FvH4_5g10190

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008204
Physical Location & Seq
Reverse (-)
5827339 .. 5830827
3489 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10190.t1

Sequence Viewer

Length: 1134 bp
ATGGCCGATTCCGAGGACATTCAGCCTCTTGTCTGTGATAATGGTACTGGAATGGTTAAGGCTGGATTTGCTGGAGATGATGCCCCAAGGGCCGTCTTCCCCAGTATTGTTGGTCGACCTAGGCACACAGGTGTTATGGTGGGGATGGGACAGAAAGACGCATATGTTGGGGATGAGGCTCAGTCCAAAAGAGGTATTCTCACTTTGAAATACCCTATTGAGCATGGGATTGTTAGCAATTGGGATGACATGGAAAAAATCTGGCATCATACTTTCTACAATGAGCTACGTGTGGCTCCTGAAGAACATCCTGTGCTCCTTACTGAGGCACCCCTCAATCCTAAGGCCAACAGGGAGAAGATGACACAGATCATGTTTGAGACCTTCAATGTCCCTGCTATGTATGTTGCTATTCAAGCAGTTCTCTCCCTCTATGCCAGTGGCCGTACAACAGGTATTGTGCTGGACTCTGGTGATGGAGTCAGTCACACAGTTCCCATCTACGAAGGTTATGCACTTCCACATGCCATTCTGCGGCTTGACCTTGCTGGACGAGATTTAACAGACTCCTTGATGAAAATCCTGACCGAGAGAGGGTATTCTTTTACTACAACTGCTGAACGGGAAATTGTACGTGACATTAAAGAGAAGCTTGCATATGTGGCACTTGATTATGAACAAGAACTTGAAACTGCTTCAAGCAGCTCAACAATTGAGAAGAGCTATGAACTACCTGATGGTCAGGTAATTACCATTGGAGCAGAGCGTTTCCGTTGCCCTGAGGTCCTATTTCAACCATCACTAGTTGGAATGGAAGCAGCTGGTATTCACGAGACCACCTATAACTCGATTATGAAGTGTGATGTTGACATCAGGAAAGACTTGTATGGTAACATTGTGCTCAGTGGAGGGTCCACCATGTTCCCTGGAATCGCTGATAGGATGAGCAAGGAGATAACAGCCCTTGCACCAAGCAGCATGAAAATTAAGGTGGTGGCACCTCCAGAGAGGAAATACAGTGTATGGATCGGAGGATCTATATTGGCTTCCCTGAGTACATTCCAGCAGATGTGGATATCGAAGCCAGAGTACGATGAATCCGGGCCATCCATCGTCCACAGAAAATGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

378

Amino Acids

41.65

Weight (kDa)

