Rh2BG686000

CarD-like/TRCF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0015231
Physical Location & Seq
Forward (+)
89360219 .. 89365822
5604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG686000.1

Sequence Viewer

Length: 2481 bp
ATGGCTCTGCTCCTCCCCATCCCAATCCCAATCCATGGCGTTTCTTCTTCTTCTTCTTCTTCTTGTTATAGGTCGAGGTCGGCGAGTCGGCTGTGCAGGGTTTGGGGTTGGGGACTGTTGAAAATCCAGAGAGGGGGCCGCACCCAGACGCCGACGCCGACGCCGACGAAGAACAGCCTGGGCCTTTCCAATTCCACTTTCACGAAAACCAAATCGAGCAAAAAGCGACGGGACGCGGCTGTGGAGGCAGAGTCGGACGCCATATCGATTCTAAACGAGAGAATTCGGCGCGAGCAGAGAAAGAAATCGTCGGAGGGGTCTCCGATGGAGGCGGAGGAGGCAGACAAGTACTTGGAGCTGGTGAAGCAGCAGCAGCAGAGGGGCCTCCAGAAGCTCAAAGGAGACCTTAGTTACAAGGTCGACCCCTACACTCTTCGTTCCGGTGATTATGTGGTCCATAAGAAGGTCGGCCTCGGTCGATTCGTTGGGATTAAATTCGACAATGCTCCTGCTTCCATGGACCCTATTGAATACGTCTTCATTGAGTATGCTGATGGCATGGCTAAGCTTCCTGTTAAGCAGGCCTCTCGCTTGCTCTATCGTTACAATCTCCCCAACGAAAACAAAAAACCTCACACATTGAGCAAACTGAACGACACCAGTGTGTGGGAGAGAAGAAAGACCAAGGGAAAGATTGCCATTCAGAAAATGGTTGTTGATCTGATGGAGTTGTATTTACACAGGCTCAAACAAACACGCCCTCCCTACCCTCTTACTCCTGCCATGGCTCACTTTGTTTCTCAGTTTCCGTATGAGCCTACTCCTGATCAAAAGCAGGCTTTCATTGATGTTGAGAAAGATTTAACAGGGAGGGAAACTCCAATGGACCGCTTAATTTGTGGAGACGTTGGCTTTGGCAAAACTGAAGTTGCACTCCGTGCCATCTTTTGTGTAGTTTCCGCAGGGAAGCAAGCCATGGTTCTTGCACCCACAATTGTTTTAGCCAAGCAACATTTTGAAGTTATCTCACAGCGATTTTCTATCTACCCAAATATCAACGTTGGACTTCTTAGCAGGTTTCAGACTAAATCAGAGAAAGATGAGCATCTAGACATGATAAAGAATGGTCATTTGGACATAATTGTTGGGACACACTCACTTCTTGGAAGTCGTGTTGTGTATAATAATCTAGGCCTGCTTGTCGTTGATGAAGAACAGAGGTTTGGCGTCAAACAGAAAGAGAAGATTGCTTCATTCAAAACATCAGTTGATGTTCTTACTCTATCTGCAACACCTATACCGCGAACCCTTTATTTAGCATTGACTGGTTTTCGTGATGCTAGCTTGATTTCAACTCCACCTCCAGAAAGAGTCCCTATAAGAACCCATCTCTCGGCATACAGTAAAGAAAAGGTATTATCAGCAATTAAGTATGAGCTGGAACGAGGTGGCCAAGTATTTTATGTTTTGCCTCGTATCAAAGGACTTGAAGAAGTGATGAGTTTTCTTGAACAGTCGTTTCCTGATGTTGAAATAGCTATTGCACACGGGAAGCAATATTCAAAGCAGCTAGAGGAAACCATGGAGAAATTTTCACAAGGTGAAATCAAGATTCTTACATGCACTAATATAGTAGAAAGTGGGCTTGACATTCAGAATGCAAACACCATTATAATTCAGGATGTTCAACAATTTGGCCTAGCACAGTTGTATCAGCTGCGAGGAAGGGTGGGGAGGGCAGATAAGGAAGCTCACGCACACTTATTTTATCCAGAGAAGTCACTTCTAACTGATCAGGCGTTGGAGAGACTTGCAGCTCTAGAGGAATGCTGTCAACTTGGGCAAGGTTTCCAACTTGCAGAGAGGGACATGGGAATAAGAGGCTTTGGTACCATCTTTGGTGAGCAGCAGACTGGGGATGTTGGAAATGTTGGCATTGATCTCTTCTTTGAAATGCTGTTTGAGAGCTTGTCTAAGGTTGATGAGCATCGAGTAGTTTCAGTTCCTTACTGGTCCGTTGAGATTGATATGAATATAAATCCTCATCTCCCTTCTGAGTACATAAATAATCTCGAGAATCCCATGGAGATAATTCATGAAGCTGAGAAAGCTGCAGAGAAGGATATCTGGAGTTTGATGCAATATACTGAGAATTTGCGGCGTCAATATGGAAAGGAGCCACACTCCATGGAAATTTTATTAAAGAAGCTTTATGTGAGGAGAATGGCAGCAGACATTGGAATAACCAAGATATATGCTTCTGGAAAGATGGTTTTTATGAAAACAGTTATGAATAAACATGTTTTCAACCTGATAACAGATTCAGTGGTTTCTGATGTCCACCGAAATTCTTTGGTATTTGAGGGTGACCAGATCAAGGCAGAACTTCTTCTAGAATTGCCAAGAGAACAACTCCTAAACTGGATATTTCAATGTTTGGCTGAACTACATGCTTCCTTACCTTCCCTCATAAAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarD_CdnL_TRCF DEAD Helicase_C TRCF

Protein Analysis

826

Amino Acids

93.53

Weight (kDa)

8.71

Isoelectric Point (pI)

35.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CarD_TRCF_RID PF02559 145 - 202 2.2e-09 CarD-like/TRCF RID domain
ResIII PF04851 272 - 432 3.4e-09 Type III restriction enzyme, res subunit
DEAD PF00270 274 - 436 1.7e-23 DEAD/DEAH box helicase
Helicase_C PF00271 474 - 580 1.9e-20 Helicase conserved C-terminal domain
TRCF PF03461 678 - 764 1.3e-06 TRCF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02060 AT3G02060 AT3G02060
fragaria_vesca FvH4_6g54430
malus_domestica MD17G1004500.v1.1
prunus_persica Prupe.3G314900_v2.0.a1
pyrus_communis pycom111g00110 pycom17g00110
rosa_chinensis RchiOBHm_Chr2g0176681
rosa_laevigata RLG00000022435
rosa_rugosa Rorug02G0597500
rosa_samantha Rh2BG686000 Rh2CG649300 Rh2DG700200
rosa_wichuraiana Rw0G015680 Rw2G055390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1673
Acc36I ACCTGC 1 cut(s) 1065
Acc65I GGTACC 1 cut(s) 1889
AccB1I GGYRCC 1 cut(s) 1889
AccB7I CCANNNNNTGG 2 cut(s) 35, 666
AccI GTMKAC 1 cut(s) 420
AccII CGCG 3 cut(s) 236, 291, 1303
AciI CCGC 7 cut(s) 139, 236, 332, 889, 960, 1301, 2158
AclI AACGTT 1 cut(s) 1059
AcoI YGGCCR 1 cut(s) 1450
AcsI RAATTY 6 cut(s) 282, 494, 1589, 2152, 2193, 2347
AcuI CTGAAG 1 cut(s) 945
AcyI GRCGYC 6 cut(s) 149, 155, 161, 258, 1227, 2161
AdeI CACNNNGTG 2 cut(s) 938, 1601
AfaI GTAC 4 cut(s) 350, 1891, 2060, 2477
AfiI CCNNNNNNNGG 6 cut(s) 35, 133, 463, 666, 1393, 2377
AflIII ACRYGT 1 cut(s) 2299
AjnI CCWGG 1 cut(s) 177
AjuI GAANNNNNNNTTGG 6 cut(s) 182, 214, 1115, 1147, 1206, 1238
AleI CACNNNNGTG 1 cut(s) 662
Alw26I GTCTC 4 cut(s) 324, 396, 897, 1801
Ama87I CYCGRG 1 cut(s) 2072
AoxI GGCC 8 cut(s) 136, 181, 382, 469, 582, 1192, 1450, 1696
ApeKI GCWGC 9 cut(s) 367, 370, 373, 1567, 1717, 1814, 1906, 2111, 2228
ApoI RAATTY 6 cut(s) 282, 494, 1589, 2152, 2193, 2347
