AT2G43030

Belongs to the universal ribosomal protein uL3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015186
Physical Location & Seq
Forward (+)
17894796 .. 17895882
1087 bp
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UTR
Exon/CDS
Intron
AT2G43030.1

Sequence Viewer

Length: 816 bp
ATGGCGATAGCTATGGCGGTTGTTTCTTTCCCATCTCTGCTCAACAAAACAACCTTATCCTCTTCACTCTTCACTCCCACTTTCCTCCCGGCGAAATCCTCTTCACTTCTCATCAAATCATCACCAAAAACCAGATTCGTGGTTTCATCATCCATGGAAGCTGGAATCGGAGTAATGGGGTCAAAACTCGGGATGATGAGTTTCTTCGAAGAAGACGGCACAGTAGTTCCAGTAACAGTCGTCGGATTCCGCGAAGGTAACATCGTAACCCAAATCAAAACCCTAGCCACAGACGGTTACGACGCGGTTCAAATCGGTTACCGTCGTGTACGCGACAAAAAGCTAACTAAACCGGAAACCGGTCATCTCCAAAAAGCCGGTACAATCCCAATGAGACATCTCCAAGAGTTCAGATTAACAAACATCGAAGGATTCGAACCGAACCAGAAGCTTGTGTTCGATGAGATATTCAAAGAAGGTGATCTCGTTGATGTAGCTGGAACCACAATTGGTAAAGGGTTTCAAGGAGGAATCAAAAGGCATCATTTCAAGAGAGGTCAGATGACTCATGGTTCCAAGAGTCATCGAGCTTTGGGTTCGATTGGTGCTGGTACTACTCCGGGAAGAGTTTATAAAGGGAAGAAGATGCCTGGAAGAATGGGAGGGACGAGGACAAAGATTAGGAAGCTTAAGATTGTTAAAGTTGATAAGGAGCTTAATGTTGTCATGATCAAAGGTGCTTTGCCTGGTAAGCCTGGGAATCTTCTTCGTATTACTCCCGCTAAAATCGTCGGAGTTAACATTCCCAAGAACTAG

Protein Analysis

271

Amino Acids

29.36

Weight (kDa)

10.43

Isoelectric Point (pI)

