATGGGATCATGTGAGGGTCAAGTTGAAGAAGGAGATTGTTAGTCTTGGAATGCCTGATGTATCGCCAATTGAAAAGGTCGGAAAGTATGTGAGTCCACGGGATTGGAATGCATTGATTAGTGATCCAGATACGGTGGTTATTGATGTGCGCAATAATTACGAAACTAGAATAGGGAAGTTCAAGGGAGCAGTTGATCCGTGCACTAAAGCATTCCGAGAGTTCCCATCTTGGGTAGACGATCTGTTTCAACCTTCAGAATCAGAGAATGGTCATTTGGAGGTGAAACTCAACGGTCCAAATGAAAGCATCAACAGTCAGCTCGAGATATCAAAACCAAAAACACCTCCGAAAGTTGCCATGTACTGTACTGGGGGAATTAGATGTGAGAAAGCTTCAAGTTTTCTCCTCAGCAAAGGCTTCGAAGAGGTTTATCATCTAGAAGGCGGAATTCTGAAGTATCTTGAGGAAGTTCCACGGACGGAGAGCCTGTGGGAGGGAGAGTGCTTTGTCTTTGACAAGAGAGTCTCGGTTGAGCATGGGTTAGCACAGGGAACTCACAAATTATGCTACGGGTGTAAGCAACCGGTCAGTGATGCAGATATGGAGTCTCCTCAGTGGGAATACGGAGTTACTTGTCCGTACTGTTTCTCGTCAAAATCTGATGAAGAGAAGGATAGGGCAAGAGCTCGACAAAGGCAATTTGAGACATGGGGGATCATCGGTGGTCCGGACAACGGACGCAAACCGGATGGTACCAAGCACAGCTCTACTCATCTCTCAAATTCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

467

Amino Acids

51.65

Weight (kDa)

5.78

Isoelectric Point (pI)

53.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40760
fragaria_vesca FvH4_7g26040
malus_domestica MD01G1168900.v1.1 MD07G1234800.v1.1
prunus_persica Prupe.2G265100_v2.0.a1
pyrus_communis pycom01g18170 pycom07g21240
rosa_chinensis RchiOBHm_Chr1g0372451
rosa_laevigata RLG00000026897
rosa_multiflora Rmu_sc0011709.1_g000010 Rmu_sc0019374.1_g000001
rosa_roxburghii Rroxscaffold_4G00284910
rosa_rugosa Rorug01G0372000
rosa_samantha Rh1AG380500 Rh1BG344100 Rh1CG357500 Rh1DG375300
rosa_wichuraiana Rw1G033460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1130
Acc16I TGCGCA 1 cut(s) 758
Acc65I GGTACC 1 cut(s) 1361
AccB1I GGYRCC 1 cut(s) 1361
AccI GTMKAC 2 cut(s) 24, 843
AccII CGCG 3 cut(s) 20, 201, 599
AccIII TCCGGA 1 cut(s) 1336
AciI CCGC 8 cut(s) 18, 20, 123, 140, 201, 358, 402, 1053
AclWI GGATC 5 cut(s) 149, 621, 725, 797, 1331
AcsI RAATTY 2 cut(s) 1056, 1390
AcuI CTGAAG 4 cut(s) 114, 589, 846, 1082
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 4 cut(s) 971, 976, 1250, 1363
AfiI CCNNNNNNNGG 5 cut(s) 423, 609, 838, 1102, 1343
AgeI ACCGGT 1 cut(s) 1192
AgsI TTSAA 6 cut(s) 370, 634, 680, 790, 857, 1005
AjnI CCWGG 1 cut(s) 227
AjuI GAANNNNNNNTTGG 2 cut(s) 866, 898
AloI GAACNNNNNNTCC 1 cut(s) 30
AluBI AGCT 9 cut(s) 39, 270, 376, 548, 573, 928, 1001, 1295, 1374
AluI AGCT 9 cut(s) 39, 270, 376, 548, 573, 928, 1001, 1295, 1374
Alw21I GWGCWC 3 cut(s) 272, 812, 1297
Alw26I GTCTC 5 cut(s) 193, 319, 1138, 1221, 1307
Alw44I GTGCAC 1 cut(s) 808
AlwI GGATC 5 cut(s) 149, 621, 725, 797, 1331
