Rorug02G0597500

CarD-like/TRCF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0015231
Physical Location & Seq
Reverse (-)
71820576 .. 71822043
1468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0597500.1

Sequence Viewer

Length: 1188 bp
ATGGCTTCGTCCTCTACTTATGACGTGTTTTTGAGCTTCAGGGGCATAGACACTTACGAAGGCTTCACCAGCCACCTATATGATGCTTTGAAGCGTGCTGATAGAGATCTTGAAGTTGGAACAACCATTAGCCCCAAACTGCTTAAAGCAATCCAAAGGTCTAGGATTTCTGTCATCGTTATGTCAGGGAGCTATGCATTTTCGGGATGGTGTCTTAATGAACTGCTTTGCATCATGGAATCATGGAGAAGGGAAGATAGATTGGTTTTGCCAATATTTTATAATGTCGATCCATCTGACGTCAGGAAACAGAAGGGTGGTTTTGGTGAGGCACTAGAGGAGCATCATCGTTCCGGTCGGATAGAGCAAACAACAATGCTCAAGTGGAGGGAGGCTCTCACTCAAGTTGCCAATATATGCGGCTGGGATACCAAAAAAGAGAGGCACTCAGAGTTAGTCTATGACATTGTAAAGTCTATCCAAAGTAAAGGACTTCCGACAAGGCTGAACATTTCCATCTATCCCCTCAGATATGATACGTGTGATGAATATCTCAAAGGTTTTTTGCAATTAGGATCACAGGAAGTTGTTATAGTAGGGATCTGCGGACCAGGCCAAATGGGAAAGAGAGCTATGGCCAGAGTTATGTATGACCAAATTCTACAAAATTTTGATGGTGGCTGCTTCCTCGCAGATGTTGGCCAAAGATCAAATCAACCCAACTATCTGGTTCATCTACAAGAAACACTTCTTTCTGAAATTCTCTCAGAGAGGAATGTAAAGATCTCAGATACTAGAATGGGAATAGATCTGATTGCACAAAATTTGTGCTCCAAAAGGGTTTTAGTTGTTTTGGACAATGTGGATCATTTGAGTCAAATCTATGCACTGGTTGGAGATTGCAAGTTTTTCGGTCCGGGAAGTAAAATCATCATAACGACTAGAGATGTGCAACTGCTAAACCTGTTTGGAGTGGACCAGTTCATTTTGGCTCATGGCCGTCCTCTGCCAGAAAATGTTGGGGCCATCTGGGAGCCATATCAGCGAAGTCGTTGCATAGTTGCAGGAATCAAGGAGTACATTGAGACCTTTAATCAACAGAGACGAGAGAGGTTGAAGCACCTTCAAGCTGTTTTTCTCCAAGAGCGCCAGAAAAACGCAACTCAGGTACTCTTTCTTATGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarD_CdnL_TRCF DEAD Helicase_C TRCF

Protein Analysis

395

Amino Acids

45.12

Weight (kDa)

