RchiOBHm_Chr1g0340951

Belongs to the universal ribosomal protein uL3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0015186
Physical Location & Seq
Reverse (-)
32609640 .. 32610954
1315 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56771

Sequence Viewer

Length: 927 bp
ATGGAGTTGGGGTGGTCGTGCTTATCAGTAGATTGCCACTCTCCCAGACCCAATTCCTATAAGGATAAGACCAGTCTTCTTCTTCTTCACTCCGAACTTGTGCTCTCTATAGAGAGAGAACCAAACCCAAAAATGGCACTTCTTCCAATTCAAACTGCTCTCCTTCCCAATCTCAAACCCTCTCCCTTTCTCACACCCAAAAACCCATTCCCCCTCCTCACCAAAACCCACAAGAAGCCCCACTCCACAGTCACCATGATGAGCAAGGAGGCCGGCATCGGCGTCATGGCCACCAAGCTCGGCATGATGAGCTTCTTCGAACCCAACGGCAAGGTCGTCCCCGTCACCGTCGTCGGCTTCAAAGAAGGCAACATTGTCACCCAAATCAAAACCCAAGCCACCGACGGCTACGACGCCGTTCAAGTCGGCTACCGCCGCATCCGCGACCGGAAACTCACCAAGCCCGAAATGGGCCATCTCAACAAGTCCGGCGCAATCCCTCTCCGCCACCTCCAGGAGTTTCGTCTTCAGGACATTGAAGGGTTTGAGCCCAACCAGAAGCTTGATTTCAGCGAGCTCTTCAAGGAGGGTGACCTCGTCGACGTCGCCGGAACCACGATCGGGAAAGGGTTTCAGGGTGGGATTAAGAGGCACCATTTCAAGAGGGGGCTGATGACTCATGGGTCCAAGAGTCACAGACAGCTTGGGTCGATTGGGGCCGGAACCACGCCCGGGAGGGTTTACAAGGGGAAGAAGATGCCTGGGAGAATGGGAGGGACTAAGACCAAGATCAGGAAGCTCAAGATTGTCAAGATTGATAAGGACCTCAATGTGGTTATGATTAATGGTGCTCTTCCTGGTAAGCCTGGGAACCTTCTCCGCATTACTCCGGCTAAGATTGTAGGGAAGAATATCCCTAAGAGTTAA

Protein Analysis

308

Amino Acids

33.91

Weight (kDa)

10.15

Isoelectric Point (pI)

