AT3G02060

CarD-like/TRCF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0015231
Physical Location & Seq
Forward (+)
354360 .. 358833
4474 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G02060.1

Sequence Viewer

Length: 2472 bp
ATGATGTCTTTGCTCCCAAATCCCGACCCGATTACTGTACCCCTGGTCCTCAAGCTTTGCTCCTTTCCTCCGCCACGGAGGCTCTTCTCTCTCAGGCTCAGGCGCTTCACCCGAAAGTCATCATCTCTTCTTCCGTTGGTCGCTGTTTCGTCTCTCTCCGCTACTGCCGCAAAACCTACCAGATGGAGAGAGAAGCCGGAATTGGCGGAAAGCGACTCAATTTCCCTCCTCAACGAGAGGATTCGGCGTGACCTCGGCAAGAGAGAGACTGCTAGACCGGCCATGGACTCTGAGGAGGCCGAGAAGTACATTCACATGGTCAAGGAACAACAAGAGAGGGGTCTGCAGAAGCTCAAAGGAATTAGGCAAGGTACAAAGGCTGCTGGTGACGGTGCTTTTAGTTACAAGGTTGACCCTTACAGTCTCCTTTCCGGTGATTATGTGGTGCACAAGAAAGTAGGCATTGGGCGTTTTGTTGGGATCAAGTTTGATGTCCCCAAGGACTCCTCTGAGCCCCTCGAATATGTCTTTATAGAGTATGCTGATGGTATGGCCAAGCTTCCCCTCAAACAGGCCTCGCGTTTGCTCTACCGATACAATCTTCCAAATGAGACTAAACGGCCTCGGACTTTGAGTCGGCTGAGTGACACTAGTGTTTGGGAAAGAAGAAAGACCAAAGGAAAAGTAGCAATTCAGAAAATGGTCGTTGACTTGATGGAGCTATATCTTCATAGGCTTAGACAGAAGAGATATCCATATCCGAAGAACCCCATCATGGCTGATTTTGCGGCTCAATTTCCTTATAACGCCACTCCTGACCAGAAGCAGGCTTTCCTGGATGTTGAAAAGGATTTGACTGAGAGAGAAACACCTATGGACCGATTGATCTGTGGAGATGTTGGCTTTGGTAAAACAGAGGTTGCTCTACGAGCCATCTTTTGTGTGGTCTCAACTGGAAAACAAGCTATGGTTTTAGCACCGACAATTGTATTGGCGAAGCAACATTACGATGTAATCTCAGAGCGGTTTTCCTTGTATCCACACATCAAAGTGGGCCTTTTAAGTCGGTTTCAGACCAAAGCTGAAAAGGAGGAGTATTTGGAAATGATAAAAACTGGACATCTCAATATCATTGTGGGTACTCACTCACTTCTCGGGAGCCGTGTTGTGTACAGTAATTTAGGCCTCCTTGTGGTTGATGAGGAGCAGAGATTTGGGGTCAAGCAGAAAGAAAAGATTGCATCTTTCAAAACATCCGTGGATGTGCTTACATTGTCCGCAACACCCATACCAAGGACACTGTACTTAGCTTTGACTGGATTCCGGGATGCTAGTTTAATCTCCACACCGCCTCCGGAGAGGATTCCAATAAAAACCCATCTTTCGTCATTCCGTAAGGAAAAGGTGATTGAAGCAATAAAAAATGAGCTGGATCGTGGTGGCCAAGTTTTTTATGTCTTGCCTCGGATTAAAGGACTAGAAGAAGTGATGGATTTTCTTGAAGAAGCATTTCCAGATATTGACATTGCTATGGCACATGGAAAGCAATACTCGAAACAACTAGAGGAAACCATGGAGAGATTTGCGCAAGGAAAGATCAAAATCCTCATATGTACTAATATTGTTGAAAGCGGACTTGATATTCAAAATGCAAATACCATAATCATTCAGGATGTTCAACAATTTGGGCTCGCTCAGTTGTACCAGTTGCGTGGAAGGGTTGGTCGGGCAGATAAAGAAGCTCATGCCTACCTATTTTATCCCGATAAATCGCTGCTCTCTGATCAAGCACTGGAAAGGCTTAGCGCTCTTGAAGAGTGCCGTGAACTTGGACAAGGCTTCCAACTTGCAGAGAAGGATATGGGTATAAGAGGTTTTGGGACAATCTTTGGAGAACAACAGACGGGGGATGTTGGAAATGTCGGTATCGATCTCTTCTTTGAAATGCTTTTTGAGAGTCTCTCCAAGGTGGAGGAACTCCGTATTTTTTCGGTTCCATATGACCTCGTGAAGATTGACATAAATATAAATCCCCGGCTACCCTCGGAGTATGTAAATTACCTGGAAAATCCGATGGAGATCATTCATGAAGCTGAAAAAGCAGCAGAGAAAGATATGTGGAGTCTAATGCAATTTACAGAGAACCTGCGTCGCCAATATGGGAAAGAGCCTTACTCCATGGAAATCATTTTAAAGAAGCTGTATGTGAGACGCATGGCGGCTGATCTTGGAGTAAACAGAATTTATGCATCAGGCAAGATGGTTGTCATGAAAACAAATATGAGTAAGAAGGTGTTCAAGCTGATCACAGATTCCATGACTTGTGACGTTTACCGAAGCTCCTTGATATATGAAGGAGATCAAATAATGGCGGAACTTCTGTTGGAGCTACCAAGAGAACAGTTACTGAACTGGATGTTCCAGTGCTTGTCAGAACTTCATGCATCACTTCCTGCTCTTATCAAATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarD_CdnL_TRCF DEAD Helicase_C TRCF

Protein Analysis

823

Amino Acids

93.93

Weight (kDa)

8.9

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CarD_TRCF_RID PF02559 144 - 199 2.5e-09 CarD-like/TRCF RID domain
ResIII PF04851 270 - 429 1.7e-08 Type III restriction enzyme, res subunit
DEAD PF00270 271 - 433 2.1e-23 DEAD/DEAH box helicase
Helicase_C PF00271 470 - 577 1.6e-21 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02060 AT3G02060 AT3G02060
fragaria_vesca FvH4_6g54430
malus_domestica MD17G1004500.v1.1
prunus_persica Prupe.3G314900_v2.0.a1
pyrus_communis pycom111g00110 pycom17g00110
rosa_chinensis RchiOBHm_Chr2g0176681
rosa_laevigata RLG00000022435
rosa_rugosa Rorug02G0597500
rosa_samantha Rh2BG686000 Rh2CG649300 Rh2DG700200
rosa_wichuraiana Rw0G015680 Rw2G055390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 804
Acc16I TGCGCA 1 cut(s) 1587
Acc36I ACCTGC 1 cut(s) 2154
AccBSI CCGCTC 1 cut(s) 1024
AccII CGCG 1 cut(s) 580
AccIII TCCGGA 1 cut(s) 1354
AclWI GGATC 2 cut(s) 488, 1440
AcoI YGGCCR 3 cut(s) 279, 552, 1441
AcsI RAATTY 1 cut(s) 2241
AfaI GTAC 8 cut(s) 39, 308, 373, 1141, 1172, 1304, 1615, 1703
AfeI AGCGCT 1 cut(s) 1807
AfiI CCNNNNNNNGG 5 cut(s) 571, 775, 826, 1192, 1293
AhdI GACNNNNNGTC 1 cut(s) 633
AhlI ACTAGT 1 cut(s) 650
AjnI CCWGG 3 cut(s) 42, 834, 2061
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 7 cut(s) 156, 260, 428, 605, 952, 1964, 2203
Alw44I GTGCAC 1 cut(s) 446
AlwI GGATC 2 cut(s) 488, 1440
AlwNI CAGNNNCTG 1 cut(s) 2407
Ama87I CYCGRG 1 cut(s) 1154
Aor13HI TCCGGA 1 cut(s) 1354
Aor51HI AGCGCT 1 cut(s) 1807
AoxI GGCC 8 cut(s) 279, 297, 552, 573, 620, 1054, 1183, 1441
ApaLI GTGCAC 1 cut(s) 446
ApeKI GCWGC 3 cut(s) 380, 1774, 2102
ApoI RAATTY 1 cut(s) 2241
Asp700I GAANNNNTTC 2 cut(s) 1509, 2294
AspLEI GCGC 3 cut(s) 105, 1588, 1808
