Rh3DG002900

Phospholipase A(1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0005852
Physical Location & Seq
Reverse (-)
254208 .. 261213
7006 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG002900.1

Sequence Viewer

Length: 1494 bp
ATGCCGAAGCAAATTCACGAGATCAAGGATTTCCTCCTTACTGCAAGAAGGAAGGATGCGCGTTCTGTCAAAATCAAGAGAACGAAGGACGCTGTGAAGTTCAAGGTTCGGTGCTCAAAGTACCTCTACACCCTTTGCGTGTTTGATTCCGAGAAGGCAGACAAGTTGAAGCAATCTCTTCCTCCAGGTTATACAGAAACTTTAGACAAAGACAATGAAATTGTTGTCCCAGATGATGATTATGGCCTCTATGCAATTGATATTCTAGACCCGTCCCCGTTTGTGAAATGCATACATTTGAAAGAGGTATACTATTTTCATGATATGATCAATATGCTTGTGGGTTGTGGCTATAAGAAGGGAACTACATTGTTTGGATATGGCTATGATTTCCGACAAAGCAATAGAATTGAAAAGTTAATGGAAGATCTTAAAGTGAAATTGGAAACTGCTTACAAGGCTTCTGGTGGTAGAAAAGTAAATATAATTTCGCATTCAATGGGTGGACTGCTAGTCATGTGTTTTATGTCACTTCATCATGAGGCCTTTTCAAAATATGTGAGTAAATGGATTTGCATCGGTTGCCCTTTTCAAGGTGCACCAGGATGCATCAATGATTCTCTTTTAACTGGACTGCAGTTTGTTGAAGGCTTGGAAAGCTTTTTTTTTGTATCAAGGTGGACTATGCACCAGCTGTTGGTTGAGTGTCCATCAATCTACGAGATGTTGGCCAATCCAGATTTTAATTGGAAAAAGCAGCCGGAAATTCAGGTTTGGAGGAAGCATTCTAAGGATGGGGAAACTTCTATTGAACTGGAATCTTATGGTCCCGTTGAAAGTATTACTTTGTTTAGAGAAGCATTGAGACATAATGAGCTAACTTATGATGGTAAAACTATAGCTTTGCCATTTAACTTTGCTATTTCCAGATGGGCTGCCGAGACTCGCCAAGTTCTCAACAATGCCAAACTACCAGATGGAGTCTGCTTCTATAACATTTATGGAATATCCTTTGACACACCTTTTGATGTTTGTTATGGCTCGAAGGCATCTCCAATTGAAGACTTGTCTGAAATATGCCATTCAATGCCTCAGTATTCTTATGTGGATGGAGATGAAACAGTTTCTGCAGAATCAGCAAAGGTGTTGTATCTGACTCTTCATATTATGTTGCAGACTTGTATTCTTAGTTTCTACTTTCTAGCCAGTGTTGAGCCATACTCTGGCTATGACGGCTTTATTTGTAAGTTACTGCTTTGGAGCAAGTTTGATAATGGTTCCGGCCTGCATTGGAGTGAATTAAACTTGTTTCTTCGTATTGCGCAGGCAGATGGATTTGCTGCAGTTGAAAGAGTAGCAATAGCTGCAAGACACCGGGACCTATTAAGTGACAAAACGGTTTTTCAACATATCAAGAAGTGGTTGGGAGTTGAGCAGAAGGTCAACATACATTCCAAGACATCCAGAGTTTCAGATAAGCCTGTGAGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110
Pfam Domains
Protein Families
LCAT

Protein Analysis

497

Amino Acids

56.85

Weight (kDa)

6.94

Isoelectric Point (pI)

