MD04G1247600.v1.1

Phospholipase A(1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005852
Physical Location & Seq
Forward (+)
32217920 .. 32222392
4473 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1247600.v1.1.491

Sequence Viewer

Length: 1359 bp
ATGACAGGCCGCTGCTTCTGCCCCTGTTTCGACTTCGAACACGAGAACCGCACCTCCTCCGACACGGAGGTGGAGCCGGTGCTGCTGGTTTCTGGCATAGGAGGCTCCATTCTGCACTCCAAGAGGAGGAAGTTGGGGTTCGACACCCGGGTCTGGGTCCGGATCTTATTGGCTGACTTGGAGTTCAAGAAGAAGCTATGGTCTATCTACAATCCCCAAACAGGTTATACTGAAACGCTAGACAAAGACACTGAAATTGTGGTCCCAGATGACGATTACGGCCTGTATGCAATTGATATTCTAGATCCTTCCTGGTTTGTCAAATTGATACGTTTGAAAGAGGTATACCAGTTCCATGATATGATTGATATGCTTGTCGGATGTGGATATAAGAAGGGAGCCACTTTGTTTGGATACGGATATGATTTTCGACAAAGCAATAGAATTGACAAGTTAATGGAAGGTCTTAAGGTGAAACTGGAAACTGCTTACAAGGCTTCTGGGGGTAGAAAAGTGAATATAATTTCGCATTCAATGGGTGGACTGCTAGTCACATGTTTTATGTCACTCCATAATGATGTATTTTCGAAGTACGTGAGTAAATGGATCTGCATAGCTTGCCCTTTTCAAGGTGCACCAGGATGCATCAATGATTCTCTCTTAACTGGATTACAGTTTGTTGAAGGCTTGGAAAGTTACTTTTTTGTATCAAGGTGGACTATGCACCAGCTGTTGGTTGAGTGTCCATCAATCTATGAGATGTTGGCAAATCCAAAATTTAAATGGAAAAGGCGCCCAGAAATTCAGGTTTGGAGGAACAATTCCACAGATGGGGAAACTTTGGTTGATTTGGAGTCTTATGGCCCAATTGAAAGCATTCCTTTGTTTGAAGAAGCATTGAAACATAATGAGCTAAATTATGATGGCAAAACGGTAGCTTTGCCATTTAACCATTCTATTCTCAGATGGGCTGCTGAGACTCACCAAGTTTTGAACAGTGCTAAACTACCAGATAGCGTCAGCTTCTATAACATTTATGGCACATCGTTCGACACCCCTTTTGATGTTTGTTACGGTTCGGAGACATCTCCAATACAGGACCTGTCTGAAATATGCCATTCAATTCCTCAGTATTCTTATGTGGATGGAGATGAAACGGTTCCGGCTGAGTCAGCAAAGGCAGATGGATTTGCTGCAGTGGAAAGAGTAGCCATAGCTGCAAGGCACCGGGAATTATTGCGGGACAAAACAGTTTTTCAACATATCCAAAGGTGGTTGGGAGTCGAACAGAAGGTCGGCAGACATTCTAAGATATCCAAAGTTGCAGACGCCTCTTCAAATAAGCATGCAAATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110
Pfam Domains
Protein Families
LCAT

Protein Analysis

453

Amino Acids

51.34

Weight (kDa)

5.99

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 69 - 393 1.4e-25 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
AccB1I GGYRCC 2 cut(s) 792, 1224
AccB7I CCANNNNNTGG 1 cut(s) 733
AccI GTMKAC 1 cut(s) 345
AccIII TCCGGA 1 cut(s) 159
AciI CCGC 3 cut(s) 10, 49, 1240
AclWI GGATC 3 cut(s) 170, 299, 614
AcsI RAATTY 2 cut(s) 776, 801
AcyI GRCGYC 2 cut(s) 793, 1329
AfaI GTAC 1 cut(s) 593
AfiI CCNNNNNNNGG 7 cut(s) 126, 153, 154, 221, 629, 733, 831
AflII CTTAAG 1 cut(s) 467
