MD17G1004500.v1.1

CarD-like/TRCF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015231
Physical Location & Seq
Reverse (-)
364529 .. 369599
5071 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1004500.v1.1.491

Sequence Viewer

Length: 2481 bp
ATGGCGTTGCTCCTCCCAATCCCCGCCCTAAACTCTAGCTCGGCAATTCGAGGGCTGAGGGTGCCGCCGTGTCCCGGGGCCAACTTTTGTTGCAGAGTCGGACTCGGAAGAAGAGGACTGGTTTGCTGGGTGCCCAACGCTGTCTACACCGCCCAGACTCACACCCACACCCAAAGCACGGAACAGCAGTGGAAACCTAAATCGGGGAAGAGAGAGGATTCGGCAGAGGGCGACGACATATCCATTCTGAACGAGAGGATTCGGCGGGAGCAAAGCAAGAGAGAGGGCTCGGGAACGAGAACGGTGATGGAATCGGAGGAGGCGGATAAGTACATAGAGATGGTGAAGCAGCAGCAGCAGAGGGGCCTCGAGAAGCTCCGAGGCGATGTTGGTTACAAGGTGGACCCCTACACTCTTCGTTCTGGGGACTACGTCGTCCATAAGAAGGTCGGCATTGGTCGATTCGTTGGGATTAAGTTAGACAGTACTAGTCCCAATTTCAATTCCGCTTCCAAGACTGACTCCATTGAATACGTCTTCATCGAGTATGCCGATGGCATGGCTAAGCTTCCTGTCAAGCAGGCCTCTCGCTTGCTCTATCGTTATAATCTGCCGAACGAAACGAAAAGGCCTCGCACGTTGAGCAAATTGAGTGACACCAGTGTCTGGGAGAAAAGAAAGACCAAAGGAAAGATTGCCATTCAGAAAATGGTTGTTGATTTGATGGAGCTGTATCTTCACAGACTCAAACAAAGACGCCCTCCCTACCCACTCACTTCTGCCATGACTCACTTTGTTTCTCAGTTTCCTTATCAACCCACCCCTGATCAACAGCAGGCTTTCATTGATGTTCACAAAGATCTAACCGCCAGAGAAACCCCAATGGACCGCTTAATTTGTGGAGATGTTGGCTTTGGCAAAACCGAAGTTGCCCTGCGTGCCATCTTCTGTGTAGTTTCTGCAGGGAAGCAAGCTATGGTTCTCGCACCCACTATTGTTTTGGCCAAGCAACATTTTGATGTTATTGCAGACCGGTTTTCTATCTACCCCGACGTCAAGGTTGGACTTCTCAGCAGGTTTCAGACCAAAGCAGAAAAGGAGGGGCATTTGGACATGATTAAAAAGGGGCAATTGGACATAATTGTTGGGACTCACTCGCTTCTTGGAAGTCGTGTTGTGTATAGTAATCTGGGTTTGTTAGTGGTTGATGAAGAACAGAGGTTTGGCGTCAAACAAAAGGAGAAGATTGCTTCTTTCAAAACATCGGTTGATGTTCTTACTCTTTCTGCAACACCTATACCACGAACCCTGTATTTAGCCTTGACTGGGTTCCGTGATGTTAGTTTGATTTCAACTCCACCACCAGAAAGGGTCCCTATCAAATCCCATCTTTCTGCATACAGTAAAGAAAAGGTTTTATCAGCAATTAAGCATGAGCTGGACAGAGGAGGCCAAGTGTTTTATGTATTGCCTCGCATTAAAGGACTTGAAGAAGTGATGGACTTTCTTGAACAGTCATTTCCTAATGTTGAAATAGCTATTGCACATGGGAAGCAATATTCAAAGCAGCTGGAGGAAACCATGGAGAATTTTGCACAAGGTGAAATCAAGATTCTTATATGCACTAATATAGTAGAAAGTGGGCTTGACATTCAGAATGCAAACACCATCATAATTCAGGATGTTCAACAATTTGGCTTAGCGCAGTTGTACCAGTTGCGGGGAAGGGTAGGCCGGGCAGATAAGGAAGCTCATGCATACTTGTTCTATCCAGAGAAGTCACTGCTAACTGATCAAGCGCTGGAGCGACTTGCAGCTCTTGAGGAATGCTGTGAACTTGGGCAAGGTTTCCAACTTGCAGAGAAGGACATGGGAATCAGAGGCTTTGGTACCATATTTGGTGAGCAACAAACAGGGGATGTTGGAAATGTTGGCATTGATCTCTTCTTTGAAATGCTCTTTGAGAGCCTATCCAAGGTTGATGAACACCGTGTAGTTTCAGTTCCTTACTGGTCCGTTGAGATTGATTTAAATTTAAATCCTCATCTCCCCTCTGAATACATAAATTATCTGGAGAATCCCATGGAGATAATTCATGAAGCCGAGATAGCTGCTGAGAAAGATATCTGGAGTTTGATGCATTATGCTGAGAATTTACGCAGGCAATATGGAAAGGAGCCACACTCCATGGAGATTCTTTTAAAGAAGCTTTATGTGAGGAGGATGGCAGCAGATCTTGGAATAAGCAAGATATATGCCTCAGGAAAGATGGTTTTTATGAAAACAAGTATGAAGAAAAAGGTCTTCAAGCTGATTACCGAATCAATGGTCTCTGATGTCTACCGAAACTCTCTGGTTTTTGAGGGTGACCAGATCAAGGCGGAACTTCTTCTAGAGCTACCGAGGGAACAGCTGTTGAATTGGATCTTTCAATGTTTGGCTGAACTGCATGCTTCCTTACCTGCTCTCATAAAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarD_CdnL_TRCF DEAD Helicase_C TRCF

Protein Analysis

827

Amino Acids

93.46

Weight (kDa)

8.39

Isoelectric Point (pI)

34.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CarD_TRCF_RID PF02559 139 - 202 2.2e-10 CarD-like/TRCF RID domain
ResIII PF04851 272 - 432 3.9e-08 Type III restriction enzyme, res subunit
DEAD PF00270 274 - 436 1.7e-22 DEAD/DEAH box helicase
Helicase_C PF00271 475 - 580 4.3e-21 Helicase conserved C-terminal domain
TRCF PF03461 677 - 767 3.3e-06 TRCF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02060 AT3G02060 AT3G02060
fragaria_vesca FvH4_6g54430
malus_domestica MD17G1004500.v1.1
prunus_persica Prupe.3G314900_v2.0.a1
pyrus_communis pycom111g00110 pycom17g00110
rosa_chinensis RchiOBHm_Chr2g0176681
rosa_laevigata RLG00000022435
rosa_rugosa Rorug02G0597500
rosa_samantha Rh2BG686000 Rh2CG649300 Rh2DG700200
rosa_wichuraiana Rw0G015680 Rw2G055390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 606
AasI GACNNNNNNGTC 2 cut(s) 434, 662
AatII GACGTC 1 cut(s) 1056
Acc36I ACCTGC 2 cut(s) 1065, 2470
Acc65I GGTACC 1 cut(s) 1889
AccB1I GGYRCC 3 cut(s) 61, 130, 1889
AccB7I CCANNNNNTGG 1 cut(s) 666
AccI GTMKAC 2 cut(s) 144, 2340
AclWI GGATC 1 cut(s) 2432
AcoI YGGCCR 1 cut(s) 1002
AcsI RAATTY 3 cut(s) 1588, 2032, 2152
AcyI GRCGYC 3 cut(s) 757, 1053, 1227
AdeI CACNNNGTG 1 cut(s) 1601
AfaI GTAC 4 cut(s) 332, 487, 1712, 1891
AfeI AGCGCT 1 cut(s) 1800
AfiI CCNNNNNNNGG 9 cut(s) 74, 178, 203, 445, 666, 1326, 1720, 1975, 2377
AgeI ACCGGT 1 cut(s) 1032
AhlI ACTAGT 1 cut(s) 488
AjuI GAANNNNNNNTTGG 2 cut(s) 1206, 1238
