Rw2G055390

CarD-like/TRCF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0015231
Physical Location & Seq
Forward (+)
82777757 .. 82783363
5607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G055390.1

Sequence Viewer

Length: 2478 bp
ATGGCTCTGCTCCTCCCCATCCCAATCCCAATCCCAATCCATGGCGTTTCTTCTTCTTCTTCCTTTTGTTATAGATCGAGGTCGGCGAGTCGGCTGTGCAGGGTTTGGGGTTGGGGACTGTTGAAAATCCAGAGAGGGGGCCGCACCAAGACGCCGACGCCGACGAAGAACAGCCTGGGCCTTTCCAATTCCACTTTCACGAAAACCAAATCGAGCAAAAAGCGACGGGAGGCGGCTGTGGAGGCAGAGTCGGACGCCATATCGATTCTAAACGAGAGAATTCGGCGCGAGCAGAGAAAGAAATCGTCGGAGGGGTCTCCGATGGAGGCGGAGGAGGCAGACAAGTACTTGGAGCTGGTGAAGCAGCAGCAGCAGAGGGGCCTCCAGAAGCTCAAAGGAGACCTTAGTTACAAGGTCGACCCCTACACTCTCCGTTCCGGTGATTATGTGGTCCATAAGAAGGTCGGCCTCGGTCGATTCGTTGGGATTAAATTCGACAATGCTCCTGCTTCCATGGACCCTATTGAATACGTCTTCATTGAGTATGCTGATGGCATGGCTAAGCTTCCTGTTAAGCAGGCCTCTCGCTTGCTCTATCGTTACAATCTCCCCAACGAAAACAAAAAACCTCACACATTGAGCAAACTGAACGACACCAGTGTGTGGGAGAGAAGAAAGACCAAGGGAAAGATTGCCATTCAGAAAATGGTTGTTGATCTGATGGAGTTGTATTTACACAGGCTCAAACAAACACGCCCTCCCTACCCTCTTACTCCTGCCATGGCTCACTTTGTTTCTCAGTTTCCGTATGAGCCTACTCCTGATCAAAAGCAGGCTTTCATTGATGTTGAGAAAGATTTAACAGGGAGGGAAACTCCAATGGACCGCTTAATTTGTGGAGACGTTGGCTTTGGCAAAACTGAAGTTGCACTCCGTGCCATCTTTTGTGTAGTTTCCGCAGGGAAGCAAGCCATGGTTCTTGCACCCACAATTGTTTTAGCCAAGCAACATTTTGAAGTTATCTCACAGCGATTTTCTATCTACCCAAATATCAACGTTGGACTCCTTAGCAGGTTTCAGACTAAATCAGAGAAGGATGAGCATCTAGACATGATAAAGAATGGTCATTTGGACATAATTGTTGGGACACACTCACTTCTTGGAAGTCGTGTTGTGTATAATAATCTAGGCCTGCTTGTCGTTGATGAAGAACAGAGGTTTGGCGTCAAACAGAAAGAGAAGATTGCTTCATTCAAAACATCAGTTGATGTTCTTACTCTATCTGCAACACCTATACCGCGAACCCTTTATTTAGCATTGACTGGTTTTCGTGATGCTAGCTTGATTTCAACTCCACCTCCAGAAAGAGTCCCTATAAGAACCCATCTCTCGGCATACAGTAAAGAAAAGGTATTATCAGCAATTAAGTATGAGCTGGAGCGAGGTGGCCAAGTATTTTATGTTTTGCCTCGTATCAAAGGACTTGAAGAAGTGATGAGTTTTCTTGAACAGTCGTTTCCTGATGTTGAAATAGCTATTGCACACGGGAAGCAATATTCAAAGCAGCTAGAGGAAACCATGGAGAAATTTTCACAAGGTGAAATCAAGATTCTTACATGCACTAATATAGTAGAAAGTGGGCTTGACATTCAGAATGCAAACACCATTATAATTCAGGATGTTCAACAATTTGGCCTAGCACAGTTGTATCAGCTGCGAGGAAGGGTGGGGAGGGCAGATAAGGAAGCTCACGCACACTTATTTTATCCAGAGAAGTCACTTCTAACTGATCAGGCATTGGAGAGACTTGCAGCTCTAGAGGAATGCTGTCAACTTGGGCAAGGTTTCCAACTTGCAGAGAGGGACATGGGAATAAGAGGCTTTGGTACCATCTTTGGTGAGCAGCAGACTGGGGATGTTGGAAATGTTGGCATTGATCTCTTCTTTGAAATGCTGTTTGAGAGCTTGTCTAAGGTTGATGAGCATCGAGTAGTTTCAGTTCCTTACTGGTCCGTTGAGATTGATATGAATATAAATCCTCATCTCCCTTCTGAGTACATAAATAATCTCGAGAATCCCATGGAGATAATTCATGAAGCTGAGAAAGCTGCAGAGAAGGATATCTGGAGTTTGATGCAATATACTGAGAATTTGCGGCGTCAATATGGAAAGGAGCCACACTCCATGGAAATTTTATTAAAGAAGCTTTATGTGAGGAGAATGGCAGCAGACATTGGAATAACCAAGATATATGCTTCTGGAAAGATGGTTTTTATGAAAACAGTTATGAATAAACATGTTTTCAATCTGATAACAGATTCAGTGGTTTCTGATGTCCACCGAAATTCTTTGGTATTTGAGGGTGACCAGATCAAGGCAGAACTTCTTCTAGAATTGCCAAGAGAACAACTCCTAAACTGGATATTTCGATGTTTAGCTGAACTACATGCTTCCTTACCTTCCCTCATAAAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarD_CdnL_TRCF DEAD Helicase_C TRCF

Protein Analysis

825

Amino Acids

93.56

Weight (kDa)

8.84

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CarD_TRCF_RID PF02559 144 - 201 2.2e-09 CarD-like/TRCF RID domain
ResIII PF04851 271 - 431 3.3e-09 Type III restriction enzyme, res subunit
DEAD PF00270 273 - 435 1.7e-23 DEAD/DEAH box helicase
Helicase_C PF00271 473 - 579 1.9e-20 Helicase conserved C-terminal domain
TRCF PF03461 677 - 763 1.3e-06 TRCF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02060 AT3G02060 AT3G02060
fragaria_vesca FvH4_6g54430
malus_domestica MD17G1004500.v1.1
prunus_persica Prupe.3G314900_v2.0.a1
pyrus_communis pycom111g00110 pycom17g00110
rosa_chinensis RchiOBHm_Chr2g0176681
rosa_laevigata RLG00000022435
rosa_rugosa Rorug02G0597500
rosa_samantha Rh2BG686000 Rh2CG649300 Rh2DG700200
rosa_wichuraiana Rw0G015680 Rw2G055390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1670
Acc36I ACCTGC 1 cut(s) 1062
Acc65I GGTACC 1 cut(s) 1886
AccB1I GGYRCC 1 cut(s) 1886
AccB7I CCANNNNNTGG 2 cut(s) 41, 663
AccI GTMKAC 1 cut(s) 417
AccII CGCG 2 cut(s) 288, 1300
AciI CCGC 7 cut(s) 142, 233, 329, 886, 957, 1298, 2155
AclI AACGTT 1 cut(s) 1056
AcoI YGGCCR 1 cut(s) 1447
AcsI RAATTY 6 cut(s) 279, 491, 1586, 2149, 2190, 2344
AcuI CTGAAG 1 cut(s) 942
AcyI GRCGYC 5 cut(s) 152, 158, 255, 1224, 2158
AdeI CACNNNGTG 2 cut(s) 935, 1598
AfaI GTAC 4 cut(s) 347, 1888, 2057, 2474
AfiI CCNNNNNNNGG 6 cut(s) 41, 136, 460, 663, 1390, 2374
AflIII ACRYGT 1 cut(s) 2296
AjnI CCWGG 1 cut(s) 174
AjuI GAANNNNNNNTTGG 6 cut(s) 179, 211, 1112, 1144, 1203, 1235
AleI CACNNNNGTG 1 cut(s) 659
Alw26I GTCTC 4 cut(s) 321, 393, 894, 1798
Ama87I CYCGRG 1 cut(s) 2069
AoxI GGCC 8 cut(s) 139, 178, 379, 466, 579, 1189, 1447, 1693
ApeKI GCWGC 9 cut(s) 364, 367, 370, 1564, 1714, 1811, 1903, 2108, 2225
ApoI RAATTY 6 cut(s) 279, 491, 1586, 2149, 2190, 2344
ArsI GACNNNNNNTTYG 2 cut(s) 893, 925