ArsI GACNNNNNNTTYG 2 cut(s) 896, 928
Asp700I GAANNNNTTC 1 cut(s) 2388
Asp718I GGTACC 1 cut(s) 1889
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 7 cut(s) 136, 181, 382, 454, 520, 886, 2013
AsuHPI GGTGA 5 cut(s) 373, 455, 1613, 1913, 2378
AsuNHI GCTAGC 1 cut(s) 1340
AvaI CYCGRG 1 cut(s) 2072
AvaII GGWCC 4 cut(s) 454, 520, 886, 2013
BaeI ACNNNNGTAYC 2 cut(s) 1694, 1727
BalI TGGCCA 1 cut(s) 1452
BanI GGYRCC 1 cut(s) 1889
BbsI GAAGAC 1 cut(s) 529
BbvI GCAGC 9 cut(s) 379, 382, 385, 1579, 1704, 1826, 1918, 2098, 2240
BccI CCATC 8 cut(s) 26, 319, 548, 718, 950, 1395, 1901, 2263
BcgI CGANNNNNNTGC 2 cut(s) 569, 603
BciT130I CCWGG 1 cut(s) 179
BclI TGATCA 2 cut(s) 826, 1792
BcoDI GTCTC 4 cut(s) 324, 396, 897, 1801
BfaI CTAG 8 cut(s) 1109, 1190, 1341, 1571, 1700, 1820, 2393, 2479
BfmI CTRYAG 1 cut(s) 2112
BfuAI ACCTGC 1 cut(s) 1065
BlpI GCTNAGC 1 cut(s) 564
BmcAI AGTACT 2 cut(s) 350, 2477
Bme1390I CCNGG 1 cut(s) 179
Bme18I GGWCC 4 cut(s) 454, 520, 886, 2013
BmeT110I CYCGRG 1 cut(s) 2072
BmgT120I GGNCC 7 cut(s) 136, 181, 382, 454, 520, 886, 2013
BmiI GGNNCC 5 cut(s) 137, 383, 522, 1891, 2178
BmrFI CCNGG 1 cut(s) 179
BmrI ACTGGG 1 cut(s) 1923
BmsI GCATC 4 cut(s) 1114, 1327, 1996, 2127
BmtI GCTAGC 1 cut(s) 1344
BmuI ACTGGG 1 cut(s) 1923
BpiI GAAGAC 1 cut(s) 529
BplI GAGNNNNNCTC 6 cut(s) 862, 894, 2168, 2200, 2397, 2429
BpmI CTGGAG 3 cut(s) 371, 1347, 2149
Bpu1102I GCTNAGC 1 cut(s) 564
Bsa29I ATCGAT 1 cut(s) 266
BsaBI GATNNNNATC 2 cut(s) 1104, 1986
BsaHI GRCGYC 6 cut(s) 149, 155, 161, 258, 1227, 2161
BsaI GGTCTC 2 cut(s) 324, 396
BsaWI WCCGGW 1 cut(s) 440
BsaXI ACNNNNNCTCC 6 cut(s) 236, 266, 745, 775, 1345, 1375
Bsc4I CCNNNNNNNGG 6 cut(s) 35, 133, 463, 666, 1393, 2377
Bse1I ACTGG 5 cut(s) 660, 1330, 1918, 2015, 2426
Bse8I GATNNNNATC 2 cut(s) 1104, 1986
BseBI CCWGG 1 cut(s) 179
BseCI ATCGAT 1 cut(s) 266
BseGI GGATG 3 cut(s) 18, 1687, 1924
BseJI GATNNNNATC 2 cut(s) 1104, 1986
BseLI CCNNNNNNNGG 6 cut(s) 35, 133, 463, 666, 1393, 2377
BseMII CTCAG 4 cut(s) 815, 2046, 2094, 2139
BseNI ACTGG 5 cut(s) 660, 1330, 1918, 2015, 2426
BseRI GAGGAG 2 cut(s) 350, 2233
BseXI GCAGC 9 cut(s) 379, 382, 385, 1579, 1704, 1826, 1918, 2098, 2240
BsgI GTGCAG 1 cut(s) 115
Bsh1236I CGCG 3 cut(s) 236, 291, 1303
Bsh1285I CGRYCG 1 cut(s) 478
BshFI GGCC 8 cut(s) 138, 183, 384, 471, 584, 1194, 1452, 1698
BshNI GGYRCC 1 cut(s) 1889