AspS9I GGNCC 3 cut(s) 46, 877, 1054
AsuC2I CCSGG 2 cut(s) 1325, 2035
AsuHPI GGTGA 4 cut(s) 100, 398, 446, 1417
AvaI CYCGRG 1 cut(s) 1154
AvaII GGWCC 2 cut(s) 46, 877
BaeGI GKGCMC 1 cut(s) 450
BalI TGGCCA 2 cut(s) 554, 1443
BanII GRGCYC 2 cut(s) 516, 1692
BarI GAAGNNNNNNTAC 2 cut(s) 989, 1021
BauI CACGAG 1 cut(s) 2006
Bbv12I GWGCWC 1 cut(s) 450
BbvI GCAGC 3 cut(s) 367, 1761, 2114
BccI CCATC 9 cut(s) 177, 539, 709, 779, 941, 1386, 1483, 2068, 2254
BceAI ACGGC 3 cut(s) 635, 1146, 1806
BciT130I CCWGG 3 cut(s) 44, 836, 2063
BciVI GTATCC 1 cut(s) 1047
BclI TGATCA 2 cut(s) 1783, 2304
BcnI CCSGG 2 cut(s) 1325, 2035
BcoDI GTCTC 7 cut(s) 156, 260, 428, 605, 952, 1964, 2203
BcuI ACTAGT 1 cut(s) 650
BfaI CTAG 5 cut(s) 273, 651, 1332, 1478, 1562
BfmI CTRYAG 1 cut(s) 344
BfoI RGCGCY 2 cut(s) 106, 1809
BfuAI ACCTGC 1 cut(s) 2154
BfuI GTATCC 1 cut(s) 1047
BglI GCCNNNNNGGC 1 cut(s) 79
BisI GCNGC 6 cut(s) 168, 381, 789, 1775, 2103, 2220
BlpI GCTNAGC 1 cut(s) 1802
BlsI GCNGC 6 cut(s) 169, 382, 790, 1776, 2104, 2221
Bme1390I CCNGG 5 cut(s) 44, 836, 1325, 2035, 2063
Bme18I GGWCC 2 cut(s) 46, 877
BmeRI GACNNNNNGTC 1 cut(s) 633
BmeT110I CYCGRG 1 cut(s) 1154
BmgT120I GGNCC 3 cut(s) 46, 877, 1054
BmiI GGNNCC 2 cut(s) 1160, 1995
BmrFI CCNGG 5 cut(s) 44, 836, 1325, 2035, 2063
BmsI GCATC 4 cut(s) 1250, 1318, 2258, 2453
BplI GAGNNNNNCTC 4 cut(s) 1946, 1978, 2159, 2191
Bpu10I CCTNAGC 1 cut(s) 98
Bpu1102I GCTNAGC 1 cut(s) 1802
BpuEI CTTGAG 1 cut(s) 35
BpuMI CCSGG 2 cut(s) 1325, 2035
Bsa29I ATCGAT 1 cut(s) 1929
BsaBI GATNNNNATC 2 cut(s) 1601, 2078
BsaI GGTCTC 1 cut(s) 952
BsaWI WCCGGW 2 cut(s) 431, 1354
BsaXI ACNNNNNCTCC 4 cut(s) 1336, 1366, 2324, 2354
Bsc4I CCNNNNNNNGG 5 cut(s) 571, 775, 826, 1192, 1293
Bse118I RCCGGY 1 cut(s) 277
Bse1I ACTGG 7 cut(s) 958, 1120, 1321, 1705, 1797, 2417, 2422
Bse3DI GCAATG 1 cut(s) 1524
Bse8I GATNNNNATC 2 cut(s) 1601, 2078
BseAI TCCGGA 1 cut(s) 1354
BseBI CCWGG 3 cut(s) 44, 836, 2063
BseCI ATCGAT 1 cut(s) 1929
BseGI GGATG 7 cut(s) 844, 1253, 1267, 1333, 1678, 1915, 2421
BseJI GATNNNNATC 2 cut(s) 1601, 2078
BseLI CCNNNNNNNGG 5 cut(s) 571, 775, 826, 1192, 1293
BseMI GCAATG 1 cut(s) 1524
BseMII CTCAG 8 cut(s) 106, 112, 282, 501, 632, 849, 1032, 1709
BseNI ACTGG 7 cut(s) 958, 1120, 1321, 1705, 1797, 2417, 2422
