MD01G1168900.v1.1

rhodanese-like domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012988
Physical Location & Seq
Forward (+)
27299512 .. 27302139
2628 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1168900.v1.1.491

Sequence Viewer

Length: 1374 bp
ATGCTAAGGTGGAGGTCACCTCCATCACTGTCGCTGAGGATGCTATCGTCACCTCCACTGCACTCCGTGAGTCCTAACCCTAGATTCCACCACGCTTCTTCAATTTCGCCGCCCTCCAATCGACCTCCTCAGCCGTTCTTCAACCCCAGTAGGATTCCGGTCTCCGATTCTCGCGGCGTCCCTCAAAGCATGACGCTTTCCAGGTGCTTCTCCGGGCCCACCAGTCCGGTCCCGATTCCGAGCTCACCCGACTCGGATAACTCGGATTCGCAGATTCTCGTGGTGGTCTCCTTCTACAAGTTCGCTGATTTTCCTGACCATGCGCGCCTGCGGCAACCATTGAAGCAGCTCTGCGAAGAACTGCGTGTTTCAGGCGGTATCATACTTGCACCGGAAGGGATAAACGGGAGCATTTGTGGGACAAGAGAATCGGTGGAGAAAGTTCTTGAGTTCATCCAGAGTGATAACCGCCTTGAGGGACTAAGAAGATTGGAGTCACCTGTGAGTCCAGAGGATGAAGCCATCCATCATGGACACACTAGTAGTTCTCCTCTGGCAGCAGGGGAAGACGCGCCCTTCAGATGGGATCATGTGAGGGTCAAGTTGAAGAAGGAGATTGTTAGTCTTGGAATGCCTGATGTATCGCCAATTGAAAAGGTCGGAAAGTATGTGAGTCCACGGGATTGGAATACATTAATTAGCGATCCAGATACGGTAGTTATTGATGTGCGGAATAATTATGAAACTAGAATAGGGAAGTTCAAAGGAGCAGTTGATCCAAGTACTAAAGCATTCCGGGAGTTCCCATCTTGGGTAGACGATCAGTTTCAACCTTCTGAACCTGAGAATGGTGATCCTGAGGTGCAAGTCAAGAGTCCAAATGGAATCCCCAACAATCAGCTGGATACATCAAAACCAAAAACACCTCCGAAGGTTGCCATGTACTGTACTGGGGGCATTAGATGTGAGAAAGCTTCAAGTTTCCTCCTCAGCAAAGGCTTCGAAGAGGTCTATCATCTAGAAGGTGGAATTCTGAAGTATCTTGAGGAAGTTCCGCCGACAGAGAGCCTGTGGGAGGGAGAGTGCTTCGTCTTTGACAAGAGGGTCTCAGTTGAGCATGGCTTAGCACAGGGAACTCACAAGTTATGCTATGGGTGTAAGCAACCAGTCAGCGATGCAGATATGGAGTCTCCTCAGTGGGAATACGGAGTTACTTGTCCGTACTGTTTCTCGTCAAAATCTGATGAAGAGAAGGAGAGGGCACGAGCTCGACAAAGGCAGTTTGAGACATGGGGGATCATTGGTGGTCCGGACAAGGGACGCAAACCGGATAGTATCAAGCAGAACGCTACTCAACTTTCAAATTCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

458

Amino Acids

50.82

Weight (kDa)

6.05

Isoelectric Point (pI)

59.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0176_N PF17773 94 - 206 4.7e-22 UPF0176 acylphosphatase like domain
Rhodanese PF00581 230 - 349 6.8e-07 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40760
fragaria_vesca FvH4_7g26040
malus_domestica MD01G1168900.v1.1 MD07G1234800.v1.1
prunus_persica Prupe.2G265100_v2.0.a1
pyrus_communis pycom01g18170 pycom07g21240
rosa_chinensis RchiOBHm_Chr1g0372451
rosa_laevigata RLG00000026897
rosa_multiflora Rmu_sc0011709.1_g000010 Rmu_sc0019374.1_g000001
