FvH4_7g26040

rhodanese-like domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0012988
Physical Location & Seq
Forward (+)
19788549 .. 19791862
3314 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g26040.t1

Sequence Viewer

Length: 1419 bp
ATGCTTAGGTACAGAGCCCCTCCGTCACTCTCCCTGAGAATGCTAACGTCACCGTCACTTCAATCCCTAAACCCTAACCCTAAATCACACCCCACCTCTTCAATTTCAAATTCCTCCCCCAACCGCCGCCGACTTCAGCTCTTCTCTAATCCGATTCCAAATCCAGTTGGGATTTCTCACTGTAACCCTCAAAGTATGACACTTTCGAGATGCTTATCCGGGTCAACCGACCCGGTTCCCATTTCGAACTTACCCGAATCCGATTCCGATTCGAATGTTCTGGTGGTGGTCTCCTTCTACAAGTTCGCTGATTTTCCTGACTCTGCTCGTTTGCGGCAACCCTTGAAGCAGCTCTGCGAGGAATTGCGCGTTTCAGGCGGGATCATACTTGCGCCTGAAGGGATCAATGGCAGCATATGCGGGAGCAGAGAGTCGGTGGAGAATGTTCTTGAGTTCATTCAGAGTGATGATCGTCTCAGGGGGCTAAGAAGATTGGAGTCGCCTGTGAGTCCGGAGGATGAAGCTATTCATCATGGACACAGTAGCAGTTCTCCTCTGGCAGCAGGGGAAGATGCGCCCTTCCGATGGGATCATGTGAGGGTCAAGTTGAAGAAGGAGATTGTTACTCTTGGCATGCCTGCTGTATCACCTACTGAAAAGGTTGGGAAGTATGTGAGTCCAAGTGAATGGAATGAACTGATTAGTGATCCAGATACAGTCGTAATTGATGTGCGTAATAACTATGAAACTAGAATTGGGAAGTTCAAGGGAGCAGTTGATCCATGTACAAAAGCATTCCGGGAGTTCCCATCTTGGGTAGAGGACCAGTTCCAACCTTCTGAACCTGAAAATGGGCATACAGAAGCAAATGCCAACAGTTCGGATGGAAGCACCAAAAATCAACCAGAGATTTCAAAACCAAAAACACCACCGCGAGTTGCCATGTACTGTACCGGGGGAATTAGATGTGAGAAAGCTTCTAGTTATCTTCTCAGCAAAGGTTTCGAAGAGGTTTATCATCTAGAAGGCGGGATTCTGAAGTATCTTGAAGAAGTTCCGGAGAAAGAGAGCCTGTGGAAGGGAGAGTGTTTTGTCTTTGACAAGCGAGTCTCCGTCGAGCATGGTTTAGTACAAGGAAATCACAAGTTATGCTATGGGTGCAAGCAGCCCGTCAGTGATGCTGATATGGAGTCTCCTCAGTGGGAGTACGGGGTTTCTTGTCCCTATTGCTACTCGTCAAAATCTCTCGAAGAGAAGGAAAGGGCAAGAGCTCGACAAAGGCAATTTGAGACATGGGGCATCATCGGCGGTCCAGACAAGGGTCGTAAGCCAGATACTATTAAACAGAACCAAACTACTCAAAGTGTCGAATTCAATTTAGATGGAGACTTGCAAAAGGAATCTCGTGTCAAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

473

Amino Acids

52.63

Weight (kDa)

6.13

Isoelectric Point (pI)

