RLG00000025990

Phospholipase A(1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005852
Physical Location & Seq
Forward (+)
49671829 .. 49676669
4841 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025990

Sequence Viewer

Length: 1362 bp
ATGCTGTGCTTCTGCCCCTGTTTCGACGACAACCACCGCCGGAGTTCCGACGACGTGGACCTCGACCTGGACCTGGACCTGGACCTGGAGCCGGTCCTGCTCATCTCCGGAATCGGAGGTTCCATTCTAACTTCTAAACGGAAAAAACTGGGCTTCGACACTCGGGTCTGGGTACGGATCCTATTGGCTGACTTAGAATTCAGGAAGAAACTCTGGTCTCTCTATAATCCCAAAACAGGTTATACAGAAACTTTAGACAAAGACAATGAAATTGTTGTCCCAGATGATGATTATGGCCTCTATGCAATTGATATTCTTGACCCGTCCCCGTTTGTGAAATGCATACATTTGAAAGAGGTATACTATTTTCATGATATGATAAATATGCTTGTGGGTTGTGGCTATAAGAAGGGAACCACATTGTTTGGATATGGCTATGATTTCCGACAAAGCAACAGAATTGAAAAGTTAATGGAAGATCTTAAAGTGAAATTGGAAACTGCTTACAAGGCTTCTGGGGGTAGAAAAGTAAATATAATTTCGCATTCAATGGGTGGACTGCTAGTCATGTGTTTTATGTCACTTCATCATGAGGCCTTTTCAAAGTATGTGAGTAAATGGATTTGCATCGGTTGCCCTTTTCAAGGTGCACCAGGATGCATCAATGATTCTCTTTTAACTGGACTGCAGTTTGTTGAAGGCTTGGAAAGCTTTTTTTTTGTATCAAGGTGGACTATGCACCAGCTGTTGGTTGAGTGTCCATCAATCTACGAGATGTTGGCCAATCCAGATTTTAATTGGAAAAAGCAGCCGGAAATTCAGGTTTGGAGGAAGCATTCTAAGGATGGGGAAACTTCTATTGAACTGGAATCTTATGGTCCCGTTGAAAGTATTACTTTGTTTAGAGAAGCATTGAGACATAATGAGCTAACTTATGATGGTAAAACTGTAGCTTTGCCATTTAACTTTGCTATTTCCAGATGGGCTACTGAGACTCGCCAAGTTCTCAACAATGCCAAACTACCAGATGGAGTCTGCTTCTATAACATTTATGGAATATCCTTTGACACACCTTTTGATGTTTGTTATGGCTCGAAGGCATCTCCAATTGAAGACTTGTCTGAAATATGCCATTCAATGCCTCAGTATTCTTATGTGGATGGAGATGAAACAGTTTCTGCTGAATCAGCAAAGGCAGATGGATTTGCTGCAGTTGAGAGAGTAGCAATAGCTGCAAGACACCGGGAACTATTAAGTGACAAAACGGTTTTTCAACATATCAAGAAGTGGTTGGGAGTTGAGCAGAAGGTCAACATACATTCCAAGACATCCAGAGTTTCAGATAAGCCTGTGAGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110
Pfam Domains
Protein Families
LCAT

Protein Analysis

454

Amino Acids

51.64

Weight (kDa)

