Rroxscaffold_6G00425760

Phospholipase A(1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005852
Physical Location & Seq
Reverse (-)
46759110 .. 46766115
7006 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00425760.1

Sequence Viewer

Length: 1509 bp
ATGGTGTTGGATATGCCGAAGCAAATTCACGAGATCAAGGATTTCCTCCTTACTGCAAGAAGGAAGGATGCGCGTTCTGTCAAAATCAAGAGAACAAAGGACGCTGTGAAGTTCAAGGTTCGATGCTCAAAGTACCTCTACACCCTTTGCGTGTTTGATTCTGAGAAGGCAGACAAGTTGAAGCAATCTCTTCCTCCAGGTTATACAGAAACTTTAGACAAAGACAATGAAATTGTTGTCCCAGATGATGATTATGGCCTCTATGCAATTGATATTCTAGACCCGTCCCCGTTTGTGAAATGCATACATTTGAAAGAGGTATACTATTTTCATGATATGATAAATATGCTTGTGGGCTGTGGCTATAAGAAGGGAACCACATTGTTTGGATATGGCTATGATTTCCGACAAAGCAACAGGTATTTAACTACCACTATGTCTGACTACCATGGCGATTATGTTGGCAGAATTGAAAAGTTAATGGAAGATCTTAAAGTGAAATTGGAAACTGCTTACAAGGCTTCTGGGGGTAGAAAAGTAAATATAATTTCCCATTCAATGGGTGGACTGCTAGTCATGTGTTTTATGTCACTTCATCATGAGGCCTTTTCAAAGTATGTGAGTAAATGGATTTGCATCGGTTGCCCTTTTCAAGGTGCACCAGGATGCATCAATGATTCTCTTTTAACTGGACTGCAGTTTGTTGAAGGCTTGGAAAGCTTTTTTTGTATCAAGGTGGACTATGCACCAGCTGGTAATACATTAAGCTTAATTGGCTTCAGTTTATCATCTTTCTTGTTGGTCGAGTGTCCATCAATCTACGAGATGTTGGCCAACCCAGATTTTAATTGGAAAAAGCAGCCGGAAATTCAGGTTTGGAGGAAGCATTCTAAGGATGGGGAAACTTCTATTGAACTGGAATCTTATGGTCCCATTGAAAGTATTACTTTGTTTAGAGAAGCATTGAGACATAATGAGCTAACTTATGATGGTAAAACTGTAGCTTTGCCATTTAACTTTGCTATTTCCAGATGGGCTGCCGAGACTCGCCAAGTTCTCAACAATGCCAAACTACCAGATGGAGTCTGCTTCTATAACATTTATGGAATATCCTTTGACACACCTTTTGATGTTTGTTATGGCTCGAAGGCATCTCCAATTGAAGACTTGTCTGAAATATGCCATTCAATGCCTCAGTATTCTTATGTGGATGGAGATGAAACAGTTTCTGCTGAATCAGCAAAGGTGTTGTATCTGACTTTTCATATTATGTTGCAGACTTGTATTCTTAGTTTCTACTTTCTAGCCAGTGTTAAGCCATACACTGGCTATGACAGCTTTATTTATGGATTTGCTGCAGTTGAAAGAGTAGCAATCGCTGCAAGACACCGGGAACTATTAAGTGACAAAACGGTTTTTCAACATATCAAGAAATGGTTGGGAGTTGAGCAGAAGGTCCACATACATTCCAAGACATCCAGAGTTTCAGATAAGCCTGTGAGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110
Pfam Domains
Protein Families
LCAT

Protein Analysis

502

Amino Acids

57.2

Weight (kDa)

6.74

Isoelectric Point (pI)

