MD07G1082200.v1.1

Belongs to the universal ribosomal protein uL3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0015186
Physical Location & Seq
Forward (+)
8050320 .. 8053764
3445 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1082200.v1.1.491

Sequence Viewer

Length: 777 bp
ATGTCAATCCTTTCCATTTCTGTACCTTCTTCTTCCTCCGCTCTCCTCCCCAATGTCTCCTTCTCCTCCAAATCCTCCCCATTCCTCACCCCGCCACTCCAAATCGCCGTCGCAAAATCCCACAAAAAGCGACCGACGGGAGTCACCATGAGCATGGAGGCCGGCATCGGAGTCATGGCCACGAAGCTCGGCATGATGAGCTTCTTCGAGCCCACCGGAAAAGTCGTCCCGGTTACCGTCGTGGGTTTCAAGGAGGGCAACATCGTCACCCAGGTAAAAACCCAGGCCACCGACGGCTACGACGCCGTCCAAATCGGCTACCGGCGGGTCCGTGACCGGAAGCTGACGAAGCCCGAAATGGGTCACCTCCAGAAAGCCGGCTCGATCCCCCTCCGCCACCTCCAGGAGTTCCGGCTGCAAGACGTCGAAGGATTCGAGCCGAACCAGAAGCTCTCCTTCGACGAGCTTTTCAAAGAGGGCGACATCGTCGACGTCTCCGGAACCACAATCGGAAAGGGGTTTCAGGGAGGAATCAAGCGGCACAATTTCAAGCGGGGGCTGATGACCCACGGGTCCAAGAGCCACAGAGCTCTGGGTTCAATCGGTGCCGGAACGACGCCGGGAAGGGTGTACAAGGGGAAGAAGATGCCCGGGAGAATGGGAGGGACCAAGACCAAGATCAGGAAGCTGAAGATTGTGAAGATCGATAAGGAGCTCAATGTTGTGATGATCAAAGGTGCCCTGCCCGGAAGGGCAAGCTCTACTACTTGGAGCTGA

Protein Analysis

259

Amino Acids

27.91

Weight (kDa)

10.41

Isoelectric Point (pI)

