Rroxscaffold_4G00312190

Ribose-phosphate pyrophosphokinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0008289
Physical Location & Seq
Reverse (-)
35018069 .. 35028636
10568 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00312190.1

Sequence Viewer

Length: 1194 bp
ATGACTTCGCTGCTTCAGACAGCCTCCCTTTCCTCTCTTTCTTCTTCTCGCTCTCTTCTTCCTAACCCCTCTTCCCGCCCTCGAATCCCGTTCCCTAAAGGCATCAGGTGTAGTGTGGGGGAGCCTTTGAAATACGCGAATGGGAAGCCATGCATTCCACTCCTCAGGGATGAAATGCTGCCGAATTTTCTGACCAGGACTACACATGCGGTGAATGCAGCCAGCAAGAACGACACCAGGCTCAGGATATTTTCCGGAACTGCGAACCCGGCGCTTTCTCAGGAAATTGCATGCTACATGGGTCTGGAACTTGGAAAGATTAAGATAAAACGGTTTGCTGATGGAGAGATATATGTTCAGTTGCAAGAAAGTGTCAGAGGGTGTGATGTATATCTTGTGCAACCAACATGTCCTCCTGCAAATGAGAATCTAATGGAGCTTCTGATAATGATTGATGCATGTCGAAGGGCATCAGCCAAGAACATTACTGCTGTGATTCCATATTTTGGATATGCCAGGGCTGATAGAAAGACTCAAGGGCGTGAATCTATTGCTGCCAAACTTGTTGCAAATTTAATCACTGAAGCGGGTGCAAATCGTGTTCTTGCGTGTGATCTTCATTCTGGTCAATCCATGGGATATTTTGATATTCCTGTGGATCATGTATATGGTCAGCCTGTGATACTTGATTACCTTGCCAGCAAGACTATCTGTTCTGATGATTTGGTAGTGGTCTCACCGGATGTTGGGGGTGTTGCTAGAGCCCGTGCTTTTGCGAAAAAGTTATCTGATGCTCCTCTGGCAATTGTTGATAAAAGACGTCATGGGCACAATGTCGCAGAGGTGATGAATTTGATAGGTGACGTGAAGGGAAAAGTAGCCGTCATGGTTGATGACATGATTGATACAGCTGGGACTATTTCCAAAGGTGCAGAGTTGCTACATCAAGAGGGTGCCAGGGAAGTCTATGCGTGCAGTACACATGCTGTTTTTAGTCCTCCTGCCATAGAGAGGCTGTCAAGCGGTTTGTTCCAAGAGGTGATCATAACAAATACTATTCCAGTGTCTGAGCAGAACTATTTTCCCCAGCTAACTGTCCTGTCAGTGGCAAATCTTCTGGGAGAGACCATATGGCGTGTACATGATGACTGTTCTGGTGGCTTTGAACCCTATTCCACCTTGGGCATTGATTGA

Protein Analysis

397

Amino Acids

43.04

Weight (kDa)

6.71

Isoelectric Point (pI)

