AT3G03310

PGAP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005852
Physical Location & Seq
Reverse (-)
778568 .. 781703
3136 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G03310.1

Sequence Viewer

Length: 1344 bp
ATGGGCTGGATTCCGTGTCCGTGCTGGGGAACCAACGACGATGAAAACGCCGGCGAGGTGGCGGATCGTGATCCGGTGCTTCTAGTATCTGGAATTGGAGGCTCTATTCTGCATTCTAAGAAGAAGAATTCAAAGTCTGAAATTCGGGTTTGGGTCCGAATATTTCTAGCTAACCTTGCCTTTAAGCAGAGCCTCTGGTCTCTCTATAATCCCAAAACTGGTTATACAGAGCCGTTGGATGATAATATTGAAGTATTGGTCCCTGATGATGACCATGGACTCTATGCAATTGACATTCTAGATCCCTCTTGGTTTGTAAAGCTTTGTCACTTGACGGAGGTTTATCACTTTCACGATATGATAGAAATGCTGGTTGGATGCGGTTATAAGAAGGGGACTACATTATTCGGTTATGGTTACGATTTCCGTCAAAGCAATAGGATCGATCTACTTATACTAGGTCTGAAGAAGAAGCTGGAAACTGCATATAAACGTTCAGGGGGGAGAAAAGTCACTATCATCTCCCATTCAATGGGAGGACTTATGGTTTCATGTTTCATGTATCTCCATCCGGAGGCATTTTCCAAGTATGTAAATAAATGGATTACAATTGCAACACCTTTCCAAGGAGCACCAGGGTGCATCAATGATTCAATCTTGACTGGAGTGCAATTTGTGGAAGGGTTAGAAAGTTTCTTTTTTGTGTCACGTTGGACGATGCACCAACTGTTGGTCGAATGCCCATCTATATATGAGATGATGGCAAATCCAGACTTTAAGTGGAAAAAGCAACCAGAGATTCGAGTTTGGCGTAAGAAATCTGAAAACGACGTTGATACTTCTGTAGAACTGGAATCATTTGGCTTAATCGAGAGTATTGATCTATTCAACGATGCATTAAAAAATAACGAGCTAAGCTATGGTGGGAATAAAATAGCTTTGCCCTTTAACTTTGCTATCCTCGACTGGGCTGCTAAGACAAGAGAAATTCTCAACAAAGCGCAACTTCCTGATGGAGTGTCCTTCTATAACATATATGGAGTGTCACTTAATACACCCTTTGATGTTTGTTATGGCACAGAGACTTCTCCGATAGACGATTTGTCTGAAATATGTCAAACTATGCCTGAGTATACATATGTAGATGGAGATGGAACTGTCCCTGCTGAATCAGCTGCAGCTGCTCAGTTTAAAGCAGTTGCTAGCGTAGGAGTTTCGGGTAGCCACCGCGGGCTTCTCCGTGATGAAAGAGTGTTTGAGCTCATTCAACAATGGTTAGGAGTTGAGCCCAAGAAGGCTAAACGGAAGCATTTAAGGACTCACAAAGTAGTTGATTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110
Pfam Domains
Protein Families
LCAT

Protein Analysis

447

Amino Acids

50.35

Weight (kDa)

5.72

Isoelectric Point (pI)