Ama87I CYCGRG 3 cut(s) 275, 417, 929
Aor13HI TCCGGA 1 cut(s) 1336
AoxI GGCC 1 cut(s) 242
ApaI GGGCCC 1 cut(s) 246
ApaLI GTGCAC 1 cut(s) 808
ApeKI GCWGC 3 cut(s) 373, 570, 584
ApoI RAATTY 2 cut(s) 1056, 1390
AsiGI ACCGGT 1 cut(s) 1192
Asp700I GAANNNNTTC 1 cut(s) 549
Asp718I GGTACC 1 cut(s) 1361
AspA2I CCTAGG 1 cut(s) 176
AspLEI GCGC 2 cut(s) 601, 759
AspS9I GGNCC 5 cut(s) 242, 243, 256, 902, 1334
AsuC2I CCSGG 3 cut(s) 241, 418, 419
AsuHPI GGTGA 3 cut(s) 39, 516, 901
AsuII TTCGAA 1 cut(s) 1029
AvaI CYCGRG 3 cut(s) 275, 417, 929
AvaII GGWCC 3 cut(s) 256, 902, 1334
AvrII CCTAGG 1 cut(s) 176
AxyI CCTNAGG 1 cut(s) 537
BaeGI GKGCMC 3 cut(s) 246, 564, 812
BaeI ACNNNNGTAYC 2 cut(s) 729, 762
BanI GGYRCC 1 cut(s) 1361
BanII GRGCYC 3 cut(s) 246, 272, 1297
BbsI GAAGAC 1 cut(s) 600
Bbv12I GWGCWC 3 cut(s) 272, 812, 1297
BbvCI CCTCAGC 2 cut(s) 65, 1016
BbvI GCAGC 3 cut(s) 385, 582, 596
BccI CCATC 4 cut(s) 61, 603, 841, 1352
BcgI CGANNNNNNTGC 2 cut(s) 1009, 1043
BciT130I CCWGG 1 cut(s) 229
BcnI CCSGG 3 cut(s) 241, 418, 419
BcoDI GTCTC 5 cut(s) 193, 319, 1138, 1221, 1307
BfaI CTAG 5 cut(s) 111, 177, 567, 774, 1046
BisI GCNGC 6 cut(s) 140, 202, 359, 374, 571, 585
BlnI CCTAGG 1 cut(s) 176
BlpI GCTNAGC 1 cut(s) 35
BlsI GCNGC 6 cut(s) 141, 203, 360, 375, 572, 586
Bme1390I CCNGG 4 cut(s) 229, 241, 418, 419
Bme18I GGWCC 3 cut(s) 256, 902, 1334
BmeT110I CYCGRG 3 cut(s) 275, 417, 929
BmgT120I GGNCC 5 cut(s) 242, 243, 256, 902, 1334
BmiI GGNNCC 3 cut(s) 244, 258, 1363
BmrFI CCNGG 4 cut(s) 229, 241, 418, 419
BmrI ACTGGG 1 cut(s) 987
BmsI GCATC 4 cut(s) 60, 127, 924, 1192
BmuI ACTGGG 1 cut(s) 987
BpiI GAAGAC 1 cut(s) 600
BplI GAGNNNNNCTC 2 cut(s) 34, 66
BpmI CTGGAG 1 cut(s) 61
Bpu10I CCTNAGC 2 cut(s) 65, 1016
Bpu1102I GCTNAGC 1 cut(s) 35
Bpu14I TTCGAA 1 cut(s) 1029
BpuEI CTTGAG 3 cut(s) 494, 521, 1091
BpuMI CCSGG 3 cut(s) 241, 418, 419
BsaHI GRCGYC 1 cut(s) 204
BsaI GGTCTC 2 cut(s) 193, 319
BsaJI CCNNGG 5 cut(s) 18, 176, 417, 704, 1082
BsaWI WCCGGW 5 cut(s) 184, 253, 1192, 1336, 1354
BsaXI ACNNNNNCTCC 7 cut(s) 28, 427, 457, 1082, 1094, 1112, 1124
Bsc4I CCNNNNNNNGG 5 cut(s) 423, 609, 838, 1102, 1343
Bse118I RCCGGY 1 cut(s) 1192
Bse1I ACTGG 2 cut(s) 249, 982
Bse21I CCTNAGG 1 cut(s) 537
BseAI TCCGGA 1 cut(s) 1336
BseBI CCWGG 1 cut(s) 229
BseDI CCNNGG 5 cut(s) 18, 176, 417, 704, 1082
BseGI GGATG 4 cut(s) 75, 118, 480, 1363
BseLI CCNNNNNNNGG 5 cut(s) 423, 609, 838, 1102, 1343
BseMII CTCAG 5 cut(s) 56, 173, 528, 1030, 1235
BseNI ACTGG 2 cut(s) 249, 982
