pycom01g18170

rhodanese-like domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0012988
Physical Location & Seq
Forward (+)
17261986 .. 17264238
2253 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g18170.1

Sequence Viewer

Length: 1338 bp
ATGCCAAGGTGGAGGTCACCTCCATCACTGTCGCTGAGGATGCTATCGTCACCTCCACTGCACTCCGTGAGCCCTAACCCTAGATTCCACCACGCTTCTTCAATTTCGCCGCCCTCCAATCGACCTCCTCAGCCGTTCTTCAACCCCAGTAGGATTCCGGTCTCCGATTCTCGCGGCTTCCCTCTAAGCATGACGCTTTCCAGGTGCTTCTCCGGGCCCACCAGTCCGGTCCCGATTCCCAGCTCACCCGGGTCGGATAACTCGGATTCGCAGATTCTCGTGGTGGTCTCCTTCTACAAGTTCGCTGATTTTCCTGACCATGCTCGTCTGCGGCAACCATTGAAGCAGCTCTGCGAAGAACTGCTTTGCGGTATCATACTTGCACCGGAAGGGATAAACGGGAGCATTTGTGGGACAAGAGAATCGGTGGAGAAAGTTCTTGAGTTCATCCAGAGTGATAACCGCCTCAAGGGACTAAGAAGATTGGAGTCACCTGTGAGTCCTGAGGATGAAGCCATCCATCATGGACACACTAGTAGTTCTCCTCTGGCGGCAGGGGAAGACGCGCCTTTCAGATGGGATCATGTGAGGGTCAAGTTGAAGAAGGAGATTGTTAGTCTTGGAATGCCTGATGTATCGCCAATTGAAAAGGTCGGAAAGTATGTGAGTCCACGGGATTGGAATACATTAATTAGCGATCCAGATACGGTAGTTATTGATGTACGGAATAATTATGAAACTAGAATAGGAAAGTTCAAAGGAGCAGTTGATCCAAGTACTAAAGCATTCCGGGAGTTCCCATCTTGGGTAGACGATCAGTTTCAACCTTCCGAACCTGAGAATGGTGATCCGGAGGTGCAAGTCAAGAGTCCAAATGGAAGCCCCAACAATCAGCTGGATACATCAAAACCAAAAACATCTCTGAAAGTTGCCATGTACTGTACTGGGGGCATTAGATGTGAGAAAGCTTCAAGTTTTCTCCTCAGCAAAGGCTTCGAAGAGGTCTATCATCTAGAAGGTGGAATTCTGAAGTATCTTGAGGAAGTTCCGCCGACAGAGAGCCTGTGGGAGGGAGAGTGCTTCGTCTTTGACAAGAGGGTCTCAGTTGAGCATGGCTTAGCACAGGGAACTCACAAGTTATGCTATGGGTGTAAGCAACCGGTCAGTGATGCAGATATGGAGTCTCCTCAGTGGGAATACGGAGTTACTTGTCCGTACTGTTTCTCGTCAAAATCTGATGAAGAGAAGGAGAGGGCACGAGCTCGACAAAGGCAGTTTGAGACATGGGGGATCATCGGTGGTCCGGACAAGGGACGCAAACCGGATAGTATCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

446

Amino Acids

49.54

Weight (kDa)

6.3

Isoelectric Point (pI)

58.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0176_N PF17773 94 - 204 5.2e-18 UPF0176 acylphosphatase like domain
Rhodanese PF00581 228 - 347 1.9e-07 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40760
fragaria_vesca FvH4_7g26040
malus_domestica MD01G1168900.v1.1 MD07G1234800.v1.1
prunus_persica Prupe.2G265100_v2.0.a1
pyrus_communis pycom01g18170 pycom07g21240
rosa_chinensis RchiOBHm_Chr1g0372451
rosa_laevigata RLG00000026897
rosa_multiflora Rmu_sc0011709.1_g000010 Rmu_sc0019374.1_g000001
rosa_roxburghii Rroxscaffold_4G00284910
