AT2G44530

Ribose-phosphate pyrophosphokinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008289
Physical Location & Seq
Forward (+)
18383515 .. 18386208
2694 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44530.2

Sequence Viewer

Length: 1185 bp
ATGGCGTCTATTGTTCAACCATCGCCTACTTTCCCGGCGCTAAATCTCCGGCGTTCTTCTCTGATTCGTCCGCCTTCTTCCGTTCGATTTCCTCTTAAGTGTAACGCGGCGGATCCGTACAAGTTCGACGGCGGAAACTCTGCCGGTTTCCATCTGCTTACTGGCGACACCGTTCCGGCTAGCTTTTCGAGGACACGTTTGGAAGATTCGATTTATCAGAACACCACACGACTTCGTATCTTTTCCGGCACTGCTAATCCTATTTTGGCTCAGGAGATTTCTTGCTATTTGGGTCTGGACCTTGGGAAAATCAAGATTAAACGCTTTGCTGATGGTGAGATCTATGTTCAGCTACAAGAGAGTGTAAGGGGATGTGATGTGTTCCTTGTACAGCCTACATGCCCACCTGCAAATGAAAACCTTATGGAATTGCTCGTTATGATTGATGCTTGTCGGAGAGCATCAGCCAAAACTATCACGGCTGTGATTCCTTACTTTGGTTATGCACGAGCTGATAGAAAGACTCAAGGACGTGAATCTATTGCAGCCAAGCTTGTGGCCAATTTGATTACACAGTCTGGTGCAGACCGTGTCCTTGCTTGTGATCTTCACTCCGGACAGTCTATGGGCTACTTTGATATTCCAGTCGATCATGTTTATGGCCAGCCTGTCATACTTGATTACCTAGCAAGTAAGGCCATATCCTCTGAAGATTTGGTGGTAGTTTCACCTGATGTTGGTGGCGTCGCAAGAGCTCGCGCTTTTGCGAAGAAGTTATCTGATGCACCTTTAGCAATAGTTGATAAAAGACGTCATGGGCACAATGTTGCAGAGGTGATGAACTTAATTGGGGATGTTAAAGGGAAAGTAGCCATAATGGTGGATGACATGATTGACACAGCAGGAACCATAAGCAAAGGTGCGGCTCTGTTACACCAAGAAGGAGCAAGAGAAGTATACGCTTGTACCACTCATGCCGTTTTTAGCCCTCCTGCAATCAGTCGACTATCGAGCGGACTGTTTCAAGAGGTGATCATAACCAACACGATTCCATTGTCAGAGAAGAACTATTTTCCTCAGCTTACAGTTCTCTCAGTAGCAAACCTTCTTGGGGAGACCATATGGCGTGTTCATGATGATTGCTCGGGAGCCATTGAGCCATTTTCGACGTTGGGGATTGATTGA

Protein Analysis

394

Amino Acids

42.62

Weight (kDa)

6.71

Isoelectric Point (pI)

