FvH4_6g54430

CarD-like/TRCF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015231
Physical Location & Seq
Forward (+)
39735385 .. 39741856
6472 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g54430.t1

Sequence Viewer

Length: 2403 bp
ATGGCTCTCCTCCTTCCAATCCCTTCTCCGTCTTCTTCTTCTACTTGTTCTTCTTCTTATTATAGGCCGGCGGCAAGTCGGGTGTGCAGGGTCTGGGGAGTGTTGAAAATCCACAGCAGCCTCGGCCTTTCGGATTCCGTTTCCACCAGAAGCAAACCCCGGAAAAAGCGACGGGAGGCGGCAGTGGAGGCAGAGTCCGACGCCATATCGATTCTGAACGAGAGAATTCGGCGGGAGCAGAGAAAGGAATCGCCCCCAACCCCGATGGAGGCGGCGGAGGCTGACAAGTACTTGGAGCTGGTCAAGCAGCAGCAGCAGAGGGGCCTCCAGAAGCTCAAAGGCGATCTTAGTTACAAGGTCGATCCCTACACTCTTCGCTCCGGCGACTATGTTGTCCATAAGAAGGTCGGCATTGGTCGTTTCGTCGGCATTAAATTCGACAATGCTATTGAATACGTCTTCATCGAGTATGCTGATGGCATGGCTAAGCTTCCTGTCAAGCAGGCCTCTCGTCTGCTTTATCGTTACAATCTCCCCAACGAAAACAAAAAACCTCACACATTGAGCAAACTGAACGACACCAGTGTGTGGGAAAGAAGGAAGACCAAAGGAAAGATTGCCATTCAGAAAATGGTTGTTGATTTGATGGAGCTGTATTTACATAGGCTCAAACAAAGACGCCCTCCATACCCTCTTACTCCAGCCATGGCTCACTTTGTTTCTCAGTTTCCGTATGAGCCTACTCCTGATCAAAAGCAGGCTTTCATTGATGTTGACAAAGATTTAACAGGGAGAGAAACTCCAATGGACCGCTTAATTTGTGGAGATGTTGGCTTTGGCAAAACTGAAGTTGCCCTCCGTGCCATCTTTTGTGTTGTTTCTGCAGGGAAGCAAGCTATGGTTCTTGCCCCCACAATTGTTTTAGCCAAGCAACATTTTGAAGTTATCTCGCAACGTTTTTCTATATATCCAAATATCAATGTTGGACTTCTTAGCAGGTTTCAGACTAAATCAGAGAAAGATGAGCATCTGGACATGATAAAGAACGGCCATTTAGACATAATTGTCGGGACACACTCACTTCTTGGAAGCCGTGTTGTGTATAATAATCTAGGCCTGCTTGTTGTTGATGAAGAACAGAGGTTTGGCGTCAAACAGAAGGAGAAAATTGCTTCATTCAAAACATCAGTCGATGTTCTTACTCTATCCGCAACACCTATACCGCGAACCCTTTATTTAGCATTGACTGGTTTTCGTGATGCTAGCCTGATTTCAACTCCACCTCCAGAAAGGGTCCCTATAAGAACCCATCTCTCGGCATACAGTAAAGAAAAGGTATTATCAGCAATTAAGTATGAGCTGGACCGAGGTGGCCAAGTATTTTATGTTTTGCCTCGTATCAAAGGACTTGAAGAAGTCATGGATTTTCTTGAACAATCGTTTCCTGATGTTGAAATAGCTATTGCCCACGGGAAGCAATATTCAAAGCAGCTAGAGGAAACCATGGAGAAATTTTCACAAGGTGAAATCAAGATTCTTACATGCACTAATATAGTAGAAAGTGGGCTTGATATTCAGAATGCAAATACCATTATAATTCAGGACGTTCAACAATTTGGCCTAGCACAGTTGTATCAGCTTCGGGGAAGGGTGGGGAGGGCAGATAAGGAAGCTCATGCACACTTATTTTACCCAGAGAAGTCACTTCTAACTGATCAAGCGTTGGAGAGACTTGCAGCTCTTGAAGAATGCTGTCAACTTGGGCAAGGTTTCCAACTTGCAGAGAGGGACATGGGAATCAGAGGCTTTGGTACCATCTTTGGTGAGCAGCAGACTGGGGATGTTGGAAATGTTGGCATTGATCTCTTCTTTGAAATGCTGTTTGAGAGCTTGTCTAAGGTTGATGAGCATCGAGTAGTTTCAGTTCCTTACTGGTCTGTTGAGATTGATATGAATATAAACCCTCATCTCCCTTCTGAGTACATAAATAATCTCGAGAATCCCATGGAGATAATTCATGAAGCTGAGAGAGCTGCAGAGAAGGATATCTGGAGTTTGATGCAATATACTGAGAATTTGCGTCGCCAATACGGAAAGGAGCCACACTCCATGGAAATTCTACTAAAAAAGCTTTATGTGAGGAGAATGGCAGCAGATATTGGAATAACCAAAATATATGCTTCTGGAAAGATGGTTTTCATGAAAACAGTCATGAATAAACAGGTTTTCAAACTGATTACAGATTCAGTGGTTTCTGATGTTCACCGAAATTCTTTGGTATTTGAGGGTGACCAGATCAAGGCAGAACTTCTTCTAGAACTGCCGAGAGAACAACTCCTAAACTGGATATTTCAATGTTTGGCTGAACTACATGCTTCCTTACCTTCCCTCATAAAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarD_CdnL_TRCF DEAD Helicase_C TRCF

Protein Analysis

801

Amino Acids

90.84

Weight (kDa)

8.55

Isoelectric Point (pI)

35.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CarD_TRCF_RID PF02559 125 - 176 9.1e-11 CarD-like/TRCF RID domain
ResIII PF04851 246 - 406 1e-08 Type III restriction enzyme, res subunit
DEAD PF00270 248 - 410 2.2e-23 DEAD/DEAH box helicase
Helicase_C PF00271 448 - 554 2.4e-20 Helicase conserved C-terminal domain
TRCF PF03461 652 - 740 1.4e-06 TRCF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02060 AT3G02060 AT3G02060
fragaria_vesca FvH4_6g54430
malus_domestica MD17G1004500.v1.1
prunus_persica Prupe.3G314900_v2.0.a1
pyrus_communis pycom111g00110 pycom17g00110
rosa_chinensis RchiOBHm_Chr2g0176681
rosa_laevigata RLG00000022435
rosa_rugosa Rorug02G0597500
rosa_samantha Rh2BG686000 Rh2CG649300 Rh2DG700200
rosa_wichuraiana Rw0G015680 Rw2G055390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1595
AasI GACNNNNNNGTC 2 cut(s) 392, 1064
Acc36I ACCTGC 1 cut(s) 987
Acc65I GGTACC 1 cut(s) 1811
AccB1I GGYRCC 1 cut(s) 1811
AccB7I CCANNNNNTGG 1 cut(s) 588
AccII CGCG 1 cut(s) 1225
AciI CCGC 8 cut(s) 71, 179, 232, 272, 275, 811, 1209, 1223
AclI AACGTT 1 cut(s) 955
AclWI GGATC 1 cut(s) 356
AcoI YGGCCR 2 cut(s) 1048, 1372
AcsI RAATTY 6 cut(s) 225, 434, 1511, 2074, 2115, 2269
AcuI CTGAAG 1 cut(s) 867
AcyI GRCGYC 3 cut(s) 201, 679, 1149
AdeI CACNNNGTG 1 cut(s) 1523
AfaI GTAC 3 cut(s) 290, 1813, 1982
AfiI CCNNNNNNNGG 5 cut(s) 268, 403, 588, 1315, 2299
AjuI GAANNNNNNNTTGG 2 cut(s) 1128, 1160
AleI CACNNNNGTG 1 cut(s) 584
Alw26I GTCTC 1 cut(s) 1723
AlwI GGATC 1 cut(s) 356
AlwNI CAGNNNCTG 1 cut(s) 93
Ama87I CYCGRG 1 cut(s) 1994
AoxI GGCC 8 cut(s) 65, 124, 322, 504, 1048, 1114, 1372, 1618
ApeKI GCWGC 9 cut(s) 117, 307, 310, 313, 1489, 1736, 1828, 2033, 2150