rosa_roxburghii Rroxscaffold_4G00284910
rosa_rugosa Rorug01G0372000
rosa_samantha Rh1AG380500 Rh1BG344100 Rh1CG357500 Rh1DG375300
rosa_wichuraiana Rw1G033460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1103
AccI GTMKAC 1 cut(s) 816
AccII CGCG 3 cut(s) 174, 325, 572
AccIII TCCGGA 1 cut(s) 1309
AciI CCGC 7 cut(s) 110, 174, 331, 375, 469, 730, 1055
AclWI GGATC 5 cut(s) 594, 698, 770, 848, 1304
AcsI RAATTY 2 cut(s) 1029, 1363
AcuI CTGAAG 2 cut(s) 562, 1055
AcyI GRCGYC 1 cut(s) 177
AdeI CACNNNGTG 1 cut(s) 67
AfaI GTAC 4 cut(s) 784, 944, 949, 1223
AfiI CCNNNNNNNGG 6 cut(s) 475, 582, 811, 848, 1075, 1316
AgsI TTSAA 9 cut(s) 102, 142, 343, 607, 653, 763, 830, 978, 1362
AhlI ACTAGT 1 cut(s) 539
AjnI CCWGG 1 cut(s) 200
AluBI AGCT 5 cut(s) 243, 349, 901, 974, 1268
AluI AGCT 5 cut(s) 243, 349, 901, 974, 1268
Alw21I GWGCWC 2 cut(s) 245, 1270
Alw26I GTCTC 5 cut(s) 166, 292, 1111, 1194, 1280
AlwI GGATC 5 cut(s) 594, 698, 770, 848, 1304
Aor13HI TCCGGA 1 cut(s) 1309
AoxI GGCC 1 cut(s) 215
ApaI GGGCCC 1 cut(s) 219
ApeKI GCWGC 2 cut(s) 346, 557
ApoI RAATTY 2 cut(s) 1029, 1363
AseI ATTAAT 1 cut(s) 695
AspLEI GCGC 3 cut(s) 325, 327, 574
AspS9I GGNCC 4 cut(s) 215, 216, 229, 1307
AsuC2I CCSGG 2 cut(s) 214, 797
AsuHPI GGTGA 5 cut(s) 9, 42, 237, 489, 863
AsuII TTCGAA 1 cut(s) 1002
AvaII GGWCC 2 cut(s) 229, 1307
AxyI CCTNAGG 1 cut(s) 858
BaeGI GKGCMC 2 cut(s) 219, 1264
BaeI ACNNNNGTAYC 2 cut(s) 702, 735
BanII GRGCYC 3 cut(s) 219, 245, 1270
BauI CACGAG 2 cut(s) 278, 1263
BbsI GAAGAC 1 cut(s) 573
Bbv12I GWGCWC 2 cut(s) 245, 1270
BbvCI CCTCAGC 3 cut(s) 35, 129, 989
BbvI GCAGC 2 cut(s) 358, 569
BccI CCATC 5 cut(s) 31, 530, 534, 576, 814
BceAI ACGGC 1 cut(s) 118
BcgI CGANNNNNNTGC 2 cut(s) 982, 1016
BciT130I CCWGG 1 cut(s) 202
BciVI GTATCC 1 cut(s) 898
BcnI CCSGG 2 cut(s) 214, 797
BcoDI GTCTC 5 cut(s) 166, 292, 1111, 1194, 1280
BcuI ACTAGT 1 cut(s) 539
BfaI CTAG 4 cut(s) 81, 540, 747, 1019
BfuI GTATCC 1 cut(s) 898
BisI GCNGC 5 cut(s) 110, 175, 332, 347, 558
BlpI GCTNAGC 1 cut(s) 1123
BlsI GCNGC 5 cut(s) 111, 176, 333, 348, 559
BmcAI AGTACT 1 cut(s) 784
Bme1390I CCNGG 3 cut(s) 202, 214, 797
Bme18I GGWCC 2 cut(s) 229, 1307
BmgT120I GGNCC 4 cut(s) 215, 216, 229, 1307
BmiI GGNNCC 2 cut(s) 217, 231
BmrFI CCNGG 3 cut(s) 202, 214, 797
BmrI ACTGGG 2 cut(s) 141, 960