41.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 68 - 393 8.8e-26 Lecithin:cholesterol acyltransferase
PGAP1 PF07819 163 - 220 6.5e-07 PGAP1-like alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 387
AccB7I CCANNNNNTGG 2 cut(s) 532, 730
AccI GTMKAC 1 cut(s) 1133
AccII CGCG 1 cut(s) 1230
AccIII TCCGGA 1 cut(s) 571
AciI CCGC 4 cut(s) 62, 381, 1228, 1230
AclI AACGTT 1 cut(s) 493
AclWI GGATC 4 cut(s) 65, 72, 296, 449
AcsI RAATTY 3 cut(s) 127, 141, 987
AcuI CTGAAG 1 cut(s) 485
AfiI CCNNNNNNNGG 7 cut(s) 26, 218, 532, 574, 626, 730, 967
AgsI TTSAA 6 cut(s) 132, 251, 531, 654, 889, 1268
AhdI GACNNNNNGTC 1 cut(s) 1102
AjnI CCWGG 1 cut(s) 634
AjuI GAANNNNNNNTTGG 2 cut(s) 357, 389
AleI CACNNNNGTG 1 cut(s) 637
AluBI AGCT 9 cut(s) 170, 322, 475, 913, 918, 938, 1175, 1181, 1261
AluI AGCT 9 cut(s) 170, 322, 475, 913, 918, 938, 1175, 1181, 1261
Alw21I GWGCWC 2 cut(s) 634, 1263
Alw26I GTCTC 2 cut(s) 204, 1076
AlwI GGATC 4 cut(s) 65, 72, 296, 449
Aor13HI TCCGGA 1 cut(s) 571
ApeKI GCWGC 4 cut(s) 971, 1175, 1178, 1181
ApoI RAATTY 3 cut(s) 127, 141, 987
AspLEI GCGC 1 cut(s) 1003
AspS9I GGNCC 2 cut(s) 154, 259
AsuNHI GCTAGC 1 cut(s) 1202
AvaII GGWCC 2 cut(s) 154, 259
BaeI ACNNNNGTAYC 2 cut(s) 828, 861
BanII GRGCYC 2 cut(s) 1263, 1290
Bbv12I GWGCWC 2 cut(s) 634, 1263
BbvI GCAGC 4 cut(s) 958, 1162, 1168, 1190
BccI CCATC 6 cut(s) 576, 751, 754, 1007, 1139, 1145
BceAI ACGGC 1 cut(s) 217
BcgI CGANNNNNNTGC 4 cut(s) 424, 458, 953, 987
BciT130I CCWGG 1 cut(s) 636
BcoDI GTCTC 2 cut(s) 204, 1076
BfaI CTAG 5 cut(s) 83, 167, 299, 458, 1203
BfmI CTRYAG 2 cut(s) 843, 1176
BisI GCNGC 4 cut(s) 972, 1176, 1179, 1182
BlpI GCTNAGC 1 cut(s) 914
BlsI GCNGC 4 cut(s) 973, 1177, 1180, 1183
Bme1390I CCNGG 1 cut(s) 636
Bme18I GGWCC 2 cut(s) 154, 259
BmeRI GACNNNNNGTC 1 cut(s) 1102
BmgT120I GGNCC 2 cut(s) 154, 259
BmiI GGNNCC 3 cut(s) 31, 155, 261
BmrFI CCNGG 1 cut(s) 636
BmrI ACTGGG 1 cut(s) 976
BmsI GCATC 4 cut(s) 368, 651, 708, 883
BmtI GCTAGC 1 cut(s) 1206
BmuI ACTGGG 1 cut(s) 976
BplI GAGNNNNNCTC 2 cut(s) 975, 1007
BpmI CTGGAG 1 cut(s) 684
Bpu1102I GCTNAGC 1 cut(s) 914
Bsa29I ATCGAT 1 cut(s) 444
BsaBI GATNNNNATC 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 4 cut(s) 274, 625, 635, 1228