46.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L38e PF01781 2 - 65 6.1e-32 Ribosomal L38e protein family
LCAT PF02450 80 - 382 1.2e-23 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1323
AccB7I CCANNNNNTGG 2 cut(s) 697, 1223
AccI GTMKAC 1 cut(s) 309
AccII CGCG 1 cut(s) 61
AcoI YGGCCR 1 cut(s) 729
AcsI RAATTY 2 cut(s) 12, 765
AfaI GTAC 1 cut(s) 122
AfiI CCNNNNNNNGG 3 cut(s) 593, 697, 1223
AhdI GACNNNNNGTC 1 cut(s) 512
AjnI CCWGG 2 cut(s) 184, 601
AluBI AGCT 5 cut(s) 660, 694, 877, 902, 1364
AluI AGCT 5 cut(s) 660, 694, 877, 902, 1364
Alw21I GWGCWC 2 cut(s) 116, 601
Alw26I GTCTC 2 cut(s) 859, 935
Alw44I GTGCAC 1 cut(s) 597
AlwNI CAGNNNCTG 1 cut(s) 1127
AoxI GGCC 4 cut(s) 244, 543, 729, 1282
ApaLI GTGCAC 1 cut(s) 597
ApeKI GCWGC 4 cut(s) 757, 935, 1340, 1364
ApoI RAATTY 2 cut(s) 12, 765
ArsI GACNNNNNNTTYG 2 cut(s) 1007, 1039
AspLEI GCGC 2 cut(s) 61, 1324
AspS9I GGNCC 2 cut(s) 827, 1378
AsuC2I CCSGG 1 cut(s) 1376
AvaII GGWCC 2 cut(s) 827, 1378
BaeGI GKGCMC 1 cut(s) 601
BalI TGGCCA 1 cut(s) 731
BauI CACGAG 1 cut(s) 17
BbsI GAAGAC 1 cut(s) 1068
Bbv12I GWGCWC 2 cut(s) 116, 601
BbvI GCAGC 4 cut(s) 769, 922, 1327, 1351
BccI CCATC 7 cut(s) 718, 788, 881, 924, 971, 1103, 1325
BceAI ACGGC 1 cut(s) 1249
BciT130I CCWGG 2 cut(s) 186, 603
BclI TGATCA 1 cut(s) 327
BcnI CCSGG 1 cut(s) 1376
BcoDI GTCTC 2 cut(s) 859, 935
BfaI CTAG 3 cut(s) 266, 512, 1202
BfmI CTRYAG 4 cut(s) 635, 897, 1128, 1341
BglII AGATCT 1 cut(s) 427
BisI GCNGC 4 cut(s) 758, 936, 1341, 1365
BlsI GCNGC 4 cut(s) 759, 937, 1342, 1366
Bme1390I CCNGG 3 cut(s) 186, 603, 1376
Bme18I GGWCC 2 cut(s) 827, 1378
BmeRI GACNNNNNGTC 1 cut(s) 512
BmgT120I GGNCC 2 cut(s) 827, 1378
BmiI GGNNCC 3 cut(s) 829, 1279, 1379
BmrFI CCNGG 3 cut(s) 186, 603, 1376
BmsI GCATC 5 cut(s) 46, 585, 596, 618, 1058
BpiI GAAGAC 1 cut(s) 1068
BplI GAGNNNNNCTC 2 cut(s) 1205, 1237
BpmI CTGGAG 1 cut(s) 168
BpuMI CCSGG 1 cut(s) 1376
BsaBI GATNNNNATC 1 cut(s) 575
Bsc4I CCNNNNNNNGG 3 cut(s) 593, 697, 1223
Bse1I ACTGG 3 cut(s) 634, 819, 1206
Bse8I GATNNNNATC 1 cut(s) 575
BseBI CCWGG 2 cut(s) 186, 603
BseGI GGATG 5 cut(s) 61, 611, 799, 1114, 1460
BseJI GATNNNNATC 1 cut(s) 575
BseLI CCNNNNNNNGG 3 cut(s) 593, 697, 1223
BseMII CTCAG 1 cut(s) 1106
BseNI ACTGG 3 cut(s) 634, 819, 1206
BseSI GKGCMC 1 cut(s) 601
BseXI GCAGC 4 cut(s) 769, 922, 1327, 1351
Bsh1236I CGCG 1 cut(s) 61
BshFI GGCC 4 cut(s) 246, 545, 731, 1284
BsiHKAI GWGCWC 2 cut(s) 116, 601
BsiSI CCGG 3 cut(s) 761, 1281, 1375
BslFI GGGAC 4 cut(s) 212, 259, 813, 1391
BslI CCNNNNNNNGG 3 cut(s) 593, 697, 1223
BsmAI GTCTC 2 cut(s) 859, 935
BsmFI GGGAC 4 cut(s) 212, 259, 813, 1391
BsmI GAATGC 2 cut(s) 493, 784
BsnI GGCC 4 cut(s) 246, 545, 731, 1284
Bsp1286I GDGCHC 2 cut(s) 116, 601
Bsp143I GATC 3 cut(s) 21, 327, 427
BspANI GGCC 4 cut(s) 246, 545, 731, 1284
BspCNI CTCAG 1 cut(s) 1105
BspFNI CGCG 1 cut(s) 61
BspHI TCATGA 2 cut(s) 319, 538
BspLI GGNNCC 3 cut(s) 829, 1279, 1379
BspMAI CTGCAG 3 cut(s) 639, 1132, 1345
BsrI ACTGG 3 cut(s) 634, 819, 1206
BssMI GATC 3 cut(s) 21, 327, 427
BssNAI GTATAC 1 cut(s) 310
BssSI CACGAG 1 cut(s) 17
Bst1107I GTATAC 1 cut(s) 310
Bst2BI CACGAG 1 cut(s) 17
Bst2UI CCWGG 2 cut(s) 186, 603
Bst4CI ACNGT 2 cut(s) 1123, 1399
Bst6I CTCTTC 2 cut(s) 183, 1164
BstAPI GCANNNNNTGC 2 cut(s) 582, 1364
BstC8I GCNNGC 2 cut(s) 1286, 1326
BstDEI CTNAG 3 cut(s) 789, 1092, 1187
BstENI CCTNNNNNAGG 1 cut(s) 591
BstF5I GGATG 5 cut(s) 61, 611, 799, 1114, 1460
BstFNI CGCG 1 cut(s) 61
BstHHI GCGC 2 cut(s) 61, 1324
BstKTI GATC 3 cut(s) 24, 330, 430
BstMAI GTCTC 2 cut(s) 859, 935
BstMBI GATC 3 cut(s) 21, 327, 427
BstMWI GCNNNNNNNGC 6 cut(s) 458, 582, 657, 1136, 1233, 1364
BstNI CCWGG 2 cut(s) 186, 603
BstSCI CCNGG 3 cut(s) 184, 601, 1374
BstSFI CTRYAG 4 cut(s) 635, 897, 1128, 1341
BstSLI GKGCMC 1 cut(s) 601
BstUI CGCG 1 cut(s) 61
BstV1I GCAGC 4 cut(s) 769, 922, 1327, 1351
BstV2I GAAGAC 1 cut(s) 1068
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
BstZ17I GTATAC 1 cut(s) 310
BsuRI GGCC 4 cut(s) 246, 545, 731, 1284
BtsCI GGATG 5 cut(s) 61, 611, 799, 1114, 1460
BtsIMutI CAGTG 1 cut(s) 1213
Cac8I GCNNGC 2 cut(s) 1286, 1326
CaiI CAGNNNCTG 1 cut(s) 1127
CciI TCATGA 2 cut(s) 319, 538
CfoI GCGC 2 cut(s) 61, 1324
Cfr13I GGNCC 2 cut(s) 827, 1378
CseI GACGC 1 cut(s) 98
Csp6I GTAC 1 cut(s) 121
CviAII CATG 3 cut(s) 320, 517, 539
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 3 cut(s) 789, 1092, 1187
DpnI GATC 3 cut(s) 23, 329, 429
DpnII GATC 3 cut(s) 21, 327, 427
DriI GACNNNNNGTC 1 cut(s) 512
EaeI YGGCCR 1 cut(s) 729
Eam1104I CTCTTC 2 cut(s) 183, 1164
Eam1105I GACNNNNNGTC 1 cut(s) 512
EarI CTCTTC 2 cut(s) 183, 1164
Eco147I AGGCCT 1 cut(s) 545
Eco47I GGWCC 2 cut(s) 827, 1378
EcoNI CCTNNNNNAGG 1 cut(s) 591
EcoO109I RGGNCCY 1 cut(s) 1378
EcoRII CCWGG 2 cut(s) 184, 601