8.29

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 7 - 166 5.2e-42 TIR domain
TIR_2 PF13676 9 - 98 2.1e-07 TIR domain
NB-ARC PF00931 188 - 322 2.5e-09 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02060 AT3G02060 AT3G02060
fragaria_vesca FvH4_6g54430
malus_domestica MD17G1004500.v1.1
prunus_persica Prupe.3G314900_v2.0.a1
pyrus_communis pycom111g00110 pycom17g00110
rosa_chinensis RchiOBHm_Chr2g0176681
rosa_laevigata RLG00000022435
rosa_rugosa Rorug02G0597500
rosa_samantha Rh2BG686000 Rh2CG649300 Rh2DG700200
rosa_wichuraiana Rw0G015680 Rw2G055390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 282
AatII GACGTC 1 cut(s) 303
AciI CCGC 2 cut(s) 420, 606
AclWI GGATC 4 cut(s) 284, 583, 608, 873
AcoI YGGCCR 3 cut(s) 636, 700, 997
AcsI RAATTY 4 cut(s) 657, 667, 759, 823
AcuI CTGAAG 1 cut(s) 22
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 2 cut(s) 1079, 1170
AflIII ACRYGT 2 cut(s) 24, 539
AgsI TTSAA 4 cut(s) 91, 113, 1117, 1127
AjiI CACGTC 1 cut(s) 25
AjnI CCWGG 1 cut(s) 610
AluBI AGCT 4 cut(s) 36, 192, 632, 1130
AluI AGCT 4 cut(s) 36, 192, 632, 1130
Alw21I GWGCWC 1 cut(s) 833
Alw26I GTCTC 2 cut(s) 1079, 1096
AlwI GGATC 4 cut(s) 284, 583, 608, 873
AoxI GGCC 5 cut(s) 613, 636, 700, 997, 1023
ApeKI GCWGC 1 cut(s) 681
ApoI RAATTY 4 cut(s) 657, 667, 759, 823
Asp700I GAANNNNTTC 1 cut(s) 747
AspLEI GCGC 1 cut(s) 1149
AspS9I GGNCC 4 cut(s) 608, 914, 976, 1023
AsuC2I CCSGG 1 cut(s) 918
AsuHPI GGTGA 2 cut(s) 58, 338
AvaII GGWCC 3 cut(s) 608, 914, 976
BalI TGGCCA 2 cut(s) 638, 702
Bbv12I GWGCWC 1 cut(s) 833
BbvI GCAGC 1 cut(s) 668
BccI CCATC 5 cut(s) 201, 301, 524, 668, 1034
BceAI ACGGC 1 cut(s) 984
BcgI CGANNNNNNTGC 4 cut(s) 892, 926, 1035, 1069
BciT130I CCWGG 1 cut(s) 612
BciVI GTATCC 1 cut(s) 421
BcnI CCSGG 1 cut(s) 918
BcoDI GTCTC 2 cut(s) 1079, 1096
BfaI CTAG 4 cut(s) 162, 335, 795, 942
BfoI RGCGCY 1 cut(s) 1150
BfuI GTATCC 1 cut(s) 421
BglII AGATCT 3 cut(s) 106, 783, 808
BisI GCNGC 2 cut(s) 421, 682
BlsI GCNGC 2 cut(s) 422, 683
Bme1390I CCNGG 2 cut(s) 612, 918
Bme18I GGWCC 3 cut(s) 608, 914, 976
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 4 cut(s) 608, 914, 976, 1023
BmiI GGNNCC 2 cut(s) 1024, 1035
BmrFI CCNGG 2 cut(s) 612, 918
BmsI GCATC 3 cut(s) 73, 240, 352
BplI GAGNNNNNCTC 4 cut(s) 379, 411, 431, 463
BpuEI CTTGAG 2 cut(s) 365, 387
BpuMI CCSGG 1 cut(s) 918
BsaAI YACGTR 1 cut(s) 540
BsaBI GATNNNNATC 2 cut(s) 105, 549
BsaHI GRCGYC 1 cut(s) 300
BsaI GGTCTC 1 cut(s) 1079
BsaWI WCCGGW 1 cut(s) 353
Bse1I ACTGG 2 cut(s) 894, 979
Bse8I GATNNNNATC 2 cut(s) 105, 549
BseBI CCWGG 1 cut(s) 612
BseGI GGATG 1 cut(s) 212
BseJI GATNNNNATC 2 cut(s) 105, 549
BseMII CTCAG 5 cut(s) 462, 541, 780, 801, 1178
BseNI ACTGG 2 cut(s) 894, 979
BseRI GAGGAG 1 cut(s) 353
BseXI GCAGC 1 cut(s) 668
BseYI CCCAGC 1 cut(s) 423
Bsh1285I CGRYCG 1 cut(s) 358
BshFI GGCC 5 cut(s) 615, 638, 702, 999, 1025
BsiEI CGRYCG 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 833
BsiSI CCGG 2 cut(s) 354, 917
BsmAI GTCTC 2 cut(s) 1079, 1096
BsmBI CGTCTC 1 cut(s) 1096
