pycom111g00110

CarD-like/TRCF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0015231
Physical Location & Seq
Reverse (-)
100765 .. 101524
760 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g00110.1

Sequence Viewer

Length: 615 bp
ATGCCGCTGCTTCTCCCAATCCCTGCCCTCAACTCTAGCTCTAGCTCGGCAATTCAAGGATTGAGGCTCAGCTCATGTCCCGGGGCCAGCCTTTGTTGGCGACTCGGAAGAAGACGACTGCATTGCTGTGTGCCCAATGCTGTCTACACCGCCCACACCCAAAGCACGGAACAGCATTGGAAACCGACATCGGGGAAGATAGAGGATTCGGCGGAGGGCGACGCCATATCCATTCTCAATGAAAGGATTCGGCGGGAGCAGAGCAAGAGAGAGGGCTCCGCAACGAGAACGGTGATGGAGGCGGAGGCGGCGTACAAATACGTAGAGATGGTGAAGCAGCAGCAGCAGAGGGGCCTCGAGAAGCTCCGAGGCGACGTTGGTTACAAGGTGGATCCCTACACTCTTCGTTCTGGGGACTACGTCATCCATAAGAAGGTTGGCATTGGTCGGTTCGTTGGGATTAAATTGGACACTACTAGTCCCAATTCCATTTCCAATTCCACTTCTAAGACCGACTCCATTGAATACGTCTTCATCGAGTATGCCGATGGCATGGCTAAGCTTCCTGTCAAGCAGGCCTCTCGCTTGCTCTATCGTTATAATCTCCCGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarD_CdnL_TRCF DEAD Helicase_C TRCF

Protein Analysis

205

Amino Acids

22.77

Weight (kDa)

9.45

Isoelectric Point (pI)

