MD07G1084000.v1.1

Ribose-phosphate pyrophosphokinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008289
Physical Location & Seq
Reverse (-)
8636966 .. 8643905
6940 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1084000.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGGCGTCGCTGCTCCACACAGCGTCCCGGTCCTCTCTCGCTTCTCGCGACTCTCTACGCCATCGCACTTACCTCCCCAACTACTCTCGCTCTCAAATCCCCATCCTGAATTCCGTGAGGTGTGGTGTGGGAGAGCCTCTCAAGTATGCAAATGGAAAGCCGTGCGTACCACTCCTCAGGAGTGAAGATTTGGTGCCGACTTTTTCGGACACGAAAACACATTTGGTGAATGAAATTAGCAAGAATGATACCAGGCTCAGGATCTTTTCCGGCACTGCGAACCCTGCGCTGTCTCAGGAAATTGCTTGCTGCATGGGCCTGGAACTTGGAAGGATTAAGATCAAACGCTTTGCTGATGGAGAGATATATGTTCAGTTGCAAGAGAGTGTAAGGGGGTGTGATGTATATCTTGTGCAACCCACATGTCCTCCTGCAAATGAAAATCTTATGGAGCTTCTTGTCATGATTGATGCTTGTCGGAGGGCTTCAGCCAAGAATATCACTGCTGTGATTCCATATTTTGGCTATGCCAGGGCTGATAGAAAGACTCAAGGGCGTGAATCTATTGCAGCCAAACTTGTTGCAAATTTGATTACAGAAGCAGGTGCAAATCGTGTTCTTGCATGTGATCTTCATTCTGGGCAGTCTATGGGATATTTTGATATTCCTGTGGATCATGTATATGGTCAGCCTGTGATACTTGATTATCTCGCCAGCAAGACTATATACTCTGATGATTTGGTAGTGGTCTCACCCGATGTTGGGGGTGTTGCTCGAGCTCGTGCCTTCGCGAAAAAATTATCTGATGCTCCTTTAGCCATTGTTGATAAAAGACGTCACGGGCACAATGTGGCAGAGGTGATGAATTTGATAGGTGATGTCAAGGGAAAAGTAGCAGTTATGGTGGATGACATGATTGACACAGCTGGGACCATTACAAAAGGTGCTGCTTTACTACACCAAGAGGGTGCCAGGGAAGTCTATGCATGTTGTACGCATGCGGTTTTTAGCCCTCCTGCTATAGAGAGGCTGTCGAGCGGTTTGTTTCAAGAGGTGATCATAACAAACACCATTCCAGTGTCGGAGCAGAATTATTTTCCCCAGCTAACTGTCTTGTCAGTAGCGAATCTTCTGGGCGAGACCATATGGCGTGTTCATGATGACTGCTCTGGTGGCTATGAACCCTATTCCACCTTGGGCATTGATTGA

Protein Analysis

403

Amino Acids

43.81

Weight (kDa)

6.56

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pribosyltran_N PF13793 87 - 202 8.1e-52 N-terminal domain of ribose phosphate pyrophosphokinase
Pribosyltran PF00156 245 - 334 1.5e-17 Phosphoribosyl transferase domain
Pribosyl_synth PF14572 287 - 393 1.7e-22 Phosphoribosyl synthetase-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 593
AatII GACGTC 1 cut(s) 838
Acc36I ACCTGC 1 cut(s) 593
AccB1I GGYRCC 2 cut(s) 193, 968
AccB7I CCANNNNNTGG 1 cut(s) 521
AccBSI CCGCTC 1 cut(s) 1038
AccII CGCG 2 cut(s) 48, 791
AciI CCGC 2 cut(s) 1001, 1038
AclWI GGATC 2 cut(s) 269, 681
AcsI RAATTY 3 cut(s) 109, 586, 865
AcuI CTGAAG 1 cut(s) 471
AcyI GRCGYC 2 cut(s) 5, 835
AdeI CACNNNGTG 1 cut(s) 850
AfaI GTAC 2 cut(s) 168, 994
AfiI CCNNNNNNNGG 4 cut(s) 258, 521, 761, 762
AflIII ACRYGT 1 cut(s) 422
AgsI TTSAA 1 cut(s) 1049
AjnI CCWGG 4 cut(s) 251, 318, 530, 971
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