BseRI GAGGAG 5 cut(s) 218, 308, 496, 1106, 1217
BseSI GKGCMC 1 cut(s) 450
BseXI GCAGC 3 cut(s) 367, 1761, 2114
Bsh1236I CGCG 1 cut(s) 580
BshFI GGCC 8 cut(s) 281, 299, 554, 575, 622, 1056, 1185, 1443
BshVI ATCGAT 1 cut(s) 1929
BsiHKAI GWGCWC 1 cut(s) 450
BsiHKCI CYCGRG 1 cut(s) 1154
BsiSI CCGG 6 cut(s) 197, 278, 432, 1324, 1355, 2035
BslFI GGGAC 2 cut(s) 479, 1894
BslI CCNNNNNNNGG 5 cut(s) 571, 775, 826, 1192, 1293
BsmAI GTCTC 7 cut(s) 156, 260, 428, 605, 952, 1964, 2203
BsmBI CGTCTC 2 cut(s) 156, 2203
BsmFI GGGAC 2 cut(s) 479, 1894
BsnI GGCC 8 cut(s) 281, 299, 554, 575, 622, 1056, 1185, 1443
Bso31I GGTCTC 1 cut(s) 952
BsoBI CYCGRG 1 cut(s) 1154
Bsp1286I GDGCHC 3 cut(s) 450, 516, 1692
Bsp13I TCCGGA 1 cut(s) 1354
Bsp1407I TGTACA 1 cut(s) 1170
Bsp1720I GCTNAGC 1 cut(s) 1802
Bsp19I CCATGG 3 cut(s) 282, 1572, 2178
BspANI GGCC 8 cut(s) 281, 299, 554, 575, 622, 1056, 1185, 1443
BspCNI CTCAG 8 cut(s) 105, 111, 283, 502, 633, 850, 1031, 1708
BspDI ATCGAT 1 cut(s) 1929
BspEI TCCGGA 1 cut(s) 1354
BspFNI CGCG 1 cut(s) 580
BspHI TCATGA 2 cut(s) 2086, 2268
BspLI GGNNCC 2 cut(s) 1160, 1995
BspMAI CTGCAG 1 cut(s) 348
BspMI ACCTGC 1 cut(s) 2154
BspPI GGATC 2 cut(s) 488, 1440
BspQI GCTCTTC 1 cut(s) 89
BspTNI GGTCTC 1 cut(s) 952
BsrBI CCGCTC 1 cut(s) 1024
BsrDI GCAATG 1 cut(s) 1524
BsrFI RCCGGY 1 cut(s) 277
BsrGI TGTACA 1 cut(s) 1170
BsrI ACTGG 7 cut(s) 958, 1120, 1321, 1705, 1797, 2417, 2422
BssAI RCCGGY 1 cut(s) 277
BssSI CACGAG 1 cut(s) 2006
BssT1I CCWWGG 6 cut(s) 282, 498, 1292, 1572, 1965, 2178
Bst2BI CACGAG 1 cut(s) 2006
Bst2UI CCWGG 3 cut(s) 44, 836, 2063
Bst4CI ACNGT 6 cut(s) 37, 392, 422, 1175, 1302, 2403
Bst6I CTCTTC 5 cut(s) 89, 132, 740, 1809, 1940
BstAUI TGTACA 1 cut(s) 1170
BstC8I GCNNGC 2 cut(s) 828, 1692
BstDSI CCRYGG 5 cut(s) 74, 282, 1257, 1572, 2178
BstENI CCTNNNNNAGG 1 cut(s) 569
BstF5I GGATG 7 cut(s) 844, 1253, 1267, 1333, 1678, 1915, 2421
BstFNI CGCG 1 cut(s) 580
BstH2I RGCGCY 2 cut(s) 106, 1809
BstHHI GCGC 3 cut(s) 105, 1588, 1808
BstMAI GTCTC 7 cut(s) 156, 260, 428, 605, 952, 1964, 2203
BstMWI GCNNNNNNNGC 6 cut(s) 79, 167, 278, 785, 929, 2099
BstNI CCWGG 3 cut(s) 44, 836, 2063
BstSCI CCNGG 5 cut(s) 42, 834, 1323, 2033, 2061
BstSFI CTRYAG 1 cut(s) 344
BstSLI GKGCMC 1 cut(s) 450
BstUI CGCG 1 cut(s) 580
BstV1I GCAGC 3 cut(s) 367, 1761, 2114
