Rh1BG344100

rhodanese-like domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0012988
Physical Location & Seq
Forward (+)
47726206 .. 47728680
2475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG344100.1

Sequence Viewer

Length: 1392 bp
ATGCTAAGGTACAGAGCACCTCCATCACTCTCCCTGAGGATGCTATCTTCTTCTTCACCCTCACTGCACTCCCTAAACCCTAACCCTAAACCTAAATCACACCCCGCCTCTTCAATTCCCGATTCCTCCCCCAATCGCCGTCTTCAACTCTTCTCTAATCCAATTCCAAACCCAATTGGGATTTTTAAACACTGTAACCCTCAAAGTATGACTCTTTCTAGATGCTTGTCCGGGTCGACCAACCCAGTACCCATTTCGAGCTTACCCGAATCGGATGATCCGGATTCGAAAGTTCTGGTCGTGGTCTCCTTCTACAAGTTCGCTGATTTTCCTGACTGTGCTCGTTTGCGGCAACCCTTGAAGCAACTCTGCGAGGAATTGCGCGTTTCAGGTGGGATCATACTTGCACCGGAAGGGATCAATGGCAGCATATGCGGGAGCAGAGAGTCGGTGGAGAAAGTTCTGGAGTTCATTCAAAGTGATGAGCGCCTAAGGGGGCTGAGAAGATTGGAGTCACCTGTGAGTCCGGAAGATGAAGCCATTCATCACGGACACAGTAGCAGTTCTCCTCTGGCAGCAGGGGAGGATGCACCCTTCCGATGGGATCATGTGAGGGTCAAGTTGAAGAAGGAGATTGTTACTCTTGGCATGCCTGCTGTATCACCTACTGAAAAAGTTGGGAAGTATGTGAGTCCAAGTGAATGGAATGAACTGATTAGTGATCCAGATACGGTTGTAATTGATGTGCGCAATAACTATGAAACTAGAATTGGGAAGTTTAAGGGAGCAGTTGATCCGTGTACTAAAGCATTCCGGGAGTTCCCGTCTTGGGTAGAGGATCAGTTCCAACATTCTGAACCGGAGAATGGGCATACAGAAACAAATGCCAACAGTCCAGATGGAAGCACCAAAAATCAACTAGGGATTTCAAAACCAAAAACACCACCACGAGTTGCCATGTACTGTACTGGGGGCATTAGATGTGAGAAAGCTTCTAGTTATCTCCTTAGCAAAGGTTTTGAAGAGGTTTATCATCTAGAAGGTGGGATTCTGAAGTACCTTGAGGAAGTTCCAGAGAAAGAGAGCCTGTGGGAGGGAGAGTGCTTTGTCTTTGACAAGAGAGTCTCTGTTGAGCACGGTTTAGTACAAGGAAATCACAAGTTATGCTATGGGTGTAAGCAACCAGTCAGTGATGCTGATATGGAGTCTCCTCAGTGGGAATGCGGGGTTTCATGTCCCTATTGTTACTCGTCAAAAACCATCGAAGAAAAGGAAAGGGCAAGAGCTCGACAAAGGCAGTTTGAGACATGGGGTATCATTGGCGGTCCAGACAAGGGCCGTAAACCAGATACTATCAAACAGAGGCCTACTCAACTGTCAAATTCAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

463

Amino Acids

51.52

Weight (kDa)

6.65

Isoelectric Point (pI)

