Rroxscaffold_4G00284910

rhodanese-like domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012988
Physical Location & Seq
Reverse (-)
6419222 .. 6422081
2860 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00284910.1

Sequence Viewer

Length: 1350 bp
ATGCTATCTTCTTCTTCTTCATCCTCACTGCACTCCCTAAACCCTAACCCTAAATCACACCCCGCCTCTTCAATTCCCGATTCCTTCCCCAATCGCCGTCTTCAACTCTTCTCTAATCCAATTCCAAACCCAATTGGGATTTTTAAACACTGCAACCCTCAAAGTATGACTCTTTCTAGATGCTTGTCCGGGTCAACCAACCCAGTACCCATTTCGAGCTTACCCGAATCGGATGATCCGGATTCGAAAGTTCTGGTCGTGGTCTCCTTCTACAAGTTCGCTGATTTTCCTGACTGTGCTCGTTTGCGGCAACCCTTGAAGCAACTCTGCGAGGAATTGCGCGTTTCAGGTGGGATCATACTTGCACCGGAAGGGATCAATGGCAGCATATGCGGGAGCAGAGAGTCGGTGGAGAAAGTTCTGGAGTTCATTCAAAGTGATGAGCGCCTAAGGGGGCTGAGAAGATTGGAGTCACCTGTGAGTCCGGAGGATGAAGCCATTCATCACGGACACAGTAGCTGTTCTCCTCTGGCAGCAGGGGAGGATGCACCCTTCCGATGGGATCATGTGAGGGTCAAGTTGAAGAAGGAGATTGTTACTCTTGGCATGCCTGCTGTATCACCTACTGAAAAAGTTGGGAAGTATGTGAGTCCAAGTGAATGGAATGAACTGATTAGTGATCCAGATACGGTTGTAATTGATGTGCGCAATAACTATGAAACTAGAATTGGGAAGTTTAAGGGAGCAGTTGATCCGTGTACTAAAGCATTCCGGGAGTTCCCGTCTTGGGTAGAGGATCAATTCCAACATTCTGAACCGGAGAATGGGCATACAGAAACAAACGCCAACAGTTCAGGTGGAAGCACCAAAAATCAACCAGGGATTTCAAAACCAAAAACACCACCACGAGTTGCCATGTACTGTACTGGGGGCATTAGATGTGAGAAAGCTTCTAGTTATCTCCTTAGCAAAGGTTTTGAAGAGGTTTATCATCTAGAAGGTGGGATTCTGAAGTATCTTGAGGAAGTTCCAGAGAAAGAGAGCCTGTGGGAGGGAGAGTGCTTTGTCTTTGACAAGAGAGTCTCTGTTGAGCACGGTTTAGTACAAGGAAATCACAAGTTATGCTTTGGGTGTAAGCAACCAGTCAGTGATGCTGATATGGAGTCTCCTCAGTGGGAATACGGGGTTTCATGTCCCTATTGTTACTCGTCAAAGACCATCGAAGAAAAGGAAAGGGCAAGAGCTCGACAAAGGCAGTTTGAGACATGGGGTATCATTGGCGGTCCAGACAAGGGCCGTAAGCCAGATACTATTAAACAGAGGCCTACTCAACTGTCAAATTCAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

449

Amino Acids

49.86

Weight (kDa)

6.22

Isoelectric Point (pI)

52.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0176_N PF17773 86 - 198 1.9e-22 UPF0176 acylphosphatase like domain
Rhodanese PF00581 218 - 341 3.8e-07 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40760
fragaria_vesca FvH4_7g26040
malus_domestica MD01G1168900.v1.1 MD07G1234800.v1.1
prunus_persica Prupe.2G265100_v2.0.a1