BshVI ATCGAT 1 cut(s) 266
BsiEI CGRYCG 1 cut(s) 478
BsiHKCI CYCGRG 1 cut(s) 2072
BsiSI CCGG 1 cut(s) 441
BslFI GGGAC 5 cut(s) 126, 245, 1162, 1358, 1880
BslI CCNNNNNNNGG 6 cut(s) 35, 133, 463, 666, 1393, 2377
BsmAI GTCTC 4 cut(s) 324, 396, 897, 1801
BsmBI CGTCTC 1 cut(s) 897
BsmFI GGGAC 5 cut(s) 126, 245, 1162, 1358, 1880
BsmI GAATGC 2 cut(s) 1663, 1832
BsnI GGCC 8 cut(s) 138, 183, 384, 471, 584, 1194, 1452, 1698
Bso31I GGTCTC 2 cut(s) 324, 396
BsoBI CYCGRG 1 cut(s) 2072
Bsp143I GATC 5 cut(s) 718, 826, 1792, 1939, 2373
Bsp1720I GCTNAGC 1 cut(s) 564
Bsp19I CCATGG 7 cut(s) 34, 516, 783, 975, 1581, 2082, 2187
BspACI CCGC 7 cut(s) 139, 236, 332, 889, 960, 1301, 2158
BspANI GGCC 8 cut(s) 138, 183, 384, 471, 584, 1194, 1452, 1698
BspCNI CTCAG 4 cut(s) 814, 2047, 2095, 2140
BspDI ATCGAT 1 cut(s) 266
BspFNI CGCG 3 cut(s) 236, 291, 1303
BspHI TCATGA 1 cut(s) 2095
BspLI GGNNCC 5 cut(s) 137, 383, 522, 1891, 2178
BspMAI CTGCAG 1 cut(s) 2116
BspMI ACCTGC 1 cut(s) 1065
BspOI GCTAGC 1 cut(s) 1344
BspT107I GGYRCC 1 cut(s) 1889
BspTNI GGTCTC 2 cut(s) 324, 396
BsrI ACTGG 5 cut(s) 660, 1330, 1918, 2015, 2426
BssMI GATC 5 cut(s) 718, 826, 1792, 1939, 2373
BssNI GRCGYC 6 cut(s) 149, 155, 161, 258, 1227, 2161
BssT1I CCWWGG 8 cut(s) 34, 516, 684, 783, 975, 1581, 2082, 2187
Bst2UI CCWGG 1 cut(s) 179
Bst4CI ACNGT 5 cut(s) 117, 1403, 1515, 1707, 2287
Bst6I CTCTTC 2 cut(s) 438, 1949
BstACI GRCGYC 6 cut(s) 149, 155, 161, 258, 1227, 2161
BstAPI GCANNNNNTGC 1 cut(s) 938
BstC8I GCNNGC 7 cut(s) 293, 582, 593, 837, 972, 1196, 1342
BstDEI CTNAG 8 cut(s) 407, 564, 801, 1070, 1974, 2055, 2103, 2148
BstDSI CCRYGG 7 cut(s) 34, 516, 783, 975, 1581, 2082, 2187
BstEII GGTNACC 1 cut(s) 2366
BstF5I GGATG 3 cut(s) 18, 1687, 1924
BstFNI CGCG 3 cut(s) 236, 291, 1303
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 5 cut(s) 721, 829, 1795, 1942, 2376
BstMAI GTCTC 4 cut(s) 324, 396, 897, 1801
BstMBI GATC 5 cut(s) 718, 826, 1792, 1939, 2373
BstMCI CGRYCG 1 cut(s) 478
BstMWI GCNNNNNNNGC 6 cut(s) 245, 338, 364, 373, 938, 2108
BstNI CCWGG 1 cut(s) 179
BstNSI RCATGY 3 cut(s) 1623, 2303, 2453
BstPI GGTNACC 1 cut(s) 2366
BstSCI CCNGG 1 cut(s) 177
BstSFI CTRYAG 1 cut(s) 2112
BstUI CGCG 3 cut(s) 236, 291, 1303
BstV1I GCAGC 9 cut(s) 379, 382, 385, 1579, 1704, 1826, 1918, 2098, 2240
BstV2I GAAGAC 1 cut(s) 529
Bsu15I ATCGAT 1 cut(s) 266