5.23

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 5 - 377 3.2e-147 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 328, 997
AccI GTMKAC 1 cut(s) 115
AciI CCGC 1 cut(s) 535
AclWI GGATC 2 cut(s) 1034, 1042
AcoI YGGCCR 2 cut(s) 3, 442
AcuI CTGAAG 1 cut(s) 321
AfaI GTAC 5 cut(s) 46, 448, 633, 1057, 1091
AfiI CCNNNNNNNGG 3 cut(s) 325, 534, 594
AflIII ACRYGT 1 cut(s) 289
AgsI TTSAA 6 cut(s) 208, 388, 416, 689, 699, 794
AhlI ACTAGT 1 cut(s) 802
AjnI CCWGG 1 cut(s) 925
AleI CACNNNNGTG 1 cut(s) 129
AluBI AGCT 5 cut(s) 286, 652, 705, 723, 821
AluI AGCT 5 cut(s) 286, 652, 705, 723, 821
Alw21I GWGCWC 2 cut(s) 318, 903
Alw26I GTCTC 2 cut(s) 374, 827
AlwI GGATC 2 cut(s) 1034, 1042
AoxI GGCC 5 cut(s) 3, 90, 345, 442, 1103
ApeKI GCWGC 3 cut(s) 702, 818, 975
AspA2I CCTAGG 1 cut(s) 119
AspS9I GGNCC 4 cut(s) 90, 784, 912, 1103
AsuC2I CCSGG 1 cut(s) 1102
AsuHPI GGTGA 1 cut(s) 485
AvaII GGWCC 2 cut(s) 784, 912
AvrII CCTAGG 1 cut(s) 119
AxyI CCTNAGG 2 cut(s) 342, 780
BanI GGYRCC 2 cut(s) 328, 997
BarI GAAGNNNNNNTAC 2 cut(s) 1073, 1105
BauI CACGAG 1 cut(s) 830
BbsI GAAGAC 1 cut(s) 88
Bbv12I GWGCWC 2 cut(s) 318, 903
BbvI GCAGC 3 cut(s) 714, 830, 987
BccI CCATC 7 cut(s) 139, 470, 506, 731, 805, 1114, 1118
BceAI ACGGC 2 cut(s) 77, 429
BcgI CGANNNNNNTGC 2 cut(s) 494, 528
BciT130I CCWGG 1 cut(s) 927
BcnI CCSGG 1 cut(s) 1102
BcoDI GTCTC 2 cut(s) 374, 827
BcuI ACTAGT 1 cut(s) 802
BfaI CTAG 3 cut(s) 120, 803, 1132
BglI GCCNNNNNGGC 1 cut(s) 89
BisI GCNGC 4 cut(s) 536, 703, 819, 976
BlnI CCTAGG 1 cut(s) 119
BlsI GCNGC 4 cut(s) 537, 704, 820, 977
Bme1390I CCNGG 2 cut(s) 927, 1102
Bme18I GGWCC 2 cut(s) 784, 912
BmgT120I GGNCC 4 cut(s) 90, 784, 912, 1103
BmiI GGNNCC 4 cut(s) 297, 330, 913, 999
BmrFI CCNGG 2 cut(s) 927, 1102
BmrI ACTGGG 1 cut(s) 96
BmsI GCATC 2 cut(s) 70, 274
BmuI ACTGGG 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 88
BplI GAGNNNNNCTC 2 cut(s) 183, 215
BpmI CTGGAG 2 cut(s) 93, 987
BpuMI CCSGG 1 cut(s) 1102
BsaAI YACGTR 2 cut(s) 290, 635
BsaBI GATNNNNATC 1 cut(s) 578
BsaI GGTCTC 2 cut(s) 374, 827
BsaJI CCNNGG 4 cut(s) 12, 86, 119, 925
Bsc4I CCNNNNNNNGG 3 cut(s) 325, 534, 594
Bse1I ACTGG 3 cut(s) 52, 102, 438
Bse21I CCTNAGG 2 cut(s) 342, 780
Bse8I GATNNNNATC 1 cut(s) 578
BseBI CCWGG 1 cut(s) 927
BseDI CCNNGG 4 cut(s) 12, 86, 119, 925
BseGI GGATG 6 cut(s) 150, 178, 250, 307, 948, 1106
BseJI GATNNNNATC 1 cut(s) 578