Alw26I GTCTC 1 cut(s) 2335
AlwI GGATC 1 cut(s) 2432
AlwNI CAGNNNCTG 1 cut(s) 666
Ama87I CYCGRG 3 cut(s) 74, 289, 368
Aor51HI AGCGCT 1 cut(s) 1800
AoxI GGCC 7 cut(s) 78, 364, 582, 629, 1002, 1450, 1732
ApeKI GCWGC 7 cut(s) 349, 352, 355, 1567, 1814, 2111, 2228
ApoI RAATTY 3 cut(s) 1588, 2032, 2152
ArsI GACNNNNNNTTYG 2 cut(s) 2313, 2345
AsiGI ACCGGT 1 cut(s) 1032
Asp700I GAANNNNTTC 1 cut(s) 2388
Asp718I GGTACC 1 cut(s) 1889
AspLEI GCGC 2 cut(s) 1705, 1801
AspS9I GGNCC 6 cut(s) 78, 364, 403, 886, 1372, 2013
AsuC2I CCSGG 3 cut(s) 75, 76, 1736
AsuHPI GGTGA 5 cut(s) 316, 355, 1613, 1913, 2378
AvaI CYCGRG 3 cut(s) 74, 289, 368
AvaII GGWCC 4 cut(s) 403, 886, 1372, 2013
AxyI CCTNAGG 1 cut(s) 2260
BaeGI GKGCMC 1 cut(s) 135
BalI TGGCCA 1 cut(s) 1004
BanI GGYRCC 3 cut(s) 61, 130, 1889
BanII GRGCYC 1 cut(s) 290
BbsI GAAGAC 2 cut(s) 529, 2296
BbvCI CCTCAGC 1 cut(s) 56
BbvI GCAGC 7 cut(s) 361, 364, 367, 1579, 1826, 2098, 2240
BceAI ACGGC 1 cut(s) 52
BcgI CGANNNNNNTGC 4 cut(s) 569, 603, 2093, 2127
BclI TGATCA 2 cut(s) 826, 1792
BcnI CCSGG 3 cut(s) 75, 76, 1736
BcoDI GTCTC 1 cut(s) 2335
BcuI ACTAGT 1 cut(s) 488
BfaI CTAG 3 cut(s) 36, 489, 2393
BfmI CTRYAG 1 cut(s) 960
BfoI RGCGCY 1 cut(s) 1802
BfuAI ACCTGC 2 cut(s) 1065, 2470
BglII AGATCT 2 cut(s) 859, 2233
BisI GCNGC 8 cut(s) 65, 350, 353, 356, 1568, 1815, 2112, 2229
BlpI GCTNAGC 2 cut(s) 564, 1699
BlsI GCNGC 8 cut(s) 66, 351, 354, 357, 1569, 1816, 2113, 2230
BmcAI AGTACT 1 cut(s) 487
Bme1390I CCNGG 3 cut(s) 75, 76, 1736
Bme18I GGWCC 4 cut(s) 403, 886, 1372, 2013
BmeT110I CYCGRG 3 cut(s) 74, 289, 368
BmgT120I GGNCC 6 cut(s) 78, 364, 403, 886, 1372, 2013
BmrFI CCNGG 3 cut(s) 75, 76, 1736
BmrI ACTGGG 1 cut(s) 1335
BmsI GCATC 1 cut(s) 2127
BmuI ACTGGG 1 cut(s) 1335
BpiI GAAGAC 2 cut(s) 529, 2296
BplI GAGNNNNNCTC 4 cut(s) 87, 119, 2168, 2200
BpmI CTGGAG 4 cut(s) 1592, 1823, 2093, 2149
Bpu10I CCTNAGC 1 cut(s) 56
Bpu1102I GCTNAGC 2 cut(s) 564, 1699
BpuEI CTTGAG 1 cut(s) 1841
BpuMI CCSGG 3 cut(s) 75, 76, 1736
BsaHI GRCGYC 3 cut(s) 757, 1053, 1227
BsaI GGTCTC 1 cut(s) 2335
BsaJI CCNNGG 8 cut(s) 74, 75, 379, 1581, 1974, 2082, 2187, 2402
BsaWI WCCGGW 1 cut(s) 1032
BsaXI ACNNNNNCTCC 2 cut(s) 1440, 1470