BsaWI WCCGGW 2 cut(s) 73, 571
Bsc4I CCNNNNNNNGG 7 cut(s) 26, 218, 532, 574, 626, 730, 967
Bse118I RCCGGY 1 cut(s) 50
Bse1I ACTGG 4 cut(s) 223, 667, 855, 971
Bse8I GATNNNNATC 1 cut(s) 69
BseAI TCCGGA 1 cut(s) 571
BseBI CCWGG 1 cut(s) 636
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 4 cut(s) 274, 625, 635, 1228
BseGI GGATG 3 cut(s) 244, 383, 568
BseJI GATNNNNATC 1 cut(s) 69
BseLI CCNNNNNNNGG 7 cut(s) 26, 218, 532, 574, 626, 730, 967
BseMII CTCAG 2 cut(s) 1119, 1199
BseNI ACTGG 4 cut(s) 223, 667, 855, 971
BseXI GCAGC 4 cut(s) 958, 1162, 1168, 1190
BseYI CCCAGC 1 cut(s) 24
Bsh1236I CGCG 1 cut(s) 1230
BshVI ATCGAT 1 cut(s) 444
BsiHKAI GWGCWC 2 cut(s) 634, 1263
BsiSI CCGG 3 cut(s) 51, 74, 572
BslFI GGGAC 3 cut(s) 245, 409, 1145
BslI CCNNNNNNNGG 7 cut(s) 26, 218, 532, 574, 626, 730, 967
BsmAI GTCTC 2 cut(s) 204, 1076
BsmFI GGGAC 3 cut(s) 245, 409, 1145
BsmI GAATGC 2 cut(s) 112, 743
Bso31I GGTCTC 1 cut(s) 204
Bsp1286I GDGCHC 3 cut(s) 634, 1263, 1290
Bsp13I TCCGGA 1 cut(s) 571
Bsp143I GATC 6 cut(s) 64, 70, 301, 441, 445, 880
Bsp1720I GCTNAGC 1 cut(s) 914
Bsp19I CCATGG 1 cut(s) 274
BspACI CCGC 4 cut(s) 62, 381, 1228, 1230
BspCNI CTCAG 2 cut(s) 1120, 1198
BspDI ATCGAT 1 cut(s) 444
BspEI TCCGGA 1 cut(s) 571
BspFNI CGCG 1 cut(s) 1230
BspLI GGNNCC 3 cut(s) 31, 155, 261
BspMAI CTGCAG 1 cut(s) 1180
BspOI GCTAGC 1 cut(s) 1206
BspPI GGATC 4 cut(s) 65, 72, 296, 449
BspTNI GGTCTC 1 cut(s) 204
BsrFI RCCGGY 1 cut(s) 50
BsrI ACTGG 4 cut(s) 223, 667, 855, 971
BssAI RCCGGY 1 cut(s) 50
BssECI CCNNGG 4 cut(s) 274, 625, 635, 1228
BssMI GATC 6 cut(s) 64, 70, 301, 441, 445, 880
BssNAI GTATAC 1 cut(s) 1134
BssT1I CCWWGG 2 cut(s) 274, 625
Bst1107I GTATAC 1 cut(s) 1134
Bst2UI CCWGG 1 cut(s) 636
Bst4CI ACNGT 2 cut(s) 729, 1159
BstC8I GCNNGC 3 cut(s) 52, 1204, 1232
BstDEI CTNAG 5 cut(s) 117, 914, 975, 1128, 1185
BstDSI CCRYGG 2 cut(s) 274, 1228
BstENI CCTNNNNNAGG 1 cut(s) 624
BstF5I GGATG 3 cut(s) 244, 383, 568
BstFNI CGCG 1 cut(s) 1230
BstHHI GCGC 1 cut(s) 1003
BstKTI GATC 6 cut(s) 67, 73, 304, 444, 448, 883
BstMAI GTCTC 2 cut(s) 204, 1076
BstMBI GATC 6 cut(s) 64, 70, 301, 441, 445, 880
BstMWI GCNNNNNNNGC 3 cut(s) 176, 1172, 1181
