Rh3BG002500

Phospholipase A(1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0005852
Physical Location & Seq
Reverse (-)
254449 .. 258140
3692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG002500.1

Sequence Viewer

Length: 1062 bp
ATGGTTATATCTCTCTCTCCATGTTTCCAGTTTGTGAAATGCATACATTTGAAAGAGGTATACTATTTTCATGATATGATCAATATGCTTGTGGGTTGTGGCTATAAGAAGGGAACTACATTGTTTGGATATGGCTATGATTTCCGACAAAGCAATAGAATTGAAAAGTTAATGGAAGATCTTAAAGTGAAATTGGAAACTGCTTACAAGGCTTCTGGTGGTAGAAAAGTAAATATAATTTCGCATTCAATGGGTGGACTGCTAGTCATGTGTTTTATGTCACTTCATCATGAGGCCTTTTCAAAATATGTGAGTAAATGGATTTGCATCGGTTGCCCTTTTCAAGGTGCACCAGGATGCATCAATGATTCTCTTTTAACTGGACTGCAGTTTGTTGAAGGCTTGGAAAGCTTTTTTTTTGTATCAAGGTGGACTATGCACCAGCTGTTGGTTGAGTGTCCATCAATCTACGAGATGTTGGCCAATCCAGATTTTAATTGGAAAAAGCAGCCGGAAATTCAGGTTTGGAGGAAGCATTCTAAGGATGGGGAAACTTCTATTGAACTGGAATCTTATGGTCCCGTTGAAAGTATTACTTTGTTTAGAGAAGCATTGAGACATAATGAGCTAACTTATGATGGTAAAACTATAGCTTTGCCATTTAACTTTGCTATTTCCAGATGGGCTGCCGAGACTCGCCAAGTTCTCAACAATGCCAAACTACCAGATGGAGTCTGCTTCTATAACATTTATGGAATATCCTTTGACACACCTTTTGATGTTTGTTATGGCTCGAAGGCATCTCCAATTGAAGACTTGTCTGAAATATGCCATTCAATGCCTCAGTATTCTTATGTGGATGGAGATGAAACAGTTTCTGCAGAATCAGCAAAGGCAGATGGATTTGCTGCAGTTGAAAGAGTAGCAATAGCTGCAAGACACCGGGACCTATTAAGTGACAAAACGGTTTTTCAACATATCAAGAAGTGGTTGGGAGTTGAGCAGAAGGTCAACATACATTCCAAGACATCCAGAGTTTCAGATAAGCCTGTGAGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110
Pfam Domains
Protein Families
LCAT

Protein Analysis

353

Amino Acids

40.15

Weight (kDa)

6.64

Isoelectric Point (pI)