AleI CACNNNNGTG 1 cut(s) 506
AluBI AGCT 4 cut(s) 454, 779, 926, 1105
AluI AGCT 4 cut(s) 454, 779, 926, 1105
Alw21I GWGCWC 1 cut(s) 781
Alw26I GTCTC 3 cut(s) 297, 754, 1133
AlwI GGATC 2 cut(s) 269, 681
Ama87I CYCGRG 1 cut(s) 774
AoxI GGCC 1 cut(s) 316
ApeKI GCWGC 4 cut(s) 10, 309, 569, 947
ApoI RAATTY 3 cut(s) 109, 586, 865
AspLEI GCGC 1 cut(s) 289
AspS9I GGNCC 3 cut(s) 30, 316, 930
AsuC2I CCSGG 1 cut(s) 28
AsuHPI GGTGA 5 cut(s) 238, 744, 871, 887, 1066
AvaI CYCGRG 1 cut(s) 774
AvaII GGWCC 2 cut(s) 30, 930
AxyI CCTNAGG 1 cut(s) 176
BaeGI GKGCMC 1 cut(s) 846
BanI GGYRCC 2 cut(s) 193, 968
BanII GRGCYC 1 cut(s) 781
BarI GAAGNNNNNNTAC 2 cut(s) 1113, 1145
BauI CACGAG 1 cut(s) 780
Bbv12I GWGCWC 1 cut(s) 781
BbvI GCAGC 3 cut(s) 296, 581, 934
BccI CCATC 3 cut(s) 69, 110, 350
BceAI ACGGC 1 cut(s) 145
BciT130I CCWGG 4 cut(s) 253, 320, 532, 973
BclI TGATCA 1 cut(s) 1056
BcnI CCSGG 1 cut(s) 28
BcoDI GTCTC 3 cut(s) 297, 754, 1133
BfmI CTRYAG 1 cut(s) 1020
BfuAI ACCTGC 1 cut(s) 593
BisI GCNGC 4 cut(s) 11, 310, 570, 948
BlsI GCNGC 4 cut(s) 12, 311, 571, 949
Bme1390I CCNGG 5 cut(s) 28, 253, 320, 532, 973
Bme18I GGWCC 2 cut(s) 30, 930
BmeT110I CYCGRG 1 cut(s) 774
BmgT120I GGNCC 3 cut(s) 30, 316, 930
BmiI GGNNCC 3 cut(s) 195, 931, 970
BmrFI CCNGG 5 cut(s) 28, 253, 320, 532, 973
BmsI GCATC 2 cut(s) 460, 796
BplI GAGNNNNNCTC 2 cut(s) 123, 155
Bpu10I CCTNAGC 1 cut(s) 257
BpuEI CTTGAG 2 cut(s) 125, 534
BpuMI CCSGG 1 cut(s) 28
BsaBI GATNNNNATC 2 cut(s) 338, 405
BsaHI GRCGYC 2 cut(s) 5, 835
BsaI GGTCTC 2 cut(s) 754, 1133
BsaJI CCNNGG 3 cut(s) 531, 972, 1194
BsaXI ACNNNNNCTCC 2 cut(s) 412, 442
Bsc4I CCNNNNNNNGG 4 cut(s) 258, 521, 761, 762
Bse1I ACTGG 1 cut(s) 1076
Bse21I CCTNAGG 1 cut(s) 176
Bse8I GATNNNNATC 2 cut(s) 338, 405
BseBI CCWGG 4 cut(s) 253, 320, 532, 973
BseDI CCNNGG 3 cut(s) 531, 972, 1194
BseGI GGATG 2 cut(s) 102, 913
BseJI GATNNNNATC 2 cut(s) 338, 405
BseLI CCNNNNNNNGG 4 cut(s) 258, 521, 761, 762
BseMII CTCAG 3 cut(s) 190, 271, 308
BseNI ACTGG 1 cut(s) 1076
BseRI GAGGAG 1 cut(s) 164
BseSI GKGCMC 1 cut(s) 846
BseXI GCAGC 3 cut(s) 296, 581, 934
BseYI CCCAGC 2 cut(s) 926, 1101
Bsh1236I CGCG 2 cut(s) 48, 791
BshFI GGCC 1 cut(s) 318
BshNI GGYRCC 2 cut(s) 193, 968
BsiHKAI GWGCWC 1 cut(s) 781
BsiHKCI CYCGRG 1 cut(s) 774
BsiSI CCGG 2 cut(s) 28, 270
BslFI GGGAC 2 cut(s) 10, 943
BslI CCNNNNNNNGG 4 cut(s) 258, 521, 761, 762
BsmAI GTCTC 3 cut(s) 297, 754, 1133
BsmFI GGGAC 2 cut(s) 10, 943
BsnI GGCC 1 cut(s) 318