AflIII ACRYGT 1 cut(s) 554
AhdI GACNNNNNGTC 1 cut(s) 548
AjnI CCWGG 2 cut(s) 311, 637
AleI CACNNNNGTG 1 cut(s) 68
AloI GAACNNNNNNTCC 2 cut(s) 38, 70
AluBI AGCT 7 cut(s) 196, 617, 730, 913, 938, 1023, 1217
AluI AGCT 7 cut(s) 196, 617, 730, 913, 938, 1023, 1217
Alw21I GWGCWC 1 cut(s) 637
Alw26I GTCTC 2 cut(s) 971, 1076
Alw44I GTGCAC 1 cut(s) 633
AlwI GGATC 3 cut(s) 170, 299, 614
AlwNI CAGNNNCTG 1 cut(s) 1102
Ama87I CYCGRG 1 cut(s) 147
Aor13HI TCCGGA 1 cut(s) 159
AoxI GGCC 3 cut(s) 7, 280, 862
ApaLI GTGCAC 1 cut(s) 633
ApeKI GCWGC 5 cut(s) 12, 82, 971, 1193, 1217
ApoI RAATTY 2 cut(s) 776, 801
ArsI GACNNNNNNTTYG 4 cut(s) 1043, 1075, 1278, 1310
Asp700I GAANNNNTTC 2 cut(s) 876, 1158
AspLEI GCGC 1 cut(s) 795
AspS9I GGNCC 4 cut(s) 157, 262, 863, 1099
AsuC2I CCSGG 3 cut(s) 148, 149, 1229
AsuHPI GGTGA 2 cut(s) 484, 974
AsuII TTCGAA 2 cut(s) 36, 587
AvaI CYCGRG 1 cut(s) 147
AvaII GGWCC 3 cut(s) 157, 262, 1099
BaeGI GKGCMC 1 cut(s) 637
BanI GGYRCC 2 cut(s) 792, 1224
BauI CACGAG 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 637
BbvI GCAGC 4 cut(s) 69, 958, 1180, 1204
BccI CCATC 6 cut(s) 754, 824, 917, 960, 1139, 1178
BceAI ACGGC 1 cut(s) 295
BcgI CGANNNNNNTGC 2 cut(s) 1030, 1064
BciT130I CCWGG 2 cut(s) 313, 639
BciVI GTATCC 1 cut(s) 407
BcnI CCSGG 3 cut(s) 148, 149, 1229
BcoDI GTCTC 2 cut(s) 971, 1076
BfaI CTAG 3 cut(s) 239, 302, 548
BfmI CTRYAG 1 cut(s) 1194
BfoI RGCGCY 1 cut(s) 796
BfrI CTTAAG 1 cut(s) 467
BfuI GTATCC 1 cut(s) 407
BisI GCNGC 6 cut(s) 10, 13, 83, 972, 1194, 1218
BlsI GCNGC 6 cut(s) 11, 14, 84, 973, 1195, 1219
Bme1390I CCNGG 5 cut(s) 148, 149, 313, 639, 1229
Bme18I GGWCC 3 cut(s) 157, 262, 1099
BmeRI GACNNNNNGTC 1 cut(s) 548
BmeT110I CYCGRG 1 cut(s) 147
BmgT120I GGNCC 4 cut(s) 157, 262, 863, 1099
BmiI GGNNCC 8 cut(s) 75, 106, 158, 264, 400, 794, 1161, 1226
BmrFI CCNGG 5 cut(s) 148, 149, 313, 639, 1229
BmsI GCATC 2 cut(s) 632, 654
Bpu14I TTCGAA 2 cut(s) 36, 587
BpuMI CCSGG 3 cut(s) 148, 149, 1229
BsaAI YACGTR 1 cut(s) 595
BsaHI GRCGYC 2 cut(s) 793, 1329
BsaJI CCNNGG 1 cut(s) 147
BsaWI WCCGGW 1 cut(s) 159
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 7 cut(s) 126, 153, 154, 221, 629, 733, 831
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 3 cut(s) 349, 483, 670
BseAI TCCGGA 1 cut(s) 159