54.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0176_N PF17773 100 - 212 2e-22 UPF0176 acylphosphatase like domain
Rhodanese PF00581 232 - 355 2.6e-07 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40760
fragaria_vesca FvH4_7g26040
malus_domestica MD01G1168900.v1.1 MD07G1234800.v1.1
prunus_persica Prupe.2G265100_v2.0.a1
pyrus_communis pycom01g18170 pycom07g21240
rosa_chinensis RchiOBHm_Chr1g0372451
rosa_laevigata RLG00000026897
rosa_multiflora Rmu_sc0011709.1_g000010 Rmu_sc0019374.1_g000001
rosa_roxburghii Rroxscaffold_4G00284910
rosa_rugosa Rorug01G0372000
rosa_samantha Rh1AG380500 Rh1BG344100 Rh1CG357500 Rh1DG375300
rosa_wichuraiana Rw1G033460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1121
Acc16I TGCGCA 1 cut(s) 749
AccI GTMKAC 1 cut(s) 236
AccII CGCG 1 cut(s) 384
AccIII TCCGGA 2 cut(s) 280, 526
AciI CCGC 5 cut(s) 105, 349, 435, 1224, 1323
AclWI GGATC 7 cut(s) 272, 404, 425, 612, 716, 788, 846
AcsI RAATTY 1 cut(s) 1381
AcuI CTGAAG 1 cut(s) 1073
AfaI GTAC 7 cut(s) 11, 249, 802, 962, 967, 1058, 1146
AfiI CCNNNNNNNGG 5 cut(s) 600, 829, 866, 1093, 1334
AgsI TTSAA 8 cut(s) 114, 146, 361, 476, 625, 930, 1022, 1386
AjuI GAANNNNNNNTTGG 2 cut(s) 753, 785
AluBI AGCT 3 cut(s) 261, 992, 1286
AluI AGCT 3 cut(s) 261, 992, 1286
Alw21I GWGCWC 4 cut(s) 19, 343, 1137, 1288
Alw26I GTCTC 4 cut(s) 310, 1129, 1212, 1298
AlwI GGATC 7 cut(s) 272, 404, 425, 612, 716, 788, 846
Aor13HI TCCGGA 2 cut(s) 280, 526
AoxI GGCC 2 cut(s) 1336, 1364
ApeKI GCWGC 2 cut(s) 426, 575
ApoI RAATTY 1 cut(s) 1381
Asp700I GAANNNNTTC 1 cut(s) 540
AspLEI GCGC 3 cut(s) 384, 489, 750
AspS9I GGNCC 2 cut(s) 1325, 1336
AsuC2I CCSGG 2 cut(s) 232, 815
AsuHPI GGTGA 3 cut(s) 48, 507, 654
AsuII TTCGAA 1 cut(s) 287
AvaII GGWCC 1 cut(s) 1325
AxyI CCTNAGG 2 cut(s) 35, 491
BaeI ACNNNNGTAYC 2 cut(s) 720, 753
BanII GRGCYC 1 cut(s) 1288
BauI CACGAG 1 cut(s) 948
BbsI GAAGAC 1 cut(s) 134
Bbv12I GWGCWC 4 cut(s) 19, 343, 1137, 1288
BbvI GCAGC 2 cut(s) 438, 587
BccI CCATC 4 cut(s) 31, 594, 893, 1268
BceAI ACGGC 2 cut(s) 123, 1323
BcnI CCSGG 2 cut(s) 232, 815
BcoDI GTCTC 4 cut(s) 310, 1129, 1212, 1298
BfaI CTAG 5 cut(s) 219, 765, 920, 996, 1037
BfoI RGCGCY 1 cut(s) 490
BisI GCNGC 3 cut(s) 350, 427, 576
BlsI GCNGC 3 cut(s) 351, 428, 577
Bme1390I CCNGG 2 cut(s) 232, 815
Bme18I GGWCC 1 cut(s) 1325
BmgT120I GGNCC 2 cut(s) 1325, 1336
BmrFI CCNGG 2 cut(s) 232, 815
BmrI ACTGGG 2 cut(s) 239, 978
BmsI GCATC 4 cut(s) 30, 212, 577, 1183
BmuI ACTGGG 2 cut(s) 239, 978
BpiI GAAGAC 1 cut(s) 134
BplI GAGNNNNNCTC 2 cut(s) 1354, 1386
BpmI CTGGAG 1 cut(s) 485
Bpu10I CCTNAGC 2 cut(s) 5, 1007
Bpu14I TTCGAA 1 cut(s) 287
BpuEI CTTGAG 1 cut(s) 1082
BpuMI CCSGG 2 cut(s) 232, 815
BsaI GGTCTC 1 cut(s) 310
