Rh1DG175200

Belongs to the universal ribosomal protein uL3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0015186
Physical Location & Seq
Forward (+)
35924718 .. 35926240
1523 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG175200.1

Sequence Viewer

Length: 795 bp
ATGGCACTTCTTCCAATTCACAATGCTCTCCTTCCCAATCTCAAACCCTCTCCCTTTCTCACACCCAAAAACCCATTCCCCCTCCTCACCAAAACCCACAAGAAGCCCCACTCCACAGTCACCATGATGAGCATGGAGGCCGGCATCGGCGTCATGGCCACCAAGCTCGGCATGATGAGCTTCTTCGAACCCAACGGCAAGGTCGTCCCCGTCACCGTCGTCGGCTTCAAAGAAGGCAACATTGTCACCCAAATCAAAACCCAAGCCACCGACGGCTACGACGCCGTCCAAGTCGGCTACCGCCGCATCCGCGACCGGAAACTCACCAAGCCCGAAATGGGTCATCTCAACAAGGCCGGCGCAATCCCTCTCCGCCACCTCCAGGAGTTTCGTCTTCAGGACGTTGAAGGGTTTGAGCCCAACCAGAAGCTTGATTTCAGCGAGCTCTTCAAGGAGGGTGACCTCGTCGACGTCGCCGGAACCACGATCGGAAAAGGGTTTCAGGGTGGGATTAAGAGGCACCATTTCAAGAGGGGGCTGATGACTCATGGGTCCAAGAGTCACAGACAGCTTGGGTCGATTGGGGCCGGAACCACGCCGGGGAGGGTTTACAAGGGGAAGAAGATGCCTGGGAGAATGGGAGGGACTAAGACCAAGATCAGGAAGCTCAAGATTGTCAAGATTGATAAGGACCTCAATGTGGTTATGATTAATGGTGCTCTTCCTGGTAAGCCTGGGAACCTTCTCCGCATTACTCCCGCTAAGATTGTAGGAAAGAATATCCCCAAGAGTTAA

Protein Analysis

264

Amino Acids

28.9

Weight (kDa)

10.42

Isoelectric Point (pI)