35.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 150 - 237 1.8e-14 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43030
fragaria_vesca FvH4_7g08200
malus_domestica MD02G1230700.v1.1 MD07G1082200.v1.1
prunus_persica Prupe.2G107400_v2.0.a1
pyrus_communis pycom02g19980 pycom07g06550
rosa_chinensis RchiOBHm_Chr1g0340951
rosa_laevigata RLG00000029053
rosa_multiflora Rmu_sc0002200.1_g000047
rosa_roxburghii Rroxscaffold_4G00311600
rosa_rugosa Rorug01G0160600.1
rosa_samantha Rh1AG175300 Rh1BG143600 Rh1DG175200
rosa_wichuraiana Rw1G014690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 571
AatII GACGTC 2 cut(s) 426, 495
AccB1I GGYRCC 2 cut(s) 605, 737
AccBSI CCGCTC 1 cut(s) 41
AccI GTMKAC 1 cut(s) 489
AccIII TCCGGA 1 cut(s) 497
AciI CCGC 6 cut(s) 39, 92, 325, 394, 538, 553
AclWI GGATC 1 cut(s) 379
AcoI YGGCCR 1 cut(s) 177
AcuI CTGAAG 1 cut(s) 710
AcyI GRCGYC 4 cut(s) 303, 423, 492, 617
AfaI GTAC 2 cut(s) 24, 632
AfiI CCNNNNNNNGG 3 cut(s) 359, 403, 681
AgsI TTSAA 4 cut(s) 250, 472, 550, 600
AjnI CCWGG 3 cut(s) 270, 282, 402
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
Alw21I GWGCWC 2 cut(s) 592, 717
Alw26I GTCTC 2 cut(s) 61, 499
AlwI GGATC 1 cut(s) 379
Ama87I CYCGRG 1 cut(s) 650
Aor13HI TCCGGA 1 cut(s) 497
AoxI GGCC 3 cut(s) 159, 177, 285
ApeKI GCWGC 1 cut(s) 415
AspS9I GGNCC 3 cut(s) 328, 573, 666
AsuC2I CCSGG 5 cut(s) 230, 621, 651, 652, 747
AsuHPI GGTGA 4 cut(s) 79, 136, 259, 356
AvaI CYCGRG 1 cut(s) 650
AvaII GGWCC 3 cut(s) 328, 573, 666
BaeGI GKGCMC 1 cut(s) 742
BalI TGGCCA 1 cut(s) 179
BanI GGYRCC 2 cut(s) 605, 737
BanII GRGCYC 3 cut(s) 213, 592, 717
Bbv12I GWGCWC 2 cut(s) 592, 717
BbvI GCAGC 1 cut(s) 402
BceAI ACGGC 3 cut(s) 92, 290, 310
BciT130I CCWGG 3 cut(s) 272, 284, 404
BclI TGATCA 1 cut(s) 729
BcnI CCSGG 5 cut(s) 230, 621, 651, 652, 747
BcoDI GTCTC 2 cut(s) 61, 499
BisI GCNGC 2 cut(s) 416, 539
BlsI GCNGC 2 cut(s) 417, 540
Bme1390I CCNGG 8 cut(s) 230, 272, 284, 404, 621, 651, 652, 747
Bme18I GGWCC 3 cut(s) 328, 573, 666
BmeT110I CYCGRG 1 cut(s) 650
BmgT120I GGNCC 3 cut(s) 328, 573, 666
BmiI GGNNCC 6 cut(s) 329, 502, 574, 607, 667, 739
BmrFI CCNGG 8 cut(s) 230, 272, 284, 404, 621, 651, 652, 747
BmsI GCATC 2 cut(s) 174, 636
BoxI GACNNNNGTC 1 cut(s) 140
BpmI CTGGAG 2 cut(s) 353, 386
BpuMI CCSGG 5 cut(s) 230, 621, 651, 652, 747
Bsa29I ATCGAT 1 cut(s) 705
BsaHI GRCGYC 4 cut(s) 303, 423, 492, 617
BsaJI CCNNGG 4 cut(s) 270, 282, 568, 650