Bsc4I CCNNNNNNNGG 9 cut(s) 74, 178, 203, 445, 666, 1326, 1720, 1975, 2377
Bse118I RCCGGY 1 cut(s) 1032
Bse1I ACTGG 5 cut(s) 123, 660, 1330, 1714, 2015
Bse21I CCTNAGG 1 cut(s) 2260
BseDI CCNNGG 8 cut(s) 74, 75, 379, 1581, 1974, 2082, 2187, 2402
BseGI GGATG 3 cut(s) 1687, 1924, 2229
BseLI CCNNNNNNNGG 9 cut(s) 74, 178, 203, 445, 666, 1326, 1720, 1975, 2377
BseMII CTCAG 6 cut(s) 47, 815, 1084, 2106, 2139, 2274
BseNI ACTGG 5 cut(s) 123, 660, 1330, 1714, 2015
BseRI GAGGAG 3 cut(s) 332, 1461, 2233
BseSI GKGCMC 1 cut(s) 135
BseXI GCAGC 7 cut(s) 361, 364, 367, 1579, 1826, 2098, 2240
BseYI CCCAGC 1 cut(s) 126
BshFI GGCC 7 cut(s) 80, 366, 584, 631, 1004, 1452, 1734
BshNI GGYRCC 3 cut(s) 61, 130, 1889
BshTI ACCGGT 1 cut(s) 1032
BsiHKCI CYCGRG 3 cut(s) 74, 289, 368
BsiSI CCGG 3 cut(s) 75, 1033, 1735
BslFI GGGAC 5 cut(s) 57, 440, 477, 1162, 1358
BslI CCNNNNNNNGG 9 cut(s) 74, 178, 203, 445, 666, 1326, 1720, 1975, 2377
BsmAI GTCTC 1 cut(s) 2335
BsmFI GGGAC 5 cut(s) 57, 440, 477, 1162, 1358
BsmI GAATGC 2 cut(s) 1663, 1832
BsnI GGCC 7 cut(s) 80, 366, 584, 631, 1004, 1452, 1734
Bso31I GGTCTC 1 cut(s) 2335
BsoBI CYCGRG 3 cut(s) 74, 289, 368
Bsp1286I GDGCHC 2 cut(s) 135, 290
Bsp143I GATC 7 cut(s) 826, 859, 1792, 1939, 2233, 2373, 2424
Bsp1720I GCTNAGC 2 cut(s) 564, 1699
Bsp19I CCATGG 3 cut(s) 1581, 2082, 2187
BspANI GGCC 7 cut(s) 80, 366, 584, 631, 1004, 1452, 1734
BspCNI CTCAG 6 cut(s) 48, 814, 1083, 2107, 2140, 2273
BspHI TCATGA 1 cut(s) 2095
BspMAI CTGCAG 1 cut(s) 964
BspMI ACCTGC 2 cut(s) 1065, 2470
BspPI GGATC 1 cut(s) 2432
BspT107I GGYRCC 3 cut(s) 61, 130, 1889
BspTNI GGTCTC 1 cut(s) 2335
BsrFI RCCGGY 1 cut(s) 1032
BsrI ACTGG 5 cut(s) 123, 660, 1330, 1714, 2015
BssAI RCCGGY 1 cut(s) 1032
BssECI CCNNGG 8 cut(s) 74, 75, 379, 1581, 1974, 2082, 2187, 2402
BssMI GATC 7 cut(s) 826, 859, 1792, 1939, 2233, 2373, 2424
BssNI GRCGYC 3 cut(s) 757, 1053, 1227
BssT1I CCWWGG 4 cut(s) 1581, 1974, 2082, 2187
Bst4CI ACNGT 5 cut(s) 304, 485, 1403, 1515, 1991
Bst6I CTCTTC 4 cut(s) 106, 203, 420, 1949
BstACI GRCGYC 3 cut(s) 757, 1053, 1227
BstC8I GCNNGC 7 cut(s) 582, 593, 837, 939, 972, 2162, 2451
BstDEI CTNAG 8 cut(s) 56, 564, 801, 1070, 1699, 2115, 2148, 2260
