Rh3AG003000

Phospholipase A(1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0005852
Physical Location & Seq
Reverse (-)
254449 .. 258140
3692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG003000.1

Sequence Viewer

Length: 1245 bp
ATGGTTATATCTCTCTCTCCATGTTTCCAGTTTGTGAAATGCATACATTTGAAAGAGGTATACTATTTTCATGATATGATCAATATGCTTGTGGGTTGTGGCTATAAGAAGGGAACTACATTGTTTGGATATGGCTATGATTTCCGACAAAGCAATAGAATTGAAAAGTTAATGGAAGATCTTAAAGTGAAATTGGAAACTGCTTACAAGGCTTCTGGTGGTAGAAAAGTAAATATAATTTCGCATTCAATGGGTGGACTGCTAGTCATGTGTTTTATGTCACTTCATCATGAGGCCTTTTCAAAATATGTGAGTAAATGGATTTGCATCGGTTGCCCTTTTCAAGGTGCACCAGGATGCATCAATGATTCTCTTTTAACTGGACTGCAGTTTGTTGAAGGCTTGGAAAGCTTTTTTTTTGTATCAAGGTGGACTATGCACCAGCTGTTGGTTGAGTGTCCATCAATCTACGAGATGTTGGCCAATCCAGATTTTAATTGGAAAAAGCAGCCGGAAATTCAGGTTTGGAGGAAGCATTCTAAGGATGGGGAAACTTCTATTGAACTGGAATCTTATGGTCCCGTTGAAAGTATTACTTTGTTTAGAGAAGCATTGAGACATAATGAGCTAACTTATGATGGTAAAACTATAGCTTTGCCATTTAACTTTGCTATTTCCAGATGGGCTGCCGAGACTCGCCAAGTTCTCAACAATGCCAAACTACCAGATGGAGTCTGCTTCTATAACATTTATGGAATATCCTTTGACACACCTTTTGATGTTTGTTATGGCTCGAAGGCATCTCCAATTGAAGACTTGTCTGAAATATGCCATTCAATGCCTCAGTATTCTTATGTGGATGGAGATGAAACAGTTTCTGCAGAATCAGCAAAGGTGTTGTATCTGACTCTTCATATTATGTTGCAGACTTGTATTCTTAGTTTCTACTTTCTAGCCAGTGTTGAGCCATACTCTGGCTATGACGGCTTTATTTGTAAGTTACTGCTTTGGAGCAAGTTTGATAATGGTTCCGGCCTGCATTGGAGTGAATTAAACTTGTTTCTTCGTATTGCGCAGGCAGATGGATTTGCTGCAGTTGAAAGAGTAGCAATAGCTGCAAGACACCGGGACCTATTAAGTGACAAAACGGTTTTTCAACATATCAAGAAGTGGTTGGGAGTTGAGCAGAAGGTCAACATACATTCCAAGACATCCAGAGTTTCAGATAAGCCTGTGAGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110
Pfam Domains
Protein Families
LCAT

Protein Analysis

414

Amino Acids

47.22

Weight (kDa)

6.54

Isoelectric Point (pI)