41.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pribosyltran_N PF13793 82 - 197 9.5e-53 N-terminal domain of ribose phosphate pyrophosphokinase
Pribosyltran PF00156 241 - 328 3.6e-17 Phosphoribosyl transferase domain
Pribosyl_synth PF14572 282 - 385 1e-22 Phosphoribosyl synthetase-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 823
AccB1I GGYRCC 1 cut(s) 953
AccB7I CCANNNNNTGG 1 cut(s) 506
AccII CGCG 1 cut(s) 137
AccIII TCCGGA 1 cut(s) 254
AciI CCGC 4 cut(s) 76, 209, 587, 1023
AclWI GGATC 1 cut(s) 666
AcsI RAATTY 3 cut(s) 184, 571, 850
AcuI CTGAAG 1 cut(s) 603
AcyI GRCGYC 1 cut(s) 820
AfaI GTAC 2 cut(s) 979, 1140
AfiI CCNNNNNNNGG 5 cut(s) 243, 506, 746, 1011, 1105
AflIII ACRYGT 1 cut(s) 407
AgsI TTSAA 2 cut(s) 130, 1166
AjiI CACGTC 1 cut(s) 865
AjnI CCWGG 4 cut(s) 194, 236, 515, 956
AluBI AGCT 3 cut(s) 439, 911, 1090
AluI AGCT 3 cut(s) 439, 911, 1090
Alw26I GTCTC 2 cut(s) 739, 1118
AlwI GGATC 1 cut(s) 666
AlwNI CAGNNNCTG 1 cut(s) 1067
Aor13HI TCCGGA 1 cut(s) 254
ApeKI GCWGC 4 cut(s) 10, 178, 218, 554
ApoI RAATTY 3 cut(s) 184, 571, 850
AspLEI GCGC 1 cut(s) 274
AsuC2I CCSGG 1 cut(s) 269
AsuHPI GGTGA 5 cut(s) 223, 729, 856, 872, 1051
AxyI CCTNAGG 1 cut(s) 164
BaeGI GKGCMC 1 cut(s) 831
BanI GGYRCC 1 cut(s) 953
BanII GRGCYC 1 cut(s) 766
BbvI GCAGC 3 cut(s) 165, 230, 541
BccI CCATC 1 cut(s) 335
BceAI ACGGC 1 cut(s) 866
BciT130I CCWGG 4 cut(s) 196, 238, 517, 958
BclI TGATCA 1 cut(s) 1041
BcnI CCSGG 1 cut(s) 269
BcoDI GTCTC 2 cut(s) 739, 1118
BfaI CTAG 1 cut(s) 759
BfoI RGCGCY 1 cut(s) 275
BisI GCNGC 4 cut(s) 11, 179, 219, 555
BlsI GCNGC 4 cut(s) 12, 180, 220, 556
Bme1390I CCNGG 5 cut(s) 196, 238, 269, 517, 958
BmgBI CACGTC 1 cut(s) 865
BmiI GGNNCC 2 cut(s) 123, 955
BmrFI CCNGG 5 cut(s) 196, 238, 269, 517, 958
BmsI GCATC 4 cut(s) 111, 445, 479, 781
Bpu10I CCTNAGC 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 519
BpuMI CCSGG 1 cut(s) 269
BsaBI GATNNNNATC 1 cut(s) 390
BsaHI GRCGYC 1 cut(s) 820
BsaI GGTCTC 2 cut(s) 739, 1118
BsaJI CCNNGG 4 cut(s) 516, 633, 957, 1179
BsaWI WCCGGW 2 cut(s) 254, 739
BsaXI ACNNNNNCTCC 2 cut(s) 397, 427
Bsc4I CCNNNNNNNGG 5 cut(s) 243, 506, 746, 1011, 1105
Bse1I ACTGG 1 cut(s) 1061
Bse21I CCTNAGG 1 cut(s) 164
Bse8I GATNNNNATC 1 cut(s) 390
BseAI TCCGGA 1 cut(s) 254
BseBI CCWGG 4 cut(s) 196, 238, 517, 958