44.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L38e PF01781 6 - 69 6.2e-32 Ribosomal L38e protein family
LCAT PF02450 84 - 225 3.2e-19 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 559, 1325
AccI GTMKAC 1 cut(s) 321
AccII CGCG 1 cut(s) 73
AcoI YGGCCR 1 cut(s) 831
AcsI RAATTY 2 cut(s) 24, 867
AcuI CTGAAG 1 cut(s) 763
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 3 cut(s) 559, 653, 1325
AhdI GACNNNNNGTC 1 cut(s) 572
AjnI CCWGG 2 cut(s) 196, 661
AluBI AGCT 6 cut(s) 720, 752, 768, 979, 1004, 1338
AluI AGCT 6 cut(s) 720, 752, 768, 979, 1004, 1338
Alw21I GWGCWC 1 cut(s) 661
Alw26I GTCTC 2 cut(s) 961, 1037
Alw44I GTGCAC 1 cut(s) 657
AlwNI CAGNNNCTG 1 cut(s) 1229
AoxI GGCC 3 cut(s) 256, 603, 831
ApaLI GTGCAC 1 cut(s) 657
ApeKI GCWGC 4 cut(s) 859, 1037, 1355, 1379
ApoI RAATTY 2 cut(s) 24, 867
ArsI GACNNNNNNTTYG 2 cut(s) 1109, 1141
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 2 cut(s) 929, 1456
AsuC2I CCSGG 1 cut(s) 1391
AvaII GGWCC 2 cut(s) 929, 1456
BaeGI GKGCMC 1 cut(s) 661
BalI TGGCCA 1 cut(s) 833
BauI CACGAG 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 1170
Bbv12I GWGCWC 1 cut(s) 661
BbvI GCAGC 4 cut(s) 871, 1024, 1342, 1366
BccI CCATC 6 cut(s) 820, 890, 983, 1026, 1073, 1205
BciT130I CCWGG 2 cut(s) 198, 663
BcnI CCSGG 1 cut(s) 1391
BcoDI GTCTC 2 cut(s) 961, 1037
BfaI CTAG 3 cut(s) 278, 572, 1304
BfmI CTRYAG 3 cut(s) 695, 999, 1356
BglII AGATCT 1 cut(s) 487
BisI GCNGC 4 cut(s) 860, 1038, 1356, 1380
BlsI GCNGC 4 cut(s) 861, 1039, 1357, 1381
Bme1390I CCNGG 3 cut(s) 198, 663, 1391
Bme18I GGWCC 2 cut(s) 929, 1456
BmeRI GACNNNNNGTC 1 cut(s) 572
BmgT120I GGNCC 2 cut(s) 929, 1456
BmiI GGNNCC 2 cut(s) 376, 931
BmrFI CCNGG 3 cut(s) 198, 663, 1391
BmsI GCATC 6 cut(s) 58, 113, 645, 656, 678, 1160
BpiI GAAGAC 1 cut(s) 1170
BpmI CTGGAG 1 cut(s) 180
BpuMI CCSGG 1 cut(s) 1391
BsaBI GATNNNNATC 1 cut(s) 635
BsaJI CCNNGG 1 cut(s) 448
Bsc4I CCNNNNNNNGG 3 cut(s) 559, 653, 1325
Bse1I ACTGG 4 cut(s) 694, 921, 1308, 1330
Bse8I GATNNNNATC 1 cut(s) 635
BseBI CCWGG 2 cut(s) 198, 663
BseDI CCNNGG 1 cut(s) 448
BseGI GGATG 5 cut(s) 73, 671, 901, 1216, 1475
BseJI GATNNNNATC 1 cut(s) 635
BseLI CCNNNNNNNGG 3 cut(s) 559, 653, 1325
BseMII CTCAG 2 cut(s) 153, 1208
BseNI ACTGG 4 cut(s) 694, 921, 1308, 1330
BseSI GKGCMC 1 cut(s) 661