pyrus_communis pycom01g18170 pycom07g21240
rosa_chinensis RchiOBHm_Chr1g0372451
rosa_laevigata RLG00000026897
rosa_multiflora Rmu_sc0011709.1_g000010 Rmu_sc0019374.1_g000001
rosa_roxburghii Rroxscaffold_4G00284910
rosa_rugosa Rorug01G0372000
rosa_samantha Rh1AG380500 Rh1BG344100 Rh1CG357500 Rh1DG375300
rosa_wichuraiana Rw1G033460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1079
Acc16I TGCGCA 1 cut(s) 707
AccII CGCG 1 cut(s) 342
AccIII TCCGGA 2 cut(s) 238, 484
AciI CCGC 4 cut(s) 63, 307, 393, 1281
AclWI GGATC 7 cut(s) 230, 362, 383, 570, 674, 746, 804
AcsI RAATTY 1 cut(s) 1339
AcuI CTGAAG 1 cut(s) 1031
AfaI GTAC 5 cut(s) 207, 760, 920, 925, 1104
AfiI CCNNNNNNNGG 5 cut(s) 558, 787, 824, 1051, 1292
AgsI TTSAA 8 cut(s) 72, 104, 319, 434, 583, 888, 980, 1344
AjnI CCWGG 1 cut(s) 877
AjuI GAANNNNNNNTTGG 2 cut(s) 711, 743
AluBI AGCT 4 cut(s) 219, 519, 950, 1244
AluI AGCT 4 cut(s) 219, 519, 950, 1244
Alw21I GWGCWC 3 cut(s) 301, 1095, 1246
Alw26I GTCTC 4 cut(s) 268, 1087, 1170, 1256
AlwI GGATC 7 cut(s) 230, 362, 383, 570, 674, 746, 804
AlwNI CAGNNNCTG 1 cut(s) 519
Aor13HI TCCGGA 2 cut(s) 238, 484
AoxI GGCC 2 cut(s) 1294, 1322
ApeKI GCWGC 2 cut(s) 384, 533
ApoI RAATTY 1 cut(s) 1339
Asp700I GAANNNNTTC 1 cut(s) 498
AspLEI GCGC 3 cut(s) 342, 447, 708
AspS9I GGNCC 2 cut(s) 1283, 1294
AsuC2I CCSGG 2 cut(s) 190, 773
AsuHPI GGTGA 2 cut(s) 465, 612
AsuII TTCGAA 1 cut(s) 245
AvaII GGWCC 1 cut(s) 1283
AxyI CCTNAGG 1 cut(s) 449
BaeI ACNNNNGTAYC 2 cut(s) 678, 711
BanII GRGCYC 1 cut(s) 1246
BauI CACGAG 1 cut(s) 906
BbsI GAAGAC 1 cut(s) 92
Bbv12I GWGCWC 3 cut(s) 301, 1095, 1246
BbvI GCAGC 2 cut(s) 396, 545
BccI CCATC 2 cut(s) 552, 1226
BceAI ACGGC 2 cut(s) 81, 1281
BciT130I CCWGG 1 cut(s) 879
BcnI CCSGG 2 cut(s) 190, 773
BcoDI GTCTC 4 cut(s) 268, 1087, 1170, 1256
BfaI CTAG 4 cut(s) 177, 723, 954, 995
BfoI RGCGCY 1 cut(s) 448
BisI GCNGC 3 cut(s) 308, 385, 534
BlsI GCNGC 3 cut(s) 309, 386, 535
Bme1390I CCNGG 3 cut(s) 190, 773, 879
Bme18I GGWCC 1 cut(s) 1283
BmgT120I GGNCC 2 cut(s) 1283, 1294
BmrFI CCNGG 3 cut(s) 190, 773, 879
BmrI ACTGGG 2 cut(s) 197, 936