BseDI CCNNGG 4 cut(s) 516, 633, 957, 1179
BseGI GGATG 2 cut(s) 175, 748
BseJI GATNNNNATC 1 cut(s) 390
BseLI CCNNNNNNNGG 5 cut(s) 243, 506, 746, 1011, 1105
BseMII CTCAG 4 cut(s) 178, 256, 293, 1059
BseNI ACTGG 1 cut(s) 1061
BseRI GAGGAG 2 cut(s) 152, 786
BseSI GKGCMC 1 cut(s) 831
BseXI GCAGC 3 cut(s) 165, 230, 541
BseYI CCCAGC 2 cut(s) 911, 1086
BsgI GTGCAG 2 cut(s) 951, 994
Bsh1236I CGCG 1 cut(s) 137
BshNI GGYRCC 1 cut(s) 953
BsiSI CCGG 3 cut(s) 255, 269, 740
BslFI GGGAC 1 cut(s) 928
BslI CCNNNNNNNGG 5 cut(s) 243, 506, 746, 1011, 1105
BsmAI GTCTC 2 cut(s) 739, 1118
BsmFI GGGAC 1 cut(s) 928
BsmI GAATGC 2 cut(s) 153, 220
Bso31I GGTCTC 2 cut(s) 739, 1118
Bsp1286I GDGCHC 2 cut(s) 766, 831
Bsp13I TCCGGA 1 cut(s) 254
Bsp1407I TGTACA 1 cut(s) 1138
Bsp143I GATC 3 cut(s) 613, 658, 1041
Bsp19I CCATGG 1 cut(s) 633
BspACI CCGC 4 cut(s) 76, 209, 587, 1023
BspCNI CTCAG 4 cut(s) 177, 255, 292, 1060
BspEI TCCGGA 1 cut(s) 254
BspFNI CGCG 1 cut(s) 137
BspLI GGNNCC 2 cut(s) 123, 955
BspPI GGATC 1 cut(s) 666
BspT107I GGYRCC 1 cut(s) 953
BspTNI GGTCTC 2 cut(s) 739, 1118
BsrGI TGTACA 1 cut(s) 1138
BsrI ACTGG 1 cut(s) 1061
BssECI CCNNGG 4 cut(s) 516, 633, 957, 1179
BssMI GATC 3 cut(s) 613, 658, 1041
BssNI GRCGYC 1 cut(s) 820
BssT1I CCWWGG 2 cut(s) 633, 1179
Bst2UI CCWGG 4 cut(s) 196, 238, 517, 958
Bst4CI ACNGT 3 cut(s) 333, 1096, 1151
Bst6I CTCTTC 2 cut(s) 60, 76
BstACI GRCGYC 1 cut(s) 820
BstAUI TGTACA 1 cut(s) 1138
BstC8I GCNNGC 4 cut(s) 223, 292, 700, 973
BstDEI CTNAG 4 cut(s) 164, 242, 279, 1068
BstDSI CCRYGG 1 cut(s) 633
BstF5I GGATG 2 cut(s) 175, 748
BstFNI CGCG 1 cut(s) 137
BstH2I RGCGCY 1 cut(s) 275
BstHHI GCGC 1 cut(s) 274
BstKTI GATC 3 cut(s) 616, 661, 1044
BstMAI GTCTC 2 cut(s) 739, 1118
BstMBI GATC 3 cut(s) 613, 658, 1041
BstMWI GCNNNNNNNGC 3 cut(s) 215, 269, 800
BstNI CCWGG 4 cut(s) 196, 238, 517, 958
BstNSI RCATGY 5 cut(s) 209, 294, 411, 462, 986
BstSCI CCNGG 5 cut(s) 194, 236, 267, 515, 956
BstSLI GKGCMC 1 cut(s) 831
BstUI CGCG 1 cut(s) 137
BstV1I GCAGC 3 cut(s) 165, 230, 541
Bsu36I CCTNAGG 1 cut(s) 164
BtgI CCRYGG 1 cut(s) 633
BtrI CACGTC 1 cut(s) 865
BtsCI GGATG 2 cut(s) 175, 748
BtsIMutI CAGTG 3 cut(s) 579, 1068, 1110
Cac8I GCNNGC 4 cut(s) 223, 292, 700, 973
CaiI CAGNNNCTG 1 cut(s) 1067
CfoI GCGC 1 cut(s) 274
Csp6I GTAC 2 cut(s) 978, 1139
