MD14G1142600.v1.1

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008204
Physical Location & Seq
Forward (+)
23543235 .. 23546647
3413 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1142600.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGGTTAAGGCTGGATTTGCTGGAGATGATGCTCCGAGGGCTGTTTTCCCCAGTATTGTTGGTCGTCCTAGACACACAGGTGTTATGGTGGGGATGGGACAGAAGGATGCCTATGTTGGGGATGAGGCCCAGTCGAAAAGGGGTATTCTCACATTAAAATACCCCATTGAACACGGGATTGTTAGCAATTGGGATGACATGGAAAAGATCTGGCATCATACTTTCTACAATGAGCTTCGTGTCGCTCCTGAAGAACATCCTGTGCTTCTTACTGAAGCACCTCTGAATCCTAAGGCCAACAGGGAAAAGATGACTCAGATCATGTTTGAGACCTTTAATGTCCCTGCTATGTATGTTGCCATTCAAGCAGTTCTCTCCCTCTATGCTAGTGGGCGTACTACAGGTATTGTGCTTGACTCTGGTGATGGAGTCAGTCACACTGTTCCCATCTATGAGGGTTATGCACTTCCACATGCCATCCTCCGGCTTGACCTTGCTGGACGAGACTTAACAGATTCCTTAATGAAGATCCTGACTGAGAGAGGGTATACTTTTACTACAACTGCTGAACGGGAAATTGTGCGTGACATTAAGGAGAAGCTTGCCTATGTGGCACTTGATTATGAACAGGAACTTGAAACTGCTAAAAGCAGCTCATCAATTGAGAAGAGTTATGAGCTACCTGATGGCCAGGTAATTACCATTGGGGCAGAGCGTTTCCGTTGCCCTGAGGTCCTATTTCAACCATCATTCATTGGAATGGAATCGCCTGGGATTCATGAGACCACCTATAACTCTATTATGAAGTGTGATGTTGACATCAGAAAGGACTTGTATGGCAACATCGTCCTCAGTGGAGGGTCCACTATGTTCCCAGGAATTGCTGATCGAATGAGCAAGGAGATCACAGCCCTCGCACCAAGTAGCATGAAAATTAAGGTGGTTGCACCTCCAGAGAGAAAGTATAGTGTTTGGATTGGAGGATCCATATTAGCTTCACTGAGCACCTTCCAGCAGATGTGGATTTCTAAGTCGGAGTACGACGAATCAGGTCCGTCCATCGTCCACAGGAAGTGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

361

Amino Acids

40.03

Weight (kDa)

5.67

Isoelectric Point (pI)