BstDSI CCRYGG 3 cut(s) 1581, 2082, 2187
BstEII GGTNACC 1 cut(s) 2366
BstENI CCTNNNNNAGG 1 cut(s) 1973
BstF5I GGATG 3 cut(s) 1687, 1924, 2229
BstH2I RGCGCY 1 cut(s) 1802
BstHHI GCGC 2 cut(s) 1705, 1801
BstKTI GATC 7 cut(s) 829, 862, 1795, 1942, 2236, 2376, 2427
BstMAI GTCTC 1 cut(s) 2335
BstMBI GATC 7 cut(s) 826, 859, 1792, 1939, 2233, 2373, 2424
BstMWI GCNNNNNNNGC 5 cut(s) 61, 355, 642, 938, 2108
BstNSI RCATGY 1 cut(s) 2453
BstPI GGTNACC 1 cut(s) 2366
BstSCI CCNGG 3 cut(s) 73, 74, 1734
BstSFI CTRYAG 1 cut(s) 960
BstSLI GKGCMC 1 cut(s) 135
BstV1I GCAGC 7 cut(s) 361, 364, 367, 1579, 1826, 2098, 2240
BstV2I GAAGAC 2 cut(s) 529, 2296
BstX2I RGATCY 3 cut(s) 859, 2233, 2424
BstYI RGATCY 3 cut(s) 859, 2233, 2424
Bsu36I CCTNAGG 1 cut(s) 2260
BsuRI GGCC 7 cut(s) 80, 366, 584, 631, 1004, 1452, 1734
BtgI CCRYGG 3 cut(s) 1581, 2082, 2187
BtgZI GCGATG 1 cut(s) 399
BtsCI GGATG 3 cut(s) 1687, 1924, 2229
BtsI GCAGTG 2 cut(s) 194, 1781
BtsIMutI CAGTG 3 cut(s) 194, 667, 1781
BveI ACCTGC 2 cut(s) 1065, 2470
Cac8I GCNNGC 7 cut(s) 582, 593, 837, 939, 972, 2162, 2451
CaiI CAGNNNCTG 1 cut(s) 666
CciI TCATGA 1 cut(s) 2095
CfoI GCGC 2 cut(s) 1705, 1801
Cfr10I RCCGGY 1 cut(s) 1032
Cfr13I GGNCC 6 cut(s) 78, 364, 403, 886, 1372, 2013
Cfr9I CCCGGG 1 cut(s) 74
CseI GACGC 2 cut(s) 765, 1216
Csp6I GTAC 4 cut(s) 331, 486, 1711, 1890
CspAI ACCGGT 1 cut(s) 1032
CviQI GTAC 4 cut(s) 331, 486, 1711, 1890
DdeI CTNAG 8 cut(s) 56, 564, 801, 1070, 1699, 2115, 2148, 2260
DpnI GATC 7 cut(s) 828, 861, 1794, 1941, 2235, 2375, 2426
DpnII GATC 7 cut(s) 826, 859, 1792, 1939, 2233, 2373, 2424
DraI TTTAAA 3 cut(s) 2031, 2037, 2202
DraIII CACNNNGTG 1 cut(s) 1601
DrdI GACNNNNNNGTC 2 cut(s) 434, 662
DseDI GACNNNNNNGTC 2 cut(s) 434, 662
EaeI YGGCCR 1 cut(s) 1002
Eam1104I CTCTTC 4 cut(s) 106, 203, 420, 1949
EarI CTCTTC 4 cut(s) 106, 203, 420, 1949
EciI GGCGGA 2 cut(s) 338, 2396
Eco130I CCWWGG 4 cut(s) 1581, 1974, 2082, 2187
Eco147I AGGCCT 2 cut(s) 584, 631
Eco24I GRGCYC 1 cut(s) 290
Eco31I GGTCTC 1 cut(s) 2335
Eco32I GATATC 1 cut(s) 2125
Eco47I GGWCC 4 cut(s) 403, 886, 1372, 2013
Eco47III AGCGCT 1 cut(s) 1800
Eco81I CCTNAGG 1 cut(s) 2260
Eco88I CYCGRG 3 cut(s) 74, 289, 368
