MD06G1127300.v1.1

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008204
Physical Location & Seq
Forward (+)
26929541 .. 26933195
3655 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1127300.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGGTTAAGGCTGGATTTGCCGGAGATGATGCTCCAAGGGCTGTTTTCCCCAGTATTGTTGGTCGTCCTAGACACACAGGTGTTATGGTGGGGATGGGACAGAAAGATGCCTATGTCGGGGATGAGGCCCAGTCGAAAAGAGGTATTCTCACATTAAAATACCCTATTGAACACGGGATTGTTAGCAACTGGGATGACATGGAAAAGATCTGGCATCATACTTTCTACAATGAGCTTCGTGTCTCTCCTGAGGAACATCCTGTGCTCCTTACTGAAGCACCCCTGAATCCTAAGGCCAACAGGGAAAAGATGACTCAGATCATGTTTGAGACCTTCAATGTCCCTGCTATGTATGTTGCCATCCAAGCAGTTCTTTCCCTCTATGCTAGTGGGCGTACTACAGGTATTGTGCTGGACTCTGGTGATGGAGTCAGTCACACAGTTCCCATCTACGAGGGTTATGCACTTCCACATGCCATCCTCCGGCTTGACCTTGCTGGACGAGATTTAACAGATTCCTTGATGAAGATCCTGACTGAGAGGGGGTATACTTTTACTACAACTGCTGAACGGGAAATTGTACGCGACATCAAGGAGAAGCTTGCCTATGTGGCACTTGATTATGAACAGGAACTTGAAACTGCTAAAAGCAGCTCATCAATTGAGAAGAGCTACGAACTACCTGATGGTCAGGTGATTACCATTGGAGCAGAGCGTTTCCGTTGCCCTGAGGTCCTATTTCAACCATCATTCGTTGGAATGGAATCGCCTGGAATTCACGAGACCACCTATAACTCTATTATGAAGTGCGATGTTGACATTAGAAAAGACTTGTATGGCAACATTGTCCTCAGCGGAGGGTCCACTATGTTCCCAGGAATTGCTGATCGAATGAGCAAGGAGATCACAGCCCTCGCACCAAGCAGCGTGAAAATTAAGGTGGTAGCACCTCCAGAGAGAAAGTATAGTGTTTGGATTGGAGGATCCATATTAGCTTCACTGAGCACATTCCAGCAGATGTGGATTTCTAAGTCTGAGTATGACGAATCAGGCCCGTCCATCGTCCACAGGAAGTGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

361

Amino Acids

40.0

Weight (kDa)

5.67

Isoelectric Point (pI)