BstXI CCANNNNNNTGG 1 cut(s) 1712
Bsu15I ATCGAT 1 cut(s) 1929
BsuI GTATCC 1 cut(s) 1047
BsuRI GGCC 8 cut(s) 281, 299, 554, 575, 622, 1056, 1185, 1443
BsuTUI ATCGAT 1 cut(s) 1929
BtgI CCRYGG 5 cut(s) 74, 282, 1257, 1572, 2178
BtsCI GGATG 7 cut(s) 844, 1253, 1267, 1333, 1678, 1915, 2421
BtsIMutI CAGTG 3 cut(s) 1298, 1790, 2429
BveI ACCTGC 1 cut(s) 2154
Cac8I GCNNGC 2 cut(s) 828, 1692
CaiI CAGNNNCTG 1 cut(s) 2407
CciI TCATGA 2 cut(s) 2086, 2268
CfoI GCGC 3 cut(s) 105, 1588, 1808
Cfr10I RCCGGY 1 cut(s) 277
Cfr13I GGNCC 3 cut(s) 46, 877, 1054
ClaI ATCGAT 1 cut(s) 1929
CseI GACGC 2 cut(s) 2138, 2220
Csp6I GTAC 8 cut(s) 38, 307, 372, 1140, 1171, 1303, 1614, 1702
CviQI GTAC 8 cut(s) 38, 307, 372, 1140, 1171, 1303, 1614, 1702
DraI TTTAAA 1 cut(s) 2193
DriI GACNNNNNGTC 1 cut(s) 633
EaeI YGGCCR 3 cut(s) 279, 552, 1441
Eam1104I CTCTTC 5 cut(s) 89, 132, 740, 1809, 1940
Eam1105I GACNNNNNGTC 1 cut(s) 633
EarI CTCTTC 5 cut(s) 89, 132, 740, 1809, 1940
EciI GGCGGA 3 cut(s) 60, 221, 2387
Eco130I CCWWGG 6 cut(s) 282, 498, 1292, 1572, 1965, 2178
Eco147I AGGCCT 2 cut(s) 575, 1185
Eco24I GRGCYC 2 cut(s) 516, 1692
Eco31I GGTCTC 1 cut(s) 952
Eco32I GATATC 1 cut(s) 752
Eco47I GGWCC 2 cut(s) 46, 877
Eco47III AGCGCT 1 cut(s) 1807
Eco88I CYCGRG 1 cut(s) 1154
EcoNI CCTNNNNNAGG 1 cut(s) 569
EcoRII CCWGG 3 cut(s) 42, 834, 2061
EcoRV GATATC 1 cut(s) 752
EcoT14I CCWWGG 6 cut(s) 282, 498, 1292, 1572, 1965, 2178
EcoT22I ATGCAT 2 cut(s) 2251, 2446
EcoT38I GRGCYC 2 cut(s) 516, 1692
ErhI CCWWGG 6 cut(s) 282, 498, 1292, 1572, 1965, 2178
Esp3I CGTCTC 2 cut(s) 156, 2203
FalI AAGNNNNNCTT 2 cut(s) 1041, 1073
FaqI GGGAC 2 cut(s) 479, 1894
FauNDI CATATG 2 cut(s) 1610, 1999
FbaI TGATCA 2 cut(s) 1783, 2304
Fnu4HI GCNGC 6 cut(s) 168, 381, 789, 1775, 2103, 2220
FokI GGATG 7 cut(s) 851, 1240, 1274, 1340, 1685, 1922, 2428
FriOI GRGCYC 2 cut(s) 516, 1692
Fsp4HI GCNGC 6 cut(s) 168, 381, 789, 1775, 2103, 2220
FspBI CTAG 5 cut(s) 273, 651, 1332, 1478, 1562
FspI TGCGCA 1 cut(s) 1587
GlaI GCGC 3 cut(s) 104, 1587, 1807
GluI GCNGC 6 cut(s) 168, 381, 789, 1775, 2103, 2220
HaeII RGCGCY 2 cut(s) 106, 1809
HaeIII GGCC 8 cut(s) 281, 299, 554, 575, 622, 1056, 1185, 1443
HapII CCGG 6 cut(s) 197, 278, 432, 1324, 1355, 2035
HgaI GACGC 2 cut(s) 2138, 2220
HhaI GCGC 3 cut(s) 105, 1588, 1808