26.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 143 - 230 8.2e-16 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43030
fragaria_vesca FvH4_7g08200
malus_domestica MD02G1230700.v1.1 MD07G1082200.v1.1
prunus_persica Prupe.2G107400_v2.0.a1
pyrus_communis pycom02g19980 pycom07g06550
rosa_chinensis RchiOBHm_Chr1g0340951
rosa_laevigata RLG00000029053
rosa_multiflora Rmu_sc0002200.1_g000047
rosa_roxburghii Rroxscaffold_4G00311600
rosa_rugosa Rorug01G0160600.1
rosa_samantha Rh1AG175300 Rh1BG143600 Rh1DG175200
rosa_wichuraiana Rw1G014690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 550
AatII GACGTC 1 cut(s) 474
AccB1I GGYRCC 1 cut(s) 519
AccI GTMKAC 1 cut(s) 468
AccII CGCG 1 cut(s) 312
AciI CCGC 6 cut(s) 301, 304, 310, 373, 748, 759
AcoI YGGCCR 1 cut(s) 156
AcuI CTGAAG 1 cut(s) 380
AcyI GRCGYC 3 cut(s) 150, 282, 471
AfiI CCNNNNNNNGG 5 cut(s) 338, 382, 600, 660, 700
AgsI TTSAA 4 cut(s) 229, 407, 451, 529
AjnI CCWGG 4 cut(s) 381, 628, 724, 733
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AluBI AGCT 6 cut(s) 166, 180, 430, 445, 571, 667
AluI AGCT 6 cut(s) 166, 180, 430, 445, 571, 667
Alw21I GWGCWC 2 cut(s) 447, 721
AoxI GGCC 4 cut(s) 138, 156, 354, 585
AseI ATTAAT 1 cut(s) 711
AspLEI GCGC 1 cut(s) 362
AspS9I GGNCC 3 cut(s) 552, 585, 691
AsuC2I CCSGG 1 cut(s) 600
AsuHPI GGTGA 6 cut(s) 79, 112, 205, 238, 316, 470
AsuII TTCGAA 1 cut(s) 186
AvaII GGWCC 2 cut(s) 552, 691
BalI TGGCCA 1 cut(s) 158
BanI GGYRCC 1 cut(s) 519
BanII GRGCYC 2 cut(s) 420, 447
BbsI GAAGAC 1 cut(s) 386
Bbv12I GWGCWC 2 cut(s) 447, 721
BceAI ACGGC 3 cut(s) 211, 269, 289
BciT130I CCWGG 4 cut(s) 383, 630, 726, 735
BcnI CCSGG 1 cut(s) 600
BisI GCNGC 1 cut(s) 304
BlsI GCNGC 1 cut(s) 305
Bme1390I CCNGG 5 cut(s) 383, 600, 630, 726, 735
Bme18I GGWCC 2 cut(s) 552, 691
BmgT120I GGNCC 3 cut(s) 552, 585, 691
BmiI GGNNCC 6 cut(s) 481, 521, 553, 586, 592, 740
BmrFI CCNGG 5 cut(s) 383, 600, 630, 726, 735
BmsI GCATC 3 cut(s) 153, 315, 615
BpiI GAAGAC 1 cut(s) 386
BpmI CTGGAG 1 cut(s) 365
Bpu14I TTCGAA 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 653
BpuMI CCSGG 1 cut(s) 600
BsaHI GRCGYC 3 cut(s) 150, 282, 471
BsaJI CCNNGG 3 cut(s) 599, 629, 734
BsaWI WCCGGW 1 cut(s) 315
Bsc4I CCNNNNNNNGG 5 cut(s) 338, 382, 600, 660, 700
Bse118I RCCGGY 2 cut(s) 140, 356
BseBI CCWGG 4 cut(s) 383, 630, 726, 735
BseDI CCNNGG 3 cut(s) 599, 629, 734
BseGI GGATG 1 cut(s) 306
BseLI CCNNNNNNNGG 5 cut(s) 338, 382, 600, 660, 700
BseRI GAGGAG 1 cut(s) 74
Bsh1236I CGCG 1 cut(s) 312
Bsh1285I CGRYCG 2 cut(s) 316, 489
BshFI GGCC 4 cut(s) 140, 158, 356, 587
BshNI GGYRCC 1 cut(s) 519
BsiEI CGRYCG 2 cut(s) 316, 489
BsiHKAI GWGCWC 2 cut(s) 447, 721
BsiSI CCGG 6 cut(s) 141, 316, 357, 477, 588, 599
BslFI GGGAC 2 cut(s) 191, 658
BslI CCNNNNNNNGG 5 cut(s) 338, 382, 600, 660, 700
BsmFI GGGAC 2 cut(s) 191, 658
BsnI GGCC 4 cut(s) 140, 158, 356, 587
Bsp119I TTCGAA 1 cut(s) 186
Bsp1286I GDGCHC 3 cut(s) 420, 447, 721
Bsp143I GATC 2 cut(s) 486, 657
BspACI CCGC 6 cut(s) 301, 304, 310, 373, 748, 759
BspANI GGCC 4 cut(s) 140, 158, 356, 587
BspFNI CGCG 1 cut(s) 312
BspLI GGNNCC 6 cut(s) 481, 521, 553, 586, 592, 740
BspQI GCTCTTC 2 cut(s) 452, 726