BstNI CCWGG 1 cut(s) 636
BstSCI CCNGG 1 cut(s) 634
BstSFI CTRYAG 2 cut(s) 843, 1176
BstUI CGCG 1 cut(s) 1230
BstV1I GCAGC 4 cut(s) 958, 1162, 1168, 1190
BstX2I RGATCY 1 cut(s) 301
BstYI RGATCY 1 cut(s) 301
BstZ17I GTATAC 1 cut(s) 1134
Bsu15I ATCGAT 1 cut(s) 444
BsuTUI ATCGAT 1 cut(s) 444
BtgI CCRYGG 2 cut(s) 274, 1228
BtsCI GGATG 3 cut(s) 244, 383, 568
Cac8I GCNNGC 3 cut(s) 52, 1204, 1232
CfoI GCGC 1 cut(s) 1003
Cfr10I RCCGGY 1 cut(s) 50
Cfr13I GGNCC 2 cut(s) 154, 259
Cfr42I CCGCGG 1 cut(s) 1231
ClaI ATCGAT 1 cut(s) 444
CviAII CATG 3 cut(s) 275, 552, 559
DdeI CTNAG 5 cut(s) 117, 914, 975, 1128, 1185
DpnI GATC 6 cut(s) 66, 72, 303, 443, 447, 882
DpnII GATC 6 cut(s) 64, 70, 301, 441, 445, 880
DraI TTTAAA 1 cut(s) 1192
DriI GACNNNNNGTC 1 cut(s) 1102
Eam1105I GACNNNNNGTC 1 cut(s) 1102
EciI GGCGGA 1 cut(s) 77
Ecl136II GAGCTC 1 cut(s) 1261
Eco130I CCWWGG 2 cut(s) 274, 625
Eco24I GRGCYC 2 cut(s) 1263, 1290
Eco31I GGTCTC 1 cut(s) 204
Eco47I GGWCC 2 cut(s) 154, 259
Eco53kI GAGCTC 1 cut(s) 1261
Eco57I CTGAAG 1 cut(s) 485
EcoICRI GAGCTC 1 cut(s) 1261
EcoNI CCTNNNNNAGG 1 cut(s) 624
EcoRI GAATTC 1 cut(s) 127
EcoRII CCWGG 1 cut(s) 634
EcoT14I CCWWGG 2 cut(s) 274, 625
EcoT22I ATGCAT 1 cut(s) 898
EcoT38I GRGCYC 2 cut(s) 1263, 1290
ErhI CCWWGG 2 cut(s) 274, 625
FaeI CATG 3 cut(s) 278, 555, 562
FalI AAGNNNNNCTT 2 cut(s) 990, 1022
FaqI GGGAC 3 cut(s) 245, 409, 1145
FatI CATG 3 cut(s) 274, 551, 558
FauI CCCGC 1 cut(s) 1223
FauNDI CATATG 1 cut(s) 1138
FblI GTMKAC 1 cut(s) 1133
Fnu4HI GCNGC 4 cut(s) 972, 1176, 1179, 1182
FokI GGATG 3 cut(s) 251, 390, 555
FriOI GRGCYC 2 cut(s) 1263, 1290
Fsp4HI GCNGC 4 cut(s) 972, 1176, 1179, 1182
FspBI CTAG 5 cut(s) 83, 167, 299, 458, 1203
GlaI GCGC 1 cut(s) 1002
GluI GCNGC 4 cut(s) 972, 1176, 1179, 1182
GsaI CCCAGC 1 cut(s) 28
GsuI CTGGAG 1 cut(s) 684
HapII CCGG 3 cut(s) 51, 74, 572
HhaI GCGC 1 cut(s) 1003
Hin1II CATG 3 cut(s) 278, 555, 562
Hin6I GCGC 1 cut(s) 1001
HinP1I GCGC 1 cut(s) 1001
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 8 cut(s) 10, 279, 650, 799, 854, 1169, 1318, 1334
HpaII CCGG 3 cut(s) 51, 74, 572
Hpy166II GTNNAC 1 cut(s) 1134
Hpy188I TCNGA 6 cut(s) 139, 158, 465, 823, 1092, 1108