BsaWI WCCGGW 3 cut(s) 215, 336, 497
Bsc4I CCNNNNNNNGG 3 cut(s) 359, 403, 681
Bse118I RCCGGY 3 cut(s) 161, 321, 377
BseAI TCCGGA 1 cut(s) 497
BseBI CCWGG 3 cut(s) 272, 284, 404
BseCI ATCGAT 1 cut(s) 705
BseDI CCNNGG 4 cut(s) 270, 282, 568, 650
BseLI CCNNNNNNNGG 3 cut(s) 359, 403, 681
BseRI GAGGAG 2 cut(s) 35, 55
BseSI GKGCMC 1 cut(s) 742
BseXI GCAGC 1 cut(s) 402
Bsh1285I CGRYCG 1 cut(s) 134
BshFI GGCC 3 cut(s) 161, 179, 287
BshNI GGYRCC 2 cut(s) 605, 737
BshVI ATCGAT 1 cut(s) 705
BsiEI CGRYCG 1 cut(s) 134
BsiHKAI GWGCWC 2 cut(s) 592, 717
BsiHKCI CYCGRG 1 cut(s) 650
BslFI GGGAC 2 cut(s) 212, 679
BslI CCNNNNNNNGG 3 cut(s) 359, 403, 681
BsmAI GTCTC 2 cut(s) 61, 499
BsmBI CGTCTC 1 cut(s) 499
BsmFI GGGAC 2 cut(s) 212, 679
BsnI GGCC 3 cut(s) 161, 179, 287
BsoBI CYCGRG 1 cut(s) 650
Bsp1286I GDGCHC 4 cut(s) 213, 592, 717, 742
Bsp13I TCCGGA 1 cut(s) 497
Bsp1407I TGTACA 1 cut(s) 630
Bsp143I GATC 4 cut(s) 384, 678, 702, 729
BspACI CCGC 6 cut(s) 39, 92, 325, 394, 538, 553
BspANI GGCC 3 cut(s) 161, 179, 287
BspDI ATCGAT 1 cut(s) 705
BspEI TCCGGA 1 cut(s) 497
BspLI GGNNCC 6 cut(s) 329, 502, 574, 607, 667, 739
BspPI GGATC 1 cut(s) 379
BspT107I GGYRCC 2 cut(s) 605, 737
BsrBI CCGCTC 1 cut(s) 41
BsrFI RCCGGY 3 cut(s) 161, 321, 377
BsrGI TGTACA 1 cut(s) 630
BssAI RCCGGY 3 cut(s) 161, 321, 377
BssECI CCNNGG 4 cut(s) 270, 282, 568, 650
BssMI GATC 4 cut(s) 384, 678, 702, 729
BssNI GRCGYC 4 cut(s) 303, 423, 492, 617
Bst2UI CCWGG 3 cut(s) 272, 284, 404
Bst4CI ACNGT 1 cut(s) 238
BstACI GRCGYC 4 cut(s) 303, 423, 492, 617
BstAUI TGTACA 1 cut(s) 630
BstC8I GCNNGC 3 cut(s) 163, 379, 757
BstDSI CCRYGG 1 cut(s) 568
BstEII GGTNACC 2 cut(s) 232, 362
BstKTI GATC 4 cut(s) 387, 681, 705, 732
BstMAI GTCTC 2 cut(s) 61, 499
BstMBI GATC 4 cut(s) 384, 678, 702, 729
BstMCI CGRYCG 1 cut(s) 134
BstMWI GCNNNNNNNGC 2 cut(s) 198, 349
BstNI CCWGG 3 cut(s) 272, 284, 404
BstPAI GACNNNNGTC 1 cut(s) 140
BstPI GGTNACC 2 cut(s) 232, 362
BstSCI CCNGG 8 cut(s) 228, 270, 282, 402, 619, 649, 650, 745
BstSLI GKGCMC 1 cut(s) 742
BstV1I GCAGC 1 cut(s) 402
BstXI CCANNNNNNTGG 1 cut(s) 154
Bsu15I ATCGAT 1 cut(s) 705
BsuRI GGCC 3 cut(s) 161, 179, 287
BsuTUI ATCGAT 1 cut(s) 705
BtgI CCRYGG 1 cut(s) 568
Cac8I GCNNGC 3 cut(s) 163, 379, 757
Cfr10I RCCGGY 3 cut(s) 161, 321, 377
Cfr13I GGNCC 3 cut(s) 328, 573, 666
Cfr9I CCCGGG 1 cut(s) 650
ClaI ATCGAT 1 cut(s) 705
CseI GACGC 2 cut(s) 311, 625
Csp6I GTAC 2 cut(s) 23, 631