BmsI GCATC 2 cut(s) 30, 1165
BmuI ACTGGG 2 cut(s) 141, 960
BpiI GAAGAC 1 cut(s) 573
BplI GAGNNNNNCTC 1 cut(s) 36
Bpu10I CCTNAGC 4 cut(s) 5, 35, 129, 989
Bpu1102I GCTNAGC 1 cut(s) 1123
Bpu14I TTCGAA 1 cut(s) 1002
BpuEI CTTGAG 3 cut(s) 467, 494, 1064
BpuMI CCSGG 2 cut(s) 214, 797
BsaHI GRCGYC 1 cut(s) 177
BsaI GGTCTC 3 cut(s) 166, 292, 1111
BsaJI CCNNGG 1 cut(s) 677
BsaWI WCCGGW 5 cut(s) 157, 226, 391, 1309, 1327
BsaXI ACNNNNNCTCC 4 cut(s) 400, 430, 1067, 1097
Bsc4I CCNNNNNNNGG 6 cut(s) 475, 582, 811, 848, 1075, 1316
Bse1I ACTGG 4 cut(s) 147, 222, 955, 1166
Bse21I CCTNAGG 1 cut(s) 858
BseAI TCCGGA 1 cut(s) 1309
BseBI CCWGG 1 cut(s) 202
BseDI CCNNGG 1 cut(s) 677
BseGI GGATG 4 cut(s) 45, 453, 520, 522
BseLI CCNNNNNNNGG 6 cut(s) 475, 582, 811, 848, 1075, 1316
BseMII CTCAG 7 cut(s) 26, 143, 834, 849, 1003, 1122, 1208
BseNI ACTGG 4 cut(s) 147, 222, 955, 1166
BsePI GCGCGC 1 cut(s) 323
BseRI GAGGAG 4 cut(s) 117, 540, 977, 1182
BseSI GKGCMC 2 cut(s) 219, 1264
BseXI GCAGC 2 cut(s) 358, 569
BsgI GTGCAG 1 cut(s) 44
Bsh1236I CGCG 3 cut(s) 174, 325, 572
BshFI GGCC 1 cut(s) 217
BsiHKAI GWGCWC 2 cut(s) 245, 1270
BsiSI CCGG 7 cut(s) 158, 213, 227, 392, 796, 1310, 1328
BslFI GGGAC 5 cut(s) 164, 215, 433, 492, 1332
BslI CCNNNNNNNGG 6 cut(s) 475, 582, 811, 848, 1075, 1316
BsmAI GTCTC 5 cut(s) 166, 292, 1111, 1194, 1280
BsmFI GGGAC 5 cut(s) 164, 215, 433, 492, 1332
BsmI GAATGC 2 cut(s) 636, 791
BsnI GGCC 1 cut(s) 217
Bso31I GGTCTC 3 cut(s) 166, 292, 1111
Bsp119I TTCGAA 1 cut(s) 1002
Bsp120I GGGCCC 1 cut(s) 215
Bsp1286I GDGCHC 4 cut(s) 219, 245, 1264, 1270
Bsp13I TCCGGA 1 cut(s) 1309
Bsp143I GATC 6 cut(s) 586, 703, 775, 820, 853, 1296
Bsp1720I GCTNAGC 1 cut(s) 1123
BspACI CCGC 7 cut(s) 110, 174, 331, 375, 469, 730, 1055
BspANI GGCC 1 cut(s) 217
BspCNI CTCAG 7 cut(s) 27, 142, 835, 850, 1002, 1121, 1207
BspEI TCCGGA 1 cut(s) 1309
BspFNI CGCG 3 cut(s) 174, 325, 572
BspLI GGNNCC 2 cut(s) 217, 231
BspPI GGATC 5 cut(s) 594, 698, 770, 848, 1304
BspT104I TTCGAA 1 cut(s) 1002
BspTNI GGTCTC 3 cut(s) 166, 292, 1111
BsrI ACTGG 4 cut(s) 147, 222, 955, 1166
BssECI CCNNGG 1 cut(s) 677
BssHII GCGCGC 1 cut(s) 323
BssMI GATC 6 cut(s) 586, 703, 775, 820, 853, 1296
BssNI GRCGYC 1 cut(s) 177
BssSI CACGAG 2 cut(s) 278, 1263
Bst2BI CACGAG 2 cut(s) 278, 1263