Hpy188III TCNNGA 9 cut(s) 68, 90, 299, 353, 572, 658, 770, 871, 1010
Hpy8I GTNNAC 1 cut(s) 1134
Hpy99I CGWCG 2 cut(s) 41, 833
HpyAV CCTTC 4 cut(s) 385, 674, 1033, 1288
HpyCH4III ACNGT 2 cut(s) 729, 1159
HpyCH4IV ACGT 3 cut(s) 493, 709, 831
HpyCH4V TGCA 9 cut(s) 112, 287, 485, 614, 642, 670, 721, 896, 1178
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 1172, 1181
HpyF3I CTNAG 5 cut(s) 117, 914, 975, 1128, 1185
HpySE526I ACGT 3 cut(s) 493, 709, 831
Hsp92II CATG 3 cut(s) 278, 555, 562
HspAI GCGC 1 cut(s) 1001
Kpn2I TCCGGA 1 cut(s) 571
KroI GCCGGC 1 cut(s) 50
KroNI GCCGGC 1 cut(s) 52
KspI CCGCGG 1 cut(s) 1231
Kzo9I GATC 6 cut(s) 64, 70, 301, 441, 445, 880
LmnI GCTCC 1 cut(s) 629
Lsp1109I GCAGC 4 cut(s) 958, 1162, 1168, 1190
LweI GCATC 4 cut(s) 368, 651, 708, 883
MaeI CTAG 5 cut(s) 83, 167, 299, 458, 1203
MaeII ACGT 3 cut(s) 493, 709, 831
MaeIII GTNAC 5 cut(s) 326, 416, 511, 705, 1044
MalI GATC 6 cut(s) 66, 72, 303, 443, 447, 882
MboI GATC 6 cut(s) 64, 70, 301, 441, 445, 880
MboII GAAGA 4 cut(s) 133, 136, 478, 481
MfeI CAATTG 2 cut(s) 288, 609
MflI RGATCY 1 cut(s) 301
MhlI GDGCHC 3 cut(s) 634, 1263, 1290
MluCI AATT 7 cut(s) 93, 127, 141, 288, 609, 671, 987
MlyI GAGTC 2 cut(s) 273, 1312
MmeI TCCRAC 3 cut(s) 216, 355, 692
MnlI CCTC 8 cut(s) 49, 92, 203, 316, 331, 530, 568, 971
Mph1103I ATGCAT 1 cut(s) 898
MreI CGCCGGCG 1 cut(s) 50
MroI TCCGGA 1 cut(s) 571
MroNI GCCGGC 1 cut(s) 50
MseI TTAA 9 cut(s) 183, 777, 866, 899, 948, 1050, 1191, 1313, 1342
MslI CAYNNNNRTG 1 cut(s) 637
MspA1I CMGCKG 3 cut(s) 1175, 1181, 1230
MspI CCGG 3 cut(s) 51, 74, 572
MspR9I CCNGG 1 cut(s) 636
MunI CAATTG 2 cut(s) 288, 609
Mva1269I GAATGC 2 cut(s) 112, 743
MvaI CCWGG 1 cut(s) 636
MvnI CGCG 1 cut(s) 1230
MwoI GCNNNNNNNGC 3 cut(s) 176, 1172, 1181
NaeI GCCGGC 1 cut(s) 52
NcoI CCATGG 1 cut(s) 274
NdeI CATATG 1 cut(s) 1138
NdeII GATC 6 cut(s) 64, 70, 301, 441, 445, 880
NgoMIV GCCGGC 1 cut(s) 50
NheI GCTAGC 1 cut(s) 1202
NlaIII CATG 3 cut(s) 278, 555, 562
NlaIV GGNNCC 3 cut(s) 31, 155, 261
NmuCI GTSAC 4 cut(s) 326, 511, 705, 1044
NsiI ATGCAT 1 cut(s) 898
OliI CACNNNNGTG 1 cut(s) 637
PcsI WCGNNNNNNNCGW 1 cut(s) 808
PctI GAATGC 2 cut(s) 112, 743
PdiI GCCGGC 1 cut(s) 52
PfeI GAWTC 6 cut(s) 10, 650, 799, 854, 1169, 1334