rosa_rugosa Rorug01G0372000
rosa_samantha Rh1AG380500 Rh1BG344100 Rh1CG357500 Rh1DG375300
rosa_wichuraiana Rw1G033460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1097
AccI GTMKAC 1 cut(s) 810
AccII CGCG 2 cut(s) 174, 566
AccIII TCCGGA 2 cut(s) 850, 1303
AciI CCGC 7 cut(s) 110, 174, 331, 369, 463, 551, 1049
AclWI GGATC 5 cut(s) 588, 692, 764, 842, 1298
AcsI RAATTY 1 cut(s) 1023
AcuI CTGAAG 1 cut(s) 1049
AdeI CACNNNGTG 1 cut(s) 67
AfaI GTAC 5 cut(s) 723, 778, 938, 943, 1217
AfiI CCNNNNNNNGG 5 cut(s) 469, 805, 842, 1069, 1310
AgeI ACCGGT 1 cut(s) 1159
AgsI TTSAA 8 cut(s) 102, 142, 343, 601, 647, 757, 824, 972
AhlI ACTAGT 1 cut(s) 533
AjnI CCWGG 1 cut(s) 200
AluBI AGCT 5 cut(s) 243, 349, 895, 968, 1262
AluI AGCT 5 cut(s) 243, 349, 895, 968, 1262
Alw21I GWGCWC 1 cut(s) 1264
Alw26I GTCTC 5 cut(s) 166, 292, 1105, 1188, 1274
AlwI GGATC 5 cut(s) 588, 692, 764, 842, 1298
Ama87I CYCGRG 1 cut(s) 248
Aor13HI TCCGGA 2 cut(s) 850, 1303
AoxI GGCC 1 cut(s) 215
ApaI GGGCCC 1 cut(s) 219
ApeKI GCWGC 1 cut(s) 346
ApoI RAATTY 1 cut(s) 1023
AseI ATTAAT 1 cut(s) 689
AsiGI ACCGGT 1 cut(s) 1159
AspLEI GCGC 1 cut(s) 568
AspS9I GGNCC 4 cut(s) 215, 216, 229, 1301
AsuC2I CCSGG 4 cut(s) 214, 249, 250, 791
AsuHPI GGTGA 5 cut(s) 9, 42, 237, 483, 857
AsuII TTCGAA 1 cut(s) 996
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 2 cut(s) 229, 1301
AxyI CCTNAGG 1 cut(s) 504
BaeGI GKGCMC 2 cut(s) 219, 1258
BaeI ACNNNNGTAYC 2 cut(s) 696, 729
BanII GRGCYC 3 cut(s) 74, 219, 1264
BauI CACGAG 2 cut(s) 278, 1257
BbsI GAAGAC 1 cut(s) 567
Bbv12I GWGCWC 1 cut(s) 1264
BbvCI CCTCAGC 3 cut(s) 35, 129, 983
BbvI GCAGC 1 cut(s) 358
BccI CCATC 5 cut(s) 31, 524, 528, 570, 808
BceAI ACGGC 1 cut(s) 118
BcgI CGANNNNNNTGC 2 cut(s) 976, 1010
BciT130I CCWGG 1 cut(s) 202
BciVI GTATCC 1 cut(s) 892
BcnI CCSGG 4 cut(s) 214, 249, 250, 791
BcoDI GTCTC 5 cut(s) 166, 292, 1105, 1188, 1274
BcuI ACTAGT 1 cut(s) 533
BfaI CTAG 4 cut(s) 81, 534, 741, 1013
BfuI GTATCC 1 cut(s) 892
BisI GCNGC 5 cut(s) 110, 175, 332, 347, 552
BlpI GCTNAGC 1 cut(s) 1117
BlsI GCNGC 5 cut(s) 111, 176, 333, 348, 553
BmcAI AGTACT 1 cut(s) 778
Bme1390I CCNGG 5 cut(s) 202, 214, 249, 250, 791
Bme18I GGWCC 2 cut(s) 229, 1301
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 4 cut(s) 215, 216, 229, 1301
BmiI GGNNCC 2 cut(s) 217, 231