5.69

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 75 - 397 5.5e-24 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
AccB7I CCANNNNNTGG 1 cut(s) 748
AccI GTMKAC 1 cut(s) 360
AccIII TCCGGA 1 cut(s) 107
AciI CCGC 1 cut(s) 37
AclWI GGATC 2 cut(s) 172, 185
AcoI YGGCCR 1 cut(s) 780
AcsI RAATTY 2 cut(s) 197, 816
AfaI GTAC 1 cut(s) 174
AfiI CCNNNNNNNGG 8 cut(s) 67, 73, 79, 85, 91, 236, 644, 748
AhdI GACNNNNNGTC 1 cut(s) 563
AjiI CACGTC 1 cut(s) 55
AjnI CCWGG 5 cut(s) 66, 72, 78, 84, 652
AluBI AGCT 5 cut(s) 711, 745, 928, 953, 1232
AluI AGCT 5 cut(s) 711, 745, 928, 953, 1232
Alw21I GWGCWC 1 cut(s) 652
Alw26I GTCTC 3 cut(s) 222, 910, 986
Alw44I GTGCAC 1 cut(s) 648
AlwI GGATC 2 cut(s) 172, 185
AlwNI CAGNNNCTG 1 cut(s) 1178
Ama87I CYCGRG 1 cut(s) 162
Aor13HI TCCGGA 1 cut(s) 107
AoxI GGCC 3 cut(s) 295, 594, 780
ApaLI GTGCAC 1 cut(s) 648
ApeKI GCWGC 3 cut(s) 808, 1208, 1232
ApoI RAATTY 2 cut(s) 197, 816
ArsI GACNNNNNNTTYG 2 cut(s) 1058, 1090
AspS9I GGNCC 6 cut(s) 58, 70, 76, 82, 94, 878
AsuC2I CCSGG 1 cut(s) 1244
AvaI CYCGRG 1 cut(s) 162
AvaII GGWCC 6 cut(s) 58, 70, 76, 82, 94, 878
BaeGI GKGCMC 1 cut(s) 652
BalI TGGCCA 1 cut(s) 782
BamHI GGATCC 1 cut(s) 177
BbsI GAAGAC 1 cut(s) 1119
Bbv12I GWGCWC 1 cut(s) 652
BbvI GCAGC 3 cut(s) 820, 1195, 1219
BccI CCATC 7 cut(s) 769, 839, 932, 975, 1022, 1154, 1193
BciT130I CCWGG 5 cut(s) 68, 74, 80, 86, 654
BcnI CCSGG 1 cut(s) 1244
BcoDI GTCTC 3 cut(s) 222, 910, 986
BfaI CTAG 1 cut(s) 563
BfmI CTRYAG 3 cut(s) 686, 948, 1209
BglII AGATCT 1 cut(s) 478
BisI GCNGC 3 cut(s) 809, 1209, 1233
BlsI GCNGC 3 cut(s) 810, 1210, 1234
Bme1390I CCNGG 6 cut(s) 68, 74, 80, 86, 654, 1244
Bme18I GGWCC 6 cut(s) 58, 70, 76, 82, 94, 878
BmeRI GACNNNNNGTC 1 cut(s) 563
BmeT110I CYCGRG 1 cut(s) 162
BmgBI CACGTC 1 cut(s) 55
BmgT120I GGNCC 6 cut(s) 58, 70, 76, 82, 94, 878
BmiI GGNNCC 5 cut(s) 90, 121, 179, 415, 880
BmrFI CCNGG 6 cut(s) 68, 74, 80, 86, 654, 1244
BmrI ACTGGG 1 cut(s) 158
BmsI GCATC 4 cut(s) 636, 647, 669, 1109
BmuI ACTGGG 1 cut(s) 158
BpiI GAAGAC 1 cut(s) 1119
BpmI CTGGAG 1 cut(s) 107
BpuMI CCSGG 1 cut(s) 1244
BsaBI GATNNNNATC 1 cut(s) 626
BsaI GGTCTC 1 cut(s) 222
BsaWI WCCGGW 1 cut(s) 107
Bsc4I CCNNNNNNNGG 8 cut(s) 67, 73, 79, 85, 91, 236, 644, 748
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 3 cut(s) 153, 685, 870
Bse8I GATNNNNATC 1 cut(s) 626
BseAI TCCGGA 1 cut(s) 107
BseBI CCWGG 5 cut(s) 68, 74, 80, 86, 654
BseGI GGATG 4 cut(s) 662, 850, 1165, 1328
BseJI GATNNNNATC 1 cut(s) 626