Bso31I GGTCTC 2 cut(s) 754, 1133
BsoBI CYCGRG 1 cut(s) 774
Bsp1286I GDGCHC 2 cut(s) 781, 846
Bsp143I GATC 5 cut(s) 261, 339, 628, 673, 1056
Bsp68I TCGCGA 2 cut(s) 48, 791
BspACI CCGC 2 cut(s) 1001, 1038
BspANI GGCC 1 cut(s) 318
BspCNI CTCAG 3 cut(s) 189, 270, 307
BspFNI CGCG 2 cut(s) 48, 791
BspHI TCATGA 2 cut(s) 462, 1156
BspLI GGNNCC 3 cut(s) 195, 931, 970
BspMI ACCTGC 1 cut(s) 593
BspPI GGATC 2 cut(s) 269, 681
BspT107I GGYRCC 2 cut(s) 193, 968
BspTNI GGTCTC 2 cut(s) 754, 1133
BsrBI CCGCTC 1 cut(s) 1038
BsrI ACTGG 1 cut(s) 1076
BssECI CCNNGG 3 cut(s) 531, 972, 1194
BssMI GATC 5 cut(s) 261, 339, 628, 673, 1056
BssNI GRCGYC 2 cut(s) 5, 835
BssSI CACGAG 1 cut(s) 780
BssT1I CCWWGG 1 cut(s) 1194
Bst2BI CACGAG 1 cut(s) 780
Bst2UI CCWGG 4 cut(s) 253, 320, 532, 973
Bst4CI ACNGT 1 cut(s) 1111
BstACI GRCGYC 2 cut(s) 5, 835
BstC8I GCNNGC 3 cut(s) 307, 715, 999
BstDEI CTNAG 3 cut(s) 176, 257, 294
BstF5I GGATG 2 cut(s) 102, 913
BstFNI CGCG 2 cut(s) 48, 791
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 5 cut(s) 264, 342, 631, 676, 1059
BstMAI GTCTC 3 cut(s) 297, 754, 1133
BstMBI GATC 5 cut(s) 261, 339, 628, 673, 1056
BstMWI GCNNNNNNNGC 4 cut(s) 284, 315, 815, 1173
BstNI CCWGG 4 cut(s) 253, 320, 532, 973
BstNSI RCATGY 4 cut(s) 426, 627, 990, 1001
BstSCI CCNGG 5 cut(s) 26, 251, 318, 530, 971
BstSFI CTRYAG 1 cut(s) 1020
BstSLI GKGCMC 1 cut(s) 846
BstUI CGCG 2 cut(s) 48, 791
BstV1I GCAGC 3 cut(s) 296, 581, 934
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
Bsu36I CCTNAGG 1 cut(s) 176
BsuRI GGCC 1 cut(s) 318
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 2 cut(s) 102, 913
BtsI GCAGTG 2 cut(s) 273, 501
BtsIMutI CAGTG 3 cut(s) 273, 501, 1083
BtuMI TCGCGA 2 cut(s) 48, 791
BveI ACCTGC 1 cut(s) 593
Cac8I GCNNGC 3 cut(s) 307, 715, 999
CciI TCATGA 2 cut(s) 462, 1156
CfoI GCGC 1 cut(s) 289
Cfr13I GGNCC 3 cut(s) 30, 316, 930
CseI GACGC 1 cut(s) 12
Csp6I GTAC 2 cut(s) 167, 993
CviAII CATG 9 cut(s) 313, 423, 463, 624, 677, 913, 987, 998, 1157
CviQI GTAC 2 cut(s) 167, 993
DdeI CTNAG 3 cut(s) 176, 257, 294
DpnI GATC 5 cut(s) 263, 341, 630, 675, 1058
DpnII GATC 5 cut(s) 261, 339, 628, 673, 1056
DraIII CACNNNGTG 1 cut(s) 850
Ecl136II GAGCTC 1 cut(s) 779
Eco130I CCWWGG 1 cut(s) 1194
Eco24I GRGCYC 1 cut(s) 781
Eco31I GGTCTC 2 cut(s) 754, 1133
Eco47I GGWCC 2 cut(s) 30, 930
Eco53kI GAGCTC 1 cut(s) 779
Eco57I CTGAAG 1 cut(s) 471
Eco81I CCTNAGG 1 cut(s) 176
Eco88I CYCGRG 1 cut(s) 774
EcoICRI GAGCTC 1 cut(s) 779