45.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 19 - 297 2.2e-21 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 448
AccI GTMKAC 1 cut(s) 60
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 1 cut(s) 516
AfiI CCNNNNNNNGG 2 cut(s) 344, 448
AhdI GACNNNNNGTC 1 cut(s) 263
AjnI CCWGG 1 cut(s) 352
AluBI AGCT 5 cut(s) 411, 445, 628, 653, 932
AluI AGCT 5 cut(s) 411, 445, 628, 653, 932
Alw21I GWGCWC 1 cut(s) 352
Alw26I GTCTC 2 cut(s) 610, 686
Alw44I GTGCAC 1 cut(s) 348
AlwNI CAGNNNCTG 1 cut(s) 878
AoxI GGCC 2 cut(s) 294, 480
ApaLI GTGCAC 1 cut(s) 348
ApeKI GCWGC 4 cut(s) 508, 686, 908, 932
ApoI RAATTY 1 cut(s) 516
ArsI GACNNNNNNTTYG 2 cut(s) 758, 790
AspS9I GGNCC 2 cut(s) 578, 946
AsuC2I CCSGG 1 cut(s) 944
AvaII GGWCC 2 cut(s) 578, 946
BaeGI GKGCMC 1 cut(s) 352
BalI TGGCCA 1 cut(s) 482
BbsI GAAGAC 1 cut(s) 819
Bbv12I GWGCWC 1 cut(s) 352
BbvI GCAGC 4 cut(s) 520, 673, 895, 919
BccI CCATC 7 cut(s) 469, 539, 632, 675, 722, 854, 893
BciT130I CCWGG 1 cut(s) 354
BclI TGATCA 1 cut(s) 78
BcnI CCSGG 1 cut(s) 944
BcoDI GTCTC 2 cut(s) 610, 686
BfaI CTAG 1 cut(s) 263
BfmI CTRYAG 4 cut(s) 386, 648, 879, 909
BglII AGATCT 1 cut(s) 178
BisI GCNGC 4 cut(s) 509, 687, 909, 933
BlsI GCNGC 4 cut(s) 510, 688, 910, 934
Bme1390I CCNGG 2 cut(s) 354, 944
Bme18I GGWCC 2 cut(s) 578, 946
BmeRI GACNNNNNGTC 1 cut(s) 263
BmgT120I GGNCC 2 cut(s) 578, 946
BmiI GGNNCC 2 cut(s) 580, 947
BmrFI CCNGG 2 cut(s) 354, 944
BmsI GCATC 4 cut(s) 336, 347, 369, 809
BpiI GAAGAC 1 cut(s) 819
BpuMI CCSGG 1 cut(s) 944
BsaBI GATNNNNATC 1 cut(s) 326
Bsc4I CCNNNNNNNGG 2 cut(s) 344, 448
Bse1I ACTGG 3 cut(s) 28, 385, 570
Bse8I GATNNNNATC 1 cut(s) 326
BseBI CCWGG 1 cut(s) 354
BseGI GGATG 4 cut(s) 362, 550, 865, 1028
BseJI GATNNNNATC 1 cut(s) 326
BseLI CCNNNNNNNGG 2 cut(s) 344, 448
BseMII CTCAG 1 cut(s) 857
BseNI ACTGG 3 cut(s) 28, 385, 570
BseSI GKGCMC 1 cut(s) 352
BseXI GCAGC 4 cut(s) 520, 673, 895, 919
BshFI GGCC 2 cut(s) 296, 482
BsiHKAI GWGCWC 1 cut(s) 352
BsiSI CCGG 2 cut(s) 512, 943
BslFI GGGAC 2 cut(s) 564, 959
BslI CCNNNNNNNGG 2 cut(s) 344, 448
BsmAI GTCTC 2 cut(s) 610, 686
BsmFI GGGAC 2 cut(s) 564, 959
BsmI GAATGC 2 cut(s) 244, 535
BsnI GGCC 2 cut(s) 296, 482
Bsp1286I GDGCHC 1 cut(s) 352
Bsp143I GATC 2 cut(s) 78, 178
BspANI GGCC 2 cut(s) 296, 482
BspCNI CTCAG 1 cut(s) 856
BspHI TCATGA 2 cut(s) 70, 289
BspLI GGNNCC 2 cut(s) 580, 947
BspMAI CTGCAG 3 cut(s) 390, 883, 913
BsrI ACTGG 3 cut(s) 28, 385, 570
BssMI GATC 2 cut(s) 78, 178
BssNAI GTATAC 1 cut(s) 61
Bst1107I GTATAC 1 cut(s) 61
Bst2UI CCWGG 1 cut(s) 354
Bst4CI ACNGT 2 cut(s) 874, 967
BstAPI GCANNNNNTGC 2 cut(s) 333, 932