BseLI CCNNNNNNNGG 8 cut(s) 67, 73, 79, 85, 91, 236, 644, 748
BseMII CTCAG 2 cut(s) 981, 1157
BseNI ACTGG 3 cut(s) 153, 685, 870
BseSI GKGCMC 1 cut(s) 652
BseXI GCAGC 3 cut(s) 820, 1195, 1219
BshFI GGCC 3 cut(s) 297, 596, 782
BsiHKAI GWGCWC 1 cut(s) 652
BsiHKCI CYCGRG 1 cut(s) 162
BsiSI CCGG 5 cut(s) 40, 92, 108, 812, 1243
BslFI GGGAC 3 cut(s) 263, 310, 864
BslI CCNNNNNNNGG 8 cut(s) 67, 73, 79, 85, 91, 236, 644, 748
BsmAI GTCTC 3 cut(s) 222, 910, 986
BsmFI GGGAC 3 cut(s) 263, 310, 864
BsmI GAATGC 2 cut(s) 544, 835
BsnI GGCC 3 cut(s) 297, 596, 782
Bso31I GGTCTC 1 cut(s) 222
BsoBI CYCGRG 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 652
Bsp13I TCCGGA 1 cut(s) 107
Bsp143I GATC 2 cut(s) 177, 478
BspACI CCGC 1 cut(s) 37
BspANI GGCC 3 cut(s) 297, 596, 782
BspCNI CTCAG 2 cut(s) 982, 1156
BspEI TCCGGA 1 cut(s) 107
BspHI TCATGA 2 cut(s) 370, 589
BspLI GGNNCC 5 cut(s) 90, 121, 179, 415, 880
BspMAI CTGCAG 2 cut(s) 690, 1213
BspPI GGATC 2 cut(s) 172, 185
BspTNI GGTCTC 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 3 cut(s) 153, 685, 870
BssAI RCCGGY 1 cut(s) 91
BssMI GATC 2 cut(s) 177, 478
BssNAI GTATAC 1 cut(s) 361
Bst1107I GTATAC 1 cut(s) 361
Bst2UI CCWGG 5 cut(s) 68, 74, 80, 86, 654
Bst4CI ACNGT 3 cut(s) 949, 1174, 1267
BstAPI GCANNNNNTGC 2 cut(s) 633, 1232
BstDEI CTNAG 4 cut(s) 193, 840, 990, 1143
BstENI CCTNNNNNAGG 1 cut(s) 642
BstF5I GGATG 4 cut(s) 662, 850, 1165, 1328
BstKTI GATC 2 cut(s) 180, 481
BstMAI GTCTC 3 cut(s) 222, 910, 986
BstMBI GATC 2 cut(s) 177, 478
BstMWI GCNNNNNNNGC 6 cut(s) 97, 509, 633, 708, 1187, 1232
BstNI CCWGG 5 cut(s) 68, 74, 80, 86, 654
BstSCI CCNGG 6 cut(s) 66, 72, 78, 84, 652, 1242
BstSFI CTRYAG 3 cut(s) 686, 948, 1209
BstSLI GKGCMC 1 cut(s) 652
BstV1I GCAGC 3 cut(s) 820, 1195, 1219
BstV2I GAAGAC 1 cut(s) 1119
BstX2I RGATCY 2 cut(s) 177, 478
BstYI RGATCY 2 cut(s) 177, 478
BstZ17I GTATAC 1 cut(s) 361
BsuRI GGCC 3 cut(s) 297, 596, 782
BtrI CACGTC 1 cut(s) 55
BtsCI GGATG 4 cut(s) 662, 850, 1165, 1328
CaiI CAGNNNCTG 1 cut(s) 1178
CciI TCATGA 2 cut(s) 370, 589
Cfr10I RCCGGY 1 cut(s) 91
Cfr13I GGNCC 6 cut(s) 58, 70, 76, 82, 94, 878
Csp6I GTAC 1 cut(s) 173
CspCI CAANNNNNGTGG 2 cut(s) 406, 441
CviAII CATG 3 cut(s) 371, 568, 590
CviQI GTAC 1 cut(s) 173
DdeI CTNAG 4 cut(s) 193, 840, 990, 1143
DpnI GATC 2 cut(s) 179, 480
DpnII GATC 2 cut(s) 177, 478
DrdI GACNNNNNNGTC 1 cut(s) 164
DriI GACNNNNNGTC 1 cut(s) 563
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 1 cut(s) 780
Eam1105I GACNNNNNGTC 1 cut(s) 563
Eco147I AGGCCT 1 cut(s) 596