Asp700I GAANNNNTTC 1 cut(s) 2385
Asp718I GGTACC 1 cut(s) 1886
AspLEI GCGC 1 cut(s) 288
AspS9I GGNCC 7 cut(s) 139, 178, 379, 451, 517, 883, 2010
AsuHPI GGTGA 5 cut(s) 370, 452, 1610, 1910, 2375
AsuNHI GCTAGC 1 cut(s) 1337
AvaI CYCGRG 1 cut(s) 2069
AvaII GGWCC 4 cut(s) 451, 517, 883, 2010
BaeI ACNNNNGTAYC 2 cut(s) 1691, 1724
BalI TGGCCA 1 cut(s) 1449
BanI GGYRCC 1 cut(s) 1886
BbsI GAAGAC 1 cut(s) 526
BbvI GCAGC 9 cut(s) 376, 379, 382, 1576, 1701, 1823, 1915, 2095, 2237
BccI CCATC 8 cut(s) 26, 316, 545, 715, 947, 1392, 1898, 2260
BcgI CGANNNNNNTGC 2 cut(s) 566, 600
BciT130I CCWGG 1 cut(s) 176
BclI TGATCA 2 cut(s) 823, 1789
BcoDI GTCTC 4 cut(s) 321, 393, 894, 1798
BfaI CTAG 8 cut(s) 1106, 1187, 1338, 1568, 1697, 1817, 2390, 2476
BfmI CTRYAG 1 cut(s) 2109
BfuAI ACCTGC 1 cut(s) 1062
BlpI GCTNAGC 1 cut(s) 561
BmcAI AGTACT 2 cut(s) 347, 2474
Bme1390I CCNGG 1 cut(s) 176
Bme18I GGWCC 4 cut(s) 451, 517, 883, 2010
BmeT110I CYCGRG 1 cut(s) 2069
BmgT120I GGNCC 7 cut(s) 139, 178, 379, 451, 517, 883, 2010
BmiI GGNNCC 5 cut(s) 140, 380, 519, 1888, 2175
BmrFI CCNGG 1 cut(s) 176
BmrI ACTGGG 1 cut(s) 1920
BmsI GCATC 4 cut(s) 1111, 1324, 1993, 2124
BmtI GCTAGC 1 cut(s) 1341
BmuI ACTGGG 1 cut(s) 1920
BpiI GAAGAC 1 cut(s) 526
BplI GAGNNNNNCTC 6 cut(s) 859, 891, 2165, 2197, 2394, 2426
BpmI CTGGAG 4 cut(s) 368, 1344, 1457, 2146
Bpu10I CCTNAGC 1 cut(s) 1067
Bpu1102I GCTNAGC 1 cut(s) 561
Bsa29I ATCGAT 1 cut(s) 263
BsaBI GATNNNNATC 2 cut(s) 1101, 1983
BsaHI GRCGYC 5 cut(s) 152, 158, 255, 1224, 2158
BsaI GGTCTC 2 cut(s) 321, 393
BsaWI WCCGGW 1 cut(s) 437
BsaXI ACNNNNNCTCC 6 cut(s) 233, 263, 742, 772, 1342, 1372
Bsc4I CCNNNNNNNGG 6 cut(s) 41, 136, 460, 663, 1390, 2374
Bse1I ACTGG 5 cut(s) 657, 1327, 1915, 2012, 2423
Bse8I GATNNNNATC 2 cut(s) 1101, 1983
BseBI CCWGG 1 cut(s) 176
BseCI ATCGAT 1 cut(s) 263
BseGI GGATG 4 cut(s) 18, 1102, 1684, 1921
BseJI GATNNNNATC 2 cut(s) 1101, 1983
BseLI CCNNNNNNNGG 6 cut(s) 41, 136, 460, 663, 1390, 2374
BseMII CTCAG 4 cut(s) 812, 2043, 2091, 2136
BseNI ACTGG 5 cut(s) 657, 1327, 1915, 2012, 2423
BseRI GAGGAG 2 cut(s) 347, 2230
BseXI GCAGC 9 cut(s) 376, 379, 382, 1576, 1701, 1823, 1915, 2095, 2237
BsgI GTGCAG 1 cut(s) 118
Bsh1236I CGCG 2 cut(s) 288, 1300
Bsh1285I CGRYCG 1 cut(s) 475
BshFI GGCC 8 cut(s) 141, 180, 381, 468, 581, 1191, 1449, 1695
BshNI GGYRCC 1 cut(s) 1886
BshVI ATCGAT 1 cut(s) 263