Bst2UI CCWGG 1 cut(s) 202
Bst4CI ACNGT 4 cut(s) 30, 715, 947, 1226
Bst6I CTCTTC 2 cut(s) 999, 1242
BstACI GRCGYC 1 cut(s) 177
BstBI TTCGAA 1 cut(s) 1002
BstC8I GCNNGC 2 cut(s) 325, 329
BstDSI CCRYGG 1 cut(s) 677
BstEII GGTNACC 1 cut(s) 15
BstENI CCTNNNNNAGG 1 cut(s) 1073
BstF5I GGATG 4 cut(s) 45, 453, 520, 522
BstFNI CGCG 3 cut(s) 174, 325, 572
BstHHI GCGC 3 cut(s) 325, 327, 574
BstKTI GATC 6 cut(s) 589, 706, 778, 823, 856, 1299
BstMAI GTCTC 5 cut(s) 166, 292, 1111, 1194, 1280
BstMBI GATC 6 cut(s) 586, 703, 775, 820, 853, 1296
BstMWI GCNNNNNNNGC 2 cut(s) 40, 331
BstNI CCWGG 1 cut(s) 202
BstPI GGTNACC 1 cut(s) 15
BstSCI CCNGG 3 cut(s) 200, 212, 795
BstSLI GKGCMC 2 cut(s) 219, 1264
BstUI CGCG 3 cut(s) 174, 325, 572
BstV1I GCAGC 2 cut(s) 358, 569
BstV2I GAAGAC 1 cut(s) 573
BstXI CCANNNNNNTGG 1 cut(s) 684
Bsu36I CCTNAGG 1 cut(s) 858
BsuI GTATCC 1 cut(s) 898
BsuRI GGCC 1 cut(s) 217
BtgI CCRYGG 1 cut(s) 677
BtgZI GCGATG 1 cut(s) 1188
BtsCI GGATG 4 cut(s) 45, 453, 520, 522
BtsI GCAGTG 1 cut(s) 56
BtsIMutI CAGTG 3 cut(s) 26, 56, 1202
Cac8I GCNNGC 2 cut(s) 325, 329
CfoI GCGC 3 cut(s) 325, 327, 574
Cfr13I GGNCC 4 cut(s) 215, 216, 229, 1307
CseI GACGC 4 cut(s) 166, 202, 578, 1329
Csp6I GTAC 4 cut(s) 783, 943, 948, 1222
CviAII CATG 7 cut(s) 190, 320, 530, 590, 940, 1118, 1290
CviQI GTAC 4 cut(s) 783, 943, 948, 1222
DpnI GATC 6 cut(s) 588, 705, 777, 822, 855, 1298
DpnII GATC 6 cut(s) 586, 703, 775, 820, 853, 1296
DraIII CACNNNGTG 1 cut(s) 67
DrdI GACNNNNNNGTC 1 cut(s) 1103
DseDI GACNNNNNNGTC 1 cut(s) 1103
Eam1104I CTCTTC 2 cut(s) 999, 1242
EarI CTCTTC 2 cut(s) 999, 1242
EciI GGCGGA 1 cut(s) 1044
Ecl136II GAGCTC 2 cut(s) 243, 1268
Eco24I GRGCYC 3 cut(s) 219, 245, 1270
Eco31I GGTCTC 3 cut(s) 166, 292, 1111
Eco47I GGWCC 2 cut(s) 229, 1307
Eco53kI GAGCTC 2 cut(s) 243, 1268
Eco57I CTGAAG 2 cut(s) 562, 1055
Eco81I CCTNAGG 1 cut(s) 858
Eco91I GGTNACC 1 cut(s) 15
EcoICRI GAGCTC 2 cut(s) 243, 1268
EcoNI CCTNNNNNAGG 1 cut(s) 1073
EcoO65I GGTNACC 1 cut(s) 15
EcoRI GAATTC 1 cut(s) 1029
EcoRII CCWGG 1 cut(s) 200
EcoT38I GRGCYC 3 cut(s) 219, 245, 1270
FaeI CATG 7 cut(s) 193, 323, 533, 593, 943, 1121, 1293
FaqI GGGAC 5 cut(s) 164, 215, 433, 492, 1332
FatI CATG 7 cut(s) 189, 319, 529, 589, 939, 1117, 1289
FblI GTMKAC 1 cut(s) 816
Fnu4HI GCNGC 5 cut(s) 110, 175, 332, 347, 558