BmsI GCATC 3 cut(s) 170, 535, 1141
BmuI ACTGGG 2 cut(s) 197, 936
BpiI GAAGAC 1 cut(s) 92
BplI GAGNNNNNCTC 2 cut(s) 1312, 1344
BpmI CTGGAG 1 cut(s) 443
Bpu10I CCTNAGC 1 cut(s) 965
Bpu14I TTCGAA 1 cut(s) 245
BpuEI CTTGAG 1 cut(s) 1040
BpuMI CCSGG 2 cut(s) 190, 773
BsaI GGTCTC 1 cut(s) 268
BsaJI CCNNGG 1 cut(s) 878
BsaWI WCCGGW 4 cut(s) 238, 367, 484, 817
BsaXI ACNNNNNCTCC 4 cut(s) 767, 797, 1043, 1073
Bsc4I CCNNNNNNNGG 5 cut(s) 558, 787, 824, 1051, 1292
Bse1I ACTGG 3 cut(s) 203, 931, 1142
Bse21I CCTNAGG 1 cut(s) 449
BseAI TCCGGA 2 cut(s) 238, 484
BseBI CCWGG 1 cut(s) 879
BseDI CCNNGG 1 cut(s) 878
BseGI GGATG 4 cut(s) 20, 238, 496, 550
BseLI CCNNNNNNNGG 5 cut(s) 558, 787, 824, 1051, 1292
BseMII CTCAG 2 cut(s) 449, 1184
BseNI ACTGG 3 cut(s) 203, 931, 1142
BseRI GAGGAG 2 cut(s) 516, 1158
BseXI GCAGC 2 cut(s) 396, 545
BsgI GTGCAG 1 cut(s) 14
Bsh1236I CGCG 1 cut(s) 342
BshFI GGCC 2 cut(s) 1296, 1324
BsiHKAI GWGCWC 3 cut(s) 301, 1095, 1246
BsiSI CCGG 6 cut(s) 189, 239, 368, 485, 772, 818
BslFI GGGAC 1 cut(s) 1179
BslI CCNNNNNNNGG 5 cut(s) 558, 787, 824, 1051, 1292
BsmAI GTCTC 4 cut(s) 268, 1087, 1170, 1256
BsmFI GGGAC 1 cut(s) 1179
BsmI GAATGC 1 cut(s) 767
BsnI GGCC 2 cut(s) 1296, 1324
Bso31I GGTCTC 1 cut(s) 268
Bsp119I TTCGAA 1 cut(s) 245
Bsp1286I GDGCHC 3 cut(s) 301, 1095, 1246
Bsp13I TCCGGA 2 cut(s) 238, 484
Bsp143I GATC 7 cut(s) 235, 354, 375, 562, 679, 751, 796
BspACI CCGC 4 cut(s) 63, 307, 393, 1281
BspANI GGCC 2 cut(s) 1296, 1324
BspCNI CTCAG 2 cut(s) 450, 1183
BspEI TCCGGA 2 cut(s) 238, 484
BspFNI CGCG 1 cut(s) 342
BspPI GGATC 7 cut(s) 230, 362, 383, 570, 674, 746, 804
BspT104I TTCGAA 1 cut(s) 245
BspTNI GGTCTC 1 cut(s) 268
BsrI ACTGG 3 cut(s) 203, 931, 1142
BssECI CCNNGG 1 cut(s) 878
BssMI GATC 7 cut(s) 235, 354, 375, 562, 679, 751, 796
BssSI CACGAG 1 cut(s) 906
Bst2BI CACGAG 1 cut(s) 906
Bst2UI CCWGG 1 cut(s) 879
Bst4CI ACNGT 7 cut(s) 296, 515, 691, 851, 923, 1097, 1335
Bst6I CTCTTC 3 cut(s) 73, 113, 975
BstAPI GCANNNNNTGC 1 cut(s) 390
BstBI TTCGAA 1 cut(s) 245
BstC8I GCNNGC 2 cut(s) 608, 612
BstDEI CTNAG 4 cut(s) 449, 458, 965, 1170
BstENI CCTNNNNNAGG 1 cut(s) 1049