32.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 187 - 274 1.1e-15 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43030
fragaria_vesca FvH4_7g08200
malus_domestica MD02G1230700.v1.1 MD07G1082200.v1.1
prunus_persica Prupe.2G107400_v2.0.a1
pyrus_communis pycom02g19980 pycom07g06550
rosa_chinensis RchiOBHm_Chr1g0340951
rosa_laevigata RLG00000029053
rosa_multiflora Rmu_sc0002200.1_g000047
rosa_roxburghii Rroxscaffold_4G00311600
rosa_rugosa Rorug01G0160600.1
rosa_samantha Rh1AG175300 Rh1BG143600 Rh1DG175200
rosa_wichuraiana Rw1G014690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 682
AatII GACGTC 1 cut(s) 606
AccB1I GGYRCC 1 cut(s) 651
AccI GTMKAC 1 cut(s) 600
AccII CGCG 1 cut(s) 444
AciI CCGC 5 cut(s) 433, 436, 442, 505, 880
AcoI YGGCCR 1 cut(s) 288
AcuI CTGAAG 1 cut(s) 512
AcyI GRCGYC 3 cut(s) 282, 414, 603
AfiI CCNNNNNNNGG 7 cut(s) 133, 470, 514, 621, 732, 792, 832
AgsI TTSAA 6 cut(s) 152, 361, 422, 539, 583, 661
AjnI CCWGG 4 cut(s) 513, 760, 856, 865
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AluBI AGCT 6 cut(s) 298, 312, 562, 577, 703, 799
AluI AGCT 6 cut(s) 298, 312, 562, 577, 703, 799
Alw21I GWGCWC 3 cut(s) 105, 579, 853
Ama87I CYCGRG 1 cut(s) 731
AoxI GGCC 4 cut(s) 270, 288, 472, 717
AseI ATTAAT 1 cut(s) 843
AspLEI GCGC 1 cut(s) 494
AspS9I GGNCC 4 cut(s) 472, 684, 717, 823
AsuC2I CCSGG 2 cut(s) 732, 733
AsuHPI GGTGA 6 cut(s) 211, 244, 337, 370, 448, 602
AsuII TTCGAA 1 cut(s) 318
AvaI CYCGRG 1 cut(s) 731
AvaII GGWCC 2 cut(s) 684, 823
BalI TGGCCA 1 cut(s) 290
BanI GGYRCC 1 cut(s) 651
BanII GRGCYC 2 cut(s) 552, 579
BbsI GAAGAC 2 cut(s) 68, 518
Bbv12I GWGCWC 3 cut(s) 105, 579, 853
BccI CCATC 1 cut(s) 483
BceAI ACGGC 3 cut(s) 343, 401, 421
BciT130I CCWGG 4 cut(s) 515, 762, 858, 867
BcnI CCSGG 2 cut(s) 732, 733
BfmI CTRYAG 1 cut(s) 108
BisI GCNGC 1 cut(s) 436
BlsI GCNGC 1 cut(s) 437
Bme1390I CCNGG 6 cut(s) 515, 732, 733, 762, 858, 867
Bme18I GGWCC 2 cut(s) 684, 823
BmeT110I CYCGRG 1 cut(s) 731
BmgT120I GGNCC 4 cut(s) 472, 684, 717, 823
BmiI GGNNCC 6 cut(s) 613, 653, 685, 718, 724, 872
BmrFI CCNGG 6 cut(s) 515, 732, 733, 762, 858, 867
BmsI GCATC 3 cut(s) 285, 447, 747
BpiI GAAGAC 2 cut(s) 68, 518
BpmI CTGGAG 1 cut(s) 497
Bpu14I TTCGAA 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 785
BpuMI CCSGG 2 cut(s) 732, 733
BsaHI GRCGYC 3 cut(s) 282, 414, 603
BsaJI CCNNGG 3 cut(s) 731, 761, 866
BsaWI WCCGGW 1 cut(s) 447
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 7 cut(s) 133, 470, 514, 621, 732, 792, 832