BmrFI CCNGG 5 cut(s) 202, 214, 249, 250, 791
BmrI ACTGGG 2 cut(s) 141, 954
BmsI GCATC 2 cut(s) 30, 1159
BmuI ACTGGG 2 cut(s) 141, 954
BpiI GAAGAC 1 cut(s) 567
BplI GAGNNNNNCTC 1 cut(s) 36
Bpu10I CCTNAGC 3 cut(s) 35, 129, 983
Bpu1102I GCTNAGC 1 cut(s) 1117
Bpu14I TTCGAA 1 cut(s) 996
BpuEI CTTGAG 3 cut(s) 452, 461, 1058
BpuMI CCSGG 4 cut(s) 214, 249, 250, 791
BsaI GGTCTC 3 cut(s) 166, 292, 1105
BsaJI CCNNGG 3 cut(s) 5, 248, 671
BsaWI WCCGGW 7 cut(s) 157, 226, 385, 850, 1159, 1303, 1321
BsaXI ACNNNNNCTCC 4 cut(s) 394, 424, 1061, 1091
Bsc4I CCNNNNNNNGG 5 cut(s) 469, 805, 842, 1069, 1310
Bse118I RCCGGY 1 cut(s) 1159
Bse1I ACTGG 3 cut(s) 147, 222, 949
Bse21I CCTNAGG 1 cut(s) 504
BseAI TCCGGA 2 cut(s) 850, 1303
BseBI CCWGG 1 cut(s) 202
BseDI CCNNGG 3 cut(s) 5, 248, 671
BseGI GGATG 4 cut(s) 45, 447, 514, 516
BseLI CCNNNNNNNGG 5 cut(s) 469, 805, 842, 1069, 1310
BseMII CTCAG 7 cut(s) 26, 143, 495, 828, 997, 1116, 1202
BseNI ACTGG 3 cut(s) 147, 222, 949
BseRI GAGGAG 4 cut(s) 117, 534, 971, 1176
BseSI GKGCMC 2 cut(s) 219, 1258
BseXI GCAGC 1 cut(s) 358
BseYI CCCAGC 1 cut(s) 239
BsgI GTGCAG 1 cut(s) 44
Bsh1236I CGCG 2 cut(s) 174, 566
BshFI GGCC 1 cut(s) 217
BshTI ACCGGT 1 cut(s) 1159
BsiHKAI GWGCWC 1 cut(s) 1264
BsiHKCI CYCGRG 1 cut(s) 248
BslFI GGGAC 4 cut(s) 215, 427, 486, 1326
BslI CCNNNNNNNGG 5 cut(s) 469, 805, 842, 1069, 1310
BsmAI GTCTC 5 cut(s) 166, 292, 1105, 1188, 1274
BsmFI GGGAC 4 cut(s) 215, 427, 486, 1326
BsmI GAATGC 2 cut(s) 630, 785
BsnI GGCC 1 cut(s) 217
Bso31I GGTCTC 3 cut(s) 166, 292, 1105
BsoBI CYCGRG 1 cut(s) 248
Bsp119I TTCGAA 1 cut(s) 996
Bsp120I GGGCCC 1 cut(s) 215
Bsp1286I GDGCHC 4 cut(s) 74, 219, 1258, 1264
Bsp13I TCCGGA 2 cut(s) 850, 1303
Bsp143I GATC 6 cut(s) 580, 697, 769, 814, 847, 1290
Bsp1720I GCTNAGC 1 cut(s) 1117
BspACI CCGC 7 cut(s) 110, 174, 331, 369, 463, 551, 1049
BspANI GGCC 1 cut(s) 217
BspCNI CTCAG 7 cut(s) 27, 142, 496, 829, 996, 1115, 1201
BspEI TCCGGA 2 cut(s) 850, 1303
BspFNI CGCG 2 cut(s) 174, 566
BspLI GGNNCC 2 cut(s) 217, 231
BspPI GGATC 5 cut(s) 588, 692, 764, 842, 1298
BspT104I TTCGAA 1 cut(s) 996
BspTNI GGTCTC 3 cut(s) 166, 292, 1105
BsrFI RCCGGY 1 cut(s) 1159
BsrI ACTGG 3 cut(s) 147, 222, 949
BssAI RCCGGY 1 cut(s) 1159
BssECI CCNNGG 3 cut(s) 5, 248, 671
BssMI GATC 6 cut(s) 580, 697, 769, 814, 847, 1290