EcoRI GAATTC 1 cut(s) 109
EcoRII CCWGG 4 cut(s) 251, 318, 530, 971
EcoT14I CCWWGG 1 cut(s) 1194
EcoT22I ATGCAT 1 cut(s) 988
EcoT38I GRGCYC 1 cut(s) 781
ErhI CCWWGG 1 cut(s) 1194
FaeI CATG 9 cut(s) 316, 426, 466, 627, 680, 916, 990, 1001, 1160
FaqI GGGAC 2 cut(s) 10, 943
FatI CATG 9 cut(s) 312, 422, 462, 623, 676, 912, 986, 997, 1156
FauNDI CATATG 1 cut(s) 1145
FbaI TGATCA 1 cut(s) 1056
Fnu4HI GCNGC 4 cut(s) 11, 310, 570, 948
FokI GGATG 2 cut(s) 89, 920
FriOI GRGCYC 1 cut(s) 781
Fsp4HI GCNGC 4 cut(s) 11, 310, 570, 948
GlaI GCGC 1 cut(s) 288
GluI GCNGC 4 cut(s) 11, 310, 570, 948
GsaI CCCAGC 2 cut(s) 930, 1105
HaeIII GGCC 1 cut(s) 318
HapII CCGG 2 cut(s) 28, 270
HgaI GACGC 1 cut(s) 12
HhaI GCGC 1 cut(s) 289
Hin1I GRCGYC 2 cut(s) 5, 835
Hin1II CATG 9 cut(s) 316, 426, 466, 627, 680, 916, 990, 1001, 1160
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HinfI GANTC 5 cut(s) 50, 511, 547, 560, 1126
HpaII CCGG 2 cut(s) 28, 270
HphI GGTGA 5 cut(s) 238, 744, 871, 887, 1066
Hpy188I TCNGA 5 cut(s) 208, 480, 733, 805, 1084
Hpy188III TCNNGA 9 cut(s) 47, 106, 178, 259, 296, 463, 790, 1049, 1157
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 324, 796
HpyCH4III ACNGT 1 cut(s) 1111
HpyCH4IV ACGT 1 cut(s) 835
HpyF10VI GCNNNNNNNGC 4 cut(s) 284, 315, 815, 1173
HpyF3I CTNAG 3 cut(s) 176, 257, 294
HpySE526I ACGT 1 cut(s) 835
Hsp92I GRCGYC 2 cut(s) 5, 835
Hsp92II CATG 9 cut(s) 316, 426, 466, 627, 680, 916, 990, 1001, 1160
HspAI GCGC 1 cut(s) 287
Ksp22I TGATCA 1 cut(s) 1056
Kzo9I GATC 5 cut(s) 261, 339, 628, 673, 1056
LmnI GCTCC 4 cut(s) 18, 451, 814, 1084
Lsp1109I GCAGC 3 cut(s) 296, 581, 934
LweI GCATC 2 cut(s) 460, 796
MaeII ACGT 1 cut(s) 835
MaeIII GTNAC 1 cut(s) 836
MalI GATC 5 cut(s) 263, 341, 630, 675, 1058
MbiI CCGCTC 1 cut(s) 1038
MboI GATC 5 cut(s) 261, 339, 628, 673, 1056
MboII GAAGA 3 cut(s) 197, 623, 1121
MflI RGATCY 1 cut(s) 261
MhlI GDGCHC 2 cut(s) 781, 846
MluCI AATT 7 cut(s) 109, 234, 300, 586, 797, 865, 1090
MlyI GAGTC 2 cut(s) 44, 541
MmeI TCCRAC 2 cut(s) 458, 1062
Mph1103I ATGCAT 1 cut(s) 988
MseI TTAA 1 cut(s) 336
MslI CAYNNNNRTG 3 cut(s) 506, 681, 1076
MspA1I CMGCKG 1 cut(s) 926
MspI CCGG 2 cut(s) 28, 270
MspR9I CCNGG 5 cut(s) 28, 253, 320, 532, 973
MvaI CCWGG 4 cut(s) 253, 320, 532, 973
MvnI CGCG 2 cut(s) 48, 791
MwoI GCNNNNNNNGC 4 cut(s) 284, 315, 815, 1173
NciI CCSGG 1 cut(s) 28
NdeI CATATG 1 cut(s) 1145
NdeII GATC 5 cut(s) 261, 339, 628, 673, 1056
NlaIII CATG 9 cut(s) 316, 426, 466, 627, 680, 916, 990, 1001, 1160