BsiEI CGRYCG 1 cut(s) 475
BsiHKCI CYCGRG 1 cut(s) 2069
BsiSI CCGG 1 cut(s) 438
BslFI GGGAC 4 cut(s) 129, 1159, 1355, 1877
BslI CCNNNNNNNGG 6 cut(s) 41, 136, 460, 663, 1390, 2374
BsmAI GTCTC 4 cut(s) 321, 393, 894, 1798
BsmBI CGTCTC 1 cut(s) 894
BsmFI GGGAC 4 cut(s) 129, 1159, 1355, 1877
BsmI GAATGC 2 cut(s) 1660, 1829
BsnI GGCC 8 cut(s) 141, 180, 381, 468, 581, 1191, 1449, 1695
Bso31I GGTCTC 2 cut(s) 321, 393
BsoBI CYCGRG 1 cut(s) 2069
Bsp143I GATC 6 cut(s) 74, 715, 823, 1789, 1936, 2370
Bsp1720I GCTNAGC 1 cut(s) 561
Bsp19I CCATGG 7 cut(s) 40, 513, 780, 972, 1578, 2079, 2184
BspACI CCGC 7 cut(s) 142, 233, 329, 886, 957, 1298, 2155
BspANI GGCC 8 cut(s) 141, 180, 381, 468, 581, 1191, 1449, 1695
BspCNI CTCAG 4 cut(s) 811, 2044, 2092, 2137
BspDI ATCGAT 1 cut(s) 263
BspFNI CGCG 2 cut(s) 288, 1300
BspHI TCATGA 1 cut(s) 2092
BspLI GGNNCC 5 cut(s) 140, 380, 519, 1888, 2175
BspMAI CTGCAG 1 cut(s) 2113
BspMI ACCTGC 1 cut(s) 1062
BspOI GCTAGC 1 cut(s) 1341
BspT107I GGYRCC 1 cut(s) 1886
BspTNI GGTCTC 2 cut(s) 321, 393
BsrI ACTGG 5 cut(s) 657, 1327, 1915, 2012, 2423
BssMI GATC 6 cut(s) 74, 715, 823, 1789, 1936, 2370
BssNI GRCGYC 5 cut(s) 152, 158, 255, 1224, 2158
BssT1I CCWWGG 8 cut(s) 40, 513, 681, 780, 972, 1578, 2079, 2184
Bst2UI CCWGG 1 cut(s) 176
Bst4CI ACNGT 5 cut(s) 120, 1400, 1512, 1704, 2284
Bst6I CTCTTC 1 cut(s) 1946
BstACI GRCGYC 5 cut(s) 152, 158, 255, 1224, 2158
BstAPI GCANNNNNTGC 1 cut(s) 935
BstC8I GCNNGC 7 cut(s) 290, 579, 590, 834, 969, 1193, 1339
BstDEI CTNAG 8 cut(s) 404, 561, 798, 1067, 1971, 2052, 2100, 2145
BstDSI CCRYGG 7 cut(s) 40, 513, 780, 972, 1578, 2079, 2184
BstEII GGTNACC 1 cut(s) 2363
BstF5I GGATG 4 cut(s) 18, 1102, 1684, 1921
BstFNI CGCG 2 cut(s) 288, 1300
BstHHI GCGC 1 cut(s) 288
BstKTI GATC 6 cut(s) 77, 718, 826, 1792, 1939, 2373
BstMAI GTCTC 4 cut(s) 321, 393, 894, 1798
BstMBI GATC 6 cut(s) 74, 715, 823, 1789, 1936, 2370
BstMCI CGRYCG 1 cut(s) 475
BstMWI GCNNNNNNNGC 6 cut(s) 242, 335, 361, 370, 935, 2105
BstNI CCWGG 1 cut(s) 176
BstNSI RCATGY 3 cut(s) 1620, 2300, 2450
BstPI GGTNACC 1 cut(s) 2363
BstSCI CCNGG 1 cut(s) 174
BstSFI CTRYAG 1 cut(s) 2109
BstUI CGCG 2 cut(s) 288, 1300
BstV1I GCAGC 9 cut(s) 376, 379, 382, 1576, 1701, 1823, 1915, 2095, 2237
BstV2I GAAGAC 1 cut(s) 526
Bsu15I ATCGAT 1 cut(s) 263
BsuRI GGCC 8 cut(s) 141, 180, 381, 468, 581, 1191, 1449, 1695