CviAII CATG 4 cut(s) 148, 154, 175, 193
CviQI GTAC 2 cut(s) 23, 631
DpnI GATC 4 cut(s) 386, 680, 704, 731
DpnII GATC 4 cut(s) 384, 678, 702, 729
DrdI GACNNNNNNGTC 1 cut(s) 571
DseDI GACNNNNNNGTC 1 cut(s) 571
EaeI YGGCCR 1 cut(s) 177
EciI GGCGGA 1 cut(s) 383
Ecl136II GAGCTC 2 cut(s) 590, 715
Eco24I GRGCYC 3 cut(s) 213, 592, 717
Eco47I GGWCC 3 cut(s) 328, 573, 666
Eco53kI GAGCTC 2 cut(s) 590, 715
Eco57I CTGAAG 1 cut(s) 710
Eco88I CYCGRG 1 cut(s) 650
Eco91I GGTNACC 2 cut(s) 232, 362
EcoICRI GAGCTC 2 cut(s) 590, 715
EcoO65I GGTNACC 2 cut(s) 232, 362
EcoRII CCWGG 3 cut(s) 270, 282, 402
EcoT38I GRGCYC 3 cut(s) 213, 592, 717
Esp3I CGTCTC 1 cut(s) 499
FaeI CATG 4 cut(s) 151, 157, 178, 196
FaiI YATR 4 cut(s) 149, 155, 176, 194
FalI AAGNNNNNCTT 2 cut(s) 440, 472
FaqI GGGAC 2 cut(s) 212, 679
FatI CATG 4 cut(s) 147, 153, 174, 192
FauI CCCGC 3 cut(s) 99, 318, 546
FbaI TGATCA 1 cut(s) 729
FblI GTMKAC 1 cut(s) 489
Fnu4HI GCNGC 2 cut(s) 416, 539
FriOI GRGCYC 3 cut(s) 213, 592, 717
Fsp4HI GCNGC 2 cut(s) 416, 539
GluI GCNGC 2 cut(s) 416, 539
GsuI CTGGAG 2 cut(s) 353, 386
HaeIII GGCC 3 cut(s) 161, 179, 287
HgaI GACGC 2 cut(s) 311, 625
Hin1I GRCGYC 4 cut(s) 303, 423, 492, 617
Hin1II CATG 4 cut(s) 151, 157, 178, 196
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HinfI GANTC 4 cut(s) 141, 171, 432, 531
HphI GGTGA 4 cut(s) 79, 136, 259, 356
Hpy166II GTNNAC 2 cut(s) 490, 631
Hpy188I TCNGA 2 cut(s) 170, 512
Hpy188III TCNNGA 3 cut(s) 370, 498, 682
Hpy8I GTNNAC 2 cut(s) 490, 631
HpyAV CCTTC 6 cut(s) 36, 70, 422, 466, 618, 744
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4IV ACGT 2 cut(s) 423, 492
HpyCH4V TGCA 1 cut(s) 418
HpyF10VI GCNNNNNNNGC 2 cut(s) 198, 349
HpySE526I ACGT 2 cut(s) 423, 492
Hsp92I GRCGYC 4 cut(s) 303, 423, 492, 617
Hsp92II CATG 4 cut(s) 151, 157, 178, 196
Kpn2I TCCGGA 1 cut(s) 497
KroI GCCGGC 2 cut(s) 161, 377
KroNI GCCGGC 2 cut(s) 163, 379
Ksp22I TGATCA 1 cut(s) 729
Kzo9I GATC 4 cut(s) 384, 678, 702, 729
LmnI GCTCC 2 cut(s) 712, 771
Lsp1109I GCAGC 1 cut(s) 402
LweI GCATC 2 cut(s) 174, 636
MaeII ACGT 2 cut(s) 423, 492
MaeIII GTNAC 5 cut(s) 142, 232, 265, 332, 362
MalI GATC 4 cut(s) 386, 680, 704, 731
MbiI CCGCTC 1 cut(s) 41
MboI GATC 4 cut(s) 384, 678, 702, 729
MboII GAAGA 7 cut(s) 21, 24, 196, 652, 655, 703, 712
MhlI GDGCHC 4 cut(s) 213, 592, 717, 742
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 1 cut(s) 544
MluNI TGGCCA 1 cut(s) 179
MlyI GAGTC 2 cut(s) 150, 180