BstDEI CTNAG 2 cut(s) 540, 843
BstENI CCTNNNNNAGG 1 cut(s) 342
BstF5I GGATG 4 cut(s) 362, 550, 865, 1028
BstKTI GATC 2 cut(s) 81, 181
BstMAI GTCTC 2 cut(s) 610, 686
BstMBI GATC 2 cut(s) 78, 178
BstMWI GCNNNNNNNGC 5 cut(s) 209, 333, 408, 887, 932
BstNI CCWGG 1 cut(s) 354
BstSCI CCNGG 2 cut(s) 352, 942
BstSFI CTRYAG 4 cut(s) 386, 648, 879, 909
BstSLI GKGCMC 1 cut(s) 352
BstV1I GCAGC 4 cut(s) 520, 673, 895, 919
BstV2I GAAGAC 1 cut(s) 819
BstX2I RGATCY 1 cut(s) 178
BstYI RGATCY 1 cut(s) 178
BstZ17I GTATAC 1 cut(s) 61
BsuRI GGCC 2 cut(s) 296, 482
BtsCI GGATG 4 cut(s) 362, 550, 865, 1028
CaiI CAGNNNCTG 1 cut(s) 878
CciI TCATGA 2 cut(s) 70, 289
Cfr13I GGNCC 2 cut(s) 578, 946
CviAII CATG 4 cut(s) 21, 71, 268, 290
DdeI CTNAG 2 cut(s) 540, 843
DpnI GATC 2 cut(s) 80, 180
DpnII GATC 2 cut(s) 78, 178
DriI GACNNNNNGTC 1 cut(s) 263
EaeI YGGCCR 1 cut(s) 480
Eam1105I GACNNNNNGTC 1 cut(s) 263
Eco147I AGGCCT 1 cut(s) 296
Eco47I GGWCC 2 cut(s) 578, 946
EcoNI CCTNNNNNAGG 1 cut(s) 342
EcoO109I RGGNCCY 1 cut(s) 946
EcoRII CCWGG 1 cut(s) 352
EcoT22I ATGCAT 2 cut(s) 44, 362
FaeI CATG 4 cut(s) 24, 74, 271, 293
FalI AAGNNNNNCTT 2 cut(s) 580, 612
FaqI GGGAC 2 cut(s) 564, 959
FatI CATG 4 cut(s) 20, 70, 267, 289
FbaI TGATCA 1 cut(s) 78
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 4 cut(s) 509, 687, 909, 933
FokI GGATG 4 cut(s) 369, 557, 872, 1015
Fsp4HI GCNGC 4 cut(s) 509, 687, 909, 933
FspBI CTAG 1 cut(s) 263
GluI GCNGC 4 cut(s) 509, 687, 909, 933
HaeIII GGCC 2 cut(s) 296, 482
HapII CCGG 2 cut(s) 512, 943
Hin1II CATG 4 cut(s) 24, 74, 271, 293
HincII GTYRAC 1 cut(s) 1012
HindII GTYRAC 1 cut(s) 1012
HindIII AAGCTT 1 cut(s) 409
HinfI GANTC 5 cut(s) 368, 569, 694, 732, 884
HpaII CCGG 2 cut(s) 512, 943
Hpy166II GTNNAC 5 cut(s) 61, 257, 350, 432, 1012
Hpy188I TCNGA 3 cut(s) 146, 823, 1042
Hpy188III TCNNGA 6 cut(s) 71, 290, 488, 678, 982, 1032
Hpy8I GTNNAC 5 cut(s) 61, 257, 350, 432, 1012
HpyAV CCTTC 4 cut(s) 103, 392, 790, 1000
HpyCH4III ACNGT 2 cut(s) 874, 967
HpyCH4V TGCA 9 cut(s) 42, 327, 350, 360, 388, 439, 881, 911, 935
HpyF10VI GCNNNNNNNGC 5 cut(s) 209, 333, 408, 887, 932
HpyF3I CTNAG 2 cut(s) 540, 843
Hsp92II CATG 4 cut(s) 24, 74, 271, 293
Ksp22I TGATCA 1 cut(s) 78
Kzo9I GATC 2 cut(s) 78, 178
Lsp1109I GCAGC 4 cut(s) 520, 673, 895, 919
LweI GCATC 4 cut(s) 336, 347, 369, 809
MaeI CTAG 1 cut(s) 263
MaeIII GTNAC 2 cut(s) 279, 956
MalI GATC 2 cut(s) 80, 180
MboI GATC 2 cut(s) 78, 178
MboII GAAGA 2 cut(s) 188, 824
MfeI CAATTG 1 cut(s) 807
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 352
MlsI TGGCCA 1 cut(s) 482