BseXI GCAGC 4 cut(s) 871, 1024, 1342, 1366
Bsh1236I CGCG 1 cut(s) 73
BshFI GGCC 3 cut(s) 258, 605, 833
BsiHKAI GWGCWC 1 cut(s) 661
BsiSI CCGG 2 cut(s) 863, 1390
BslFI GGGAC 3 cut(s) 224, 271, 915
BslI CCNNNNNNNGG 3 cut(s) 559, 653, 1325
BsmAI GTCTC 2 cut(s) 961, 1037
BsmFI GGGAC 3 cut(s) 224, 271, 915
BsmI GAATGC 1 cut(s) 886
BsnI GGCC 3 cut(s) 258, 605, 833
Bsp1286I GDGCHC 1 cut(s) 661
Bsp143I GATC 2 cut(s) 33, 487
Bsp19I CCATGG 1 cut(s) 448
BspANI GGCC 3 cut(s) 258, 605, 833
BspCNI CTCAG 2 cut(s) 154, 1207
BspFNI CGCG 1 cut(s) 73
BspHI TCATGA 2 cut(s) 331, 598
BspLI GGNNCC 2 cut(s) 376, 931
BspMAI CTGCAG 2 cut(s) 699, 1360
BsrI ACTGG 4 cut(s) 694, 921, 1308, 1330
BssECI CCNNGG 1 cut(s) 448
BssMI GATC 2 cut(s) 33, 487
BssNAI GTATAC 1 cut(s) 322
BssSI CACGAG 1 cut(s) 29
BssT1I CCWWGG 1 cut(s) 448
Bst1107I GTATAC 1 cut(s) 322
Bst2BI CACGAG 1 cut(s) 29
Bst2UI CCWGG 2 cut(s) 198, 663
Bst4CI ACNGT 3 cut(s) 1000, 1225, 1414
Bst6I CTCTTC 1 cut(s) 195
BstAPI GCANNNNNTGC 2 cut(s) 642, 1379
BstDEI CTNAG 4 cut(s) 162, 891, 1194, 1289
BstDSI CCRYGG 1 cut(s) 448
BstENI CCTNNNNNAGG 1 cut(s) 651
BstF5I GGATG 5 cut(s) 73, 671, 901, 1216, 1475
BstFNI CGCG 1 cut(s) 73
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 2 cut(s) 36, 490
BstMAI GTCTC 2 cut(s) 961, 1037
BstMBI GATC 2 cut(s) 33, 487
BstMWI GCNNNNNNNGC 7 cut(s) 518, 642, 717, 774, 1238, 1335, 1379
BstNI CCWGG 2 cut(s) 198, 663
BstSCI CCNGG 3 cut(s) 196, 661, 1389
BstSFI CTRYAG 3 cut(s) 695, 999, 1356
BstSLI GKGCMC 1 cut(s) 661
BstUI CGCG 1 cut(s) 73
BstV1I GCAGC 4 cut(s) 871, 1024, 1342, 1366
BstV2I GAAGAC 1 cut(s) 1170
BstX2I RGATCY 1 cut(s) 487
BstYI RGATCY 1 cut(s) 487
BstZ17I GTATAC 1 cut(s) 322
BsuRI GGCC 3 cut(s) 258, 605, 833
BtgI CCRYGG 1 cut(s) 448
BtsCI GGATG 5 cut(s) 73, 671, 901, 1216, 1475
BtsIMutI CAGTG 2 cut(s) 1315, 1323
CaiI CAGNNNCTG 1 cut(s) 1229
CciI TCATGA 2 cut(s) 331, 598
CfoI GCGC 1 cut(s) 73
Cfr13I GGNCC 2 cut(s) 929, 1456
CseI GACGC 1 cut(s) 110
Csp6I GTAC 1 cut(s) 133
CspCI CAANNNNNGTGG 2 cut(s) 367, 402
CviAII CATG 4 cut(s) 332, 449, 577, 599
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 4 cut(s) 162, 891, 1194, 1289
DpnI GATC 2 cut(s) 35, 489
DpnII GATC 2 cut(s) 33, 487
DriI GACNNNNNGTC 1 cut(s) 572
EaeI YGGCCR 1 cut(s) 831