BssSI CACGAG 2 cut(s) 278, 1257
BssT1I CCWWGG 1 cut(s) 5
Bst2BI CACGAG 2 cut(s) 278, 1257
Bst2UI CCWGG 1 cut(s) 202
Bst4CI ACNGT 4 cut(s) 30, 709, 941, 1220
Bst6I CTCTTC 2 cut(s) 993, 1236
BstBI TTCGAA 1 cut(s) 996
BstDSI CCRYGG 1 cut(s) 671
BstEII GGTNACC 1 cut(s) 15
BstENI CCTNNNNNAGG 1 cut(s) 1067
BstF5I GGATG 4 cut(s) 45, 447, 514, 516
BstFNI CGCG 2 cut(s) 174, 566
BstHHI GCGC 1 cut(s) 568
BstKTI GATC 6 cut(s) 583, 700, 772, 817, 850, 1293
BstMAI GTCTC 5 cut(s) 166, 292, 1105, 1188, 1274
BstMBI GATC 6 cut(s) 580, 697, 769, 814, 847, 1290
BstMWI GCNNNNNNNGC 1 cut(s) 40
BstNI CCWGG 1 cut(s) 202
BstPI GGTNACC 1 cut(s) 15
BstSCI CCNGG 5 cut(s) 200, 212, 247, 248, 789
BstSLI GKGCMC 2 cut(s) 219, 1258
BstUI CGCG 2 cut(s) 174, 566
BstV1I GCAGC 1 cut(s) 358
BstV2I GAAGAC 1 cut(s) 567
BstXI CCANNNNNNTGG 1 cut(s) 678
Bsu36I CCTNAGG 1 cut(s) 504
BsuI GTATCC 1 cut(s) 892
BsuRI GGCC 1 cut(s) 217
BtgI CCRYGG 1 cut(s) 671
BtsCI GGATG 4 cut(s) 45, 447, 514, 516
BtsI GCAGTG 1 cut(s) 56
BtsIMutI CAGTG 4 cut(s) 26, 56, 1171, 1196
CfoI GCGC 1 cut(s) 568
Cfr10I RCCGGY 1 cut(s) 1159
Cfr13I GGNCC 4 cut(s) 215, 216, 229, 1301
Cfr9I CCCGGG 1 cut(s) 248
CseI GACGC 3 cut(s) 202, 572, 1323
Csp6I GTAC 5 cut(s) 722, 777, 937, 942, 1216
CspAI ACCGGT 1 cut(s) 1159
CviAII CATG 7 cut(s) 190, 320, 524, 584, 934, 1112, 1284
CviQI GTAC 5 cut(s) 722, 777, 937, 942, 1216
DpnI GATC 6 cut(s) 582, 699, 771, 816, 849, 1292
DpnII GATC 6 cut(s) 580, 697, 769, 814, 847, 1290
DraIII CACNNNGTG 1 cut(s) 67
DrdI GACNNNNNNGTC 1 cut(s) 1097
DseDI GACNNNNNNGTC 1 cut(s) 1097
Eam1104I CTCTTC 2 cut(s) 993, 1236
EarI CTCTTC 2 cut(s) 993, 1236
EciI GGCGGA 1 cut(s) 1038
Ecl136II GAGCTC 1 cut(s) 1262
Eco130I CCWWGG 1 cut(s) 5
Eco24I GRGCYC 3 cut(s) 74, 219, 1264
Eco31I GGTCTC 3 cut(s) 166, 292, 1105
Eco47I GGWCC 2 cut(s) 229, 1301
Eco53kI GAGCTC 1 cut(s) 1262
Eco57I CTGAAG 1 cut(s) 1049
Eco81I CCTNAGG 1 cut(s) 504
Eco88I CYCGRG 1 cut(s) 248
Eco91I GGTNACC 1 cut(s) 15
EcoICRI GAGCTC 1 cut(s) 1262
EcoNI CCTNNNNNAGG 1 cut(s) 1067
EcoO65I GGTNACC 1 cut(s) 15
EcoRI GAATTC 1 cut(s) 1023
EcoRII CCWGG 1 cut(s) 200
EcoT14I CCWWGG 1 cut(s) 5
EcoT38I GRGCYC 3 cut(s) 74, 219, 1264
ErhI CCWWGG 1 cut(s) 5
FaeI CATG 7 cut(s) 193, 323, 527, 587, 937, 1115, 1287
