Rw0G015680

CarD-like/TRCF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00723
OG0015231
Physical Location & Seq
Forward (+)
24062 .. 30500
6439 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G015680.1

Sequence Viewer

Length: 2466 bp
ATGGCTCTGCTCCTCCCCATCCCAATCCCAATCCATGGCGTTTCTTCTTCTTCTTGTTATAGGTCGAGGTCGGCGAGTGGGCTGTGCAGGGTTTGGGGTTGGGGACTGTTGAAAATCCAGAGAGGGGGCCGCACCCAGACGCCGACGCCGACGAAGAACAGCCTGGGCCTTTCCAATTCCACTTTCACGAAAACCAAATCGAGCAAAAAGCGACGGGAGGCGGCTGTGGAGGCAGAGTCGGACGCCATATCGATTCTAAACGAGAGAATTCGGCGCGAGCAGAGAAAGAAATCGTCGGAGGGGTCTCCGATGGAGGCGGAGGAGGCAGACAAGTACTTGGAGCTGGTGAAGCAGCAGCAGCAGAGGGGCCTCCAGAAGCTCAAAGGAGACCTTAGTTACAAGGTCGACCCCTACACTCTCCGTTCCGGTGATTATGTGGTCCATAAGAAGGTCGGCCTCGGTCGATTCGTTGGGATTAAATTCGACAATGCTCCTGCTTCCATGGACCCTATTGAATACGTCTTCATTGAGTATGCTGATGGCATGGCTAAGCTTCCTGTTAAGCAGGCCTCTCGCTTGCTCTATCGTTACAATCTCCCCAACGAAAACAAAAAACCTCACACATTGAGCAAACTGAACGACACCAGTGTGTGGGAGAGAAGAAAGACCAAGGGAAAGATTGCCATTCAGAAAATGGTTGTTGATCTGATGGAGTTGTATTTACACAGGCTCAAACAAACACGCCCTCCCTACCCTCTTACTCCTGCCATGGCTCACTTTGTTTCTCAGTTTCCGTATGAGCCTACTCCTGATCAAAAGCAGGCTTTCATTGATGTTGAGAAAGATTTAACAGGGAGGGAAACTCCAATGGACCGCTTAATTTGTGGAGACGTTGGCTTTGGCAAAACTGAAGTTGCACTCCGTGCCATCTTTTGTGTAGTTTCCGCAGGGAAGCAAGCCATGGTTCTTGCACCCACAATTGTTTTAGCCAAGCAACATTTTGAAGTTATCTCACAGCGATTTTCTATCTACCCAAATATCAACGTTGGACTCCTTAGCAGGTTTCAGACTAAATCAGAGAAAGATGAGCATCTAGACATGATAAAGAATGGTCATTTGGACATAATTGTTGGGACACACTCACTTCTTGGAAGTCGTGTTGTGTATAATAATCTAGGCCTGCTTGTCGTTGATGAAGAACAGAGGTTTGGCGTCAAACAGAAAGAGAAGATTGCTTCATTCAAAACATCAGTTGATGTTCTTACTCTATCTGCAACACCTATACCGCGAACCCTTTATTTAGCATTGACTGGTTTTCGTGATGCTAGCTTGATTTCAACTCCACCTCCAGAAAGAGTCCCTATAAGAACCCATCTCTCGGCATACAGTAAAGAAAAGGTATTATCAGCAATTAAGTATGAGCTGGAACGAGGTGGCCAAGTATTTTATGTTTTGCCTCGTATCAAAGGACTTGAAGAAGTGATGAGTTTTCTTGAACAGTCGTTTCCTGATGTTGAAATAGCTATTGCACACGGGAAGCAATATTCAAAGCAGCTAGAGGAAACCATGGAGAAATTTTCACAAGGTGAAATCAAGATTCTTACATGCACTAATATAGTAGAAAGTGGGCTTGACATTCAGAATGCAAACACCATTATAATTCAGGATGTTCAACAATTTGGCCTAGCACAGTTGTATCAGCTGCGGGGAAGGGTGGGGAGGGCAGATAAGGAAGCTCACGCACACTTATTTTATCCAGAGAAGTCACTTCTAACTGATCAGGCATTGGAGAGACTTGCAGCTCTAGAGGAATGCTGTCAACTTGGGCAAGGTTTCCAACTTGCAGAGAGGGACATGGGAATAAGAGGCTTTGGTACCATCTTTGGTGAGCAGCAGACTGGGGATGTTGGAAATGTTGGCATTGATCTCTTCTTTGAAATGCTGTTTGAGAGCTTGTCTAAGGTTGATGAGCATCGAGTAGTTTCAGTTCCTTACTGGTCCGTTGAGATTGATATGAATATAAATCCTCATCTCCCTTCTGAGTACATAAATAATCTCGAGAATCCCATGGAGATAATTCATGAAGCTGAGAAAGCTGCAGAGAAGGATATCTGGAGTTTGATGCAATATACTGAGAATTTGCGGCGTCAATATGGAAAGGAGCCACACTCCATGGAAATTTTATTAAAGAAGCTTTATGTGAGGAGAATGGCAGCAGACATTGGAATAACCAAGATATATGCTTCTGGAAAGATGGTTTTTATGAAAACAGTTATGAATAAACATGTTTTCAATCTGATAACAGATTCAGTGGTTTCTGATGTCCACCGAAATTCTTTGGTATTTGAGGGTGACCAGATCAAGGCAGAACTTCTTCTAGAATTGCCAAGAGAACAACTCCTAAACTGGATATTTCGATGTTTGGCTGAACTACATGCTTCCTTACCTTCCCTCATAAAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarD_CdnL_TRCF DEAD Helicase_C TRCF

Protein Analysis

821

Amino Acids

93.01

Weight (kDa)

8.71

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CarD_TRCF_RID PF02559 140 - 197 2.1e-09 CarD-like/TRCF RID domain
ResIII PF04851 267 - 427 3.3e-09 Type III restriction enzyme, res subunit
DEAD PF00270 269 - 431 1.7e-23 DEAD/DEAH box helicase
Helicase_C PF00271 469 - 575 1.9e-20 Helicase conserved C-terminal domain
TRCF PF03461 673 - 759 1.3e-06 TRCF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02060 AT3G02060 AT3G02060
fragaria_vesca FvH4_6g54430
malus_domestica MD17G1004500.v1.1
prunus_persica Prupe.3G314900_v2.0.a1
pyrus_communis pycom111g00110 pycom17g00110
rosa_chinensis RchiOBHm_Chr2g0176681
rosa_laevigata RLG00000022435
rosa_rugosa Rorug02G0597500
rosa_samantha Rh2BG686000 Rh2CG649300 Rh2DG700200
rosa_wichuraiana Rw0G015680 Rw2G055390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1658
Acc36I ACCTGC 1 cut(s) 1050
Acc65I GGTACC 1 cut(s) 1874
AccB1I GGYRCC 1 cut(s) 1874
AccB7I CCANNNNNTGG 2 cut(s) 35, 651
AccI GTMKAC 1 cut(s) 405
AccII CGCG 2 cut(s) 276, 1288
AciI CCGC 8 cut(s) 130, 221, 317, 874, 945, 1286, 1705, 2143
AclI AACGTT 1 cut(s) 1044
AcoI YGGCCR 1 cut(s) 1435
AcsI RAATTY 6 cut(s) 267, 479, 1574, 2137, 2178, 2332
AcuI CTGAAG 1 cut(s) 930
AcyI GRCGYC 5 cut(s) 140, 146, 243, 1212, 2146
AdeI CACNNNGTG 2 cut(s) 923, 1586
AfaI GTAC 4 cut(s) 335, 1876, 2045, 2462
AfiI CCNNNNNNNGG 6 cut(s) 35, 124, 448, 651, 1378, 2362
AflIII ACRYGT 1 cut(s) 2284
AjnI CCWGG 1 cut(s) 162
AjuI GAANNNNNNNTTGG 6 cut(s) 167, 199, 1100, 1132, 1191, 1223
AleI CACNNNNGTG 1 cut(s) 647
Alw26I GTCTC 4 cut(s) 309, 381, 882, 1786
Ama87I CYCGRG 1 cut(s) 2057
AoxI GGCC 8 cut(s) 127, 166, 367, 454, 567, 1177, 1435, 1681
ApeKI GCWGC 9 cut(s) 352, 355, 358, 1552, 1702, 1799, 1891, 2096, 2213
ApoI RAATTY 6 cut(s) 267, 479, 1574, 2137, 2178, 2332
ArsI GACNNNNNNTTYG 2 cut(s) 881, 913