45.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 19 - 299 2e-21 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1074
AccB7I CCANNNNNTGG 2 cut(s) 448, 974
AccI GTMKAC 1 cut(s) 60
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 1 cut(s) 516
AfiI CCNNNNNNNGG 3 cut(s) 344, 448, 974
AhdI GACNNNNNGTC 1 cut(s) 263
AjnI CCWGG 1 cut(s) 352
AluBI AGCT 5 cut(s) 411, 445, 628, 653, 1115
AluI AGCT 5 cut(s) 411, 445, 628, 653, 1115
Alw21I GWGCWC 1 cut(s) 352
Alw26I GTCTC 2 cut(s) 610, 686
Alw44I GTGCAC 1 cut(s) 348
AlwNI CAGNNNCTG 1 cut(s) 878
AoxI GGCC 3 cut(s) 294, 480, 1033
ApaLI GTGCAC 1 cut(s) 348
ApeKI GCWGC 4 cut(s) 508, 686, 1091, 1115
ApoI RAATTY 1 cut(s) 516
ArsI GACNNNNNNTTYG 2 cut(s) 758, 790
AspLEI GCGC 1 cut(s) 1075
AspS9I GGNCC 2 cut(s) 578, 1129
AsuC2I CCSGG 1 cut(s) 1127
AvaII GGWCC 2 cut(s) 578, 1129
BaeGI GKGCMC 1 cut(s) 352
BalI TGGCCA 1 cut(s) 482
BbsI GAAGAC 1 cut(s) 819
Bbv12I GWGCWC 1 cut(s) 352
BbvI GCAGC 4 cut(s) 520, 673, 1078, 1102
BccI CCATC 7 cut(s) 469, 539, 632, 675, 722, 854, 1076
BceAI ACGGC 1 cut(s) 1000
BciT130I CCWGG 1 cut(s) 354
BclI TGATCA 1 cut(s) 78
BcnI CCSGG 1 cut(s) 1127
BcoDI GTCTC 2 cut(s) 610, 686
BfaI CTAG 2 cut(s) 263, 953
BfmI CTRYAG 4 cut(s) 386, 648, 879, 1092
BglII AGATCT 1 cut(s) 178
BisI GCNGC 4 cut(s) 509, 687, 1092, 1116
BlsI GCNGC 4 cut(s) 510, 688, 1093, 1117
Bme1390I CCNGG 2 cut(s) 354, 1127
Bme18I GGWCC 2 cut(s) 578, 1129
BmeRI GACNNNNNGTC 1 cut(s) 263
BmgT120I GGNCC 2 cut(s) 578, 1129
BmiI GGNNCC 3 cut(s) 580, 1030, 1130
BmrFI CCNGG 2 cut(s) 354, 1127
BmsI GCATC 4 cut(s) 336, 347, 369, 809
BpiI GAAGAC 1 cut(s) 819
BplI GAGNNNNNCTC 2 cut(s) 956, 988
BpuMI CCSGG 1 cut(s) 1127
BsaBI GATNNNNATC 1 cut(s) 326
Bsc4I CCNNNNNNNGG 3 cut(s) 344, 448, 974
Bse1I ACTGG 4 cut(s) 28, 385, 570, 957
Bse8I GATNNNNATC 1 cut(s) 326
BseBI CCWGG 1 cut(s) 354
BseGI GGATG 4 cut(s) 362, 550, 865, 1211
BseJI GATNNNNATC 1 cut(s) 326
BseLI CCNNNNNNNGG 3 cut(s) 344, 448, 974
BseMII CTCAG 1 cut(s) 857
BseNI ACTGG 4 cut(s) 28, 385, 570, 957
BseSI GKGCMC 1 cut(s) 352
BseXI GCAGC 4 cut(s) 520, 673, 1078, 1102
BshFI GGCC 3 cut(s) 296, 482, 1035
BsiHKAI GWGCWC 1 cut(s) 352
BsiSI CCGG 3 cut(s) 512, 1032, 1126
BslFI GGGAC 2 cut(s) 564, 1142
BslI CCNNNNNNNGG 3 cut(s) 344, 448, 974
BsmAI GTCTC 2 cut(s) 610, 686
BsmFI GGGAC 2 cut(s) 564, 1142
BsmI GAATGC 2 cut(s) 244, 535
BsnI GGCC 3 cut(s) 296, 482, 1035
Bsp1286I GDGCHC 1 cut(s) 352
Bsp143I GATC 2 cut(s) 78, 178
BspANI GGCC 3 cut(s) 296, 482, 1035
BspCNI CTCAG 1 cut(s) 856
BspHI TCATGA 2 cut(s) 70, 289
BspLI GGNNCC 3 cut(s) 580, 1030, 1130
BspMAI CTGCAG 3 cut(s) 390, 883, 1096
BsrI ACTGG 4 cut(s) 28, 385, 570, 957
BssMI GATC 2 cut(s) 78, 178
BssNAI GTATAC 1 cut(s) 61
Bst1107I GTATAC 1 cut(s) 61
Bst2UI CCWGG 1 cut(s) 354
Bst4CI ACNGT 2 cut(s) 874, 1150
Bst6I CTCTTC 1 cut(s) 915
BstAPI GCANNNNNTGC 2 cut(s) 333, 1115
BstC8I GCNNGC 2 cut(s) 1037, 1077