MluCI AATT 6 cut(s) 159, 191, 237, 496, 516, 807
MluNI TGGCCA 1 cut(s) 482
MlyI GAGTC 2 cut(s) 688, 741
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 5 cut(s) 49, 286, 522, 852, 1047
Mox20I TGGCCA 1 cut(s) 482
Mph1103I ATGCAT 2 cut(s) 44, 362
MscI TGGCCA 1 cut(s) 482
MseI TTAA 6 cut(s) 170, 183, 377, 495, 663, 953
MslI CAYNNNNRTG 1 cut(s) 355
Msp20I TGGCCA 1 cut(s) 482
MspA1I CMGCKG 1 cut(s) 445
MspI CCGG 2 cut(s) 512, 943
MspR9I CCNGG 2 cut(s) 354, 944
MunI CAATTG 1 cut(s) 807
Mva1269I GAATGC 2 cut(s) 244, 535
MvaI CCWGG 1 cut(s) 354
MwoI GCNNNNNNNGC 5 cut(s) 209, 333, 408, 887, 932
NciI CCSGG 1 cut(s) 944
NdeII GATC 2 cut(s) 78, 178
NlaIII CATG 4 cut(s) 24, 74, 271, 293
NlaIV GGNNCC 2 cut(s) 580, 947
NmeAIII GCCGAG 1 cut(s) 715
NmuCI GTSAC 2 cut(s) 279, 956
NsiI ATGCAT 2 cut(s) 44, 362
PagI TCATGA 2 cut(s) 70, 289
PceI AGGCCT 1 cut(s) 296
PctI GAATGC 2 cut(s) 244, 535
PfeI GAWTC 3 cut(s) 368, 569, 884
PflMI CCANNNNNTGG 1 cut(s) 448
PkrI GCNGC 4 cut(s) 510, 688, 910, 934
PleI GAGTC 2 cut(s) 688, 740
PpsI GAGTC 2 cut(s) 688, 740
PpuMI RGGWCCY 1 cut(s) 946
Psp5II RGGWCCY 1 cut(s) 946
Psp6I CCWGG 1 cut(s) 352
PspGI CCWGG 1 cut(s) 352
PspN4I GGNNCC 2 cut(s) 580, 947
PspPI GGNCC 2 cut(s) 578, 946
PspPPI RGGWCCY 1 cut(s) 946
PstI CTGCAG 3 cut(s) 390, 883, 913
PstNI CAGNNNCTG 1 cut(s) 878
PsuI RGATCY 1 cut(s) 178
PvuII CAGCTG 1 cut(s) 445
RseI CAYNNNNRTG 1 cut(s) 355
SaqAI TTAA 6 cut(s) 170, 183, 377, 495, 663, 953
SatI GCNGC 4 cut(s) 509, 687, 909, 933
Sau3AI GATC 2 cut(s) 78, 178
Sau96I GGNCC 2 cut(s) 578, 946
SchI GAGTC 2 cut(s) 688, 741
ScrFI CCNGG 2 cut(s) 354, 944
SduI GDGCHC 1 cut(s) 352
SfaNI GCATC 4 cut(s) 336, 347, 369, 809
SfcI CTRYAG 4 cut(s) 386, 648, 879, 909
SinI GGWCC 2 cut(s) 578, 946
SmiMI CAYNNNNRTG 1 cut(s) 355
Sse9I AATT 6 cut(s) 159, 191, 237, 496, 516, 807
SseBI AGGCCT 1 cut(s) 296
SspMI CTAG 1 cut(s) 263
StuI AGGCCT 1 cut(s) 296
StyD4I CCNGG 2 cut(s) 352, 942
TaaI ACNGT 2 cut(s) 874, 967
TaqI TCGA 1 cut(s) 794
TasI AATT 6 cut(s) 159, 191, 237, 496, 516, 807
TfiI GAWTC 3 cut(s) 368, 569, 884
Tru1I TTAA 6 cut(s) 170, 183, 377, 495, 663, 953
Tru9I TTAA 6 cut(s) 170, 183, 377, 495, 663, 953
TseFI GTSAC 2 cut(s) 279, 956
TseI GCWGC 4 cut(s) 508, 686, 908, 932
Tsp45I GTSAC 2 cut(s) 279, 956
TspDTI ATGAA 3 cut(s) 59, 275, 882
Van91I CCANNNNNTGG 1 cut(s) 448
VneI GTGCAC 1 cut(s) 348
VpaK11BI GGWCC 2 cut(s) 578, 946
XagI CCTNNNNNAGG 1 cut(s) 342
XapI RAATTY 1 cut(s) 516
XcmI CCANNNNNNNNNTGG 1 cut(s) 495
XmiI GTMKAC 1 cut(s) 60
XspI CTAG 1 cut(s) 263
Zsp2I ATGCAT 2 cut(s) 44, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.