ApoI RAATTY 6 cut(s) 225, 434, 1511, 2074, 2115, 2269
ArsI GACNNNNNNTTYG 2 cut(s) 1173, 1205
Asp700I GAANNNNTTC 1 cut(s) 2310
Asp718I GGTACC 1 cut(s) 1811
AspS9I GGNCC 4 cut(s) 322, 808, 1294, 1363
AsuC2I CCSGG 1 cut(s) 160
AsuHPI GGTGA 4 cut(s) 1535, 1835, 2255, 2300
AsuNHI GCTAGC 1 cut(s) 1262
AvaI CYCGRG 1 cut(s) 1994
AvaII GGWCC 3 cut(s) 808, 1294, 1363
BaeI ACNNNNGTAYC 2 cut(s) 1616, 1649
BalI TGGCCA 1 cut(s) 1374
BanI GGYRCC 1 cut(s) 1811
BbsI GAAGAC 3 cut(s) 24, 451, 608
BbvI GCAGC 9 cut(s) 129, 319, 322, 325, 1501, 1748, 1840, 2020, 2162
BccI CCATC 7 cut(s) 259, 470, 640, 872, 1317, 1823, 2185
BceAI ACGGC 2 cut(s) 1063, 1077
BcgI CGANNNNNNTGC 4 cut(s) 418, 452, 491, 525
BclI TGATCA 2 cut(s) 748, 1714
BcnI CCSGG 1 cut(s) 160
BcoDI GTCTC 1 cut(s) 1723
BfaI CTAG 5 cut(s) 1112, 1263, 1493, 1622, 2315
BfmI CTRYAG 2 cut(s) 882, 2034
BfuAI ACCTGC 1 cut(s) 987
BlpI GCTNAGC 1 cut(s) 486
BmcAI AGTACT 1 cut(s) 290
Bme1390I CCNGG 1 cut(s) 160
Bme18I GGWCC 3 cut(s) 808, 1294, 1363
BmeT110I CYCGRG 1 cut(s) 1994
BmgT120I GGNCC 4 cut(s) 322, 808, 1294, 1363
BmiI GGNNCC 5 cut(s) 323, 1295, 1296, 1813, 2100
BmrFI CCNGG 1 cut(s) 160
BmrI ACTGGG 1 cut(s) 1845
BmsI GCATC 4 cut(s) 1036, 1249, 1918, 2049
BmtI GCTAGC 1 cut(s) 1266
BmuI ACTGGG 1 cut(s) 1845
BpiI GAAGAC 3 cut(s) 24, 451, 608
BplI GAGNNNNNCTC 6 cut(s) 784, 816, 2090, 2122, 2319, 2351
BpmI CTGGAG 4 cut(s) 311, 684, 1269, 2071
Bpu1102I GCTNAGC 1 cut(s) 486
BpuMI CCSGG 1 cut(s) 160
Bsa29I ATCGAT 1 cut(s) 209
BsaBI GATNNNNATC 2 cut(s) 1026, 1908
BsaHI GRCGYC 3 cut(s) 201, 679, 1149
BsaJI CCNNGG 8 cut(s) 121, 158, 705, 1366, 1468, 1503, 2004, 2109
BsaXI ACNNNNNCTCC 4 cut(s) 179, 209, 1267, 1297
Bsc4I CCNNNNNNNGG 5 cut(s) 268, 403, 588, 1315, 2299
Bse118I RCCGGY 1 cut(s) 67
Bse1I ACTGG 5 cut(s) 582, 1252, 1840, 1937, 2348
Bse8I GATNNNNATC 2 cut(s) 1026, 1908
BseCI ATCGAT 1 cut(s) 209
BseDI CCNNGG 8 cut(s) 121, 158, 705, 1366, 1468, 1503, 2004, 2109
BseGI GGATG 1 cut(s) 1846
BseJI GATNNNNATC 2 cut(s) 1026, 1908
BseLI CCNNNNNNNGG 5 cut(s) 268, 403, 588, 1315, 2299
BseMII CTCAG 4 cut(s) 737, 1968, 2016, 2061
BseNI ACTGG 5 cut(s) 582, 1252, 1840, 1937, 2348
BseRI GAGGAG 1 cut(s) 2155
BseXI GCAGC 9 cut(s) 129, 319, 322, 325, 1501, 1748, 1840, 2020, 2162