BsuTUI ATCGAT 1 cut(s) 263
BtgI CCRYGG 7 cut(s) 40, 513, 780, 972, 1578, 2079, 2184
BtsCI GGATG 4 cut(s) 18, 1102, 1684, 1921
BtsIMutI CAGTG 2 cut(s) 664, 2328
BveI ACCTGC 1 cut(s) 1062
Cac8I GCNNGC 7 cut(s) 290, 579, 590, 834, 969, 1193, 1339
CciI TCATGA 1 cut(s) 2092
CfoI GCGC 1 cut(s) 288
Cfr13I GGNCC 7 cut(s) 139, 178, 379, 451, 517, 883, 2010
ClaI ATCGAT 1 cut(s) 263
CseI GACGC 5 cut(s) 160, 166, 263, 1213, 2147
Csp6I GTAC 4 cut(s) 346, 1887, 2056, 2473
CviQI GTAC 4 cut(s) 346, 1887, 2056, 2473
DdeI CTNAG 8 cut(s) 404, 561, 798, 1067, 1971, 2052, 2100, 2145
DpnI GATC 6 cut(s) 76, 717, 825, 1791, 1938, 2372
DpnII GATC 6 cut(s) 74, 715, 823, 1789, 1936, 2370
DraIII CACNNNGTG 2 cut(s) 935, 1598
EaeI YGGCCR 1 cut(s) 1447
Eam1104I CTCTTC 1 cut(s) 1946
EarI CTCTTC 1 cut(s) 1946
EciI GGCGGA 1 cut(s) 344
Eco130I CCWWGG 8 cut(s) 40, 513, 681, 780, 972, 1578, 2079, 2184
Eco147I AGGCCT 2 cut(s) 581, 1191
Eco31I GGTCTC 2 cut(s) 321, 393
Eco32I GATATC 1 cut(s) 2122
Eco47I GGWCC 4 cut(s) 451, 517, 883, 2010
Eco57I CTGAAG 1 cut(s) 942
Eco88I CYCGRG 1 cut(s) 2069
Eco91I GGTNACC 1 cut(s) 2363
EcoO109I RGGNCCY 1 cut(s) 379
EcoO65I GGTNACC 1 cut(s) 2363
EcoRI GAATTC 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 174
EcoRV GATATC 1 cut(s) 2122
EcoT14I CCWWGG 8 cut(s) 40, 513, 681, 780, 972, 1578, 2079, 2184
ErhI CCWWGG 8 cut(s) 40, 513, 681, 780, 972, 1578, 2079, 2184
Esp3I CGTCTC 1 cut(s) 894
FalI AAGNNNNNCTT 2 cut(s) 387, 419
FaqI GGGAC 4 cut(s) 129, 1159, 1355, 1877
FbaI TGATCA 2 cut(s) 823, 1789
FblI GTMKAC 1 cut(s) 417
FokI GGATG 4 cut(s) 5, 1109, 1691, 1928
FspBI CTAG 8 cut(s) 1106, 1187, 1338, 1568, 1697, 1817, 2390, 2476
GlaI GCGC 1 cut(s) 287
GsuI CTGGAG 4 cut(s) 368, 1344, 1457, 2146
HaeIII GGCC 8 cut(s) 141, 180, 381, 468, 581, 1191, 1449, 1695
HapII CCGG 1 cut(s) 438
HgaI GACGC 5 cut(s) 160, 166, 263, 1213, 2147
HhaI GCGC 1 cut(s) 288
Hin1I GRCGYC 5 cut(s) 152, 158, 255, 1224, 2158
Hin6I GCGC 1 cut(s) 286
HinP1I GCGC 1 cut(s) 286
HincII GTYRAC 2 cut(s) 418, 1832
HindII GTYRAC 2 cut(s) 418, 1832
HindIII AAGCTT 2 cut(s) 563, 2204
HinfI GANTC 9 cut(s) 88, 248, 265, 477, 1062, 1368, 1609, 2074, 2318
HpaII CCGG 1 cut(s) 438
HphI GGTGA 5 cut(s) 370, 452, 1610, 1910, 2375
Hpy166II GTNNAC 3 cut(s) 418, 1832, 2338
Hpy8I GTNNAC 3 cut(s) 418, 1832, 2338
Hpy99I CGWCG 4 cut(s) 160, 166, 228, 310