BseLI CCNNNNNNNGG 3 cut(s) 325, 534, 594
BseMII CTCAG 5 cut(s) 194, 315, 771, 916, 1043
BseNI ACTGG 3 cut(s) 52, 102, 438
BseXI GCAGC 3 cut(s) 714, 830, 987
BshFI GGCC 5 cut(s) 5, 92, 347, 444, 1105
BshNI GGYRCC 2 cut(s) 328, 997
BsiHKAI GWGCWC 2 cut(s) 318, 903
BsiSI CCGG 1 cut(s) 1101
BslFI GGGAC 2 cut(s) 162, 377
BslI CCNNNNNNNGG 3 cut(s) 325, 534, 594
BsmAI GTCTC 2 cut(s) 374, 827
BsmFI GGGAC 2 cut(s) 162, 377
BsnI GGCC 5 cut(s) 5, 92, 347, 444, 1105
Bso31I GGTCTC 2 cut(s) 374, 827
Bsp1286I GDGCHC 2 cut(s) 318, 903
Bsp143I GATC 3 cut(s) 369, 1026, 1034
BspACI CCGC 1 cut(s) 535
BspANI GGCC 5 cut(s) 5, 92, 347, 444, 1105
BspCNI CTCAG 5 cut(s) 193, 316, 772, 915, 1044
BspLI GGNNCC 4 cut(s) 297, 330, 913, 999
BspPI GGATC 2 cut(s) 1034, 1042
BspQI GCTCTTC 1 cut(s) 713
BspT107I GGYRCC 2 cut(s) 328, 997
BspTNI GGTCTC 2 cut(s) 374, 827
BsrI ACTGG 3 cut(s) 52, 102, 438
BssECI CCNNGG 4 cut(s) 12, 86, 119, 925
BssMI GATC 3 cut(s) 369, 1026, 1034
BssSI CACGAG 1 cut(s) 830
BssT1I CCWWGG 2 cut(s) 86, 119
Bst2BI CACGAG 1 cut(s) 830
Bst2UI CCWGG 1 cut(s) 927
Bst4CI ACNGT 2 cut(s) 493, 1019
Bst6I CTCTTC 1 cut(s) 713
BstBAI YACGTR 2 cut(s) 290, 635
BstC8I GCNNGC 1 cut(s) 654
BstDEI CTNAG 6 cut(s) 180, 324, 342, 780, 902, 1052
BstENI CCTNNNNNAGG 1 cut(s) 323
BstF5I GGATG 6 cut(s) 150, 178, 250, 307, 948, 1106
BstKTI GATC 3 cut(s) 372, 1029, 1037
BstMAI GTCTC 2 cut(s) 374, 827
BstMBI GATC 3 cut(s) 369, 1026, 1034
BstMWI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
BstNI CCWGG 1 cut(s) 927
BstNSI RCATGY 1 cut(s) 527
BstSCI CCNGG 2 cut(s) 925, 1100
BstV1I GCAGC 3 cut(s) 714, 830, 987
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 1 cut(s) 1034
BstYI RGATCY 1 cut(s) 1034
Bsu36I CCTNAGG 2 cut(s) 342, 780
BsuRI GGCC 5 cut(s) 5, 92, 347, 444, 1105
BtsCI GGATG 6 cut(s) 150, 178, 250, 307, 948, 1106
BtsIMutI CAGTG 3 cut(s) 445, 910, 1024
Cac8I GCNNGC 1 cut(s) 654
Cfr13I GGNCC 4 cut(s) 90, 784, 912, 1103
CseI GACGC 1 cut(s) 167
Csp6I GTAC 5 cut(s) 45, 447, 632, 1056, 1090
CviAII CATG 6 cut(s) 224, 250, 373, 524, 919, 979
CviQI GTAC 5 cut(s) 45, 447, 632, 1056, 1090
DdeI CTNAG 6 cut(s) 180, 324, 342, 780, 902, 1052
DpnI GATC 3 cut(s) 371, 1028, 1036
DpnII GATC 3 cut(s) 369, 1026, 1034
EaeI YGGCCR 2 cut(s) 3, 442
Eam1104I CTCTTC 1 cut(s) 713
EarI CTCTTC 1 cut(s) 713
Eco130I CCWWGG 2 cut(s) 86, 119
