RchiOBHm_Chr2g0176681

CarD-like/TRCF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0015231
Physical Location & Seq
Forward (+)
88383205 .. 88389647
6443 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54368

Sequence Viewer

Length: 2460 bp
ATGGCTCTGCTCCTCCCCATCCCAATCCCAATCCATGGCGTTTCTTCTTGTTATAGGTCGAGGTCGGCGAGTCGGCTGTGCAGGGTTTGGGGTTGGGGACTGTTGAAAATCCAGAGAGGGGGCCGCACCCAGATGCCGACGCCGACGAAGAACAGCCTGGGCCTTTCCAATTCCACTTTCACGAAAACCAAATCGAGCAAAAAGCGACGGGAGGCGGCTGTGGAGGCAGAGTCGGACGCCATATCGATTCTAAACGAGAGAATTCGGCGCGAGCAGAGAAAGAAATCGTCGGAGGGGTCTCCGATGGAGGCGGAGGAGGCAGACAAGTACTTGGAGCTGGTGAAGCAGCAGCAGCAGAGGGGCCTCCAGAAGCTCAAAGGAGACCTTAGTTACAAGGTCGACCCCTACACTCTTCGTTCCGGTGATTATGTGGTCCATAAGAAGGTCGGCCTCGGTCGATTCGTTGGGATTAAATTCGACAATGCTCCTGCTTCCATGGACCCTATTGAATACGTCTTCATTGAGTATGCTGATGGCATGGCTAAGCTTCCTGTTAAGCAGGCCTCTCGCTTGCTCTATCGTTACAATCTCCCCAACGAAAACAAAAAACCTCACACATTGAGCAAACTGAACGACACCAGTGTGTGGGAGAGAAGAAAGACCAAGGGAAAGATTGCCATTCAGAAAATGGTTGTTGATCTGATGGAGTTGTATTTACACAGGCTCAAACAAACACGCCCTCCCTACCCTCTTACTCCTGCCATGGCTCACTTTGTTTCTCAGTTTCCGTATGAGCCTACTCCTGATCAAAAGCAGGCTTTCATTGATGTTGAGAAAGATTTAACAGGGAGGGAAACTCCAATGGACCGCTTAATTTGTGGAGACGTTGGCTTTGGCAAAACTGAAGTTGCACTCCGTGCCATCTTTTGTGTAGTTTCCGCAGGGAAGCAAGCCATGGTTCTTGCACCCACAATTGTTTTAGCCAAGCAACATTTTGAAGTTATCTCACAGCGATTTTCTATCTACCCAAATATCAACGTTGGACTCCTTAGCAGGTTTCAGACTAAATCAGAGAAAGATGAGCATCTAGACATGATAAAGAATGGTCATTTGGACATAATTGTTGGGACACACTCACTTCTTGGAAGTCGTGTTGTGTATAATAATCTAGGCCTGCTTGTCGTTGATGAAGAACAGAGGTTTGGCGTCAAACAGAAAGAGAAGATTGCTTCATTCAAAACATCAGTTGATGTTCTTACTCTATCTGCAACACCTATACCGCGAACCCTTTATTTAGCATTGACTGGTTTTCGTGATGCTAGCTTGATTTCAACTCCACCTCCAGAAAGAGTCCCTATAAGAACCCATCTCTCGGCATACAGTAAAGAAAGGGTATTATCAGCAATTAAGTATGAGCTGGAGCGAGGTGGCCAAGTATTTTATGTTTTGCCTCGTATCAAAGGACTTGAAGAAGTGATGAGTTTTCTTGAACAGTCGTTTCCTGATGTTGAAATAGCTATTGCACACGGGAAGCAATATTCAAAGCAGCTAGAGGAAACCATGGAGAAATTTTCACAAGGTGAAATCAAGATTCTTACATGCACTAATATAGTAGAAAGTGGGCTTGACATTCAGAATGCAAACACCATTATAATTCAGGATGTTCAACAATTTGGCCTAGCACAGTTGTATCAGCTGCGGGGAAGGGTGGGGAGGGCAGATAAGGAAGCTCACGCACACTTATTTTATCCAGAGAAGTCACTTCTAACTGATCAGGCATTGGAGAGACTTGCAGCTCTAGAGGAATGCTGTCAACTTGGGCAAGGTTTCCAACTTGCAGAGAGGGACATGGGAATAAGAGGCTTTGGTACCATCTTTGGTGAGCAGCAGACTGGGGATGTTGGAAATGTTGGCATTGATCTCTTCTTTGAAATGCTGTTTGAGAGCTTGTCTAAGGTTGATGAGCATCGAGTAGTTTCAGTTCCTTACTGGTCCGTTGAGATTGATATGAATATAAATCCTCATCTCCCTTCTGAGTACATAAATAATCTCGAGAATCCCATGGAGATAATTCATGAAGCTGAGAAAGCTGCAGAGAAGGATATCTGGAGTTTGATGCAATATACTGAGAATTTGCGGCGTCAATATGGAAAGGAGCCACACTCCATGGAAATTTTATTAAAGAAGCTTTATGTGAGGAGAATGGCAGCAGACATTGGAATAACCAAGATATATGCTTCTGGAAAGATGGTTTTTATGAAAACAGTTATGAATAAACATGTTTTCAATCTGATAACAGATTCAGTGGTTTCTGATGTCCACCGAAATTCTTTGGTATTTGAGGGTGACCAGATCAAGGCAGAACTTCTTCTAGAATTGCCAAGAGAACAACTCCTAAACTGGATATTTCGATGTTTGGCTGAACTACATGCTTCCTTACCTTCCCTCATAAAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarD_CdnL_TRCF DEAD Helicase_C TRCF

Protein Analysis

819

Amino Acids

93.0

Weight (kDa)

8.78

Isoelectric Point (pI)

35.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CarD_TRCF_RID PF02559 138 - 195 2.1e-09 CarD-like/TRCF RID domain
ResIII PF04851 265 - 425 3.3e-09 Type III restriction enzyme, res subunit
DEAD PF00270 267 - 429 1.7e-23 DEAD/DEAH box helicase
Helicase_C PF00271 469 - 573 2.2e-20 Helicase conserved C-terminal domain
TRCF PF03461 671 - 757 1.3e-06 TRCF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02060 AT3G02060 AT3G02060
fragaria_vesca FvH4_6g54430
malus_domestica MD17G1004500.v1.1
prunus_persica Prupe.3G314900_v2.0.a1
pyrus_communis pycom111g00110 pycom17g00110
rosa_chinensis RchiOBHm_Chr2g0176681
rosa_laevigata RLG00000022435
rosa_rugosa Rorug02G0597500
rosa_samantha Rh2BG686000 Rh2CG649300 Rh2DG700200
rosa_wichuraiana Rw0G015680 Rw2G055390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1652
Acc36I ACCTGC 1 cut(s) 1044
Acc65I GGTACC 1 cut(s) 1868
AccB1I GGYRCC 1 cut(s) 1868
AccB7I CCANNNNNTGG 2 cut(s) 35, 645
AccI GTMKAC 1 cut(s) 399
AccII CGCG 2 cut(s) 270, 1282
AciI CCGC 8 cut(s) 124, 215, 311, 868, 939, 1280, 1699, 2137
AclI AACGTT 1 cut(s) 1038
AcoI YGGCCR 1 cut(s) 1429
AcsI RAATTY 6 cut(s) 261, 473, 1568, 2131, 2172, 2326
AcuI CTGAAG 1 cut(s) 924
AcyI GRCGYC 4 cut(s) 140, 237, 1206, 2140
AdeI CACNNNGTG 2 cut(s) 917, 1580
AfaI GTAC 4 cut(s) 329, 1870, 2039, 2456
AfiI CCNNNNNNNGG 6 cut(s) 35, 118, 442, 645, 1372, 2356
AflIII ACRYGT 1 cut(s) 2278
AjnI CCWGG 1 cut(s) 156
AjuI GAANNNNNNNTTGG 6 cut(s) 161, 193, 1094, 1126, 1185, 1217
AleI CACNNNNGTG 1 cut(s) 641
Alw26I GTCTC 4 cut(s) 303, 375, 876, 1780
Ama87I CYCGRG 1 cut(s) 2051
AoxI GGCC 8 cut(s) 121, 160, 361, 448, 561, 1171, 1429, 1675
ApeKI GCWGC 9 cut(s) 346, 349, 352, 1546, 1696, 1793, 1885, 2090, 2207
ApoI RAATTY 6 cut(s) 261, 473, 1568, 2131, 2172, 2326