PflMI CCANNNNNTGG 2 cut(s) 532, 730
PkrI GCNGC 4 cut(s) 973, 1177, 1180, 1183
PleI GAGTC 2 cut(s) 273, 1312
PpsI GAGTC 2 cut(s) 273, 1312
PsiI TTATAA 1 cut(s) 387
Psp124BI GAGCTC 1 cut(s) 1263
Psp1406I AACGTT 1 cut(s) 493
Psp6I CCWGG 1 cut(s) 634
PspFI CCCAGC 1 cut(s) 24
PspGI CCWGG 1 cut(s) 634
PspN4I GGNNCC 3 cut(s) 31, 155, 261
PspPI GGNCC 2 cut(s) 154, 259
PstI CTGCAG 1 cut(s) 1180
PsuI RGATCY 1 cut(s) 301
PvuII CAGCTG 2 cut(s) 1175, 1181
RseI CAYNNNNRTG 1 cut(s) 637
SacI GAGCTC 1 cut(s) 1263
SacII CCGCGG 1 cut(s) 1231
SaqAI TTAA 9 cut(s) 183, 777, 866, 899, 948, 1050, 1191, 1313, 1342
SatI GCNGC 4 cut(s) 972, 1176, 1179, 1182
Sau3AI GATC 6 cut(s) 64, 70, 301, 441, 445, 880
Sau96I GGNCC 2 cut(s) 154, 259
SchI GAGTC 2 cut(s) 273, 1312
ScrFI CCNGG 1 cut(s) 636
SduI GDGCHC 3 cut(s) 634, 1263, 1290
SfaNI GCATC 4 cut(s) 368, 651, 708, 883
SfcI CTRYAG 2 cut(s) 843, 1176
Sfr303I CCGCGG 1 cut(s) 1231
SgrAI CRCCGGYG 1 cut(s) 50
SgrBI CCGCGG 1 cut(s) 1231
SinI GGWCC 2 cut(s) 154, 259
SmiMI CAYNNNNRTG 1 cut(s) 637
Sse9I AATT 7 cut(s) 93, 127, 141, 288, 609, 671, 987
SsiI CCGC 4 cut(s) 62, 381, 1228, 1230
SspI AATATT 2 cut(s) 162, 247
SspMI CTAG 5 cut(s) 83, 167, 299, 458, 1203
SstI GAGCTC 1 cut(s) 1263
StyD4I CCNGG 1 cut(s) 634
StyI CCWWGG 2 cut(s) 274, 625
TaaI ACNGT 2 cut(s) 729, 1159
TaiI ACGT 3 cut(s) 496, 712, 834
TaqI TCGA 5 cut(s) 444, 735, 802, 870, 963
TasI AATT 7 cut(s) 93, 127, 141, 288, 609, 671, 987
TfiI GAWTC 6 cut(s) 10, 650, 799, 854, 1169, 1334
Tru1I TTAA 9 cut(s) 183, 777, 866, 899, 948, 1050, 1191, 1313, 1342
Tru9I TTAA 9 cut(s) 183, 777, 866, 899, 948, 1050, 1191, 1313, 1342
TseFI GTSAC 4 cut(s) 326, 511, 705, 1044
TseI GCWGC 4 cut(s) 971, 1175, 1178, 1181
Tsp45I GTSAC 4 cut(s) 326, 511, 705, 1044
TspDTI ATGAA 4 cut(s) 57, 540, 547, 1260
TspGWI ACGGA 5 cut(s) 9, 350, 416, 1229, 1318
Van91I CCANNNNNTGG 2 cut(s) 532, 730
VpaK11BI GGWCC 2 cut(s) 154, 259
XagI CCTNNNNNAGG 1 cut(s) 624
XapI RAATTY 3 cut(s) 127, 141, 987
XbaI TCTAGA 1 cut(s) 298
XcmI CCANNNNNNNNNTGG 1 cut(s) 777
XmiI GTMKAC 1 cut(s) 1133
XspI CTAG 5 cut(s) 83, 167, 299, 458, 1203
Zsp2I ATGCAT 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.