BseBI CCWGG 2 cut(s) 313, 639
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 3 cut(s) 386, 647, 1150
BseLI CCNNNNNNNGG 7 cut(s) 126, 153, 154, 221, 629, 733, 831
BseMII CTCAG 4 cut(s) 966, 976, 1142, 1158
BseNI ACTGG 3 cut(s) 349, 483, 670
BseRI GAGGAG 2 cut(s) 46, 139
BseSI GKGCMC 1 cut(s) 637
BseXI GCAGC 4 cut(s) 69, 958, 1180, 1204
BsgI GTGCAG 1 cut(s) 98
BshFI GGCC 3 cut(s) 9, 282, 864
BshNI GGYRCC 2 cut(s) 792, 1224
BsiHKAI GWGCWC 1 cut(s) 637
BsiHKCI CYCGRG 1 cut(s) 147
BsiSI CCGG 5 cut(s) 77, 148, 160, 1163, 1228
BslFI GGGAC 2 cut(s) 248, 1256
BslI CCNNNNNNNGG 7 cut(s) 126, 153, 154, 221, 629, 733, 831
BsmAI GTCTC 2 cut(s) 971, 1076
BsmFI GGGAC 2 cut(s) 248, 1256
BsmI GAATGC 2 cut(s) 529, 876
BsnI GGCC 3 cut(s) 9, 282, 864
BsoBI CYCGRG 1 cut(s) 147
Bsp119I TTCGAA 2 cut(s) 36, 587
Bsp1286I GDGCHC 1 cut(s) 637
Bsp13I TCCGGA 1 cut(s) 159
Bsp143I GATC 3 cut(s) 162, 304, 606
BspACI CCGC 3 cut(s) 10, 49, 1240
BspANI GGCC 3 cut(s) 9, 282, 864
BspCNI CTCAG 4 cut(s) 967, 975, 1141, 1159
BspEI TCCGGA 1 cut(s) 159
BspLI GGNNCC 8 cut(s) 75, 106, 158, 264, 400, 794, 1161, 1226
BspMAI CTGCAG 1 cut(s) 1198
BspPI GGATC 3 cut(s) 170, 299, 614
BspT104I TTCGAA 2 cut(s) 36, 587
BspT107I GGYRCC 2 cut(s) 792, 1224
BspTI CTTAAG 1 cut(s) 467
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 3 cut(s) 349, 483, 670
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 3 cut(s) 162, 304, 606
BssNAI GTATAC 1 cut(s) 346
BssNI GRCGYC 2 cut(s) 793, 1329
BssSI CACGAG 1 cut(s) 41
Bst1107I GTATAC 1 cut(s) 346
Bst2BI CACGAG 1 cut(s) 41
Bst2UI CCWGG 2 cut(s) 313, 639
Bst4CI ACNGT 6 cut(s) 675, 934, 998, 1076, 1159, 1252
Bst6I CTCTTC 1 cut(s) 1339
BstACI GRCGYC 2 cut(s) 793, 1329
BstAFI CTTAAG 1 cut(s) 467
BstAPI GCANNNNNTGC 1 cut(s) 618
BstBAI YACGTR 1 cut(s) 595
BstBI TTCGAA 2 cut(s) 36, 587
BstC8I GCNNGC 2 cut(s) 619, 1347
BstDEI CTNAG 5 cut(s) 962, 975, 1128, 1167, 1308
BstENI CCTNNNNNAGG 1 cut(s) 627
BstF5I GGATG 3 cut(s) 386, 647, 1150
BstH2I RGCGCY 1 cut(s) 796
BstHHI GCGC 1 cut(s) 795
BstKTI GATC 3 cut(s) 165, 307, 609
BstMAI GTCTC 2 cut(s) 971, 1076
BstMBI GATC 3 cut(s) 162, 304, 606
BstMWI GCNNNNNNNGC 7 cut(s) 18, 82, 102, 494, 618, 1172, 1217
BstNI CCWGG 2 cut(s) 313, 639
BstNSI RCATGY 2 cut(s) 558, 1349