BsaWI WCCGGW 4 cut(s) 280, 409, 526, 859
BsaXI ACNNNNNCTCC 4 cut(s) 809, 839, 1085, 1115
Bsc4I CCNNNNNNNGG 5 cut(s) 600, 829, 866, 1093, 1334
Bse1I ACTGG 3 cut(s) 245, 973, 1184
Bse21I CCTNAGG 2 cut(s) 35, 491
BseAI TCCGGA 2 cut(s) 280, 526
BseGI GGATG 3 cut(s) 45, 280, 592
BseLI CCNNNNNNNGG 5 cut(s) 600, 829, 866, 1093, 1334
BseMII CTCAG 3 cut(s) 26, 491, 1226
BseNI ACTGG 3 cut(s) 245, 973, 1184
BseRI GAGGAG 2 cut(s) 558, 1200
BseXI GCAGC 2 cut(s) 438, 587
BsgI GTGCAG 1 cut(s) 50
Bsh1236I CGCG 1 cut(s) 384
BshFI GGCC 2 cut(s) 1338, 1366
BsiHKAI GWGCWC 4 cut(s) 19, 343, 1137, 1288
BsiSI CCGG 6 cut(s) 231, 281, 410, 527, 814, 860
BslFI GGGAC 1 cut(s) 1221
BslI CCNNNNNNNGG 5 cut(s) 600, 829, 866, 1093, 1334
BsmAI GTCTC 4 cut(s) 310, 1129, 1212, 1298
BsmFI GGGAC 1 cut(s) 1221
BsmI GAATGC 2 cut(s) 809, 1226
BsnI GGCC 2 cut(s) 1338, 1366
Bso31I GGTCTC 1 cut(s) 310
Bsp119I TTCGAA 1 cut(s) 287
Bsp1286I GDGCHC 4 cut(s) 19, 343, 1137, 1288
Bsp13I TCCGGA 2 cut(s) 280, 526
Bsp143I GATC 7 cut(s) 277, 396, 417, 604, 721, 793, 838
BspACI CCGC 5 cut(s) 105, 349, 435, 1224, 1323
BspANI GGCC 2 cut(s) 1338, 1366
BspCNI CTCAG 3 cut(s) 27, 492, 1225
BspEI TCCGGA 2 cut(s) 280, 526
BspFNI CGCG 1 cut(s) 384
BspPI GGATC 7 cut(s) 272, 404, 425, 612, 716, 788, 846
BspT104I TTCGAA 1 cut(s) 287
BspTNI GGTCTC 1 cut(s) 310
BsrI ACTGG 3 cut(s) 245, 973, 1184
BssMI GATC 7 cut(s) 277, 396, 417, 604, 721, 793, 838
BssSI CACGAG 1 cut(s) 948
Bst2BI CACGAG 1 cut(s) 948
Bst4CI ACNGT 8 cut(s) 194, 338, 557, 733, 893, 965, 1139, 1377
Bst6I CTCTTC 3 cut(s) 115, 155, 1017
BstAPI GCANNNNNTGC 1 cut(s) 432
BstBI TTCGAA 1 cut(s) 287
BstC8I GCNNGC 2 cut(s) 650, 654
BstDEI CTNAG 6 cut(s) 5, 35, 491, 500, 1007, 1212
BstENI CCTNNNNNAGG 1 cut(s) 1091
BstF5I GGATG 3 cut(s) 45, 280, 592
BstFNI CGCG 1 cut(s) 384
BstH2I RGCGCY 1 cut(s) 490
BstHHI GCGC 3 cut(s) 384, 489, 750
BstKTI GATC 7 cut(s) 280, 399, 420, 607, 724, 796, 841
BstMAI GTCTC 4 cut(s) 310, 1129, 1212, 1298
BstMBI GATC 7 cut(s) 277, 396, 417, 604, 721, 793, 838
BstMWI GCNNNNNNNGC 1 cut(s) 432
BstNSI RCATGY 1 cut(s) 652
BstSCI CCNGG 2 cut(s) 230, 813
BstUI CGCG 1 cut(s) 384
BstV1I GCAGC 2 cut(s) 438, 587
BstV2I GAAGAC 1 cut(s) 134
BstXI CCANNNNNNTGG 1 cut(s) 702
Bsu36I CCTNAGG 2 cut(s) 35, 491
BsuRI GGCC 2 cut(s) 1338, 1366
BtsCI GGATG 3 cut(s) 45, 280, 592
BtsI GCAGTG 1 cut(s) 62
BtsIMutI CAGTG 4 cut(s) 62, 190, 1195, 1220
Cac8I GCNNGC 2 cut(s) 650, 654
CfoI GCGC 3 cut(s) 384, 489, 750
Cfr13I GGNCC 2 cut(s) 1325, 1336
Csp6I GTAC 7 cut(s) 10, 248, 801, 961, 966, 1057, 1145
CviAII CATG 5 cut(s) 608, 649, 958, 1233, 1308
CviJI RGCY 8 cut(s) 261, 499, 539, 992, 1086, 1286, 1338, 1366