Hin6I GCGC 3 cut(s) 103, 1586, 1806
HinP1I GCGC 3 cut(s) 103, 1586, 1806
HincII GTYRAC 2 cut(s) 412, 709
HindII GTYRAC 2 cut(s) 412, 709
HindIII AAGCTT 2 cut(s) 53, 557
HpaII CCGG 6 cut(s) 197, 278, 432, 1324, 1355, 2035
HphI GGTGA 4 cut(s) 100, 398, 446, 1417
Hpy166II GTNNAC 7 cut(s) 412, 448, 709, 1171, 1826, 2236, 2332
Hpy8I GTNNAC 7 cut(s) 412, 448, 709, 1171, 1826, 2236, 2332
Hpy99I CGWCG 1 cut(s) 2154
HpyAV CCTTC 4 cut(s) 1710, 1849, 2284, 2348
HpyCH4III ACNGT 6 cut(s) 37, 392, 422, 1175, 1302, 2403
HpyCH4IV ACGT 1 cut(s) 2328
HpyCH4V TGCA 8 cut(s) 346, 448, 1241, 1652, 1850, 2131, 2249, 2444
HpyF10VI GCNNNNNNNGC 6 cut(s) 79, 167, 278, 785, 929, 2099
HpySE526I ACGT 1 cut(s) 2328
HspAI GCGC 3 cut(s) 103, 1586, 1806
Kpn2I TCCGGA 1 cut(s) 1354
Ksp22I TGATCA 2 cut(s) 1783, 2304
LguI GCTCTTC 1 cut(s) 89
LmnI GCTCC 7 cut(s) 18, 65, 718, 1158, 1204, 2345, 2386
Lsp1109I GCAGC 3 cut(s) 367, 1761, 2114
LweI GCATC 4 cut(s) 1250, 1318, 2258, 2453
MaeI CTAG 5 cut(s) 273, 651, 1332, 1478, 1562
MaeII ACGT 1 cut(s) 2328
MaeIII GTNAC 6 cut(s) 248, 386, 401, 644, 2324, 2403
MbiI CCGCTC 1 cut(s) 1024
MfeI CAATTG 1 cut(s) 984
MhlI GDGCHC 3 cut(s) 450, 516, 1692
MlsI TGGCCA 2 cut(s) 554, 1443
MluNI TGGCCA 2 cut(s) 554, 1443
MlyI GAGTC 6 cut(s) 209, 281, 497, 643, 1966, 2131
MmeI TCCRAC 3 cut(s) 1867, 1894, 2364
Mox20I TGGCCA 2 cut(s) 554, 1443
Mph1103I ATGCAT 2 cut(s) 2251, 2446
MroI TCCGGA 1 cut(s) 1354
MroXI GAANNNNTTC 2 cut(s) 1509, 2294
MscI TGGCCA 2 cut(s) 554, 1443
MseI TTAA 4 cut(s) 1061, 1337, 1470, 2192
MslI CAYNNNNRTG 4 cut(s) 314, 1008, 1049, 1529
Msp20I TGGCCA 2 cut(s) 554, 1443
MspI CCGG 6 cut(s) 197, 278, 432, 1324, 1355, 2035
MspR9I CCNGG 5 cut(s) 44, 836, 1325, 2035, 2063
MunI CAATTG 1 cut(s) 984
MvaI CCWGG 3 cut(s) 44, 836, 2063
MvnI CGCG 1 cut(s) 580
MwoI GCNNNNNNNGC 6 cut(s) 79, 167, 278, 785, 929, 2099
NciI CCSGG 2 cut(s) 1325, 2035
NcoI CCATGG 3 cut(s) 282, 1572, 2178
NdeI CATATG 2 cut(s) 1610, 1999
NlaIV GGNNCC 2 cut(s) 1160, 1995
NmeAIII GCCGAG 2 cut(s) 234, 325
NmuCI GTSAC 4 cut(s) 248, 386, 644, 2324
NsbI TGCGCA 1 cut(s) 1587
NsiI ATGCAT 2 cut(s) 2251, 2446
PagI TCATGA 2 cut(s) 2086, 2268
PceI AGGCCT 2 cut(s) 575, 1185
PciSI GCTCTTC 1 cut(s) 89
PdmI GAANNNNTTC 2 cut(s) 1509, 2294
PfeI GAWTC 4 cut(s) 241, 1320, 1363, 2312
PfoI TCCNGGA 2 cut(s) 834, 1323