Eam1104I CTCTTC 1 cut(s) 195
Eam1105I GACNNNNNGTC 1 cut(s) 572
EarI CTCTTC 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 448
Eco147I AGGCCT 1 cut(s) 605
Eco47I GGWCC 2 cut(s) 929, 1456
Eco57I CTGAAG 1 cut(s) 763
EcoNI CCTNNNNNAGG 1 cut(s) 651
EcoRII CCWGG 2 cut(s) 196, 661
EcoT14I CCWWGG 1 cut(s) 448
EcoT22I ATGCAT 2 cut(s) 305, 671
ErhI CCWWGG 1 cut(s) 448
FaeI CATG 4 cut(s) 335, 452, 580, 602
FalI AAGNNNNNCTT 2 cut(s) 931, 963
FaqI GGGAC 3 cut(s) 224, 271, 915
FatI CATG 4 cut(s) 331, 448, 576, 598
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 4 cut(s) 860, 1038, 1356, 1380
FokI GGATG 5 cut(s) 80, 678, 908, 1223, 1462
Fsp4HI GCNGC 4 cut(s) 860, 1038, 1356, 1380
FspBI CTAG 3 cut(s) 278, 572, 1304
GlaI GCGC 1 cut(s) 72
GluI GCNGC 4 cut(s) 860, 1038, 1356, 1380
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 3 cut(s) 258, 605, 833
HapII CCGG 2 cut(s) 863, 1390
HgaI GACGC 1 cut(s) 110
HhaI GCGC 1 cut(s) 73
Hin1II CATG 4 cut(s) 335, 452, 580, 602
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HindIII AAGCTT 2 cut(s) 718, 766
HinfI GANTC 6 cut(s) 158, 677, 920, 1045, 1083, 1235
HpaII CCGG 2 cut(s) 863, 1390
Hpy166II GTNNAC 5 cut(s) 322, 566, 659, 739, 1459
Hpy188I TCNGA 6 cut(s) 163, 407, 442, 1174, 1257, 1489
Hpy188III TCNNGA 8 cut(s) 29, 88, 278, 332, 599, 1029, 1429, 1479
Hpy8I GTNNAC 5 cut(s) 322, 566, 659, 739, 1459
HpyAV CCTTC 7 cut(s) 54, 58, 160, 364, 701, 1141, 1447
HpyCH4III ACNGT 3 cut(s) 1000, 1225, 1414
HpyF10VI GCNNNNNNNGC 7 cut(s) 518, 642, 717, 774, 1238, 1335, 1379
HpyF3I CTNAG 4 cut(s) 162, 891, 1194, 1289
Hsp92II CATG 4 cut(s) 335, 452, 580, 602
HspAI GCGC 1 cut(s) 71
Kzo9I GATC 2 cut(s) 33, 487
Lsp1109I GCAGC 4 cut(s) 871, 1024, 1342, 1366
LweI GCATC 6 cut(s) 58, 113, 645, 656, 678, 1160
MaeI CTAG 3 cut(s) 278, 572, 1304
MaeIII GTNAC 2 cut(s) 588, 1403
MalI GATC 2 cut(s) 35, 489
MboI GATC 2 cut(s) 33, 487
MboII GAAGA 3 cut(s) 182, 497, 1175
MfeI CAATTG 2 cut(s) 267, 1158
MflI RGATCY 1 cut(s) 487
MhlI GDGCHC 1 cut(s) 661
MlsI TGGCCA 1 cut(s) 833
MluNI TGGCCA 1 cut(s) 833
MlyI GAGTC 2 cut(s) 1039, 1092
MmeI TCCRAC 1 cut(s) 430
MnlI CCTC 9 cut(s) 56, 146, 204, 269, 310, 595, 873, 1203, 1494
Mox20I TGGCCA 1 cut(s) 833
Mph1103I ATGCAT 2 cut(s) 305, 671
MscI TGGCCA 1 cut(s) 833
MslI CAYNNNNRTG 1 cut(s) 664