FokI GGATG 4 cut(s) 52, 440, 509, 527
FriOI GRGCYC 3 cut(s) 219, 245, 1270
Fsp4HI GCNGC 5 cut(s) 110, 175, 332, 347, 558
FspBI CTAG 4 cut(s) 81, 540, 747, 1019
GlaI GCGC 3 cut(s) 324, 326, 573
GluI GCNGC 5 cut(s) 110, 175, 332, 347, 558
HaeIII GGCC 1 cut(s) 217
HapII CCGG 7 cut(s) 158, 213, 227, 392, 796, 1310, 1328
HgaI GACGC 4 cut(s) 166, 202, 578, 1329
HhaI GCGC 3 cut(s) 325, 327, 574
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 7 cut(s) 193, 323, 533, 593, 943, 1121, 1293
Hin6I GCGC 3 cut(s) 323, 325, 572
HinP1I GCGC 3 cut(s) 323, 325, 572
HindIII AAGCTT 1 cut(s) 972
HpaII CCGG 7 cut(s) 158, 213, 227, 392, 796, 1310, 1328
HphI GGTGA 5 cut(s) 9, 42, 237, 489, 863
Hpy166II GTNNAC 2 cut(s) 677, 817
Hpy8I GTNNAC 2 cut(s) 677, 817
HpyAV CCTTC 8 cut(s) 301, 389, 586, 604, 843, 925, 1016, 1246
HpyCH4III ACNGT 4 cut(s) 30, 715, 947, 1226
HpyCH4V TGCA 4 cut(s) 61, 389, 865, 1178
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 331
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 7 cut(s) 193, 323, 533, 593, 943, 1121, 1293
HspAI GCGC 3 cut(s) 323, 325, 572
Kpn2I TCCGGA 1 cut(s) 1309
Kzo9I GATC 6 cut(s) 586, 703, 775, 820, 853, 1296
LmnI GCTCC 2 cut(s) 408, 767
Lsp1109I GCAGC 2 cut(s) 358, 569
LweI GCATC 2 cut(s) 30, 1165
MaeI CTAG 4 cut(s) 81, 540, 747, 1019
MaeIII GTNAC 4 cut(s) 15, 48, 495, 1210
MalI GATC 6 cut(s) 588, 705, 777, 822, 855, 1298
MboI GATC 6 cut(s) 586, 703, 775, 820, 853, 1296
MboII GAAGA 8 cut(s) 90, 130, 368, 498, 578, 619, 1016, 1259
MfeI CAATTG 1 cut(s) 648
MhlI GDGCHC 4 cut(s) 219, 245, 1264, 1270
MluCI AATT 6 cut(s) 102, 648, 696, 736, 1029, 1363
MlyI GAGTC 7 cut(s) 79, 245, 503, 514, 682, 883, 1196
MmeI TCCRAC 1 cut(s) 640
MroI TCCGGA 1 cut(s) 1309
MseI TTAA 2 cut(s) 695, 1372
MspA1I CMGCKG 1 cut(s) 901
MspI CCGG 7 cut(s) 158, 213, 227, 392, 796, 1310, 1328
MspR9I CCNGG 3 cut(s) 202, 214, 797
MunI CAATTG 1 cut(s) 648
Mva1269I GAATGC 2 cut(s) 636, 791
MvaI CCWGG 1 cut(s) 202
MvnI CGCG 3 cut(s) 174, 325, 572
MwoI GCNNNNNNNGC 2 cut(s) 40, 331
NciI CCSGG 2 cut(s) 214, 797
NdeII GATC 6 cut(s) 586, 703, 775, 820, 853, 1296
NlaIII CATG 7 cut(s) 193, 323, 533, 593, 943, 1121, 1293
NlaIV GGNNCC 2 cut(s) 217, 231
NmuCI GTSAC 3 cut(s) 15, 48, 495
NspV TTCGAA 1 cut(s) 1002
PauI GCGCGC 1 cut(s) 323