ArsI GACNNNNNNTTYG 2 cut(s) 875, 907
Asp700I GAANNNNTTC 1 cut(s) 2367
Asp718I GGTACC 1 cut(s) 1868
AspLEI GCGC 1 cut(s) 270
AspS9I GGNCC 7 cut(s) 121, 160, 361, 433, 499, 865, 1992
AsuHPI GGTGA 5 cut(s) 352, 434, 1592, 1892, 2357
AsuNHI GCTAGC 1 cut(s) 1319
AvaI CYCGRG 1 cut(s) 2051
AvaII GGWCC 4 cut(s) 433, 499, 865, 1992
BaeI ACNNNNGTAYC 2 cut(s) 1673, 1706
BalI TGGCCA 1 cut(s) 1431
BanI GGYRCC 1 cut(s) 1868
BbsI GAAGAC 1 cut(s) 508
BbvI GCAGC 9 cut(s) 358, 361, 364, 1558, 1683, 1805, 1897, 2077, 2219
BccI CCATC 8 cut(s) 26, 298, 527, 697, 929, 1374, 1880, 2242
BcgI CGANNNNNNTGC 2 cut(s) 548, 582
BciT130I CCWGG 1 cut(s) 158
BclI TGATCA 2 cut(s) 805, 1771
BcoDI GTCTC 4 cut(s) 303, 375, 876, 1780
BfaI CTAG 8 cut(s) 1088, 1169, 1320, 1550, 1679, 1799, 2372, 2458
BfmI CTRYAG 1 cut(s) 2091
BfuAI ACCTGC 1 cut(s) 1044
BlpI GCTNAGC 1 cut(s) 543
BmcAI AGTACT 2 cut(s) 329, 2456
Bme1390I CCNGG 1 cut(s) 158
Bme18I GGWCC 4 cut(s) 433, 499, 865, 1992
BmeT110I CYCGRG 1 cut(s) 2051
BmgT120I GGNCC 7 cut(s) 121, 160, 361, 433, 499, 865, 1992
BmiI GGNNCC 5 cut(s) 122, 362, 501, 1870, 2157
BmrFI CCNGG 1 cut(s) 158
BmrI ACTGGG 1 cut(s) 1902
BmsI GCATC 5 cut(s) 123, 1093, 1306, 1975, 2106
BmtI GCTAGC 1 cut(s) 1323
BmuI ACTGGG 1 cut(s) 1902
BpiI GAAGAC 1 cut(s) 508
BplI GAGNNNNNCTC 6 cut(s) 841, 873, 2147, 2179, 2376, 2408
BpmI CTGGAG 4 cut(s) 350, 1326, 1439, 2128
Bpu10I CCTNAGC 1 cut(s) 1049
Bpu1102I GCTNAGC 1 cut(s) 543
Bsa29I ATCGAT 1 cut(s) 245
BsaBI GATNNNNATC 2 cut(s) 1083, 1965
BsaHI GRCGYC 4 cut(s) 140, 237, 1206, 2140
BsaI GGTCTC 2 cut(s) 303, 375
BsaWI WCCGGW 1 cut(s) 419
BsaXI ACNNNNNCTCC 6 cut(s) 215, 245, 724, 754, 1324, 1354
Bsc4I CCNNNNNNNGG 6 cut(s) 35, 118, 442, 645, 1372, 2356
Bse1I ACTGG 5 cut(s) 639, 1309, 1897, 1994, 2405
Bse8I GATNNNNATC 2 cut(s) 1083, 1965
BseBI CCWGG 1 cut(s) 158
BseCI ATCGAT 1 cut(s) 245
BseGI GGATG 3 cut(s) 18, 1666, 1903
BseJI GATNNNNATC 2 cut(s) 1083, 1965
BseLI CCNNNNNNNGG 6 cut(s) 35, 118, 442, 645, 1372, 2356
BseMII CTCAG 4 cut(s) 794, 2025, 2073, 2118
BseNI ACTGG 5 cut(s) 639, 1309, 1897, 1994, 2405
BseRI GAGGAG 2 cut(s) 329, 2212
BseXI GCAGC 9 cut(s) 358, 361, 364, 1558, 1683, 1805, 1897, 2077, 2219
BsgI GTGCAG 1 cut(s) 100
Bsh1236I CGCG 2 cut(s) 270, 1282
Bsh1285I CGRYCG 1 cut(s) 457
BshFI GGCC 8 cut(s) 123, 162, 363, 450, 563, 1173, 1431, 1677
BshNI GGYRCC 1 cut(s) 1868