Msp20I TGGCCA 1 cut(s) 833
MspA1I CMGCKG 1 cut(s) 752
MspI CCGG 2 cut(s) 863, 1390
MspR9I CCNGG 3 cut(s) 198, 663, 1391
MunI CAATTG 2 cut(s) 267, 1158
Mva1269I GAATGC 1 cut(s) 886
MvaI CCWGG 2 cut(s) 198, 663
MvnI CGCG 1 cut(s) 73
MwoI GCNNNNNNNGC 7 cut(s) 518, 642, 717, 774, 1238, 1335, 1379
NciI CCSGG 1 cut(s) 1391
NcoI CCATGG 1 cut(s) 448
NdeII GATC 2 cut(s) 33, 487
NlaIII CATG 4 cut(s) 335, 452, 580, 602
NlaIV GGNNCC 2 cut(s) 376, 931
NmeAIII GCCGAG 1 cut(s) 1066
NmuCI GTSAC 2 cut(s) 588, 1403
NsiI ATGCAT 2 cut(s) 305, 671
PagI TCATGA 2 cut(s) 331, 598
PceI AGGCCT 1 cut(s) 605
PctI GAATGC 1 cut(s) 886
PfeI GAWTC 4 cut(s) 158, 677, 920, 1235
PflMI CCANNNNNTGG 2 cut(s) 559, 1325
PkrI GCNGC 4 cut(s) 861, 1039, 1357, 1381
PleI GAGTC 2 cut(s) 1039, 1091
PpsI GAGTC 2 cut(s) 1039, 1091
Psp6I CCWGG 2 cut(s) 196, 661
PspGI CCWGG 2 cut(s) 196, 661
PspN4I GGNNCC 2 cut(s) 376, 931
PspPI GGNCC 2 cut(s) 929, 1456
PstI CTGCAG 2 cut(s) 699, 1360
PstNI CAGNNNCTG 1 cut(s) 1229
PsuI RGATCY 1 cut(s) 487
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 664
SatI GCNGC 4 cut(s) 860, 1038, 1356, 1380
Sau3AI GATC 2 cut(s) 33, 487
Sau96I GGNCC 2 cut(s) 929, 1456
SchI GAGTC 2 cut(s) 1039, 1092
ScrFI CCNGG 3 cut(s) 198, 663, 1391
SduI GDGCHC 1 cut(s) 661
SfaNI GCATC 6 cut(s) 58, 113, 645, 656, 678, 1160
SfcI CTRYAG 3 cut(s) 695, 999, 1356
SinI GGWCC 2 cut(s) 929, 1456
SmiMI CAYNNNNRTG 1 cut(s) 664
SseBI AGGCCT 1 cut(s) 605
SspMI CTAG 3 cut(s) 278, 572, 1304
StuI AGGCCT 1 cut(s) 605
StyD4I CCNGG 3 cut(s) 196, 661, 1389
StyI CCWWGG 1 cut(s) 448
TaaI ACNGT 3 cut(s) 1000, 1225, 1414
TaqI TCGA 3 cut(s) 121, 804, 1145
TfiI GAWTC 4 cut(s) 158, 677, 920, 1235
TscAI CASTG 2 cut(s) 1315, 1330
TseFI GTSAC 2 cut(s) 588, 1403
TseI GCWGC 4 cut(s) 859, 1037, 1355, 1379
Tsp45I GTSAC 2 cut(s) 588, 1403
TspDTI ATGAA 5 cut(s) 243, 320, 584, 1233, 1253
TspRI CASTG 2 cut(s) 1315, 1330
Van91I CCANNNNNTGG 2 cut(s) 559, 1325
VneI GTGCAC 1 cut(s) 657
VpaK11BI GGWCC 2 cut(s) 929, 1456
XagI CCTNNNNNAGG 1 cut(s) 651
XapI RAATTY 2 cut(s) 24, 867
XbaI TCTAGA 1 cut(s) 277
XcmI CCANNNNNNNNNTGG 2 cut(s) 560, 846
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 3 cut(s) 278, 572, 1304
Zsp2I ATGCAT 2 cut(s) 305, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.