Bse118I RCCGGY 1 cut(s) 272
Bse1I ACTGG 1 cut(s) 72
BseBI CCWGG 4 cut(s) 515, 762, 858, 867
BseDI CCNNGG 3 cut(s) 731, 761, 866
BseGI GGATG 1 cut(s) 438
BseLI CCNNNNNNNGG 7 cut(s) 133, 470, 514, 621, 732, 792, 832
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 1 cut(s) 206
Bsh1236I CGCG 1 cut(s) 444
Bsh1285I CGRYCG 2 cut(s) 448, 621
BshFI GGCC 4 cut(s) 272, 290, 474, 719
BshNI GGYRCC 1 cut(s) 651
BsiEI CGRYCG 2 cut(s) 448, 621
BsiHKAI GWGCWC 3 cut(s) 105, 579, 853
BsiHKCI CYCGRG 1 cut(s) 731
BsiSI CCGG 7 cut(s) 273, 448, 489, 609, 720, 732, 890
BslFI GGGAC 2 cut(s) 323, 790
BslI CCNNNNNNNGG 7 cut(s) 133, 470, 514, 621, 732, 792, 832
BsmFI GGGAC 2 cut(s) 323, 790
BsnI GGCC 4 cut(s) 272, 290, 474, 719
BsoBI CYCGRG 1 cut(s) 731
Bsp119I TTCGAA 1 cut(s) 318
Bsp1286I GDGCHC 4 cut(s) 105, 552, 579, 853
Bsp143I GATC 2 cut(s) 618, 789
BspACI CCGC 5 cut(s) 433, 436, 442, 505, 880
BspANI GGCC 4 cut(s) 272, 290, 474, 719
BspFNI CGCG 1 cut(s) 444
BspLI GGNNCC 6 cut(s) 613, 653, 685, 718, 724, 872
BspQI GCTCTTC 2 cut(s) 584, 858
BspT104I TTCGAA 1 cut(s) 318
BspT107I GGYRCC 1 cut(s) 651
BsrFI RCCGGY 1 cut(s) 272
BsrI ACTGG 1 cut(s) 72
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 3 cut(s) 731, 761, 866
BssMI GATC 2 cut(s) 618, 789
BssNI GRCGYC 3 cut(s) 282, 414, 603
Bst2UI CCWGG 4 cut(s) 515, 762, 858, 867
Bst4CI ACNGT 2 cut(s) 250, 349
Bst6I CTCTTC 2 cut(s) 584, 858
BstACI GRCGYC 3 cut(s) 282, 414, 603
BstBI TTCGAA 1 cut(s) 318
BstC8I GCNNGC 2 cut(s) 274, 575
BstDEI CTNAG 3 cut(s) 780, 894, 918
BstEII GGTNACC 1 cut(s) 590
BstF5I GGATG 1 cut(s) 438
BstFNI CGCG 1 cut(s) 444
BstHHI GCGC 1 cut(s) 494
BstKTI GATC 2 cut(s) 621, 792
BstMBI GATC 2 cut(s) 618, 789
BstMCI CGRYCG 2 cut(s) 448, 621
BstMWI GCNNNNNNNGC 3 cut(s) 309, 435, 441
BstNI CCWGG 4 cut(s) 515, 762, 858, 867
BstPI GGTNACC 1 cut(s) 590
BstSCI CCNGG 6 cut(s) 513, 730, 731, 760, 856, 865
BstSFI CTRYAG 1 cut(s) 108
BstUI CGCG 1 cut(s) 444
BstV2I GAAGAC 2 cut(s) 68, 518
BsuRI GGCC 4 cut(s) 272, 290, 474, 719
BtsCI GGATG 1 cut(s) 438
Cac8I GCNNGC 2 cut(s) 274, 575
CfoI GCGC 1 cut(s) 494
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 4 cut(s) 472, 684, 717, 823
Cfr9I CCCGGG 1 cut(s) 731
CseI GACGC 2 cut(s) 271, 422
CviAII CATG 4 cut(s) 256, 286, 304, 680
DdeI CTNAG 3 cut(s) 780, 894, 918
DpnI GATC 2 cut(s) 620, 791
DpnII GATC 2 cut(s) 618, 789
DrdI GACNNNNNNGTC 1 cut(s) 682
DseDI GACNNNNNNGTC 1 cut(s) 682