BsnI GGCC 5 cut(s) 615, 638, 702, 999, 1025
Bso31I GGTCTC 1 cut(s) 1079
Bsp1286I GDGCHC 1 cut(s) 833
Bsp143I GATC 8 cut(s) 106, 289, 575, 600, 707, 783, 808, 865
BspACI CCGC 2 cut(s) 420, 606
BspANI GGCC 5 cut(s) 615, 638, 702, 999, 1025
BspCNI CTCAG 5 cut(s) 461, 540, 779, 800, 1177
BspLI GGNNCC 2 cut(s) 1024, 1035
BspPI GGATC 4 cut(s) 284, 583, 608, 873
BspTNI GGTCTC 1 cut(s) 1079
BsrI ACTGG 2 cut(s) 894, 979
BssMI GATC 8 cut(s) 106, 289, 575, 600, 707, 783, 808, 865
BssNI GRCGYC 1 cut(s) 300
Bst2UI CCWGG 1 cut(s) 612
BstACI GRCGYC 1 cut(s) 300
BstBAI YACGTR 1 cut(s) 540
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 5 cut(s) 448, 527, 766, 787, 1164
BstF5I GGATG 1 cut(s) 212
BstH2I RGCGCY 1 cut(s) 1150
BstHHI GCGC 1 cut(s) 1149
BstKTI GATC 8 cut(s) 109, 292, 578, 603, 710, 786, 811, 868
BstMAI GTCTC 2 cut(s) 1079, 1096
BstMBI GATC 8 cut(s) 106, 289, 575, 600, 707, 783, 808, 865
BstMCI CGRYCG 1 cut(s) 358
BstMWI GCNNNNNNNGC 4 cut(s) 42, 69, 612, 1042
BstNI CCWGG 1 cut(s) 612
BstSCI CCNGG 2 cut(s) 610, 916
BstV1I GCAGC 1 cut(s) 668
BstX2I RGATCY 4 cut(s) 106, 600, 783, 808
BstXI CCANNNNNNTGG 1 cut(s) 727
BstYI RGATCY 4 cut(s) 106, 600, 783, 808
BsuI GTATCC 1 cut(s) 421
BsuRI GGCC 5 cut(s) 615, 638, 702, 999, 1025
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 1 cut(s) 212
BtsIMutI CAGTG 1 cut(s) 887
Cac8I GCNNGC 1 cut(s) 96
CfoI GCGC 1 cut(s) 1149
Cfr13I GGNCC 4 cut(s) 608, 914, 976, 1023
CpoI CGGWCCG 1 cut(s) 914
Csp6I GTAC 2 cut(s) 1078, 1169
CspI CGGWCCG 1 cut(s) 914
CviAII CATG 3 cut(s) 235, 243, 995
CviQI GTAC 2 cut(s) 1078, 1169
DdeI CTNAG 5 cut(s) 448, 527, 766, 787, 1164
DpnI GATC 8 cut(s) 108, 291, 577, 602, 709, 785, 810, 867
DpnII GATC 8 cut(s) 106, 289, 575, 600, 707, 783, 808, 865
EaeI YGGCCR 3 cut(s) 636, 700, 997
Eco31I GGTCTC 1 cut(s) 1079
Eco47I GGWCC 3 cut(s) 608, 914, 976
Eco57I CTGAAG 1 cut(s) 22
EcoRII CCWGG 1 cut(s) 610
EcoT22I ATGCAT 1 cut(s) 199
Esp3I CGTCTC 1 cut(s) 1096
FaeI CATG 3 cut(s) 238, 246, 998
FalI AAGNNNNNCTT 2 cut(s) 732, 764
FatI CATG 3 cut(s) 234, 242, 994
Fnu4HI GCNGC 2 cut(s) 421, 682
FokI GGATG 1 cut(s) 219
Fsp4HI GCNGC 2 cut(s) 421, 682
FspBI CTAG 4 cut(s) 162, 335, 795, 942
GlaI GCGC 1 cut(s) 1148
GluI GCNGC 2 cut(s) 421, 682
GsaI CCCAGC 1 cut(s) 427
HaeII RGCGCY 1 cut(s) 1150
HaeIII GGCC 5 cut(s) 615, 638, 702, 999, 1025
HapII CCGG 2 cut(s) 354, 917
HhaI GCGC 1 cut(s) 1149
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 3 cut(s) 238, 246, 998
Hin6I GCGC 1 cut(s) 1147
HinP1I GCGC 1 cut(s) 1147
HinfI GANTC 3 cut(s) 239, 874, 1068
HpaII CCGG 2 cut(s) 354, 917
HphI GGTGA 2 cut(s) 58, 338
Hpy166II GTNNAC 1 cut(s) 976
Hpy188I TCNGA 9 cut(s) 298, 360, 451, 498, 530, 757, 769, 790, 813
Hpy188III TCNNGA 3 cut(s) 110, 204, 304
Hpy8I GTNNAC 1 cut(s) 976
HpyAV CCTTC 4 cut(s) 53, 243, 307, 1133
HpyCH4IV ACGT 3 cut(s) 24, 300, 539
HpyCH4V TGCA 9 cut(s) 197, 231, 568, 818, 887, 903, 952, 1056, 1064
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 69, 612, 1042
HpyF3I CTNAG 5 cut(s) 448, 527, 766, 787, 1164