BsuRI GGCC 8 cut(s) 138, 183, 384, 471, 584, 1194, 1452, 1698
BsuTUI ATCGAT 1 cut(s) 266
BtgI CCRYGG 7 cut(s) 34, 516, 783, 975, 1581, 2082, 2187
BtsCI GGATG 3 cut(s) 18, 1687, 1924
BtsIMutI CAGTG 2 cut(s) 667, 2331
BveI ACCTGC 1 cut(s) 1065
Cac8I GCNNGC 7 cut(s) 293, 582, 593, 837, 972, 1196, 1342
CciI TCATGA 1 cut(s) 2095
CfoI GCGC 1 cut(s) 291
Cfr13I GGNCC 7 cut(s) 136, 181, 382, 454, 520, 886, 2013
ClaI ATCGAT 1 cut(s) 266
CseI GACGC 7 cut(s) 157, 163, 169, 242, 266, 1216, 2150
Csp6I GTAC 4 cut(s) 349, 1890, 2059, 2476
CviQI GTAC 4 cut(s) 349, 1890, 2059, 2476
DdeI CTNAG 8 cut(s) 407, 564, 801, 1070, 1974, 2055, 2103, 2148
DpnI GATC 5 cut(s) 720, 828, 1794, 1941, 2375
DpnII GATC 5 cut(s) 718, 826, 1792, 1939, 2373
DraIII CACNNNGTG 2 cut(s) 938, 1601
EaeI YGGCCR 1 cut(s) 1450
Eam1104I CTCTTC 2 cut(s) 438, 1949
EarI CTCTTC 2 cut(s) 438, 1949
EciI GGCGGA 1 cut(s) 347
Eco130I CCWWGG 8 cut(s) 34, 516, 684, 783, 975, 1581, 2082, 2187
Eco147I AGGCCT 2 cut(s) 584, 1194
Eco31I GGTCTC 2 cut(s) 324, 396
Eco32I GATATC 1 cut(s) 2125
Eco47I GGWCC 4 cut(s) 454, 520, 886, 2013
Eco57I CTGAAG 1 cut(s) 945
Eco88I CYCGRG 1 cut(s) 2072
Eco91I GGTNACC 1 cut(s) 2366
EcoO109I RGGNCCY 1 cut(s) 382
EcoO65I GGTNACC 1 cut(s) 2366
EcoRI GAATTC 1 cut(s) 282
EcoRII CCWGG 1 cut(s) 177
EcoRV GATATC 1 cut(s) 2125
EcoT14I CCWWGG 8 cut(s) 34, 516, 684, 783, 975, 1581, 2082, 2187
ErhI CCWWGG 8 cut(s) 34, 516, 684, 783, 975, 1581, 2082, 2187
Esp3I CGTCTC 1 cut(s) 897
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FaqI GGGAC 5 cut(s) 126, 245, 1162, 1358, 1880
FbaI TGATCA 2 cut(s) 826, 1792
FblI GTMKAC 1 cut(s) 420
FokI GGATG 3 cut(s) 5, 1694, 1931
FspBI CTAG 8 cut(s) 1109, 1190, 1341, 1571, 1700, 1820, 2393, 2479
GlaI GCGC 1 cut(s) 290
GsuI CTGGAG 3 cut(s) 371, 1347, 2149
HaeIII GGCC 8 cut(s) 138, 183, 384, 471, 584, 1194, 1452, 1698
HapII CCGG 1 cut(s) 441
HgaI GACGC 7 cut(s) 157, 163, 169, 242, 266, 1216, 2150
HhaI GCGC 1 cut(s) 291
Hin1I GRCGYC 6 cut(s) 149, 155, 161, 258, 1227, 2161
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HincII GTYRAC 2 cut(s) 421, 1835
HindII GTYRAC 2 cut(s) 421, 1835
HindIII AAGCTT 2 cut(s) 566, 2207
HinfI GANTC 8 cut(s) 85, 251, 268, 480, 1371, 1612, 2077, 2321
HpaII CCGG 1 cut(s) 441
HphI GGTGA 5 cut(s) 373, 455, 1613, 1913, 2378
Hpy166II GTNNAC 3 cut(s) 421, 1835, 2341
Hpy8I GTNNAC 3 cut(s) 421, 1835, 2341