Asp700I GAANNNNTTC 1 cut(s) 2373
Asp718I GGTACC 1 cut(s) 1874
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 7 cut(s) 127, 166, 367, 439, 505, 871, 1998
AsuHPI GGTGA 5 cut(s) 358, 440, 1598, 1898, 2363
AsuNHI GCTAGC 1 cut(s) 1325
AvaI CYCGRG 1 cut(s) 2057
AvaII GGWCC 4 cut(s) 439, 505, 871, 1998
BaeI ACNNNNGTAYC 2 cut(s) 1679, 1712
BalI TGGCCA 1 cut(s) 1437
BanI GGYRCC 1 cut(s) 1874
BbsI GAAGAC 1 cut(s) 514
BbvI GCAGC 9 cut(s) 364, 367, 370, 1564, 1689, 1811, 1903, 2083, 2225
BccI CCATC 8 cut(s) 26, 304, 533, 703, 935, 1380, 1886, 2248
BcgI CGANNNNNNTGC 2 cut(s) 554, 588
BciT130I CCWGG 1 cut(s) 164
BclI TGATCA 2 cut(s) 811, 1777
BcoDI GTCTC 4 cut(s) 309, 381, 882, 1786
BfaI CTAG 8 cut(s) 1094, 1175, 1326, 1556, 1685, 1805, 2378, 2464
BfmI CTRYAG 1 cut(s) 2097
BfuAI ACCTGC 1 cut(s) 1050
BlpI GCTNAGC 1 cut(s) 549
BmcAI AGTACT 2 cut(s) 335, 2462
Bme1390I CCNGG 1 cut(s) 164
Bme18I GGWCC 4 cut(s) 439, 505, 871, 1998
BmeT110I CYCGRG 1 cut(s) 2057
BmgT120I GGNCC 7 cut(s) 127, 166, 367, 439, 505, 871, 1998
BmiI GGNNCC 5 cut(s) 128, 368, 507, 1876, 2163
BmrFI CCNGG 1 cut(s) 164
BmrI ACTGGG 1 cut(s) 1908
BmsI GCATC 4 cut(s) 1099, 1312, 1981, 2112
BmtI GCTAGC 1 cut(s) 1329
BmuI ACTGGG 1 cut(s) 1908
BpiI GAAGAC 1 cut(s) 514
BplI GAGNNNNNCTC 6 cut(s) 847, 879, 2153, 2185, 2382, 2414
BpmI CTGGAG 3 cut(s) 356, 1332, 2134
Bpu10I CCTNAGC 1 cut(s) 1055
Bpu1102I GCTNAGC 1 cut(s) 549
Bsa29I ATCGAT 1 cut(s) 251
BsaBI GATNNNNATC 2 cut(s) 1089, 1971
BsaHI GRCGYC 5 cut(s) 140, 146, 243, 1212, 2146
BsaI GGTCTC 2 cut(s) 309, 381
BsaWI WCCGGW 1 cut(s) 425
BsaXI ACNNNNNCTCC 6 cut(s) 221, 251, 730, 760, 1330, 1360
Bsc4I CCNNNNNNNGG 6 cut(s) 35, 124, 448, 651, 1378, 2362
Bse1I ACTGG 5 cut(s) 645, 1315, 1903, 2000, 2411
Bse8I GATNNNNATC 2 cut(s) 1089, 1971
BseBI CCWGG 1 cut(s) 164
BseCI ATCGAT 1 cut(s) 251
BseGI GGATG 3 cut(s) 18, 1672, 1909
BseJI GATNNNNATC 2 cut(s) 1089, 1971
BseLI CCNNNNNNNGG 6 cut(s) 35, 124, 448, 651, 1378, 2362
BseMII CTCAG 4 cut(s) 800, 2031, 2079, 2124
BseNI ACTGG 5 cut(s) 645, 1315, 1903, 2000, 2411
BseRI GAGGAG 2 cut(s) 335, 2218
BseXI GCAGC 9 cut(s) 364, 367, 370, 1564, 1689, 1811, 1903, 2083, 2225
BsgI GTGCAG 1 cut(s) 106
Bsh1236I CGCG 2 cut(s) 276, 1288
Bsh1285I CGRYCG 1 cut(s) 463
BshFI GGCC 8 cut(s) 129, 168, 369, 456, 569, 1179, 1437, 1683
BshNI GGYRCC 1 cut(s) 1874
BshVI ATCGAT 1 cut(s) 251