58.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0176_N PF17773 95 - 207 8.3e-22 UPF0176 acylphosphatase like domain
Rhodanese PF00581 227 - 350 1.4e-07 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40760
fragaria_vesca FvH4_7g26040
malus_domestica MD01G1168900.v1.1 MD07G1234800.v1.1
prunus_persica Prupe.2G265100_v2.0.a1
pyrus_communis pycom01g18170 pycom07g21240
rosa_chinensis RchiOBHm_Chr1g0372451
rosa_laevigata RLG00000026897
rosa_multiflora Rmu_sc0011709.1_g000010 Rmu_sc0019374.1_g000001
rosa_roxburghii Rroxscaffold_4G00284910
rosa_rugosa Rorug01G0372000
rosa_samantha Rh1AG380500 Rh1BG344100 Rh1CG357500 Rh1DG375300
rosa_wichuraiana Rw1G033460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1106
AccII CGCG 2 cut(s) 369, 934
AccIII TCCGGA 2 cut(s) 511, 1057
AciI CCGC 8 cut(s) 124, 127, 334, 378, 420, 932, 1029, 1308
AclWI GGATC 5 cut(s) 389, 410, 597, 701, 773
AcsI RAATTY 2 cut(s) 109, 1370
AcuI CTGAAG 3 cut(s) 119, 417, 1058
AfaI GTAC 6 cut(s) 11, 787, 947, 952, 1131, 1208
AfiI CCNNNNNNNGG 5 cut(s) 585, 814, 851, 1078, 1319
AgsI TTSAA 9 cut(s) 62, 102, 108, 346, 610, 766, 915, 1049, 1375
AjuI GAANNNNNNNTTGG 2 cut(s) 738, 770
AluBI AGCT 5 cut(s) 139, 352, 524, 977, 1271
AluI AGCT 5 cut(s) 139, 352, 524, 977, 1271
Alw21I GWGCWC 1 cut(s) 1273
Alw26I GTCTC 6 cut(s) 295, 479, 1114, 1197, 1283, 1380
AlwI GGATC 5 cut(s) 389, 410, 597, 701, 773
Aor13HI TCCGGA 2 cut(s) 511, 1057
ApeKI GCWGC 4 cut(s) 349, 411, 560, 1165
ApoI RAATTY 2 cut(s) 109, 1370
Asp700I GAANNNNTTC 2 cut(s) 525, 1053
AspLEI GCGC 3 cut(s) 369, 394, 577
AspS9I GGNCC 2 cut(s) 823, 1310
AsuC2I CCSGG 4 cut(s) 220, 233, 800, 955
AsuHPI GGTGA 2 cut(s) 42, 639
AsuII TTCGAA 3 cut(s) 245, 272, 1005
AvaII GGWCC 2 cut(s) 823, 1310
BaeI ACNNNNGTAYC 2 cut(s) 705, 738
BanII GRGCYC 2 cut(s) 19, 1273
BauI CACGAG 1 cut(s) 1404
Bbv12I GWGCWC 1 cut(s) 1273
BbvI GCAGC 4 cut(s) 361, 423, 572, 1177
BccI CCATC 4 cut(s) 579, 817, 878, 1376
BcgI CGANNNNNNTGC 2 cut(s) 985, 1019
BcnI CCSGG 4 cut(s) 220, 233, 800, 955
BcoDI GTCTC 6 cut(s) 295, 479, 1114, 1197, 1283, 1380
BfaI CTAG 3 cut(s) 750, 981, 1022
BisI GCNGC 6 cut(s) 127, 335, 350, 412, 561, 1166
BlsI GCNGC 6 cut(s) 128, 336, 351, 413, 562, 1167
Bme1390I CCNGG 4 cut(s) 220, 233, 800, 955
Bme18I GGWCC 2 cut(s) 823, 1310
BmgT120I GGNCC 2 cut(s) 823, 1310
BmiI GGNNCC 1 cut(s) 237
BmrFI CCNGG 4 cut(s) 220, 233, 800, 955
BmsI GCATC 4 cut(s) 200, 562, 1168, 1308
BoxI GACNNNNGTC 1 cut(s) 1320
Bpu10I CCTNAGC 1 cut(s) 5
Bpu14I TTCGAA 3 cut(s) 245, 272, 1005
BpuEI CTTGAG 1 cut(s) 470
BpuMI CCSGG 4 cut(s) 220, 233, 800, 955
BsaBI GATNNNNATC 1 cut(s) 214
BsaI GGTCTC 1 cut(s) 295
BsaJI CCNNGG 1 cut(s) 954
BsaWI WCCGGW 2 cut(s) 511, 1057