PctI GAATGC 2 cut(s) 636, 791
PfeI GAWTC 8 cut(s) 84, 154, 167, 235, 266, 274, 428, 885
PfoI TCCNGGA 1 cut(s) 795
PkrI GCNGC 5 cut(s) 111, 176, 333, 348, 559
PleI GAGTC 7 cut(s) 78, 245, 502, 513, 681, 882, 1195
PpsI GAGTC 7 cut(s) 78, 245, 502, 513, 681, 882, 1195
PshBI ATTAAT 1 cut(s) 695
Psp124BI GAGCTC 2 cut(s) 245, 1270
Psp6I CCWGG 1 cut(s) 200
PspEI GGTNACC 1 cut(s) 15
PspGI CCWGG 1 cut(s) 200
PspN4I GGNNCC 2 cut(s) 217, 231
PspOMI GGGCCC 1 cut(s) 215
PspPI GGNCC 4 cut(s) 215, 216, 229, 1307
PteI GCGCGC 1 cut(s) 323
PvuII CAGCTG 1 cut(s) 901
RsaI GTAC 4 cut(s) 784, 944, 949, 1223
RsaNI GTAC 4 cut(s) 783, 943, 948, 1222
SacI GAGCTC 2 cut(s) 245, 1270
SaqAI TTAA 2 cut(s) 695, 1372
SatI GCNGC 5 cut(s) 110, 175, 332, 347, 558
Sau3AI GATC 6 cut(s) 586, 703, 775, 820, 853, 1296
Sau96I GGNCC 4 cut(s) 215, 216, 229, 1307
ScaI AGTACT 1 cut(s) 784
SchI GAGTC 7 cut(s) 79, 245, 503, 514, 682, 883, 1196
ScrFI CCNGG 3 cut(s) 202, 214, 797
SduI GDGCHC 4 cut(s) 219, 245, 1264, 1270
SfaNI GCATC 2 cut(s) 30, 1165
SfuI TTCGAA 1 cut(s) 1002
SinI GGWCC 2 cut(s) 229, 1307
SmlI CTYRAG 3 cut(s) 446, 473, 1043
SmoI CTYRAG 3 cut(s) 446, 473, 1043
SpeI ACTAGT 1 cut(s) 539
Sse9I AATT 6 cut(s) 102, 648, 696, 736, 1029, 1363
SsiI CCGC 7 cut(s) 110, 174, 331, 375, 469, 730, 1055
SspMI CTAG 4 cut(s) 81, 540, 747, 1019
SstI GAGCTC 2 cut(s) 245, 1270
StyD4I CCNGG 3 cut(s) 200, 212, 795
TaaI ACNGT 4 cut(s) 30, 715, 947, 1226
TaqI TCGA 3 cut(s) 121, 1002, 1270
TasI AATT 6 cut(s) 102, 648, 696, 736, 1029, 1363
TatI WGTACW 3 cut(s) 782, 942, 947
TauI GCSGC 3 cut(s) 112, 177, 334
TfiI GAWTC 8 cut(s) 84, 154, 167, 235, 266, 274, 428, 885
Tru1I TTAA 2 cut(s) 695, 1372
Tru9I TTAA 2 cut(s) 695, 1372
TscAI CASTG 3 cut(s) 33, 63, 1202
TseFI GTSAC 3 cut(s) 15, 48, 495
TseI GCWGC 2 cut(s) 346, 557
Tsp45I GTSAC 3 cut(s) 15, 48, 495
TspDTI ATGAA 4 cut(s) 442, 531, 756, 1260
TspGWI ACGGA 3 cut(s) 55, 1209, 1221
TspRI CASTG 3 cut(s) 33, 63, 1202
VpaK11BI GGWCC 2 cut(s) 229, 1307
VspI ATTAAT 1 cut(s) 695
XagI CCTNNNNNAGG 1 cut(s) 1073
XapI RAATTY 2 cut(s) 1029, 1363
XbaI TCTAGA 1 cut(s) 1018
XcmI CCANNNNNNNNNTGG 1 cut(s) 898
XmiI GTMKAC 1 cut(s) 816
XspI CTAG 4 cut(s) 81, 540, 747, 1019
ZrmI AGTACT 1 cut(s) 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.