BseRI GAGGAG 4 cut(s) 554, 567, 1004, 1209
BseSI GKGCMC 3 cut(s) 246, 564, 812
BseXI GCAGC 3 cut(s) 385, 582, 596
BsgI GTGCAG 1 cut(s) 74
Bsh1236I CGCG 3 cut(s) 20, 201, 599
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 1 cut(s) 244
BshNI GGYRCC 1 cut(s) 1361
BshTI ACCGGT 1 cut(s) 1192
BsiEI CGRYCG 1 cut(s) 24
BsiHKAI GWGCWC 3 cut(s) 272, 812, 1297
BsiHKCI CYCGRG 3 cut(s) 275, 417, 929
BsiSI CCGG 7 cut(s) 185, 240, 254, 418, 1193, 1337, 1355
BslFI GGGAC 4 cut(s) 191, 242, 460, 519
BslI CCNNNNNNNGG 5 cut(s) 423, 609, 838, 1102, 1343
BsmAI GTCTC 5 cut(s) 193, 319, 1138, 1221, 1307
BsmFI GGGAC 4 cut(s) 191, 242, 460, 519
BsmI GAATGC 3 cut(s) 663, 721, 818
BsnI GGCC 1 cut(s) 244
Bso31I GGTCTC 2 cut(s) 193, 319
BsoBI CYCGRG 3 cut(s) 275, 417, 929
Bsp119I TTCGAA 1 cut(s) 1029
Bsp120I GGGCCC 1 cut(s) 242
Bsp1286I GDGCHC 5 cut(s) 246, 272, 564, 812, 1297
Bsp13I TCCGGA 1 cut(s) 1336
Bsp143I GATC 6 cut(s) 154, 613, 730, 802, 847, 1323
Bsp1720I GCTNAGC 1 cut(s) 35
BspACI CCGC 8 cut(s) 18, 20, 123, 140, 201, 358, 402, 1053
BspANI GGCC 1 cut(s) 244
BspCNI CTCAG 5 cut(s) 57, 172, 529, 1029, 1234
BspEI TCCGGA 1 cut(s) 1336
BspFNI CGCG 3 cut(s) 20, 201, 599
BspLI GGNNCC 3 cut(s) 244, 258, 1363
BspPI GGATC 5 cut(s) 149, 621, 725, 797, 1331
BspT104I TTCGAA 1 cut(s) 1029
BspT107I GGYRCC 1 cut(s) 1361
BspTNI GGTCTC 2 cut(s) 193, 319
BsrFI RCCGGY 1 cut(s) 1192
BsrI ACTGG 2 cut(s) 249, 982
BssAI RCCGGY 1 cut(s) 1192
BssECI CCNNGG 5 cut(s) 18, 176, 417, 704, 1082
BssMI GATC 6 cut(s) 154, 613, 730, 802, 847, 1323
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 176
Bst2UI CCWGG 1 cut(s) 229
Bst4CI ACNGT 6 cut(s) 60, 742, 902, 923, 974, 1253
Bst6I CTCTTC 3 cut(s) 133, 1026, 1269
BstACI GRCGYC 1 cut(s) 204
BstBI TTCGAA 1 cut(s) 1029
BstC8I GCNNGC 1 cut(s) 272
BstDEI CTNAG 7 cut(s) 35, 65, 159, 509, 537, 1016, 1221
BstDSI CCRYGG 3 cut(s) 18, 704, 1082
BstEII GGTNACC 2 cut(s) 45, 78
BstENI CCTNNNNNAGG 1 cut(s) 1100
BstF5I GGATG 4 cut(s) 75, 118, 480, 1363
BstFNI CGCG 3 cut(s) 20, 201, 599
BstHHI GCGC 2 cut(s) 601, 759
BstKTI GATC 6 cut(s) 157, 616, 733, 805, 850, 1326
BstMAI GTCTC 5 cut(s) 193, 319, 1138, 1221, 1307
BstMBI GATC 6 cut(s) 154, 613, 730, 802, 847, 1323
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 4 cut(s) 10, 70, 338, 347
BstNI CCWGG 1 cut(s) 229
BstPI GGTNACC 2 cut(s) 45, 78
BstSCI CCNGG 4 cut(s) 227, 239, 416, 417
BstSLI GKGCMC 3 cut(s) 246, 564, 812
BstUI CGCG 3 cut(s) 20, 201, 599