BsgI GTGCAG 1 cut(s) 106
Bsh1236I CGCG 1 cut(s) 1225
BshFI GGCC 8 cut(s) 67, 126, 324, 506, 1050, 1116, 1374, 1620
BshNI GGYRCC 1 cut(s) 1811
BshVI ATCGAT 1 cut(s) 209
BsiHKCI CYCGRG 1 cut(s) 1994
BsiSI CCGG 3 cut(s) 68, 160, 381
BslFI GGGAC 3 cut(s) 1084, 1280, 1802
BslI CCNNNNNNNGG 5 cut(s) 268, 403, 588, 1315, 2299
BsmAI GTCTC 1 cut(s) 1723
BsmFI GGGAC 3 cut(s) 1084, 1280, 1802
BsmI GAATGC 2 cut(s) 1585, 1754
BsnI GGCC 8 cut(s) 67, 126, 324, 506, 1050, 1116, 1374, 1620
BsoBI CYCGRG 1 cut(s) 1994
Bsp143I GATC 6 cut(s) 343, 361, 748, 1714, 1861, 2295
Bsp1720I GCTNAGC 1 cut(s) 486
Bsp19I CCATGG 4 cut(s) 705, 1503, 2004, 2109
BspACI CCGC 8 cut(s) 71, 179, 232, 272, 275, 811, 1209, 1223
BspANI GGCC 8 cut(s) 67, 126, 324, 506, 1050, 1116, 1374, 1620
BspCNI CTCAG 4 cut(s) 736, 1969, 2017, 2062
BspDI ATCGAT 1 cut(s) 209
BspFNI CGCG 1 cut(s) 1225
BspHI TCATGA 3 cut(s) 2017, 2199, 2211
BspLI GGNNCC 5 cut(s) 323, 1295, 1296, 1813, 2100
BspMAI CTGCAG 2 cut(s) 886, 2038
BspMI ACCTGC 1 cut(s) 987
BspOI GCTAGC 1 cut(s) 1266
BspPI GGATC 1 cut(s) 356
BspT107I GGYRCC 1 cut(s) 1811
BsrFI RCCGGY 1 cut(s) 67
BsrI ACTGG 5 cut(s) 582, 1252, 1840, 1937, 2348
BssAI RCCGGY 1 cut(s) 67
BssECI CCNNGG 8 cut(s) 121, 158, 705, 1366, 1468, 1503, 2004, 2109
BssMI GATC 6 cut(s) 343, 361, 748, 1714, 1861, 2295
BssNI GRCGYC 3 cut(s) 201, 679, 1149
BssT1I CCWWGG 4 cut(s) 705, 1503, 2004, 2109
Bst4CI ACNGT 3 cut(s) 1325, 1629, 2209
Bst6I CTCTTC 2 cut(s) 378, 1871
BstACI GRCGYC 3 cut(s) 201, 679, 1149
BstC8I GCNNGC 6 cut(s) 69, 504, 759, 894, 1118, 1264
BstDEI CTNAG 8 cut(s) 347, 486, 723, 992, 1896, 1977, 2025, 2070
BstDSI CCRYGG 5 cut(s) 705, 1468, 1503, 2004, 2109
BstEII GGTNACC 1 cut(s) 2288
BstF5I GGATG 1 cut(s) 1846
BstFNI CGCG 1 cut(s) 1225
BstKTI GATC 6 cut(s) 346, 364, 751, 1717, 1864, 2298
BstMAI GTCTC 1 cut(s) 1723
BstMBI GATC 6 cut(s) 343, 361, 748, 1714, 1861, 2295
BstMWI GCNNNNNNNGC 7 cut(s) 123, 188, 278, 304, 313, 860, 2030
BstNSI RCATGY 2 cut(s) 1545, 2375
BstPI GGTNACC 1 cut(s) 2288
BstSCI CCNGG 1 cut(s) 158
BstSFI CTRYAG 2 cut(s) 882, 2034
BstUI CGCG 1 cut(s) 1225
BstV1I GCAGC 9 cut(s) 129, 319, 322, 325, 1501, 1748, 1840, 2020, 2162
BstV2I GAAGAC 3 cut(s) 24, 451, 608
Bsu15I ATCGAT 1 cut(s) 209