31.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 150 - 237 3e-15 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43030
fragaria_vesca FvH4_7g08200
malus_domestica MD02G1230700.v1.1 MD07G1082200.v1.1
prunus_persica Prupe.2G107400_v2.0.a1
pyrus_communis pycom02g19980 pycom07g06550
rosa_chinensis RchiOBHm_Chr1g0340951
rosa_laevigata RLG00000029053
rosa_multiflora Rmu_sc0002200.1_g000047
rosa_roxburghii Rroxscaffold_4G00311600
rosa_rugosa Rorug01G0160600.1
rosa_samantha Rh1AG175300 Rh1BG143600 Rh1DG175200
rosa_wichuraiana Rw1G014690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 633
AccII CGCG 3 cut(s) 252, 305, 333
AciI CCGC 4 cut(s) 17, 250, 305, 780
AfaI GTAC 3 cut(s) 330, 382, 613
AfiI CCNNNNNNNGG 1 cut(s) 359
AflII CTTAAG 1 cut(s) 689
AgeI ACCGGT 1 cut(s) 359
AgsI TTSAA 4 cut(s) 311, 472, 524, 550
AjnI CCWGG 3 cut(s) 649, 745, 754
AluBI AGCT 8 cut(s) 11, 161, 343, 451, 497, 590, 688, 715
AluI AGCT 8 cut(s) 11, 161, 343, 451, 497, 590, 688, 715
Alw26I GTCTC 1 cut(s) 388
Ama87I CYCGRG 1 cut(s) 188
AsiGI ACCGGT 1 cut(s) 359
AsuC2I CCSGG 2 cut(s) 89, 621
AsuHPI GGTGA 2 cut(s) 114, 491
AsuII TTCGAA 2 cut(s) 207, 435
AvaI CYCGRG 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 219
BccI CCATC 1 cut(s) 40
BceAI ACGGC 1 cut(s) 232
BciT130I CCWGG 3 cut(s) 651, 747, 756
BclI TGATCA 1 cut(s) 729
BcnI CCSGG 2 cut(s) 89, 621
BcoDI GTCTC 1 cut(s) 388
BfaI CTAG 2 cut(s) 284, 814
BfrI CTTAAG 1 cut(s) 689
Bme1390I CCNGG 5 cut(s) 89, 621, 651, 747, 756
BmeT110I CYCGRG 1 cut(s) 188
BmiI GGNNCC 2 cut(s) 502, 574
BmrFI CCNGG 5 cut(s) 89, 621, 651, 747, 756
BmsI GCATC 2 cut(s) 550, 636
BpiI GAAGAC 1 cut(s) 219
Bpu14I TTCGAA 2 cut(s) 207, 435
BpuMI CCSGG 2 cut(s) 89, 621
BsaJI CCNNGG 2 cut(s) 153, 755
BsaWI WCCGGW 2 cut(s) 352, 359
Bsc4I CCNNNNNNNGG 1 cut(s) 359
Bse118I RCCGGY 2 cut(s) 359, 377
Bse1I ACTGG 1 cut(s) 230
BseBI CCWGG 3 cut(s) 651, 747, 756
BseDI CCNNGG 2 cut(s) 153, 755
BseGI GGATG 2 cut(s) 149, 198
BseLI CCNNNNNNNGG 1 cut(s) 359
BseNI ACTGG 1 cut(s) 230
Bsh1236I CGCG 3 cut(s) 252, 305, 333
BshTI ACCGGT 1 cut(s) 359
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 5 cut(s) 89, 353, 360, 378, 620
BslFI GGGAC 1 cut(s) 679
BslI CCNNNNNNNGG 1 cut(s) 359
BsmAI GTCTC 1 cut(s) 388
BsmFI GGGAC 1 cut(s) 679
BsoBI CYCGRG 1 cut(s) 188
Bsp119I TTCGAA 2 cut(s) 207, 435
Bsp143I GATC 2 cut(s) 481, 729
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 4 cut(s) 17, 250, 305, 780
BspFNI CGCG 3 cut(s) 252, 305, 333
BspHI TCATGA 1 cut(s) 726
BspLI GGNNCC 2 cut(s) 502, 574
BspT104I TTCGAA 2 cut(s) 207, 435
BspTI CTTAAG 1 cut(s) 689
BsrFI RCCGGY 2 cut(s) 359, 377
BsrI ACTGG 1 cut(s) 230
BssAI RCCGGY 2 cut(s) 359, 377
BssECI CCNNGG 2 cut(s) 153, 755
BssMI GATC 2 cut(s) 481, 729
BssT1I CCWWGG 1 cut(s) 153
Bst2UI CCWGG 3 cut(s) 651, 747, 756
Bst4CI ACNGT 4 cut(s) 223, 238, 296, 323
Bst6I CTCTTC 4 cut(s) 67, 74, 106, 619
BstAFI CTTAAG 1 cut(s) 689
BstBI TTCGAA 2 cut(s) 207, 435
BstDSI CCRYGG 1 cut(s) 153
BstEII GGTNACC 1 cut(s) 317
BstF5I GGATG 2 cut(s) 149, 198
BstFNI CGCG 3 cut(s) 252, 305, 333
BstKTI GATC 2 cut(s) 484, 732
BstMAI GTCTC 1 cut(s) 388
BstMBI GATC 2 cut(s) 481, 729
BstMWI GCNNNNNNNGC 1 cut(s) 751
BstNI CCWGG 3 cut(s) 651, 747, 756
BstPI GGTNACC 1 cut(s) 317
BstSCI CCNGG 5 cut(s) 87, 619, 649, 745, 754
BstUI CGCG 3 cut(s) 252, 305, 333
BstV2I GAAGAC 1 cut(s) 219
BstXI CCANNNNNNTGG 1 cut(s) 139
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 2 cut(s) 149, 198
CciI TCATGA 1 cut(s) 726
Cfr10I RCCGGY 2 cut(s) 359, 377
CseI GACGC 1 cut(s) 311