BstF5I GGATG 4 cut(s) 20, 238, 496, 550
BstFNI CGCG 1 cut(s) 342
BstH2I RGCGCY 1 cut(s) 448
BstHHI GCGC 3 cut(s) 342, 447, 708
BstKTI GATC 7 cut(s) 238, 357, 378, 565, 682, 754, 799
BstMAI GTCTC 4 cut(s) 268, 1087, 1170, 1256
BstMBI GATC 7 cut(s) 235, 354, 375, 562, 679, 751, 796
BstMWI GCNNNNNNNGC 1 cut(s) 390
BstNI CCWGG 1 cut(s) 879
BstNSI RCATGY 1 cut(s) 610
BstSCI CCNGG 3 cut(s) 188, 771, 877
BstUI CGCG 1 cut(s) 342
BstV1I GCAGC 2 cut(s) 396, 545
BstV2I GAAGAC 1 cut(s) 92
BstXI CCANNNNNNTGG 1 cut(s) 660
Bsu36I CCTNAGG 1 cut(s) 449
BsuRI GGCC 2 cut(s) 1296, 1324
BtsCI GGATG 4 cut(s) 20, 238, 496, 550
BtsI GCAGTG 2 cut(s) 26, 148
BtsIMutI CAGTG 4 cut(s) 26, 148, 1153, 1178
Cac8I GCNNGC 2 cut(s) 608, 612
CaiI CAGNNNCTG 1 cut(s) 519
CfoI GCGC 3 cut(s) 342, 447, 708
Cfr13I GGNCC 2 cut(s) 1283, 1294
Csp6I GTAC 5 cut(s) 206, 759, 919, 924, 1103
CviAII CATG 5 cut(s) 566, 607, 916, 1191, 1266
CviQI GTAC 5 cut(s) 206, 759, 919, 924, 1103
DdeI CTNAG 4 cut(s) 449, 458, 965, 1170
DpnI GATC 7 cut(s) 237, 356, 377, 564, 681, 753, 798
DpnII GATC 7 cut(s) 235, 354, 375, 562, 679, 751, 796
DraI TTTAAA 1 cut(s) 145
DrdI GACNNNNNNGTC 1 cut(s) 1079
DseDI GACNNNNNNGTC 1 cut(s) 1079
Eam1104I CTCTTC 3 cut(s) 73, 113, 975
EarI CTCTTC 3 cut(s) 73, 113, 975
Ecl136II GAGCTC 1 cut(s) 1244
Eco147I AGGCCT 1 cut(s) 1324
Eco24I GRGCYC 1 cut(s) 1246
Eco31I GGTCTC 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 1283
Eco53kI GAGCTC 1 cut(s) 1244
Eco57I CTGAAG 1 cut(s) 1031
Eco81I CCTNAGG 1 cut(s) 449
EcoICRI GAGCTC 1 cut(s) 1244
EcoNI CCTNNNNNAGG 1 cut(s) 1049
EcoRII CCWGG 1 cut(s) 877
EcoT38I GRGCYC 1 cut(s) 1246
FaeI CATG 5 cut(s) 569, 610, 919, 1194, 1269
FalI AAGNNNNNCTT 2 cut(s) 1109, 1141
FaqI GGGAC 1 cut(s) 1179
FatI CATG 5 cut(s) 565, 606, 915, 1190, 1265
FauI CCCGC 2 cut(s) 70, 386
FauNDI CATATG 1 cut(s) 389
Fnu4HI GCNGC 3 cut(s) 308, 385, 534
FokI GGATG 4 cut(s) 7, 245, 503, 557
FriOI GRGCYC 1 cut(s) 1246
Fsp4HI GCNGC 3 cut(s) 308, 385, 534
FspBI CTAG 4 cut(s) 177, 723, 954, 995
FspI TGCGCA 1 cut(s) 707
GlaI GCGC 3 cut(s) 341, 446, 707