Bsc4I CCNNNNNNNGG 5 cut(s) 585, 814, 851, 1078, 1319
Bse1I ACTGG 2 cut(s) 164, 826
Bse8I GATNNNNATC 1 cut(s) 214
BseAI TCCGGA 2 cut(s) 511, 1057
BseDI CCNNGG 1 cut(s) 954
BseGI GGATG 2 cut(s) 523, 889
BseJI GATNNNNATC 1 cut(s) 214
BseLI CCNNNNNNNGG 5 cut(s) 585, 814, 851, 1078, 1319
BseMII CTCAG 4 cut(s) 26, 490, 1006, 1211
BseNI ACTGG 2 cut(s) 164, 826
BseRI GAGGAG 2 cut(s) 543, 1185
BseXI GCAGC 4 cut(s) 361, 423, 572, 1177
Bsh1236I CGCG 2 cut(s) 369, 934
BsiHKAI GWGCWC 1 cut(s) 1273
BsiSI CCGG 6 cut(s) 219, 233, 512, 799, 954, 1058
BslFI GGGAC 1 cut(s) 1206
BslI CCNNNNNNNGG 5 cut(s) 585, 814, 851, 1078, 1319
BsmAI GTCTC 6 cut(s) 295, 479, 1114, 1197, 1283, 1380
BsmBI CGTCTC 1 cut(s) 479
BsmFI GGGAC 1 cut(s) 1206
BsmI GAATGC 2 cut(s) 45, 794
Bso31I GGTCTC 1 cut(s) 295
Bsp119I TTCGAA 3 cut(s) 245, 272, 1005
Bsp1286I GDGCHC 2 cut(s) 19, 1273
Bsp13I TCCGGA 2 cut(s) 511, 1057
Bsp1407I TGTACA 1 cut(s) 785
Bsp143I GATC 6 cut(s) 381, 402, 469, 589, 706, 778
BspACI CCGC 8 cut(s) 124, 127, 334, 378, 420, 932, 1029, 1308
BspCNI CTCAG 4 cut(s) 27, 489, 1005, 1210
BspEI TCCGGA 2 cut(s) 511, 1057
BspFNI CGCG 2 cut(s) 369, 934
BspLI GGNNCC 1 cut(s) 237
BspPI GGATC 5 cut(s) 389, 410, 597, 701, 773
BspQI GCTCTTC 1 cut(s) 146
BspT104I TTCGAA 3 cut(s) 245, 272, 1005
BspTNI GGTCTC 1 cut(s) 295
BsrGI TGTACA 1 cut(s) 785
BsrI ACTGG 2 cut(s) 164, 826
BssECI CCNNGG 1 cut(s) 954
BssMI GATC 6 cut(s) 381, 402, 469, 589, 706, 778
BssSI CACGAG 1 cut(s) 1404
Bst2BI CACGAG 1 cut(s) 1404
Bst4CI ACNGT 6 cut(s) 54, 182, 542, 718, 878, 950
Bst6I CTCTTC 4 cut(s) 103, 146, 1002, 1245
BstAPI GCANNNNNTGC 1 cut(s) 417
BstAUI TGTACA 1 cut(s) 785
BstBI TTCGAA 3 cut(s) 245, 272, 1005
BstC8I GCNNGC 3 cut(s) 635, 639, 1163
BstDEI CTNAG 6 cut(s) 5, 35, 476, 485, 992, 1197
BstENI CCTNNNNNAGG 1 cut(s) 1076
BstF5I GGATG 2 cut(s) 523, 889
BstFNI CGCG 2 cut(s) 369, 934
BstHHI GCGC 3 cut(s) 369, 394, 577
BstKTI GATC 6 cut(s) 384, 405, 472, 592, 709, 781
BstMAI GTCTC 6 cut(s) 295, 479, 1114, 1197, 1283, 1380
BstMBI GATC 6 cut(s) 381, 402, 469, 589, 706, 778
BstMWI GCNNNNNNNGC 4 cut(s) 375, 417, 1158, 1305
BstNSI RCATGY 1 cut(s) 637
BstPAI GACNNNNGTC 1 cut(s) 1320
BstSCI CCNGG 4 cut(s) 218, 231, 798, 953
BstUI CGCG 2 cut(s) 369, 934
BstV1I GCAGC 4 cut(s) 361, 423, 572, 1177
BstXI CCANNNNNNTGG 1 cut(s) 687
BtsCI GGATG 2 cut(s) 523, 889
BtsIMutI CAGTG 3 cut(s) 178, 1180, 1205
Cac8I GCNNGC 3 cut(s) 635, 639, 1163
CfoI GCGC 3 cut(s) 369, 394, 577
Cfr13I GGNCC 2 cut(s) 823, 1310
Csp6I GTAC 6 cut(s) 10, 786, 946, 951, 1130, 1207
CviAII CATG 7 cut(s) 533, 593, 634, 783, 943, 1121, 1293
CviQI GTAC 6 cut(s) 10, 786, 946, 951, 1130, 1207
DdeI CTNAG 6 cut(s) 5, 35, 476, 485, 992, 1197
DpnI GATC 6 cut(s) 383, 404, 471, 591, 708, 780