HpyAV CCTTC 6 cut(s) 454, 1087, 1716, 2058, 2110, 2469
HpyCH4III ACNGT 5 cut(s) 120, 1400, 1512, 1704, 2284
HpyCH4IV ACGT 3 cut(s) 531, 903, 1056
HpyF10VI GCNNNNNNNGC 6 cut(s) 242, 335, 361, 370, 935, 2105
HpyF3I CTNAG 8 cut(s) 404, 561, 798, 1067, 1971, 2052, 2100, 2145
HpySE526I ACGT 3 cut(s) 531, 903, 1056
Hsp92I GRCGYC 5 cut(s) 152, 158, 255, 1224, 2158
HspAI GCGC 1 cut(s) 286
KpnI GGTACC 1 cut(s) 1890
Ksp22I TGATCA 2 cut(s) 823, 1789
Kzo9I GATC 6 cut(s) 74, 715, 823, 1789, 1936, 2370
LmnI GCTCC 5 cut(s) 15, 352, 508, 1438, 2173
Lsp1109I GCAGC 9 cut(s) 376, 379, 382, 1576, 1701, 1823, 1915, 2095, 2237
LweI GCATC 4 cut(s) 1111, 1324, 1993, 2124
MaeI CTAG 8 cut(s) 1106, 1187, 1338, 1568, 1697, 1817, 2390, 2476
MaeII ACGT 3 cut(s) 531, 903, 1056
MaeIII GTNAC 4 cut(s) 407, 599, 1776, 2363
MalI GATC 6 cut(s) 76, 717, 825, 1791, 1938, 2372
MboI GATC 6 cut(s) 74, 715, 823, 1789, 1936, 2370
MfeI CAATTG 1 cut(s) 990
MlsI TGGCCA 1 cut(s) 1449
MluNI TGGCCA 1 cut(s) 1449
MlyI GAGTC 4 cut(s) 97, 257, 1056, 1377
MmeI TCCRAC 5 cut(s) 231, 288, 1039, 1873, 1900
Mox20I TGGCCA 1 cut(s) 1449
MroXI GAANNNNTTC 1 cut(s) 2385
MscI TGGCCA 1 cut(s) 1449
MseI TTAA 6 cut(s) 489, 573, 860, 890, 1425, 2198
MslI CAYNNNNRTG 1 cut(s) 659
Msp20I TGGCCA 1 cut(s) 1449
MspA1I CMGCKG 1 cut(s) 1714
MspI CCGG 1 cut(s) 438
MspR9I CCNGG 1 cut(s) 176
MunI CAATTG 1 cut(s) 990
Mva1269I GAATGC 2 cut(s) 1660, 1829
MvaI CCWGG 1 cut(s) 176
MvnI CGCG 2 cut(s) 288, 1300
MwoI GCNNNNNNNGC 6 cut(s) 242, 335, 361, 370, 935, 2105
NcoI CCATGG 7 cut(s) 40, 513, 780, 972, 1578, 2079, 2184
NdeII GATC 6 cut(s) 74, 715, 823, 1789, 1936, 2370
NheI GCTAGC 1 cut(s) 1337
NlaIV GGNNCC 5 cut(s) 140, 380, 519, 1888, 2175
NmeAIII GCCGAG 1 cut(s) 1370
NmuCI GTSAC 2 cut(s) 1776, 2363
NspI RCATGY 3 cut(s) 1620, 2300, 2450
OliI CACNNNNGTG 1 cut(s) 659
PaeR7I CTCGAG 1 cut(s) 2069
PagI TCATGA 1 cut(s) 2092
PceI AGGCCT 2 cut(s) 581, 1191
PciI ACATGT 1 cut(s) 2296
PcsI WCGNNNNNNNCGW 3 cut(s) 83, 158, 477
PctI GAATGC 2 cut(s) 1660, 1829
PdmI GAANNNNTTC 1 cut(s) 2385
PfeI GAWTC 5 cut(s) 265, 477, 1609, 2074, 2318
PflMI CCANNNNNTGG 2 cut(s) 41, 663
PleI GAGTC 4 cut(s) 96, 256, 1056, 1376
PpsI GAGTC 4 cut(s) 96, 256, 1056, 1376
PscI ACATGT 1 cut(s) 2296
PsiI TTATAA 1 cut(s) 1670
Psp1406I AACGTT 1 cut(s) 1056
Psp6I CCWGG 1 cut(s) 174
PspEI GGTNACC 1 cut(s) 2363
