pycom07g06550

Belongs to the universal ribosomal protein uL3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0015186
Physical Location & Seq
Forward (+)
5851290 .. 5852039
750 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g06550.1

Sequence Viewer

Length: 750 bp
ATGTCAATCCTTTCCATTTCTTCACCTTCTTCTTCCTCCGCTCTCCTCTCCAATGTCTCCTCCTCCAAATCCTCCCCATTCATCACCCCGCCACTCCAAATCGCCGTCGCAAAATCCCACAAAAAGCGACAGACGGGAGTCACCATGAGCATGGAGGCCGGCATCGGTGTCATGGCCACGAAGCTCGGCATGATGAGCTTCTTCGAGCCCACCGGCAAAGTCGTCCCGGTTACCGTCGTGGGTTTCAAGGAGGGCAACATCGTCACCCAGGTAAAAACCCAGGCCACCGACGGCTACGACGCCGTCCAAATCGGCTACCGGCGGGTCCGTGACCGGAAGCTGACGAAGCCCGAAATGGGCCACCTCCAGAAAGCCGGCTCGATCCCCCTCCGCCACCTCCAGGAGTTCCGGCTGCAAGACGTCGAAGGATTCGAGCCGAACCAGAAGCTCTCCTTCGACGAGCTTTTCAAAGAGGGTGACATCGTCGACGTCTCCGGAACCACAATCGGAAAGGGGTTTCAGGGAGGAATCAAGCGGCACAATTTCAAGCGGGGGCTGATGACCCACGGATCCAAGAGCCACAGAGCTCTGGGTTCAATCGGTGCCGGAACGACGCCTGGAAGGGTGTACAAGGGGAAGAAGATGCCGGGGAGAATGGGAGGGACCAAGACCAAGATCAGGAAGCTGAAGATTGTGAAGATCGATAAGGAGCTCAATGTTGTGATCGATCAAAGGCGCCCTGCCCGGTAA

Protein Analysis

250

Amino Acids

27.12

Weight (kDa)

10.46

Isoelectric Point (pI)