BstSCI CCNGG 5 cut(s) 146, 147, 311, 637, 1227
BstSFI CTRYAG 1 cut(s) 1194
BstSLI GKGCMC 1 cut(s) 637
BstV1I GCAGC 4 cut(s) 69, 958, 1180, 1204
BstX2I RGATCY 3 cut(s) 162, 304, 606
BstYI RGATCY 3 cut(s) 162, 304, 606
BstZ17I GTATAC 1 cut(s) 346
BsuI GTATCC 1 cut(s) 407
BsuRI GGCC 3 cut(s) 9, 282, 864
BtsCI GGATG 3 cut(s) 386, 647, 1150
BtsI GCAGTG 1 cut(s) 1203
BtsIMutI CAGTG 3 cut(s) 249, 1003, 1203
Cac8I GCNNGC 2 cut(s) 619, 1347
CaiI CAGNNNCTG 1 cut(s) 1102
CfoI GCGC 1 cut(s) 795
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 4 cut(s) 157, 262, 863, 1099
Cfr9I CCCGGG 1 cut(s) 147
CseI GACGC 2 cut(s) 1006, 1337
Csp6I GTAC 1 cut(s) 592
CspCI CAANNNNNGTGG 2 cut(s) 391, 426
CviAII CATG 3 cut(s) 356, 555, 1346
CviQI GTAC 1 cut(s) 592
DdeI CTNAG 5 cut(s) 962, 975, 1128, 1167, 1308
DinI GGCGCC 1 cut(s) 794
DpnI GATC 3 cut(s) 164, 306, 608
DpnII GATC 3 cut(s) 162, 304, 606
DraI TTTAAA 1 cut(s) 781
DrdI GACNNNNNNGTC 1 cut(s) 149
DriI GACNNNNNGTC 1 cut(s) 548
DseDI GACNNNNNNGTC 1 cut(s) 149
Eam1104I CTCTTC 1 cut(s) 1339
Eam1105I GACNNNNNGTC 1 cut(s) 548
EarI CTCTTC 1 cut(s) 1339
Eco32I GATATC 1 cut(s) 1314
Eco47I GGWCC 3 cut(s) 157, 262, 1099
Eco88I CYCGRG 1 cut(s) 147
EcoNI CCTNNNNNAGG 1 cut(s) 627
EcoO109I RGGNCCY 1 cut(s) 1099
EcoRII CCWGG 2 cut(s) 311, 637
EcoRV GATATC 1 cut(s) 1314
EcoT22I ATGCAT 1 cut(s) 647
EgeI GGCGCC 1 cut(s) 794
EheI GGCGCC 1 cut(s) 794
FaeI CATG 3 cut(s) 359, 558, 1349
FalI AAGNNNNNCTT 2 cut(s) 865, 897
FaqI GGGAC 2 cut(s) 248, 1256
FatI CATG 3 cut(s) 355, 554, 1345
FauI CCCGC 1 cut(s) 1233
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 6 cut(s) 10, 13, 83, 972, 1194, 1218
FokI GGATG 3 cut(s) 393, 654, 1157
Fsp4HI GCNGC 6 cut(s) 10, 13, 83, 972, 1194, 1218
FspBI CTAG 3 cut(s) 239, 302, 548
GlaI GCGC 1 cut(s) 794
GluI GCNGC 6 cut(s) 10, 13, 83, 972, 1194, 1218
HaeII RGCGCY 1 cut(s) 796
HaeIII GGCC 3 cut(s) 9, 282, 864
HapII CCGG 5 cut(s) 77, 148, 160, 1163, 1228
HgaI GACGC 2 cut(s) 1006, 1337
HhaI GCGC 1 cut(s) 795
Hin1I GRCGYC 2 cut(s) 793, 1329
Hin1II CATG 3 cut(s) 359, 558, 1349
Hin6I GCGC 1 cut(s) 793
HinP1I GCGC 1 cut(s) 793
HinfI GANTC 5 cut(s) 653, 854, 979, 1169, 1281
HpaII CCGG 5 cut(s) 77, 148, 160, 1163, 1228
HphI GGTGA 2 cut(s) 484, 974
Hpy166II GTNNAC 4 cut(s) 346, 542, 635, 717