Eco31I GGTCTC 1 cut(s) 222
Eco47I GGWCC 6 cut(s) 58, 70, 76, 82, 94, 878
Eco88I CYCGRG 1 cut(s) 162
EcoNI CCTNNNNNAGG 1 cut(s) 642
EcoRI GAATTC 1 cut(s) 197
EcoRII CCWGG 5 cut(s) 66, 72, 78, 84, 652
EcoT22I ATGCAT 2 cut(s) 344, 662
FaeI CATG 3 cut(s) 374, 571, 593
FalI AAGNNNNNCTT 2 cut(s) 880, 912
FaqI GGGAC 3 cut(s) 263, 310, 864
FatI CATG 3 cut(s) 370, 567, 589
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 3 cut(s) 809, 1209, 1233
FokI GGATG 4 cut(s) 669, 857, 1172, 1315
Fsp4HI GCNGC 3 cut(s) 809, 1209, 1233
FspBI CTAG 1 cut(s) 563
GluI GCNGC 3 cut(s) 809, 1209, 1233
GsuI CTGGAG 1 cut(s) 107
HaeIII GGCC 3 cut(s) 297, 596, 782
HapII CCGG 5 cut(s) 40, 92, 108, 812, 1243
Hin1II CATG 3 cut(s) 374, 571, 593
HincII GTYRAC 1 cut(s) 1312
HindII GTYRAC 1 cut(s) 1312
HindIII AAGCTT 1 cut(s) 709
HinfI GANTC 6 cut(s) 111, 668, 869, 994, 1032, 1184
HpaII CCGG 5 cut(s) 40, 92, 108, 812, 1243
Hpy166II GTNNAC 6 cut(s) 58, 361, 557, 650, 732, 1312
Hpy188I TCNGA 5 cut(s) 49, 116, 446, 1123, 1342
Hpy188III TCNNGA 9 cut(s) 108, 202, 317, 371, 590, 788, 978, 1282, 1332
Hpy8I GTNNAC 6 cut(s) 58, 361, 557, 650, 732, 1312
Hpy99I CGWCG 3 cut(s) 29, 53, 56
HpyAV CCTTC 4 cut(s) 403, 692, 1090, 1300
HpyCH4III ACNGT 3 cut(s) 949, 1174, 1267
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 9 cut(s) 305, 342, 627, 650, 660, 688, 739, 1211, 1235
HpyF10VI GCNNNNNNNGC 6 cut(s) 97, 509, 633, 708, 1187, 1232
HpyF3I CTNAG 4 cut(s) 193, 840, 990, 1143
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 3 cut(s) 374, 571, 593
Kpn2I TCCGGA 1 cut(s) 107
Kzo9I GATC 2 cut(s) 177, 478
LmnI GCTCC 1 cut(s) 88
Lsp1109I GCAGC 3 cut(s) 820, 1195, 1219
LweI GCATC 4 cut(s) 636, 647, 669, 1109
MaeI CTAG 1 cut(s) 563
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 2 cut(s) 579, 1256
MalI GATC 2 cut(s) 179, 480
MboI GATC 2 cut(s) 177, 478
MboII GAAGA 3 cut(s) 217, 488, 1124
MfeI CAATTG 2 cut(s) 306, 1107
MflI RGATCY 2 cut(s) 177, 478
MhlI GDGCHC 1 cut(s) 652
MlsI TGGCCA 1 cut(s) 782
MluCI AATT 9 cut(s) 197, 270, 306, 459, 491, 537, 796, 816, 1107
MluNI TGGCCA 1 cut(s) 782
MlyI GAGTC 2 cut(s) 988, 1041
MmeI TCCRAC 2 cut(s) 72, 469
MnlI CCTC 8 cut(s) 71, 110, 308, 349, 586, 822, 1152, 1347
Mox20I TGGCCA 1 cut(s) 782
Mph1103I ATGCAT 2 cut(s) 344, 662
MroI TCCGGA 1 cut(s) 107
MscI TGGCCA 1 cut(s) 782
MseI TTAA 6 cut(s) 470, 483, 677, 795, 963, 1253
MslI CAYNNNNRTG 1 cut(s) 655
Msp20I TGGCCA 1 cut(s) 782
MspA1I CMGCKG 1 cut(s) 745
MspI CCGG 5 cut(s) 40, 92, 108, 812, 1243