CviQI GTAC 2 cut(s) 978, 1139
DdeI CTNAG 4 cut(s) 164, 242, 279, 1068
DpnI GATC 3 cut(s) 615, 660, 1043
DpnII GATC 3 cut(s) 613, 658, 1041
Eam1104I CTCTTC 2 cut(s) 60, 76
EarI CTCTTC 2 cut(s) 60, 76
Eco130I CCWWGG 2 cut(s) 633, 1179
Eco24I GRGCYC 1 cut(s) 766
Eco31I GGTCTC 2 cut(s) 739, 1118
Eco57I CTGAAG 1 cut(s) 603
Eco81I CCTNAGG 1 cut(s) 164
EcoRII CCWGG 4 cut(s) 194, 236, 515, 956
EcoT14I CCWWGG 2 cut(s) 633, 1179
EcoT22I ATGCAT 2 cut(s) 155, 460
EcoT38I GRGCYC 1 cut(s) 766
ErhI CCWWGG 2 cut(s) 633, 1179
FaqI GGGAC 1 cut(s) 928
FauI CCCGC 2 cut(s) 83, 580
FauNDI CATATG 1 cut(s) 1130
FbaI TGATCA 1 cut(s) 1041
Fnu4HI GCNGC 4 cut(s) 11, 179, 219, 555
FokI GGATG 2 cut(s) 182, 755
FriOI GRGCYC 1 cut(s) 766
Fsp4HI GCNGC 4 cut(s) 11, 179, 219, 555
FspBI CTAG 1 cut(s) 759
GlaI GCGC 1 cut(s) 273
GluI GCNGC 4 cut(s) 11, 179, 219, 555
GsaI CCCAGC 2 cut(s) 915, 1090
HaeII RGCGCY 1 cut(s) 275
HapII CCGG 3 cut(s) 255, 269, 740
HhaI GCGC 1 cut(s) 274
Hin1I GRCGYC 1 cut(s) 820
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HinfI GANTC 5 cut(s) 84, 427, 496, 532, 545
HpaII CCGG 3 cut(s) 255, 269, 740
HphI GGTGA 5 cut(s) 223, 729, 856, 872, 1051
Hpy166II GTNNAC 2 cut(s) 980, 1139
Hpy188I TCNGA 7 cut(s) 18, 192, 377, 444, 718, 790, 1069
Hpy188III TCNNGA 5 cut(s) 244, 255, 281, 305, 947
Hpy8I GTNNAC 2 cut(s) 980, 1139
HpyAV CCTTC 2 cut(s) 459, 862
HpyCH4III ACNGT 3 cut(s) 333, 1096, 1151
HpyCH4IV ACGT 2 cut(s) 820, 864
HpyF10VI GCNNNNNNNGC 3 cut(s) 215, 269, 800
HpyF3I CTNAG 4 cut(s) 164, 242, 279, 1068
HpySE526I ACGT 2 cut(s) 820, 864
Hsp92I GRCGYC 1 cut(s) 820
HspAI GCGC 1 cut(s) 272
Kpn2I TCCGGA 1 cut(s) 254
Ksp22I TGATCA 1 cut(s) 1041
Kzo9I GATC 3 cut(s) 613, 658, 1041
LmnI GCTCC 3 cut(s) 121, 436, 799
Lsp1109I GCAGC 3 cut(s) 165, 230, 541
LweI GCATC 4 cut(s) 111, 445, 479, 781
MaeI CTAG 1 cut(s) 759
MaeII ACGT 2 cut(s) 820, 864
MaeIII GTNAC 1 cut(s) 860
MalI GATC 3 cut(s) 615, 660, 1043
MboI GATC 3 cut(s) 613, 658, 1041
MboII GAAGA 7 cut(s) 33, 36, 47, 50, 63, 608, 1106
MfeI CAATTG 1 cut(s) 804
MhlI GDGCHC 2 cut(s) 766, 831
MluCI AATT 5 cut(s) 184, 285, 571, 804, 850
MlyI GAGTC 1 cut(s) 526
Mph1103I ATGCAT 2 cut(s) 155, 460
MroI TCCGGA 1 cut(s) 254
MseI TTAA 2 cut(s) 321, 575
MslI CAYNNNNRTG 1 cut(s) 666
MspA1I CMGCKG 1 cut(s) 911