56.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CarD_TRCF_RID PF02559 135 - 200 8.6e-11 CarD-like/TRCF RID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015231)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02060 AT3G02060 AT3G02060
fragaria_vesca FvH4_6g54430
malus_domestica MD17G1004500.v1.1
prunus_persica Prupe.3G314900_v2.0.a1
pyrus_communis pycom111g00110 pycom17g00110
rosa_chinensis RchiOBHm_Chr2g0176681
rosa_laevigata RLG00000022435
rosa_rugosa Rorug02G0597500
rosa_samantha Rh2BG686000 Rh2CG649300 Rh2DG700200
rosa_wichuraiana Rw0G015680 Rw2G055390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 600
AccI GTMKAC 1 cut(s) 144
AciI CCGC 7 cut(s) 5, 150, 212, 253, 279, 302, 308
AclWI GGATC 2 cut(s) 386, 399
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 3 cut(s) 166, 191, 433
AgsI TTSAA 2 cut(s) 56, 524
AhlI ACTAGT 1 cut(s) 476
AluBI AGCT 5 cut(s) 39, 45, 72, 364, 562
AluI AGCT 5 cut(s) 39, 45, 72, 364, 562
AlwI GGATC 2 cut(s) 386, 399
Ama87I CYCGRG 2 cut(s) 80, 356
AoxI GGCC 3 cut(s) 84, 352, 576
ApeKI GCWGC 4 cut(s) 7, 337, 340, 343
Asp700I GAANNNNTTC 1 cut(s) 246
AspS9I GGNCC 2 cut(s) 84, 352
AsuC2I CCSGG 2 cut(s) 81, 82
AsuHPI GGTGA 2 cut(s) 304, 343
AvaI CYCGRG 2 cut(s) 80, 356
BaeGI GKGCMC 1 cut(s) 135
BamHI GGATCC 1 cut(s) 391
BanII GRGCYC 1 cut(s) 278
BbsI GAAGAC 2 cut(s) 118, 523
BbvI GCAGC 3 cut(s) 349, 352, 355
BccI CCATC 3 cut(s) 289, 322, 542
BcgI CGANNNNNNTGC 4 cut(s) 105, 139, 563, 597
BcnI CCSGG 2 cut(s) 81, 82
BcuI ACTAGT 1 cut(s) 476
BfaI CTAG 3 cut(s) 36, 42, 477
BisI GCNGC 6 cut(s) 5, 8, 309, 338, 341, 344
BlpI GCTNAGC 2 cut(s) 68, 558
BlsI GCNGC 6 cut(s) 6, 9, 310, 339, 342, 345
Bme1390I CCNGG 2 cut(s) 81, 82
BmeT110I CYCGRG 2 cut(s) 80, 356
BmgT120I GGNCC 2 cut(s) 84, 352
BmiI GGNNCC 4 cut(s) 85, 277, 353, 393
BmrFI CCNGG 2 cut(s) 81, 82
BpiI GAAGAC 2 cut(s) 118, 523
Bpu1102I GCTNAGC 2 cut(s) 68, 558
BpuMI CCSGG 2 cut(s) 81, 82
BsaAI YACGTR 1 cut(s) 322
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 3 cut(s) 80, 81, 367
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 191, 433
Bse3DI GCAATG 1 cut(s) 121
BseDI CCNNGG 3 cut(s) 80, 81, 367
BseGI GGATG 1 cut(s) 423
BseLI CCNNNNNNNGG 3 cut(s) 166, 191, 433
BseMI GCAATG 1 cut(s) 121
BseMII CTCAG 1 cut(s) 82
BseSI GKGCMC 1 cut(s) 135
BseXI GCAGC 3 cut(s) 349, 352, 355
BshFI GGCC 3 cut(s) 86, 354, 578
BsiHKCI CYCGRG 2 cut(s) 80, 356
BsiSI CCGG 1 cut(s) 81
BslFI GGGAC 3 cut(s) 63, 428, 465
BslI CCNNNNNNNGG 3 cut(s) 166, 191, 433
BsmFI GGGAC 3 cut(s) 63, 428, 465
BsnI GGCC 3 cut(s) 86, 354, 578
BsoBI CYCGRG 2 cut(s) 80, 356
Bsp1286I GDGCHC 2 cut(s) 135, 278
Bsp143I GATC 1 cut(s) 391
Bsp1720I GCTNAGC 2 cut(s) 68, 558
BspACI CCGC 7 cut(s) 5, 150, 212, 253, 279, 302, 308
BspANI GGCC 3 cut(s) 86, 354, 578
BspCNI CTCAG 1 cut(s) 81
BspLI GGNNCC 4 cut(s) 85, 277, 353, 393
BspPI GGATC 2 cut(s) 386, 399
BsrDI GCAATG 1 cut(s) 121
BssECI CCNNGG 3 cut(s) 80, 81, 367
BssMI GATC 1 cut(s) 391
BssNI GRCGYC 1 cut(s) 222
Bst4CI ACNGT 1 cut(s) 292
Bst6I CTCTTC 1 cut(s) 408
BstACI GRCGYC 1 cut(s) 222
BstBAI YACGTR 1 cut(s) 322
BstC8I GCNNGC 3 cut(s) 88, 576, 587
BstDEI CTNAG 3 cut(s) 68, 507, 558
BstF5I GGATG 1 cut(s) 423
BstKTI GATC 1 cut(s) 394
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 2 cut(s) 308, 343
BstSCI CCNGG 2 cut(s) 79, 80
BstSLI GKGCMC 1 cut(s) 135
BstSNI TACGTA 1 cut(s) 322
BstV1I GCAGC 3 cut(s) 349, 352, 355
BstV2I GAAGAC 2 cut(s) 118, 523
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
BsuRI GGCC 3 cut(s) 86, 354, 578
BtsCI GGATG 1 cut(s) 423
Cac8I GCNNGC 3 cut(s) 88, 576, 587
Cfr13I GGNCC 2 cut(s) 84, 352
Cfr9I CCCGGG 1 cut(s) 80
CseI GACGC 1 cut(s) 230
Csp6I GTAC 1 cut(s) 313
CviAII CATG 2 cut(s) 75, 553
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 3 cut(s) 68, 507, 558
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
EciI GGCGGA 2 cut(s) 227, 317
Eco105I TACGTA 1 cut(s) 322
Eco147I AGGCCT 1 cut(s) 578