GluI GCNGC 3 cut(s) 308, 385, 534
GsuI CTGGAG 1 cut(s) 443
HaeII RGCGCY 1 cut(s) 448
HaeIII GGCC 2 cut(s) 1296, 1324
HapII CCGG 6 cut(s) 189, 239, 368, 485, 772, 818
HhaI GCGC 3 cut(s) 342, 447, 708
Hin1II CATG 5 cut(s) 569, 610, 919, 1194, 1269
Hin6I GCGC 3 cut(s) 340, 445, 706
HinP1I GCGC 3 cut(s) 340, 445, 706
HincII GTYRAC 1 cut(s) 195
HindII GTYRAC 1 cut(s) 195
HindIII AAGCTT 1 cut(s) 948
HpaII CCGG 6 cut(s) 189, 239, 368, 485, 772, 818
HphI GGTGA 2 cut(s) 465, 612
Hpy166II GTNNAC 2 cut(s) 195, 759
Hpy188I TCNGA 4 cut(s) 232, 557, 814, 1011
Hpy8I GTNNAC 2 cut(s) 195, 759
HpyAV CCTTC 6 cut(s) 94, 277, 365, 562, 580, 992
HpyCH4III ACNGT 7 cut(s) 296, 515, 691, 851, 923, 1097, 1335
HpyCH4V TGCA 4 cut(s) 31, 153, 365, 548
HpyF10VI GCNNNNNNNGC 1 cut(s) 390
HpyF3I CTNAG 4 cut(s) 449, 458, 965, 1170
Hsp92II CATG 5 cut(s) 569, 610, 919, 1194, 1269
HspAI GCGC 3 cut(s) 340, 445, 706
Kpn2I TCCGGA 2 cut(s) 238, 484
Kzo9I GATC 7 cut(s) 235, 354, 375, 562, 679, 751, 796
LmnI GCTCC 2 cut(s) 396, 743
Lsp1109I GCAGC 2 cut(s) 396, 545
LweI GCATC 3 cut(s) 170, 535, 1141
MaeI CTAG 4 cut(s) 177, 723, 954, 995
MaeIII GTNAC 3 cut(s) 471, 595, 1202
MalI GATC 7 cut(s) 237, 356, 377, 564, 681, 753, 798
MboI GATC 7 cut(s) 235, 354, 375, 562, 679, 751, 796
MfeI CAATTG 1 cut(s) 132
MhlI GDGCHC 3 cut(s) 301, 1095, 1246
MluCI AATT 9 cut(s) 72, 120, 132, 335, 696, 726, 800, 1339, 1344
MlyI GAGTC 7 cut(s) 163, 413, 479, 490, 658, 1089, 1172
MmeI TCCRAC 1 cut(s) 829
MroI TCCGGA 2 cut(s) 238, 484
MroXI GAANNNNTTC 1 cut(s) 498
MseI TTAA 3 cut(s) 144, 738, 1314
MspI CCGG 6 cut(s) 189, 239, 368, 485, 772, 818
MspR9I CCNGG 3 cut(s) 190, 773, 879
MunI CAATTG 1 cut(s) 132
Mva1269I GAATGC 1 cut(s) 767
MvaI CCWGG 1 cut(s) 879
MvnI CGCG 1 cut(s) 342
MwoI GCNNNNNNNGC 1 cut(s) 390
NciI CCSGG 2 cut(s) 190, 773
NdeI CATATG 1 cut(s) 389
NdeII GATC 7 cut(s) 235, 354, 375, 562, 679, 751, 796
NlaIII CATG 5 cut(s) 569, 610, 919, 1194, 1269
NmuCI GTSAC 1 cut(s) 471
NsbI TGCGCA 1 cut(s) 707
NspI RCATGY 1 cut(s) 610
NspV TTCGAA 1 cut(s) 245
PaeI GCATGC 1 cut(s) 610
PceI AGGCCT 1 cut(s) 1324