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 2 cut(s) 584, 858
EarI CTCTTC 2 cut(s) 584, 858
EciI GGCGGA 1 cut(s) 494
Ecl136II GAGCTC 1 cut(s) 577
Eco24I GRGCYC 2 cut(s) 552, 579
Eco47I GGWCC 2 cut(s) 684, 823
Eco53kI GAGCTC 1 cut(s) 577
Eco57I CTGAAG 1 cut(s) 512
Eco88I CYCGRG 1 cut(s) 731
Eco91I GGTNACC 1 cut(s) 590
EcoICRI GAGCTC 1 cut(s) 577
EcoO109I RGGNCCY 1 cut(s) 823
EcoO65I GGTNACC 1 cut(s) 590
EcoRII CCWGG 4 cut(s) 513, 760, 856, 865
EcoT38I GRGCYC 2 cut(s) 552, 579
FaeI CATG 4 cut(s) 259, 289, 307, 683
FaiI YATR 7 cut(s) 60, 110, 257, 287, 305, 681, 839
FaqI GGGAC 2 cut(s) 323, 790
FatI CATG 4 cut(s) 255, 285, 303, 679
FblI GTMKAC 1 cut(s) 600
Fnu4HI GCNGC 1 cut(s) 436
FokI GGATG 1 cut(s) 425
FriOI GRGCYC 2 cut(s) 552, 579
Fsp4HI GCNGC 1 cut(s) 436
GlaI GCGC 1 cut(s) 493
GluI GCNGC 1 cut(s) 436
GsuI CTGGAG 1 cut(s) 497
HaeIII GGCC 4 cut(s) 272, 290, 474, 719
HapII CCGG 7 cut(s) 273, 448, 489, 609, 720, 732, 890
HgaI GACGC 2 cut(s) 271, 422
HhaI GCGC 1 cut(s) 494
Hin1I GRCGYC 3 cut(s) 282, 414, 603
Hin1II CATG 4 cut(s) 259, 289, 307, 683
Hin6I GCGC 1 cut(s) 492
HinP1I GCGC 1 cut(s) 492
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 2 cut(s) 676, 691
HpaII CCGG 7 cut(s) 273, 448, 489, 609, 720, 732, 890
HphI GGTGA 6 cut(s) 211, 244, 337, 370, 448, 602
Hpy166II GTNNAC 2 cut(s) 601, 742
Hpy188I TCNGA 1 cut(s) 94
Hpy188III TCNNGA 6 cut(s) 530, 622, 661, 793, 802, 811
Hpy8I GTNNAC 2 cut(s) 601, 742
Hpy99I CGWCG 7 cut(s) 353, 356, 407, 416, 602, 605, 608
HpyAV CCTTC 4 cut(s) 173, 359, 533, 884
HpyCH4III ACNGT 2 cut(s) 250, 349
HpyCH4IV ACGT 1 cut(s) 603
HpyF10VI GCNNNNNNNGC 3 cut(s) 309, 435, 441
HpyF3I CTNAG 3 cut(s) 780, 894, 918
HpySE526I ACGT 1 cut(s) 603
Hsp92I GRCGYC 3 cut(s) 282, 414, 603
Hsp92II CATG 4 cut(s) 259, 289, 307, 683
HspAI GCGC 1 cut(s) 492
KroI GCCGGC 1 cut(s) 272
KroNI GCCGGC 1 cut(s) 274
Kzo9I GATC 2 cut(s) 618, 789
LguI GCTCTTC 2 cut(s) 584, 858
LweI GCATC 3 cut(s) 285, 447, 747
MaeII ACGT 1 cut(s) 603
MaeIII GTNAC 5 cut(s) 250, 343, 376, 590, 692
MalI GATC 2 cut(s) 620, 791
MboI GATC 2 cut(s) 618, 789
MhlI GDGCHC 4 cut(s) 105, 552, 579, 853
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 2 cut(s) 52, 147
MluNI TGGCCA 1 cut(s) 290
MlyI GAGTC 2 cut(s) 670, 700
Mox20I TGGCCA 1 cut(s) 290
MroNI GCCGGC 1 cut(s) 272
MscI TGGCCA 1 cut(s) 290
MseI TTAA 3 cut(s) 645, 843, 925
MslI CAYNNNNRTG 1 cut(s) 257
Msp20I TGGCCA 1 cut(s) 290
MspI CCGG 7 cut(s) 273, 448, 489, 609, 720, 732, 890