BspT104I TTCGAA 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 519
BsrFI RCCGGY 2 cut(s) 140, 356
BssAI RCCGGY 2 cut(s) 140, 356
BssECI CCNNGG 3 cut(s) 599, 629, 734
BssMI GATC 2 cut(s) 486, 657
BssNI GRCGYC 3 cut(s) 150, 282, 471
Bst2UI CCWGG 4 cut(s) 383, 630, 726, 735
Bst4CI ACNGT 2 cut(s) 118, 217
Bst6I CTCTTC 2 cut(s) 452, 726
BstACI GRCGYC 3 cut(s) 150, 282, 471
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 3 cut(s) 142, 358, 443
BstDEI CTNAG 2 cut(s) 648, 762
BstEII GGTNACC 1 cut(s) 458
BstF5I GGATG 1 cut(s) 306
BstFNI CGCG 1 cut(s) 312
BstHHI GCGC 1 cut(s) 362
BstKTI GATC 2 cut(s) 489, 660
BstMBI GATC 2 cut(s) 486, 657
BstMCI CGRYCG 2 cut(s) 316, 489
BstMWI GCNNNNNNNGC 3 cut(s) 177, 303, 309
BstNI CCWGG 4 cut(s) 383, 630, 726, 735
BstPI GGTNACC 1 cut(s) 458
BstSCI CCNGG 5 cut(s) 381, 598, 628, 724, 733
BstUI CGCG 1 cut(s) 312
BstV2I GAAGAC 1 cut(s) 386
BsuRI GGCC 4 cut(s) 140, 158, 356, 587
BtsCI GGATG 1 cut(s) 306
Cac8I GCNNGC 3 cut(s) 142, 358, 443
CfoI GCGC 1 cut(s) 362
Cfr10I RCCGGY 2 cut(s) 140, 356
Cfr13I GGNCC 3 cut(s) 552, 585, 691
CseI GACGC 2 cut(s) 139, 290
CviAII CATG 5 cut(s) 124, 133, 154, 172, 548
DdeI CTNAG 2 cut(s) 648, 762
DpnI GATC 2 cut(s) 488, 659
DpnII GATC 2 cut(s) 486, 657
DrdI GACNNNNNNGTC 1 cut(s) 550
DseDI GACNNNNNNGTC 1 cut(s) 550
EaeI YGGCCR 1 cut(s) 156
Eam1104I CTCTTC 2 cut(s) 452, 726
EarI CTCTTC 2 cut(s) 452, 726
EciI GGCGGA 1 cut(s) 362
Ecl136II GAGCTC 1 cut(s) 445
Eco24I GRGCYC 2 cut(s) 420, 447
Eco47I GGWCC 2 cut(s) 552, 691
Eco53kI GAGCTC 1 cut(s) 445
Eco57I CTGAAG 1 cut(s) 380
Eco91I GGTNACC 1 cut(s) 458
EcoICRI GAGCTC 1 cut(s) 445
EcoO109I RGGNCCY 1 cut(s) 691
EcoO65I GGTNACC 1 cut(s) 458
EcoRII CCWGG 4 cut(s) 381, 628, 724, 733
EcoT38I GRGCYC 2 cut(s) 420, 447
FaeI CATG 5 cut(s) 127, 136, 157, 175, 551
FaiI YATR 6 cut(s) 125, 134, 155, 173, 549, 707
FaqI GGGAC 2 cut(s) 191, 658
FatI CATG 5 cut(s) 123, 132, 153, 171, 547
FauI CCCGC 1 cut(s) 766
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 1 cut(s) 304
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 2 cut(s) 420, 447
Fsp4HI GCNGC 1 cut(s) 304
GlaI GCGC 1 cut(s) 361
GluI GCNGC 1 cut(s) 304
GsuI CTGGAG 1 cut(s) 365
HaeIII GGCC 4 cut(s) 140, 158, 356, 587
HapII CCGG 6 cut(s) 141, 316, 357, 477, 588, 599
HgaI GACGC 2 cut(s) 139, 290
HhaI GCGC 1 cut(s) 362
Hin1I GRCGYC 3 cut(s) 150, 282, 471
Hin1II CATG 5 cut(s) 127, 136, 157, 175, 551
Hin6I GCGC 1 cut(s) 360
HinP1I GCGC 1 cut(s) 360
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HindIII AAGCTT 1 cut(s) 428
HinfI GANTC 2 cut(s) 544, 559
HpaII CCGG 6 cut(s) 141, 316, 357, 477, 588, 599
HphI GGTGA 6 cut(s) 79, 112, 205, 238, 316, 470
Hpy166II GTNNAC 2 cut(s) 469, 610
Hpy188I TCNGA 1 cut(s) 491
Hpy188III TCNNGA 5 cut(s) 398, 529, 661, 670, 679
Hpy8I GTNNAC 2 cut(s) 469, 610
Hpy99I CGWCG 7 cut(s) 221, 224, 275, 284, 470, 473, 476
HpyAV CCTTC 4 cut(s) 41, 227, 401, 752
HpyCH4III ACNGT 2 cut(s) 118, 217
HpyCH4IV ACGT 2 cut(s) 402, 471
HpyF10VI GCNNNNNNNGC 3 cut(s) 177, 303, 309
HpyF3I CTNAG 2 cut(s) 648, 762
HpySE526I ACGT 2 cut(s) 402, 471
Hsp92I GRCGYC 3 cut(s) 150, 282, 471