40.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 1 - 360 1.4e-142 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 548
AclWI GGATC 3 cut(s) 523, 978, 991
AcoI YGGCCR 1 cut(s) 688
AcuI CTGAAG 2 cut(s) 270, 294
AfaI GTAC 2 cut(s) 397, 1040
AfiI CCNNNNNNNGG 2 cut(s) 117, 483
AgsI TTSAA 4 cut(s) 170, 365, 638, 743
AjnI CCWGG 3 cut(s) 690, 769, 874
AleI CACNNNNGTG 1 cut(s) 78
AluBI AGCT 5 cut(s) 235, 601, 654, 679, 995
AluI AGCT 5 cut(s) 235, 601, 654, 679, 995
Alw21I GWGCWC 1 cut(s) 1007
Alw26I GTCTC 3 cut(s) 323, 498, 776
AlwI GGATC 3 cut(s) 523, 978, 991
AoxI GGCC 3 cut(s) 126, 294, 688
ApeKI GCWGC 1 cut(s) 651
Asp700I GAANNNNTTC 1 cut(s) 1076
AspS9I GGNCC 4 cut(s) 127, 733, 861, 1052
AsuHPI GGTGA 1 cut(s) 434
AvaII GGWCC 3 cut(s) 733, 861, 1052
AxyI CCTNAGG 2 cut(s) 291, 729
BalI TGGCCA 1 cut(s) 690
BamHI GGATCC 1 cut(s) 983
Bbv12I GWGCWC 1 cut(s) 1007
BbvI GCAGC 1 cut(s) 663
BccI CCATC 7 cut(s) 88, 419, 455, 485, 680, 754, 1067
BciT130I CCWGG 3 cut(s) 692, 771, 876
BcoDI GTCTC 3 cut(s) 323, 498, 776
BfaI CTAG 3 cut(s) 69, 387, 1081
BfmI CTRYAG 1 cut(s) 399
BglI GCCNNNNNGGC 1 cut(s) 611
BglII AGATCT 1 cut(s) 207
BisI GCNGC 1 cut(s) 652
BlsI GCNGC 1 cut(s) 653
Bme1390I CCNGG 3 cut(s) 692, 771, 876
Bme18I GGWCC 3 cut(s) 733, 861, 1052
BmgT120I GGNCC 4 cut(s) 127, 733, 861, 1052
BmiI GGNNCC 2 cut(s) 862, 985
BmrFI CCNGG 3 cut(s) 692, 771, 876
BmrI ACTGGG 2 cut(s) 45, 124
BmsI GCATC 3 cut(s) 19, 97, 223
BmuI ACTGGG 2 cut(s) 45, 124
BpmI CTGGAG 2 cut(s) 42, 936
BsaI GGTCTC 2 cut(s) 323, 776
BsaJI CCNNGG 3 cut(s) 35, 770, 874
Bsc4I CCNNNNNNNGG 2 cut(s) 117, 483
Bse1I ACTGG 2 cut(s) 51, 130
Bse21I CCTNAGG 2 cut(s) 291, 729
BseBI CCWGG 3 cut(s) 692, 771, 876
BseDI CCNNGG 3 cut(s) 35, 770, 874
BseGI GGATG 6 cut(s) 99, 112, 127, 199, 256, 477
BseLI CCNNNNNNNGG 2 cut(s) 117, 483
BseMII CTCAG 5 cut(s) 329, 528, 720, 865, 992
BseNI ACTGG 2 cut(s) 51, 130
BseXI GCAGC 1 cut(s) 663
BshFI GGCC 3 cut(s) 128, 296, 690
BsiHKAI GWGCWC 1 cut(s) 1007
BsiSI CCGG 1 cut(s) 484
BslFI GGGAC 2 cut(s) 111, 326
BslI CCNNNNNNNGG 2 cut(s) 117, 483
BsmAI GTCTC 3 cut(s) 323, 498, 776
BsmFI GGGAC 2 cut(s) 111, 326
BsnI GGCC 3 cut(s) 128, 296, 690
Bso31I GGTCTC 2 cut(s) 323, 776
Bsp1286I GDGCHC 1 cut(s) 1007
Bsp143I GATC 6 cut(s) 207, 318, 528, 886, 903, 983
BspANI GGCC 3 cut(s) 128, 296, 690
BspCNI CTCAG 5 cut(s) 328, 529, 721, 864, 993
BspHI TCATGA 1 cut(s) 778
BspLI GGNNCC 2 cut(s) 862, 985
BspPI GGATC 3 cut(s) 523, 978, 991
BspTNI GGTCTC 2 cut(s) 323, 776
BsrI ACTGG 2 cut(s) 51, 130
BssECI CCNNGG 3 cut(s) 35, 770, 874
BssMI GATC 6 cut(s) 207, 318, 528, 886, 903, 983
BssNAI GTATAC 1 cut(s) 549
Bst1107I GTATAC 1 cut(s) 549
Bst2UI CCWGG 3 cut(s) 692, 771, 876
Bst4CI ACNGT 1 cut(s) 442