45.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pribosyltran_N PF13793 78 - 193 8.4e-52 N-terminal domain of ribose phosphate pyrophosphokinase
Pribosyltran PF00156 229 - 325 2.3e-17 Phosphoribosyl transferase domain
Pribosyl_synth PF14572 278 - 381 3.5e-22 Phosphoribosyl synthetase-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 415
AatII GACGTC 1 cut(s) 814
Acc36I ACCTGC 1 cut(s) 415
AccBSI CCGCTC 1 cut(s) 1014
AccI GTMKAC 2 cut(s) 957, 1003
AccII CGCG 2 cut(s) 107, 759
AccIII TCCGGA 1 cut(s) 614
AciI CCGC 6 cut(s) 71, 107, 110, 132, 923, 1014
AclWI GGATC 2 cut(s) 107, 120
AcoI YGGCCR 2 cut(s) 558, 661
AcuI CTGAAG 1 cut(s) 729
AcyI GRCGYC 3 cut(s) 5, 744, 811
AfaI GTAC 3 cut(s) 119, 390, 967
AfiI CCNNNNNNNGG 3 cut(s) 497, 737, 1111
AflII CTTAAG 1 cut(s) 95
AflIII ACRYGT 1 cut(s) 194
AgsI TTSAA 2 cut(s) 17, 1025
AjiI CACGTC 1 cut(s) 533
AleI CACNNNNGTG 1 cut(s) 482
AluBI AGCT 6 cut(s) 183, 352, 512, 553, 755, 1081
AluI AGCT 6 cut(s) 183, 352, 512, 553, 755, 1081
Alw21I GWGCWC 1 cut(s) 757
Alw26I GTCTC 1 cut(s) 1109
AlwI GGATC 2 cut(s) 107, 120
Ama87I CYCGRG 1 cut(s) 1144
Aor13HI TCCGGA 1 cut(s) 614
AoxI GGCC 3 cut(s) 558, 661, 696
ApeKI GCWGC 1 cut(s) 545
AspLEI GCGC 2 cut(s) 40, 761
AspS9I GGNCC 1 cut(s) 298
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 4 cut(s) 347, 720, 847, 1042
AsuNHI GCTAGC 1 cut(s) 179
AvaI CYCGRG 1 cut(s) 1144
AvaII GGWCC 1 cut(s) 298
BaeGI GKGCMC 1 cut(s) 822
BaeI ACNNNNGTAYC 2 cut(s) 949, 982
BalI TGGCCA 2 cut(s) 560, 663
BamHI GGATCC 1 cut(s) 112
BanII GRGCYC 1 cut(s) 757
BarI GAAGNNNNNNTAC 2 cut(s) 1089, 1121
BauI CACGAG 1 cut(s) 507
Bbv12I GWGCWC 1 cut(s) 757
BbvCI CCTCAGC 1 cut(s) 1077
BbvI GCAGC 1 cut(s) 557
BccI CCATC 3 cut(s) 28, 159, 326
BceAI ACGGC 3 cut(s) 145, 495, 962
BclI TGATCA 1 cut(s) 1032
BcnI CCSGG 1 cut(s) 35
BcoDI GTCTC 1 cut(s) 1109
BfaI CTAG 2 cut(s) 180, 686
BfoI RGCGCY 1 cut(s) 41
BfrI CTTAAG 1 cut(s) 95
BfuAI ACCTGC 1 cut(s) 415
BglII AGATCT 1 cut(s) 339
BisI GCNGC 3 cut(s) 108, 546, 924
BlsI GCNGC 3 cut(s) 109, 547, 925
Bme1390I CCNGG 1 cut(s) 35
Bme18I GGWCC 1 cut(s) 298
BmeT110I CYCGRG 1 cut(s) 1144
BmgBI CACGTC 1 cut(s) 533
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 3 cut(s) 114, 907, 1150
BmrFI CCNGG 1 cut(s) 35
BmsI GCATC 3 cut(s) 436, 470, 772
BmtI GCTAGC 1 cut(s) 183
Bpu10I CCTNAGC 2 cut(s) 270, 1077
BpuEI CTTGAG 1 cut(s) 510
BpuMI CCSGG 1 cut(s) 35
BsaHI GRCGYC 3 cut(s) 5, 744, 811
BsaI GGTCTC 1 cut(s) 1109
BsaJI CCNNGG 1 cut(s) 301
BsaWI WCCGGW 1 cut(s) 614
Bsc4I CCNNNNNNNGG 3 cut(s) 497, 737, 1111
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 2 cut(s) 166, 644
BseAI TCCGGA 1 cut(s) 614
BseDI CCNNGG 1 cut(s) 301