Eco91I GGTNACC 1 cut(s) 2366
EcoNI CCTNNNNNAGG 1 cut(s) 1973
EcoO109I RGGNCCY 2 cut(s) 364, 1372
EcoO65I GGTNACC 1 cut(s) 2366
EcoRV GATATC 1 cut(s) 2125
EcoT14I CCWWGG 4 cut(s) 1581, 1974, 2082, 2187
EcoT22I ATGCAT 2 cut(s) 1759, 2142
EcoT38I GRGCYC 1 cut(s) 290
ErhI CCWWGG 4 cut(s) 1581, 1974, 2082, 2187
FaqI GGGAC 5 cut(s) 57, 440, 477, 1162, 1358
FauI CCCGC 3 cut(s) 31, 258, 1713
FbaI TGATCA 2 cut(s) 826, 1792
FblI GTMKAC 2 cut(s) 144, 2340
Fnu4HI GCNGC 8 cut(s) 65, 350, 353, 356, 1568, 1815, 2112, 2229
FokI GGATG 3 cut(s) 1694, 1931, 2236
FriOI GRGCYC 1 cut(s) 290
Fsp4HI GCNGC 8 cut(s) 65, 350, 353, 356, 1568, 1815, 2112, 2229
FspBI CTAG 3 cut(s) 36, 489, 2393
GlaI GCGC 2 cut(s) 1704, 1800
GluI GCNGC 8 cut(s) 65, 350, 353, 356, 1568, 1815, 2112, 2229
GsaI CCCAGC 1 cut(s) 130
GsuI CTGGAG 4 cut(s) 1592, 1823, 2093, 2149
HaeII RGCGCY 1 cut(s) 1802
HaeIII GGCC 7 cut(s) 80, 366, 584, 631, 1004, 1452, 1734
HapII CCGG 3 cut(s) 75, 1033, 1735
HgaI GACGC 2 cut(s) 765, 1216
HhaI GCGC 2 cut(s) 1705, 1801
Hin1I GRCGYC 3 cut(s) 757, 1053, 1227
Hin6I GCGC 2 cut(s) 1703, 1799
HinP1I GCGC 2 cut(s) 1703, 1799
HindIII AAGCTT 2 cut(s) 566, 2207
HpaII CCGG 3 cut(s) 75, 1033, 1735
HphI GGTGA 5 cut(s) 316, 355, 1613, 1913, 2378
Hpy166II GTNNAC 5 cut(s) 145, 403, 853, 1835, 2341
Hpy8I GTNNAC 5 cut(s) 145, 403, 853, 1835, 2341
Hpy99I CGWCG 3 cut(s) 236, 437, 1055
HpyAV CCTTC 3 cut(s) 439, 1719, 1858
HpyCH4III ACNGT 5 cut(s) 304, 485, 1403, 1515, 1991
HpyCH4IV ACGT 4 cut(s) 432, 534, 638, 1053
HpyF10VI GCNNNNNNNGC 5 cut(s) 61, 355, 642, 938, 2108
HpyF3I CTNAG 8 cut(s) 56, 564, 801, 1070, 1699, 2115, 2148, 2260
HpySE526I ACGT 4 cut(s) 432, 534, 638, 1053
Hsp92I GRCGYC 3 cut(s) 757, 1053, 1227
HspAI GCGC 2 cut(s) 1703, 1799
KflI GGGWCCC 1 cut(s) 1372
KpnI GGTACC 1 cut(s) 1893
Ksp22I TGATCA 2 cut(s) 826, 1792
Kzo9I GATC 7 cut(s) 826, 859, 1792, 1939, 2233, 2373, 2424
LmnI GCTCC 6 cut(s) 15, 268, 381, 727, 1804, 2176
Lsp1109I GCAGC 7 cut(s) 361, 364, 367, 1579, 1826, 2098, 2240
LweI GCATC 1 cut(s) 2127
MaeI CTAG 3 cut(s) 36, 489, 2393
MaeII ACGT 4 cut(s) 432, 534, 638, 1053
MaeIII GTNAC 4 cut(s) 392, 653, 1779, 2366
MalI GATC 7 cut(s) 828, 861, 1794, 1941, 2235, 2375, 2426