BstV1I GCAGC 3 cut(s) 385, 582, 596
BstV2I GAAGAC 1 cut(s) 600
BstX2I RGATCY 1 cut(s) 154
BstXI CCANNNNNNTGG 1 cut(s) 711
BstYI RGATCY 1 cut(s) 154
Bsu36I CCTNAGG 1 cut(s) 537
BsuRI GGCC 1 cut(s) 244
BtgI CCRYGG 3 cut(s) 18, 704, 1082
BtsCI GGATG 4 cut(s) 75, 118, 480, 1363
BtsI GCAGTG 1 cut(s) 86
BtsIMutI CAGTG 4 cut(s) 56, 86, 1204, 1229
Cac8I GCNNGC 1 cut(s) 272
CfoI GCGC 2 cut(s) 601, 759
Cfr10I RCCGGY 1 cut(s) 1192
Cfr13I GGNCC 5 cut(s) 242, 243, 256, 902, 1334
Cfr42I CCGCGG 1 cut(s) 21
Cfr9I CCCGGG 1 cut(s) 417
CseI GACGC 4 cut(s) 193, 229, 605, 1356
Csp6I GTAC 4 cut(s) 970, 975, 1249, 1362
CspAI ACCGGT 1 cut(s) 1192
CviAII CATG 7 cut(s) 31, 217, 347, 617, 967, 1145, 1317
CviQI GTAC 4 cut(s) 970, 975, 1249, 1362
DdeI CTNAG 7 cut(s) 35, 65, 159, 509, 537, 1016, 1221
DpnI GATC 6 cut(s) 156, 615, 732, 804, 849, 1325
DpnII GATC 6 cut(s) 154, 613, 730, 802, 847, 1323
DrdI GACNNNNNNGTC 1 cut(s) 1130
DseDI GACNNNNNNGTC 1 cut(s) 1130
Eam1104I CTCTTC 3 cut(s) 133, 1026, 1269
EarI CTCTTC 3 cut(s) 133, 1026, 1269
EciI GGCGGA 1 cut(s) 1068
Ecl136II GAGCTC 2 cut(s) 270, 1295
Eco130I CCWWGG 1 cut(s) 176
Eco24I GRGCYC 3 cut(s) 246, 272, 1297
Eco31I GGTCTC 2 cut(s) 193, 319
Eco32I GATATC 1 cut(s) 936
Eco47I GGWCC 3 cut(s) 256, 902, 1334
Eco53kI GAGCTC 2 cut(s) 270, 1295
Eco57I CTGAAG 4 cut(s) 114, 589, 846, 1082
Eco81I CCTNAGG 1 cut(s) 537
Eco88I CYCGRG 3 cut(s) 275, 417, 929
Eco91I GGTNACC 2 cut(s) 45, 78
EcoICRI GAGCTC 2 cut(s) 270, 1295
EcoNI CCTNNNNNAGG 1 cut(s) 1100
EcoO65I GGTNACC 2 cut(s) 45, 78
EcoRI GAATTC 1 cut(s) 1056
EcoRII CCWGG 1 cut(s) 227
EcoRV GATATC 1 cut(s) 936
EcoT14I CCWWGG 1 cut(s) 176
EcoT22I ATGCAT 1 cut(s) 721
EcoT38I GRGCYC 3 cut(s) 246, 272, 1297
ErhI CCWWGG 1 cut(s) 176
FaeI CATG 7 cut(s) 34, 220, 350, 620, 970, 1148, 1320
FaqI GGGAC 4 cut(s) 191, 242, 460, 519
FatI CATG 7 cut(s) 30, 216, 346, 616, 966, 1144, 1316
FauI CCCGC 2 cut(s) 25, 130
FblI GTMKAC 2 cut(s) 24, 843
Fnu4HI GCNGC 6 cut(s) 140, 202, 359, 374, 571, 585
FokI GGATG 4 cut(s) 82, 105, 467, 1370
FriOI GRGCYC 3 cut(s) 246, 272, 1297
Fsp4HI GCNGC 6 cut(s) 140, 202, 359, 374, 571, 585
FspBI CTAG 5 cut(s) 111, 177, 567, 774, 1046
FspI TGCGCA 1 cut(s) 758
GlaI GCGC 2 cut(s) 600, 758
GluI GCNGC 6 cut(s) 140, 202, 359, 374, 571, 585
GsuI CTGGAG 1 cut(s) 61
HaeIII GGCC 1 cut(s) 244
HapII CCGG 7 cut(s) 185, 240, 254, 418, 1193, 1337, 1355
HgaI GACGC 4 cut(s) 193, 229, 605, 1356
HhaI GCGC 2 cut(s) 601, 759
