AT2G42170

Actin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008204
Physical Location & Seq
Forward (+)
17577714 .. 17580397
2684 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G42170.5

Sequence Viewer

Length: 990 bp
ATGGTAGGAATGAATGAGAACGATTTATTTGTGGGAGACGACGCTGAAGCCAGGAGCGGTATACTTACACTTGACTACCCAATGGAGCACGGTGTTGTTAGCAATTGGGATGACATGGAGAAGATTTGGTACCATACGTTTTACAGTGAGCTACGTGTGGCTCCTGAAGAACACCCTGTGCTTCTCACTGAAGCTCCTCTTAACCCGAAAGCCGATCGTGAGAAGATGACTCAGATCATGTTTGAGACATTTGCTGTTCCTAGTATGTATATTGGTATGCAGGCCGCTCTCTCCCTCCATGCCAGTGGTCGTACCACAGGTACTGTGTTGGATTCAGGAGATGGTGTGAGCTACATAGTTCCAATCTACGAAGGTTCAGCACTCCCACATGCAATCTTGCGGCTAGATTTAGCAGGCCGTCACCTAACCAACTACCTCATGAAAATCATGATGGAACGTGGCTACACATCCGCCGAGCGTGAGGTAGTCCGAGACATCAAAGAACAATTTGGTTACATAGCTCTTGACTACGAGCAAGAAATGGAGAAGGCTACAAAGAGTTCGGCAATCGATAGAACCTACGAGCTTCCAGATGGTCAAGTGATCACGATAGGAGCTGAGAGATTCAGGTGCCCTGAGGTTCTTTTCCAGCCGTCTTTGATTGGAATGGAAACTTCTGGTATTCATGAGAAGACTTACAATTCAATCATGAAGTGTGATGATGATATAAGGAAGGATTTGTATGGAAACATTGTGTTGAGTGGTGGGACGACGATGTTCCGAGGGATTGAAGAGAGGATGACCAAAGAGATCAATGCGCTTGCAGCCGCTAATATGAGGATTAAAATTGTTGCTCCCCCGGAAAGGAAATATAGTGTTTGGATTGGAGGCTCCATTTTGGCTTCTCTAAGCACATATGAACAGATGTGGATTACTAAAGCTGAGTATGAAGAAAATGGACCGGCCATTGTTCACACGAAATGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

329

Amino Acids

37.08

Weight (kDa)

4.94

Isoelectric Point (pI)