BstDEI CTNAG 3 cut(s) 540, 843, 938
BstENI CCTNNNNNAGG 1 cut(s) 342
BstF5I GGATG 4 cut(s) 362, 550, 865, 1211
BstHHI GCGC 1 cut(s) 1075
BstKTI GATC 2 cut(s) 81, 181
BstMAI GTCTC 2 cut(s) 610, 686
BstMBI GATC 2 cut(s) 78, 178
BstMWI GCNNNNNNNGC 6 cut(s) 209, 333, 408, 887, 984, 1115
BstNI CCWGG 1 cut(s) 354
BstSCI CCNGG 2 cut(s) 352, 1125
BstSFI CTRYAG 4 cut(s) 386, 648, 879, 1092
BstSLI GKGCMC 1 cut(s) 352
BstV1I GCAGC 4 cut(s) 520, 673, 1078, 1102
BstV2I GAAGAC 1 cut(s) 819
BstX2I RGATCY 1 cut(s) 178
BstYI RGATCY 1 cut(s) 178
BstZ17I GTATAC 1 cut(s) 61
BsuRI GGCC 3 cut(s) 296, 482, 1035
BtsCI GGATG 4 cut(s) 362, 550, 865, 1211
BtsIMutI CAGTG 1 cut(s) 964
Cac8I GCNNGC 2 cut(s) 1037, 1077
CaiI CAGNNNCTG 1 cut(s) 878
CciI TCATGA 2 cut(s) 70, 289
CfoI GCGC 1 cut(s) 1075
Cfr13I GGNCC 2 cut(s) 578, 1129
CviAII CATG 4 cut(s) 21, 71, 268, 290
DdeI CTNAG 3 cut(s) 540, 843, 938
DpnI GATC 2 cut(s) 80, 180
DpnII GATC 2 cut(s) 78, 178
DriI GACNNNNNGTC 1 cut(s) 263
EaeI YGGCCR 1 cut(s) 480
Eam1104I CTCTTC 1 cut(s) 915
Eam1105I GACNNNNNGTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 915
Eco147I AGGCCT 1 cut(s) 296
Eco47I GGWCC 2 cut(s) 578, 1129
EcoNI CCTNNNNNAGG 1 cut(s) 342
EcoO109I RGGNCCY 1 cut(s) 1129
EcoRII CCWGG 1 cut(s) 352
EcoT22I ATGCAT 2 cut(s) 44, 362
FaeI CATG 4 cut(s) 24, 74, 271, 293
FalI AAGNNNNNCTT 2 cut(s) 580, 612
FaqI GGGAC 2 cut(s) 564, 1142
FatI CATG 4 cut(s) 20, 70, 267, 289
FbaI TGATCA 1 cut(s) 78
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 4 cut(s) 509, 687, 1092, 1116
FokI GGATG 4 cut(s) 369, 557, 872, 1198
Fsp4HI GCNGC 4 cut(s) 509, 687, 1092, 1116
FspBI CTAG 2 cut(s) 263, 953
FspI TGCGCA 1 cut(s) 1074
GlaI GCGC 1 cut(s) 1074
GluI GCNGC 4 cut(s) 509, 687, 1092, 1116
HaeIII GGCC 3 cut(s) 296, 482, 1035
HapII CCGG 3 cut(s) 512, 1032, 1126
HhaI GCGC 1 cut(s) 1075
Hin1II CATG 4 cut(s) 24, 74, 271, 293
Hin6I GCGC 1 cut(s) 1073
HinP1I GCGC 1 cut(s) 1073
HincII GTYRAC 1 cut(s) 1195
HindII GTYRAC 1 cut(s) 1195
HindIII AAGCTT 1 cut(s) 409
HinfI GANTC 6 cut(s) 368, 569, 694, 732, 884, 907
HpaII CCGG 3 cut(s) 512, 1032, 1126
Hpy166II GTNNAC 5 cut(s) 61, 257, 350, 432, 1195
Hpy188I TCNGA 4 cut(s) 146, 823, 906, 1225
Hpy188III TCNNGA 6 cut(s) 71, 290, 488, 678, 1165, 1215
Hpy8I GTNNAC 5 cut(s) 61, 257, 350, 432, 1195
HpyAV CCTTC 4 cut(s) 103, 392, 790, 1183
HpyCH4III ACNGT 2 cut(s) 874, 1150
HpyF10VI GCNNNNNNNGC 6 cut(s) 209, 333, 408, 887, 984, 1115
HpyF3I CTNAG 3 cut(s) 540, 843, 938
Hsp92II CATG 4 cut(s) 24, 74, 271, 293
HspAI GCGC 1 cut(s) 1073
Ksp22I TGATCA 1 cut(s) 78
Kzo9I GATC 2 cut(s) 78, 178
LmnI GCTCC 1 cut(s) 1011
Lsp1109I GCAGC 4 cut(s) 520, 673, 1078, 1102
LweI GCATC 4 cut(s) 336, 347, 369, 809
MaeI CTAG 2 cut(s) 263, 953
MaeIII GTNAC 3 cut(s) 279, 999, 1139
MalI GATC 2 cut(s) 80, 180
MboI GATC 2 cut(s) 78, 178
MboII GAAGA 4 cut(s) 188, 824, 902, 1055
MfeI CAATTG 1 cut(s) 807