Hpy188I TCNGA 5 cut(s) 61, 380, 965, 1081, 1108
Hpy188III TCNNGA 3 cut(s) 160, 187, 302
Hpy8I GTNNAC 4 cut(s) 346, 542, 635, 717
HpyAV CCTTC 5 cut(s) 318, 388, 455, 677, 1285
HpyCH4III ACNGT 6 cut(s) 675, 934, 998, 1076, 1159, 1252
HpyCH4IV ACGT 2 cut(s) 331, 594
HpyF10VI GCNNNNNNNGC 7 cut(s) 18, 82, 102, 494, 618, 1172, 1217
HpyF3I CTNAG 5 cut(s) 962, 975, 1128, 1167, 1308
HpySE526I ACGT 2 cut(s) 331, 594
Hsp92I GRCGYC 2 cut(s) 793, 1329
Hsp92II CATG 3 cut(s) 359, 558, 1349
HspAI GCGC 1 cut(s) 793
KasI GGCGCC 1 cut(s) 792
Kpn2I TCCGGA 1 cut(s) 159
Kzo9I GATC 3 cut(s) 162, 304, 606
LmnI GCTCC 3 cut(s) 73, 110, 398
Lsp1109I GCAGC 4 cut(s) 69, 958, 1180, 1204
LweI GCATC 2 cut(s) 632, 654
MaeI CTAG 3 cut(s) 239, 302, 548
MaeII ACGT 2 cut(s) 331, 594
MaeIII GTNAC 4 cut(s) 550, 564, 695, 1070
MalI GATC 3 cut(s) 164, 306, 608
MboI GATC 3 cut(s) 162, 304, 606
MboII GAAGA 3 cut(s) 202, 902, 1326
MfeI CAATTG 2 cut(s) 291, 867
MflI RGATCY 3 cut(s) 162, 304, 606
MhlI GDGCHC 1 cut(s) 637
Mly113I GGCGCC 1 cut(s) 793
MlyI GAGTC 4 cut(s) 863, 973, 1178, 1290
MmeI TCCRAC 2 cut(s) 84, 358
Mph1103I ATGCAT 1 cut(s) 647
MroI TCCGGA 1 cut(s) 159
MroXI GAANNNNTTC 2 cut(s) 876, 1158
MseI TTAA 5 cut(s) 455, 468, 662, 780, 948
MslI CAYNNNNRTG 4 cut(s) 68, 576, 640, 1350
MspA1I CMGCKG 2 cut(s) 12, 730
MspCI CTTAAG 1 cut(s) 467
MspI CCGG 5 cut(s) 77, 148, 160, 1163, 1228
MspR9I CCNGG 5 cut(s) 148, 149, 313, 639, 1229
MunI CAATTG 2 cut(s) 291, 867
Mva1269I GAATGC 2 cut(s) 529, 876
MvaI CCWGG 2 cut(s) 313, 639
MwoI GCNNNNNNNGC 7 cut(s) 18, 82, 102, 494, 618, 1172, 1217
NarI GGCGCC 1 cut(s) 793
NciI CCSGG 3 cut(s) 148, 149, 1229
NdeII GATC 3 cut(s) 162, 304, 606
NlaIII CATG 3 cut(s) 359, 558, 1349
NlaIV GGNNCC 8 cut(s) 75, 106, 158, 264, 400, 794, 1161, 1226
NmuCI GTSAC 2 cut(s) 550, 564
NsiI ATGCAT 1 cut(s) 647
NspI RCATGY 2 cut(s) 558, 1349
NspV TTCGAA 2 cut(s) 36, 587
OliI CACNNNNGTG 1 cut(s) 68
PaeI GCATGC 1 cut(s) 1349
PciI ACATGT 1 cut(s) 554
PctI GAATGC 2 cut(s) 529, 876
PdmI GAANNNNTTC 2 cut(s) 876, 1158
PfeI GAWTC 1 cut(s) 653
PflMI CCANNNNNTGG 1 cut(s) 733
PkrI GCNGC 6 cut(s) 11, 14, 84, 973, 1195, 1219
PleI GAGTC 4 cut(s) 862, 973, 1177, 1289
PluTI GGCGCC 1 cut(s) 796
PpsI GAGTC 4 cut(s) 862, 973, 1177, 1289
Ppu21I YACGTR 1 cut(s) 595