MboI GATC 7 cut(s) 826, 859, 1792, 1939, 2233, 2373, 2424
MfeI CAATTG 1 cut(s) 1130
MflI RGATCY 3 cut(s) 859, 2233, 2424
MhlI GDGCHC 2 cut(s) 135, 290
MlsI TGGCCA 1 cut(s) 1004
MluNI TGGCCA 1 cut(s) 1004
MlyI GAGTC 7 cut(s) 96, 105, 151, 515, 738, 781, 1144
MmeI TCCRAC 4 cut(s) 79, 1042, 1876, 1903
Mox20I TGGCCA 1 cut(s) 1004
Mph1103I ATGCAT 2 cut(s) 1759, 2142
MroXI GAANNNNTTC 1 cut(s) 2388
MscI TGGCCA 1 cut(s) 1004
MseI TTAA 8 cut(s) 474, 893, 1119, 1428, 1479, 2030, 2036, 2201
MslI CAYNNNNRTG 2 cut(s) 338, 1017
Msp20I TGGCCA 1 cut(s) 1004
MspA1I CMGCKG 2 cut(s) 1570, 2413
MspI CCGG 3 cut(s) 75, 1033, 1735
MspR9I CCNGG 3 cut(s) 75, 76, 1736
MunI CAATTG 1 cut(s) 1130
Mva1269I GAATGC 2 cut(s) 1663, 1832
MwoI GCNNNNNNNGC 5 cut(s) 61, 355, 642, 938, 2108
NciI CCSGG 3 cut(s) 75, 76, 1736
NcoI CCATGG 3 cut(s) 1581, 2082, 2187
NdeII GATC 7 cut(s) 826, 859, 1792, 1939, 2233, 2373, 2424
NmeAIII GCCGAG 2 cut(s) 20, 2128
NmuCI GTSAC 3 cut(s) 653, 1779, 2366
NsiI ATGCAT 2 cut(s) 1759, 2142
NspI RCATGY 1 cut(s) 2453
PaeI GCATGC 1 cut(s) 2453
PaeR7I CTCGAG 1 cut(s) 368
PagI TCATGA 1 cut(s) 2095
PceI AGGCCT 2 cut(s) 584, 631
PcsI WCGNNNNNNNCGW 2 cut(s) 540, 549
PctI GAATGC 2 cut(s) 1663, 1832
PdmI GAANNNNTTC 1 cut(s) 2388
PfeI GAWTC 9 cut(s) 218, 259, 311, 462, 1612, 1875, 2077, 2194, 2321
PflFI GACNNNGTC 1 cut(s) 431
PflMI CCANNNNNTGG 1 cut(s) 666
PinAI ACCGGT 1 cut(s) 1032
PkrI GCNGC 8 cut(s) 66, 351, 354, 357, 1569, 1816, 2113, 2230
PleI GAGTC 7 cut(s) 96, 104, 151, 515, 738, 781, 1144
PpsI GAGTC 7 cut(s) 96, 104, 151, 515, 738, 781, 1144
PpuMI RGGWCCY 1 cut(s) 1372
PsiI TTATAA 1 cut(s) 606
Psp5II RGGWCCY 1 cut(s) 1372
PspEI GGTNACC 1 cut(s) 2366
PspFI CCCAGC 1 cut(s) 126
PspPI GGNCC 6 cut(s) 78, 364, 403, 886, 1372, 2013
PspPPI RGGWCCY 1 cut(s) 1372
PsrI GAACNNNNNNTAC 2 cut(s) 1986, 2018
PstI CTGCAG 1 cut(s) 964
PstNI CAGNNNCTG 1 cut(s) 666
PsuI RGATCY 3 cut(s) 859, 2233, 2424
PsyI GACNNNGTC 1 cut(s) 431
PvuII CAGCTG 2 cut(s) 1570, 2413
RsaI GTAC 4 cut(s) 332, 487, 1712, 1891
RsaNI GTAC 4 cut(s) 331, 486, 1711, 1890
RseI CAYNNNNRTG 2 cut(s) 338, 1017
SaqAI TTAA 8 cut(s) 474, 893, 1119, 1428, 1479, 2030, 2036, 2201