Bst6I CTCTTC 1 cut(s) 662
BstC8I GCNNGC 1 cut(s) 603
BstDEI CTNAG 7 cut(s) 291, 315, 537, 729, 851, 1001, 1029
BstF5I GGATG 6 cut(s) 99, 112, 127, 199, 256, 477
BstKTI GATC 6 cut(s) 210, 321, 531, 889, 906, 986
BstMAI GTCTC 3 cut(s) 323, 498, 776
BstMBI GATC 6 cut(s) 207, 318, 528, 886, 903, 983
BstMWI GCNNNNNNNGC 4 cut(s) 17, 38, 365, 611
BstNI CCWGG 3 cut(s) 692, 771, 876
BstNSI RCATGY 1 cut(s) 476
BstSCI CCNGG 3 cut(s) 690, 769, 874
BstSFI CTRYAG 1 cut(s) 399
BstV1I GCAGC 1 cut(s) 663
BstX2I RGATCY 3 cut(s) 207, 528, 983
BstYI RGATCY 3 cut(s) 207, 528, 983
BstZ17I GTATAC 1 cut(s) 549
Bsu36I CCTNAGG 2 cut(s) 291, 729
BsuRI GGCC 3 cut(s) 128, 296, 690
BtsCI GGATG 6 cut(s) 99, 112, 127, 199, 256, 477
BtsIMutI CAGTG 3 cut(s) 438, 859, 998
Cac8I GCNNGC 1 cut(s) 603
CciI TCATGA 1 cut(s) 778
Cfr13I GGNCC 4 cut(s) 127, 733, 861, 1052
Csp6I GTAC 2 cut(s) 396, 1039
CviAII CATG 5 cut(s) 199, 322, 473, 779, 928
CviQI GTAC 2 cut(s) 396, 1039
DdeI CTNAG 7 cut(s) 291, 315, 537, 729, 851, 1001, 1029
DpnI GATC 6 cut(s) 209, 320, 530, 888, 905, 985
DpnII GATC 6 cut(s) 207, 318, 528, 886, 903, 983
EaeI YGGCCR 1 cut(s) 688
Eam1104I CTCTTC 1 cut(s) 662
EarI CTCTTC 1 cut(s) 662
Eco31I GGTCTC 2 cut(s) 323, 776
Eco47I GGWCC 3 cut(s) 733, 861, 1052
Eco57I CTGAAG 2 cut(s) 270, 294
Eco81I CCTNAGG 2 cut(s) 291, 729
EcoO109I RGGNCCY 1 cut(s) 733
EcoRII CCWGG 3 cut(s) 690, 769, 874
FaeI CATG 5 cut(s) 202, 325, 476, 782, 931
FaqI GGGAC 2 cut(s) 111, 326
FatI CATG 5 cut(s) 198, 321, 472, 778, 927
FblI GTMKAC 1 cut(s) 548
Fnu4HI GCNGC 1 cut(s) 652
FokI GGATG 6 cut(s) 106, 119, 134, 206, 243, 464
Fsp4HI GCNGC 1 cut(s) 652
FspBI CTAG 3 cut(s) 69, 387, 1081
GluI GCNGC 1 cut(s) 652
GsuI CTGGAG 2 cut(s) 42, 936
HaeIII GGCC 3 cut(s) 128, 296, 690
HapII CCGG 1 cut(s) 484
Hin1II CATG 5 cut(s) 202, 325, 476, 782, 931
HincII GTYRAC 1 cut(s) 817
HindII GTYRAC 1 cut(s) 817
HindIII AAGCTT 1 cut(s) 599
HinfI GANTC 8 cut(s) 286, 313, 416, 429, 515, 764, 775, 1046
HpaII CCGG 1 cut(s) 484
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 4 cut(s) 549, 817, 864, 1066
Hpy188I TCNGA 5 cut(s) 36, 285, 318, 824, 1036
Hpy188III TCNNGA 4 cut(s) 248, 532, 779, 953
Hpy8I GTNNAC 4 cut(s) 549, 817, 864, 1066
Hpy99I CGWCG 1 cut(s) 1046
HpyAV CCTTC 2 cut(s) 97, 1018
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4V TGCA 2 cut(s) 464, 947
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 38, 365, 611
HpyF3I CTNAG 7 cut(s) 291, 315, 537, 729, 851, 1001, 1029
Hsp92II CATG 5 cut(s) 202, 325, 476, 782, 931
Kzo9I GATC 6 cut(s) 207, 318, 528, 886, 903, 983
LmnI GCTCC 2 cut(s) 37, 250
Lsp1109I GCAGC 1 cut(s) 663
LweI GCATC 3 cut(s) 19, 97, 223
MaeI CTAG 3 cut(s) 69, 387, 1081
MaeIII GTNAC 2 cut(s) 434, 584