Eco31I GGTCTC 2 cut(s) 374, 827
Eco32I GATATC 1 cut(s) 1077
Eco47I GGWCC 2 cut(s) 784, 912
Eco57I CTGAAG 1 cut(s) 321
Eco81I CCTNAGG 2 cut(s) 342, 780
EcoNI CCTNNNNNAGG 1 cut(s) 323
EcoO109I RGGNCCY 1 cut(s) 784
EcoRII CCWGG 1 cut(s) 925
EcoRV GATATC 1 cut(s) 1077
EcoT14I CCWWGG 2 cut(s) 86, 119
ErhI CCWWGG 2 cut(s) 86, 119
FaeI CATG 6 cut(s) 227, 253, 376, 527, 922, 982
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FaqI GGGAC 2 cut(s) 162, 377
FatI CATG 6 cut(s) 223, 249, 372, 523, 918, 978
FauNDI CATATG 2 cut(s) 163, 658
FblI GTMKAC 1 cut(s) 115
Fnu4HI GCNGC 4 cut(s) 536, 703, 819, 976
FokI GGATG 6 cut(s) 157, 185, 257, 294, 955, 1093
Fsp4HI GCNGC 4 cut(s) 536, 703, 819, 976
FspBI CTAG 3 cut(s) 120, 803, 1132
GluI GCNGC 4 cut(s) 536, 703, 819, 976
GsuI CTGGAG 2 cut(s) 93, 987
HaeIII GGCC 5 cut(s) 5, 92, 347, 444, 1105
HapII CCGG 1 cut(s) 1101
HgaI GACGC 1 cut(s) 167
Hin1II CATG 6 cut(s) 227, 253, 376, 527, 922, 982
HincII GTYRAC 2 cut(s) 116, 868
HindII GTYRAC 2 cut(s) 116, 868
HindIII AAGCTT 1 cut(s) 650
HinfI GANTC 6 cut(s) 8, 467, 480, 566, 930, 1097
HpaII CCGG 1 cut(s) 1101
HphI GGTGA 1 cut(s) 485
Hpy166II GTNNAC 4 cut(s) 116, 868, 915, 1117
Hpy188I TCNGA 2 cut(s) 13, 1031
Hpy188III TCNNGA 5 cut(s) 299, 583, 830, 874, 1004
Hpy8I GTNNAC 4 cut(s) 116, 868, 915, 1117
HpyAV CCTTC 2 cut(s) 394, 500
HpyCH4III ACNGT 2 cut(s) 493, 1019
HpyCH4IV ACGT 2 cut(s) 289, 634
HpyCH4V TGCA 3 cut(s) 515, 656, 968
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
HpyF3I CTNAG 6 cut(s) 180, 324, 342, 780, 902, 1052
HpySE526I ACGT 2 cut(s) 289, 634
Hsp92II CATG 6 cut(s) 227, 253, 376, 527, 922, 982
Kzo9I GATC 3 cut(s) 369, 1026, 1034
LguI GCTCTTC 1 cut(s) 713
LmnI GCTCC 3 cut(s) 301, 321, 758
Lsp1109I GCAGC 3 cut(s) 714, 830, 987
LweI GCATC 2 cut(s) 70, 274
MaeI CTAG 3 cut(s) 120, 803, 1132
MaeII ACGT 2 cut(s) 289, 634
MaeIII GTNAC 3 cut(s) 485, 635, 890
MalI GATC 3 cut(s) 371, 1028, 1036
MboI GATC 3 cut(s) 369, 1026, 1034
MboII GAAGA 4 cut(s) 88, 314, 370, 730
MfeI CAATTG 2 cut(s) 238, 711
MflI RGATCY 1 cut(s) 1034
MhlI GDGCHC 2 cut(s) 318, 903
MluCI AATT 5 cut(s) 238, 627, 711, 747, 984
MlyI GAGTC 3 cut(s) 461, 489, 560
MmeI TCCRAC 1 cut(s) 787
MseI TTAA 4 cut(s) 57, 560, 642, 987
MslI CAYNNNNRTG 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 821
MspI CCGG 1 cut(s) 1101
MspR9I CCNGG 2 cut(s) 927, 1102