Eco24I GRGCYC 1 cut(s) 278
Eco88I CYCGRG 2 cut(s) 80, 356
EcoO109I RGGNCCY 1 cut(s) 352
EcoT38I GRGCYC 1 cut(s) 278
FaeI CATG 2 cut(s) 78, 556
FaiI YATR 6 cut(s) 76, 227, 429, 543, 554, 600
FaqI GGGAC 3 cut(s) 63, 428, 465
FatI CATG 2 cut(s) 74, 552
FauI CCCGC 1 cut(s) 246
FblI GTMKAC 1 cut(s) 144
Fnu4HI GCNGC 6 cut(s) 5, 8, 309, 338, 341, 344
FokI GGATG 1 cut(s) 410
FriOI GRGCYC 1 cut(s) 278
Fsp4HI GCNGC 6 cut(s) 5, 8, 309, 338, 341, 344
FspBI CTAG 3 cut(s) 36, 42, 477
GluI GCNGC 6 cut(s) 5, 8, 309, 338, 341, 344
HaeIII GGCC 3 cut(s) 86, 354, 578
HapII CCGG 1 cut(s) 81
HgaI GACGC 1 cut(s) 230
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 2 cut(s) 78, 556
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 4 cut(s) 102, 206, 247, 515
HpaII CCGG 1 cut(s) 81
HphI GGTGA 2 cut(s) 304, 343
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 2 cut(s) 107, 368
Hpy188III TCNNGA 2 cut(s) 358, 607
Hpy8I GTNNAC 1 cut(s) 145
Hpy99I CGWCG 2 cut(s) 224, 377
HpyAV CCTTC 1 cut(s) 427
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4IV ACGT 4 cut(s) 321, 375, 420, 528
HpyCH4V TGCA 1 cut(s) 121
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 343
HpyF3I CTNAG 3 cut(s) 68, 507, 558
HpySE526I ACGT 4 cut(s) 321, 375, 420, 528
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 2 cut(s) 78, 556
Kzo9I GATC 1 cut(s) 391
LmnI GCTCC 3 cut(s) 256, 281, 369
LpnPI CCDG 6 cut(s) 36, 94, 100, 396, 560, 579
Lsp1109I GCAGC 3 cut(s) 349, 352, 355
MaeI CTAG 3 cut(s) 36, 42, 477
MaeII ACGT 4 cut(s) 321, 375, 420, 528
MaeIII GTNAC 1 cut(s) 380
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 5 cut(s) 120, 123, 208, 395, 523
MflI RGATCY 1 cut(s) 391
MhlI GDGCHC 2 cut(s) 135, 278
MluCI AATT 4 cut(s) 51, 464, 484, 496
MlyI GAGTC 2 cut(s) 96, 509
MroXI GAANNNNTTC 1 cut(s) 246
MseI TTAA 1 cut(s) 462
MslI CAYNNNNRTG 1 cut(s) 126
MspA1I CMGCKG 1 cut(s) 7
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 2 cut(s) 81, 82
MwoI GCNNNNNNNGC 2 cut(s) 308, 343
NciI CCSGG 2 cut(s) 81, 82
NdeII GATC 1 cut(s) 391
NlaIII CATG 2 cut(s) 78, 556
NlaIV GGNNCC 4 cut(s) 85, 277, 353, 393
NmeAIII GCCGAG 1 cut(s) 26
PaeR7I CTCGAG 1 cut(s) 356
PceI AGGCCT 1 cut(s) 578
PcsI WCGNNNNNNNCGW 2 cut(s) 534, 543
PdmI GAANNNNTTC 1 cut(s) 246
PfeI GAWTC 2 cut(s) 206, 247
PflFI GACNNNGTC 1 cut(s) 419
PkrI GCNGC 6 cut(s) 6, 9, 310, 339, 342, 345
PleI GAGTC 2 cut(s) 96, 509
PpsI GAGTC 2 cut(s) 96, 509
Ppu21I YACGTR 1 cut(s) 322
PsiI TTATAA 1 cut(s) 600
PspN4I GGNNCC 4 cut(s) 85, 277, 353, 393
PspPI GGNCC 2 cut(s) 84, 352
PsuI RGATCY 1 cut(s) 391
PsyI GACNNNGTC 1 cut(s) 419
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
RseI CAYNNNNRTG 1 cut(s) 126
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 6 cut(s) 5, 8, 309, 338, 341, 344
Sau3AI GATC 1 cut(s) 391
Sau96I GGNCC 2 cut(s) 84, 352
SchI GAGTC 2 cut(s) 96, 509
ScrFI CCNGG 2 cut(s) 81, 82
SduI GDGCHC 2 cut(s) 135, 278
Sfr274I CTCGAG 1 cut(s) 356
SlaI CTCGAG 1 cut(s) 356
SmaI CCCGGG 1 cut(s) 82
SmiMI CAYNNNNRTG 1 cut(s) 126
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
SnaBI TACGTA 1 cut(s) 322
SpeI ACTAGT 1 cut(s) 476
Sse9I AATT 4 cut(s) 51, 464, 484, 496
SseBI AGGCCT 1 cut(s) 578
SsiI CCGC 7 cut(s) 5, 150, 212, 253, 279, 302, 308
SspMI CTAG 3 cut(s) 36, 42, 477
StuI AGGCCT 1 cut(s) 578
StyD4I CCNGG 2 cut(s) 79, 80
TaaI ACNGT 1 cut(s) 292
TaiI ACGT 4 cut(s) 324, 378, 423, 531
TaqI TCGA 2 cut(s) 357, 537
TaqII GACCGA 1 cut(s) 527
TasI AATT 4 cut(s) 51, 464, 484, 496
TauI GCSGC 2 cut(s) 7, 311
TfiI GAWTC 2 cut(s) 206, 247
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TseI GCWGC 4 cut(s) 7, 337, 340, 343
TspDTI ATGAA 2 cut(s) 255, 523
TspGWI ACGGA 1 cut(s) 182
TspMI CCCGGG 1 cut(s) 80
Tth111I GACNNNGTC 1 cut(s) 419
XcmI CCANNNNNNNNNTGG 1 cut(s) 434
XhoI CTCGAG 1 cut(s) 356
XmaI CCCGGG 1 cut(s) 80
XmiI GTMKAC 1 cut(s) 144
XmnI GAANNNNTTC 1 cut(s) 246
XspI CTAG 3 cut(s) 36, 42, 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.