BsiEI CGRYCG 1 cut(s) 463
BsiHKCI CYCGRG 1 cut(s) 2057
BsiSI CCGG 1 cut(s) 426
BslFI GGGAC 4 cut(s) 117, 1147, 1343, 1865
BslI CCNNNNNNNGG 6 cut(s) 35, 124, 448, 651, 1378, 2362
BsmAI GTCTC 4 cut(s) 309, 381, 882, 1786
BsmBI CGTCTC 1 cut(s) 882
BsmFI GGGAC 4 cut(s) 117, 1147, 1343, 1865
BsmI GAATGC 2 cut(s) 1648, 1817
BsnI GGCC 8 cut(s) 129, 168, 369, 456, 569, 1179, 1437, 1683
Bso31I GGTCTC 2 cut(s) 309, 381
BsoBI CYCGRG 1 cut(s) 2057
Bsp143I GATC 5 cut(s) 703, 811, 1777, 1924, 2358
Bsp1720I GCTNAGC 1 cut(s) 549
Bsp19I CCATGG 7 cut(s) 34, 501, 768, 960, 1566, 2067, 2172
BspACI CCGC 8 cut(s) 130, 221, 317, 874, 945, 1286, 1705, 2143
BspANI GGCC 8 cut(s) 129, 168, 369, 456, 569, 1179, 1437, 1683
BspCNI CTCAG 4 cut(s) 799, 2032, 2080, 2125
BspDI ATCGAT 1 cut(s) 251
BspFNI CGCG 2 cut(s) 276, 1288
BspHI TCATGA 1 cut(s) 2080
BspLI GGNNCC 5 cut(s) 128, 368, 507, 1876, 2163
BspMAI CTGCAG 1 cut(s) 2101
BspMI ACCTGC 1 cut(s) 1050
BspOI GCTAGC 1 cut(s) 1329
BspT107I GGYRCC 1 cut(s) 1874
BspTNI GGTCTC 2 cut(s) 309, 381
BsrI ACTGG 5 cut(s) 645, 1315, 1903, 2000, 2411
BssMI GATC 5 cut(s) 703, 811, 1777, 1924, 2358
BssNI GRCGYC 5 cut(s) 140, 146, 243, 1212, 2146
BssT1I CCWWGG 8 cut(s) 34, 501, 669, 768, 960, 1566, 2067, 2172
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 5 cut(s) 108, 1388, 1500, 1692, 2272
Bst6I CTCTTC 1 cut(s) 1934
BstACI GRCGYC 5 cut(s) 140, 146, 243, 1212, 2146
BstAPI GCANNNNNTGC 1 cut(s) 923
BstC8I GCNNGC 7 cut(s) 278, 567, 578, 822, 957, 1181, 1327
BstDEI CTNAG 8 cut(s) 392, 549, 786, 1055, 1959, 2040, 2088, 2133
BstDSI CCRYGG 7 cut(s) 34, 501, 768, 960, 1566, 2067, 2172
BstEII GGTNACC 1 cut(s) 2351
BstF5I GGATG 3 cut(s) 18, 1672, 1909
BstFNI CGCG 2 cut(s) 276, 1288
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 5 cut(s) 706, 814, 1780, 1927, 2361
BstMAI GTCTC 4 cut(s) 309, 381, 882, 1786
BstMBI GATC 5 cut(s) 703, 811, 1777, 1924, 2358
BstMCI CGRYCG 1 cut(s) 463
BstMWI GCNNNNNNNGC 6 cut(s) 230, 323, 349, 358, 923, 2093
BstNI CCWGG 1 cut(s) 164
BstNSI RCATGY 3 cut(s) 1608, 2288, 2438
BstPI GGTNACC 1 cut(s) 2351
BstSCI CCNGG 1 cut(s) 162
BstSFI CTRYAG 1 cut(s) 2097
BstUI CGCG 2 cut(s) 276, 1288
BstV1I GCAGC 9 cut(s) 364, 367, 370, 1564, 1689, 1811, 1903, 2083, 2225
BstV2I GAAGAC 1 cut(s) 514
Bsu15I ATCGAT 1 cut(s) 251
BsuRI GGCC 8 cut(s) 129, 168, 369, 456, 569, 1179, 1437, 1683