BsuRI GGCC 8 cut(s) 67, 126, 324, 506, 1050, 1116, 1374, 1620
BsuTUI ATCGAT 1 cut(s) 209
BtgI CCRYGG 5 cut(s) 705, 1468, 1503, 2004, 2109
BtsCI GGATG 1 cut(s) 1846
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 3 cut(s) 189, 589, 2253
BveI ACCTGC 1 cut(s) 987
Cac8I GCNNGC 6 cut(s) 69, 504, 759, 894, 1118, 1264
CaiI CAGNNNCTG 1 cut(s) 93
CciI TCATGA 3 cut(s) 2017, 2199, 2211
Cfr10I RCCGGY 1 cut(s) 67
Cfr13I GGNCC 4 cut(s) 322, 808, 1294, 1363
ClaI ATCGAT 1 cut(s) 209
CseI GACGC 4 cut(s) 209, 687, 1138, 2069
Csp6I GTAC 3 cut(s) 289, 1812, 1981
CviQI GTAC 3 cut(s) 289, 1812, 1981
DdeI CTNAG 8 cut(s) 347, 486, 723, 992, 1896, 1977, 2025, 2070
DpnI GATC 6 cut(s) 345, 363, 750, 1716, 1863, 2297
DpnII GATC 6 cut(s) 343, 361, 748, 1714, 1861, 2295
DraIII CACNNNGTG 1 cut(s) 1523
DrdI GACNNNNNNGTC 2 cut(s) 392, 1064
DseDI GACNNNNNNGTC 2 cut(s) 392, 1064
EaeI YGGCCR 2 cut(s) 1048, 1372
Eam1104I CTCTTC 2 cut(s) 378, 1871
EarI CTCTTC 2 cut(s) 378, 1871
EciI GGCGGA 1 cut(s) 290
Eco130I CCWWGG 4 cut(s) 705, 1503, 2004, 2109
Eco147I AGGCCT 2 cut(s) 506, 1116
Eco32I GATATC 1 cut(s) 2047
Eco47I GGWCC 3 cut(s) 808, 1294, 1363
Eco57I CTGAAG 1 cut(s) 867
Eco88I CYCGRG 1 cut(s) 1994
Eco91I GGTNACC 1 cut(s) 2288
EcoO109I RGGNCCY 2 cut(s) 322, 1294
EcoO65I GGTNACC 1 cut(s) 2288
EcoRI GAATTC 1 cut(s) 225
EcoRV GATATC 1 cut(s) 2047
EcoT14I CCWWGG 4 cut(s) 705, 1503, 2004, 2109
ErhI CCWWGG 4 cut(s) 705, 1503, 2004, 2109
FalI AAGNNNNNCTT 2 cut(s) 330, 362
FaqI GGGAC 3 cut(s) 1084, 1280, 1802
FauI CCCGC 1 cut(s) 225
FbaI TGATCA 2 cut(s) 748, 1714
FokI GGATG 1 cut(s) 1853
FspBI CTAG 5 cut(s) 1112, 1263, 1493, 1622, 2315
GsuI CTGGAG 4 cut(s) 311, 684, 1269, 2071
HaeIII GGCC 8 cut(s) 67, 126, 324, 506, 1050, 1116, 1374, 1620
HapII CCGG 3 cut(s) 68, 160, 381
HgaI GACGC 4 cut(s) 209, 687, 1138, 2069
Hin1I GRCGYC 3 cut(s) 201, 679, 1149
HincII GTYRAC 2 cut(s) 775, 1757
HindII GTYRAC 2 cut(s) 775, 1757
HindIII AAGCTT 2 cut(s) 488, 2129
HinfI GANTC 8 cut(s) 134, 194, 211, 248, 1534, 1797, 1999, 2243
HpaII CCGG 3 cut(s) 68, 160, 381
HphI GGTGA 4 cut(s) 1535, 1835, 2255, 2300
Hpy166II GTNNAC 3 cut(s) 775, 1757, 2263
Hpy8I GTNNAC 3 cut(s) 775, 1757, 2263
Hpy99I CGWCG 4 cut(s) 174, 203, 428, 2085
HpyAV CCTTC 9 cut(s) 23, 33, 397, 591, 1153, 1641, 1983, 2035, 2394