PctI GAATGC 1 cut(s) 767
PdmI GAANNNNTTC 1 cut(s) 498
PfeI GAWTC 4 cut(s) 80, 227, 242, 1006
PfoI TCCNGGA 1 cut(s) 771
PkrI GCNGC 3 cut(s) 309, 386, 535
PleI GAGTC 7 cut(s) 163, 412, 478, 489, 657, 1088, 1171
PpsI GAGTC 7 cut(s) 163, 412, 478, 489, 657, 1088, 1171
Psp124BI GAGCTC 1 cut(s) 1246
Psp6I CCWGG 1 cut(s) 877
PspGI CCWGG 1 cut(s) 877
PspPI GGNCC 2 cut(s) 1283, 1294
PstNI CAGNNNCTG 1 cut(s) 519
RsaI GTAC 5 cut(s) 207, 760, 920, 925, 1104
RsaNI GTAC 5 cut(s) 206, 759, 919, 924, 1103
SacI GAGCTC 1 cut(s) 1246
SaqAI TTAA 3 cut(s) 144, 738, 1314
SatI GCNGC 3 cut(s) 308, 385, 534
Sau3AI GATC 7 cut(s) 235, 354, 375, 562, 679, 751, 796
Sau96I GGNCC 2 cut(s) 1283, 1294
SchI GAGTC 7 cut(s) 163, 413, 479, 490, 658, 1089, 1172
ScrFI CCNGG 3 cut(s) 190, 773, 879
SduI GDGCHC 3 cut(s) 301, 1095, 1246
SfaNI GCATC 3 cut(s) 170, 535, 1141
SfuI TTCGAA 1 cut(s) 245
SinI GGWCC 1 cut(s) 1283
SmlI CTYRAG 1 cut(s) 1019
SmoI CTYRAG 1 cut(s) 1019
SphI GCATGC 1 cut(s) 610
Sse9I AATT 9 cut(s) 72, 120, 132, 335, 696, 726, 800, 1339, 1344
SseBI AGGCCT 1 cut(s) 1324
SsiI CCGC 4 cut(s) 63, 307, 393, 1281
SspMI CTAG 4 cut(s) 177, 723, 954, 995
SstI GAGCTC 1 cut(s) 1246
StuI AGGCCT 1 cut(s) 1324
StyD4I CCNGG 3 cut(s) 188, 771, 877
TaaI ACNGT 7 cut(s) 296, 515, 691, 851, 923, 1097, 1335
TaqI TCGA 4 cut(s) 215, 245, 1221, 1246
TasI AATT 9 cut(s) 72, 120, 132, 335, 696, 726, 800, 1339, 1344
TatI WGTACW 4 cut(s) 758, 918, 923, 1102
TauI GCSGC 1 cut(s) 310
TfiI GAWTC 4 cut(s) 80, 227, 242, 1006
Tru1I TTAA 3 cut(s) 144, 738, 1314
Tru9I TTAA 3 cut(s) 144, 738, 1314
TscAI CASTG 4 cut(s) 33, 155, 1153, 1178
TseFI GTSAC 1 cut(s) 471
TseI GCWGC 2 cut(s) 384, 533
Tsp45I GTSAC 1 cut(s) 471
TspDTI ATGAA 7 cut(s) 9, 418, 491, 507, 681, 732, 1179
TspGWI ACGGA 2 cut(s) 522, 744
TspRI CASTG 4 cut(s) 33, 155, 1153, 1178
VpaK11BI GGWCC 1 cut(s) 1283
XagI CCTNNNNNAGG 1 cut(s) 1049
XapI RAATTY 1 cut(s) 1339
XbaI TCTAGA 2 cut(s) 176, 994
XceI RCATGY 1 cut(s) 610
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 4 cut(s) 177, 723, 954, 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.