BshVI ATCGAT 1 cut(s) 245
BsiEI CGRYCG 1 cut(s) 457
BsiHKCI CYCGRG 1 cut(s) 2051
BsiSI CCGG 1 cut(s) 420
BslFI GGGAC 4 cut(s) 111, 1141, 1337, 1859
BslI CCNNNNNNNGG 6 cut(s) 35, 118, 442, 645, 1372, 2356
BsmAI GTCTC 4 cut(s) 303, 375, 876, 1780
BsmBI CGTCTC 1 cut(s) 876
BsmFI GGGAC 4 cut(s) 111, 1141, 1337, 1859
BsmI GAATGC 2 cut(s) 1642, 1811
BsnI GGCC 8 cut(s) 123, 162, 363, 450, 563, 1173, 1431, 1677
Bso31I GGTCTC 2 cut(s) 303, 375
BsoBI CYCGRG 1 cut(s) 2051
Bsp143I GATC 5 cut(s) 697, 805, 1771, 1918, 2352
Bsp1720I GCTNAGC 1 cut(s) 543
Bsp19I CCATGG 7 cut(s) 34, 495, 762, 954, 1560, 2061, 2166
BspACI CCGC 8 cut(s) 124, 215, 311, 868, 939, 1280, 1699, 2137
BspANI GGCC 8 cut(s) 123, 162, 363, 450, 563, 1173, 1431, 1677
BspCNI CTCAG 4 cut(s) 793, 2026, 2074, 2119
BspDI ATCGAT 1 cut(s) 245
BspFNI CGCG 2 cut(s) 270, 1282
BspHI TCATGA 1 cut(s) 2074
BspLI GGNNCC 5 cut(s) 122, 362, 501, 1870, 2157
BspMAI CTGCAG 1 cut(s) 2095
BspMI ACCTGC 1 cut(s) 1044
BspOI GCTAGC 1 cut(s) 1323
BspT107I GGYRCC 1 cut(s) 1868
BspTNI GGTCTC 2 cut(s) 303, 375
BsrI ACTGG 5 cut(s) 639, 1309, 1897, 1994, 2405
BssMI GATC 5 cut(s) 697, 805, 1771, 1918, 2352
BssNI GRCGYC 4 cut(s) 140, 237, 1206, 2140
BssT1I CCWWGG 8 cut(s) 34, 495, 663, 762, 954, 1560, 2061, 2166
Bst2UI CCWGG 1 cut(s) 158
Bst4CI ACNGT 5 cut(s) 102, 1382, 1494, 1686, 2266
Bst6I CTCTTC 2 cut(s) 417, 1928
BstACI GRCGYC 4 cut(s) 140, 237, 1206, 2140
BstAPI GCANNNNNTGC 1 cut(s) 917
BstC8I GCNNGC 7 cut(s) 272, 561, 572, 816, 951, 1175, 1321
BstDEI CTNAG 8 cut(s) 386, 543, 780, 1049, 1953, 2034, 2082, 2127
BstDSI CCRYGG 7 cut(s) 34, 495, 762, 954, 1560, 2061, 2166
BstEII GGTNACC 1 cut(s) 2345
BstF5I GGATG 3 cut(s) 18, 1666, 1903
BstFNI CGCG 2 cut(s) 270, 1282
BstHHI GCGC 1 cut(s) 270
BstKTI GATC 5 cut(s) 700, 808, 1774, 1921, 2355
BstMAI GTCTC 4 cut(s) 303, 375, 876, 1780
BstMBI GATC 5 cut(s) 697, 805, 1771, 1918, 2352
BstMCI CGRYCG 1 cut(s) 457
BstMWI GCNNNNNNNGC 6 cut(s) 224, 317, 343, 352, 917, 2087
BstNI CCWGG 1 cut(s) 158
BstNSI RCATGY 3 cut(s) 1602, 2282, 2432
BstPI GGTNACC 1 cut(s) 2345
BstSCI CCNGG 1 cut(s) 156
BstSFI CTRYAG 1 cut(s) 2091
BstUI CGCG 2 cut(s) 270, 1282
BstV1I GCAGC 9 cut(s) 358, 361, 364, 1558, 1683, 1805, 1897, 2077, 2219
BstV2I GAAGAC 1 cut(s) 508
Bsu15I ATCGAT 1 cut(s) 245
BsuRI GGCC 8 cut(s) 123, 162, 363, 450, 563, 1173, 1431, 1677