40.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 1 - 360 1.9e-142 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 548
AccII CGCG 1 cut(s) 585
AciI CCGC 1 cut(s) 855
AclWI GGATC 3 cut(s) 523, 978, 991
AcsI RAATTY 1 cut(s) 774
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 2 cut(s) 397, 582
AfiI CCNNNNNNNGG 2 cut(s) 117, 483
AgsI TTSAA 4 cut(s) 170, 337, 638, 743
AjnI CCWGG 2 cut(s) 769, 874
AleI CACNNNNGTG 1 cut(s) 78
AluBI AGCT 5 cut(s) 235, 601, 654, 672, 995
AluI AGCT 5 cut(s) 235, 601, 654, 672, 995
Alw21I GWGCWC 2 cut(s) 267, 1007
Alw26I GTCTC 3 cut(s) 247, 323, 776
AlwI GGATC 3 cut(s) 523, 978, 991
AoxI GGCC 3 cut(s) 126, 294, 1051
ApeKI GCWGC 2 cut(s) 651, 924
ApoI RAATTY 1 cut(s) 774
Asp700I GAANNNNTTC 1 cut(s) 1076
AspS9I GGNCC 4 cut(s) 127, 733, 861, 1052
AsuHPI GGTGA 2 cut(s) 434, 706
AvaII GGWCC 2 cut(s) 733, 861
AxyI CCTNAGG 3 cut(s) 249, 291, 729
BamHI GGATCC 1 cut(s) 983
BauI CACGAG 1 cut(s) 779
Bbv12I GWGCWC 2 cut(s) 267, 1007
BbvCI CCTCAGC 1 cut(s) 851
BbvI GCAGC 2 cut(s) 663, 936
BccI CCATC 8 cut(s) 88, 368, 419, 455, 485, 680, 754, 1067
BcgI CGANNNNNNTGC 2 cut(s) 443, 477
BciT130I CCWGG 2 cut(s) 771, 876
BcoDI GTCTC 3 cut(s) 247, 323, 776
BfaI CTAG 3 cut(s) 69, 387, 1081
BfmI CTRYAG 1 cut(s) 399
BglI GCCNNNNNGGC 1 cut(s) 611
BglII AGATCT 1 cut(s) 207
BisI GCNGC 2 cut(s) 652, 925
BlsI GCNGC 2 cut(s) 653, 926
Bme1390I CCNGG 2 cut(s) 771, 876
Bme18I GGWCC 2 cut(s) 733, 861
BmgT120I GGNCC 4 cut(s) 127, 733, 861, 1052
BmiI GGNNCC 2 cut(s) 862, 985
BmrFI CCNGG 2 cut(s) 771, 876
BmrI ACTGGG 3 cut(s) 45, 124, 199
BmsI GCATC 3 cut(s) 19, 97, 223
BmuI ACTGGG 3 cut(s) 45, 124, 199
BplI GAGNNNNNCTC 2 cut(s) 132, 164
BpmI CTGGAG 1 cut(s) 936
Bpu10I CCTNAGC 1 cut(s) 851
BsaBI GATNNNNATC 1 cut(s) 527
BsaI GGTCTC 2 cut(s) 323, 776
BsaJI CCNNGG 2 cut(s) 35, 874
Bsc4I CCNNNNNNNGG 2 cut(s) 117, 483
Bse1I ACTGG 3 cut(s) 51, 130, 194
Bse21I CCTNAGG 3 cut(s) 249, 291, 729
Bse8I GATNNNNATC 1 cut(s) 527
BseBI CCWGG 2 cut(s) 771, 876
BseDI CCNNGG 2 cut(s) 35, 874
BseGI GGATG 6 cut(s) 99, 127, 199, 256, 360, 477
BseJI GATNNNNATC 1 cut(s) 527
BseLI CCNNNNNNNGG 2 cut(s) 117, 483
BseMII CTCAG 7 cut(s) 240, 329, 528, 720, 865, 992, 1026
BseNI ACTGG 3 cut(s) 51, 130, 194
BseXI GCAGC 2 cut(s) 663, 936
Bsh1236I CGCG 1 cut(s) 585
BshFI GGCC 3 cut(s) 128, 296, 1053
BsiHKAI GWGCWC 2 cut(s) 267, 1007
BsiSI CCGG 2 cut(s) 21, 484
BslFI GGGAC 2 cut(s) 111, 326
BslI CCNNNNNNNGG 2 cut(s) 117, 483
BsmAI GTCTC 3 cut(s) 247, 323, 776
BsmFI GGGAC 2 cut(s) 111, 326
BsnI GGCC 3 cut(s) 128, 296, 1053
Bso31I GGTCTC 2 cut(s) 323, 776
Bsp1286I GDGCHC 2 cut(s) 267, 1007
Bsp143I GATC 6 cut(s) 207, 318, 528, 886, 903, 983
BspACI CCGC 1 cut(s) 855
BspANI GGCC 3 cut(s) 128, 296, 1053
BspCNI CTCAG 7 cut(s) 241, 328, 529, 721, 864, 993, 1027
BspFNI CGCG 1 cut(s) 585
BspLI GGNNCC 2 cut(s) 862, 985
BspPI GGATC 3 cut(s) 523, 978, 991