BseGI GGATG 3 cut(s) 377, 859, 889
BseLI CCNNNNNNNGG 3 cut(s) 497, 737, 1111
BseMII CTCAG 3 cut(s) 284, 1091, 1107
BseNI ACTGG 2 cut(s) 166, 644
BseSI GKGCMC 1 cut(s) 822
BseXI GCAGC 1 cut(s) 557
BsgI GTGCAG 1 cut(s) 603
Bsh1236I CGCG 2 cut(s) 107, 759
BshFI GGCC 3 cut(s) 560, 663, 698
BsiHKAI GWGCWC 1 cut(s) 757
BsiHKCI CYCGRG 1 cut(s) 1144
BsiSI CCGG 6 cut(s) 35, 49, 144, 176, 246, 615
BslI CCNNNNNNNGG 3 cut(s) 497, 737, 1111
BsmAI GTCTC 1 cut(s) 1109
BsnI GGCC 3 cut(s) 560, 663, 698
Bso31I GGTCTC 1 cut(s) 1109
BsoBI CYCGRG 1 cut(s) 1144
Bsp1286I GDGCHC 2 cut(s) 757, 822
Bsp13I TCCGGA 1 cut(s) 614
Bsp1407I TGTACA 1 cut(s) 388
Bsp143I GATC 5 cut(s) 112, 339, 604, 649, 1032
BspACI CCGC 6 cut(s) 71, 107, 110, 132, 923, 1014
BspANI GGCC 3 cut(s) 560, 663, 698
BspCNI CTCAG 3 cut(s) 283, 1090, 1106
BspEI TCCGGA 1 cut(s) 614
BspFNI CGCG 2 cut(s) 107, 759
BspHI TCATGA 1 cut(s) 1132
BspLI GGNNCC 3 cut(s) 114, 907, 1150
BspMI ACCTGC 1 cut(s) 415
BspOI GCTAGC 1 cut(s) 183
BspPI GGATC 2 cut(s) 107, 120
BspTI CTTAAG 1 cut(s) 95
BspTNI GGTCTC 1 cut(s) 1109
BsrBI CCGCTC 1 cut(s) 1014
BsrFI RCCGGY 1 cut(s) 143
BsrGI TGTACA 1 cut(s) 388
BsrI ACTGG 2 cut(s) 166, 644
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 5 cut(s) 112, 339, 604, 649, 1032
BssNAI GTATAC 1 cut(s) 958
BssNI GRCGYC 3 cut(s) 5, 744, 811
BssSI CACGAG 1 cut(s) 507
BssT1I CCWWGG 1 cut(s) 301
Bst1107I GTATAC 1 cut(s) 958
Bst2BI CACGAG 1 cut(s) 507
Bst4CI ACNGT 6 cut(s) 172, 576, 590, 621, 1020, 1087
BstACI GRCGYC 3 cut(s) 5, 744, 811
BstAFI CTTAAG 1 cut(s) 95
BstAUI TGTACA 1 cut(s) 388
BstC8I GCNNGC 3 cut(s) 181, 665, 757
BstDEI CTNAG 3 cut(s) 270, 1077, 1093
BstF5I GGATG 3 cut(s) 377, 859, 889
BstFNI CGCG 2 cut(s) 107, 759
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 2 cut(s) 40, 761
BstKTI GATC 5 cut(s) 115, 342, 607, 652, 1035
BstMAI GTCTC 1 cut(s) 1109
BstMBI GATC 5 cut(s) 112, 339, 604, 649, 1032
BstMWI GCNNNNNNNGC 2 cut(s) 695, 791
BstNSI RCATGY 1 cut(s) 402
BstSCI CCNGG 1 cut(s) 33
BstSLI GKGCMC 1 cut(s) 822
BstUI CGCG 2 cut(s) 107, 759
BstV1I GCAGC 1 cut(s) 557
BstX2I RGATCY 2 cut(s) 112, 339
BstXI CCANNNNNNTGG 2 cut(s) 556, 880
BstYI RGATCY 2 cut(s) 112, 339
BstZ17I GTATAC 1 cut(s) 958
BsuRI GGCC 3 cut(s) 560, 663, 698
BtgZI GCGATG 1 cut(s) 6
BtrI CACGTC 1 cut(s) 533
BtsCI GGATG 3 cut(s) 377, 859, 889
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 1 cut(s) 249
BveI ACCTGC 1 cut(s) 415
Cac8I GCNNGC 3 cut(s) 181, 665, 757
CciI TCATGA 1 cut(s) 1132
CfoI GCGC 2 cut(s) 40, 761
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 1 cut(s) 298
CseI GACGC 1 cut(s) 733
Csp6I GTAC 3 cut(s) 118, 389, 966
CviAII CATG 6 cut(s) 399, 653, 815, 889, 974, 1133