CviKI_1 RGCY 8 cut(s) 261, 499, 539, 992, 1086, 1286, 1338, 1366
CviQI GTAC 7 cut(s) 10, 248, 801, 961, 966, 1057, 1145
DdeI CTNAG 6 cut(s) 5, 35, 491, 500, 1007, 1212
DpnI GATC 7 cut(s) 279, 398, 419, 606, 723, 795, 840
DpnII GATC 7 cut(s) 277, 396, 417, 604, 721, 793, 838
DraI TTTAAA 1 cut(s) 187
DrdI GACNNNNNNGTC 1 cut(s) 1121
DseDI GACNNNNNNGTC 1 cut(s) 1121
Eam1104I CTCTTC 3 cut(s) 115, 155, 1017
EarI CTCTTC 3 cut(s) 115, 155, 1017
Ecl136II GAGCTC 1 cut(s) 1286
Eco147I AGGCCT 1 cut(s) 1366
Eco24I GRGCYC 1 cut(s) 1288
Eco31I GGTCTC 1 cut(s) 310
Eco47I GGWCC 1 cut(s) 1325
Eco53kI GAGCTC 1 cut(s) 1286
Eco57I CTGAAG 1 cut(s) 1073
Eco81I CCTNAGG 2 cut(s) 35, 491
EcoICRI GAGCTC 1 cut(s) 1286
EcoNI CCTNNNNNAGG 1 cut(s) 1091
EcoT38I GRGCYC 1 cut(s) 1288
FaeI CATG 5 cut(s) 611, 652, 961, 1236, 1311
FaqI GGGAC 1 cut(s) 1221
FatI CATG 5 cut(s) 607, 648, 957, 1232, 1307
FauI CCCGC 3 cut(s) 112, 428, 1217
FauNDI CATATG 1 cut(s) 431
FblI GTMKAC 1 cut(s) 236
Fnu4HI GCNGC 3 cut(s) 350, 427, 576
FokI GGATG 3 cut(s) 52, 287, 599
FriOI GRGCYC 1 cut(s) 1288
Fsp4HI GCNGC 3 cut(s) 350, 427, 576
FspBI CTAG 5 cut(s) 219, 765, 920, 996, 1037
FspI TGCGCA 1 cut(s) 749
GlaI GCGC 3 cut(s) 383, 488, 749
GluI GCNGC 3 cut(s) 350, 427, 576
GsuI CTGGAG 1 cut(s) 485
HaeII RGCGCY 1 cut(s) 490
HaeIII GGCC 2 cut(s) 1338, 1366
HapII CCGG 6 cut(s) 231, 281, 410, 527, 814, 860
HhaI GCGC 3 cut(s) 384, 489, 750
Hin1II CATG 5 cut(s) 611, 652, 961, 1236, 1311
Hin6I GCGC 3 cut(s) 382, 487, 748
HinP1I GCGC 3 cut(s) 382, 487, 748
HincII GTYRAC 1 cut(s) 237
HindII GTYRAC 1 cut(s) 237
HindIII AAGCTT 1 cut(s) 990
HpaII CCGG 6 cut(s) 231, 281, 410, 527, 814, 860
HphI GGTGA 3 cut(s) 48, 507, 654
Hpy166II GTNNAC 3 cut(s) 237, 801, 1343
Hpy188I TCNGA 4 cut(s) 274, 599, 856, 1053
Hpy8I GTNNAC 3 cut(s) 237, 801, 1343
HpyAV CCTTC 5 cut(s) 319, 407, 604, 622, 1034
HpyCH4III ACNGT 8 cut(s) 194, 338, 557, 733, 893, 965, 1139, 1377
HpyCH4V TGCA 3 cut(s) 67, 407, 590
HpyF10VI GCNNNNNNNGC 1 cut(s) 432
HpyF3I CTNAG 6 cut(s) 5, 35, 491, 500, 1007, 1212
Hsp92II CATG 5 cut(s) 611, 652, 961, 1236, 1311
HspAI GCGC 3 cut(s) 382, 487, 748
Kpn2I TCCGGA 2 cut(s) 280, 526
Kzo9I GATC 7 cut(s) 277, 396, 417, 604, 721, 793, 838
LmnI GCTCC 2 cut(s) 438, 785
Lsp1109I GCAGC 2 cut(s) 438, 587
LweI GCATC 4 cut(s) 30, 212, 577, 1183
MaeI CTAG 5 cut(s) 219, 765, 920, 996, 1037
MaeIII GTNAC 4 cut(s) 194, 513, 637, 1244
MalI GATC 7 cut(s) 279, 398, 419, 606, 723, 795, 840
MboI GATC 7 cut(s) 277, 396, 417, 604, 721, 793, 838
MfeI CAATTG 1 cut(s) 174
MhlI GDGCHC 4 cut(s) 19, 343, 1137, 1288
MluCI AATT 8 cut(s) 114, 162, 174, 377, 738, 768, 1381, 1386
MlyI GAGTC 7 cut(s) 205, 455, 521, 532, 700, 1131, 1214
MmeI TCCRAC 1 cut(s) 871