BsuTUI ATCGAT 1 cut(s) 251
BtgI CCRYGG 7 cut(s) 34, 501, 768, 960, 1566, 2067, 2172
BtsCI GGATG 3 cut(s) 18, 1672, 1909
BtsIMutI CAGTG 2 cut(s) 652, 2316
BveI ACCTGC 1 cut(s) 1050
Cac8I GCNNGC 7 cut(s) 278, 567, 578, 822, 957, 1181, 1327
CciI TCATGA 1 cut(s) 2080
CfoI GCGC 1 cut(s) 276
Cfr13I GGNCC 7 cut(s) 127, 166, 367, 439, 505, 871, 1998
ClaI ATCGAT 1 cut(s) 251
CseI GACGC 5 cut(s) 148, 154, 251, 1201, 2135
Csp6I GTAC 4 cut(s) 334, 1875, 2044, 2461
CviQI GTAC 4 cut(s) 334, 1875, 2044, 2461
DdeI CTNAG 8 cut(s) 392, 549, 786, 1055, 1959, 2040, 2088, 2133
DpnI GATC 5 cut(s) 705, 813, 1779, 1926, 2360
DpnII GATC 5 cut(s) 703, 811, 1777, 1924, 2358
DraIII CACNNNGTG 2 cut(s) 923, 1586
EaeI YGGCCR 1 cut(s) 1435
Eam1104I CTCTTC 1 cut(s) 1934
EarI CTCTTC 1 cut(s) 1934
EciI GGCGGA 1 cut(s) 332
Eco130I CCWWGG 8 cut(s) 34, 501, 669, 768, 960, 1566, 2067, 2172
Eco147I AGGCCT 2 cut(s) 569, 1179
Eco31I GGTCTC 2 cut(s) 309, 381
Eco32I GATATC 1 cut(s) 2110
Eco47I GGWCC 4 cut(s) 439, 505, 871, 1998
Eco57I CTGAAG 1 cut(s) 930
Eco88I CYCGRG 1 cut(s) 2057
Eco91I GGTNACC 1 cut(s) 2351
EcoO109I RGGNCCY 1 cut(s) 367
EcoO65I GGTNACC 1 cut(s) 2351
EcoRI GAATTC 1 cut(s) 267
EcoRII CCWGG 1 cut(s) 162
EcoRV GATATC 1 cut(s) 2110
EcoT14I CCWWGG 8 cut(s) 34, 501, 669, 768, 960, 1566, 2067, 2172
ErhI CCWWGG 8 cut(s) 34, 501, 669, 768, 960, 1566, 2067, 2172
Esp3I CGTCTC 1 cut(s) 882
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FaqI GGGAC 4 cut(s) 117, 1147, 1343, 1865
FauI CCCGC 1 cut(s) 1698
FbaI TGATCA 2 cut(s) 811, 1777
FblI GTMKAC 1 cut(s) 405
FokI GGATG 3 cut(s) 5, 1679, 1916
FspBI CTAG 8 cut(s) 1094, 1175, 1326, 1556, 1685, 1805, 2378, 2464
GlaI GCGC 1 cut(s) 275
GsuI CTGGAG 3 cut(s) 356, 1332, 2134
HaeIII GGCC 8 cut(s) 129, 168, 369, 456, 569, 1179, 1437, 1683
HapII CCGG 1 cut(s) 426
HgaI GACGC 5 cut(s) 148, 154, 251, 1201, 2135
HhaI GCGC 1 cut(s) 276
Hin1I GRCGYC 5 cut(s) 140, 146, 243, 1212, 2146
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HincII GTYRAC 2 cut(s) 406, 1820
HindII GTYRAC 2 cut(s) 406, 1820
HindIII AAGCTT 2 cut(s) 551, 2192
HinfI GANTC 8 cut(s) 236, 253, 465, 1050, 1356, 1597, 2062, 2306
HpaII CCGG 1 cut(s) 426
HphI GGTGA 5 cut(s) 358, 440, 1598, 1898, 2363
Hpy166II GTNNAC 3 cut(s) 406, 1820, 2326
Hpy8I GTNNAC 3 cut(s) 406, 1820, 2326
Hpy99I CGWCG 4 cut(s) 148, 154, 216, 298