CviQI GTAC 3 cut(s) 118, 389, 966
DdeI CTNAG 3 cut(s) 270, 1077, 1093
DpnI GATC 5 cut(s) 114, 341, 606, 651, 1034
DpnII GATC 5 cut(s) 112, 339, 604, 649, 1032
EaeI YGGCCR 2 cut(s) 558, 661
EciI GGCGGA 3 cut(s) 60, 125, 147
Ecl136II GAGCTC 1 cut(s) 755
Eco130I CCWWGG 1 cut(s) 301
Eco24I GRGCYC 1 cut(s) 757
Eco31I GGTCTC 1 cut(s) 1109
Eco47I GGWCC 1 cut(s) 298
Eco53kI GAGCTC 1 cut(s) 755
Eco57I CTGAAG 1 cut(s) 729
Eco88I CYCGRG 1 cut(s) 1144
EcoICRI GAGCTC 1 cut(s) 755
EcoT14I CCWWGG 1 cut(s) 301
EcoT38I GRGCYC 1 cut(s) 757
ErhI CCWWGG 1 cut(s) 301
FaeI CATG 6 cut(s) 402, 656, 818, 892, 977, 1136
FatI CATG 6 cut(s) 398, 652, 814, 888, 973, 1132
FauNDI CATATG 1 cut(s) 1121
FbaI TGATCA 1 cut(s) 1032
FblI GTMKAC 2 cut(s) 957, 1003
Fnu4HI GCNGC 3 cut(s) 108, 546, 924
FokI GGATG 3 cut(s) 384, 866, 896
FriOI GRGCYC 1 cut(s) 757
Fsp4HI GCNGC 3 cut(s) 108, 546, 924
FspBI CTAG 2 cut(s) 180, 686
GlaI GCGC 2 cut(s) 39, 760
GluI GCNGC 3 cut(s) 108, 546, 924
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 3 cut(s) 560, 663, 698
HapII CCGG 6 cut(s) 35, 49, 144, 176, 246, 615
HgaI GACGC 1 cut(s) 733
HhaI GCGC 2 cut(s) 40, 761
Hin1I GRCGYC 3 cut(s) 5, 744, 811
Hin1II CATG 6 cut(s) 402, 656, 818, 892, 977, 1136
Hin6I GCGC 2 cut(s) 38, 759
HinP1I GCGC 2 cut(s) 38, 759
HincII GTYRAC 1 cut(s) 1004
HindII GTYRAC 1 cut(s) 1004
HindIII AAGCTT 1 cut(s) 551
HinfI GANTC 6 cut(s) 64, 206, 487, 523, 536, 1048
HpaII CCGG 6 cut(s) 35, 49, 144, 176, 246, 615
HphI GGTGA 4 cut(s) 347, 720, 847, 1042
Hpy166II GTNNAC 2 cut(s) 958, 1004
Hpy188I TCNGA 6 cut(s) 63, 219, 456, 709, 781, 1060
Hpy188III TCNNGA 7 cut(s) 272, 296, 313, 615, 1025, 1133, 1146
Hpy8I GTNNAC 2 cut(s) 958, 1004
Hpy99I CGWCG 3 cut(s) 131, 749, 1171
HpyAV CCTTC 3 cut(s) 84, 935, 1115
HpyCH4III ACNGT 6 cut(s) 172, 576, 590, 621, 1020, 1087
HpyCH4IV ACGT 4 cut(s) 196, 532, 811, 1169
HpyCH4V TGCA 7 cut(s) 410, 506, 545, 584, 785, 830, 995
HpyF10VI GCNNNNNNNGC 2 cut(s) 695, 791
HpyF3I CTNAG 3 cut(s) 270, 1077, 1093
HpySE526I ACGT 4 cut(s) 196, 532, 811, 1169
Hsp92I GRCGYC 3 cut(s) 5, 744, 811
Hsp92II CATG 6 cut(s) 402, 656, 818, 892, 977, 1136
HspAI GCGC 2 cut(s) 38, 759
Kpn2I TCCGGA 1 cut(s) 614
Ksp22I TGATCA 1 cut(s) 1032
Kzo9I GATC 5 cut(s) 112, 339, 604, 649, 1032
LmnI GCTCC 2 cut(s) 944, 1148
Lsp1109I GCAGC 1 cut(s) 557
LweI GCATC 3 cut(s) 436, 470, 772
MaeI CTAG 2 cut(s) 180, 686
MaeII ACGT 4 cut(s) 196, 532, 811, 1169
MaeIII GTNAC 2 cut(s) 101, 930
MalI GATC 5 cut(s) 114, 341, 606, 651, 1034
MbiI CCGCTC 1 cut(s) 1014
MboI GATC 5 cut(s) 112, 339, 604, 649, 1032
MboII GAAGA 7 cut(s) 48, 69, 215, 599, 722, 781, 1075
MflI RGATCY 2 cut(s) 112, 339
MhlI GDGCHC 2 cut(s) 757, 822
MlsI TGGCCA 2 cut(s) 560, 663