MunI CAATTG 2 cut(s) 238, 711
MvaI CCWGG 1 cut(s) 927
MwoI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
NciI CCSGG 1 cut(s) 1102
NdeI CATATG 2 cut(s) 163, 658
NdeII GATC 3 cut(s) 369, 1026, 1034
NlaIII CATG 6 cut(s) 227, 253, 376, 527, 922, 982
NlaIV GGNNCC 4 cut(s) 297, 330, 913, 999
NmuCI GTSAC 2 cut(s) 485, 635
NspI RCATGY 1 cut(s) 527
OliI CACNNNNGTG 1 cut(s) 129
PciSI GCTCTTC 1 cut(s) 713
PfeI GAWTC 3 cut(s) 8, 930, 1097
PkrI GCNGC 4 cut(s) 537, 704, 820, 977
PleI GAGTC 3 cut(s) 461, 488, 560
PpsI GAGTC 3 cut(s) 461, 488, 560
Ppu21I YACGTR 2 cut(s) 290, 635
PpuMI RGGWCCY 1 cut(s) 784
Psp5II RGGWCCY 1 cut(s) 784
Psp6I CCWGG 1 cut(s) 925
PspGI CCWGG 1 cut(s) 925
PspN4I GGNNCC 4 cut(s) 297, 330, 913, 999
PspPI GGNCC 4 cut(s) 90, 784, 912, 1103
PspPPI RGGWCCY 1 cut(s) 784
PsuI RGATCY 1 cut(s) 1034
PvuII CAGCTG 1 cut(s) 821
RsaI GTAC 5 cut(s) 46, 448, 633, 1057, 1091
RsaNI GTAC 5 cut(s) 45, 447, 632, 1056, 1090
RseI CAYNNNNRTG 1 cut(s) 129
SalI GTCGAC 1 cut(s) 114
SapI GCTCTTC 1 cut(s) 713
SaqAI TTAA 4 cut(s) 57, 560, 642, 987
SatI GCNGC 4 cut(s) 536, 703, 819, 976
Sau3AI GATC 3 cut(s) 369, 1026, 1034
Sau96I GGNCC 4 cut(s) 90, 784, 912, 1103
SchI GAGTC 3 cut(s) 461, 489, 560
ScrFI CCNGG 2 cut(s) 927, 1102
SduI GDGCHC 2 cut(s) 318, 903
SfaNI GCATC 2 cut(s) 70, 274
SinI GGWCC 2 cut(s) 784, 912
SmiMI CAYNNNNRTG 1 cut(s) 129
SpeI ACTAGT 1 cut(s) 802
Sse9I AATT 5 cut(s) 238, 627, 711, 747, 984
SsiI CCGC 1 cut(s) 535
SspMI CTAG 3 cut(s) 120, 803, 1132
StyD4I CCNGG 2 cut(s) 925, 1100
StyI CCWWGG 2 cut(s) 86, 119
TaaI ACNGT 2 cut(s) 493, 1019
TaiI ACGT 2 cut(s) 292, 637
TaqI TCGA 3 cut(s) 115, 848, 1079
TaqII GACCGA 1 cut(s) 602
TasI AATT 5 cut(s) 238, 627, 711, 747, 984
TatI WGTACW 1 cut(s) 1055
TauI GCSGC 1 cut(s) 538
TfiI GAWTC 3 cut(s) 8, 930, 1097
Tru1I TTAA 4 cut(s) 57, 560, 642, 987
Tru9I TTAA 4 cut(s) 57, 560, 642, 987
TscAI CASTG 3 cut(s) 445, 910, 1024
TseFI GTSAC 2 cut(s) 485, 635
TseI GCWGC 3 cut(s) 702, 818, 975
Tsp45I GTSAC 2 cut(s) 485, 635
TspDTI ATGAA 6 cut(s) 590, 690, 741, 869, 995, 1110
TspGWI ACGGA 1 cut(s) 761
TspRI CASTG 3 cut(s) 445, 910, 1024
VpaK11BI GGWCC 2 cut(s) 784, 912
XagI CCTNNNNNAGG 1 cut(s) 323
XceI RCATGY 1 cut(s) 527
XmaJI CCTAGG 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 115
XspI CTAG 3 cut(s) 120, 803, 1132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.