MspR9I CCNGG 6 cut(s) 515, 732, 733, 762, 858, 867
MvaI CCWGG 4 cut(s) 515, 762, 858, 867
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 3 cut(s) 309, 435, 441
NaeI GCCGGC 1 cut(s) 274
NciI CCSGG 2 cut(s) 732, 733
NdeII GATC 2 cut(s) 618, 789
NgoMIV GCCGGC 1 cut(s) 272
NlaIII CATG 4 cut(s) 259, 289, 307, 683
NlaIV GGNNCC 6 cut(s) 613, 653, 685, 718, 724, 872
NmeAIII GCCGAG 1 cut(s) 279
NmuCI GTSAC 5 cut(s) 250, 343, 376, 590, 692
NspV TTCGAA 1 cut(s) 318
PciSI GCTCTTC 2 cut(s) 584, 858
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PdiI GCCGGC 1 cut(s) 274
PflFI GACNNNGTC 1 cut(s) 596
PfoI TCCNGGA 1 cut(s) 513
PkrI GCNGC 1 cut(s) 437
Ple19I CGATCG 1 cut(s) 621
PleI GAGTC 2 cut(s) 670, 699
PpsI GAGTC 2 cut(s) 670, 699
PpuMI RGGWCCY 1 cut(s) 823
PshBI ATTAAT 1 cut(s) 843
Psp124BI GAGCTC 1 cut(s) 579
Psp5II RGGWCCY 1 cut(s) 823
Psp6I CCWGG 4 cut(s) 513, 760, 856, 865
PspEI GGTNACC 1 cut(s) 590
PspGI CCWGG 4 cut(s) 513, 760, 856, 865
PspN4I GGNNCC 6 cut(s) 613, 653, 685, 718, 724, 872
PspPI GGNCC 4 cut(s) 472, 684, 717, 823
PspPPI RGGWCCY 1 cut(s) 823
PsyI GACNNNGTC 1 cut(s) 596
PvuI CGATCG 1 cut(s) 621
RseI CAYNNNNRTG 1 cut(s) 257
SacI GAGCTC 1 cut(s) 579
SalI GTCGAC 1 cut(s) 599
SapI GCTCTTC 2 cut(s) 584, 858
SaqAI TTAA 3 cut(s) 645, 843, 925
SatI GCNGC 1 cut(s) 436
Sau3AI GATC 2 cut(s) 618, 789
Sau96I GGNCC 4 cut(s) 472, 684, 717, 823
SchI GAGTC 2 cut(s) 670, 700
ScrFI CCNGG 6 cut(s) 515, 732, 733, 762, 858, 867
SduI GDGCHC 4 cut(s) 105, 552, 579, 853
SfaNI GCATC 3 cut(s) 285, 447, 747
SfcI CTRYAG 1 cut(s) 108
SfuI TTCGAA 1 cut(s) 318
SgrDI CGTCGACG 1 cut(s) 599
SinI GGWCC 2 cut(s) 684, 823
SmaI CCCGGG 1 cut(s) 733
SmiMI CAYNNNNRTG 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 800
SmoI CTYRAG 1 cut(s) 800
Sse9I AATT 2 cut(s) 52, 147
SsiI CCGC 5 cut(s) 433, 436, 442, 505, 880
SstI GAGCTC 1 cut(s) 579
StyD4I CCNGG 6 cut(s) 513, 730, 731, 760, 856, 865
TaaI ACNGT 2 cut(s) 250, 349
TaiI ACGT 1 cut(s) 606
TaqI TCGA 3 cut(s) 318, 600, 710
TasI AATT 2 cut(s) 52, 147
TauI GCSGC 1 cut(s) 438
Tru1I TTAA 3 cut(s) 645, 843, 925
Tru9I TTAA 3 cut(s) 645, 843, 925
TseFI GTSAC 5 cut(s) 250, 343, 376, 590, 692
Tsp45I GTSAC 5 cut(s) 250, 343, 376, 590, 692
TspMI CCCGGG 1 cut(s) 731
Tth111I GACNNNGTC 1 cut(s) 596
VpaK11BI GGWCC 2 cut(s) 684, 823
VspI ATTAAT 1 cut(s) 843
XcmI CCANNNNNNNNNTGG 1 cut(s) 466
XmaI CCCGGG 1 cut(s) 731
XmiI GTMKAC 1 cut(s) 600
ZraI GACGTC 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.