28.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 9 - 231 3.1e-74 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 129
AccB1I GGYRCC 2 cut(s) 129, 630
AccBSI CCGCTC 2 cut(s) 57, 287
AccI GTMKAC 1 cut(s) 61
AciI CCGC 5 cut(s) 57, 285, 400, 471, 828
AcoI YGGCCR 1 cut(s) 963
AcuI CTGAAG 3 cut(s) 66, 186, 210
AdeI CACNNNGTG 1 cut(s) 178
AfaI GTAC 3 cut(s) 131, 313, 322
AfiI CCNNNNNNNGG 1 cut(s) 864
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 2 cut(s) 705, 791
AjnI CCWGG 1 cut(s) 50
AluBI AGCT 7 cut(s) 151, 194, 351, 521, 586, 617, 941
AluI AGCT 7 cut(s) 151, 194, 351, 521, 586, 617, 941
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 3 cut(s) 30, 239, 486
AlwNI CAGNNNCTG 1 cut(s) 323
AoxI GGCC 3 cut(s) 282, 415, 963
ApeKI GCWGC 1 cut(s) 824
Asp718I GGTACC 1 cut(s) 129
AspLEI GCGC 1 cut(s) 820
AspS9I GGNCC 1 cut(s) 959
AsuC2I CCSGG 1 cut(s) 860
AsuHPI GGTGA 1 cut(s) 413
AvaII GGWCC 1 cut(s) 959
AxyI CCTNAGG 1 cut(s) 636
BaeGI GKGCMC 1 cut(s) 635
BanI GGYRCC 2 cut(s) 129, 630
BarI GAAGNNNNNNTAC 2 cut(s) 113, 145
BbsI GAAGAC 1 cut(s) 698
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 836
BccI CCATC 3 cut(s) 335, 445, 587
BceAI ACGGC 2 cut(s) 402, 637
BciT130I CCWGG 1 cut(s) 52
BclI TGATCA 1 cut(s) 603
BcnI CCSGG 1 cut(s) 860
BcoDI GTCTC 3 cut(s) 30, 239, 486
BfaI CTAG 2 cut(s) 261, 404
BisI GCNGC 4 cut(s) 285, 401, 825, 828
BlsI GCNGC 4 cut(s) 286, 402, 826, 829
Bme1390I CCNGG 2 cut(s) 52, 860
Bme18I GGWCC 1 cut(s) 959
BmgT120I GGNCC 1 cut(s) 959
BmiI GGNNCC 4 cut(s) 131, 162, 632, 892
BmrFI CCNGG 2 cut(s) 52, 860
BpiI GAAGAC 1 cut(s) 698
BpuMI CCSGG 1 cut(s) 860
Bsa29I ATCGAT 1 cut(s) 570
BsaAI YACGTR 1 cut(s) 155
BsaJI CCNNGG 2 cut(s) 781, 858
BsaXI ACNNNNNCTCC 4 cut(s) 178, 208, 330, 360
Bsc4I CCNNNNNNNGG 1 cut(s) 864
Bse118I RCCGGY 1 cut(s) 961
Bse1I ACTGG 1 cut(s) 303
Bse21I CCTNAGG 1 cut(s) 636
BseBI CCWGG 1 cut(s) 52
BseCI ATCGAT 1 cut(s) 570
BseDI CCNNGG 2 cut(s) 781, 858
BseGI GGATG 3 cut(s) 115, 467, 804
BseLI CCNNNNNNNGG 1 cut(s) 864
BseMII CTCAG 4 cut(s) 245, 609, 627, 933
BseNI ACTGG 1 cut(s) 303
BseRI GAGGAG 1 cut(s) 186
BseSI GKGCMC 1 cut(s) 635
BseXI GCAGC 1 cut(s) 836
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 3 cut(s) 284, 417, 965
BshNI GGYRCC 2 cut(s) 129, 630
BshVI ATCGAT 1 cut(s) 570
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 2 cut(s) 860, 962
BslFI GGGAC 1 cut(s) 781
BslI CCNNNNNNNGG 1 cut(s) 864
BsmAI GTCTC 3 cut(s) 30, 239, 486
BsmBI CGTCTC 1 cut(s) 30
BsmFI GGGAC 1 cut(s) 781
BsnI GGCC 3 cut(s) 284, 417, 965
Bsp1286I GDGCHC 2 cut(s) 90, 635
Bsp143I GATC 4 cut(s) 214, 234, 603, 810
BspACI CCGC 5 cut(s) 57, 285, 400, 471, 828
BspANI GGCC 3 cut(s) 284, 417, 965
BspCNI CTCAG 4 cut(s) 244, 610, 628, 934
BspDI ATCGAT 1 cut(s) 570
BspHI TCATGA 4 cut(s) 438, 447, 685, 708
BspLI GGNNCC 4 cut(s) 131, 162, 632, 892
BspT107I GGYRCC 2 cut(s) 129, 630