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FaqI GGGAC 4 cut(s) 215, 427, 486, 1326
FatI CATG 7 cut(s) 189, 319, 523, 583, 933, 1111, 1283
FblI GTMKAC 1 cut(s) 810
Fnu4HI GCNGC 5 cut(s) 110, 175, 332, 347, 552
FokI GGATG 4 cut(s) 52, 434, 503, 521
FriOI GRGCYC 3 cut(s) 74, 219, 1264
Fsp4HI GCNGC 5 cut(s) 110, 175, 332, 347, 552
FspBI CTAG 4 cut(s) 81, 534, 741, 1013
GlaI GCGC 1 cut(s) 567
GluI GCNGC 5 cut(s) 110, 175, 332, 347, 552
GsaI CCCAGC 1 cut(s) 243
HaeIII GGCC 1 cut(s) 217
HgaI GACGC 3 cut(s) 202, 572, 1323
HhaI GCGC 1 cut(s) 568
Hin1II CATG 7 cut(s) 193, 323, 527, 587, 937, 1115, 1287
Hin6I GCGC 1 cut(s) 566
HinP1I GCGC 1 cut(s) 566
HindIII AAGCTT 1 cut(s) 966
HphI GGTGA 5 cut(s) 9, 42, 237, 483, 857
Hpy166II GTNNAC 2 cut(s) 671, 811
Hpy188I TCNGA 9 cut(s) 166, 256, 265, 575, 656, 832, 924, 1029, 1237
Hpy8I GTNNAC 2 cut(s) 671, 811
HpyAV CCTTC 6 cut(s) 301, 383, 598, 837, 1010, 1240
HpyCH4III ACNGT 4 cut(s) 30, 709, 941, 1220
HpyCH4V TGCA 4 cut(s) 61, 383, 859, 1172
HpyF10VI GCNNNNNNNGC 1 cut(s) 40
Hsp92II CATG 7 cut(s) 193, 323, 527, 587, 937, 1115, 1287
HspAI GCGC 1 cut(s) 566
Kpn2I TCCGGA 2 cut(s) 850, 1303
Kzo9I GATC 6 cut(s) 580, 697, 769, 814, 847, 1290
LmnI GCTCC 2 cut(s) 402, 761
Lsp1109I GCAGC 1 cut(s) 358
LweI GCATC 2 cut(s) 30, 1159
MaeI CTAG 4 cut(s) 81, 534, 741, 1013
MaeIII GTNAC 4 cut(s) 15, 48, 489, 1204
MalI GATC 6 cut(s) 582, 699, 771, 816, 849, 1292
MboI GATC 6 cut(s) 580, 697, 769, 814, 847, 1290
MboII GAAGA 8 cut(s) 90, 130, 368, 492, 572, 613, 1010, 1253
MfeI CAATTG 1 cut(s) 642
MhlI GDGCHC 4 cut(s) 74, 219, 1258, 1264
MluCI AATT 5 cut(s) 102, 642, 690, 730, 1023
MlyI GAGTC 5 cut(s) 497, 508, 676, 877, 1190
MmeI TCCRAC 2 cut(s) 234, 634
MroI TCCGGA 2 cut(s) 850, 1303
MseI TTAA 1 cut(s) 689
MspA1I CMGCKG 1 cut(s) 895
MspR9I CCNGG 5 cut(s) 202, 214, 249, 250, 791
MunI CAATTG 1 cut(s) 642
Mva1269I GAATGC 2 cut(s) 630, 785
MvaI CCWGG 1 cut(s) 202
MvnI CGCG 2 cut(s) 174, 566
MwoI GCNNNNNNNGC 1 cut(s) 40
NciI CCSGG 4 cut(s) 214, 249, 250, 791
NdeII GATC 6 cut(s) 580, 697, 769, 814, 847, 1290
NlaIII CATG 7 cut(s) 193, 323, 527, 587, 937, 1115, 1287
NlaIV GGNNCC 2 cut(s) 217, 231
NmuCI GTSAC 3 cut(s) 15, 48, 489
NspV TTCGAA 1 cut(s) 996
PctI GAATGC 2 cut(s) 630, 785
PfeI GAWTC 7 cut(s) 84, 154, 167, 235, 266, 274, 422