DpnII GATC 6 cut(s) 381, 402, 469, 589, 706, 778
DrdI GACNNNNNNGTC 1 cut(s) 1106
DseDI GACNNNNNNGTC 1 cut(s) 1106
Eam1104I CTCTTC 4 cut(s) 103, 146, 1002, 1245
EarI CTCTTC 4 cut(s) 103, 146, 1002, 1245
Ecl136II GAGCTC 1 cut(s) 1271
Eco24I GRGCYC 2 cut(s) 19, 1273
Eco31I GGTCTC 1 cut(s) 295
Eco47I GGWCC 2 cut(s) 823, 1310
Eco53kI GAGCTC 1 cut(s) 1271
Eco57I CTGAAG 3 cut(s) 119, 417, 1058
EcoICRI GAGCTC 1 cut(s) 1271
EcoNI CCTNNNNNAGG 1 cut(s) 1076
EcoRI GAATTC 1 cut(s) 1370
EcoT38I GRGCYC 2 cut(s) 19, 1273
Esp3I CGTCTC 1 cut(s) 479
FaeI CATG 7 cut(s) 536, 596, 637, 786, 946, 1124, 1296
FaqI GGGAC 1 cut(s) 1206
FatI CATG 7 cut(s) 532, 592, 633, 782, 942, 1120, 1292
FauI CCCGC 3 cut(s) 371, 413, 1022
FauNDI CATATG 1 cut(s) 416
Fnu4HI GCNGC 6 cut(s) 127, 335, 350, 412, 561, 1166
FokI GGATG 2 cut(s) 530, 896
FriOI GRGCYC 2 cut(s) 19, 1273
Fsp4HI GCNGC 6 cut(s) 127, 335, 350, 412, 561, 1166
FspBI CTAG 3 cut(s) 750, 981, 1022
GlaI GCGC 3 cut(s) 368, 393, 576
GluI GCNGC 6 cut(s) 127, 335, 350, 412, 561, 1166
HapII CCGG 6 cut(s) 219, 233, 512, 799, 954, 1058
HhaI GCGC 3 cut(s) 369, 394, 577
Hin1II CATG 7 cut(s) 536, 596, 637, 786, 946, 1124, 1296
Hin6I GCGC 3 cut(s) 367, 392, 575
HinP1I GCGC 3 cut(s) 367, 392, 575
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HindIII AAGCTT 1 cut(s) 975
HpaII CCGG 6 cut(s) 219, 233, 512, 799, 954, 1058
HphI GGTGA 2 cut(s) 42, 639
Hpy166II GTNNAC 1 cut(s) 225
Hpy188I TCNGA 8 cut(s) 153, 262, 268, 462, 584, 841, 883, 1038
Hpy8I GTNNAC 1 cut(s) 225
Hpy99I CGWCG 1 cut(s) 1118
HpyAV CCTTC 8 cut(s) 304, 392, 589, 607, 846, 1019, 1072, 1249
HpyCH4III ACNGT 6 cut(s) 54, 182, 542, 718, 878, 950
HpyCH4IV ACGT 1 cut(s) 47
HpyCH4V TGCA 2 cut(s) 1161, 1393
HpyF10VI GCNNNNNNNGC 4 cut(s) 375, 417, 1158, 1305
HpyF3I CTNAG 6 cut(s) 5, 35, 476, 485, 992, 1197
HpySE526I ACGT 1 cut(s) 47
Hsp92II CATG 7 cut(s) 536, 596, 637, 786, 946, 1124, 1296
HspAI GCGC 3 cut(s) 367, 392, 575
Kpn2I TCCGGA 2 cut(s) 511, 1057
Kzo9I GATC 6 cut(s) 381, 402, 469, 589, 706, 778
LguI GCTCTTC 1 cut(s) 146
LmnI GCTCC 2 cut(s) 423, 770
Lsp1109I GCAGC 4 cut(s) 361, 423, 572, 1177
LweI GCATC 4 cut(s) 200, 562, 1168, 1308
MaeI CTAG 3 cut(s) 750, 981, 1022
MaeII ACGT 1 cut(s) 47
MaeIII GTNAC 5 cut(s) 24, 48, 54, 182, 622
MalI GATC 6 cut(s) 383, 404, 471, 591, 708, 780
MboI GATC 6 cut(s) 381, 402, 469, 589, 706, 778
MboII GAAGA 9 cut(s) 90, 133, 501, 581, 622, 980, 1019, 1061, 1262
MhlI GDGCHC 2 cut(s) 19, 1273
MluCI AATT 9 cut(s) 102, 109, 362, 723, 753, 960, 1283, 1370, 1375
MlyI GAGTC 7 cut(s) 314, 440, 506, 517, 685, 1116, 1199
MmeI TCCRAC 1 cut(s) 856
MroI TCCGGA 2 cut(s) 511, 1057
MroXI GAANNNNTTC 2 cut(s) 525, 1053
MseI TTAA 1 cut(s) 1341