MroI TCCGGA 2 cut(s) 280, 526
MroXI GAANNNNTTC 1 cut(s) 540
MseI TTAA 2 cut(s) 186, 780
MspI CCGG 6 cut(s) 231, 281, 410, 527, 814, 860
MspR9I CCNGG 2 cut(s) 232, 815
MunI CAATTG 1 cut(s) 174
Mva1269I GAATGC 2 cut(s) 809, 1226
MvnI CGCG 1 cut(s) 384
MwoI GCNNNNNNNGC 1 cut(s) 432
NciI CCSGG 2 cut(s) 232, 815
NdeI CATATG 1 cut(s) 431
NdeII GATC 7 cut(s) 277, 396, 417, 604, 721, 793, 838
NlaIII CATG 5 cut(s) 611, 652, 961, 1236, 1311
NmuCI GTSAC 1 cut(s) 513
NsbI TGCGCA 1 cut(s) 749
NspI RCATGY 1 cut(s) 652
NspV TTCGAA 1 cut(s) 287
PaeI GCATGC 1 cut(s) 652
PceI AGGCCT 1 cut(s) 1366
PctI GAATGC 2 cut(s) 809, 1226
PdmI GAANNNNTTC 1 cut(s) 540
PfeI GAWTC 4 cut(s) 122, 269, 284, 1048
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 3 cut(s) 351, 428, 577
PleI GAGTC 7 cut(s) 205, 454, 520, 531, 699, 1130, 1213
PpsI GAGTC 7 cut(s) 205, 454, 520, 531, 699, 1130, 1213
Psp124BI GAGCTC 1 cut(s) 1288
PspPI GGNCC 2 cut(s) 1325, 1336
RsaI GTAC 7 cut(s) 11, 249, 802, 962, 967, 1058, 1146
RsaNI GTAC 7 cut(s) 10, 248, 801, 961, 966, 1057, 1145
SacI GAGCTC 1 cut(s) 1288
SalI GTCGAC 1 cut(s) 235
SaqAI TTAA 2 cut(s) 186, 780
SatI GCNGC 3 cut(s) 350, 427, 576
Sau3AI GATC 7 cut(s) 277, 396, 417, 604, 721, 793, 838
Sau96I GGNCC 2 cut(s) 1325, 1336
SchI GAGTC 7 cut(s) 205, 455, 521, 532, 700, 1131, 1214
ScrFI CCNGG 2 cut(s) 232, 815
SduI GDGCHC 4 cut(s) 19, 343, 1137, 1288
SfaNI GCATC 4 cut(s) 30, 212, 577, 1183
SfuI TTCGAA 1 cut(s) 287
SinI GGWCC 1 cut(s) 1325
SmlI CTYRAG 1 cut(s) 1061
SmoI CTYRAG 1 cut(s) 1061
SphI GCATGC 1 cut(s) 652
Sse9I AATT 8 cut(s) 114, 162, 174, 377, 738, 768, 1381, 1386
SseBI AGGCCT 1 cut(s) 1366
SsiI CCGC 5 cut(s) 105, 349, 435, 1224, 1323
SspMI CTAG 5 cut(s) 219, 765, 920, 996, 1037
SstI GAGCTC 1 cut(s) 1288
StuI AGGCCT 1 cut(s) 1366
StyD4I CCNGG 2 cut(s) 230, 813
TaaI ACNGT 8 cut(s) 194, 338, 557, 733, 893, 965, 1139, 1377
TaqI TCGA 5 cut(s) 236, 257, 287, 1263, 1288
TasI AATT 8 cut(s) 114, 162, 174, 377, 738, 768, 1381, 1386
TatI WGTACW 4 cut(s) 800, 960, 965, 1144
TauI GCSGC 1 cut(s) 352
TfiI GAWTC 4 cut(s) 122, 269, 284, 1048
Tru1I TTAA 2 cut(s) 186, 780
Tru9I TTAA 2 cut(s) 186, 780
TscAI CASTG 4 cut(s) 69, 197, 1195, 1220
TseFI GTSAC 1 cut(s) 513
TseI GCWGC 2 cut(s) 426, 575
Tsp45I GTSAC 1 cut(s) 513
TspDTI ATGAA 6 cut(s) 460, 533, 549, 723, 774, 1221
TspGWI ACGGA 2 cut(s) 564, 786
TspRI CASTG 4 cut(s) 69, 197, 1195, 1220
VpaK11BI GGWCC 1 cut(s) 1325
XagI CCTNNNNNAGG 1 cut(s) 1091
XapI RAATTY 1 cut(s) 1381
XbaI TCTAGA 2 cut(s) 218, 1036
XceI RCATGY 1 cut(s) 652
XmiI GTMKAC 1 cut(s) 236
XmnI GAANNNNTTC 1 cut(s) 540
XspI CTAG 5 cut(s) 219, 765, 920, 996, 1037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.