BspQI GCTCTTC 1 cut(s) 662
BspTNI GGTCTC 2 cut(s) 323, 776
BsrI ACTGG 3 cut(s) 51, 130, 194
BssECI CCNNGG 2 cut(s) 35, 874
BssMI GATC 6 cut(s) 207, 318, 528, 886, 903, 983
BssNAI GTATAC 1 cut(s) 549
BssSI CACGAG 1 cut(s) 779
BssT1I CCWWGG 1 cut(s) 35
Bst1107I GTATAC 1 cut(s) 549
Bst2BI CACGAG 1 cut(s) 779
Bst2UI CCWGG 2 cut(s) 771, 876
Bst4CI ACNGT 1 cut(s) 442
Bst6I CTCTTC 1 cut(s) 662
BstC8I GCNNGC 1 cut(s) 603
BstDEI CTNAG 9 cut(s) 249, 291, 315, 537, 729, 851, 1001, 1029, 1035
BstF5I GGATG 6 cut(s) 99, 127, 199, 256, 360, 477
BstFNI CGCG 1 cut(s) 585
BstKTI GATC 6 cut(s) 210, 321, 531, 889, 906, 986
BstMAI GTCTC 3 cut(s) 247, 323, 776
BstMBI GATC 6 cut(s) 207, 318, 528, 886, 903, 983
BstMWI GCNNNNNNNGC 4 cut(s) 17, 38, 365, 611
BstNI CCWGG 2 cut(s) 771, 876
BstNSI RCATGY 1 cut(s) 476
BstSCI CCNGG 2 cut(s) 769, 874
BstSFI CTRYAG 1 cut(s) 399
BstUI CGCG 1 cut(s) 585
BstV1I GCAGC 2 cut(s) 663, 936
BstX2I RGATCY 3 cut(s) 207, 528, 983
BstYI RGATCY 3 cut(s) 207, 528, 983
BstZ17I GTATAC 1 cut(s) 549
Bsu36I CCTNAGG 3 cut(s) 249, 291, 729
BsuRI GGCC 3 cut(s) 128, 296, 1053
BtgZI GCGATG 1 cut(s) 825
BtsCI GGATG 6 cut(s) 99, 127, 199, 256, 360, 477
BtsIMutI CAGTG 1 cut(s) 998
Cac8I GCNNGC 1 cut(s) 603
Cfr13I GGNCC 4 cut(s) 127, 733, 861, 1052
Csp6I GTAC 2 cut(s) 396, 581
CviAII CATG 3 cut(s) 199, 322, 473
CviQI GTAC 2 cut(s) 396, 581
DdeI CTNAG 9 cut(s) 249, 291, 315, 537, 729, 851, 1001, 1029, 1035
DpnI GATC 6 cut(s) 209, 320, 530, 888, 905, 985
DpnII GATC 6 cut(s) 207, 318, 528, 886, 903, 983
Eam1104I CTCTTC 1 cut(s) 662
EarI CTCTTC 1 cut(s) 662
Eco130I CCWWGG 1 cut(s) 35
Eco31I GGTCTC 2 cut(s) 323, 776
Eco47I GGWCC 2 cut(s) 733, 861
Eco57I CTGAAG 1 cut(s) 294
Eco81I CCTNAGG 3 cut(s) 249, 291, 729
EcoO109I RGGNCCY 1 cut(s) 733
EcoRI GAATTC 1 cut(s) 774
EcoRII CCWGG 2 cut(s) 769, 874
EcoT14I CCWWGG 1 cut(s) 35
ErhI CCWWGG 1 cut(s) 35
FaeI CATG 3 cut(s) 202, 325, 476
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 2 cut(s) 111, 326
FatI CATG 3 cut(s) 198, 321, 472
FblI GTMKAC 1 cut(s) 548
Fnu4HI GCNGC 2 cut(s) 652, 925
FokI GGATG 6 cut(s) 106, 134, 206, 243, 347, 464
Fsp4HI GCNGC 2 cut(s) 652, 925
FspBI CTAG 3 cut(s) 69, 387, 1081
GluI GCNGC 2 cut(s) 652, 925
GsuI CTGGAG 1 cut(s) 936
HaeIII GGCC 3 cut(s) 128, 296, 1053
HapII CCGG 2 cut(s) 21, 484
Hin1II CATG 3 cut(s) 202, 325, 476
HincII GTYRAC 1 cut(s) 817
HindII GTYRAC 1 cut(s) 817
HindIII AAGCTT 1 cut(s) 599
HinfI GANTC 7 cut(s) 286, 313, 416, 429, 515, 764, 1046
HpaII CCGG 2 cut(s) 21, 484
HphI GGTGA 2 cut(s) 434, 706
Hpy166II GTNNAC 4 cut(s) 549, 817, 864, 1066
Hpy188I TCNGA 2 cut(s) 318, 1036
Hpy188III TCNNGA 4 cut(s) 248, 532, 779, 953
Hpy8I GTNNAC 4 cut(s) 549, 817, 864, 1066
HpyAV CCTTC 1 cut(s) 343
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4V TGCA 1 cut(s) 464
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 38, 365, 611