HpyCH4III ACNGT 3 cut(s) 1325, 1629, 2209
HpyCH4IV ACGT 3 cut(s) 456, 955, 1605
HpyCH4V TGCA 9 cut(s) 87, 884, 1545, 1583, 1679, 1736, 1781, 2036, 2062
HpyF10VI GCNNNNNNNGC 7 cut(s) 123, 188, 278, 304, 313, 860, 2030
HpyF3I CTNAG 8 cut(s) 347, 486, 723, 992, 1896, 1977, 2025, 2070
HpySE526I ACGT 3 cut(s) 456, 955, 1605
Hsp92I GRCGYC 3 cut(s) 201, 679, 1149
KflI GGGWCCC 1 cut(s) 1294
KpnI GGTACC 1 cut(s) 1815
KroI GCCGGC 1 cut(s) 67
KroNI GCCGGC 1 cut(s) 69
Ksp22I TGATCA 2 cut(s) 748, 1714
Kzo9I GATC 6 cut(s) 343, 361, 748, 1714, 1861, 2295
LmnI GCTCC 5 cut(s) 235, 295, 383, 649, 2098
Lsp1109I GCAGC 9 cut(s) 129, 319, 322, 325, 1501, 1748, 1840, 2020, 2162
LweI GCATC 4 cut(s) 1036, 1249, 1918, 2049
MaeI CTAG 5 cut(s) 1112, 1263, 1493, 1622, 2315
MaeII ACGT 3 cut(s) 456, 955, 1605
MaeIII GTNAC 4 cut(s) 350, 524, 1701, 2288
MalI GATC 6 cut(s) 345, 363, 750, 1716, 1863, 2297
MboI GATC 6 cut(s) 343, 361, 748, 1714, 1861, 2295
MfeI CAATTG 1 cut(s) 915
MlsI TGGCCA 1 cut(s) 1374
MluNI TGGCCA 1 cut(s) 1374
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 5 cut(s) 222, 964, 1704, 1798, 1825
Mox20I TGGCCA 1 cut(s) 1374
MroNI GCCGGC 1 cut(s) 67
MroXI GAANNNNTTC 1 cut(s) 2310
MscI TGGCCA 1 cut(s) 1374
MseI TTAA 4 cut(s) 432, 785, 815, 1350
MslI CAYNNNNRTG 1 cut(s) 584
Msp20I TGGCCA 1 cut(s) 1374
MspI CCGG 3 cut(s) 68, 160, 381
MspR9I CCNGG 1 cut(s) 160
MunI CAATTG 1 cut(s) 915
Mva1269I GAATGC 2 cut(s) 1585, 1754
MvnI CGCG 1 cut(s) 1225
MwoI GCNNNNNNNGC 7 cut(s) 123, 188, 278, 304, 313, 860, 2030
NaeI GCCGGC 1 cut(s) 69
NciI CCSGG 1 cut(s) 160
NcoI CCATGG 4 cut(s) 705, 1503, 2004, 2109
NdeII GATC 6 cut(s) 343, 361, 748, 1714, 1861, 2295
NgoMIV GCCGGC 1 cut(s) 67
NheI GCTAGC 1 cut(s) 1262
NlaIV GGNNCC 5 cut(s) 323, 1295, 1296, 1813, 2100
NmeAIII GCCGAG 3 cut(s) 102, 1295, 2349
NmuCI GTSAC 2 cut(s) 1701, 2288
NspI RCATGY 2 cut(s) 1545, 2375
OliI CACNNNNGTG 1 cut(s) 584
PaeR7I CTCGAG 1 cut(s) 1994
PagI TCATGA 3 cut(s) 2017, 2199, 2211
PceI AGGCCT 2 cut(s) 506, 1116
PcsI WCGNNNNNNNCGW 1 cut(s) 462
PctI GAATGC 2 cut(s) 1585, 1754
PdiI GCCGGC 1 cut(s) 69
PdmI GAANNNNTTC 1 cut(s) 2310
PfeI GAWTC 7 cut(s) 134, 211, 248, 1534, 1797, 1999, 2243
PflMI CCANNNNNTGG 1 cut(s) 588