BsuTUI ATCGAT 1 cut(s) 245
BtgI CCRYGG 7 cut(s) 34, 495, 762, 954, 1560, 2061, 2166
BtsCI GGATG 3 cut(s) 18, 1666, 1903
BtsIMutI CAGTG 2 cut(s) 646, 2310
BveI ACCTGC 1 cut(s) 1044
Cac8I GCNNGC 7 cut(s) 272, 561, 572, 816, 951, 1175, 1321
CciI TCATGA 1 cut(s) 2074
CfoI GCGC 1 cut(s) 270
Cfr13I GGNCC 7 cut(s) 121, 160, 361, 433, 499, 865, 1992
ClaI ATCGAT 1 cut(s) 245
CseI GACGC 4 cut(s) 148, 245, 1195, 2129
Csp6I GTAC 4 cut(s) 328, 1869, 2038, 2455
CviQI GTAC 4 cut(s) 328, 1869, 2038, 2455
DdeI CTNAG 8 cut(s) 386, 543, 780, 1049, 1953, 2034, 2082, 2127
DpnI GATC 5 cut(s) 699, 807, 1773, 1920, 2354
DpnII GATC 5 cut(s) 697, 805, 1771, 1918, 2352
DraIII CACNNNGTG 2 cut(s) 917, 1580
EaeI YGGCCR 1 cut(s) 1429
Eam1104I CTCTTC 2 cut(s) 417, 1928
EarI CTCTTC 2 cut(s) 417, 1928
EciI GGCGGA 1 cut(s) 326
Eco130I CCWWGG 8 cut(s) 34, 495, 663, 762, 954, 1560, 2061, 2166
Eco147I AGGCCT 2 cut(s) 563, 1173
Eco31I GGTCTC 2 cut(s) 303, 375
Eco32I GATATC 1 cut(s) 2104
Eco47I GGWCC 4 cut(s) 433, 499, 865, 1992
Eco57I CTGAAG 1 cut(s) 924
Eco88I CYCGRG 1 cut(s) 2051
Eco91I GGTNACC 1 cut(s) 2345
EcoO109I RGGNCCY 1 cut(s) 361
EcoO65I GGTNACC 1 cut(s) 2345
EcoRI GAATTC 1 cut(s) 261
EcoRII CCWGG 1 cut(s) 156
EcoRV GATATC 1 cut(s) 2104
EcoT14I CCWWGG 8 cut(s) 34, 495, 663, 762, 954, 1560, 2061, 2166
ErhI CCWWGG 8 cut(s) 34, 495, 663, 762, 954, 1560, 2061, 2166
Esp3I CGTCTC 1 cut(s) 876
FalI AAGNNNNNCTT 2 cut(s) 369, 401
FaqI GGGAC 4 cut(s) 111, 1141, 1337, 1859
FauI CCCGC 1 cut(s) 1692
FbaI TGATCA 2 cut(s) 805, 1771
FblI GTMKAC 1 cut(s) 399
FokI GGATG 3 cut(s) 5, 1673, 1910
FspBI CTAG 8 cut(s) 1088, 1169, 1320, 1550, 1679, 1799, 2372, 2458
GlaI GCGC 1 cut(s) 269
GsuI CTGGAG 4 cut(s) 350, 1326, 1439, 2128
HaeIII GGCC 8 cut(s) 123, 162, 363, 450, 563, 1173, 1431, 1677
HapII CCGG 1 cut(s) 420
HgaI GACGC 4 cut(s) 148, 245, 1195, 2129
HhaI GCGC 1 cut(s) 270
Hin1I GRCGYC 4 cut(s) 140, 237, 1206, 2140
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HincII GTYRAC 2 cut(s) 400, 1814
HindII GTYRAC 2 cut(s) 400, 1814
HindIII AAGCTT 2 cut(s) 545, 2186
HinfI GANTC 9 cut(s) 70, 230, 247, 459, 1044, 1350, 1591, 2056, 2300
HpaII CCGG 1 cut(s) 420
HphI GGTGA 5 cut(s) 352, 434, 1592, 1892, 2357
Hpy166II GTNNAC 3 cut(s) 400, 1814, 2320
Hpy8I GTNNAC 3 cut(s) 400, 1814, 2320
Hpy99I CGWCG 4 cut(s) 142, 148, 210, 292