41.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 149 - 236 5.7e-15 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43030
fragaria_vesca FvH4_7g08200
malus_domestica MD02G1230700.v1.1 MD07G1082200.v1.1
prunus_persica Prupe.2G107400_v2.0.a1
pyrus_communis pycom02g19980 pycom07g06550
rosa_chinensis RchiOBHm_Chr1g0340951
rosa_laevigata RLG00000029053
rosa_multiflora Rmu_sc0002200.1_g000047
rosa_roxburghii Rroxscaffold_4G00311600
rosa_rugosa Rorug01G0160600.1
rosa_samantha Rh1AG175300 Rh1BG143600 Rh1DG175200
rosa_wichuraiana Rw1G014690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 423, 492
AccB1I GGYRCC 2 cut(s) 602, 735
AccBSI CCGCTC 1 cut(s) 41
AccI GTMKAC 1 cut(s) 486
AccIII TCCGGA 1 cut(s) 494
AciI CCGC 6 cut(s) 39, 89, 322, 391, 535, 550
AclWI GGATC 3 cut(s) 376, 564, 577
AcoI YGGCCR 1 cut(s) 174
AcuI CTGAAG 1 cut(s) 707
AcyI GRCGYC 5 cut(s) 300, 420, 489, 614, 736
AfaI GTAC 1 cut(s) 629
AfiI CCNNNNNNNGG 3 cut(s) 356, 400, 678
AgsI TTSAA 4 cut(s) 247, 469, 547, 597
AjnI CCWGG 4 cut(s) 267, 279, 399, 616
AluBI AGCT 8 cut(s) 184, 198, 340, 448, 463, 587, 685, 712
AluI AGCT 8 cut(s) 184, 198, 340, 448, 463, 587, 685, 712
Alw21I GWGCWC 2 cut(s) 589, 714
Alw26I GTCTC 2 cut(s) 61, 496
AlwI GGATC 3 cut(s) 376, 564, 577
Aor13HI TCCGGA 1 cut(s) 494
AoxI GGCC 4 cut(s) 156, 174, 282, 358
ApeKI GCWGC 1 cut(s) 412
AspLEI GCGC 1 cut(s) 738
AspS9I GGNCC 3 cut(s) 325, 358, 663
AsuC2I CCSGG 3 cut(s) 227, 648, 745
AsuHPI GGTGA 5 cut(s) 15, 76, 133, 256, 488
AvaII GGWCC 2 cut(s) 325, 663
BalI TGGCCA 1 cut(s) 176
BamHI GGATCC 1 cut(s) 569
BanI GGYRCC 2 cut(s) 602, 735
BanII GRGCYC 3 cut(s) 210, 589, 714
Bbv12I GWGCWC 2 cut(s) 589, 714
BbvI GCAGC 1 cut(s) 399
BceAI ACGGC 3 cut(s) 89, 287, 307
BciT130I CCWGG 4 cut(s) 269, 281, 401, 618
BcnI CCSGG 3 cut(s) 227, 648, 745
BcoDI GTCTC 2 cut(s) 61, 496
BfoI RGCGCY 1 cut(s) 739
BisI GCNGC 2 cut(s) 413, 536
BlsI GCNGC 2 cut(s) 414, 537
Bme1390I CCNGG 7 cut(s) 227, 269, 281, 401, 618, 648, 745
Bme18I GGWCC 2 cut(s) 325, 663
BmgT120I GGNCC 3 cut(s) 325, 358, 663
BmiI GGNNCC 6 cut(s) 326, 499, 571, 604, 664, 737
BmrFI CCNGG 7 cut(s) 227, 269, 281, 401, 618, 648, 745
BmsI GCATC 2 cut(s) 171, 633
BoxI GACNNNNGTC 1 cut(s) 137
BpmI CTGGAG 2 cut(s) 350, 383
BpuMI CCSGG 3 cut(s) 227, 648, 745
Bsa29I ATCGAT 2 cut(s) 702, 726
BsaHI GRCGYC 5 cut(s) 300, 420, 489, 614, 736
BsaJI CCNNGG 4 cut(s) 267, 279, 565, 647
BsaWI WCCGGW 2 cut(s) 333, 494
Bsc4I CCNNNNNNNGG 3 cut(s) 356, 400, 678
Bse118I RCCGGY 4 cut(s) 158, 212, 318, 374