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 352
MlsI TGGCCA 1 cut(s) 482
MluCI AATT 7 cut(s) 159, 191, 237, 496, 516, 807, 1049
MluNI TGGCCA 1 cut(s) 482
MlyI GAGTC 3 cut(s) 688, 741, 901
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 5 cut(s) 49, 286, 522, 852, 1230
Mox20I TGGCCA 1 cut(s) 482
Mph1103I ATGCAT 2 cut(s) 44, 362
MscI TGGCCA 1 cut(s) 482
MseI TTAA 7 cut(s) 170, 183, 377, 495, 663, 1052, 1136
MslI CAYNNNNRTG 2 cut(s) 355, 1044
Msp20I TGGCCA 1 cut(s) 482
MspA1I CMGCKG 1 cut(s) 445
MspI CCGG 3 cut(s) 512, 1032, 1126
MspR9I CCNGG 2 cut(s) 354, 1127
MunI CAATTG 1 cut(s) 807
Mva1269I GAATGC 2 cut(s) 244, 535
MvaI CCWGG 1 cut(s) 354
MwoI GCNNNNNNNGC 6 cut(s) 209, 333, 408, 887, 984, 1115
NciI CCSGG 1 cut(s) 1127
NdeII GATC 2 cut(s) 78, 178
NlaIII CATG 4 cut(s) 24, 74, 271, 293
NlaIV GGNNCC 3 cut(s) 580, 1030, 1130
NmeAIII GCCGAG 1 cut(s) 715
NmuCI GTSAC 2 cut(s) 279, 1139
NsbI TGCGCA 1 cut(s) 1074
NsiI ATGCAT 2 cut(s) 44, 362
PagI TCATGA 2 cut(s) 70, 289
PceI AGGCCT 1 cut(s) 296
PctI GAATGC 2 cut(s) 244, 535
PfeI GAWTC 3 cut(s) 368, 569, 884
PflMI CCANNNNNTGG 2 cut(s) 448, 974
PkrI GCNGC 4 cut(s) 510, 688, 1093, 1117
PleI GAGTC 3 cut(s) 688, 740, 901
PpsI GAGTC 3 cut(s) 688, 740, 901
PpuMI RGGWCCY 1 cut(s) 1129
Psp5II RGGWCCY 1 cut(s) 1129
Psp6I CCWGG 1 cut(s) 352
PspGI CCWGG 1 cut(s) 352
PspN4I GGNNCC 3 cut(s) 580, 1030, 1130
PspPI GGNCC 2 cut(s) 578, 1129
PspPPI RGGWCCY 1 cut(s) 1129
PstI CTGCAG 3 cut(s) 390, 883, 1096
PstNI CAGNNNCTG 1 cut(s) 878
PsuI RGATCY 1 cut(s) 178
PvuII CAGCTG 1 cut(s) 445
RseI CAYNNNNRTG 2 cut(s) 355, 1044
SaqAI TTAA 7 cut(s) 170, 183, 377, 495, 663, 1052, 1136
SatI GCNGC 4 cut(s) 509, 687, 1092, 1116
Sau3AI GATC 2 cut(s) 78, 178
Sau96I GGNCC 2 cut(s) 578, 1129
SchI GAGTC 3 cut(s) 688, 741, 901
ScrFI CCNGG 2 cut(s) 354, 1127
SduI GDGCHC 1 cut(s) 352
SfaNI GCATC 4 cut(s) 336, 347, 369, 809
SfcI CTRYAG 4 cut(s) 386, 648, 879, 1092
SinI GGWCC 2 cut(s) 578, 1129
SmiMI CAYNNNNRTG 2 cut(s) 355, 1044
Sse9I AATT 7 cut(s) 159, 191, 237, 496, 516, 807, 1049
SseBI AGGCCT 1 cut(s) 296
SspMI CTAG 2 cut(s) 263, 953
StuI AGGCCT 1 cut(s) 296
StyD4I CCNGG 2 cut(s) 352, 1125
TaaI ACNGT 2 cut(s) 874, 1150
TaqI TCGA 1 cut(s) 794
TasI AATT 7 cut(s) 159, 191, 237, 496, 516, 807, 1049
TfiI GAWTC 3 cut(s) 368, 569, 884
Tru1I TTAA 7 cut(s) 170, 183, 377, 495, 663, 1052, 1136
Tru9I TTAA 7 cut(s) 170, 183, 377, 495, 663, 1052, 1136
TscAI CASTG 1 cut(s) 964
TseFI GTSAC 2 cut(s) 279, 1139
TseI GCWGC 4 cut(s) 508, 686, 1091, 1115
Tsp45I GTSAC 2 cut(s) 279, 1139
TspDTI ATGAA 4 cut(s) 59, 275, 882, 902
TspRI CASTG 1 cut(s) 964
Van91I CCANNNNNTGG 2 cut(s) 448, 974
VneI GTGCAC 1 cut(s) 348
VpaK11BI GGWCC 2 cut(s) 578, 1129
XagI CCTNNNNNAGG 1 cut(s) 342
XapI RAATTY 1 cut(s) 516
XcmI CCANNNNNNNNNTGG 1 cut(s) 495
XmiI GTMKAC 1 cut(s) 60
XspI CTAG 2 cut(s) 263, 953
Zsp2I ATGCAT 2 cut(s) 44, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.