NlaIV GGNNCC 3 cut(s) 195, 931, 970
NmuCI GTSAC 1 cut(s) 836
NruI TCGCGA 2 cut(s) 48, 791
NsiI ATGCAT 1 cut(s) 988
NspI RCATGY 4 cut(s) 426, 627, 990, 1001
OliI CACNNNNGTG 1 cut(s) 506
PaeI GCATGC 1 cut(s) 1001
PaeR7I CTCGAG 1 cut(s) 774
PagI TCATGA 2 cut(s) 462, 1156
PaqCI CACCTGC 1 cut(s) 593
PciI ACATGT 1 cut(s) 422
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PfeI GAWTC 3 cut(s) 511, 560, 1126
PflMI CCANNNNNTGG 1 cut(s) 521
PkrI GCNGC 4 cut(s) 12, 311, 571, 949
PleI GAGTC 2 cut(s) 44, 541
PpsI GAGTC 2 cut(s) 44, 541
PscI ACATGT 1 cut(s) 422
Psp124BI GAGCTC 1 cut(s) 781
Psp6I CCWGG 4 cut(s) 251, 318, 530, 971
PspFI CCCAGC 2 cut(s) 926, 1101
PspGI CCWGG 4 cut(s) 251, 318, 530, 971
PspN4I GGNNCC 3 cut(s) 195, 931, 970
PspPI GGNCC 3 cut(s) 30, 316, 930
PspXI VCTCGAGB 1 cut(s) 774
PsuI RGATCY 1 cut(s) 261
PvuII CAGCTG 1 cut(s) 926
RruI TCGCGA 2 cut(s) 48, 791
RsaI GTAC 2 cut(s) 168, 994
RsaNI GTAC 2 cut(s) 167, 993
RseI CAYNNNNRTG 3 cut(s) 506, 681, 1076
SacI GAGCTC 1 cut(s) 781
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 4 cut(s) 11, 310, 570, 948
Sau3AI GATC 5 cut(s) 261, 339, 628, 673, 1056
Sau96I GGNCC 3 cut(s) 30, 316, 930
SchI GAGTC 2 cut(s) 44, 541
ScrFI CCNGG 5 cut(s) 28, 253, 320, 532, 973
SduI GDGCHC 2 cut(s) 781, 846
SfaNI GCATC 2 cut(s) 460, 796
SfcI CTRYAG 1 cut(s) 1020
Sfr274I CTCGAG 1 cut(s) 774
SinI GGWCC 2 cut(s) 30, 930
SlaI CTCGAG 1 cut(s) 774
SmiMI CAYNNNNRTG 3 cut(s) 506, 681, 1076
SmlI CTYRAG 3 cut(s) 140, 549, 774
SmoI CTYRAG 3 cut(s) 140, 549, 774
SphI GCATGC 1 cut(s) 1001
Sse9I AATT 7 cut(s) 109, 234, 300, 586, 797, 865, 1090
SsiI CCGC 2 cut(s) 1001, 1038
SstI GAGCTC 1 cut(s) 781
StyD4I CCNGG 5 cut(s) 26, 251, 318, 530, 971
StyI CCWWGG 1 cut(s) 1194
TaaI ACNGT 1 cut(s) 1111
TaiI ACGT 1 cut(s) 838
TaqI TCGA 2 cut(s) 775, 1034
TasI AATT 7 cut(s) 109, 234, 300, 586, 797, 865, 1090
TfiI GAWTC 3 cut(s) 511, 560, 1126
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 3 cut(s) 280, 508, 1083
TseFI GTSAC 1 cut(s) 836
TseI GCWGC 4 cut(s) 10, 309, 569, 947
Tsp45I GTSAC 1 cut(s) 836
TspDTI ATGAA 6 cut(s) 246, 453, 623, 878, 1145, 1194
TspGWI ACGGA 1 cut(s) 103
TspRI CASTG 3 cut(s) 280, 508, 1083
Van91I CCANNNNNTGG 1 cut(s) 521
VpaK11BI GGWCC 2 cut(s) 30, 930
XapI RAATTY 3 cut(s) 109, 586, 865
XceI RCATGY 4 cut(s) 426, 627, 990, 1001
XhoI CTCGAG 1 cut(s) 774
ZraI GACGTC 1 cut(s) 836
Zsp2I ATGCAT 1 cut(s) 988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.