PpuMI RGGWCCY 1 cut(s) 1099
PscI ACATGT 1 cut(s) 554
Psp5II RGGWCCY 1 cut(s) 1099
Psp6I CCWGG 2 cut(s) 311, 637
PspGI CCWGG 2 cut(s) 311, 637
PspN4I GGNNCC 8 cut(s) 75, 106, 158, 264, 400, 794, 1161, 1226
PspPI GGNCC 4 cut(s) 157, 262, 863, 1099
PspPPI RGGWCCY 1 cut(s) 1099
PstI CTGCAG 1 cut(s) 1198
PstNI CAGNNNCTG 1 cut(s) 1102
PsuI RGATCY 3 cut(s) 162, 304, 606
PvuII CAGCTG 1 cut(s) 730
RsaI GTAC 1 cut(s) 593
RsaNI GTAC 1 cut(s) 592
RseI CAYNNNNRTG 4 cut(s) 68, 576, 640, 1350
SaqAI TTAA 5 cut(s) 455, 468, 662, 780, 948
SatI GCNGC 6 cut(s) 10, 13, 83, 972, 1194, 1218
Sau3AI GATC 3 cut(s) 162, 304, 606
Sau96I GGNCC 4 cut(s) 157, 262, 863, 1099
SchI GAGTC 4 cut(s) 863, 973, 1178, 1290
ScrFI CCNGG 5 cut(s) 148, 149, 313, 639, 1229
SduI GDGCHC 1 cut(s) 637
SfaNI GCATC 2 cut(s) 632, 654
SfcI CTRYAG 1 cut(s) 1194
SfoI GGCGCC 1 cut(s) 794
SfuI TTCGAA 2 cut(s) 36, 587
SinI GGWCC 3 cut(s) 157, 262, 1099
SmaI CCCGGG 1 cut(s) 149
SmiI ATTTAAAT 1 cut(s) 781
SmiMI CAYNNNNRTG 4 cut(s) 68, 576, 640, 1350
SmlI CTYRAG 1 cut(s) 467
SmoI CTYRAG 1 cut(s) 467
SphI GCATGC 1 cut(s) 1349
SsiI CCGC 3 cut(s) 10, 49, 1240
SspDI GGCGCC 1 cut(s) 792
SspMI CTAG 3 cut(s) 239, 302, 548
StyD4I CCNGG 5 cut(s) 146, 147, 311, 637, 1227
SwaI ATTTAAAT 1 cut(s) 781
TaaI ACNGT 6 cut(s) 675, 934, 998, 1076, 1159, 1252
TaiI ACGT 2 cut(s) 334, 597
TaqI TCGA 7 cut(s) 30, 36, 141, 430, 587, 1050, 1284
TauI GCSGC 1 cut(s) 12
TfiI GAWTC 1 cut(s) 653
Tru1I TTAA 5 cut(s) 455, 468, 662, 780, 948
Tru9I TTAA 5 cut(s) 455, 468, 662, 780, 948
TscAI CASTG 3 cut(s) 256, 1003, 1203
TseFI GTSAC 2 cut(s) 550, 564
TseI GCWGC 5 cut(s) 12, 82, 971, 1193, 1217
Tsp45I GTSAC 2 cut(s) 550, 564
TspDTI ATGAA 1 cut(s) 1167
TspGWI ACGGA 2 cut(s) 80, 432
TspMI CCCGGG 1 cut(s) 147
TspRI CASTG 3 cut(s) 256, 1003, 1203
Van91I CCANNNNNTGG 1 cut(s) 733
Vha464I CTTAAG 1 cut(s) 467
VneI GTGCAC 1 cut(s) 633
VpaK11BI GGWCC 3 cut(s) 157, 262, 1099
XagI CCTNNNNNAGG 1 cut(s) 627
XapI RAATTY 2 cut(s) 776, 801
XbaI TCTAGA 1 cut(s) 301
XceI RCATGY 2 cut(s) 558, 1349
XcmI CCANNNNNNNNNTGG 1 cut(s) 780
XmaI CCCGGG 1 cut(s) 147
XmiI GTMKAC 1 cut(s) 345
XmnI GAANNNNTTC 2 cut(s) 876, 1158
XspI CTAG 3 cut(s) 239, 302, 548
Zsp2I ATGCAT 1 cut(s) 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.