MluCI AATT 3 cut(s) 428, 562, 846
MluNI TGGCCA 2 cut(s) 560, 663
MlyI GAGTC 1 cut(s) 517
MmeI TCCRAC 1 cut(s) 434
MnlI CCTC 7 cut(s) 102, 183, 715, 826, 999, 1021, 1086
Mox20I TGGCCA 2 cut(s) 560, 663
MroI TCCGGA 1 cut(s) 614
MscI TGGCCA 2 cut(s) 560, 663
MseI TTAA 4 cut(s) 96, 318, 845, 858
MslI CAYNNNNRTG 3 cut(s) 482, 657, 878
Msp20I TGGCCA 2 cut(s) 560, 663
MspCI CTTAAG 1 cut(s) 95
MspI CCGG 6 cut(s) 35, 49, 144, 176, 246, 615
MspR9I CCNGG 1 cut(s) 35
MvnI CGCG 2 cut(s) 107, 759
MwoI GCNNNNNNNGC 2 cut(s) 695, 791
NciI CCSGG 1 cut(s) 35
NdeI CATATG 1 cut(s) 1121
NdeII GATC 5 cut(s) 112, 339, 604, 649, 1032
NheI GCTAGC 1 cut(s) 179
NlaIII CATG 6 cut(s) 402, 656, 818, 892, 977, 1136
NlaIV GGNNCC 3 cut(s) 114, 907, 1150
NspI RCATGY 1 cut(s) 402
OliI CACNNNNGTG 1 cut(s) 482
PagI TCATGA 1 cut(s) 1132
PaqCI CACCTGC 1 cut(s) 415
PfeI GAWTC 5 cut(s) 64, 206, 487, 536, 1048
PflFI GACNNNGTC 1 cut(s) 590
PkrI GCNGC 3 cut(s) 109, 547, 925
PleI GAGTC 1 cut(s) 517
PpsI GAGTC 1 cut(s) 517
Psp124BI GAGCTC 1 cut(s) 757
PspN4I GGNNCC 3 cut(s) 114, 907, 1150
PspPI GGNCC 1 cut(s) 298
PsuI RGATCY 2 cut(s) 112, 339
PsyI GACNNNGTC 1 cut(s) 590
RsaI GTAC 3 cut(s) 119, 390, 967
RsaNI GTAC 3 cut(s) 118, 389, 966
RseI CAYNNNNRTG 3 cut(s) 482, 657, 878
SacI GAGCTC 1 cut(s) 757
SalI GTCGAC 1 cut(s) 1002
SaqAI TTAA 4 cut(s) 96, 318, 845, 858
SatI GCNGC 3 cut(s) 108, 546, 924
Sau3AI GATC 5 cut(s) 112, 339, 604, 649, 1032
Sau96I GGNCC 1 cut(s) 298
SchI GAGTC 1 cut(s) 517
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 2 cut(s) 757, 822
SfaNI GCATC 3 cut(s) 436, 470, 772
SinI GGWCC 1 cut(s) 298
SmiMI CAYNNNNRTG 3 cut(s) 482, 657, 878
SmlI CTYRAG 2 cut(s) 95, 525
SmoI CTYRAG 2 cut(s) 95, 525
Sse9I AATT 3 cut(s) 428, 562, 846
SsiI CCGC 6 cut(s) 71, 107, 110, 132, 923, 1014
SspMI CTAG 2 cut(s) 180, 686
SstI GAGCTC 1 cut(s) 757
StyD4I CCNGG 1 cut(s) 33
StyI CCWWGG 1 cut(s) 301
TaaI ACNGT 6 cut(s) 172, 576, 590, 621, 1020, 1087
TaiI ACGT 4 cut(s) 199, 535, 814, 1172
TaqI TCGA 8 cut(s) 85, 126, 188, 209, 648, 1003, 1010, 1166
TasI AATT 3 cut(s) 428, 562, 846
TatI WGTACW 1 cut(s) 388
TauI GCSGC 2 cut(s) 110, 926
TfiI GAWTC 5 cut(s) 64, 206, 487, 536, 1048
Tru1I TTAA 4 cut(s) 96, 318, 845, 858
Tru9I TTAA 4 cut(s) 96, 318, 845, 858
TscAI CASTG 1 cut(s) 256
TseI GCWGC 1 cut(s) 545
TspDTI ATGAA 3 cut(s) 429, 854, 1121
TspGWI ACGGA 2 cut(s) 70, 105
TspRI CASTG 1 cut(s) 256
Tth111I GACNNNGTC 1 cut(s) 590
Vha464I CTTAAG 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 298
XceI RCATGY 1 cut(s) 402
XcmI CCANNNNNNNNNTGG 1 cut(s) 158
XmiI GTMKAC 2 cut(s) 957, 1003
XspI CTAG 2 cut(s) 180, 686
ZraI GACGTC 1 cut(s) 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.