MspI CCGG 3 cut(s) 255, 269, 740
MspR9I CCNGG 5 cut(s) 196, 238, 269, 517, 958
MunI CAATTG 1 cut(s) 804
Mva1269I GAATGC 2 cut(s) 153, 220
MvaI CCWGG 4 cut(s) 196, 238, 517, 958
MvnI CGCG 1 cut(s) 137
MwoI GCNNNNNNNGC 3 cut(s) 215, 269, 800
NciI CCSGG 1 cut(s) 269
NcoI CCATGG 1 cut(s) 633
NdeI CATATG 1 cut(s) 1130
NdeII GATC 3 cut(s) 613, 658, 1041
NlaIV GGNNCC 2 cut(s) 123, 955
NmuCI GTSAC 1 cut(s) 860
NsiI ATGCAT 2 cut(s) 155, 460
NspI RCATGY 5 cut(s) 209, 294, 411, 462, 986
PaeI GCATGC 1 cut(s) 294
PciI ACATGT 1 cut(s) 407
PctI GAATGC 2 cut(s) 153, 220
PfeI GAWTC 4 cut(s) 84, 427, 496, 545
PflMI CCANNNNNTGG 1 cut(s) 506
PkrI GCNGC 4 cut(s) 12, 180, 220, 556
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
PscI ACATGT 1 cut(s) 407
Psp6I CCWGG 4 cut(s) 194, 236, 515, 956
PspFI CCCAGC 2 cut(s) 911, 1086
PspGI CCWGG 4 cut(s) 194, 236, 515, 956
PspN4I GGNNCC 2 cut(s) 123, 955
PstNI CAGNNNCTG 1 cut(s) 1067
PvuII CAGCTG 1 cut(s) 911
RsaI GTAC 2 cut(s) 979, 1140
RsaNI GTAC 2 cut(s) 978, 1139
RseI CAYNNNNRTG 1 cut(s) 666
SaqAI TTAA 2 cut(s) 321, 575
SatI GCNGC 4 cut(s) 11, 179, 219, 555
Sau3AI GATC 3 cut(s) 613, 658, 1041
SchI GAGTC 1 cut(s) 526
ScrFI CCNGG 5 cut(s) 196, 238, 269, 517, 958
SduI GDGCHC 2 cut(s) 766, 831
SfaNI GCATC 4 cut(s) 111, 445, 479, 781
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 1 cut(s) 534
SmoI CTYRAG 1 cut(s) 534
SphI GCATGC 1 cut(s) 294
Sse9I AATT 5 cut(s) 184, 285, 571, 804, 850
SsiI CCGC 4 cut(s) 76, 209, 587, 1023
SspMI CTAG 1 cut(s) 759
StyD4I CCNGG 5 cut(s) 194, 236, 267, 515, 956
StyI CCWWGG 2 cut(s) 633, 1179
TaaI ACNGT 3 cut(s) 333, 1096, 1151
TaiI ACGT 2 cut(s) 823, 867
TaqI TCGA 2 cut(s) 82, 463
TasI AATT 5 cut(s) 184, 285, 571, 804, 850
TatI WGTACW 2 cut(s) 977, 1138
TfiI GAWTC 4 cut(s) 84, 427, 496, 545
Tru1I TTAA 2 cut(s) 321, 575
Tru9I TTAA 2 cut(s) 321, 575
TscAI CASTG 3 cut(s) 586, 1068, 1110
TseFI GTSAC 1 cut(s) 860
TseI GCWGC 4 cut(s) 10, 178, 218, 554
Tsp45I GTSAC 1 cut(s) 860
TspDTI ATGAA 3 cut(s) 186, 608, 863
TspRI CASTG 3 cut(s) 586, 1068, 1110
Van91I CCANNNNNTGG 1 cut(s) 506
XapI RAATTY 3 cut(s) 184, 571, 850
XceI RCATGY 5 cut(s) 209, 294, 411, 462, 986
XspI CTAG 1 cut(s) 759
ZraI GACGTC 1 cut(s) 821
Zsp2I ATGCAT 2 cut(s) 155, 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.