PkrI GCNGC 6 cut(s) 169, 382, 790, 1776, 2104, 2221
PleI GAGTC 6 cut(s) 209, 281, 497, 642, 1965, 2130
PpsI GAGTC 6 cut(s) 209, 281, 497, 642, 1965, 2130
PsiI TTATAA 1 cut(s) 804
Psp6I CCWGG 3 cut(s) 42, 834, 2061
PspGI CCWGG 3 cut(s) 42, 834, 2061
PspN4I GGNNCC 2 cut(s) 1160, 1995
PspPI GGNCC 3 cut(s) 46, 877, 1054
PstI CTGCAG 1 cut(s) 348
PstNI CAGNNNCTG 1 cut(s) 2407
RsaI GTAC 8 cut(s) 39, 308, 373, 1141, 1172, 1304, 1615, 1703
RsaNI GTAC 8 cut(s) 38, 307, 372, 1140, 1171, 1303, 1614, 1702
RseI CAYNNNNRTG 4 cut(s) 314, 1008, 1049, 1529
SapI GCTCTTC 1 cut(s) 89
SaqAI TTAA 4 cut(s) 1061, 1337, 1470, 2192
SatI GCNGC 6 cut(s) 168, 381, 789, 1775, 2103, 2220
Sau96I GGNCC 3 cut(s) 46, 877, 1054
SchI GAGTC 6 cut(s) 209, 281, 497, 643, 1966, 2131
ScrFI CCNGG 5 cut(s) 44, 836, 1325, 2035, 2063
SduI GDGCHC 3 cut(s) 450, 516, 1692
SfaNI GCATC 4 cut(s) 1250, 1318, 2258, 2453
SfcI CTRYAG 1 cut(s) 344
SinI GGWCC 2 cut(s) 46, 877
SmiMI CAYNNNNRTG 4 cut(s) 314, 1008, 1049, 1529
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
SpeI ACTAGT 1 cut(s) 650
SseBI AGGCCT 2 cut(s) 575, 1185
SspI AATATT 2 cut(s) 1621, 2468
SspMI CTAG 5 cut(s) 273, 651, 1332, 1478, 1562
StuI AGGCCT 2 cut(s) 575, 1185
StyD4I CCNGG 5 cut(s) 42, 834, 1323, 2033, 2061
StyI CCWWGG 6 cut(s) 282, 498, 1292, 1572, 1965, 2178
TaaI ACNGT 6 cut(s) 37, 392, 422, 1175, 1302, 2403
TaiI ACGT 1 cut(s) 2331
TaqI TCGA 3 cut(s) 519, 1553, 1929
TaqII GACCGA 1 cut(s) 894
TatI WGTACW 4 cut(s) 306, 1170, 1302, 1613
TauI GCSGC 3 cut(s) 170, 791, 2222
TfiI GAWTC 4 cut(s) 241, 1320, 1363, 2312
Tru1I TTAA 4 cut(s) 1061, 1337, 1470, 2192
Tru9I TTAA 4 cut(s) 1061, 1337, 1470, 2192
TscAI CASTG 3 cut(s) 1305, 1797, 2429
TseFI GTSAC 4 cut(s) 248, 386, 644, 2324
TseI GCWGC 3 cut(s) 380, 1774, 2102
Tsp45I GTSAC 4 cut(s) 248, 386, 644, 2324
TspDTI ATGAA 6 cut(s) 719, 2075, 2103, 2285, 2367, 2429
TspGWI ACGGA 5 cut(s) 91, 123, 1246, 1382, 1970
TspRI CASTG 3 cut(s) 1305, 1797, 2429
VneI GTGCAC 1 cut(s) 446
VpaK11BI GGWCC 2 cut(s) 46, 877
XagI CCTNNNNNAGG 1 cut(s) 569
XapI RAATTY 1 cut(s) 2241
XcmI CCANNNNNNNNNTGG 1 cut(s) 940
XmnI GAANNNNTTC 2 cut(s) 1509, 2294
XspI CTAG 5 cut(s) 273, 651, 1332, 1478, 1562
Zsp2I ATGCAT 2 cut(s) 2251, 2446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.