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 5 cut(s) 140, 380, 519, 1888, 2175
PspPI GGNCC 7 cut(s) 139, 178, 379, 451, 517, 883, 2010
PsrI GAACNNNNNNTAC 2 cut(s) 1983, 2015
PstI CTGCAG 1 cut(s) 2113
PvuII CAGCTG 1 cut(s) 1714
RsaI GTAC 4 cut(s) 347, 1888, 2057, 2474
RsaNI GTAC 4 cut(s) 346, 1887, 2056, 2473
RseI CAYNNNNRTG 1 cut(s) 659
SalI GTCGAC 1 cut(s) 416
SaqAI TTAA 6 cut(s) 489, 573, 860, 890, 1425, 2198
Sau3AI GATC 6 cut(s) 74, 715, 823, 1789, 1936, 2370
Sau96I GGNCC 7 cut(s) 139, 178, 379, 451, 517, 883, 2010
ScaI AGTACT 2 cut(s) 347, 2474
SchI GAGTC 4 cut(s) 97, 257, 1056, 1377
ScrFI CCNGG 1 cut(s) 176
SfaNI GCATC 4 cut(s) 1111, 1324, 1993, 2124
SfcI CTRYAG 1 cut(s) 2109
Sfr274I CTCGAG 1 cut(s) 2069
SinI GGWCC 4 cut(s) 451, 517, 883, 2010
SlaI CTCGAG 1 cut(s) 2069
SmiMI CAYNNNNRTG 1 cut(s) 659
SmlI CTYRAG 1 cut(s) 2069
SmoI CTYRAG 1 cut(s) 2069
SseBI AGGCCT 2 cut(s) 581, 1191
SsiI CCGC 7 cut(s) 142, 233, 329, 886, 957, 1298, 2155
SspI AATATT 1 cut(s) 1556
SspMI CTAG 8 cut(s) 1106, 1187, 1338, 1568, 1697, 1817, 2390, 2476
StuI AGGCCT 2 cut(s) 581, 1191
StyD4I CCNGG 1 cut(s) 174
StyI CCWWGG 8 cut(s) 40, 513, 681, 780, 972, 1578, 2079, 2184
TaaI ACNGT 5 cut(s) 120, 1400, 1512, 1704, 2284
TaiI ACGT 3 cut(s) 534, 906, 1059
TaqI TCGA 9 cut(s) 77, 212, 263, 417, 475, 495, 1987, 2070, 2428
TaqII GACCGA 1 cut(s) 461
TatI WGTACW 3 cut(s) 345, 2055, 2472
TauI GCSGC 3 cut(s) 144, 236, 2158
TfiI GAWTC 5 cut(s) 265, 477, 1609, 2074, 2318
Tru1I TTAA 6 cut(s) 489, 573, 860, 890, 1425, 2198
Tru9I TTAA 6 cut(s) 489, 573, 860, 890, 1425, 2198
TscAI CASTG 2 cut(s) 664, 2328
TseFI GTSAC 2 cut(s) 1776, 2363
TseI GCWGC 9 cut(s) 364, 367, 370, 1564, 1714, 1811, 1903, 2108, 2225
Tsp45I GTSAC 2 cut(s) 1776, 2363
TspDTI ATGAA 9 cut(s) 526, 829, 1221, 1239, 2042, 2081, 2109, 2291, 2303
TspGWI ACGGA 4 cut(s) 422, 795, 923, 2002
TspRI CASTG 2 cut(s) 664, 2328
Van91I CCANNNNNTGG 2 cut(s) 41, 663
VpaK11BI GGWCC 4 cut(s) 451, 517, 883, 2010
XapI RAATTY 6 cut(s) 279, 491, 1586, 2149, 2190, 2344
XbaI TCTAGA 3 cut(s) 1105, 1816, 2389
XceI RCATGY 3 cut(s) 1620, 2300, 2450
XcmI CCANNNNNNNNNTGG 1 cut(s) 703
XhoI CTCGAG 1 cut(s) 2069
XmiI GTMKAC 1 cut(s) 417
XmnI GAANNNNTTC 1 cut(s) 2385
XspI CTAG 8 cut(s) 1106, 1187, 1338, 1568, 1697, 1817, 2390, 2476
ZrmI AGTACT 2 cut(s) 347, 2474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.