HpyF3I CTNAG 9 cut(s) 249, 291, 315, 537, 729, 851, 1001, 1029, 1035
Hsp92II CATG 3 cut(s) 202, 325, 476
Kzo9I GATC 6 cut(s) 207, 318, 528, 886, 903, 983
LguI GCTCTTC 1 cut(s) 662
LmnI GCTCC 3 cut(s) 37, 270, 707
Lsp1109I GCAGC 2 cut(s) 663, 936
LweI GCATC 3 cut(s) 19, 97, 223
MaeI CTAG 3 cut(s) 69, 387, 1081
MaeIII GTNAC 1 cut(s) 434
MalI GATC 6 cut(s) 209, 320, 530, 888, 905, 985
MboI GATC 6 cut(s) 207, 318, 528, 886, 903, 983
MboII GAAGA 2 cut(s) 538, 679
MfeI CAATTG 1 cut(s) 660
MflI RGATCY 3 cut(s) 207, 528, 983
MhlI GDGCHC 2 cut(s) 267, 1007
MluCI AATT 5 cut(s) 576, 660, 774, 879, 933
MlyI GAGTC 3 cut(s) 307, 410, 438
MmeI TCCRAC 1 cut(s) 736
MroXI GAANNNNTTC 1 cut(s) 1076
MseI TTAA 4 cut(s) 6, 155, 509, 936
MslI CAYNNNNRTG 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 855
MspI CCGG 2 cut(s) 21, 484
MspR9I CCNGG 2 cut(s) 771, 876
MunI CAATTG 1 cut(s) 660
MvaI CCWGG 2 cut(s) 771, 876
MvnI CGCG 1 cut(s) 585
MwoI GCNNNNNNNGC 4 cut(s) 17, 38, 365, 611
NdeII GATC 6 cut(s) 207, 318, 528, 886, 903, 983
NlaIII CATG 3 cut(s) 202, 325, 476
NlaIV GGNNCC 2 cut(s) 862, 985
NmuCI GTSAC 1 cut(s) 434
NspI RCATGY 1 cut(s) 476
OliI CACNNNNGTG 1 cut(s) 78
PciSI GCTCTTC 1 cut(s) 662
PdmI GAANNNNTTC 1 cut(s) 1076
PfeI GAWTC 4 cut(s) 286, 515, 764, 1046
PkrI GCNGC 2 cut(s) 653, 926
PleI GAGTC 3 cut(s) 307, 410, 437
PpsI GAGTC 3 cut(s) 307, 410, 437
PpuMI RGGWCCY 1 cut(s) 733
Psp5II RGGWCCY 1 cut(s) 733
Psp6I CCWGG 2 cut(s) 769, 874
PspGI CCWGG 2 cut(s) 769, 874
PspN4I GGNNCC 2 cut(s) 862, 985
PspPI GGNCC 4 cut(s) 127, 733, 861, 1052
PspPPI RGGWCCY 1 cut(s) 733
PsuI RGATCY 3 cut(s) 207, 528, 983
RsaI GTAC 2 cut(s) 397, 582
RsaNI GTAC 2 cut(s) 396, 581
RseI CAYNNNNRTG 1 cut(s) 78
SapI GCTCTTC 1 cut(s) 662
SaqAI TTAA 4 cut(s) 6, 155, 509, 936
SatI GCNGC 2 cut(s) 652, 925
Sau3AI GATC 6 cut(s) 207, 318, 528, 886, 903, 983
Sau96I GGNCC 4 cut(s) 127, 733, 861, 1052
SchI GAGTC 3 cut(s) 307, 410, 438
ScrFI CCNGG 2 cut(s) 771, 876
SduI GDGCHC 2 cut(s) 267, 1007
SfaNI GCATC 3 cut(s) 19, 97, 223
SfcI CTRYAG 1 cut(s) 399
SinI GGWCC 2 cut(s) 733, 861
SmiMI CAYNNNNRTG 1 cut(s) 78
Sse9I AATT 5 cut(s) 576, 660, 774, 879, 933
SsiI CCGC 1 cut(s) 855
SspMI CTAG 3 cut(s) 69, 387, 1081
StyD4I CCNGG 2 cut(s) 769, 874
StyI CCWWGG 1 cut(s) 35
TaaI ACNGT 1 cut(s) 442
TaqI TCGA 2 cut(s) 134, 889
TasI AATT 5 cut(s) 576, 660, 774, 879, 933
TfiI GAWTC 4 cut(s) 286, 515, 764, 1046
Tru1I TTAA 4 cut(s) 6, 155, 509, 936
Tru9I TTAA 4 cut(s) 6, 155, 509, 936
TscAI CASTG 1 cut(s) 1005
TseFI GTSAC 1 cut(s) 434
TseI GCWGC 2 cut(s) 651, 924
Tsp45I GTSAC 1 cut(s) 434
TspDTI ATGAA 3 cut(s) 539, 639, 818
TspGWI ACGGA 1 cut(s) 710
TspRI CASTG 1 cut(s) 1005
VpaK11BI GGWCC 2 cut(s) 733, 861
XapI RAATTY 1 cut(s) 774
XceI RCATGY 1 cut(s) 476
XmiI GTMKAC 1 cut(s) 548
XmnI GAANNNNTTC 1 cut(s) 1076
XspI CTAG 3 cut(s) 69, 387, 1081
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.