BseAI TCCGGA 1 cut(s) 494
BseBI CCWGG 4 cut(s) 269, 281, 401, 618
BseCI ATCGAT 2 cut(s) 702, 726
BseDI CCNNGG 4 cut(s) 267, 279, 565, 647
BseLI CCNNNNNNNGG 3 cut(s) 356, 400, 678
BseRI GAGGAG 3 cut(s) 35, 49, 52
BseXI GCAGC 1 cut(s) 399
BshFI GGCC 4 cut(s) 158, 176, 284, 360
BshNI GGYRCC 2 cut(s) 602, 735
BshVI ATCGAT 2 cut(s) 702, 726
BsiHKAI GWGCWC 2 cut(s) 589, 714
BslFI GGGAC 2 cut(s) 209, 676
BslI CCNNNNNNNGG 3 cut(s) 356, 400, 678
BsmAI GTCTC 2 cut(s) 61, 496
BsmBI CGTCTC 1 cut(s) 496
BsmFI GGGAC 2 cut(s) 209, 676
BsnI GGCC 4 cut(s) 158, 176, 284, 360
Bsp1286I GDGCHC 3 cut(s) 210, 589, 714
Bsp13I TCCGGA 1 cut(s) 494
Bsp1407I TGTACA 1 cut(s) 627
Bsp143I GATC 6 cut(s) 381, 569, 675, 699, 723, 727
BspACI CCGC 6 cut(s) 39, 89, 322, 391, 535, 550
BspANI GGCC 4 cut(s) 158, 176, 284, 360
BspDI ATCGAT 2 cut(s) 702, 726
BspEI TCCGGA 1 cut(s) 494
BspLI GGNNCC 6 cut(s) 326, 499, 571, 604, 664, 737
BspPI GGATC 3 cut(s) 376, 564, 577
BspT107I GGYRCC 2 cut(s) 602, 735
BsrBI CCGCTC 1 cut(s) 41
BsrFI RCCGGY 4 cut(s) 158, 212, 318, 374
BsrGI TGTACA 1 cut(s) 627
BssAI RCCGGY 4 cut(s) 158, 212, 318, 374
BssECI CCNNGG 4 cut(s) 267, 279, 565, 647
BssMI GATC 6 cut(s) 381, 569, 675, 699, 723, 727
BssNI GRCGYC 5 cut(s) 300, 420, 489, 614, 736
Bst2UI CCWGG 4 cut(s) 269, 281, 401, 618
Bst4CI ACNGT 1 cut(s) 235
BstACI GRCGYC 5 cut(s) 300, 420, 489, 614, 736
BstAUI TGTACA 1 cut(s) 627
BstC8I GCNNGC 2 cut(s) 160, 376
BstDSI CCRYGG 1 cut(s) 565
BstEII GGTNACC 1 cut(s) 229
BstH2I RGCGCY 1 cut(s) 739
BstHHI GCGC 1 cut(s) 738
BstKTI GATC 6 cut(s) 384, 572, 678, 702, 726, 730
BstMAI GTCTC 2 cut(s) 61, 496
BstMBI GATC 6 cut(s) 381, 569, 675, 699, 723, 727
BstMWI GCNNNNNNNGC 2 cut(s) 195, 346
BstNI CCWGG 4 cut(s) 269, 281, 401, 618
BstPAI GACNNNNGTC 1 cut(s) 137
BstPI GGTNACC 1 cut(s) 229
BstSCI CCNGG 7 cut(s) 225, 267, 279, 399, 616, 646, 743
BstV1I GCAGC 1 cut(s) 399
BstX2I RGATCY 1 cut(s) 569
BstXI CCANNNNNNTGG 1 cut(s) 151
BstYI RGATCY 1 cut(s) 569
Bsu15I ATCGAT 2 cut(s) 702, 726
BsuRI GGCC 4 cut(s) 158, 176, 284, 360
BsuTUI ATCGAT 2 cut(s) 702, 726
BtgI CCRYGG 1 cut(s) 565
Cac8I GCNNGC 2 cut(s) 160, 376
CfoI GCGC 1 cut(s) 738
Cfr10I RCCGGY 4 cut(s) 158, 212, 318, 374
Cfr13I GGNCC 3 cut(s) 325, 358, 663
ClaI ATCGAT 2 cut(s) 702, 726
CseI GACGC 2 cut(s) 308, 622
Csp6I GTAC 1 cut(s) 628
CviAII CATG 4 cut(s) 145, 151, 172, 190
CviQI GTAC 1 cut(s) 628
DinI GGCGCC 1 cut(s) 737
DpnI GATC 6 cut(s) 383, 571, 677, 701, 725, 729