Hsp92II CATG 5 cut(s) 127, 136, 157, 175, 551
HspAI GCGC 1 cut(s) 360
KroI GCCGGC 2 cut(s) 140, 356
KroNI GCCGGC 2 cut(s) 142, 358
Kzo9I GATC 2 cut(s) 486, 657
LguI GCTCTTC 2 cut(s) 452, 726
LweI GCATC 3 cut(s) 153, 315, 615
MaeII ACGT 2 cut(s) 402, 471
MaeIII GTNAC 5 cut(s) 118, 211, 244, 458, 560
MalI GATC 2 cut(s) 488, 659
MboI GATC 2 cut(s) 486, 657
MboII GAAGA 6 cut(s) 175, 386, 439, 631, 634, 713
MhlI GDGCHC 3 cut(s) 420, 447, 721
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 1 cut(s) 15
MluNI TGGCCA 1 cut(s) 158
MlyI GAGTC 2 cut(s) 538, 568
Mox20I TGGCCA 1 cut(s) 158
MroNI GCCGGC 2 cut(s) 140, 356
MscI TGGCCA 1 cut(s) 158
MseI TTAA 3 cut(s) 513, 711, 793
MslI CAYNNNNRTG 1 cut(s) 125
Msp20I TGGCCA 1 cut(s) 158
MspI CCGG 6 cut(s) 141, 316, 357, 477, 588, 599
MspR9I CCNGG 5 cut(s) 383, 600, 630, 726, 735
MvaI CCWGG 4 cut(s) 383, 630, 726, 735
MvnI CGCG 1 cut(s) 312
MwoI GCNNNNNNNGC 3 cut(s) 177, 303, 309
NaeI GCCGGC 2 cut(s) 142, 358
NciI CCSGG 1 cut(s) 600
NdeII GATC 2 cut(s) 486, 657
NgoMIV GCCGGC 2 cut(s) 140, 356
NlaIII CATG 5 cut(s) 127, 136, 157, 175, 551
NlaIV GGNNCC 6 cut(s) 481, 521, 553, 586, 592, 740
NmeAIII GCCGAG 1 cut(s) 147
NmuCI GTSAC 5 cut(s) 118, 211, 244, 458, 560
NspV TTCGAA 1 cut(s) 186
PciSI GCTCTTC 2 cut(s) 452, 726
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PdiI GCCGGC 2 cut(s) 142, 358
PflFI GACNNNGTC 2 cut(s) 284, 464
PfoI TCCNGGA 1 cut(s) 381
PkrI GCNGC 1 cut(s) 305
Ple19I CGATCG 1 cut(s) 489
PleI GAGTC 2 cut(s) 538, 567
PpsI GAGTC 2 cut(s) 538, 567
PpuMI RGGWCCY 1 cut(s) 691
PshBI ATTAAT 1 cut(s) 711
Psp124BI GAGCTC 1 cut(s) 447
Psp5II RGGWCCY 1 cut(s) 691
Psp6I CCWGG 4 cut(s) 381, 628, 724, 733
PspEI GGTNACC 1 cut(s) 458
PspGI CCWGG 4 cut(s) 381, 628, 724, 733
PspN4I GGNNCC 6 cut(s) 481, 521, 553, 586, 592, 740
PspPI GGNCC 3 cut(s) 552, 585, 691
PspPPI RGGWCCY 1 cut(s) 691
PsyI GACNNNGTC 2 cut(s) 284, 464
PvuI CGATCG 1 cut(s) 489
RseI CAYNNNNRTG 1 cut(s) 125
SacI GAGCTC 1 cut(s) 447
SalI GTCGAC 1 cut(s) 467
SapI GCTCTTC 2 cut(s) 452, 726
SaqAI TTAA 3 cut(s) 513, 711, 793
SatI GCNGC 1 cut(s) 304
Sau3AI GATC 2 cut(s) 486, 657
Sau96I GGNCC 3 cut(s) 552, 585, 691
SchI GAGTC 2 cut(s) 538, 568
ScrFI CCNGG 5 cut(s) 383, 600, 630, 726, 735
SduI GDGCHC 3 cut(s) 420, 447, 721
SfaNI GCATC 3 cut(s) 153, 315, 615
SfuI TTCGAA 1 cut(s) 186
SgrDI CGTCGACG 1 cut(s) 467
SinI GGWCC 2 cut(s) 552, 691
SmiMI CAYNNNNRTG 1 cut(s) 125
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
Sse9I AATT 1 cut(s) 15
SsiI CCGC 6 cut(s) 301, 304, 310, 373, 748, 759
SstI GAGCTC 1 cut(s) 447
StyD4I CCNGG 5 cut(s) 381, 598, 628, 724, 733
TaaI ACNGT 2 cut(s) 118, 217
TaiI ACGT 2 cut(s) 405, 474
TaqI TCGA 3 cut(s) 186, 468, 578
TasI AATT 1 cut(s) 15
TauI GCSGC 1 cut(s) 306
Tru1I TTAA 3 cut(s) 513, 711, 793
Tru9I TTAA 3 cut(s) 513, 711, 793
TseFI GTSAC 5 cut(s) 118, 211, 244, 458, 560
Tsp45I GTSAC 5 cut(s) 118, 211, 244, 458, 560
Tth111I GACNNNGTC 2 cut(s) 284, 464
VpaK11BI GGWCC 2 cut(s) 552, 691
VspI ATTAAT 1 cut(s) 711
XcmI CCANNNNNNNNNTGG 2 cut(s) 130, 334
XmiI GTMKAC 1 cut(s) 468
ZraI GACGTC 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.