HpyAV CCTTC 5 cut(s) 442, 1704, 2046, 2098, 2457
HpyCH4III ACNGT 5 cut(s) 108, 1388, 1500, 1692, 2272
HpyCH4IV ACGT 3 cut(s) 519, 891, 1044
HpyF10VI GCNNNNNNNGC 6 cut(s) 230, 323, 349, 358, 923, 2093
HpyF3I CTNAG 8 cut(s) 392, 549, 786, 1055, 1959, 2040, 2088, 2133
HpySE526I ACGT 3 cut(s) 519, 891, 1044
Hsp92I GRCGYC 5 cut(s) 140, 146, 243, 1212, 2146
HspAI GCGC 1 cut(s) 274
KpnI GGTACC 1 cut(s) 1878
Ksp22I TGATCA 2 cut(s) 811, 1777
Kzo9I GATC 5 cut(s) 703, 811, 1777, 1924, 2358
LmnI GCTCC 4 cut(s) 15, 340, 496, 2161
Lsp1109I GCAGC 9 cut(s) 364, 367, 370, 1564, 1689, 1811, 1903, 2083, 2225
LweI GCATC 4 cut(s) 1099, 1312, 1981, 2112
MaeI CTAG 8 cut(s) 1094, 1175, 1326, 1556, 1685, 1805, 2378, 2464
MaeII ACGT 3 cut(s) 519, 891, 1044
MaeIII GTNAC 4 cut(s) 395, 587, 1764, 2351
MalI GATC 5 cut(s) 705, 813, 1779, 1926, 2360
MboI GATC 5 cut(s) 703, 811, 1777, 1924, 2358
MfeI CAATTG 1 cut(s) 978
MlsI TGGCCA 1 cut(s) 1437
MluNI TGGCCA 1 cut(s) 1437
MlyI GAGTC 3 cut(s) 245, 1044, 1365
MmeI TCCRAC 5 cut(s) 219, 276, 1027, 1861, 1888
Mox20I TGGCCA 1 cut(s) 1437
MroXI GAANNNNTTC 1 cut(s) 2373
MscI TGGCCA 1 cut(s) 1437
MseI TTAA 6 cut(s) 477, 561, 848, 878, 1413, 2186
MslI CAYNNNNRTG 1 cut(s) 647
Msp20I TGGCCA 1 cut(s) 1437
MspA1I CMGCKG 1 cut(s) 1702
MspI CCGG 1 cut(s) 426
MspR9I CCNGG 1 cut(s) 164
MunI CAATTG 1 cut(s) 978
Mva1269I GAATGC 2 cut(s) 1648, 1817
MvaI CCWGG 1 cut(s) 164
MvnI CGCG 2 cut(s) 276, 1288
MwoI GCNNNNNNNGC 6 cut(s) 230, 323, 349, 358, 923, 2093
NcoI CCATGG 7 cut(s) 34, 501, 768, 960, 1566, 2067, 2172
NdeII GATC 5 cut(s) 703, 811, 1777, 1924, 2358
NheI GCTAGC 1 cut(s) 1325
NlaIV GGNNCC 5 cut(s) 128, 368, 507, 1876, 2163
NmeAIII GCCGAG 1 cut(s) 1358
NmuCI GTSAC 2 cut(s) 1764, 2351
NspI RCATGY 3 cut(s) 1608, 2288, 2438
OliI CACNNNNGTG 1 cut(s) 647
PaeR7I CTCGAG 1 cut(s) 2057
PagI TCATGA 1 cut(s) 2080
PceI AGGCCT 2 cut(s) 569, 1179
PciI ACATGT 1 cut(s) 2284
PcsI WCGNNNNNNNCGW 3 cut(s) 71, 146, 465
PctI GAATGC 2 cut(s) 1648, 1817
PdmI GAANNNNTTC 1 cut(s) 2373
PfeI GAWTC 5 cut(s) 253, 465, 1597, 2062, 2306
PflMI CCANNNNNTGG 2 cut(s) 35, 651
PleI GAGTC 3 cut(s) 244, 1044, 1364
PpsI GAGTC 3 cut(s) 244, 1044, 1364
PscI ACATGT 1 cut(s) 2284
PsiI TTATAA 1 cut(s) 1658
Psp1406I AACGTT 1 cut(s) 1044
Psp6I CCWGG 1 cut(s) 162
PspEI GGTNACC 1 cut(s) 2351