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
PpuMI RGGWCCY 1 cut(s) 1294
PsiI TTATAA 1 cut(s) 1595
Psp1406I AACGTT 1 cut(s) 955
Psp5II RGGWCCY 1 cut(s) 1294
PspEI GGTNACC 1 cut(s) 2288
PspN4I GGNNCC 5 cut(s) 323, 1295, 1296, 1813, 2100
PspPI GGNCC 4 cut(s) 322, 808, 1294, 1363
PspPPI RGGWCCY 1 cut(s) 1294
PsrI GAACNNNNNNTAC 2 cut(s) 1908, 1940
PstI CTGCAG 2 cut(s) 886, 2038
PstNI CAGNNNCTG 1 cut(s) 93
RsaI GTAC 3 cut(s) 290, 1813, 1982
RsaNI GTAC 3 cut(s) 289, 1812, 1981
RseI CAYNNNNRTG 1 cut(s) 584
SaqAI TTAA 4 cut(s) 432, 785, 815, 1350
Sau3AI GATC 6 cut(s) 343, 361, 748, 1714, 1861, 2295
Sau96I GGNCC 4 cut(s) 322, 808, 1294, 1363
ScaI AGTACT 1 cut(s) 290
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 1 cut(s) 160
SfaNI GCATC 4 cut(s) 1036, 1249, 1918, 2049
SfcI CTRYAG 2 cut(s) 882, 2034
Sfr274I CTCGAG 1 cut(s) 1994
SinI GGWCC 3 cut(s) 808, 1294, 1363
SlaI CTCGAG 1 cut(s) 1994
SmiMI CAYNNNNRTG 1 cut(s) 584
SmlI CTYRAG 1 cut(s) 1994
SmoI CTYRAG 1 cut(s) 1994
SseBI AGGCCT 2 cut(s) 506, 1116
SsiI CCGC 8 cut(s) 71, 179, 232, 272, 275, 811, 1209, 1223
SspI AATATT 1 cut(s) 1481
SspMI CTAG 5 cut(s) 1112, 1263, 1493, 1622, 2315
StuI AGGCCT 2 cut(s) 506, 1116
StyD4I CCNGG 1 cut(s) 158
StyI CCWWGG 4 cut(s) 705, 1503, 2004, 2109
TaaI ACNGT 3 cut(s) 1325, 1629, 2209
TaiI ACGT 3 cut(s) 459, 958, 1608
TaqI TCGA 7 cut(s) 209, 360, 438, 465, 1191, 1912, 1995
TaqII GACCGA 1 cut(s) 1380
TatI WGTACW 2 cut(s) 288, 1980
TauI GCSGC 3 cut(s) 74, 182, 275
TfiI GAWTC 7 cut(s) 134, 211, 248, 1534, 1797, 1999, 2243
Tru1I TTAA 4 cut(s) 432, 785, 815, 1350
Tru9I TTAA 4 cut(s) 432, 785, 815, 1350
TscAI CASTG 3 cut(s) 189, 589, 2253
TseFI GTSAC 2 cut(s) 1701, 2288
TseI GCWGC 9 cut(s) 117, 307, 310, 313, 1489, 1736, 1828, 2033, 2150
Tsp45I GTSAC 2 cut(s) 1701, 2288
TspGWI ACGGA 5 cut(s) 18, 127, 720, 848, 2106
TspRI CASTG 3 cut(s) 189, 589, 2253
Van91I CCANNNNNTGG 1 cut(s) 588
VpaK11BI GGWCC 3 cut(s) 808, 1294, 1363
XapI RAATTY 6 cut(s) 225, 434, 1511, 2074, 2115, 2269
XbaI TCTAGA 1 cut(s) 2314
XceI RCATGY 2 cut(s) 1545, 2375
XcmI CCANNNNNNNNNTGG 1 cut(s) 628
XhoI CTCGAG 1 cut(s) 1994
XmnI GAANNNNTTC 1 cut(s) 2310
XspI CTAG 5 cut(s) 1112, 1263, 1493, 1622, 2315
ZrmI AGTACT 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.