EcoT22I ATGCAT 2 cut(s) 293, 611
FaeI CATG 3 cut(s) 323, 520, 542
FalI AAGNNNNNCTT 2 cut(s) 829, 861
FaqI GGGAC 4 cut(s) 212, 259, 813, 1391
FatI CATG 3 cut(s) 319, 516, 538
FbaI TGATCA 1 cut(s) 327
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 4 cut(s) 758, 936, 1341, 1365
FokI GGATG 5 cut(s) 68, 618, 806, 1121, 1447
Fsp4HI GCNGC 4 cut(s) 758, 936, 1341, 1365
FspBI CTAG 3 cut(s) 266, 512, 1202
FspI TGCGCA 1 cut(s) 1323
GlaI GCGC 2 cut(s) 60, 1323
GluI GCNGC 4 cut(s) 758, 936, 1341, 1365
GsuI CTGGAG 1 cut(s) 168
HaeIII GGCC 4 cut(s) 246, 545, 731, 1284
HapII CCGG 3 cut(s) 761, 1281, 1375
HgaI GACGC 1 cut(s) 98
HhaI GCGC 2 cut(s) 61, 1324
Hin1II CATG 3 cut(s) 323, 520, 542
Hin6I GCGC 2 cut(s) 59, 1322
HinP1I GCGC 2 cut(s) 59, 1322
HincII GTYRAC 1 cut(s) 1444
HindII GTYRAC 1 cut(s) 1444
HindIII AAGCTT 1 cut(s) 658
HinfI GANTC 7 cut(s) 146, 617, 818, 943, 981, 1133, 1156
HpaII CCGG 3 cut(s) 761, 1281, 1375
Hpy166II GTNNAC 5 cut(s) 310, 506, 599, 681, 1444
Hpy188I TCNGA 5 cut(s) 151, 395, 1072, 1155, 1474
Hpy188III TCNNGA 9 cut(s) 17, 76, 266, 320, 539, 737, 927, 1414, 1464
Hpy8I GTNNAC 5 cut(s) 310, 506, 599, 681, 1444
HpyAV CCTTC 8 cut(s) 42, 46, 79, 148, 352, 641, 1039, 1432
HpyCH4III ACNGT 2 cut(s) 1123, 1399
HpyF10VI GCNNNNNNNGC 6 cut(s) 458, 582, 657, 1136, 1233, 1364
HpyF3I CTNAG 3 cut(s) 789, 1092, 1187
Hsp92II CATG 3 cut(s) 323, 520, 542
HspAI GCGC 2 cut(s) 59, 1322
Ksp22I TGATCA 1 cut(s) 327
Kzo9I GATC 3 cut(s) 21, 327, 427
LmnI GCTCC 1 cut(s) 1260
Lsp1109I GCAGC 4 cut(s) 769, 922, 1327, 1351
LweI GCATC 5 cut(s) 46, 585, 596, 618, 1058
MaeI CTAG 3 cut(s) 266, 512, 1202
MaeIII GTNAC 3 cut(s) 528, 1248, 1388
MalI GATC 3 cut(s) 23, 329, 429
MboI GATC 3 cut(s) 21, 327, 427
MboII GAAGA 5 cut(s) 170, 437, 1073, 1151, 1304
MfeI CAATTG 2 cut(s) 255, 1056
MflI RGATCY 1 cut(s) 427
MhlI GDGCHC 2 cut(s) 116, 601
MlsI TGGCCA 1 cut(s) 731
MluNI TGGCCA 1 cut(s) 731
MlyI GAGTC 3 cut(s) 937, 990, 1150
MmeI TCCRAC 1 cut(s) 418
MnlI CCTC 9 cut(s) 44, 134, 192, 257, 298, 535, 771, 1101, 1479
Mox20I TGGCCA 1 cut(s) 731
Mph1103I ATGCAT 2 cut(s) 293, 611
MscI TGGCCA 1 cut(s) 731
MseI TTAA 7 cut(s) 419, 432, 626, 744, 912, 1301, 1385
MslI CAYNNNNRTG 2 cut(s) 604, 1293
Msp20I TGGCCA 1 cut(s) 731
MspA1I CMGCKG 1 cut(s) 694
MspI CCGG 3 cut(s) 761, 1281, 1375
MspR9I CCNGG 3 cut(s) 186, 603, 1376
MunI CAATTG 2 cut(s) 255, 1056
Mva1269I GAATGC 2 cut(s) 493, 784