Csp6I GTAC 3 cut(s) 329, 381, 612
CspAI ACCGGT 1 cut(s) 359
CviAII CATG 3 cut(s) 154, 569, 727
CviQI GTAC 3 cut(s) 329, 381, 612
DpnI GATC 2 cut(s) 483, 731
DpnII GATC 2 cut(s) 481, 729
Eam1104I CTCTTC 4 cut(s) 67, 74, 106, 619
EarI CTCTTC 4 cut(s) 67, 74, 106, 619
Eco130I CCWWGG 1 cut(s) 153
Eco88I CYCGRG 1 cut(s) 188
Eco91I GGTNACC 1 cut(s) 317
EcoO65I GGTNACC 1 cut(s) 317
EcoRII CCWGG 3 cut(s) 649, 745, 754
EcoT14I CCWWGG 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 153
FaeI CATG 3 cut(s) 157, 572, 730
FaiI YATR 5 cut(s) 14, 155, 570, 633, 728
FaqI GGGAC 1 cut(s) 679
FatI CATG 3 cut(s) 153, 568, 726
FauI CCCGC 1 cut(s) 787
FbaI TGATCA 1 cut(s) 729
FokI GGATG 2 cut(s) 136, 205
FspBI CTAG 2 cut(s) 284, 814
HapII CCGG 5 cut(s) 89, 353, 360, 378, 620
HgaI GACGC 1 cut(s) 311
Hin1II CATG 3 cut(s) 157, 572, 730
HincII GTYRAC 1 cut(s) 799
HindII GTYRAC 1 cut(s) 799
HindIII AAGCTT 2 cut(s) 449, 686
HinfI GANTC 8 cut(s) 135, 165, 246, 432, 531, 565, 580, 760
HpaI GTTAAC 1 cut(s) 799
HpaII CCGG 5 cut(s) 89, 353, 360, 378, 620
HphI GGTGA 2 cut(s) 114, 491
Hpy166II GTNNAC 2 cut(s) 329, 799
Hpy188I TCNGA 5 cut(s) 170, 245, 413, 561, 794
Hpy188III TCNNGA 3 cut(s) 190, 550, 727
Hpy8I GTNNAC 2 cut(s) 329, 799
Hpy99I CGWCG 4 cut(s) 245, 305, 327, 794
HpyAV CCTTC 3 cut(s) 248, 422, 470
HpyCH4III ACNGT 4 cut(s) 223, 238, 296, 323
HpyF10VI GCNNNNNNNGC 1 cut(s) 751
Hsp92II CATG 3 cut(s) 157, 572, 730
Ksp22I TGATCA 1 cut(s) 729
KspAI GTTAAC 1 cut(s) 799
Kzo9I GATC 2 cut(s) 481, 729
LmnI GCTCC 1 cut(s) 712
LweI GCATC 2 cut(s) 550, 636
MaeI CTAG 2 cut(s) 284, 814
MaeIII GTNAC 5 cut(s) 232, 257, 265, 296, 317
MalI GATC 2 cut(s) 483, 731
MboI GATC 2 cut(s) 481, 729
MfeI CAATTG 1 cut(s) 507
MluCI AATT 1 cut(s) 507
MlyI GAGTC 2 cut(s) 559, 589
MmeI TCCRAC 2 cut(s) 223, 772
MnlI CCTC 7 cut(s) 70, 95, 109, 521, 548, 656, 663
MseI TTAA 5 cut(s) 416, 690, 699, 717, 798
MspCI CTTAAG 1 cut(s) 689
MspI CCGG 5 cut(s) 89, 353, 360, 378, 620
MspR9I CCNGG 5 cut(s) 89, 621, 651, 747, 756
MunI CAATTG 1 cut(s) 507
MvaI CCWGG 3 cut(s) 651, 747, 756
MvnI CGCG 3 cut(s) 252, 305, 333
MwoI GCNNNNNNNGC 1 cut(s) 751
NciI CCSGG 2 cut(s) 89, 621
NcoI CCATGG 1 cut(s) 153
NdeII GATC 2 cut(s) 481, 729
NlaIII CATG 3 cut(s) 157, 572, 730
NlaIV GGNNCC 2 cut(s) 502, 574
NspV TTCGAA 2 cut(s) 207, 435
PagI TCATGA 1 cut(s) 726
PcsI WCGNNNNNNNCGW 2 cut(s) 249, 432
PfeI GAWTC 6 cut(s) 135, 165, 246, 432, 531, 760
PfoI TCCNGGA 1 cut(s) 619
PinAI ACCGGT 1 cut(s) 359
PleI GAGTC 2 cut(s) 559, 588
PpsI GAGTC 2 cut(s) 559, 588
PsiI TTATAA 1 cut(s) 633
Psp6I CCWGG 3 cut(s) 649, 745, 754
PspEI GGTNACC 1 cut(s) 317
PspGI CCWGG 3 cut(s) 649, 745, 754
PspN4I GGNNCC 2 cut(s) 502, 574
RsaI GTAC 3 cut(s) 330, 382, 613
RsaNI GTAC 3 cut(s) 329, 381, 612
SaqAI TTAA 5 cut(s) 416, 690, 699, 717, 798
Sau3AI GATC 2 cut(s) 481, 729
SchI GAGTC 2 cut(s) 559, 589
ScrFI CCNGG 5 cut(s) 89, 621, 651, 747, 756
SfaNI GCATC 2 cut(s) 550, 636
SfuI TTCGAA 2 cut(s) 207, 435
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
Sse9I AATT 1 cut(s) 507
SsiI CCGC 4 cut(s) 17, 250, 305, 780
SspMI CTAG 2 cut(s) 284, 814
StyD4I CCNGG 5 cut(s) 87, 619, 649, 745, 754
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 4 cut(s) 223, 238, 296, 323
TaqI TCGA 6 cut(s) 207, 426, 435, 459, 586, 599
TasI AATT 1 cut(s) 507
TfiI GAWTC 6 cut(s) 135, 165, 246, 432, 531, 760
Tru1I TTAA 5 cut(s) 416, 690, 699, 717, 798
Tru9I TTAA 5 cut(s) 416, 690, 699, 717, 798
TspDTI ATGAA 1 cut(s) 135
Vha464I CTTAAG 1 cut(s) 689
XspI CTAG 2 cut(s) 284, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.