HpyAV CCTTC 5 cut(s) 436, 1698, 2040, 2092, 2451
HpyCH4III ACNGT 5 cut(s) 102, 1382, 1494, 1686, 2266
HpyCH4IV ACGT 3 cut(s) 513, 885, 1038
HpyF10VI GCNNNNNNNGC 6 cut(s) 224, 317, 343, 352, 917, 2087
HpyF3I CTNAG 8 cut(s) 386, 543, 780, 1049, 1953, 2034, 2082, 2127
HpySE526I ACGT 3 cut(s) 513, 885, 1038
Hsp92I GRCGYC 4 cut(s) 140, 237, 1206, 2140
HspAI GCGC 1 cut(s) 268
KpnI GGTACC 1 cut(s) 1872
Ksp22I TGATCA 2 cut(s) 805, 1771
Kzo9I GATC 5 cut(s) 697, 805, 1771, 1918, 2352
LmnI GCTCC 5 cut(s) 15, 334, 490, 1420, 2155
Lsp1109I GCAGC 9 cut(s) 358, 361, 364, 1558, 1683, 1805, 1897, 2077, 2219
LweI GCATC 5 cut(s) 123, 1093, 1306, 1975, 2106
MaeI CTAG 8 cut(s) 1088, 1169, 1320, 1550, 1679, 1799, 2372, 2458
MaeII ACGT 3 cut(s) 513, 885, 1038
MaeIII GTNAC 4 cut(s) 389, 581, 1758, 2345
MalI GATC 5 cut(s) 699, 807, 1773, 1920, 2354
MboI GATC 5 cut(s) 697, 805, 1771, 1918, 2352
MfeI CAATTG 1 cut(s) 972
MlsI TGGCCA 1 cut(s) 1431
MluNI TGGCCA 1 cut(s) 1431
MlyI GAGTC 4 cut(s) 79, 239, 1038, 1359
MmeI TCCRAC 5 cut(s) 213, 270, 1021, 1855, 1882
Mox20I TGGCCA 1 cut(s) 1431
MroXI GAANNNNTTC 1 cut(s) 2367
MscI TGGCCA 1 cut(s) 1431
MseI TTAA 6 cut(s) 471, 555, 842, 872, 1407, 2180
MslI CAYNNNNRTG 2 cut(s) 131, 641
Msp20I TGGCCA 1 cut(s) 1431
MspA1I CMGCKG 1 cut(s) 1696
MspI CCGG 1 cut(s) 420
MspR9I CCNGG 1 cut(s) 158
MunI CAATTG 1 cut(s) 972
Mva1269I GAATGC 2 cut(s) 1642, 1811
MvaI CCWGG 1 cut(s) 158
MvnI CGCG 2 cut(s) 270, 1282
MwoI GCNNNNNNNGC 6 cut(s) 224, 317, 343, 352, 917, 2087
NcoI CCATGG 7 cut(s) 34, 495, 762, 954, 1560, 2061, 2166
NdeII GATC 5 cut(s) 697, 805, 1771, 1918, 2352
NheI GCTAGC 1 cut(s) 1319
NlaIV GGNNCC 5 cut(s) 122, 362, 501, 1870, 2157
NmeAIII GCCGAG 1 cut(s) 1352
NmuCI GTSAC 2 cut(s) 1758, 2345
NspI RCATGY 3 cut(s) 1602, 2282, 2432
OliI CACNNNNGTG 1 cut(s) 641
PaeR7I CTCGAG 1 cut(s) 2051
PagI TCATGA 1 cut(s) 2074
PceI AGGCCT 2 cut(s) 563, 1173
PciI ACATGT 1 cut(s) 2278
PcsI WCGNNNNNNNCGW 2 cut(s) 65, 459
PctI GAATGC 2 cut(s) 1642, 1811
PdmI GAANNNNTTC 1 cut(s) 2367
PfeI GAWTC 5 cut(s) 247, 459, 1591, 2056, 2300
PflMI CCANNNNNTGG 2 cut(s) 35, 645
PleI GAGTC 4 cut(s) 78, 238, 1038, 1358
PpsI GAGTC 4 cut(s) 78, 238, 1038, 1358
PscI ACATGT 1 cut(s) 2278
PsiI TTATAA 1 cut(s) 1652
Psp1406I AACGTT 1 cut(s) 1038
Psp6I CCWGG 1 cut(s) 156
PspEI GGTNACC 1 cut(s) 2345
PspGI CCWGG 1 cut(s) 156