BsrBI CCGCTC 2 cut(s) 57, 287
BsrFI RCCGGY 1 cut(s) 961
BsrI ACTGG 1 cut(s) 303
BssAI RCCGGY 1 cut(s) 961
BssECI CCNNGG 2 cut(s) 781, 858
BssMI GATC 4 cut(s) 214, 234, 603, 810
BssNAI GTATAC 1 cut(s) 62
Bst1107I GTATAC 1 cut(s) 62
Bst2UI CCWGG 1 cut(s) 52
Bst4CI ACNGT 3 cut(s) 92, 146, 325
Bst6I CTCTTC 1 cut(s) 786
BstBAI YACGTR 1 cut(s) 155
BstC8I GCNNGC 3 cut(s) 282, 415, 822
BstDEI CTNAG 5 cut(s) 231, 618, 636, 908, 942
BstF5I GGATG 3 cut(s) 115, 467, 804
BstHHI GCGC 1 cut(s) 820
BstKTI GATC 4 cut(s) 217, 237, 606, 813
BstMAI GTCTC 3 cut(s) 30, 239, 486
BstMBI GATC 4 cut(s) 214, 234, 603, 810
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 1 cut(s) 824
BstNI CCWGG 1 cut(s) 52
BstNSI RCATGY 1 cut(s) 392
BstSCI CCNGG 2 cut(s) 50, 858
BstSLI GKGCMC 1 cut(s) 635
BstV1I GCAGC 1 cut(s) 836
BstV2I GAAGAC 1 cut(s) 698
BstXI CCANNNNNNTGG 1 cut(s) 305
BstZ17I GTATAC 1 cut(s) 62
Bsu15I ATCGAT 1 cut(s) 570
Bsu36I CCTNAGG 1 cut(s) 636
BsuRI GGCC 3 cut(s) 284, 417, 965
BsuTUI ATCGAT 1 cut(s) 570
BtsCI GGATG 3 cut(s) 115, 467, 804
BtsIMutI CAGTG 3 cut(s) 151, 186, 310
Cac8I GCNNGC 3 cut(s) 282, 415, 822
CaiI CAGNNNCTG 1 cut(s) 323
CciI TCATGA 4 cut(s) 438, 447, 685, 708
CfoI GCGC 1 cut(s) 820
Cfr10I RCCGGY 1 cut(s) 961
Cfr13I GGNCC 1 cut(s) 959
ClaI ATCGAT 1 cut(s) 570
CseI GACGC 1 cut(s) 50
Csp6I GTAC 3 cut(s) 130, 312, 321
CviAII CATG 8 cut(s) 115, 238, 299, 389, 439, 448, 686, 709
CviQI GTAC 3 cut(s) 130, 312, 321
DdeI CTNAG 5 cut(s) 231, 618, 636, 908, 942
DpnI GATC 4 cut(s) 216, 236, 605, 812
DpnII GATC 4 cut(s) 214, 234, 603, 810
DraIII CACNNNGTG 1 cut(s) 178
EaeI YGGCCR 1 cut(s) 963
Eam1104I CTCTTC 1 cut(s) 786
EarI CTCTTC 1 cut(s) 786
EciI GGCGGA 1 cut(s) 460
Eco47I GGWCC 1 cut(s) 959
Eco57I CTGAAG 3 cut(s) 66, 186, 210
Eco81I CCTNAGG 1 cut(s) 636
EcoRII CCWGG 1 cut(s) 50
Esp3I CGTCTC 1 cut(s) 30
FaeI CATG 8 cut(s) 118, 241, 302, 392, 442, 451, 689, 712
FalI AAGNNNNNCTT 2 cut(s) 183, 215
FaqI GGGAC 1 cut(s) 781
FatI CATG 8 cut(s) 114, 237, 298, 388, 438, 447, 685, 708
FauNDI CATATG 1 cut(s) 916
FbaI TGATCA 1 cut(s) 603
FblI GTMKAC 1 cut(s) 61
Fnu4HI GCNGC 4 cut(s) 285, 401, 825, 828
FokI GGATG 3 cut(s) 122, 454, 811
Fsp4HI GCNGC 4 cut(s) 285, 401, 825, 828
FspBI CTAG 2 cut(s) 261, 404
GlaI GCGC 1 cut(s) 819
GluI GCNGC 4 cut(s) 285, 401, 825, 828
HaeIII GGCC 3 cut(s) 284, 417, 965
HapII CCGG 2 cut(s) 860, 962
HgaI GACGC 1 cut(s) 50
HhaI GCGC 1 cut(s) 820
Hin1II CATG 8 cut(s) 118, 241, 302, 392, 442, 451, 689, 712
Hin6I GCGC 1 cut(s) 818
HinP1I GCGC 1 cut(s) 818
HinfI GANTC 3 cut(s) 229, 332, 624
HpaII CCGG 2 cut(s) 860, 962
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 2 cut(s) 62, 973
Hpy188I TCNGA 3 cut(s) 234, 491, 782
Hpy8I GTNNAC 2 cut(s) 62, 973
Hpy99I CGWCG 2 cut(s) 44, 775
HpyAV CCTTC 3 cut(s) 365, 541, 727
HpyCH4III ACNGT 3 cut(s) 92, 146, 325
HpyCH4IV ACGT 3 cut(s) 137, 154, 457