MvaI CCWGG 2 cut(s) 186, 603
MvnI CGCG 1 cut(s) 61
MwoI GCNNNNNNNGC 6 cut(s) 458, 582, 657, 1136, 1233, 1364
NciI CCSGG 1 cut(s) 1376
NdeII GATC 3 cut(s) 21, 327, 427
NlaIII CATG 3 cut(s) 323, 520, 542
NlaIV GGNNCC 3 cut(s) 829, 1279, 1379
NmeAIII GCCGAG 1 cut(s) 964
NmuCI GTSAC 2 cut(s) 528, 1388
NsbI TGCGCA 1 cut(s) 1323
NsiI ATGCAT 2 cut(s) 293, 611
PagI TCATGA 2 cut(s) 319, 538
PceI AGGCCT 1 cut(s) 545
PctI GAATGC 2 cut(s) 493, 784
PfeI GAWTC 4 cut(s) 146, 617, 818, 1133
PflMI CCANNNNNTGG 2 cut(s) 697, 1223
PkrI GCNGC 4 cut(s) 759, 937, 1342, 1366
PleI GAGTC 3 cut(s) 937, 989, 1150
PpsI GAGTC 3 cut(s) 937, 989, 1150
PpuMI RGGWCCY 1 cut(s) 1378
Psp5II RGGWCCY 1 cut(s) 1378
Psp6I CCWGG 2 cut(s) 184, 601
PspGI CCWGG 2 cut(s) 184, 601
PspN4I GGNNCC 3 cut(s) 829, 1279, 1379
PspPI GGNCC 2 cut(s) 827, 1378
PspPPI RGGWCCY 1 cut(s) 1378
PstI CTGCAG 3 cut(s) 639, 1132, 1345
PstNI CAGNNNCTG 1 cut(s) 1127
PsuI RGATCY 1 cut(s) 427
PvuII CAGCTG 1 cut(s) 694
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
RseI CAYNNNNRTG 2 cut(s) 604, 1293
SaqAI TTAA 7 cut(s) 419, 432, 626, 744, 912, 1301, 1385
SatI GCNGC 4 cut(s) 758, 936, 1341, 1365
Sau3AI GATC 3 cut(s) 21, 327, 427
Sau96I GGNCC 2 cut(s) 827, 1378
SchI GAGTC 3 cut(s) 937, 990, 1150
ScrFI CCNGG 3 cut(s) 186, 603, 1376
SduI GDGCHC 2 cut(s) 116, 601
SfaNI GCATC 5 cut(s) 46, 585, 596, 618, 1058
SfcI CTRYAG 4 cut(s) 635, 897, 1128, 1341
SinI GGWCC 2 cut(s) 827, 1378
SmiMI CAYNNNNRTG 2 cut(s) 604, 1293
SseBI AGGCCT 1 cut(s) 545
SspMI CTAG 3 cut(s) 266, 512, 1202
StuI AGGCCT 1 cut(s) 545
StyD4I CCNGG 3 cut(s) 184, 601, 1374
TaaI ACNGT 2 cut(s) 1123, 1399
TaqI TCGA 1 cut(s) 1043
TfiI GAWTC 4 cut(s) 146, 617, 818, 1133
Tru1I TTAA 7 cut(s) 419, 432, 626, 744, 912, 1301, 1385
Tru9I TTAA 7 cut(s) 419, 432, 626, 744, 912, 1301, 1385
TscAI CASTG 1 cut(s) 1213
TseFI GTSAC 2 cut(s) 528, 1388
TseI GCWGC 4 cut(s) 757, 935, 1340, 1364
Tsp45I GTSAC 2 cut(s) 528, 1388
TspDTI ATGAA 5 cut(s) 231, 308, 524, 1131, 1151
TspRI CASTG 1 cut(s) 1213
Van91I CCANNNNNTGG 2 cut(s) 697, 1223
VneI GTGCAC 1 cut(s) 597
VpaK11BI GGWCC 2 cut(s) 827, 1378
XagI CCTNNNNNAGG 1 cut(s) 591
XapI RAATTY 2 cut(s) 12, 765
XbaI TCTAGA 1 cut(s) 265
XcmI CCANNNNNNNNNTGG 1 cut(s) 744
XmiI GTMKAC 1 cut(s) 309
XspI CTAG 3 cut(s) 266, 512, 1202
Zsp2I ATGCAT 2 cut(s) 293, 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.