DpnII GATC 6 cut(s) 381, 569, 675, 699, 723, 727
EaeI YGGCCR 1 cut(s) 174
EciI GGCGGA 1 cut(s) 380
Ecl136II GAGCTC 2 cut(s) 587, 712
Eco24I GRGCYC 3 cut(s) 210, 589, 714
Eco47I GGWCC 2 cut(s) 325, 663
Eco53kI GAGCTC 2 cut(s) 587, 712
Eco57I CTGAAG 1 cut(s) 707
Eco91I GGTNACC 1 cut(s) 229
EcoICRI GAGCTC 2 cut(s) 587, 712
EcoO65I GGTNACC 1 cut(s) 229
EcoRII CCWGG 4 cut(s) 267, 279, 399, 616
EcoT38I GRGCYC 3 cut(s) 210, 589, 714
EgeI GGCGCC 1 cut(s) 737
EheI GGCGCC 1 cut(s) 737
Esp3I CGTCTC 1 cut(s) 496
FaeI CATG 4 cut(s) 148, 154, 175, 193
FaiI YATR 4 cut(s) 146, 152, 173, 191
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FaqI GGGAC 2 cut(s) 209, 676
FatI CATG 4 cut(s) 144, 150, 171, 189
FauI CCCGC 3 cut(s) 96, 315, 543
FblI GTMKAC 1 cut(s) 486
Fnu4HI GCNGC 2 cut(s) 413, 536
FriOI GRGCYC 3 cut(s) 210, 589, 714
Fsp4HI GCNGC 2 cut(s) 413, 536
GlaI GCGC 1 cut(s) 737
GluI GCNGC 2 cut(s) 413, 536
GsuI CTGGAG 2 cut(s) 350, 383
HaeII RGCGCY 1 cut(s) 739
HaeIII GGCC 4 cut(s) 158, 176, 284, 360
HgaI GACGC 2 cut(s) 308, 622
HhaI GCGC 1 cut(s) 738
Hin1I GRCGYC 5 cut(s) 300, 420, 489, 614, 736
Hin1II CATG 4 cut(s) 148, 154, 175, 193
Hin6I GCGC 1 cut(s) 736
HinP1I GCGC 1 cut(s) 736
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HinfI GANTC 3 cut(s) 138, 429, 528
HphI GGTGA 5 cut(s) 15, 76, 133, 256, 488
Hpy166II GTNNAC 2 cut(s) 487, 628
Hpy188I TCNGA 1 cut(s) 509
Hpy188III TCNNGA 3 cut(s) 367, 495, 679
Hpy8I GTNNAC 2 cut(s) 487, 628
Hpy99I CGWCG 9 cut(s) 110, 239, 293, 302, 425, 461, 488, 491, 616
HpyAV CCTTC 4 cut(s) 36, 419, 463, 615
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4IV ACGT 2 cut(s) 420, 489
HpyCH4V TGCA 1 cut(s) 415
HpyF10VI GCNNNNNNNGC 2 cut(s) 195, 346
HpySE526I ACGT 2 cut(s) 420, 489
Hsp92I GRCGYC 5 cut(s) 300, 420, 489, 614, 736
Hsp92II CATG 4 cut(s) 148, 154, 175, 193
HspAI GCGC 1 cut(s) 736
KasI GGCGCC 1 cut(s) 735
Kpn2I TCCGGA 1 cut(s) 494
KroI GCCGGC 2 cut(s) 158, 374
KroNI GCCGGC 2 cut(s) 160, 376
Kzo9I GATC 6 cut(s) 381, 569, 675, 699, 723, 727
LmnI GCTCC 1 cut(s) 709
Lsp1109I GCAGC 1 cut(s) 399
LweI GCATC 2 cut(s) 171, 633
MaeII ACGT 2 cut(s) 420, 489
MaeIII GTNAC 5 cut(s) 139, 229, 262, 329, 476
MalI GATC 6 cut(s) 383, 571, 677, 701, 725, 729
MbiI CCGCTC 1 cut(s) 41
MboI GATC 6 cut(s) 381, 569, 675, 699, 723, 727
MboII GAAGA 8 cut(s) 12, 21, 24, 193, 649, 652, 700, 709
MflI RGATCY 1 cut(s) 569
MhlI GDGCHC 3 cut(s) 210, 589, 714
MlsI TGGCCA 1 cut(s) 176
MluCI AATT 1 cut(s) 541
MluNI TGGCCA 1 cut(s) 176
Mly113I GGCGCC 1 cut(s) 736