PspN4I GGNNCC 5 cut(s) 122, 362, 501, 1870, 2157
PspPI GGNCC 7 cut(s) 121, 160, 361, 433, 499, 865, 1992
PsrI GAACNNNNNNTAC 2 cut(s) 1965, 1997
PstI CTGCAG 1 cut(s) 2095
PvuII CAGCTG 1 cut(s) 1696
RsaI GTAC 4 cut(s) 329, 1870, 2039, 2456
RsaNI GTAC 4 cut(s) 328, 1869, 2038, 2455
RseI CAYNNNNRTG 2 cut(s) 131, 641
SalI GTCGAC 1 cut(s) 398
SaqAI TTAA 6 cut(s) 471, 555, 842, 872, 1407, 2180
Sau3AI GATC 5 cut(s) 697, 805, 1771, 1918, 2352
Sau96I GGNCC 7 cut(s) 121, 160, 361, 433, 499, 865, 1992
ScaI AGTACT 2 cut(s) 329, 2456
SchI GAGTC 4 cut(s) 79, 239, 1038, 1359
ScrFI CCNGG 1 cut(s) 158
SfaNI GCATC 5 cut(s) 123, 1093, 1306, 1975, 2106
SfcI CTRYAG 1 cut(s) 2091
Sfr274I CTCGAG 1 cut(s) 2051
SinI GGWCC 4 cut(s) 433, 499, 865, 1992
SlaI CTCGAG 1 cut(s) 2051
SmiMI CAYNNNNRTG 2 cut(s) 131, 641
SmlI CTYRAG 1 cut(s) 2051
SmoI CTYRAG 1 cut(s) 2051
SseBI AGGCCT 2 cut(s) 563, 1173
SsiI CCGC 8 cut(s) 124, 215, 311, 868, 939, 1280, 1699, 2137
SspI AATATT 1 cut(s) 1538
SspMI CTAG 8 cut(s) 1088, 1169, 1320, 1550, 1679, 1799, 2372, 2458
StuI AGGCCT 2 cut(s) 563, 1173
StyD4I CCNGG 1 cut(s) 156
StyI CCWWGG 8 cut(s) 34, 495, 663, 762, 954, 1560, 2061, 2166
TaaI ACNGT 5 cut(s) 102, 1382, 1494, 1686, 2266
TaiI ACGT 3 cut(s) 516, 888, 1041
TaqI TCGA 9 cut(s) 59, 194, 245, 399, 457, 477, 1969, 2052, 2410
TaqII GACCGA 1 cut(s) 443
TatI WGTACW 3 cut(s) 327, 2037, 2454
TauI GCSGC 3 cut(s) 126, 218, 2140
TfiI GAWTC 5 cut(s) 247, 459, 1591, 2056, 2300
Tru1I TTAA 6 cut(s) 471, 555, 842, 872, 1407, 2180
Tru9I TTAA 6 cut(s) 471, 555, 842, 872, 1407, 2180
TscAI CASTG 2 cut(s) 646, 2310
TseFI GTSAC 2 cut(s) 1758, 2345
TseI GCWGC 9 cut(s) 346, 349, 352, 1546, 1696, 1793, 1885, 2090, 2207
Tsp45I GTSAC 2 cut(s) 1758, 2345
TspDTI ATGAA 9 cut(s) 508, 811, 1203, 1221, 2024, 2063, 2091, 2273, 2285
TspGWI ACGGA 3 cut(s) 777, 905, 1984
TspRI CASTG 2 cut(s) 646, 2310
Van91I CCANNNNNTGG 2 cut(s) 35, 645
VpaK11BI GGWCC 4 cut(s) 433, 499, 865, 1992
XapI RAATTY 6 cut(s) 261, 473, 1568, 2131, 2172, 2326
XbaI TCTAGA 3 cut(s) 1087, 1798, 2371
XceI RCATGY 3 cut(s) 1602, 2282, 2432
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XhoI CTCGAG 1 cut(s) 2051
XmiI GTMKAC 1 cut(s) 399
XmnI GAANNNNTTC 1 cut(s) 2367
XspI CTAG 8 cut(s) 1088, 1169, 1320, 1550, 1679, 1799, 2372, 2458
ZrmI AGTACT 2 cut(s) 329, 2456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.