PfoI TCCNGGA 1 cut(s) 789
PinAI ACCGGT 1 cut(s) 1159
PkrI GCNGC 5 cut(s) 111, 176, 333, 348, 553
PleI GAGTC 5 cut(s) 496, 507, 675, 876, 1189
PpsI GAGTC 5 cut(s) 496, 507, 675, 876, 1189
PshBI ATTAAT 1 cut(s) 689
Psp124BI GAGCTC 1 cut(s) 1264
Psp6I CCWGG 1 cut(s) 200
PspEI GGTNACC 1 cut(s) 15
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 1 cut(s) 200
PspN4I GGNNCC 2 cut(s) 217, 231
PspOMI GGGCCC 1 cut(s) 215
PspPI GGNCC 4 cut(s) 215, 216, 229, 1301
PvuII CAGCTG 1 cut(s) 895
RsaI GTAC 5 cut(s) 723, 778, 938, 943, 1217
RsaNI GTAC 5 cut(s) 722, 777, 937, 942, 1216
SacI GAGCTC 1 cut(s) 1264
SaqAI TTAA 1 cut(s) 689
SatI GCNGC 5 cut(s) 110, 175, 332, 347, 552
Sau3AI GATC 6 cut(s) 580, 697, 769, 814, 847, 1290
Sau96I GGNCC 4 cut(s) 215, 216, 229, 1301
ScaI AGTACT 1 cut(s) 778
SchI GAGTC 5 cut(s) 497, 508, 676, 877, 1190
ScrFI CCNGG 5 cut(s) 202, 214, 249, 250, 791
SduI GDGCHC 4 cut(s) 74, 219, 1258, 1264
SfaNI GCATC 2 cut(s) 30, 1159
SfuI TTCGAA 1 cut(s) 996
SinI GGWCC 2 cut(s) 229, 1301
SmaI CCCGGG 1 cut(s) 250
SmlI CTYRAG 3 cut(s) 440, 467, 1037
SmoI CTYRAG 3 cut(s) 440, 467, 1037
SpeI ACTAGT 1 cut(s) 533
Sse9I AATT 5 cut(s) 102, 642, 690, 730, 1023
SsiI CCGC 7 cut(s) 110, 174, 331, 369, 463, 551, 1049
SspMI CTAG 4 cut(s) 81, 534, 741, 1013
SstI GAGCTC 1 cut(s) 1264
StyD4I CCNGG 5 cut(s) 200, 212, 247, 248, 789
StyI CCWWGG 1 cut(s) 5
TaaI ACNGT 4 cut(s) 30, 709, 941, 1220
TaqI TCGA 3 cut(s) 121, 996, 1264
TasI AATT 5 cut(s) 102, 642, 690, 730, 1023
TatI WGTACW 3 cut(s) 776, 936, 941
TauI GCSGC 4 cut(s) 112, 177, 334, 554
TfiI GAWTC 7 cut(s) 84, 154, 167, 235, 266, 274, 422
Tru1I TTAA 1 cut(s) 689
Tru9I TTAA 1 cut(s) 689
TscAI CASTG 4 cut(s) 33, 63, 1171, 1196
TseFI GTSAC 3 cut(s) 15, 48, 489
TseI GCWGC 1 cut(s) 346
Tsp45I GTSAC 3 cut(s) 15, 48, 489
TspDTI ATGAA 4 cut(s) 436, 525, 750, 1254
TspGWI ACGGA 4 cut(s) 55, 739, 1203, 1215
TspMI CCCGGG 1 cut(s) 248
TspRI CASTG 4 cut(s) 33, 63, 1171, 1196
VpaK11BI GGWCC 2 cut(s) 229, 1301
VspI ATTAAT 1 cut(s) 689
XagI CCTNNNNNAGG 1 cut(s) 1067
XapI RAATTY 1 cut(s) 1023
XbaI TCTAGA 1 cut(s) 1012
XcmI CCANNNNNNNNNTGG 1 cut(s) 892
XmaI CCCGGG 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 810
XspI CTAG 4 cut(s) 81, 534, 741, 1013
ZrmI AGTACT 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.