MspI CCGG 6 cut(s) 219, 233, 512, 799, 954, 1058
MspR9I CCNGG 4 cut(s) 220, 233, 800, 955
Mva1269I GAATGC 2 cut(s) 45, 794
MvnI CGCG 2 cut(s) 369, 934
MwoI GCNNNNNNNGC 4 cut(s) 375, 417, 1158, 1305
NciI CCSGG 4 cut(s) 220, 233, 800, 955
NdeI CATATG 1 cut(s) 416
NdeII GATC 6 cut(s) 381, 402, 469, 589, 706, 778
NlaIII CATG 7 cut(s) 536, 596, 637, 786, 946, 1124, 1296
NlaIV GGNNCC 1 cut(s) 237
NmuCI GTSAC 3 cut(s) 24, 48, 54
NspI RCATGY 1 cut(s) 637
NspV TTCGAA 3 cut(s) 245, 272, 1005
PaeI GCATGC 1 cut(s) 637
PciSI GCTCTTC 1 cut(s) 146
PctI GAATGC 2 cut(s) 45, 794
PdmI GAANNNNTTC 2 cut(s) 525, 1053
PfeI GAWTC 6 cut(s) 154, 257, 263, 269, 1033, 1400
PfoI TCCNGGA 1 cut(s) 798
PkrI GCNGC 6 cut(s) 128, 336, 351, 413, 562, 1167
PleI GAGTC 7 cut(s) 314, 439, 505, 516, 684, 1115, 1198
PpsI GAGTC 7 cut(s) 314, 439, 505, 516, 684, 1115, 1198
PshAI GACNNNNGTC 1 cut(s) 1320
Psp124BI GAGCTC 1 cut(s) 1273
PspN4I GGNNCC 1 cut(s) 237
PspPI GGNCC 2 cut(s) 823, 1310
RsaI GTAC 6 cut(s) 11, 787, 947, 952, 1131, 1208
RsaNI GTAC 6 cut(s) 10, 786, 946, 951, 1130, 1207
SacI GAGCTC 1 cut(s) 1273
SapI GCTCTTC 1 cut(s) 146
SaqAI TTAA 1 cut(s) 1341
SatI GCNGC 6 cut(s) 127, 335, 350, 412, 561, 1166
Sau3AI GATC 6 cut(s) 381, 402, 469, 589, 706, 778
Sau96I GGNCC 2 cut(s) 823, 1310
SchI GAGTC 7 cut(s) 314, 440, 506, 517, 685, 1116, 1199
ScrFI CCNGG 4 cut(s) 220, 233, 800, 955
SduI GDGCHC 2 cut(s) 19, 1273
SfaNI GCATC 4 cut(s) 200, 562, 1168, 1308
SfuI TTCGAA 3 cut(s) 245, 272, 1005
SinI GGWCC 2 cut(s) 823, 1310
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
SphI GCATGC 1 cut(s) 637
Sse9I AATT 9 cut(s) 102, 109, 362, 723, 753, 960, 1283, 1370, 1375
SsiI CCGC 8 cut(s) 124, 127, 334, 378, 420, 932, 1029, 1308
SspMI CTAG 3 cut(s) 750, 981, 1022
SstI GAGCTC 1 cut(s) 1273
StyD4I CCNGG 4 cut(s) 218, 231, 798, 953
TaaI ACNGT 6 cut(s) 54, 182, 542, 718, 878, 950
TaiI ACGT 1 cut(s) 50
TaqI TCGA 8 cut(s) 206, 245, 272, 1005, 1116, 1248, 1273, 1368
TasI AATT 9 cut(s) 102, 109, 362, 723, 753, 960, 1283, 1370, 1375
TatI WGTACW 3 cut(s) 785, 945, 1129
TauI GCSGC 2 cut(s) 129, 337
TfiI GAWTC 6 cut(s) 154, 257, 263, 269, 1033, 1400
Tru1I TTAA 1 cut(s) 1341
Tru9I TTAA 1 cut(s) 1341
TscAI CASTG 3 cut(s) 185, 1180, 1205
TseFI GTSAC 3 cut(s) 24, 48, 54
TseI GCWGC 4 cut(s) 349, 411, 560, 1165
Tsp45I GTSAC 3 cut(s) 24, 48, 54
TspDTI ATGAA 5 cut(s) 445, 518, 534, 708, 759
TspGWI ACGGA 2 cut(s) 12, 1102
TspRI CASTG 3 cut(s) 185, 1180, 1205
VpaK11BI GGWCC 2 cut(s) 823, 1310
XagI CCTNNNNNAGG 1 cut(s) 1076
XapI RAATTY 2 cut(s) 109, 1370
XbaI TCTAGA 1 cut(s) 1021
XceI RCATGY 1 cut(s) 637
XmnI GAANNNNTTC 2 cut(s) 525, 1053
XspI CTAG 3 cut(s) 750, 981, 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.