Hpy99I CGWCG 5 cut(s) 157, 163, 169, 231, 313
HpyAV CCTTC 5 cut(s) 457, 1719, 2061, 2113, 2472
HpyCH4III ACNGT 5 cut(s) 117, 1403, 1515, 1707, 2287
HpyCH4IV ACGT 3 cut(s) 534, 906, 1059
HpyF10VI GCNNNNNNNGC 6 cut(s) 245, 338, 364, 373, 938, 2108
HpyF3I CTNAG 8 cut(s) 407, 564, 801, 1070, 1974, 2055, 2103, 2148
HpySE526I ACGT 3 cut(s) 534, 906, 1059
Hsp92I GRCGYC 6 cut(s) 149, 155, 161, 258, 1227, 2161
HspAI GCGC 1 cut(s) 289
KpnI GGTACC 1 cut(s) 1893
Ksp22I TGATCA 2 cut(s) 826, 1792
Kzo9I GATC 5 cut(s) 718, 826, 1792, 1939, 2373
LmnI GCTCC 4 cut(s) 15, 355, 511, 2176
Lsp1109I GCAGC 9 cut(s) 379, 382, 385, 1579, 1704, 1826, 1918, 2098, 2240
LweI GCATC 4 cut(s) 1114, 1327, 1996, 2127
MaeI CTAG 8 cut(s) 1109, 1190, 1341, 1571, 1700, 1820, 2393, 2479
MaeII ACGT 3 cut(s) 534, 906, 1059
MaeIII GTNAC 4 cut(s) 410, 602, 1779, 2366
MalI GATC 5 cut(s) 720, 828, 1794, 1941, 2375
MboI GATC 5 cut(s) 718, 826, 1792, 1939, 2373
MfeI CAATTG 1 cut(s) 993
MlsI TGGCCA 1 cut(s) 1452
MluNI TGGCCA 1 cut(s) 1452
MlyI GAGTC 3 cut(s) 94, 260, 1380
MmeI TCCRAC 6 cut(s) 234, 291, 1042, 1782, 1876, 1903
Mox20I TGGCCA 1 cut(s) 1452
MroXI GAANNNNTTC 1 cut(s) 2388
MscI TGGCCA 1 cut(s) 1452
MseI TTAA 6 cut(s) 492, 576, 863, 893, 1428, 2201
MslI CAYNNNNRTG 1 cut(s) 662
Msp20I TGGCCA 1 cut(s) 1452
MspA1I CMGCKG 1 cut(s) 1717
MspI CCGG 1 cut(s) 441
MspR9I CCNGG 1 cut(s) 179
MunI CAATTG 1 cut(s) 993
Mva1269I GAATGC 2 cut(s) 1663, 1832
MvaI CCWGG 1 cut(s) 179
MvnI CGCG 3 cut(s) 236, 291, 1303
MwoI GCNNNNNNNGC 6 cut(s) 245, 338, 364, 373, 938, 2108
NcoI CCATGG 7 cut(s) 34, 516, 783, 975, 1581, 2082, 2187
NdeII GATC 5 cut(s) 718, 826, 1792, 1939, 2373
NheI GCTAGC 1 cut(s) 1340
NlaIV GGNNCC 5 cut(s) 137, 383, 522, 1891, 2178
NmeAIII GCCGAG 1 cut(s) 1373
NmuCI GTSAC 2 cut(s) 1779, 2366
NspI RCATGY 3 cut(s) 1623, 2303, 2453
OliI CACNNNNGTG 1 cut(s) 662
PaeR7I CTCGAG 1 cut(s) 2072
PagI TCATGA 1 cut(s) 2095
PceI AGGCCT 2 cut(s) 584, 1194
PciI ACATGT 1 cut(s) 2299
PcsI WCGNNNNNNNCGW 4 cut(s) 80, 155, 161, 480
PctI GAATGC 2 cut(s) 1663, 1832
PdmI GAANNNNTTC 1 cut(s) 2388
PfeI GAWTC 5 cut(s) 268, 480, 1612, 2077, 2321
PflMI CCANNNNNTGG 2 cut(s) 35, 666
PleI GAGTC 3 cut(s) 93, 259, 1379
PpsI GAGTC 3 cut(s) 93, 259, 1379
PscI ACATGT 1 cut(s) 2299
PsiI TTATAA 1 cut(s) 1673
Psp1406I AACGTT 1 cut(s) 1059
Psp6I CCWGG 1 cut(s) 177