MspR9I CCNGG 6 cut(s) 68, 74, 80, 86, 654, 1244
MunI CAATTG 2 cut(s) 306, 1107
Mva1269I GAATGC 2 cut(s) 544, 835
MvaI CCWGG 5 cut(s) 68, 74, 80, 86, 654
MwoI GCNNNNNNNGC 6 cut(s) 97, 509, 633, 708, 1187, 1232
NciI CCSGG 1 cut(s) 1244
NdeII GATC 2 cut(s) 177, 478
NlaIII CATG 3 cut(s) 374, 571, 593
NlaIV GGNNCC 5 cut(s) 90, 121, 179, 415, 880
NmuCI GTSAC 2 cut(s) 579, 1256
NsiI ATGCAT 2 cut(s) 344, 662
PagI TCATGA 2 cut(s) 370, 589
PceI AGGCCT 1 cut(s) 596
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PctI GAATGC 2 cut(s) 544, 835
PfeI GAWTC 4 cut(s) 111, 668, 869, 1184
PflMI CCANNNNNTGG 1 cut(s) 748
PkrI GCNGC 3 cut(s) 810, 1210, 1234
PleI GAGTC 2 cut(s) 988, 1040
PpsI GAGTC 2 cut(s) 988, 1040
Psp6I CCWGG 5 cut(s) 66, 72, 78, 84, 652
PspGI CCWGG 5 cut(s) 66, 72, 78, 84, 652
PspN4I GGNNCC 5 cut(s) 90, 121, 179, 415, 880
PspPI GGNCC 6 cut(s) 58, 70, 76, 82, 94, 878
PstI CTGCAG 2 cut(s) 690, 1213
PstNI CAGNNNCTG 1 cut(s) 1178
PsuI RGATCY 2 cut(s) 177, 478
PvuII CAGCTG 1 cut(s) 745
RsaI GTAC 1 cut(s) 174
RsaNI GTAC 1 cut(s) 173
RseI CAYNNNNRTG 1 cut(s) 655
SaqAI TTAA 6 cut(s) 470, 483, 677, 795, 963, 1253
SatI GCNGC 3 cut(s) 809, 1209, 1233
Sau3AI GATC 2 cut(s) 177, 478
Sau96I GGNCC 6 cut(s) 58, 70, 76, 82, 94, 878
SchI GAGTC 2 cut(s) 988, 1041
ScrFI CCNGG 6 cut(s) 68, 74, 80, 86, 654, 1244
SduI GDGCHC 1 cut(s) 652
SfaNI GCATC 4 cut(s) 636, 647, 669, 1109
SfcI CTRYAG 3 cut(s) 686, 948, 1209
SinI GGWCC 6 cut(s) 58, 70, 76, 82, 94, 878
SmiMI CAYNNNNRTG 1 cut(s) 655
Sse9I AATT 9 cut(s) 197, 270, 306, 459, 491, 537, 796, 816, 1107
SseBI AGGCCT 1 cut(s) 596
SsiI CCGC 1 cut(s) 37
SspMI CTAG 1 cut(s) 563
StuI AGGCCT 1 cut(s) 596
StyD4I CCNGG 6 cut(s) 66, 72, 78, 84, 652, 1242
TaaI ACNGT 3 cut(s) 949, 1174, 1267
TaiI ACGT 1 cut(s) 57
TaqI TCGA 4 cut(s) 24, 63, 156, 1094
TasI AATT 9 cut(s) 197, 270, 306, 459, 491, 537, 796, 816, 1107
TfiI GAWTC 4 cut(s) 111, 668, 869, 1184
Tru1I TTAA 6 cut(s) 470, 483, 677, 795, 963, 1253
Tru9I TTAA 6 cut(s) 470, 483, 677, 795, 963, 1253
TseFI GTSAC 2 cut(s) 579, 1256
TseI GCWGC 3 cut(s) 808, 1208, 1232
Tsp45I GTSAC 2 cut(s) 579, 1256
TspDTI ATGAA 4 cut(s) 282, 359, 575, 1182
TspGWI ACGGA 2 cut(s) 154, 190
Van91I CCANNNNNTGG 1 cut(s) 748
VneI GTGCAC 1 cut(s) 648
VpaK11BI GGWCC 6 cut(s) 58, 70, 76, 82, 94, 878
XagI CCTNNNNNAGG 1 cut(s) 642
XapI RAATTY 2 cut(s) 197, 816
XcmI CCANNNNNNNNNTGG 1 cut(s) 795
XmiI GTMKAC 1 cut(s) 360
XspI CTAG 1 cut(s) 563
Zsp2I ATGCAT 2 cut(s) 344, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.