SatI GCNGC 8 cut(s) 65, 350, 353, 356, 1568, 1815, 2112, 2229
Sau3AI GATC 7 cut(s) 826, 859, 1792, 1939, 2233, 2373, 2424
Sau96I GGNCC 6 cut(s) 78, 364, 403, 886, 1372, 2013
ScaI AGTACT 1 cut(s) 487
SchI GAGTC 7 cut(s) 96, 105, 151, 515, 738, 781, 1144
ScrFI CCNGG 3 cut(s) 75, 76, 1736
SduI GDGCHC 2 cut(s) 135, 290
SfaNI GCATC 1 cut(s) 2127
SfcI CTRYAG 1 cut(s) 960
Sfr274I CTCGAG 1 cut(s) 368
SinI GGWCC 4 cut(s) 403, 886, 1372, 2013
SlaI CTCGAG 1 cut(s) 368
SmaI CCCGGG 1 cut(s) 76
SmiI ATTTAAAT 2 cut(s) 2031, 2037
SmiMI CAYNNNNRTG 2 cut(s) 338, 1017
SmlI CTYRAG 2 cut(s) 368, 1820
SmoI CTYRAG 2 cut(s) 368, 1820
SpeI ACTAGT 1 cut(s) 488
SphI GCATGC 1 cut(s) 2453
SseBI AGGCCT 2 cut(s) 584, 631
SspI AATATT 1 cut(s) 1559
SspMI CTAG 3 cut(s) 36, 489, 2393
StuI AGGCCT 2 cut(s) 584, 631
StyD4I CCNGG 3 cut(s) 73, 74, 1734
StyI CCWWGG 4 cut(s) 1581, 1974, 2082, 2187
SwaI ATTTAAAT 2 cut(s) 2031, 2037
TaaI ACNGT 5 cut(s) 304, 485, 1403, 1515, 1991
TaiI ACGT 4 cut(s) 435, 537, 641, 1056
TaqI TCGA 4 cut(s) 49, 369, 460, 543
TatI WGTACW 2 cut(s) 330, 485
TauI GCSGC 1 cut(s) 67
TfiI GAWTC 9 cut(s) 218, 259, 311, 462, 1612, 1875, 2077, 2194, 2321
Tru1I TTAA 8 cut(s) 474, 893, 1119, 1428, 1479, 2030, 2036, 2201
Tru9I TTAA 8 cut(s) 474, 893, 1119, 1428, 1479, 2030, 2036, 2201
TscAI CASTG 3 cut(s) 194, 667, 1788
TseFI GTSAC 3 cut(s) 653, 1779, 2366
TseI GCWGC 7 cut(s) 349, 352, 355, 1567, 1814, 2111, 2228
Tsp45I GTSAC 3 cut(s) 653, 1779, 2366
TspDTI ATGAA 8 cut(s) 529, 832, 1224, 1998, 2084, 2112, 2294, 2306
TspGWI ACGGA 3 cut(s) 194, 1322, 2005
TspMI CCCGGG 1 cut(s) 74
TspRI CASTG 3 cut(s) 194, 667, 1788
Tth111I GACNNNGTC 1 cut(s) 431
Van91I CCANNNNNTGG 1 cut(s) 666
VpaK11BI GGWCC 4 cut(s) 403, 886, 1372, 2013
XagI CCTNNNNNAGG 1 cut(s) 1973
XapI RAATTY 3 cut(s) 1588, 2032, 2152
XbaI TCTAGA 1 cut(s) 2392
XceI RCATGY 1 cut(s) 2453
XcmI CCANNNNNNNNNTGG 2 cut(s) 706, 997
XhoI CTCGAG 1 cut(s) 368
XmaI CCCGGG 1 cut(s) 74
XmiI GTMKAC 2 cut(s) 144, 2340
XmnI GAANNNNTTC 1 cut(s) 2388
XspI CTAG 3 cut(s) 36, 489, 2393
ZraI GACGTC 1 cut(s) 1054
ZrmI AGTACT 1 cut(s) 487
Zsp2I ATGCAT 2 cut(s) 1759, 2142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.