Mox20I TGGCCA 1 cut(s) 179
MroI TCCGGA 1 cut(s) 497
MroNI GCCGGC 2 cut(s) 161, 377
MscI TGGCCA 1 cut(s) 179
MslI CAYNNNNRTG 1 cut(s) 152
Msp20I TGGCCA 1 cut(s) 179
MspR9I CCNGG 8 cut(s) 230, 272, 284, 404, 621, 651, 652, 747
MvaI CCWGG 3 cut(s) 272, 284, 404
MwoI GCNNNNNNNGC 2 cut(s) 198, 349
NaeI GCCGGC 2 cut(s) 163, 379
NciI CCSGG 5 cut(s) 230, 621, 651, 652, 747
NdeII GATC 4 cut(s) 384, 678, 702, 729
NgoMIV GCCGGC 2 cut(s) 161, 377
NlaIII CATG 4 cut(s) 151, 157, 178, 196
NlaIV GGNNCC 6 cut(s) 329, 502, 574, 607, 667, 739
NmeAIII GCCGAG 1 cut(s) 168
NmuCI GTSAC 4 cut(s) 142, 265, 332, 362
PcsI WCGNNNNNNNCGW 1 cut(s) 432
PdiI GCCGGC 2 cut(s) 163, 379
PfeI GAWTC 2 cut(s) 432, 531
PflFI GACNNNGTC 2 cut(s) 305, 485
PfoI TCCNGGA 1 cut(s) 402
PkrI GCNGC 2 cut(s) 417, 540
PleI GAGTC 2 cut(s) 149, 179
PpsI GAGTC 2 cut(s) 149, 179
PshAI GACNNNNGTC 1 cut(s) 140
Psp124BI GAGCTC 2 cut(s) 592, 717
Psp6I CCWGG 3 cut(s) 270, 282, 402
PspEI GGTNACC 2 cut(s) 232, 362
PspGI CCWGG 3 cut(s) 270, 282, 402
PspN4I GGNNCC 6 cut(s) 329, 502, 574, 607, 667, 739
PspPI GGNCC 3 cut(s) 328, 573, 666
PsyI GACNNNGTC 2 cut(s) 305, 485
RsaI GTAC 2 cut(s) 24, 632
RsaNI GTAC 2 cut(s) 23, 631
RseI CAYNNNNRTG 1 cut(s) 152
SacI GAGCTC 2 cut(s) 592, 717
SalI GTCGAC 1 cut(s) 488
SatI GCNGC 2 cut(s) 416, 539
Sau3AI GATC 4 cut(s) 384, 678, 702, 729
Sau96I GGNCC 3 cut(s) 328, 573, 666
SchI GAGTC 2 cut(s) 150, 180
ScrFI CCNGG 8 cut(s) 230, 272, 284, 404, 621, 651, 652, 747
SduI GDGCHC 4 cut(s) 213, 592, 717, 742
SfaNI GCATC 2 cut(s) 174, 636
SgrDI CGTCGACG 1 cut(s) 488
SinI GGWCC 3 cut(s) 328, 573, 666
SmaI CCCGGG 1 cut(s) 652
SmiMI CAYNNNNRTG 1 cut(s) 152
Sse9I AATT 1 cut(s) 544
SsiI CCGC 6 cut(s) 39, 92, 325, 394, 538, 553
SstI GAGCTC 2 cut(s) 592, 717
StyD4I CCNGG 8 cut(s) 228, 270, 282, 402, 619, 649, 650, 745
TaaI ACNGT 1 cut(s) 238
TaiI ACGT 2 cut(s) 426, 495
TaqI TCGA 7 cut(s) 207, 383, 426, 435, 459, 489, 705
TaqII GACCGA 1 cut(s) 148
TasI AATT 1 cut(s) 544
TatI WGTACW 1 cut(s) 630
TauI GCSGC 1 cut(s) 541
TfiI GAWTC 2 cut(s) 432, 531
TseFI GTSAC 4 cut(s) 142, 265, 332, 362
TseI GCWGC 1 cut(s) 415
Tsp45I GTSAC 4 cut(s) 142, 265, 332, 362
TspGWI ACGGA 1 cut(s) 320
TspMI CCCGGG 1 cut(s) 650
Tth111I GACNNNGTC 2 cut(s) 305, 485
VpaK11BI GGWCC 3 cut(s) 328, 573, 666
XmaI CCCGGG 1 cut(s) 650
XmiI GTMKAC 1 cut(s) 489
ZraI GACGTC 2 cut(s) 424, 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.