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 7 cut(s) 34, 220, 350, 620, 970, 1148, 1320
Hin6I GCGC 2 cut(s) 599, 757
HinP1I GCGC 2 cut(s) 599, 757
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HindIII AAGCTT 1 cut(s) 999
HpaII CCGG 7 cut(s) 185, 240, 254, 418, 1193, 1337, 1355
HphI GGTGA 3 cut(s) 39, 516, 901
Hpy166II GTNNAC 5 cut(s) 25, 100, 704, 810, 844
Hpy188III TCNNGA 9 cut(s) 152, 259, 473, 536, 734, 931, 1046, 1070, 1337
Hpy8I GTNNAC 5 cut(s) 25, 100, 704, 810, 844
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 7 cut(s) 328, 416, 613, 631, 870, 1043, 1273
HpyCH4III ACNGT 6 cut(s) 60, 742, 902, 923, 974, 1253
HpyCH4V TGCA 5 cut(s) 91, 118, 719, 810, 1205
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 70, 338, 347
HpyF3I CTNAG 7 cut(s) 35, 65, 159, 509, 537, 1016, 1221
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 7 cut(s) 34, 220, 350, 620, 970, 1148, 1320
HspAI GCGC 2 cut(s) 599, 757
Kpn2I TCCGGA 1 cut(s) 1336
KpnI GGTACC 1 cut(s) 1365
KspI CCGCGG 1 cut(s) 21
Kzo9I GATC 6 cut(s) 154, 613, 730, 802, 847, 1323
LmnI GCTCC 2 cut(s) 435, 794
Lsp1109I GCAGC 3 cut(s) 385, 582, 596
LweI GCATC 4 cut(s) 60, 127, 924, 1192
MaeI CTAG 5 cut(s) 111, 177, 567, 774, 1046
MaeIII GTNAC 4 cut(s) 45, 78, 522, 1237
MalI GATC 6 cut(s) 156, 615, 732, 804, 849, 1325
MboI GATC 6 cut(s) 154, 613, 730, 802, 847, 1323
MboII GAAGA 6 cut(s) 120, 395, 605, 646, 1043, 1286
MfeI CAATTG 1 cut(s) 675
MflI RGATCY 1 cut(s) 154
MhlI GDGCHC 5 cut(s) 246, 272, 564, 812, 1297
MluCI AATT 7 cut(s) 675, 763, 984, 1056, 1169, 1307, 1390
MlyI GAGTC 7 cut(s) 20, 287, 530, 541, 709, 1140, 1223
MmeI TCCRAC 2 cut(s) 261, 667
Mph1103I ATGCAT 1 cut(s) 721
MroI TCCGGA 1 cut(s) 1336
MroXI GAANNNNTTC 1 cut(s) 549
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 7 cut(s) 185, 240, 254, 418, 1193, 1337, 1355
MspR9I CCNGG 4 cut(s) 229, 241, 418, 419
MunI CAATTG 1 cut(s) 675
Mva1269I GAATGC 3 cut(s) 663, 721, 818
MvaI CCWGG 1 cut(s) 229
MvnI CGCG 3 cut(s) 20, 201, 599
MwoI GCNNNNNNNGC 4 cut(s) 10, 70, 338, 347
NciI CCSGG 3 cut(s) 241, 418, 419
NdeII GATC 6 cut(s) 154, 613, 730, 802, 847, 1323
NlaIII CATG 7 cut(s) 34, 220, 350, 620, 970, 1148, 1320
NlaIV GGNNCC 3 cut(s) 244, 258, 1363
NmuCI GTSAC 2 cut(s) 45, 522
NsbI TGCGCA 1 cut(s) 758
NsiI ATGCAT 1 cut(s) 721
NspV TTCGAA 1 cut(s) 1029
PaeR7I CTCGAG 1 cut(s) 929
PctI GAATGC 3 cut(s) 663, 721, 818
PdmI GAANNNNTTC 1 cut(s) 549
PfeI GAWTC 7 cut(s) 181, 194, 262, 293, 301, 455, 866