HpyCH4V TGCA 3 cut(s) 280, 392, 824
HpyF10VI GCNNNNNNNGC 1 cut(s) 824
HpyF3I CTNAG 5 cut(s) 231, 618, 636, 908, 942
HpySE526I ACGT 3 cut(s) 137, 154, 457
Hsp92II CATG 8 cut(s) 118, 241, 302, 392, 442, 451, 689, 712
HspAI GCGC 1 cut(s) 818
KpnI GGTACC 1 cut(s) 133
Ksp22I TGATCA 1 cut(s) 603
Kzo9I GATC 4 cut(s) 214, 234, 603, 810
LmnI GCTCC 7 cut(s) 54, 85, 166, 199, 614, 859, 896
Lsp1109I GCAGC 1 cut(s) 836
MaeI CTAG 2 cut(s) 261, 404
MaeII ACGT 3 cut(s) 137, 154, 457
MaeIII GTNAC 2 cut(s) 419, 512
MalI GATC 4 cut(s) 216, 236, 605, 812
MbiI CCGCTC 2 cut(s) 57, 287
MboI GATC 4 cut(s) 214, 234, 603, 810
MboII GAAGA 6 cut(s) 133, 179, 235, 703, 803, 962
MfeI CAATTG 1 cut(s) 103
MhlI GDGCHC 2 cut(s) 90, 635
MluCI AATT 4 cut(s) 103, 506, 700, 846
MlyI GAGTC 1 cut(s) 223
MmeI TCCRAC 1 cut(s) 309
MnlI CCTC 9 cut(s) 207, 305, 446, 475, 631, 776, 789, 831, 881
MseI TTAA 2 cut(s) 201, 843
MslI CAYNNNNRTG 1 cut(s) 303
MspI CCGG 2 cut(s) 860, 962
MspR9I CCNGG 2 cut(s) 52, 860
MunI CAATTG 1 cut(s) 103
MvaI CCWGG 1 cut(s) 52
MwoI GCNNNNNNNGC 1 cut(s) 824
NciI CCSGG 1 cut(s) 860
NdeI CATATG 1 cut(s) 916
NdeII GATC 4 cut(s) 214, 234, 603, 810
NlaIII CATG 8 cut(s) 118, 241, 302, 392, 442, 451, 689, 712
NlaIV GGNNCC 4 cut(s) 131, 162, 632, 892
NmeAIII GCCGAG 1 cut(s) 499
NmuCI GTSAC 1 cut(s) 419
NspI RCATGY 1 cut(s) 392
PagI TCATGA 4 cut(s) 438, 447, 685, 708
PfeI GAWTC 2 cut(s) 332, 624
PkrI GCNGC 4 cut(s) 286, 402, 826, 829
Ple19I CGATCG 1 cut(s) 217
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
Ppu21I YACGTR 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 50
PspGI CCWGG 1 cut(s) 50
PspN4I GGNNCC 4 cut(s) 131, 162, 632, 892
PspPI GGNCC 1 cut(s) 959
PstNI CAGNNNCTG 1 cut(s) 323
PvuI CGATCG 1 cut(s) 217
RsaI GTAC 3 cut(s) 131, 313, 322
RsaNI GTAC 3 cut(s) 130, 312, 321
RseI CAYNNNNRTG 1 cut(s) 303
SaqAI TTAA 2 cut(s) 201, 843
SatI GCNGC 4 cut(s) 285, 401, 825, 828
Sau3AI GATC 4 cut(s) 214, 234, 603, 810
Sau96I GGNCC 1 cut(s) 959
SchI GAGTC 1 cut(s) 223
ScrFI CCNGG 2 cut(s) 52, 860
SduI GDGCHC 2 cut(s) 90, 635
SinI GGWCC 1 cut(s) 959
SmiMI CAYNNNNRTG 1 cut(s) 303
Sse9I AATT 4 cut(s) 103, 506, 700, 846
SsiI CCGC 5 cut(s) 57, 285, 400, 471, 828
SspMI CTAG 2 cut(s) 261, 404
StyD4I CCNGG 2 cut(s) 50, 858
TaaI ACNGT 3 cut(s) 92, 146, 325
TaiI ACGT 3 cut(s) 140, 157, 460
TaqI TCGA 1 cut(s) 570
TasI AATT 4 cut(s) 103, 506, 700, 846
TauI GCSGC 3 cut(s) 287, 403, 830
TfiI GAWTC 2 cut(s) 332, 624
Tru1I TTAA 2 cut(s) 201, 843
Tru9I TTAA 2 cut(s) 201, 843
TscAI CASTG 3 cut(s) 151, 193, 310
TseFI GTSAC 1 cut(s) 419
TseI GCWGC 1 cut(s) 824
Tsp45I GTSAC 1 cut(s) 419
TspDTI ATGAA 6 cut(s) 26, 455, 674, 725, 933, 963
TspRI CASTG 3 cut(s) 151, 193, 310
VpaK11BI GGWCC 1 cut(s) 959
XceI RCATGY 1 cut(s) 392
XmiI GTMKAC 1 cut(s) 61
XspI CTAG 2 cut(s) 261, 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.