HpySE526I ACGT 3 cut(s) 24, 300, 539
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 3 cut(s) 238, 246, 998
HspAI GCGC 1 cut(s) 1147
Kzo9I GATC 8 cut(s) 106, 289, 575, 600, 707, 783, 808, 865
LmnI GCTCC 4 cut(s) 189, 340, 836, 1033
Lsp1109I GCAGC 1 cut(s) 668
LweI GCATC 3 cut(s) 73, 240, 352
MaeI CTAG 4 cut(s) 162, 335, 795, 942
MaeII ACGT 3 cut(s) 24, 300, 539
MalI GATC 8 cut(s) 108, 291, 577, 602, 709, 785, 810, 867
MboI GATC 8 cut(s) 106, 289, 575, 600, 707, 783, 808, 865
MboII GAAGA 1 cut(s) 266
MflI RGATCY 4 cut(s) 106, 600, 783, 808
MhlI GDGCHC 1 cut(s) 833
MlsI TGGCCA 2 cut(s) 638, 702
MluCI AATT 5 cut(s) 569, 657, 667, 759, 823
MluNI TGGCCA 2 cut(s) 638, 702
MlyI GAGTC 1 cut(s) 883
MmeI TCCRAC 4 cut(s) 97, 338, 521, 874
Mox20I TGGCCA 2 cut(s) 638, 702
Mph1103I ATGCAT 1 cut(s) 199
MroXI GAANNNNTTC 1 cut(s) 747
MscI TGGCCA 2 cut(s) 638, 702
MseI TTAA 3 cut(s) 144, 216, 1092
MslI CAYNNNNRTG 2 cut(s) 78, 179
Msp20I TGGCCA 2 cut(s) 638, 702
MspI CCGG 2 cut(s) 354, 917
MspR9I CCNGG 2 cut(s) 612, 918
MvaI CCWGG 1 cut(s) 612
MwoI GCNNNNNNNGC 4 cut(s) 42, 69, 612, 1042
NciI CCSGG 1 cut(s) 918
NdeII GATC 8 cut(s) 106, 289, 575, 600, 707, 783, 808, 865
NlaIII CATG 3 cut(s) 238, 246, 998
NlaIV GGNNCC 2 cut(s) 1024, 1035
NsiI ATGCAT 1 cut(s) 199
PdmI GAANNNNTTC 1 cut(s) 747
PfeI GAWTC 2 cut(s) 239, 1068
PfoI TCCNGGA 1 cut(s) 916
PkrI GCNGC 2 cut(s) 422, 683
PleI GAGTC 1 cut(s) 882
PpsI GAGTC 1 cut(s) 882
Ppu21I YACGTR 1 cut(s) 540
PsiI TTATAA 1 cut(s) 282
Psp6I CCWGG 1 cut(s) 610
PspFI CCCAGC 1 cut(s) 423
PspGI CCWGG 1 cut(s) 610
PspN4I GGNNCC 2 cut(s) 1024, 1035
PspPI GGNCC 4 cut(s) 608, 914, 976, 1023
PsuI RGATCY 4 cut(s) 106, 600, 783, 808
RsaI GTAC 2 cut(s) 1079, 1170
RsaNI GTAC 2 cut(s) 1078, 1169
RseI CAYNNNNRTG 2 cut(s) 78, 179
Rsr2I CGGWCCG 1 cut(s) 914
RsrII CGGWCCG 1 cut(s) 914
SaqAI TTAA 3 cut(s) 144, 216, 1092
SatI GCNGC 2 cut(s) 421, 682
Sau3AI GATC 8 cut(s) 106, 289, 575, 600, 707, 783, 808, 865
Sau96I GGNCC 4 cut(s) 608, 914, 976, 1023
SchI GAGTC 1 cut(s) 883
ScrFI CCNGG 2 cut(s) 612, 918
SduI GDGCHC 1 cut(s) 833
SfaNI GCATC 3 cut(s) 73, 240, 352
SinI GGWCC 3 cut(s) 608, 914, 976
SmiMI CAYNNNNRTG 2 cut(s) 78, 179
SmlI CTYRAG 2 cut(s) 380, 402
SmoI CTYRAG 2 cut(s) 380, 402
Sse9I AATT 5 cut(s) 569, 657, 667, 759, 823
SsiI CCGC 2 cut(s) 420, 606
SspI AATATT 1 cut(s) 276
SspMI CTAG 4 cut(s) 162, 335, 795, 942
StyD4I CCNGG 2 cut(s) 610, 916
TaiI ACGT 3 cut(s) 27, 303, 542
TaqI TCGA 1 cut(s) 288
TaqII GACCGA 1 cut(s) 902
TasI AATT 5 cut(s) 569, 657, 667, 759, 823
TatI WGTACW 1 cut(s) 1077
TauI GCSGC 1 cut(s) 423
TfiI GAWTC 2 cut(s) 239, 1068
Tru1I TTAA 3 cut(s) 144, 216, 1092
Tru9I TTAA 3 cut(s) 144, 216, 1092
TscAI CASTG 1 cut(s) 894
TseI GCWGC 1 cut(s) 681
TspDTI ATGAA 4 cut(s) 234, 561, 722, 973
TspRI CASTG 1 cut(s) 894
VpaK11BI GGWCC 3 cut(s) 608, 914, 976
XapI RAATTY 4 cut(s) 657, 667, 759, 823
XmnI GAANNNNTTC 1 cut(s) 747
XspI CTAG 4 cut(s) 162, 335, 795, 942
ZraI GACGTC 1 cut(s) 301
Zsp2I ATGCAT 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.