MlyI GAGTC 1 cut(s) 147
Mox20I TGGCCA 1 cut(s) 176
MroI TCCGGA 1 cut(s) 494
MroNI GCCGGC 2 cut(s) 158, 374
MscI TGGCCA 1 cut(s) 176
MslI CAYNNNNRTG 1 cut(s) 149
Msp20I TGGCCA 1 cut(s) 176
MspR9I CCNGG 7 cut(s) 227, 269, 281, 401, 618, 648, 745
MvaI CCWGG 4 cut(s) 269, 281, 401, 618
MwoI GCNNNNNNNGC 2 cut(s) 195, 346
NaeI GCCGGC 2 cut(s) 160, 376
NarI GGCGCC 1 cut(s) 736
NciI CCSGG 3 cut(s) 227, 648, 745
NdeII GATC 6 cut(s) 381, 569, 675, 699, 723, 727
NgoMIV GCCGGC 2 cut(s) 158, 374
NlaIII CATG 4 cut(s) 148, 154, 175, 193
NlaIV GGNNCC 6 cut(s) 326, 499, 571, 604, 664, 737
NmeAIII GCCGAG 1 cut(s) 165
NmuCI GTSAC 4 cut(s) 139, 262, 329, 476
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PdiI GCCGGC 2 cut(s) 160, 376
PfeI GAWTC 2 cut(s) 429, 528
PflFI GACNNNGTC 2 cut(s) 302, 482
PfoI TCCNGGA 1 cut(s) 399
PkrI GCNGC 2 cut(s) 414, 537
PleI GAGTC 1 cut(s) 146
PluTI GGCGCC 1 cut(s) 739
PpsI GAGTC 1 cut(s) 146
PshAI GACNNNNGTC 1 cut(s) 137
Psp124BI GAGCTC 2 cut(s) 589, 714
Psp6I CCWGG 4 cut(s) 267, 279, 399, 616
PspEI GGTNACC 1 cut(s) 229
PspGI CCWGG 4 cut(s) 267, 279, 399, 616
PspN4I GGNNCC 6 cut(s) 326, 499, 571, 604, 664, 737
PspPI GGNCC 3 cut(s) 325, 358, 663
PsuI RGATCY 1 cut(s) 569
PsyI GACNNNGTC 2 cut(s) 302, 482
RsaI GTAC 1 cut(s) 629
RsaNI GTAC 1 cut(s) 628
RseI CAYNNNNRTG 1 cut(s) 149
SacI GAGCTC 2 cut(s) 589, 714
SalI GTCGAC 1 cut(s) 485
SatI GCNGC 2 cut(s) 413, 536
Sau3AI GATC 6 cut(s) 381, 569, 675, 699, 723, 727
Sau96I GGNCC 3 cut(s) 325, 358, 663
SchI GAGTC 1 cut(s) 147
ScrFI CCNGG 7 cut(s) 227, 269, 281, 401, 618, 648, 745
SduI GDGCHC 3 cut(s) 210, 589, 714
SfaNI GCATC 2 cut(s) 171, 633
SfoI GGCGCC 1 cut(s) 737
SgrDI CGTCGACG 1 cut(s) 485
SinI GGWCC 2 cut(s) 325, 663
SmiMI CAYNNNNRTG 1 cut(s) 149
Sse9I AATT 1 cut(s) 541
SsiI CCGC 6 cut(s) 39, 89, 322, 391, 535, 550
SspDI GGCGCC 1 cut(s) 735
SstI GAGCTC 2 cut(s) 589, 714
StyD4I CCNGG 7 cut(s) 225, 267, 279, 399, 616, 646, 743
TaaI ACNGT 1 cut(s) 235
TaiI ACGT 2 cut(s) 423, 492
TaqI TCGA 8 cut(s) 204, 380, 423, 432, 456, 486, 702, 726
TasI AATT 1 cut(s) 541
TatI WGTACW 1 cut(s) 627
TauI GCSGC 1 cut(s) 538
TfiI GAWTC 2 cut(s) 429, 528
TseFI GTSAC 4 cut(s) 139, 262, 329, 476
TseI GCWGC 1 cut(s) 412
Tsp45I GTSAC 4 cut(s) 139, 262, 329, 476
TspDTI ATGAA 1 cut(s) 70
TspGWI ACGGA 2 cut(s) 317, 582
Tth111I GACNNNGTC 2 cut(s) 302, 482
VpaK11BI GGWCC 2 cut(s) 325, 663
XmiI GTMKAC 1 cut(s) 486
ZraI GACGTC 2 cut(s) 421, 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.