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 5 cut(s) 128, 368, 507, 1876, 2163
PspPI GGNCC 7 cut(s) 127, 166, 367, 439, 505, 871, 1998
PsrI GAACNNNNNNTAC 2 cut(s) 1971, 2003
PstI CTGCAG 1 cut(s) 2101
PvuII CAGCTG 1 cut(s) 1702
RsaI GTAC 4 cut(s) 335, 1876, 2045, 2462
RsaNI GTAC 4 cut(s) 334, 1875, 2044, 2461
RseI CAYNNNNRTG 1 cut(s) 647
SalI GTCGAC 1 cut(s) 404
SaqAI TTAA 6 cut(s) 477, 561, 848, 878, 1413, 2186
Sau3AI GATC 5 cut(s) 703, 811, 1777, 1924, 2358
Sau96I GGNCC 7 cut(s) 127, 166, 367, 439, 505, 871, 1998
ScaI AGTACT 2 cut(s) 335, 2462
SchI GAGTC 3 cut(s) 245, 1044, 1365
ScrFI CCNGG 1 cut(s) 164
SfaNI GCATC 4 cut(s) 1099, 1312, 1981, 2112
SfcI CTRYAG 1 cut(s) 2097
Sfr274I CTCGAG 1 cut(s) 2057
SinI GGWCC 4 cut(s) 439, 505, 871, 1998
SlaI CTCGAG 1 cut(s) 2057
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 1 cut(s) 2057
SmoI CTYRAG 1 cut(s) 2057
SseBI AGGCCT 2 cut(s) 569, 1179
SsiI CCGC 8 cut(s) 130, 221, 317, 874, 945, 1286, 1705, 2143
SspI AATATT 1 cut(s) 1544
SspMI CTAG 8 cut(s) 1094, 1175, 1326, 1556, 1685, 1805, 2378, 2464
StuI AGGCCT 2 cut(s) 569, 1179
StyD4I CCNGG 1 cut(s) 162
StyI CCWWGG 8 cut(s) 34, 501, 669, 768, 960, 1566, 2067, 2172
TaaI ACNGT 5 cut(s) 108, 1388, 1500, 1692, 2272
TaiI ACGT 3 cut(s) 522, 894, 1047
TaqI TCGA 9 cut(s) 65, 200, 251, 405, 463, 483, 1975, 2058, 2416
TaqII GACCGA 1 cut(s) 449
TatI WGTACW 3 cut(s) 333, 2043, 2460
TauI GCSGC 3 cut(s) 132, 224, 2146
TfiI GAWTC 5 cut(s) 253, 465, 1597, 2062, 2306
Tru1I TTAA 6 cut(s) 477, 561, 848, 878, 1413, 2186
Tru9I TTAA 6 cut(s) 477, 561, 848, 878, 1413, 2186
TscAI CASTG 2 cut(s) 652, 2316
TseFI GTSAC 2 cut(s) 1764, 2351
TseI GCWGC 9 cut(s) 352, 355, 358, 1552, 1702, 1799, 1891, 2096, 2213
Tsp45I GTSAC 2 cut(s) 1764, 2351
TspDTI ATGAA 9 cut(s) 514, 817, 1209, 1227, 2030, 2069, 2097, 2279, 2291
TspGWI ACGGA 4 cut(s) 410, 783, 911, 1990
TspRI CASTG 2 cut(s) 652, 2316
Van91I CCANNNNNTGG 2 cut(s) 35, 651
VpaK11BI GGWCC 4 cut(s) 439, 505, 871, 1998
XapI RAATTY 6 cut(s) 267, 479, 1574, 2137, 2178, 2332
XbaI TCTAGA 3 cut(s) 1093, 1804, 2377
XceI RCATGY 3 cut(s) 1608, 2288, 2438
XcmI CCANNNNNNNNNTGG 1 cut(s) 691
XhoI CTCGAG 1 cut(s) 2057
XmiI GTMKAC 1 cut(s) 405
XmnI GAANNNNTTC 1 cut(s) 2373
XspI CTAG 8 cut(s) 1094, 1175, 1326, 1556, 1685, 1805, 2378, 2464
ZrmI AGTACT 2 cut(s) 335, 2462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.