PspEI GGTNACC 1 cut(s) 2366
PspGI CCWGG 1 cut(s) 177
PspN4I GGNNCC 5 cut(s) 137, 383, 522, 1891, 2178
PspPI GGNCC 7 cut(s) 136, 181, 382, 454, 520, 886, 2013
PsrI GAACNNNNNNTAC 2 cut(s) 1986, 2018
PstI CTGCAG 1 cut(s) 2116
PvuII CAGCTG 1 cut(s) 1717
RsaI GTAC 4 cut(s) 350, 1891, 2060, 2477
RsaNI GTAC 4 cut(s) 349, 1890, 2059, 2476
RseI CAYNNNNRTG 1 cut(s) 662
SalI GTCGAC 1 cut(s) 419
SaqAI TTAA 6 cut(s) 492, 576, 863, 893, 1428, 2201
Sau3AI GATC 5 cut(s) 718, 826, 1792, 1939, 2373
Sau96I GGNCC 7 cut(s) 136, 181, 382, 454, 520, 886, 2013
ScaI AGTACT 2 cut(s) 350, 2477
SchI GAGTC 3 cut(s) 94, 260, 1380
ScrFI CCNGG 1 cut(s) 179
SfaNI GCATC 4 cut(s) 1114, 1327, 1996, 2127
SfcI CTRYAG 1 cut(s) 2112
Sfr274I CTCGAG 1 cut(s) 2072
SinI GGWCC 4 cut(s) 454, 520, 886, 2013
SlaI CTCGAG 1 cut(s) 2072
SmiMI CAYNNNNRTG 1 cut(s) 662
SmlI CTYRAG 1 cut(s) 2072
SmoI CTYRAG 1 cut(s) 2072
SseBI AGGCCT 2 cut(s) 584, 1194
SsiI CCGC 7 cut(s) 139, 236, 332, 889, 960, 1301, 2158
SspI AATATT 1 cut(s) 1559
SspMI CTAG 8 cut(s) 1109, 1190, 1341, 1571, 1700, 1820, 2393, 2479
StuI AGGCCT 2 cut(s) 584, 1194
StyD4I CCNGG 1 cut(s) 177
StyI CCWWGG 8 cut(s) 34, 516, 684, 783, 975, 1581, 2082, 2187
TaaI ACNGT 5 cut(s) 117, 1403, 1515, 1707, 2287
TaiI ACGT 3 cut(s) 537, 909, 1062
TaqI TCGA 8 cut(s) 74, 215, 266, 420, 478, 498, 1990, 2073
TaqII GACCGA 1 cut(s) 464
TatI WGTACW 3 cut(s) 348, 2058, 2475
TauI GCSGC 3 cut(s) 141, 239, 2161
TfiI GAWTC 5 cut(s) 268, 480, 1612, 2077, 2321
Tru1I TTAA 6 cut(s) 492, 576, 863, 893, 1428, 2201
Tru9I TTAA 6 cut(s) 492, 576, 863, 893, 1428, 2201
TscAI CASTG 2 cut(s) 667, 2331
TseFI GTSAC 2 cut(s) 1779, 2366
TseI GCWGC 9 cut(s) 367, 370, 373, 1567, 1717, 1814, 1906, 2111, 2228
Tsp45I GTSAC 2 cut(s) 1779, 2366
TspDTI ATGAA 9 cut(s) 529, 832, 1224, 1242, 2045, 2084, 2112, 2294, 2306
TspGWI ACGGA 3 cut(s) 798, 926, 2005
TspRI CASTG 2 cut(s) 667, 2331
Van91I CCANNNNNTGG 2 cut(s) 35, 666
VpaK11BI GGWCC 4 cut(s) 454, 520, 886, 2013
XapI RAATTY 6 cut(s) 282, 494, 1589, 2152, 2193, 2347
XbaI TCTAGA 3 cut(s) 1108, 1819, 2392
XceI RCATGY 3 cut(s) 1623, 2303, 2453
XcmI CCANNNNNNNNNTGG 1 cut(s) 706
XhoI CTCGAG 1 cut(s) 2072
XmiI GTMKAC 1 cut(s) 420
XmnI GAANNNNTTC 1 cut(s) 2388
XspI CTAG 8 cut(s) 1109, 1190, 1341, 1571, 1700, 1820, 2393, 2479
ZrmI AGTACT 2 cut(s) 350, 2477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.