PinAI ACCGGT 1 cut(s) 1192
PkrI GCNGC 6 cut(s) 141, 203, 360, 375, 572, 586
PleI GAGTC 7 cut(s) 20, 286, 529, 540, 708, 1139, 1222
PpsI GAGTC 7 cut(s) 20, 286, 529, 540, 708, 1139, 1222
Psp124BI GAGCTC 2 cut(s) 272, 1297
Psp6I CCWGG 1 cut(s) 227
PspEI GGTNACC 2 cut(s) 45, 78
PspGI CCWGG 1 cut(s) 227
PspN4I GGNNCC 3 cut(s) 244, 258, 1363
PspOMI GGGCCC 1 cut(s) 242
PspPI GGNCC 5 cut(s) 242, 243, 256, 902, 1334
PsuI RGATCY 1 cut(s) 154
RsaI GTAC 4 cut(s) 971, 976, 1250, 1363
RsaNI GTAC 4 cut(s) 970, 975, 1249, 1362
SacI GAGCTC 2 cut(s) 272, 1297
SacII CCGCGG 1 cut(s) 21
SalI GTCGAC 1 cut(s) 23
SatI GCNGC 6 cut(s) 140, 202, 359, 374, 571, 585
Sau3AI GATC 6 cut(s) 154, 613, 730, 802, 847, 1323
Sau96I GGNCC 5 cut(s) 242, 243, 256, 902, 1334
SchI GAGTC 7 cut(s) 20, 287, 530, 541, 709, 1140, 1223
ScrFI CCNGG 4 cut(s) 229, 241, 418, 419
SduI GDGCHC 5 cut(s) 246, 272, 564, 812, 1297
SfaNI GCATC 4 cut(s) 60, 127, 924, 1192
Sfr274I CTCGAG 1 cut(s) 929
Sfr303I CCGCGG 1 cut(s) 21
SfuI TTCGAA 1 cut(s) 1029
SgrBI CCGCGG 1 cut(s) 21
SinI GGWCC 3 cut(s) 256, 902, 1334
SlaI CTCGAG 1 cut(s) 929
SmaI CCCGGG 1 cut(s) 419
SmlI CTYRAG 4 cut(s) 473, 500, 929, 1070
SmoI CTYRAG 4 cut(s) 473, 500, 929, 1070
Sse9I AATT 7 cut(s) 675, 763, 984, 1056, 1169, 1307, 1390
SsiI CCGC 8 cut(s) 18, 20, 123, 140, 201, 358, 402, 1053
SspMI CTAG 5 cut(s) 111, 177, 567, 774, 1046
SstI GAGCTC 2 cut(s) 272, 1297
StyD4I CCNGG 4 cut(s) 227, 239, 416, 417
StyI CCWWGG 1 cut(s) 176
TaaI ACNGT 6 cut(s) 60, 742, 902, 923, 974, 1253
TaqI TCGA 5 cut(s) 24, 151, 930, 1029, 1297
TasI AATT 7 cut(s) 675, 763, 984, 1056, 1169, 1307, 1390
TatI WGTACW 2 cut(s) 969, 974
TauI GCSGC 3 cut(s) 142, 204, 361
TfiI GAWTC 7 cut(s) 181, 194, 262, 293, 301, 455, 866
TscAI CASTG 4 cut(s) 63, 93, 1204, 1229
TseFI GTSAC 2 cut(s) 45, 522
TseI GCWGC 3 cut(s) 373, 570, 584
Tsp45I GTSAC 2 cut(s) 45, 522
TspDTI ATGAA 4 cut(s) 469, 542, 924, 1287
TspGWI ACGGA 7 cut(s) 85, 795, 1099, 1103, 1236, 1248, 1359
TspMI CCCGGG 1 cut(s) 417
TspRI CASTG 4 cut(s) 63, 93, 1204, 1229
VneI GTGCAC 1 cut(s) 808
VpaK11BI GGWCC 3 cut(s) 256, 902, 1334
XagI CCTNNNNNAGG 1 cut(s) 1100
XapI RAATTY 2 cut(s) 1056, 1390
XbaI TCTAGA 1 cut(s) 1045
XhoI CTCGAG 1 cut(s) 929
XmaI CCCGGG 1 cut(s) 417
XmaJI CCTAGG 1 cut(s) 176
XmiI GTMKAC 2 cut(s) 24, 843
XmnI GAANNNNTTC 1 cut(s) 549
XspI CTAG 5 cut(s) 111, 177, 567, 774, 1046
Zsp2I ATGCAT 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.