MalI GATC 6 cut(s) 209, 320, 530, 888, 905, 985
MboI GATC 6 cut(s) 207, 318, 528, 886, 903, 983
MboII GAAGA 3 cut(s) 263, 538, 679
MfeI CAATTG 2 cut(s) 187, 660
MflI RGATCY 3 cut(s) 207, 528, 983
MhlI GDGCHC 1 cut(s) 1007
MlsI TGGCCA 1 cut(s) 690
MluCI AATT 6 cut(s) 187, 576, 660, 696, 879, 933
MluNI TGGCCA 1 cut(s) 690
MlyI GAGTC 3 cut(s) 307, 410, 438
MmeI TCCRAC 1 cut(s) 1014
Mox20I TGGCCA 1 cut(s) 690
MroXI GAANNNNTTC 1 cut(s) 1076
MscI TGGCCA 1 cut(s) 690
MseI TTAA 7 cut(s) 6, 155, 336, 509, 521, 591, 936
MslI CAYNNNNRTG 2 cut(s) 78, 758
Msp20I TGGCCA 1 cut(s) 690
MspI CCGG 1 cut(s) 484
MspR9I CCNGG 3 cut(s) 692, 771, 876
MunI CAATTG 2 cut(s) 187, 660
MvaI CCWGG 3 cut(s) 692, 771, 876
MwoI GCNNNNNNNGC 4 cut(s) 17, 38, 365, 611
NdeII GATC 6 cut(s) 207, 318, 528, 886, 903, 983
NlaIII CATG 5 cut(s) 202, 325, 476, 782, 931
NlaIV GGNNCC 2 cut(s) 862, 985
NmuCI GTSAC 2 cut(s) 434, 584
NspI RCATGY 1 cut(s) 476
OliI CACNNNNGTG 1 cut(s) 78
PagI TCATGA 1 cut(s) 778
PdmI GAANNNNTTC 1 cut(s) 1076
PfeI GAWTC 5 cut(s) 286, 515, 764, 775, 1046
PkrI GCNGC 1 cut(s) 653
PleI GAGTC 3 cut(s) 307, 410, 437
PpsI GAGTC 3 cut(s) 307, 410, 437
PpuMI RGGWCCY 1 cut(s) 733
Psp5II RGGWCCY 1 cut(s) 733
Psp6I CCWGG 3 cut(s) 690, 769, 874
PspGI CCWGG 3 cut(s) 690, 769, 874
PspN4I GGNNCC 2 cut(s) 862, 985
PspPI GGNCC 4 cut(s) 127, 733, 861, 1052
PspPPI RGGWCCY 1 cut(s) 733
PsuI RGATCY 3 cut(s) 207, 528, 983
RsaI GTAC 2 cut(s) 397, 1040
RsaNI GTAC 2 cut(s) 396, 1039
RseI CAYNNNNRTG 2 cut(s) 78, 758
SaqAI TTAA 7 cut(s) 6, 155, 336, 509, 521, 591, 936
SatI GCNGC 1 cut(s) 652
Sau3AI GATC 6 cut(s) 207, 318, 528, 886, 903, 983
Sau96I GGNCC 4 cut(s) 127, 733, 861, 1052
SchI GAGTC 3 cut(s) 307, 410, 438
ScrFI CCNGG 3 cut(s) 692, 771, 876
SduI GDGCHC 1 cut(s) 1007
SfaNI GCATC 3 cut(s) 19, 97, 223
SfcI CTRYAG 1 cut(s) 399
SinI GGWCC 3 cut(s) 733, 861, 1052
SmiMI CAYNNNNRTG 2 cut(s) 78, 758
Sse9I AATT 6 cut(s) 187, 576, 660, 696, 879, 933
SspMI CTAG 3 cut(s) 69, 387, 1081
StyD4I CCNGG 3 cut(s) 690, 769, 874
TaaI ACNGT 1 cut(s) 442
TaqI TCGA 2 cut(s) 134, 889
TasI AATT 6 cut(s) 187, 576, 660, 696, 879, 933
TfiI GAWTC 5 cut(s) 286, 515, 764, 775, 1046
Tru1I TTAA 7 cut(s) 6, 155, 336, 509, 521, 591, 936
Tru9I TTAA 7 cut(s) 6, 155, 336, 509, 521, 591, 936
TscAI CASTG 3 cut(s) 445, 859, 1005
TseFI GTSAC 2 cut(s) 434, 584
TseI GCWGC 1 cut(s) 651
Tsp45I GTSAC 2 cut(s) 434, 584
TspDTI ATGAA 6 cut(s) 539, 639, 742, 767, 818, 944
TspGWI ACGGA 2 cut(s) 710, 1044
TspRI CASTG 3 cut(s) 445, 859, 1005
VpaK11BI GGWCC 3 cut(s) 733, 861, 1052
XceI RCATGY 1 cut(s) 476
XmiI GTMKAC 1 cut(s) 548
XmnI GAANNNNTTC 1 cut(s) 1076
XspI CTAG 3 cut(s) 69, 387, 1081
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.